Sample sampling method

Abstract

The invention discloses a sample sampling method, which comprises a sampling step and a cleaning step, and the sampling step comprises the following contents: firstly, extracting a certain volume of cleaning liquid by using a sample adding pump, so that an upper cleaning liquid column is formed in a sample adding needle; extracting the isolation medium by using a sample adding pump to form a section of isolation column at the bottom of the upper cleaning liquid column; finally, a certain volume of sample liquid is extracted to form a lower sample column; the cleaning step comprises the following contents: injecting a lower sample column into a reaction container by using a sample adding pump, moving a sample adding needle to a cleaning station, injecting an upper cleaning liquid column into the cleaning station by using the sample adding pump, and cleaning the sample needle by using the upper cleaning liquid column. According to the invention, the mode of pre-pumping the cleaning fluid and isolating the cleaning fluid by using the isolation column is adopted for sampling, so that accurate control of the amount of the cleaning fluid can be realized, an additional flushing pump and a corresponding pipeline are not needed, the system is simplified, and the installation requirement and the system cost of the system are reduced.

Description

Technical Field

[0001] The present invention relates to the field of sample processing of in vitro diagnostic equipment, and in particular to a sample sampling method. Background Art

[0002] In the field of in vitro diagnosis, in vitro diagnostic equipment is often used to perform a series of processing on samples, and finally test and give test results. Among them, the accuracy and stability of sample sampling directly affect the reliability of the test results. According to statistical analysis, the biggest reason for the failure of in vitro diagnostic tests is caused by abnormalities in the sample addition system. The sampling method of existing in vitro diagnostic equipment is generally to use a plunger pump for sampling and injection, and then use a flushing pump to internally flush the plunger pump and the sample addition needle, and use an external flushing pump and a washing station to externally clean the sample addition needle. During the internal cleaning process, in order to meet the system flow requirements, the pump pressure of the external flushing pump is often relatively high, and high requirements are placed on the pipeline and valves; the flushing pump, plunger pump and sample addition needle are connected in sequence, the system is relatively complex, the cost is high, and the amount of washing solution cannot be accurately controlled; the pipeline flow resistance of the existing system is large, resulting in a pressure drop from the flushing pump to the sample addition needle. In order to ensure the pressure transmission efficiency, the existing technology has high requirements for the system layout. Summary of the invention

[0003] In view of this, the present invention proposes a sample sampling method, which adopts the method of pre-extracting cleaning liquid and isolating the cleaning liquid and the sample with an isolation column, which can not only achieve accurate control of the amount of cleaning liquid, but also simplify the system.

[0004] To achieve the above object, the present invention adopts the following technical solutions: The sample sampling method of the present invention comprises a sampling step and a cleaning step, wherein the sampling step comprises the following contents: firstly, a certain volume of cleaning liquid is extracted by a sampling pump to form an upper cleaning liquid column in a sampling needle; then, an isolation medium is extracted by a sampling pump to form an isolation column at the bottom of the upper cleaning liquid column; finally, a certain volume of sample liquid is extracted to form a lower sample column; and the cleaning step comprises the following contents: the lower sample column is injected into a reaction container by a sampling pump, an upper cleaning liquid column is injected into the washing station after the sampling needle is moved to the washing station by a sampling pump, and the sample needle is cleaned by the upper cleaning liquid column.

[0005] The beneficial effect is that the present invention adopts the method of pre-pumping the cleaning liquid and isolating it with an isolation column to sample, which can achieve precise control of the amount of cleaning liquid used, and does not require an additional flushing pump and corresponding pipelines, thereby simplifying the system, reducing the system installation requirements and system costs, and is of great significance.

[0006] Preferably, the sample pump is a reciprocating pump, and a pressure detection module is arranged on the pipeline between the sample pump and the sample needle, the signal output end of the pressure detection module is connected to the signal input end of the control system of the instrument, the control input end of the sample pump is connected to the control output end of the control system, and the signal output end of the control system is connected to the signal input end of the alarm module. The present invention adopts the method of pre-pumping cleaning liquid, and only needs to connect the sample pump and the sample needle with a pipeline, which can realize self-cleaning while realizing sampling, without the need for additional flushing pipelines and flushing pumps, simplifying the system and reducing costs. In operation, the pressure detection module outputs the detected pressure signal to the control system, and the control system processes the received signal. When the pressure is too high or too low, the alarm module alarms, which can be displayed on the operating screen of the instrument, which is conducive to quickly determining the fault location.

[0007] Preferably, the isolation medium is air or sample liquid, and the isolation column is an air isolation column or a sample liquid isolation column. When the isolation medium is air, the air separates the cleaning liquid and the sample liquid to prevent the cleaning liquid from diluting the sample liquid; when the isolation medium is sample liquid, the sample liquid isolation column can also prevent the pre-extracted cleaning liquid from being separated from the lower sample column section. The present invention achieves quantitative cleaning by pre-extraction.

[0008] Preferably, the inner diameter of the sample injection needle is D , the volume of the isolation column ≥ πD 3 / 2. When determining the volume of the isolation column, a sphere with the same inner diameter as the sample needle is used as a reference. The volume of the isolation column is 3 times or more of the volume of the sphere to ensure sufficient isolation space to meet the isolation requirements of the sample and the cleaning solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] Figure 1 It is a pipeline schematic diagram of the present invention.

[0010] Figure 2 This is a status diagram of the upper cleaning liquid column, isolation column and lower sample column in the sampling needle during sampling.

[0011] Figure 3 It is a circuit principle block diagram of the present invention. DETAILED DESCRIPTION

[0012] The following is a detailed description of an embodiment of the present invention in conjunction with the accompanying drawings. This embodiment is implemented on the premise of the technical solution of the present invention, and a detailed implementation method and a specific operation process are given, but the protection scope of the present invention is not limited to the following embodiment.

[0013] like Figure 1-3As shown, the present invention proposes a sample sampling method, including a sampling step and a washing step, wherein the sampling step includes the following contents: moving the sample needle 1 to the washing station 2, using the sample pump P1 to extract a certain volume of washing liquid, and forming an upper washing liquid column M in the sample needle 1; raising the sample needle 1 to a certain height, using the sample pump P1 to extract an isolation medium to form an isolation column Q inside the sample needle; moving the sample pump P1 to the sample to extract a certain volume of sample to form a lower sample column N, the upper washing liquid column M, the isolation column Q and the lower sample column N are spaced from top to bottom in the sample needle 1, and the middle isolation column Q isolates the upper washing liquid column M from the lower sample column N to avoid contact between the washing liquid and the sample to cause sample dilution, etc.; The cleaning step includes the following: moving the sample needle 1 to the reaction container, injecting the lower sample column N of the sample needle 1 into the reaction container by using the sample pump P1; then moving the sample needle 1 to the washing station 2, injecting the upper cleaning liquid column M into the washing station 2 by using the sample pump P1, and then using the upper cleaning liquid column M to clean the sample needle; During external cleaning, the external cleaning pump P2 is used to pump the cleaning liquid to the washing station 2 to clean the outer wall of the sample injection needle 1, and the waste liquid pump P3 is used to pump away the cleaning waste liquid in the washing station 2. Figure 1 ; During actual installation, the sample pump P1 is a reciprocating pump, preferably a plunger reciprocating pump (i.e., a plunger pump), and a pressure detection module (a pressure sensor may be used) is provided on the pipeline between the sample pump P1 and the sample needle 1, and the signal output end of the pressure detection module is connected to the signal input end of the control system of the instrument, and the control input ends of the sample pump P1, the waste liquid pump P3, and the external washing pump P2 are connected to the control output end of the control system, and the signal output end of the control system is connected to the signal input end of the alarm module (such as an error sound prompt, which can also be displayed on the instrument's operating screen). The sampling method of the present invention does not require any additional internal cleaning pipelines, simplifies the internal cleaning system of the sample needle 1, and reduces costs; during operation, the pressure detection module outputs the detected pressure signal to the control system, and the control system processes the received signal. When the pressure is too high or too low, the alarm module alarms, which is conducive to quickly determining the fault location and performing repairs.

[0014] The isolation medium of the present invention can be air or sample liquid, and the isolation column can be an air isolation column or a sample liquid isolation column. The isolation prevents the cleaning liquid from contacting the lower sample column, thereby preventing the sample in the lower sample column from being diluted. In the field of in vitro diagnosis, commonly used cleaning fluids include purified water, BF cleaning fluid, etc. The amount of cleaning fluid pre-pumped during actual sampling can be determined according to the type of cleaning fluid and the requirements of the test item to ensure that the internal cleaning effect of the sample injection needle 1 can meet actual needs. Since the sample injection needle 1 often has a large length and volume, when the isolation column Q is an air isolation column, the upper cleaning fluid column M, the isolation column Q and the lower sample column N in the sample injection needle 1 can be Figure 2Any state of a and b.

[0015] The present invention uses a sphere with the same inner diameter as the sample needle 1 as a reference, and the volume of the isolation column Q is 3 times or more of the volume of the sphere to meet the isolation requirements of the sample and the cleaning solution. The inner diameter of the sample needle 1 is D, and the volume of the sphere corresponding to the inner diameter is πD 3 / 6, the volume of the isolation column Q ≥ πD 3 / 2.

[0016] The present invention adopts the method of pre-pumping cleaning liquid and isolating it with an isolation column to take samples. During internal cleaning, the sampling pump P1 injects the pre-pumped cleaning liquid into the washing station 2, that is, the pre-pumped cleaning liquid is used to achieve internal cleaning of the sampling needle, thereby achieving precise control of the amount of cleaning liquid used, and no additional flushing pump and corresponding pipelines are required, thereby simplifying the system, which not only reduces the installation requirements of the system on the instrument, but also reduces the system cost.

[0017] Finally, it should be emphasized that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions recorded in the aforementioned embodiments without creative work, or replace some of the technical features therein with equivalents. Therefore, any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A sample sampling method, characterized in that: The method comprises a sampling step and a cleaning step, wherein the sampling step comprises the following contents: firstly, a certain volume of cleaning liquid is extracted by a sampling pump to form an upper cleaning liquid column in the sampling needle; then, an isolation medium is extracted by a sampling pump to form an isolation column at the bottom of the upper cleaning liquid column; finally, a certain volume of sample liquid is extracted to form a lower sample column; and the cleaning step comprises the following contents: The lower sample column is injected into the reaction container by using the sample pump, and the upper cleaning liquid column is injected into the washing station after the sample needle is moved to the washing station by using the sample pump, and the sample needle is cleaned by using the upper cleaning liquid column.

2. The sample sampling method according to claim 1, characterized in that: The sample adding pump is a reciprocating pump, and a pressure detection module is arranged on the pipeline between the sample adding pump and the sample adding needle. The signal output end of the pressure detection module is connected to the signal input end of the control system of the instrument, the control input end of the sample adding pump is connected to the control output end of the control system, and the signal output end of the control system is connected to the signal input end of the alarm module.

3. The sample sampling method according to claim 1, characterized in that: The isolation medium is air or sample liquid, and the isolation column is an air isolation column or a sample liquid isolation column.

4. The sample sampling method according to claim 1, characterized in that: The inner diameter of the sample injection needle is D, and the volume of the isolation column is ≥ πD 3 / 2.

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