Kit for lung cancer detection

Abstract

The present invention discloses a lung cancer detection kit, and relates to the field of lung cancer detection kits, the lung cancer detection kit comprises a box body and a sealing box cover arranged on the box body, the interior of the box body is divided into a normal temperature part and a refrigeration part, the top of the refrigeration part is provided with a pick-and-place cavity, the bottom of the refrigeration part is provided with a cold source cavity, and the cold source cavity is provided with a cold source. And a cold source box is arranged in the cold source cavity. According to the kit for lung cancer detection, in the reagent transportation process, cold air in the cold source cavity is in direct contact with a reagent bottle to cool and refrigerate the reagent in the reagent bottle, and leakage of the cold air in the cold source cavity can be effectively limited, so that low temperature in the cold source cavity cannot be conducted into the taking and placing cavity through the reagent bottle; the cold air in the cold source cavity can better act on the reagent, and meanwhile the situation that low temperature in the cold source cavity is conducted into the taking and placing cavity, so that the internal temperature of the taking and placing cavity is low, and a user fetches the reagent and fetches the reagent is uncomfortable can be avoided.

Description

Technical Field

[0001] The present invention relates to lung cancer detection kit technology, and in particular to a lung cancer detection kit. Background Art

[0002] Lung cancer is one of the most serious diseases threatening human health. Early detection and diagnosis is a difficult task for medical staff. Lung cancer has few symptoms in the early stage, and most patients who seek medical treatment due to symptoms are in the middle and late stages. Currently, imaging examinations and pathological histological examinations are the main means of diagnosing lung cancer. Studies have shown that nearly 70% of lung cancer patients have missed the best treatment time when they seek medical treatment. Therefore, early detection and early diagnosis of lung cancer and improving the prognosis of lung cancer are still a major problem that needs to be solved urgently, and are also one of the important topics of current medical research.

[0003] The formation of tumors is a multi-factor, multi-stage process, which is the result of the combined effects of environmental factors and individual genetic factors. In recent years, molecular genetic studies from the perspective of oncogenes and tumor suppressor genes have shown that the occurrence and development of tumors is an accumulation process involving multiple gene changes and synergistic effects. As more and more oncogenes and tumor suppressor genes are recognized by humans, the clinical tumor gene detection technology is becoming more mature. At present, the most clinical lung cancer gene detection projects include eEF2, CEP350, EGFR, ALK, KRAS, HER2, BRAF, PI K3CA, AKT I, MEKI, NRAS and MET. Related studies have reported that human eukaryotic elongation factor 2 (eEF2) and human centrosomal protein 350kDa (CEP350) are two genes that are closely related to the occurrence and development of lung cancer. The combined detection of eEF2 and CEP350 antibodies in serum can improve the sensitivity and total coincidence rate of early detection, recurrence and metastasis of lung cancer.

[0004] At present, the reagents used to detect eEF2 and CEP350 antibodies are stored in different test kits, which need to be purchased separately. The content of eEF2 and CEP350 antibodies cannot be detected simultaneously on the same ELISA plate, and these test kits are basically paper-packaged, and foam boxes and ice packs are required for packaging during transportation. In addition, the standard products currently need to be dissolved by adding sample diluent before use, but due to the inaccuracy or imprecision of the pipette, the concentration of the standard products will be inaccurate, and finally the test results will be inaccurate. Therefore, it is an urgent problem to provide a test kit that can ensure the accuracy of the concentration of the standard products and has the reagents required for the experiment placed in a long-distance transportation.

[0005] The Chinese utility model patent with the announcement number CN207007855U discloses a lung cancer detection kit. The lung cancer detection kit uses vacuum insulation materials and cold preservation devices for the box body insulation part A and the box cover insulation part B, which contain reagents that need to be transported under low temperature conditions, to ensure that the reagents are always kept at a low temperature during transportation. The ELISA plate and the plastic film plate cover that do not need to be stored at low temperatures are placed in the normal temperature part A of the box body and the normal temperature part B of the box cover, which not only avoids the need for paper packaging kits currently sold on the market During transportation, foam boxes and ice packs are required for packaging, and low temperature and normal temperature environments can be achieved in one box; the standard product bottle is divided into an outer bottle body and an inner bottle body, the outer bottle body is filled with a specified volume of standard product diluent, and the inner bottle body is filled with a standard product, and the two are separated by a plastic film partition. When in use, the plastic film partition is opened with a film breaker, and a standard product solution of a predetermined concentration can be formed after dissolving and mixing. eEF2 and CEP350 can also be coated on the same ELISA plate at the same time, and eEF2 and CEP350 antibodies can be detected at the same time. The utility model can quickly and easily detect lung cancer, and has high sensitivity and accuracy.

[0006] The above application has solved the existing problems to a certain extent, but in the actual application process, there are still problems that need to be improved: when preparing for reagent testing, the reagents to be tested need to be mixed or other steps need to be performed, which may take a lot of time. During the use of the test kit in the above application, when the box lid is opened, the interior is in a completely open state, and the cold air inside will quickly diffuse into the external environment, causing the internal temperature to rise rapidly, which is not conducive to the preservation of post-processed reagents. In addition, the standard bottle is separated from the inner bottle body and the outer bottle body by a plastic film partition. When mixing the reagents, a membrane breaker is needed to break it open. During the operation of the membrane breaker, the membrane breaker head part will enter the inner part of the outer bottle body, causing more reagents to adhere to the surface, and when it is taken out, some reagents will be taken away, resulting in deviations in the amount of reagents in the medicine bottle, which reduces the accuracy of the final test results. Summary of the invention

[0007] The purpose of the present invention is to provide a kit for lung cancer detection to solve the above-mentioned deficiencies in the prior art.

[0008] In order to achieve the above-mentioned object, the present invention provides the following technical scheme: a test kit for lung cancer detection, comprising a box body and a sealed box cover arranged on the box body, the interior of the box body is divided into a normal temperature part and a refrigerated part, the top of the refrigerated part is provided with a take-in and put-out cavity, the bottom of the refrigerated part is provided with a cold source cavity, a cold source box is provided inside the cold source cavity, a cold source component is placed inside the cold source box, a plurality of placement grooves communicating with the cold source cavity are provided on the surface of the take-in and put cavity, a medicine bottle is placed inside the placement groove, a supporting cylinder corresponding to the placement groove is fixedly installed on the top of the inner wall of the cold source cavity, the side surface of the supporting cylinder is annularly provided with connecting grooves equidistantly, and a leakage prevention mechanism is provided inside the refrigerated part;

[0009] The anti-leakage mechanism is used to seal the placement slot after the medicine bottle is placed;

[0010] The medicine bottle includes an outer bottle body, the inner bottle body is fixedly connected to the interior of the outer bottle body, a stopper is provided at the bottle mouth of the outer bottle body, a liquid outlet groove is opened on the surface of the inner bottle body, and a sealing plate corresponding to the liquid outlet groove is fixedly connected to the side of the stopper close to the inner bottle body.

[0011] Furthermore, the anti-leakage mechanism includes an insulating sealing sleeve fixedly connected to the surface of the taking and placing cavity, the inner insulating sealing sleeve is arranged concentrically with the placement groove, the top of the inner wall of the sealed box cover is fixedly connected with an outer insulating sealing sleeve that is compatible with the inner insulating sealing sleeve, and an auxiliary component is arranged between the box body and the sealed box cover.

[0012] Furthermore, the auxiliary component includes a vertical groove opened inside the box body, a vertical plate is opened inside the vertical groove, two symmetrically arranged connecting protrusions are fixedly connected to the surface of the vertical plate, and a rotating groove adapted to the connecting protrusion is opened on the surface of the vertical plate. The connecting protrusion is rotatably connected to the inner side surface of the rotating groove, and the size value of the connecting protrusion is adapted to the rotating groove.

[0013] Furthermore, the two sides of the vertical plate are symmetrically fixedly connected to the positioning slide block, and the two sides of the inner wall of the vertical groove are symmetrically provided with positioning slide grooves, and the positioning slide block slides with the inner side surface of the positioning slide groove, and the movable travel distance value of the positioning slide block in the positioning slide groove is adapted to the length value of the outer insulation sealing sleeve.

[0014] Furthermore, four groups of locks are symmetrically installed in a rectangular shape between the box body and the sealed box cover, and the four groups of locks are symmetrically arranged on a side surface away from the normal temperature part and the refrigerated part, and the locks are used to fix the box body and the sealed box cover.

[0015] Furthermore, one side of the cold source cavity passes through the surface of the box body, and the end of the cold source box is fixedly connected with an outer sealing plate, and the outer sealing plate is fixedly connected to the box body by fastening bolts. The outer sealing plate can fully seal the opening of the cold source cavity, and a sealing and heat-insulating arrangement is provided between the outer sealing plate and the cold source cavity.

[0016] Furthermore, an auxiliary sealing mechanism is provided inside the supporting tube, and the auxiliary sealing mechanism includes a return spring fixedly connected to the bottom of the inner wall of the supporting tube, the top of the return spring is fixedly connected to the chassis, the top of the chassis is rotatably connected to the sealing tray, and a positioning assembly is provided inside the sealing tray.

[0017] Furthermore, the position and size of the sealing tray correspond to the placement groove. Under normal circumstances, the sealing tray can seal the bottom of the placement groove.

[0018] Furthermore, the positioning assembly includes limiting grooves equidistantly arranged on the inner wall of the supporting tube in the vertical direction, the inner side surface of the supporting tube is provided with a reset groove connected to one side of all the limiting grooves, the inner side surface of the sealing tray is provided with a movable groove, the inner side surface of the movable groove is fixedly connected with an extrusion spring, the end of the extrusion spring is fixedly connected with a limiting block, the bottom end of the limiting block close to one side of the limiting groove is provided with a guiding arc surface, and the bearing surface of the sealing tray is roughly arranged.

[0019] Furthermore, the position of the limiting groove corresponds to the limiting block and the guiding arc surface, and the size and specification of the reset groove are compatible with the limiting block.

[0020] Compared with the prior art, the lung cancer detection kit provided by the present invention has the following beneficial effects:

[0021] 1. The lung cancer detection kit, during the reagent transportation process, cools down and refrigerates the reagent inside the medicine bottle by directly contacting the cold air in the cold source cavity, and can effectively limit the leakage of the cold air in the cold source cavity, so that the low temperature inside the cold source cavity will not be transmitted to the loading and unloading cavity through the medicine bottle, so that the cold air in the cold source cavity can better act on the reagent, and at the same time, it can also avoid the low temperature in the cold source cavity being transmitted to the loading and unloading cavity, resulting in a low internal temperature, which will cause discomfort to the user when taking the reagent.

[0022] 2. The lung cancer detection kit seals the liquid outlet groove on the inner bottle body by a sealing plate, so that the reagents in the inner bottle body and the outer bottle body can be separated during transportation. During the test, the inner bottle body and the outer bottle body can be connected by turning the stopcock, thereby facilitating the mixed detection of the reagents.

[0023] 3. The lung cancer detection kit, through the use of an auxiliary sealing mechanism, can seal the placement slot where a single medicine bottle is stored when the medicine bottle is taken out for use, so that the cold air inside the cold source cavity will not overflow from the placement slot, thereby ensuring the normal refrigerated storage of other reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0025] Figure 1 A schematic diagram of the overall structure provided by an embodiment of the present invention;

[0026] Figure 2 A schematic diagram of the structure of the sealed box cover in an open state provided by an embodiment of the present invention;

[0027] Figure 3 A schematic diagram of the internal structure of a box provided by an embodiment of the present invention;

[0028] Figure 4 The embodiment of the present invention provides Figure 3 The enlarged structural diagram at A in the middle;

[0029] Figure 5 A schematic diagram of the support cylinder structure provided by an embodiment of the present invention;

[0030] Figure 6 A schematic diagram of a transverse cross-sectional structure of a support tube provided in an embodiment of the present invention;

[0031] Figure 7 A schematic diagram of the structure of a medicine bottle provided in an embodiment of the present invention.

[0032] Description of reference numerals:

[0033] 1. Box body; 2. Sealed box cover; 3. Normal temperature part; 4. Refrigeration part; 5. Take-in and put-out chamber; 6. Cold source chamber; 7. Placement slot; 8. Medicine bottle; 81. Outer bottle body; 82. Inner bottle body; 83. Plug; 84. Liquid outlet slot; 85. Sealing plate; 9. Anti-leakage mechanism; 91. Inner heat-insulating sealing sleeve; 92. Outer heat-insulating sealing sleeve; 93. Auxiliary components; 931. Vertical slot; 932. Vertical plate; 933. Connecting convex Block; 934, positioning slider; 935, positioning slide groove; 936, lock; 10, cold source box; 11, supporting tube; 12, connecting groove; 13, auxiliary sealing mechanism; 131, reset spring; 132, chassis; 133, sealing tray; 134, positioning assembly; 1341, limiting groove; 1342, reset groove; 1343, extrusion spring; 1344, limiting block; 1345, guide arc surface. DETAILED DESCRIPTION

[0034] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0035] Example:

[0036] See also Figure 1-Figure 7 A lung cancer detection kit comprises a box body 1 and a sealed box cover 2 arranged on the box body 1, the interior of the box body 1 is divided into a normal temperature part 3 and a refrigerated part 4, a take-in and put-out cavity 5 is provided at the top of the refrigerated part 4, a cold source cavity 6 is provided at the bottom of the refrigerated part 4, a cold source box 10 is provided inside the cold source cavity 6, a cold source component is placed inside the cold source box 10, a plurality of placement grooves 7 communicating with the cold source cavity 6 are provided on the surface of the take-in and put cavity 5, a medicine bottle 8 is placed inside the placement groove 7, a supporting cylinder 11 corresponding to the placement groove 7 is fixedly installed on the top of the inner wall of the cold source cavity 6, a side surface of the supporting cylinder 11 is provided with a circular ring-shaped connecting groove 12 equidistantly provided, and a leakage prevention mechanism 9 is provided inside the refrigerated part 4;

[0037] The anti-leakage mechanism 9 is used to seal the placement slot 7 after the medicine bottle 8 is placed;

[0038] The medicine bottle 8 includes an outer bottle body 81, the interior of the outer bottle body 81 is fixedly connected to an inner bottle body 82, a stopper 83 is provided at the bottle mouth of the outer bottle body 81, a liquid outlet groove 84 is opened on the surface of the inner bottle body 82, and a sealing plate 85 corresponding to the liquid outlet groove 84 is fixedly connected to the side of the stopper 83 close to the inner bottle body 82.

[0039] Specifically, several reagent bottles 8 are respectively filled with eEF2 protein, CEP350 protein, HRP-sheep anti-human IgG antibody, eEF2 antibody, CEP350 antibody, washing buffer, specimen diluent, substrate A, substrate B, and stop solution;

[0040] Furthermore, when the standard solution eEF2 antibody or CEP350 antibody standard solution is mixed with the specimen diluent to prepare the standard dilution solution, the stopcock 83 is manually turned, and the rotation of the stopcock 83 drives the sealing plate 85 to rotate. The sealing plate 85 rotates and gradually shifts from the liquid outlet groove 84, so that the liquid outlet groove 84 connects the outer bottle body 81 with the inner bottle body 82. At this time, the liquid in the inner bottle body 82 and the liquid inside the outer bottle body 81 can be fully mixed, and there will be no loss of any liquid.

[0041] In this embodiment, the anti-leakage mechanism 9 includes an inner insulating sealing sleeve 91 fixedly connected to the surface of the taking and placing cavity 5, the inner insulating sealing sleeve 91 is arranged concentrically with the placement groove 7, the top of the inner wall of the sealed box cover 2 is fixedly connected with an outer insulating sealing sleeve 92 that is compatible with the inner insulating sealing sleeve 91, and an auxiliary component 93 is arranged between the box body 1 and the sealed box cover 2.

[0042] Specifically, the outer heat-insulating sealing sleeve 92 can be sleeved on the outside of the inner heat-insulating sealing sleeve 91, and the contact sealing performance between the two is good.

[0043] In this embodiment, the auxiliary component 93 includes a vertical groove 931 opened inside the box body 1, a vertical plate 932 is opened inside the vertical groove 931, two symmetrically arranged connecting protrusions 933 are fixedly connected to the surface of the vertical plate 932, and a rotating groove matched with the connecting protrusion 933 is opened on the surface of the vertical plate 932. The connecting protrusion 933 is rotatably connected to the inner side surface of the rotating groove, and the size value of the connecting protrusion 933 is matched with the rotating groove.

[0044] Specifically, during the synchronous rotation of the sealing box cover 2 , the thickness direction portion of the connecting protrusion 933 can be rotated to be immersed in the rotating groove, so that the connecting protrusion 933 can be synchronously moved downward with the vertical plate 932 into the vertical groove 931 .

[0045] Furthermore, the two sides of the vertical plate 932 are symmetrically fixedly connected to the positioning slide 934, and the two sides of the inner wall of the vertical groove 931 are symmetrically provided with positioning slide grooves 935. The positioning slide slider 934 slides with the inner side surface of the positioning slide groove 935, and the movable travel distance value of the positioning slide slider 934 in the positioning slide groove 935 is adapted to the length value of the outer insulation sealing sleeve 92.

[0046] Furthermore, during the opening process, the sealed box cover 2 can first move in the vertical direction so that the outer insulating sealing sleeve 92 can be separated from the inner insulating sealing sleeve 91, thereby exposing the end of the medicine bottle 8 for easy removal and use.

[0047] In this embodiment, four sets of locks 936 are symmetrically installed in a rectangular shape between the box body 1 and the sealed box cover 2, and the four sets of locks 936 are symmetrically arranged on the side surface away from the normal temperature part 3 and the refrigeration part 4. The locks 936 are used to fix the box body 1 and the sealed box cover 2.

[0048] Specifically, the lock buckle 936 includes two parts, a buckle and a locking buckle. It is a common lock buckle 936 used on bags in the market, wherein the buckle is fixedly connected to the sealed box cover 2, and the locking buckle is installed on the box body 1 at a position corresponding to the buckle, so that the two can cooperate with each other to connect and fix the box body 1 and the sealed box cover 2.

[0049] In this embodiment, one side of the cold source cavity 6 passes through the surface of the box body 1, and the end of the cold source box 10 is fixedly connected with an outer sealing plate, which is fixedly connected to the box body 1 by fastening bolts. The outer sealing plate can fully seal the cavity opening of the cold source cavity 6, and a sealing and heat-insulating arrangement is provided between the outer sealing plate and the cold source cavity 6.

[0050] Specifically, after the cold source box 10 is placed inside the cold source cavity 6, the cold source component placed inside the cold source box 10 releases cold air to cool down the medicine bottle 8;

[0051] Furthermore, when selecting a cold source, ice cubes or other cold sources that can be used to assist in cooling can be directly selected;

[0052] Furthermore, the outer sealing plate and the side of the box body 1 are provided with corresponding thread grooves, and the cold source box 10 can be stably fixed to the box body 1 by fastening bolts and threaded connection with the thread grooves, so that it can maintain a stable state during operation;

[0053] It should be further explained that the cold source box 10 and the housing 1 are in a detachable connection state. In addition to being connected by fastening bolts, the two may also be connected by connecting parts such as snap fasteners that can achieve detachable connection.

[0054] In this embodiment, an auxiliary sealing mechanism 13 is provided inside the supporting tube 11, and the auxiliary sealing mechanism 13 includes a reset spring 131 fixedly connected to the bottom of the inner wall of the supporting tube 11, and the top of the reset spring 131 is fixedly connected to a chassis 132, and the top of the chassis 132 is rotatably connected to a sealing tray 133, and a positioning assembly 134 is provided inside the sealing tray 133.

[0055] Specifically, the bottom plate 132 and the sealing tray 133 are both slidably connected to the inner side surface of the supporting tube 11 .

[0056] Furthermore, the position and size of the sealing tray 133 correspond to the placement groove 7 . Under normal circumstances, the sealing tray 133 can seal the bottom of the placement groove 7 .

[0057] In this embodiment, the positioning assembly 134 includes limiting grooves 1341 equidistantly arranged on the inner wall of the supporting tube 11 in the vertical direction, the inner side surface of the supporting tube 11 is provided with a reset groove 1342 connected to one side of all the limiting grooves 1341, the inner side surface of the sealing tray 133 is provided with a movable groove, the inner side surface of the movable groove is fixedly connected with an extrusion spring 1343, the end of the extrusion spring 1343 is fixedly connected with a limiting block 1344, the bottom end of the limiting block 1344 close to the limiting groove 1341 is provided with a guide arc surface 1345, and the bearing surface of the sealing tray 133 is roughly arranged.

[0058] Specifically, the relative sliding friction resistance value between the contact surface of the medicine bottle 8 and the sealing tray 133 is greater than the relative sliding friction resistance value between the contact surface of the limiting block 1344 and the limiting groove 1341, so that when the medicine bottle 8 needs to be taken out, the sealing tray 133 can be rotated synchronously by rotating the medicine bottle 8.

[0059] In this embodiment, the position of the limiting groove 1341 corresponds to the limiting block 1344 and the guiding arc surface 1345 , and the size and specification of the reset groove 1342 are compatible with the limiting block 1344 .

[0060] Specifically, when the limiting block 1344 is subjected to downward pressure, under the guiding action of the guiding arc surface 1345, the limiting block 1344 is subjected to a component force moving toward the inside of the movable groove, so that the limiting block 1344 can move downward normally;

[0061] Furthermore, under normal working conditions, the end of the limiting block 1344 away from the guiding arc surface 1345 cooperates with the limiting groove 1341 to limit the resetting of the limiting block 1344 .

[0062] It should be noted that the components involving thermal insulation and sealing related functions in this application can be made of thermal insulation materials to have better thermal insulation effects, or a thermal insulation sealing layer can be set on the outside of the above-mentioned components to improve the thermal insulation performance of each component.

[0063] When placing the medicine bottle 8, place the medicine bottle 8 in the placement groove 7 and press it down, so that the sealing tray 133 moves downward and drives the bottom plate 132 to move downward to compress the return spring 131. When the medicine bottle 8 is placed, at this time, under the cooperation of the limiting block 1344 and the limiting groove 1341, the return of the sealing tray 133 can be restricted, so that the medicine bottle 8 can be in a stable placement state. When the medicine bottle 8 is placed, the operator rotates the sealing box cover 2 to a state parallel to the upper surface of the box body 1, and then moves the sealing box cover 2 downward, so that the outer heat insulating sealing sleeve 92 can move downward and be connected to the outside of the inner heat insulating sealing sleeve 91, so that the medicine bottle 8 can be separated from the loading and unloading cavity 5, which can effectively prevent the leakage of cold air. When the sealing box cover 2 is closed, the sealing box cover 2 is connected and fixed to the box body 1 through the lock buckle 936;

[0064] During the transportation process, cold air is output through the cold source component in the cold source box 10 and directly acts on the medicine bottle 8 through the connecting groove 12, so that the preservation effect of the reagent in the medicine bottle 8 is better.

[0065] During use, when the medicine bottle 8 needs to be taken, the operator opens the lock 936 and moves the sealed box cover 2 upward. The movement of the sealed box cover 2 drives the positioning slider 934 to slide along the inside of the positioning slide groove 935. When the positioning slider 934 moves to the extreme position, the outer heat insulating sealing sleeve 92 is separated from the inner heat insulating sealing sleeve 91. At this time, the operator can rotate the sealed box cover 2 to open it.

[0066] After the sealed box cover 2 is opened, the operator manually rotates the medicine bottle 8, and the rotation of the medicine bottle 8 drives the sealing tray 133 to rotate, and the rotation of the sealing tray 133 drives the limiting block 1344 to rotate, so that the limiting block 1344 can rotate into the reset groove 1342, so that the upward movement of the limiting block 1344 is no longer restricted. At this time, under the action of the rebound force of the extrusion spring 1343, the sealing tray 133 moves upward and pushes out the medicine bottle 8. After the medicine bottle 8 is taken out, the sealing tray 133 moves up to the extreme position, and the connection between the placement groove 7 and the connecting groove 12 is closed, so that the cold air in the cold source cavity 6 will not leak into the external environment.

[0067] The above description is only by way of illustration of certain exemplary embodiments of the present invention. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A lung cancer detection kit, comprising a box body (1) and a sealed box cover (2) arranged on the box body (1), wherein the interior of the box body (1) is divided into a normal temperature section (3) and a refrigerated section (4), characterized in that: The top of the refrigerating part (4) is provided with a taking-in and putting-out cavity (5), the bottom of the refrigerating part (4) is provided with a cold source cavity (6), a cold source box (10) is arranged inside the cold source cavity (6), a cold source component is placed inside the cold source box (10), a surface of the taking-in and putting-out cavity (5) is provided with a plurality of placement grooves (7) communicating with the cold source cavity (6), medicine bottles (8) are placed inside the placement grooves (7), a supporting cylinder (11) corresponding to the placement grooves (7) is fixedly mounted on the top of the inner wall of the cold source cavity (6), the side surface of the supporting cylinder (11) is provided with communicating grooves (12) in annular shape and equidistantly, and a leakage prevention mechanism (9) is arranged inside the refrigerating part (4); The anti-leakage mechanism (9) is used to seal the placement groove (7) after the medicine bottle (8) is placed therein; The medicine bottle (8) comprises an outer bottle body (81), the inner part of the outer bottle body (81) is fixedly connected to an inner bottle body (82), a stopper (83) is arranged at the bottle mouth of the outer bottle body (81), a liquid outlet groove (84) is opened on the surface of the inner bottle body (82), and a sealing plate (85) corresponding to the liquid outlet groove (84) is fixedly connected to the side of the stopper (83) close to the inner bottle body (82).

2. A kit for lung cancer detection according to claim 1, characterized in that: The anti-leakage mechanism (9) comprises an inner heat-insulating sealing sleeve (91) fixedly connected to the surface of the loading and unloading chamber (5), the inner heat-insulating sealing sleeve (91) being arranged concentrically with the placement groove (7), an outer heat-insulating sealing sleeve (92) matching the inner heat-insulating sealing sleeve (91) being fixedly connected to the top of the inner wall of the sealed box cover (2), and an auxiliary component (93) being arranged between the box body (1) and the sealed box cover (2).

3. A kit for lung cancer detection according to claim 2, characterized in that: The auxiliary component (93) comprises a vertical groove (931) provided inside the box body (1), a vertical plate (932) provided inside the vertical groove (931), two symmetrically arranged connecting protrusions (933) fixedly connected to the surface of the vertical plate (932), a rotating groove matched with the connecting protrusions (933) provided on the surface of the vertical plate (932), the connecting protrusions (933) being rotatably connected to the inner side surface of the rotating groove, and the size value of the connecting protrusions (933) being matched with the rotating groove.

4. A kit for lung cancer detection according to claim 3, characterized in that: The two sides of the vertical plate (932) are symmetrically fixedly connected to the positioning slide block (934), and the two sides of the inner wall of the vertical groove (931) are symmetrically provided with positioning slide grooves (935). The positioning slide block (934) slides with the inner side surface of the positioning slide groove (935), and the movable travel distance value of the positioning slide block (934) in the positioning slide groove (935) is adapted to the length value of the outer heat insulation sealing sleeve (92).

5. A kit for lung cancer detection according to claim 4, characterized in that: Four groups of lock buckles (936) are symmetrically installed in a rectangular shape between the box body (1) and the sealed box cover (2), and the four groups of lock buckles (936) are symmetrically arranged on a side surface away from each other between the normal temperature part (3) and the refrigeration part (4), and the lock buckles (936) are used to fix the box body (1) and the sealed box cover (2).

6. A kit for lung cancer detection according to claim 5, characterized in that: One side of the cold source cavity (6) passes through the surface of the box body (1); an outer sealing plate is fixedly connected to the end of the cold source box (10); the outer sealing plate is fixedly connected to the box body (1) by means of fastening bolts; the outer sealing plate can fully seal the cavity opening of the cold source cavity (6); a sealing and heat-insulating arrangement is provided between the outer sealing plate and the cold source cavity (6).

7. A kit for lung cancer detection according to claim 6, characterized in that: An auxiliary sealing mechanism (13) is arranged inside the supporting cylinder (11), and the auxiliary sealing mechanism (13) comprises a return spring (131) fixedly connected to the bottom of the inner wall of the supporting cylinder (11), the top of the return spring (131) is fixedly connected to a base plate (132), the top of the base plate (132) is rotatably connected to a sealing tray (133), and a positioning assembly (134) is arranged inside the sealing tray (133).

8. A kit for lung cancer detection according to claim 7, characterized in that: The position and size of the sealing tray (133) correspond to the placement groove (7). Under normal circumstances, the sealing tray (133) can seal the bottom of the placement groove (7).

9. A kit for lung cancer detection according to claim 8, characterized in that: The positioning assembly (134) comprises limiting grooves (1341) which are equidistantly arranged on the inner wall of the supporting tube (11) in the vertical direction; the inner side surface of the supporting tube (11) is provided with a reset groove (1342) which is connected to one side of all the limiting grooves (1341); the inner side surface of the sealing tray (133) is provided with a movable groove; the inner side surface of the movable groove is fixedly connected with an extrusion spring (1343); the end of the extrusion spring (1343) is fixedly connected with a limiting block (1344); the bottom end of the limiting block (1344) close to one side of the limiting groove (1341) is provided with a guiding arc surface (1345); and the bearing surface of the sealing tray (133) is roughly arranged.

10. A kit for lung cancer detection according to claim 9, characterized in that: Place The position of the limiting groove (1341) corresponds to the limiting block (1344) and the guiding arc surface (1345). The size and specifications of the reset groove (1342) are compatible with the limiting block (1344).

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