Instrument for automatic detection of electrochemical sensor

Abstract

The invention relates to the field of in-vitro diagnosis, in particular to an automatic detection instrument for an electrochemical sensor, which comprises a vertical electric sliding table provided with a sensor kit fixing sliding block, a first XZ combined electric sliding table provided with an electrode fixing sliding block, an electrode cleaning device and a second XZ combined electric sliding table provided with a liquid suction head fixing sliding block, the horizontal electric sliding table is provided with an injector push rod fixing sliding block and an injector body fixing block, and the injector is provided with a three-way valve; the invention provides an instrument for automatic detection of an electrochemical sensor, which can realize sampling tube taking and placing, electrode cleaning, rapid sample injection and sampling detection, avoid cross contamination, improve the detection accuracy, facilitate sample feeding and result output, and is simple to operate and high in automation degree.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnosis, and in particular to an instrument for automatic detection of electrochemical sensors. Background Art

[0002] The detection of target molecules or analytes in biological samples is crucial for diagnostic monitoring of health and disease. The key requirements for analyte detection are specificity and sensitivity, especially when the target molecule or analyte is present in a biological sample at a limited amount or concentration. Previous approaches involved the use of monoclonal antibodies that specifically bind to the analyte. This type of diagnostic approach is well known and widely used in the enzyme-linked immunosorbent sandwich assay (ELISA) format, which is the gold standard for detecting specific analytes in complex biological samples.

[0003] In the past three decades, electrochemical sensors have also become a practical alternative to complex and expensive analytical instruments used in healthcare, agriculture, and environmental monitoring. Among the several currently used detection technologies such as optical, acoustic, and piezoelectric, electrochemical detection instruments have great advantages of simple structure, low cost, high sensitivity, and easy integration. Electrochemical blood glucose sensors are the most commercially successful biosensors, accounting for nearly 90% of the global biosensor market.

[0004] However, in the prior art, there is no electrochemical sensor with a high degree of automation and multi-index measurement for medical clinical use. Summary of the invention

[0005] The purpose of the present invention is to provide an instrument for automated detection of electrochemical sensors to solve the problems of inconvenient clinical detection and low automation in the prior art.

[0006] To achieve this purpose, the technical solution of the present invention is summarized as follows: An instrument for automated detection of electrochemical sensors, comprising a vertical electric slide with a sensor reagent kit fixed slider, a first XZ combined electric slide with an electrode fixed slider, an electrode cleaning device, a second XZ combined electric slide with a pipette head fixed slider, a horizontal electric slide with a syringe push rod fixed slider and a syringe body fixed block, a syringe with a three-way valve, a first pipeline, a second pipeline, a barcode recognition device and a control system.

[0007] Furthermore, the sensor kit fixing slider is fixedly connected to a tray provided with a plurality of sensor kit slots, the sensor kit slots are provided with single-portion kits, the electrode fixing slider is fixedly connected to an electrochemical dual electrode, the pipette head fixing slider is fixedly connected to the pipette head connector, the syringe body fixing block is fixedly connected to the syringe body, the syringe push rod fixing slider is fixedly connected to the syringe push rod, and the three-way valve is connected to the first channel and the second channel of the pipette head connector respectively through the first pipeline and the second pipeline.

[0008] Furthermore, the first XZ combined electric slide is arranged in parallel with the second XZ combined electric slide, and the vertical electric slide is arranged vertically with the first XZ combined electric slide.

[0009] Furthermore, the electrode cleaning device comprises a cleaning tank, a cleaning liquid bottle, a waste liquid bottle and a diaphragm pump, the cleaning tank is provided with an electrode channel, a first cleaning liquid channel and a second cleaning liquid channel, the first cleaning liquid channel and the second cleaning liquid channel are connected to the electrode channel, the other ends of the first cleaning liquid channel and the second cleaning liquid channel are connected to the cleaning liquid bottle through the diaphragm pump, and the lower end of the electrode channel is connected to the waste liquid bottle.

[0010] Furthermore, the pipette head connector also includes a first sleeve arranged outside the first channel, a second sleeve arranged outside the second channel, and an electric push rod, the first sleeve is coaxial with the first channel and movably connected, the second sleeve is coaxial with the second channel and movably connected, and the moving end of the electric push rod is fixedly connected to the first sleeve and the second sleeve.

[0011] Furthermore, the syringe is connected to the three-way valve via a Luer connector.

[0012] Furthermore, the first pipeline and the second pipeline are made of silicone.

[0013] Furthermore, the control system is connected to the vertical electric slide, the first XZ combined electric slide, the second XZ combined electric slide, the horizontal electric slide, the electrochemical double electrode, the diaphragm pump, the electric push rod and the barcode recognition device through cables.

[0014] Advantages of the present invention: The present invention provides an instrument for automated detection of electrochemical sensors; the sleeve and push rod combination structure can realize the replacement of the suction needle to avoid cross contamination; the dual-channel liquid pipette head connector effectively improves the efficiency of liquid addition and pipetting; the double XZ combined electric slide and the vertical electric slide combination structure can realize rapid sampling and sampling detection, and in conjunction with the barcode recognition device, sample feeding and result output can be realized, with simple operation and high degree of automation; the electrode cleaning device can realize electrode cleaning, effectively avoid cross contamination, and improve the accuracy of detection. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of the internal structure of an instrument for automated detection of electrochemical sensors;

[0016] Figure 2 A schematic diagram of the external structure (partial cross-section) of an instrument for automated detection of electrochemical sensors;

[0017] Figure 3 It is a structural schematic diagram of a second XZ combined slide table of an instrument for automatic detection of electrochemical sensors;

[0018] Figure 4 It is a schematic diagram of the structure of a single-person test kit;

[0019] Figure 5 A partial cross-sectional view of a cleaning tank of an instrument for automated detection of electrochemical sensors;

[0020] Figure 6 The present invention is a control system flow chart of an instrument for automated detection of electrochemical sensors.

[0021] In the figure:

[0022] 1. Vertical electric slide, 11. Sensor reagent kit fixing slider, 12. Tray, 13. Sensor reagent kit slot, 14. Single-dose reagent kit, 141. Sample position, 142. Electrolyte position, 143. Sample aspiration needle position, 1431. First sample aspiration needle, 1432. Second sample aspiration needle, 144. Electrolyte aspiration needle position, 1441. First electrolyte aspiration needle, 1442. Second electrolyte aspiration needle, 145. Electrochemical sensor position; 2. First XZ combined electric slide, 21. Electrode fixing slider, 22. Electrochemical double electrode; 3. Electrode cleaning device, 31. Cleaning tank, 311. Electrode channel, 312. First cleaning liquid channel, 313. Second cleaning liquid channel, 32 , cleaning liquid bottle, 33, waste liquid bottle, 34, diaphragm pump; 4, second XZ combined electric slide, 41, pipette head fixed slider, 42, pipette head connector, 421, first channel, 422, second channel, 423, first sleeve, 424, second sleeve, 425, electric push rod; 5, horizontal electric slide, 51, syringe push rod fixed slider, 52, syringe body fixed block; 6, syringe, 61, three-way valve, 62, body, 63, push rod; 71, first pipeline, 72, second pipeline; 8, bar code recognition device; 9, control system, 91, printer, 92, display, 93, control button; 10, housing, 101, sample inlet, 102, liquid inlet. DETAILED DESCRIPTION

[0023] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It is also necessary to explain that, for ease of description, only the parts related to the present invention are shown in the accompanying drawings, rather than all.

[0024] Some directional words are defined in the present invention. Unless otherwise specified, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used to facilitate understanding and therefore do not constitute a limitation on the scope of protection of the present invention.

[0025] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0026] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0027] like Figure 1~Figure 5 As shown, an instrument for automated detection of electrochemical sensors includes a vertical electric slide 1 provided with a sensor kit fixing slider 11, a first XZ combined electric slide 2 provided with an electrode fixing slider 21, an electrode cleaning device 3, a second XZ combined electric slide 4 provided with a pipette head fixing slider 41, a horizontal electric slide 5 provided with a syringe push rod fixing slider 51 and a syringe body fixing block 52, a syringe 6 provided with a three-way valve 61, a first pipeline 71, a second pipeline 72, a barcode recognition device 8, a control system 9 and a housing 10.

[0028] The sensor reagent kit fixing slider 11 is fixedly connected to a tray 12 provided with eight sensor reagent kit slots 13 , and the sensor reagent kit slots 13 are provided with single-person reagent kits 14 .

[0029] The electrode fixing slider 21 is fixedly connected with an electrochemical double electrode 22 , which is a pair of commercially available platinum electrodes.

[0030] The electrode cleaning device 3 comprises a cleaning tank 31, a cleaning liquid bottle 32, a waste liquid bottle 33 and a diaphragm pump 34. The cleaning tank 31 is provided with an electrode channel 311, a first cleaning liquid channel 312 and a second cleaning liquid channel 313. The first cleaning liquid channel 312 and the second cleaning liquid channel 313 are connected to the electrode channel 311. The upper ends of the first cleaning liquid channel 312 and the second cleaning liquid channel 313 are connected to the outlet end of the diaphragm pump 34 through a silicone tube, and the inlet end of the diaphragm pump 34 is connected to the cleaning liquid bottle 32. The lower end of the electrode channel 311 is connected to the waste liquid bottle 33. The diaphragm pump 34 pumps the cleaning liquid in the cleaning liquid bottle 32 and sprays the cleaning liquid through the first cleaning liquid channel 312 and the second cleaning liquid channel 313 to clean the electrochemical double electrode 22. The waste liquid after cleaning flows into the waste liquid bottle 33 for collection.

[0031] The pipette head fixed slider 41 is fixedly connected to the pipette head connector 42, and the pipette head connector 42 also includes a first sleeve 423 arranged outside the first channel 421, a second sleeve 424 arranged outside the second channel 422, and an electric push rod 425. The first sleeve 423 is coaxial with the first channel 421 and movably connected, the second sleeve 424 is coaxial with the second channel 422 and movably connected, the fixed end of the electric push rod 425 is fixedly connected to the pipette head connector 42, and the movable end of the electric push rod 425 is fixedly connected to the first sleeve 423 and the second sleeve 424.

[0032] The syringe body fixing block 52 is fixedly connected to the body 62 of the syringe 6, and the syringe push rod fixing slider 51 is fixedly connected to the push rod 63 of the syringe 6. The syringe 6 is a commercially available medical syringe with a Luer connector. The horizontal electric slide 5 drives the push rod 63 to reciprocate to achieve liquid suction and discharge.

[0033] The three-way valve 61 is connected to the first channel 421 and the second channel 422 of the pipette head connector 42 through the first pipeline 71 and the second pipeline 72 respectively. The three-way valve 61 is a commercially available medical three-way valve with a Luer connector, and the first pipeline 71 and the second pipeline 72 are made of silicone.

[0034] The first XZ combination electric slide 2 is located above the second XZ combination electric slide 4, arranged in parallel and in the same plane, the vertical electric slide 1 is arranged below the first XZ combination electric slide 2 and perpendicular to it, and the horizontal electric slide 5 is in the same plane as the vertical electric slide 1 and perpendicular to it.

[0035] The control system 9 is connected to the vertical electric slide 1, the first XZ combination electric slide 2, the second XZ combination electric slide 4, the horizontal electric slide 5, the electrochemical double electrode 22, the diaphragm pump 34, the electric push rod 425, and the bar code recognition device 8 through cables. The control system 9 also includes a printer 91, a display 92 and a control button 93.

[0036] The housing 10 includes a sample inlet 101 and a liquid inlet 102 . The sample inlet 101 is used as a channel for placing a single-person test kit 14 (a medical device), and the liquid inlet 102 is used as an inlet and outlet channel for cleaning liquid and waste liquid.

[0037] The vertical electric slide 1, the first XZ combination electric slide 2, the second XZ combination electric slide 4 and the horizontal electric slide 5 are commercially available fully enclosed electric slides, the electric push rod 425 is a commercially available ordinary micro electric push rod, and the barcode recognition device 8 is a commercially available conventional barcode scanning and recognition device.

[0038] The single-person test kit 14 includes a sample position 141, an electrolyte position 142, a sample aspiration needle position 143, an electrolyte aspiration needle position 144 and an electrochemical sensor position 145. The sample position 141 is used to hold clinical samples, generally blood samples, etc. The electrolyte position 142 is used to hold electrolyte, generally PBS buffer, etc. The sample aspiration needle position 143 is provided with a first sample aspiration needle 1431 and a second sample aspiration needle 1432, generally 0.1~1ml pipette tips, the electrolyte aspiration needle position 144 is provided with a first electrolyte aspiration needle 1441 and a second electrolyte aspiration needle 1442, generally 1~5ml pipette tips, and the electrochemical sensor position 145 is used for sample reaction and electrolyte detection.

[0039] like Figure 6 As shown, the control detection process of the control system 9 is briefly described as follows:

[0040] S01 . A doctor places a patient's physiological sample fluid into the sample position 141 of the single-dose test kit 14 .

[0041] S02, then put the single-person reagent kit 14 into the sensor reagent kit slot 13 through the sample inlet 101, and 1 to 8 reagent kits can be put in according to the number of tests.

[0042] S03 , the control system 9 reads the reagent kit information through the barcode recognition device 8 .

[0043] S04, execute the sample aspiration program, by controlling the vertical electric slide 1 to move linearly, and drive the single-person test kit 14 to move directly below the second XZ combination electric slide 4, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the pipette head connector 42 to move above the sample aspiration needle position 143, and move downward so that the first channel 421 and the second channel 422 are respectively connected to the first sample aspiration needle 1431 and the second sample aspiration needle 1432 through interference fit to achieve fixation and channel communication, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the first sample aspiration needle 1431 and the second sample aspiration needle 1432 to the bottom of the test kit sample position 141, and by controlling the horizontal electric slide 5 to drive the syringe 6 to push The rod moves and stretches to complete the liquid aspiration, and the second XZ combined electric slide 4 is controlled to move horizontally or up and down, and the first sample aspiration needle 1431 and the second sample aspiration needle 1432 are driven to the bottom of the electrochemical sensor position 145 of the reagent kit, and the push rod of the syringe 6 is driven to move and compress to complete the liquid discharge, and the second XZ combined electric slide 4 is controlled to move horizontally or up and down, and the first sample aspiration needle 1431 and the second sample aspiration needle 1432 are driven to the top of the sample aspiration needle position 143, and the first sleeve 423 and the second sleeve 424 are driven downward by the electric push rod 425 to remove the used first sample aspiration needle 1431 and the second sample aspiration needle 1432; so far, the loading of one sample is completed, and this step is repeated to complete the loading of eight samples.

[0044] S05. Wait for the sample to react with the sensor for 10 to 30 minutes.

[0045] S06, execute the sample aspiration program, by controlling the vertical electric slide 1 to move linearly, and drive the single-person test kit 14 to move to the bottom of the second XZ combination electric slide 4, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the pipette head connector 42 to move above the sample aspiration needle position 143, and move downward so that the first channel 421 and the second channel 422 are respectively connected to the first sample aspiration needle 1431 and the second sample aspiration needle 1432 through interference fit to achieve fixation and channel communication, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the first sample aspiration needle 1431 and the second sample aspiration needle 1432 to the bottom of the electrochemical sensor position 145 of the test kit, and by controlling the horizontal electric The movable slide 5 drives the push rod of the syringe 6 to move and stretch to complete the aspiration, and by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the first sample aspiration needle 1431 and the second sample aspiration needle 1432 to the bottom of the reagent box sample position 141, and by controlling the horizontal electric slide 5 to drive the push rod of the syringe 6 to move and compress, the discharge is completed, and by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the first sample aspiration needle 1431 and the second sample aspiration needle 1432 to the top of the sample aspiration needle position 143, the electric push rod 425 drives the first sleeve 423 and the second sleeve 424 to move downward to remove the used first sample aspiration needle 1431 and the second sample aspiration needle 1432; the sample aspiration is completed.

[0046] S07, execute the electrolyte aspiration procedure, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the liquid aspiration head connector 42 to move to above the electrolyte aspiration needle position 144, and move downward so that the first channel 421 and the second channel 422 are respectively connected with the first electrolyte aspiration needle 1441 and the second electrolyte aspiration needle 1442 through interference fit to achieve fixation and channel communication, by controlling the second XZ combination electric slide 4 to move horizontally or up and down, and drive the first electrolyte aspiration needle 1441 and the second electrolyte aspiration needle 1442 to the bottom of the reagent box electrolyte level 142, and by controlling the horizontal electric slide 5 to drive the push rod of the syringe 6 to move and stretch, the aspiration is completed, by controlling The second XZ combination electric slide 4 moves horizontally or up and down, and drives the first electrolyte suction needle 1441 and the second electrolyte suction needle 1442 to the bottom of the electrochemical sensor position 145 of the reagent kit, and controls the horizontal electric slide 5 to drive the push rod of the syringe 6 to move and compress to complete the discharge, and controls the second XZ combination electric slide 4 to move horizontally or up and down, and drives the first electrolyte suction needle 1441 and the second electrolyte suction needle 1442 to the top of the electrolyte suction needle position 144, and drives the first sleeve 423 and the second sleeve 424 to move downward through the electric push rod 425 to remove the used first electrolyte suction needle 1441 and the second electrolyte suction needle 1442; completing the electrolyte suction.

[0047] S08, execute the electrode measurement program, by controlling the first XZ combined electric slide 2 to move horizontally or up and down, and drive the electrochemical double electrode 22 to below the liquid level of the electrochemical sensor position 145 of the reagent box, the control system 9 measures the electrical signal between the double electrodes to complete the electrode measurement.

[0048] S09, execute the electrode cleaning program, control the first XZ combined electric slide 2 to move horizontally or up and down, and drive the electrochemical dual electrode 22 into the cleaning tank 31 of the electrode cleaning device 3, control the diaphragm pump 34 to pump the cleaning liquid in the cleaning liquid bottle 32 through the first cleaning liquid channel 312 and the second cleaning liquid channel 313 to spray the cleaning liquid to clean the electrochemical dual electrode 22; complete the electrode cleaning.

[0049] S10. The above process completes the measurement of one sample. Repeat steps 6-9 to complete the measurement of eight samples.

[0050] Obviously, the above embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the implementation methods thereof. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all implementation methods here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the claims of the present invention.

Claims

1. An instrument for automated detection of electrochemical sensors, characterized in that: It comprises a vertical electric slide (1) provided with a sensor kit fixing slider (11), a first XZ combined electric slide (2) provided with an electrode fixing slider (21), an electrode cleaning device (3), a second XZ combined electric slide (4) provided with a liquid pipetting head fixing slider (41), a horizontal electric slide (5) provided with a syringe push rod fixing slider (51) and a syringe body fixing block (52), a syringe (6) provided with a three-way valve (61), a first pipeline (71), a second pipeline (72), a bar code recognition device (8) and a control system (9); The sensor kit fixing slider (11) is fixedly connected to a tray (12) provided with a plurality of sensor kit slots (13), wherein the sensor kit slots (13) are provided with single-portion kits (14); the electrode fixing slider (21) is fixedly connected to an electrochemical double electrode (22); the pipette head fixing slider (41) is fixedly connected to a pipette head connector (42); the syringe body fixing block (52) is fixedly connected to a body (62) of the syringe (6); the syringe push rod fixing slider (51) is fixedly connected to a push rod (63) of the syringe (6); the three-way valve (61) is connected to a first channel (421) and a second channel (422) of the pipette head connector (42) through the first pipeline (71) and the second pipeline (72), respectively; the first XZ combined electric slide (2) is arranged in parallel with the second XZ combined electric slide (4); and the vertical electric slide (1) is arranged vertically with the first XZ combined electric slide (2).

2. An instrument for automatic detection of electrochemical sensors according to claim 1, characterized in that: The electrode cleaning device (3) comprises a cleaning tank (31), a cleaning liquid bottle (32), a waste liquid bottle (33) and a diaphragm pump (34); the cleaning tank (31) is provided with an electrode channel (311), a first cleaning liquid channel (312) and a second cleaning liquid channel (313); the first cleaning liquid channel (312) and the second cleaning liquid channel (313) are in communication with the electrode channel (311); the other ends of the first cleaning liquid channel (312) and the second cleaning liquid channel (313) are in communication with the cleaning liquid bottle (32) via the diaphragm pump (34); and the lower end of the electrode channel (311) is in communication with the waste liquid bottle (33).

3. The instrument for automatic detection of electrochemical sensors according to claim 1, characterized in that: The pipette head connector (42) further comprises a first sleeve (423) arranged outside the first channel (421), a second sleeve (424) arranged outside the second channel (422), and an electric push rod (425); the first sleeve (423) is coaxial with the first channel (421) and movably connected; the second sleeve (424) is coaxial with the second channel (422) and movably connected; the movable end of the electric push rod (425) is fixedly connected to the first sleeve (423) and the second sleeve (424).

4. The instrument for automatic detection of electrochemical sensors according to claim 1, characterized in that: The syringe (6) is connected to the three-way valve (61) via a Luer connector.

5. The instrument for automatic detection of electrochemical sensors according to claim 1, characterized in that: The first pipeline (71) and the second pipeline (72) are made of silicone.

6. The instrument for automatic detection of electrochemical sensors according to claim 1, characterized in that: The control system (9) is connected to the vertical electric slide (1), the first XZ combined electric slide (2), the second XZ combined electric slide (4), the horizontal electric slide (5), the electrochemical double electrode (22), the diaphragm pump (34), the electric push rod (425) and the bar code recognition device (8) respectively through cables.

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