Root soil activation method to promote root growth

Abstract

The present invention discloses a root soil activation method that can promote root growth, and belongs to the field of agricultural technology. The root soil activation method that can promote root growth includes the following steps: step 1: first, the soil is tested and evaluated, and then organic matter is applied, the soil pH is adjusted, and pre-treated soil is obtained; step 2: after adding a biostimulant to the pre-treated soil, the soil is left to stand, and crops are transplanted into the treated soil; step 3: spraying a spray containing a root promoter onto the roots of the crop, and then irrigating the crop using micro-sprinkler irrigation technology; step 4: during the growth period, the soil is loosened and weeded regularly, and the crop is harvested after maturity. Through this implementation step, the root growth and stress resistance of the crop can be effectively improved.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and in particular relates to a root soil activation method capable of promoting root growth. Background Art

[0002] As the main component of plants, the root system is responsible for absorbing water and nutrients from the soil and fixing the plant body. The root system evolved after plants landed, and is a vital organ for obtaining most nutrients and water. The root system interacts closely with the soil environment through its complex structure and secretions, thereby effectively obtaining and activating nutrients in the soil. The diversity of root system structure, such as the distribution of fine roots in the surface soil and the construction of the root system of coniferous trees in the deep soil, are all to adapt to different soil environments and nutrient conditions.

[0003] With the development of modern agriculture, soil problems have become increasingly prominent, such as soil compaction, soil pH imbalance, and insufficient soil nutrients. These problems have seriously affected the normal growth and function of the root system. Soil compaction will reduce the soil's ability to retain water and fertilizer and its permeability, causing the root system to lose vitality due to lack of oxygen, affecting its absorption of water and nutrients. Soil pH imbalance will directly affect the root growth environment and nutrient absorption efficiency. For example, increased heavy metal activity in acidic soils will be toxic to the root system, while excessive salt ions in alkaline soils will cause root osmotic stress or even poisoning. Therefore, the root system can be activated and the healthy growth of plants can be promoted by improving the soil environment, increasing soil fertility, and optimizing root configuration and secretions.

[0004] CN117502179A discloses a special nutrient covering soil for rice seedling medium and a preparation method thereof, wherein the special nutrient covering soil is made of 80-85 parts of dry fine garden soil, 15-20 parts of powdered organic fertilizer and 1-2 parts of nutrient covering soil synergist by weight. The raw material formula is simple and reasonable, the nutrition is comprehensive and balanced, and sterilization, insect prevention, nutrition, root promotion and regulation are integrated, which can meet the nutrient needs of rice seedling growth, enhance the immunity of rice seedlings, cultivate good environmental conditions for rice seedling growth, and promote the healthy and vigorous growth of rice seedlings. However, the nutrient covering soil prepared by the method still has room for improvement in improving the root growth and stress resistance of rice seedlings. Summary of the invention

[0005] The purpose of the present invention is to provide a root soil activation method that can promote root growth, so as to solve the technical problems of poor root growth and stress resistance of crops in the prior art.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] The present invention provides a root soil activation method for promoting root growth, comprising the following steps:

[0008] Step 1: First, test and evaluate the soil, then apply organic matter and adjust the soil pH to obtain pretreated soil;

[0009] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0010] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0011] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0012] Preferably, in step 1, the organic matter applied includes one or more of compost, green manure, humic acid and crop straw, and the soil pH is adjusted to 6.0-7.5 by using lime and gypsum.

[0013] Preferably, the method for preparing the biostimulant in step 2 comprises the following steps:

[0014] Q1: Add 3-methylmercaptopropanol and triethylamine to a container containing tetrahydrofuran, add tetrahydrofuran containing acryloyl chloride dropwise through a constant pressure dropping funnel, stir at low temperature, stir at room temperature, filter, dissolve, wash, and dry to obtain a light yellow liquid; add L-cysteine ​​to a container filled with deionized water, adjust the pH, add the light yellow liquid, stir at room temperature, precipitate, wash, and dry to obtain a white powder;

[0015] Q2: Add the white powder to a container containing tetrahydrofuran, add di(trichloromethyl) carbonate after stirring at room temperature, heat and stir, remove the solvent in vacuo, dissolve, wash, dry, purify, dissolve, purify, dry to obtain a colorless liquid; add the colorless liquid to a container containing tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of n-butylamine and N,N-dimethylformamide, stir, precipitate, wash, filter, and dry in vacuo to obtain a light yellow solid;

[0016] Q3: Add a light yellow solid into a container, add tetrahydrofuran to dissolve it, then add hydrogen peroxide, heat and stir, dilute, dialyze, and vacuum remove the solvent to obtain a biostimulant.

[0017] In the above process, the synthetic reaction formula of the biostimulant is as follows:

[0018]

[0019] The results of mass spectrometry analysis of the light yellow liquid were: m / z: 160.06 (100.0%), 161.06 (8.6%), 162.05 (4.5%); the results of mass spectrometry analysis of the white powder were: m / z: 281.08 (100.0%), 282.08 (11.2%), 283.07 (9.1%), 282.07 (2.0%), 283.08 (1.6%), 284.07 (1.1%).

[0020] Preferably, in Q1, the amount ratio of 3-methylmercaptopropanol, triethylamine and acryloyl chloride is (4.11-4.33) g: (3.12-3.6) g: (2.8-3.2) g, the low-temperature stirring temperature is 0-1°C, the stirring time is 2-4 h, the room temperature stirring time is 14-16 h, it is dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 30-45 min; the amount ratio of L-cysteine, deionized water and light yellow liquid is (2.5-2.9) g: (30-45) mL: (3.2-3.9) g, the pH is adjusted to 7.2-7.5 with 1 mol / L sodium hydroxide aqueous solution, the room temperature stirring time is 15-17 h, precipitated with ethanol, and washed with ethyl acetate.

[0021] Preferably, in Q2, the amount ratio of white powder, tetrahydrofuran and di(trichloromethyl)carbonate is (1.21-1.65) g: (40-60) mL: (0.51-0.72) g, the heating and stirring temperature is 50-60°C, the stirring time is 5-7h, washed with saturated sodium bicarbonate and saturated sodium chloride, and dried with anhydrous sodium sulfate for 30-45min; the amount ratio of colorless liquid, tetrahydrofuran, n-butane and N,N-dimethylformamide is (0.4-0.52) g: (3-5) mL: (0.1-0.2) mL: (5-7) μL, the stirring time is 68-72h, added to ether for precipitation, and washed with deionized water.

[0022] Preferably, in Q3, the ratio of the pale yellow solid, tetrahydrofuran and hydrogen peroxide is (0.05-0.15) g: (2-4) mL: (0.17-0.18) mL, the volume fraction of hydrogen peroxide is 30 vt%, the heating and stirring temperature is 40-45 ° C, the stirring time is 20-24 h, deionized water is added for dilution, and dialyzed for 3-5 days using a dialysis bag with a molecular weight cutoff of 1500 Da.

[0023] Preferably, the preparation method of the root promoter comprises the following steps:

[0024] S1: Add 3-(4-hydroxyphenyl)propionic acid and N-hydroxysuccinimide to a container, then add ethyl acetate to stir and dissolve, then place in a low temperature environment, dissolve N,N'-dicyclohexylcarboximide with ethyl acetate, then slowly add dropwise to the container, stir, filter, collect the precipitate, recrystallize, and obtain a white solid;

[0025] S2: Add hydroxyethyl chitosan to 4-morpholineethanesulfonic acid, stir to obtain solution 1, then add the white solid to methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature, drop acetic anhydride, continue to react, after the reaction is completed, rotary evaporate, precipitate, centrifuge, dissolve, dialyze, and vacuum dry to obtain a root promoter.

[0026] In the above process, the synthetic reaction formula of the root promoter is as follows:

[0027]

[0028] The results of mass spectrometry analysis of the white solid were: m / z: 263.08 (100.0%), 264.08 (14.6%), 265.08(1.1%), 265.09 (1.0%).

[0029] Preferably, in S1, the usage ratio of 3-(4-hydroxyphenyl)propionic acid, N-hydroxysuccinimide and N,N'-dicyclohexylcarboximide is (1.44-1.88) g: (1-1.3) g: (2.01-2.11) mg, the low temperature environment temperature is 0-1°C, and recrystallization is carried out in isopropanol.

[0030] Preferably, in S2, the dosage ratio of hydroxyethyl chitosan, 4-morpholineethanesulfonic acid, white solid, methanol and acetic anhydride is (1-1.5) g: (50-75) mL: (0.034-0.092) g: (50-75) mL: (4.8-4.94) g, the pH of 4-morpholineethanesulfonic acid is 5-5.3, the stirring time at room temperature is 45-50 h, the reaction time is 20-24 h, it is added to acetone for precipitation, dissolved with 1 mol / L sodium hydroxide solution, the treatment time is 10-14 h, and dialyzed with a dialysis bag with a molecular weight cutoff of 3500 Da.

[0031] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0032] The invention firstly uses 3-methylmercaptopropanol, acryloyl chloride, L-cysteine, bis(trichloromethyl)carbonate, n-butylamine and hydrogen peroxide as raw materials to prepare a biostimulant, and then uses 3-(4-hydroxyphenyl)propionic acid, N-hydroxysuccinimide, hydroxyethyl chitosan and acetic anhydride as raw materials to prepare a root promoter. The biostimulant and the root promoter are used in the root soil activation method, which can effectively improve the root growth of crops, enhance the stress resistance of crops, and improve the soil environment. The active ingredients in the biostimulant and the root promoter It can stimulate the division and elongation of crop root cells, thereby promoting root growth. It can also optimize the root structure, make the root system more developed, and improve the plant's ability to absorb water and nutrients. The active ingredients in the root promoter can induce crops to produce resistance factors to adverse environmental conditions. Resistance factors can help crops resist the impact of adverse environments and improve the survival ability of crops. The presence of both can also decompose organic matter in the soil, release nutrients that can be absorbed by plants, and at the same time combine with harmful substances in the soil to reduce their toxicity and improve the soil environment. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] Example 1: This example discloses a method for preparing a biostimulant, comprising the following steps:

[0035] Q1: 4.22 g 3-methylmercaptopropanol and 3.36 g triethylamine were added to a container containing 40 mL tetrahydrofuran, and 12 mL tetrahydrofuran containing 3 g acryloyl chloride was added dropwise through a constant pressure dropping funnel. After stirring at 0°C for 4 h, the mixture was stirred at room temperature for 16 h, filtered, dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 45 min to obtain a light yellow liquid; 2.7 g L-cysteine ​​was added to a container containing 37.5 mL deionized water, and the pH was adjusted to 7.2 with 1 mol / L sodium hydroxide aqueous solution, and then 3.55 g of the light yellow liquid was added. The mixture was stirred at room temperature for 16 h, precipitated with ethanol, washed with ethyl acetate, and dried to obtain a white powder;

[0036] Q2: Add 1.43 g of white powder to a container containing 50 mL of tetrahydrofuran, stir at room temperature, add 0.65 g of di(trichloromethyl) carbonate, heat and stir at 60°C for 6 h, remove the solvent in vacuo, dissolve, wash with saturated sodium bicarbonate and saturated sodium chloride, dry with anhydrous sodium sulfate for 30 min, purify, dissolve, purify, and dry to obtain a colorless liquid; add 0.46 g of colorless liquid to a container containing 4 mL of tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of 0.15 mL of n-butylamine and 6 μL of N,N-dimethylformamide, stir for 72 h, add to ether for precipitation, wash with deionized water, filter, and dry in vacuo to obtain a light yellow solid;

[0037] Q3: Add 0.1 g of light yellow solid into a container, add 3 mL of tetrahydrofuran to dissolve it, then add 0.175 mL of 30 vt% hydrogen peroxide, heat and stir at 45 °C for 24 h, add deionized water to dilute it, dialyze it with a dialysis bag with a molecular weight cutoff of 1500 Da for 5 days, and remove the solvent in vacuo to obtain the biostimulant.

[0038] This embodiment discloses a method for preparing a root promoter, comprising the following steps:

[0039] S1: 1.66 g 3-(4-hydroxyphenyl)propionic acid and 1.15 g N-hydroxysuccinimide were added to a container, and then 30 mL ethyl acetate was added for stirring and dissolving, and then placed in a low temperature environment of 0°C, 2.06 mg N, N'-dicyclohexylcarboximide was dissolved in 15 mL ethyl acetate, and then slowly added dropwise to the container, stirred, filtered, and the precipitate was collected and recrystallized in isopropanol to obtain a white solid;

[0040] S2: Add 1.25g of hydroxyethyl chitosan to 57.5mL of 4-morpholineethanesulfonic acid (pH=5.2), stir to obtain solution 1, then add 0.063g of white solid to 62.5mL of methanol, stir to obtain solution 2, slowly add solution 2 dropwise to solution 1, stir at room temperature for 48h, then add 4.87g of acetic anhydride dropwise, continue to react for 24h, after the reaction is completed, rotary evaporate, add to acetone for precipitation, centrifuge, dissolve in 1mol / L sodium hydroxide solution for 14h, dialyze with a dialysis bag with a molecular weight cutoff of 3500Da, and vacuum dry to obtain a root promoter.

[0041] This embodiment discloses a root soil activation method that can promote root growth, comprising the following steps:

[0042] Step 1: First, the soil was tested and evaluated, and then humic acid was applied. The soil pH was adjusted to 6.6 by using lime and gypsum to obtain pre-treated soil.

[0043] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0044] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0045] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0046] Example 2: This example discloses a method for preparing a biostimulant, comprising the following steps:

[0047] Q1: 4.11 g 3-methylmercaptopropanol and 3.12 g triethylamine were added to a container containing 40 mL tetrahydrofuran, and 12 mL tetrahydrofuran containing 2.8 g acryloyl chloride was added dropwise through a constant pressure dropping funnel. After stirring at 0°C for 4 h, the mixture was stirred at room temperature for 16 h, filtered, dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 45 min to obtain a light yellow liquid; 2.5 g L-cysteine ​​was added to a container containing 30 mL deionized water, and the pH was adjusted to 7.2 with 1 mol / L sodium hydroxide aqueous solution, and then 3.9 g of the light yellow liquid was added. The mixture was stirred at room temperature for 16 h, precipitated with ethanol, washed with ethyl acetate, and dried to obtain a white powder;

[0048] Q2: Add 1.21 g of white powder to a container containing 60 mL of tetrahydrofuran, add 0.51 g of di(trichloromethyl) carbonate after stirring at room temperature, heat and stir at 60°C for 6 h, remove the solvent in vacuo, dissolve, wash with saturated sodium bicarbonate and saturated sodium chloride, dry with anhydrous sodium sulfate for 30 min, purify, dissolve, purify, and dry to obtain a colorless liquid; add 0.4 g of colorless liquid to a container containing 5 mL of tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of 0.2 mL of n-butylamine and 7 μL of N,N-dimethylformamide, stir for 72 h, add to ether for precipitation, wash with deionized water, filter, and dry in vacuo to obtain a light yellow solid;

[0049] Q3: Add 0.05 g of light yellow solid into a container, add 2 mL of tetrahydrofuran to dissolve it, then add 0.17 mL of 30 vt% hydrogen peroxide, heat and stir at 45 °C for 24 h, add deionized water to dilute it, dialyze it with a dialysis bag with a molecular weight cutoff of 1500 Da for 5 days, and remove the solvent in vacuo to obtain the biostimulant.

[0050] This embodiment discloses a method for preparing a root promoter, comprising the following steps:

[0051] S1: 1.44 g 3-(4-hydroxyphenyl)propionic acid and 1.3 g N-hydroxysuccinimide were added to a container, and then 30 mL ethyl acetate was added for stirring and dissolving, and then placed in a low temperature environment of 0°C, 2.01 mg N,N'-dicyclohexylcarboximide was dissolved in 15 mL ethyl acetate, and then slowly added dropwise to the container, stirred, filtered, and the precipitate was collected and recrystallized in isopropanol to obtain a white solid;

[0052] S2: Add 1.5 g of hydroxyethyl chitosan to 50 mL of 4-morpholineethanesulfonic acid (pH=5.2), stir to obtain solution 1, then add 0.034 g of white solid to 50 mL of methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature for 48 h, drop 4.8 g of acetic anhydride, continue to react for 24 h, after the reaction is completed, rotary evaporate, add to acetone for precipitation, centrifuge, dissolve in 1 mol / L sodium hydroxide solution for 14 h, dialyze with a dialysis bag with a molecular weight cutoff of 3500 Da, and vacuum dry to obtain a root promoter.

[0053] This embodiment discloses a root soil activation method that can promote root growth, comprising the following steps:

[0054] Step 1: First, the soil was tested and evaluated, and then humic acid was applied. The soil pH was adjusted to 6.6 by using lime and gypsum to obtain pre-treated soil.

[0055] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0056] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0057] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0058] Example 3: This example discloses a method for preparing a biostimulant, comprising the following steps:

[0059] Q1: 4.33 g 3-methylmercaptopropanol and 3.6 g triethylamine were added to a container containing 40 mL tetrahydrofuran, and 12 mL tetrahydrofuran containing 3.2 g acryloyl chloride was added dropwise through a constant pressure dropping funnel. After stirring at 0°C for 4 h, the mixture was stirred at room temperature for 16 h, filtered, dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 45 min to obtain a light yellow liquid; 2.9 g L-cysteine ​​was added to a container containing 45 mL deionized water, and the pH was adjusted to 7.2 with 1 mol / L sodium hydroxide aqueous solution, and then 3.2 g of the light yellow liquid was added, stirred at room temperature for 16 h, precipitated with ethanol, washed with ethyl acetate, and dried to obtain a white powder;

[0060] Q2: Add 1.65 g of white powder to a container containing 40 mL of tetrahydrofuran, stir at room temperature, add 0.72 g of di(trichloromethyl) carbonate, heat and stir at 60°C for 6 h, remove the solvent in vacuo, dissolve, wash with saturated sodium bicarbonate and saturated sodium chloride, dry with anhydrous sodium sulfate for 30 min, purify, dissolve, purify, and dry to obtain a colorless liquid; add 0.52 g of colorless liquid to a container containing 3 mL of tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of 0.1 mL of n-butylamine and 5 μL of N,N-dimethylformamide, stir for 72 h, add to ether for precipitation, wash with deionized water, filter, and dry in vacuo to obtain a light yellow solid;

[0061] Q3: Add 0.15 g of light yellow solid into a container, add 4 mL of tetrahydrofuran to dissolve it, then add 0.18 mL of 30 vt% hydrogen peroxide, heat and stir at 45 °C for 24 h, add deionized water to dilute it, dialyze it with a dialysis bag with a molecular weight cutoff of 1500 Da for 5 days, and remove the solvent in vacuo to obtain the biostimulant.

[0062] This embodiment discloses a method for preparing a root promoter, comprising the following steps:

[0063] S1: 1.88 g of 3-(4-hydroxyphenyl)propionic acid and 1 g of N-hydroxysuccinimide were added to a container, and then 30 mL of ethyl acetate was added for stirring and dissolving, and then placed in a low temperature environment of 0°C, 2.11 mg of N,N'-dicyclohexylcarboximide was dissolved in 15 mL of ethyl acetate, and then slowly added dropwise to the container, stirred, filtered, and the precipitate was collected and recrystallized in isopropanol to obtain a white solid;

[0064] S2: Add 1 g of hydroxyethyl chitosan to 75 mL of 4-morpholineethanesulfonic acid (pH=5.2), stir to obtain solution 1, then add 0.092 g of white solid to 75 mL of methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature for 48 h, drop 4.92 g of acetic anhydride, continue to react for 24 h, after the reaction is completed, rotary evaporate, add to acetone for precipitation, centrifuge, dissolve in 1 mol / L sodium hydroxide solution for 14 h, dialyze with a dialysis bag with a molecular weight cutoff of 3500 Da, and vacuum dry to obtain a root promoter.

[0065] This embodiment discloses a root soil activation method that can promote root growth, comprising the following steps:

[0066] Step 1: First, the soil was tested and evaluated, and then humic acid was applied. The soil pH was adjusted to 6.6 by using lime and gypsum to obtain pre-treated soil.

[0067] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0068] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0069] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0070] Example 4: This example discloses a method for preparing a biostimulant, comprising the following steps:

[0071] Q1: 4.15 g 3-methylmercaptopropanol and 3.24 g triethylamine were added to a container containing 40 mL tetrahydrofuran, and 12 mL tetrahydrofuran containing 2.9 g acryloyl chloride was added dropwise through a constant pressure dropping funnel. After stirring at 0°C for 4 h, the mixture was stirred at room temperature for 16 h, filtered, dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 45 min to obtain a light yellow liquid; 2.6 g L-cysteine ​​was added to a container containing 35 mL deionized water, and the pH was adjusted to 7.2 with 1 mol / L sodium hydroxide aqueous solution, and then 3.4 g of the light yellow liquid was added. The mixture was stirred at room temperature for 16 h, precipitated with ethanol, washed with ethyl acetate, and dried to obtain a white powder;

[0072] Q2: Add 1.37 g of white powder to a container containing 45 mL of tetrahydrofuran, stir at room temperature, add 0.54 g of di(trichloromethyl) carbonate, heat and stir at 60°C for 6 h, remove the solvent in vacuo, dissolve, wash with saturated sodium bicarbonate and saturated sodium chloride, dry with anhydrous sodium sulfate for 30 min, purify, dissolve, purify, and dry to obtain a colorless liquid; add 0.43 g of colorless liquid to a container containing 3.5 mL of tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of 0.12 mL of n-butylamine and 5.5 μL of N,N-dimethylformamide, stir for 72 h, add to ether for precipitation, wash with deionized water, filter, and dry in vacuo to obtain a light yellow solid;

[0073] Q3: Add 0.08 g of light yellow solid into a container, add 2.5 mL of tetrahydrofuran to dissolve it, then add 0.18 mL of 30 vt% hydrogen peroxide, heat and stir at 45 °C for 24 h, add deionized water to dilute it, dialyze it with a dialysis bag with a molecular weight cutoff of 1500 Da for 5 days, and remove the solvent in vacuo to obtain the biostimulant.

[0074] This embodiment discloses a method for preparing a root promoter, comprising the following steps:

[0075] S1: 1.55 g of 3-(4-hydroxyphenyl)propionic acid and 1.2 g of N-hydroxysuccinimide were added to a container, and then 30 mL of ethyl acetate was added for stirring and dissolving, and then placed in a low temperature environment of 0°C, 2.03 mg of N,N'-dicyclohexylcarboximide was dissolved in 15 mL of ethyl acetate, and then slowly added dropwise to the container, stirred, filtered, and the precipitate was collected and recrystallized in isopropanol to obtain a white solid;

[0076] S2: Add 1.1 g of hydroxyethyl chitosan to 55 mL of 4-morpholineethanesulfonic acid (pH=5.2), stir to obtain solution 1, then add 0.041 g of white solid to 55 mL of methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature for 48 h, drop 4.83 g of acetic anhydride, continue to react for 24 h, after the reaction is completed, rotary evaporate, add to acetone for precipitation, centrifuge, dissolve in 1 mol / L sodium hydroxide solution for 14 h, dialyze with a dialysis bag with a molecular weight cutoff of 3500 Da, and vacuum dry to obtain a root promoter.

[0077] This embodiment discloses a root soil activation method that can promote root growth, comprising the following steps:

[0078] Step 1: First, the soil was tested and evaluated, and then humic acid was applied. The soil pH was adjusted to 6.6 by using lime and gypsum to obtain pre-treated soil.

[0079] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0080] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0081] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0082] Example 5: This example discloses a method for preparing a biostimulant, comprising the following steps:

[0083] Q1: 4.25 g 3-methylmercaptopropanol and 3.48 g triethylamine were added to a container containing 40 mL tetrahydrofuran, and 12 mL tetrahydrofuran containing 3.1 g acryloyl chloride was added dropwise through a constant pressure dropping funnel. After stirring at 0°C for 4 h, the mixture was stirred at room temperature for 16 h, filtered, dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 45 min to obtain a light yellow liquid; 2.8 g L-cysteine ​​was added to a container containing 40 mL deionized water, and the pH was adjusted to 7.2 with 1 mol / L sodium hydroxide aqueous solution, and then 3.8 g of the light yellow liquid was added, stirred at room temperature for 16 h, precipitated with ethanol, washed with ethyl acetate, and dried to obtain a white powder;

[0084] Q2: Add 1.58 g of white powder to a container containing 55 mL of tetrahydrofuran, stir at room temperature, add 0.68 g of di(trichloromethyl) carbonate, heat and stir at 60°C for 6 h, remove the solvent in vacuo, dissolve, wash with saturated sodium bicarbonate and saturated sodium chloride, dry with anhydrous sodium sulfate for 30 min, purify, dissolve, purify, and dry to obtain a colorless liquid; add 0.48 g of colorless liquid to a container containing 4.5 mL of tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of 0.18 mL of n-butylamine and 6.5 μL of N,N-dimethylformamide, stir for 72 h, add to ether for precipitation, wash with deionized water, filter, and dry in vacuo to obtain a light yellow solid;

[0085] Q3: Add 0.12 g of light yellow solid into a container, add 3.5 mL of tetrahydrofuran to dissolve it, then add 0.17 mL of 30 vt% hydrogen peroxide, heat and stir at 45 °C for 24 h, add deionized water to dilute it, dialyze it with a dialysis bag with a molecular weight cutoff of 1500 Da for 5 days, and remove the solvent in vacuo to obtain the biostimulant.

[0086] This embodiment discloses a method for preparing a root promoter, comprising the following steps:

[0087] S1: 1.77 g 3-(4-hydroxyphenyl)propionic acid and 1.1 g N-hydroxysuccinimide were added to a container, and then 30 mL ethyl acetate was added for stirring and dissolving, and then placed in a low temperature environment of 0°C, 2.09 mg N,N'-dicyclohexylcarboximide was dissolved in 15 mL ethyl acetate, and then slowly added dropwise to the container, stirred, filtered, and the precipitate was collected and recrystallized in isopropanol to obtain a white solid;

[0088] S2: Add 1.4 g of hydroxyethyl chitosan to 70 mL of 4-morpholineethanesulfonic acid (pH=5.2), stir to obtain solution 1, then add 0.087 g of white solid to 70 mL of methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature for 48 h, drop 4.92 g of acetic anhydride, continue to react for 24 h, after the reaction is completed, rotary evaporate, add to acetone for precipitation, centrifuge, dissolve in 1 mol / L sodium hydroxide solution for 14 h, dialyze with a dialysis bag with a molecular weight cutoff of 3500 Da, and vacuum dry to obtain a root promoter.

[0089] This embodiment discloses a root soil activation method that can promote root growth, comprising the following steps:

[0090] Step 1: First, the soil was tested and evaluated, and then humic acid was applied. The soil pH was adjusted to 6.6 by using lime and gypsum to obtain pre-treated soil.

[0091] Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil;

[0092] Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology;

[0093] Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature.

[0094] Comparative Example 1: Compared with Example 1, in Comparative Example 1, during the implementation of the root soil activation method for promoting root growth, no biostimulant was added, and other conditions remained unchanged.

[0095] Comparative Example 2: Compared with Example 1, in Comparative Example 2, during the implementation of the root soil activation method that can promote root growth, no root promoter is added, and other conditions remain unchanged.

[0096] Experimental Example: Corn was planted according to the methods of the embodiment and the comparative example, and the root quantity, root length and plant height of the corn were observed and measured. The test results are shown in Table 1:

[0097]

[0098] From the test results in Table 1, it can be seen that planting corn according to Examples 1-5 can effectively increase the number of roots and plant height. From the comparison between Comparative Example 1 and Examples 1-5, it can be seen that the use of biostimulants can effectively increase the number of roots and plant height of corn; from the comparison between Comparative Example 2 and Examples 1-5, it can be seen that the use of root promoters can effectively increase the number of roots and plant height of corn.

[0099] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

[0100] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to only specific implementation methods. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A root soil activation method for promoting root growth, characterized in that: The following steps are involved: Step 1: First, test and evaluate the soil, then apply organic matter and adjust the soil pH to obtain pretreated soil; Step 2: After adding biostimulants to the pre-treated soil, let it stand and transplant crops into the treated soil; Step 3: Spray the root promoter onto the roots of the crops, and then irrigate the crops using micro-sprinkler irrigation technology; Step 4: During the growing season, loosen the soil and weed regularly, and harvest the crops when they are mature; The biostimulant in step 2 is prepared from 3-methylmercaptopropanol, acryloyl chloride, L-cysteine, di(trichloromethyl)carbonate, n-butylamine and hydrogen peroxide, and the dosage ratio is (4.11-4.33) g: (2.8-3.2) g: (2.5-2.9) g: (0.51-0.72) g: (0.1-0.2) mL: (0.17-0.18) mL; the root promoter in step 3 is prepared from 3-(4-hydroxyphenyl)propionic acid, N,N'-dicyclohexylcarboximide, hydroxyethyl chitosan and acetic anhydride, and the dosage ratio is (1.44-1.88) g: (2.01-2.11) mg: (1-1.5) g: (4.8-4.94) g; The preparation method of the biostimulant comprises the following steps: Q1: Add 3-methylmercaptopropanol and triethylamine to a container containing tetrahydrofuran, add tetrahydrofuran containing acryloyl chloride dropwise through a constant pressure dropping funnel, stir at low temperature, stir at room temperature, filter, dissolve, wash, and dry to obtain a light yellow liquid; add L-cysteine ​​to a container filled with deionized water, adjust the pH, add the light yellow liquid, stir at room temperature, precipitate, wash, and dry to obtain a white powder; Q2: Add the white powder to a container containing tetrahydrofuran, add di(trichloromethyl) carbonate after stirring at room temperature, heat and stir, remove the solvent in vacuo, dissolve, wash, dry, purify, dissolve, purify, dry to obtain a colorless liquid; add the colorless liquid to a container containing tetrahydrofuran, dissolve, pass nitrogen, add a mixed solution of n-butylamine and N,N-dimethylformamide, stir, precipitate, wash, filter, and dry in vacuo to obtain a light yellow solid; Q3: Add a light yellow solid into a container, add tetrahydrofuran to dissolve it, then add hydrogen peroxide, heat and stir, dilute, dialyze, and vacuum remove the solvent to obtain a biostimulant; The preparation method of the root promoter comprises the following steps: S1: Add 3-(4-hydroxyphenyl)propionic acid and N-hydroxysuccinimide to a container, then add ethyl acetate to stir and dissolve, then place in a low temperature environment, dissolve N,N'-dicyclohexylcarboximide with ethyl acetate, then slowly add dropwise to the container, stir, filter, collect the precipitate, recrystallize, and obtain a white solid; S2: Add hydroxyethyl chitosan to 4-morpholineethanesulfonic acid, stir to obtain solution 1, then add the white solid to methanol, stir to obtain solution 2, slowly drop solution 2 into solution 1, stir at room temperature, drop acetic anhydride, continue to react, after the reaction is completed, rotary evaporate, precipitate, centrifuge, dissolve, dialyze, and vacuum dry to obtain a root promoter.

2. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the step 1, the applied organic matter includes one or more of compost, green manure, humic acid and crop straw, and the soil pH is adjusted to 6.0-7.5 by using lime and gypsum.

3. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the Q1, the amount ratio of 3-methylmercaptopropanol, triethylamine and acryloyl chloride is (4.11-4.33) g: (3.12-3.6) g: (2.8-3.2) g, the low-temperature stirring temperature is 0-1°C, the stirring time is 2-4 h, and the room temperature stirring time is 14-16 h. It is dissolved with dichloromethane, washed with 1 mol / L hydrochloric acid, saturated sodium bicarbonate aqueous solution and saturated sodium chloride aqueous solution in sequence, and dried with anhydrous sodium sulfate for 30-45 min; the amount ratio of L-cysteine, deionized water and light yellow liquid is (2.5-2.9) g: (30-45) mL: (3.2-3.9) g, the pH is adjusted to 7.2-7.5 with 1 mol / L sodium hydroxide aqueous solution, the room temperature stirring time is 15-17 h, precipitated with ethanol, and washed with ethyl acetate.

4. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the Q2, the amount ratio of white powder, tetrahydrofuran and di(trichloromethyl) carbonate is (1.21-1.65) g: (40-60) mL: (0.51-0.72) g, the heating and stirring temperature is 50-60°C, the stirring time is 5-7 hours, washed with saturated sodium bicarbonate and saturated sodium chloride, and dried with anhydrous sodium sulfate for 30-45 minutes; the amount ratio of colorless liquid, tetrahydrofuran, n-butane and N,N-dimethylformamide is (0.4-0.52) g: (3-5) mL: (0.1-0.2) mL: (5-7) μL, the stirring time is 68-72 hours, added to ether for precipitation, and washed with deionized water.

5. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the Q3, the dosage ratio of the light yellow solid, tetrahydrofuran and hydrogen peroxide is (0.05-0.15) g: (2-4) mL: (0.17-0.18) mL, the volume fraction of hydrogen peroxide is 30 vt%, the heating and stirring temperature is 40-45°C, the stirring time is 20-24 h, deionized water is added for dilution, and dialyzed for 3-5 days using a dialysis bag with a molecular weight cutoff of 1500 Da.

6. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the S1, the usage ratio of 3-(4-hydroxyphenyl)propionic acid, N-hydroxysuccinimide and N,N'-dicyclohexylcarboximide is (1.44-1.88) g: (1-1.3) g: (2.01-2.11) mg, the low temperature environment temperature is 0-1°C, and recrystallization is carried out in isopropanol.

7. The root soil activation method for promoting root growth according to claim 1, characterized in that: In the S2, the dosage ratio of hydroxyethyl chitosan, 4-morpholineethanesulfonic acid, white solid, methanol and acetic anhydride is (1-1.5) g: (50-75) mL: (0.034-0.092) g: (50-75) mL: (4.8-4.94) g, the pH of 4-morpholineethanesulfonic acid is 5-5.3, the stirring time at room temperature is 45-50 h, the reaction time is 20-24 h, it is added to acetone for precipitation, dissolved with 1 mol / L sodium hydroxide solution, the treatment time is 10-14 h, and dialyzed with a dialysis bag with a molecular weight cutoff of 3500 Da.

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