Streptomyces avermitilis-purpureococcus texanus complex microbial agent and preparation device and method

Abstract

The application discloses a streptomyces avermitilis-purpureococcus texanus compound microbial agent and a preparation device and method thereof, which comprises a strain preparation module, a culture medium configuration module, a compound fermentation module and a centrifugal purification module, wherein the compound fermentation module comprises a fermentation tank, a detection mechanism for detecting the compound microbial agent, a control mechanism for controlling the detection time interval and a cleaning mechanism; the detection mechanism comprises a detection rack arranged in the fermentation tank in a lifting mode, a plurality of detection barrels fixed on the detection rack, a protective barrel rotatably arranged outside the detection barrel, a pH sensor arranged in the detection barrel and a driving assembly for controlling the rotation of the protective barrel, the detection barrel is provided with a detection hole, the protective barrel is provided with a protective hole, and the inner wall of the protective barrel blocks the detection hole when the protective barrel is in an initial position; and the cleaning mechanism is used for cleaning the pH sensor. The compound microorganism in the application combines the respective advantages of the three kinds of microorganisms and can more widely prevent and control various pests, nematodes and pathogenic bacteria.

Description

Technical Field

[0001] This application relates to the field of compound microbial agents, and in particular to an abamectin-streptomyces-purpureus compound agent and its preparation apparatus and method. Background Technology

[0002] Currently, root-knot nematodes directly damage the roots of vegetables, causing tumor-like protrusions, poor growth, and root rot in affected crops. Due to the impact on the root system, most affected plants initially experience slow growth in the above-ground parts, with smaller, yellowing leaves that appear as patches of nutrient deficiency. They may also fail to produce fruit or produce poor-quality fruit. In severe cases, growth may stop, internodes may shorten, and the plants may become stunted or even wilt.

[0003] While many single-species microorganisms exist, none are sufficient to achieve pest control and promote plant growth. A complex microbial agent capable of achieving both pest control and growth promotion is needed. Therefore, researchers have studied a compound agent containing avermectin-treated Streptomyces, Paecilomyces lilacinus, and Bacillus laterosporus. These three microorganisms have unique roles in agriculture and biological control. When these three microorganisms are combined in a compound form, it may produce a broader range of biological control effects and more significant advantages in agricultural applications.

[0004] The above-mentioned method for preparing compound microbial agents includes strain preparation, culture medium preparation, compound fermentation, and subsequent treatment. The following is a general preparation method: I. Strain Preparation: * *Streptomyces ivermectin*: Obtain high-yield *Streptomyces* strains from preservation institutions or laboratories. Ensure the purity and activity of the strains, and perform rejuvenation and screening if necessary. * *Paecilomyces lilacinus*: Obtain pure *Paecilomyces lilacinus* strains from preservation institutions or laboratories. Examine the biological characteristics and functions of the strains to ensure they have the expected control effect. * *Bacillus laterosporus*: Obtain strains of *Bacillus laterosporus*, ensuring they have good growth and reproductive capabilities. II. Culture Medium Preparation: * **Basic Culture Medium:** Prepare a basic culture medium suitable for the co-growth of the three microorganisms. This medium should contain appropriate amounts of carbon source, nitrogen source, inorganic salts, and necessary trace elements. Adjustments can be made according to the nutritional requirements of each strain to achieve the best compound fermentation effect. * **Optimization of Culture Medium:** Optimize the formula and conditions of the culture medium through experiments to improve the yield and activity of the compound microorganisms. Considering the avermectin synthesis requirements of *Streptomyces avermectinus*, specific precursors or inducers can be added to the culture medium. III. Co-fermentation: Inoculation: Inoculate the prepared *Streptomyces avermectinus*, *Paecilomyces lilacinus*, and *Bacillus laterosporus* strains into the basal culture medium in a certain proportion. The inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure coordinated growth during co-fermentation. Fermentation Condition Control: Control parameters such as temperature, pH, and dissolved oxygen during fermentation to create an environment suitable for the co-growth of the three microorganisms. Regularly sample and test to observe the growth of the microorganisms and the accumulation of metabolites. Fermentation Cycle: Determine the optimal fermentation cycle based on experimental results. Generally, the fermentation cycle for co-fermentation is longer than that for single-strain fermentation because the interactions between different strains and the accumulation of metabolites need to be considered. IV. Subsequent Processing: Separation and Purification: After fermentation, separate the bacterial cells and fermentation broth using methods such as centrifugation and filtration. Quality Testing: Perform quality testing on the extracted and purified products to ensure they meet relevant standards and requirements.

[0005] Regarding the aforementioned technologies, the inventors believe that the following defects exist: the bacterial agent needs to maintain a slightly acidic pH value during the fermentation process, but in the preparation of the bacterial agent, the pH value is not monitored, making it impossible to adjust the pH value in a timely manner, which affects the quality of the finished product. Summary of the Invention

[0006] To address the issue of pH monitoring failure during fermentation, this application provides an abamectin-streptomyces-purpureus compound inoculant and its preparation apparatus and method.

[0007] The apparatus for preparing an abamectin-streptomyces-purpureus compound inoculant provided in this application adopts the following technical solution:

[0008] An apparatus for preparing an abamectin-streptomyces-purpureus compound microbial agent includes a strain preparation module, a culture medium preparation module, a compound fermentation module, and a centrifugation purification module. The compound fermentation module includes a fermenter, a detection mechanism for detecting the compound microbial agent, a control mechanism for controlling the measurement time interval, and a cleaning mechanism.

[0009] The detection mechanism includes a detection frame that is lifted and lowered inside the fermenter, multiple detection cylinders fixed on the detection frame, a protective cylinder that is rotatably disposed outside the detection cylinders, a pH sensor disposed inside the detection cylinders, and a drive assembly for controlling the rotation of the protective cylinders. The detection cylinders are provided with detection holes, and the protective cylinders are provided with protective holes. When the protective cylinders are in their initial position, the inner wall of the protective cylinders seals the detection holes. The cleaning mechanism is used to clean the pH sensor.

[0010] Optionally, the cleaning mechanism includes a cleaning rack that is lifted and disposed inside the protective cylinder, a cleaning component for cleaning the pH sensor, an acceleration component for accelerating the cleaning of the pH sensor, and a circulation component for realizing the circulation of water inside the detection cylinder.

[0011] Optionally, the cleaning assembly includes two wiping elements elastically disposed on the cleaning rack and a drive unit for realizing the elastic movement of the two wiping elements, with the two wiping elements located on both sides of the pH sensor.

[0012] Optionally, the drive unit includes two slide plates, two first springs, two first electromagnets, and two drive components. The wiping component is fixed on the slide plates. The two first springs are used to elastically position the two slide plates on the cleaning rack. The drive components are used to control the reciprocating motion of the first electromagnets, and the first electromagnets are used to attract the slide plates.

[0013] Optionally, the acceleration assembly includes a storage tank, an acceleration tube, a solenoid valve, a phase change ball, and a power unit for driving the phase change ball. The storage tank is installed on the inner wall of the fermenter. One end of the acceleration tube is connected to the storage tank, and the other end is connected to the detection cylinder. The storage tank is filled with a cleaning agent for cleaning the pH sensor. The storage tank is provided with a phase change hole for placing the phase change ball, and the phase change ball contains a phase change material.

[0014] Optionally, the power unit includes a first pipe, a second pipe, a third pipe, an elastic rod, and a push rod. The bottom end of the first pipe is closed. The elastic rod is elastically disposed at the bottom end of the first pipe. The second pipe is arc-shaped, with one end connected to one end of the first pipe and the other end facing the opening of the phase change hole. One end of the third pipe faces the phase change hole and the other end is connected to the bottom end of the first pipe. The push rod is slidably disposed on the storage tank.

[0015] Optionally, the control mechanism includes a control frame rotatably disposed outside the fermentation tube, a top box, a bottom box, a liquid level sensor, a regulating pipe, and a regulating component for controlling the flow rate in the regulating pipe. The regulating pipe is configured as a flexible hose and is in a taut state. The liquid level sensor is installed inside the bottom box. The top box and the bottom box are fixedly connected to the control frame. The regulating pipe is used to connect the top box and the bottom box.

[0016] Optionally, the adjustment assembly includes an adjustment block, an adjustment rod, an adjustment wheel, and an adjustment component for controlling the movement of the adjustment rod, all fixed on the control frame. The adjustment block has a through hole for the adjustment tube to pass through, and the adjustment block also has an adjustment groove. The adjustment groove is inclined, and both sides of the adjustment groove have inclined grooves. The two ends of the adjustment rod slide within the two inclined grooves respectively. The adjustment wheel is rotatably disposed outside the adjustment rod and within the adjustment groove. When the adjustment rod is located at both ends of the inclined groove, the flow rate of the adjustment tube is at its maximum or minimum.

[0017] The preparation method of the avermectin-streptomyces-purpureus compound inoculant provided in this application adopts the following technical solution:

[0018] A method for preparing an abamectin-streptomyces-purpureus compound inoculant includes the following steps:

[0019] S1. Preparation of strains: Take out Streptomyces avermectin, Purple Purple Sporozoa, and Bacillus retroflexus to ensure that the strains have good activity;

[0020] S2. Culture medium preparation: Optimize the formula and conditions of the culture medium through experiments to improve the yield and activity of the compound bacteria;

[0021] S3: Compound fermentation: Inoculation: Inoculate the prepared Streptomyces avermectin, Paecilomyces lilacinus and Bacillus retroflexus strains into the basic culture medium in a certain proportion;

[0022] S4: Fermentation Condition Control: Control parameters such as temperature, pH, and dissolved oxygen during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; among which, a detection agency is used to monitor and control the pH value in real time.

[0023] S5: Separation and purification: After fermentation, the bacterial cells and fermentation broth are separated by centrifugation, filtration and other methods to obtain a compound bacterial agent.

[0024] The technical solution for the avermectin-streptomyces-purpureus compound inoculant provided in this application is as follows:

[0025] An avermectin-streptomyces-purpureus compound microbial agent, comprising avermectin-streptomyces, purpureus, and Bacillus retroflexus.

[0026] In summary, this application includes at least one of the following beneficial technical effects:

[0027] 1. The compound microbial formulation combines the advantages of three microorganisms, enabling broader control of various pests, nematodes, and pathogens. Synergistic effects may exist between different microorganisms, collectively enhancing control efficacy. For example, *Paecilomyces lilacinus* and *Bacillus laterosporus* may inhibit the growth of nematodes and pathogens through different mechanisms. The use of compound microbial formulations can reduce the resistance problems caused by continuous use of single microbial agents. Finally, both *Paecilomyces lilacinus* and *Bacillus laterosporus* can produce physiologically active substances that promote plant growth; the use of compound microbial formulations may more significantly promote plant growth and development. The compound microbial formulation of *Streptomyces lilacinus*, *Paecilomyces lilacinus*, and *Bacillus laterosporus* has broad application prospects and significant advantages in agriculture.

[0028] 2. After the drive motor controls the drive gear to rotate, the drive gear controls the ring gear to rotate, and the ring gear drives the protective cylinder to rotate, thus achieving the rotation effect of the protective cylinder. The compound bacterial agent is then applied, and the compound bacterial agent is detected by a pH sensor until the pH value meets the requirements. After the detection is completed, the pH sensor needs to be cleaned. Water is injected into the detection cylinder, but the water level does not need to completely submerge the pH sensor. The wiping element can absorb water. Through the reciprocating motion of the wiping element, the wiping element impacts the pH sensor. In this embodiment, the collision distance between the wiping element and the pH sensor is short, and due to water resistance, the impact force of the wiping element on the pH sensor is small and will not damage the pH sensor. The wiping element causes the water to fluctuate. With the impact wiping of the wiping element and the pH sensor and the impact of the water fluctuation, the solution adhering to the pH sensor is greatly accelerated to fall off.

[0029] 3. In the early stage of pH adjustment in the fermenter, it is necessary to shorten the measurement time interval, which requires accelerating the cleaning effect of the pH sensor. At this time, the adjusting rod needs to be pulled to the top of the adjustment tank, and the adjusting tube is fully open. In this embodiment, this time can be set as the time for rinsing the pH sensor with clean water. When the liquid level sensor detects that the water level in the bottom tank has risen to the top, the phase change ball is immediately controlled to move into the phase change hole. The cleaning agent in the storage tank is heated. Through the repeated stirring of the two wiping parts, the mixing effect of the cleaning agent is greatly accelerated, which greatly accelerates the cleaning efficiency of the pH sensor. Finally, the positions of the top tank and the bottom tank need to be switched, and the flow rate of the adjusting tube is fully opened to quickly flow the water in the bottom tank into the top tank, which is timed for the next cleaning work. In the middle and late stages of pH adjustment in the fermenter, it is only necessary to move the adjusting rod and the adjusting wheel to the middle position of the adjustment tank. Attached Figure Description

[0030] Figure 1 This is a process flow diagram of Embodiment 2 of this application;

[0031] Figure 2 This is a schematic diagram of the overall structure of Embodiment 1 of this application;

[0032] Figure 3 This is a schematic diagram of the detection mechanism, control mechanism, and cleaning mechanism in Embodiment 1 of this application;

[0033] Figure 4 This is a schematic diagram of the cleaning component in Embodiment 1 of this application;

[0034] Figure 5 This is a schematic diagram of the push rod and the storage tank in Embodiment 1 of this application;

[0035] Figure 6 yes Figure 2 Enlarged view of point A in the middle;

[0036] Figure 7 This is a schematic diagram of the adjustment component in Embodiment 1 of this application.

[0037] Reference numerals: 1. Fermentation tank; 2. Detection rack; 3. Detection cylinder; 4. Protective cylinder; 5. pH sensor; 6. Detection port; 7. Protective port; 8. Cleaning rack; 9. Wiping component; 10. Slide plate; 11. First spring; 12. First electromagnet; 13. Circulation tank; 14. Circulation pump; 15. Water pipe; 16. Drive motor; 17. Drive gear; 18. Ring gear; 19. Slide rod; 20. Storage tank; 21. Acceleration tube; 22. Solenoid valve; 23. Phase change ball; 24. 25. Phase change hole; 26. First pipe; 27. Second pipe; 28. Third pipe; 29. ​​Elastic rod; 30. Push rod; 31. Power electric push rod; 32. Handle; 33. Power electromagnet; 34. Power spring; 35. Drive component; 36. Control frame; 37. Top box; 38. Bottom box; 39. Liquid level sensor; 40. Adjusting pipe; 41. Adjusting block; 42. Adjusting rod; 43. Adjusting wheel; 44. Perforation; 45. Adjusting groove; 46. Inclined groove. Detailed Implementation

[0038] The following is in conjunction with the appendix Figure 1-7 This application will be described in further detail.

[0039] Example 1

[0040] This application discloses an apparatus for preparing an avermectin-streptomyces-purpureus compound inoculant. (Refer to...) Figures 2-5 The preparation device for the avermectin-streptomyces-purpureus compound inoculant includes a strain preparation module, a culture medium preparation module, a compound fermentation module, and a centrifugation and purification module. Strain preparation requires the selection of avermectin-streptomyces, purpureus, and Bacillus laterosporus, ensuring each strain is in good biological condition to guarantee its growth and reproduction. The culture medium is used to cultivate the compound inoculant and needs to provide adequate carbon, nitrogen, inorganic salts, and necessary trace elements. Adjustments can be made according to the nutritional requirements of each strain to achieve optimal compound fermentation. Effects: During the compound fermentation process, prepared strains of *Streptomyces avermectin*, *Paecilomyces lilacinus*, and *Bacillus laterosporus* are inoculated into the basal culture medium in a specific ratio. The inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure coordinated growth during the compound fermentation process. Temperature, pH, dissolved oxygen, and other parameters during fermentation are controlled to create a suitable environment for the co-growth of the three microorganisms. Regular sampling and testing are conducted to observe the growth of the microorganisms and the accumulation of metabolites. After fermentation, the bacterial cells and fermentation broth are separated by centrifugation and filtration. The extracted and purified products undergo quality testing to ensure they meet relevant standards and requirements. This compound microbial preparation is promoted and applied in agriculture, horticulture, and other fields to leverage its broad-spectrum, highly efficient, safe, and long-lasting biological control effects.

[0041] Reference Figures 2-5 The compound fermentation module includes a fermenter 1, a detection mechanism for detecting the compound microbial agent, a control mechanism for controlling the measurement time interval, and a cleaning mechanism. Especially in the early fermentation process, when the pH value changes significantly, the measurement time interval is shortened. However, in the middle and late stages of fermentation, the measurement time interval can be appropriately lengthened. Therefore, the control mechanism is needed to control the measurement interval. The detection mechanism detects the pH value of the compound microbial agent. After each test, the cleaning mechanism needs to clean the detection mechanism to avoid inaccurate test results due to incomplete cleaning.

[0042] The detection mechanism includes a detection frame 2 that is lifted and installed inside the fermenter 1, multiple detection cylinders 3 fixed on the detection frame 2, a protective cylinder 4 that is rotatably installed outside the detection cylinders 3, a pH sensor 5 installed inside the detection cylinders 3, and a drive assembly for controlling the rotation of the protective cylinder 4. The detection cylinders 3 are provided with detection holes 6, and the protective cylinders 4 are provided with protective holes 7. The lifting and lowering of the detection frame 2 is achieved by an electric actuator. In this embodiment, multiple detection cylinders 3 are needed to detect the compound microbial agent at different depths. However, since the structure of each detection cylinder 3 differs only in its position, this embodiment and related figures illustrate and describe using only one detection cylinder 3. A rotating ring is fixedly connected to the inner wall of the protective cylinder 4, and a rotating groove is provided on the outer wall of the detection cylinder 3. The rotating ring rotates within the rotating groove, thereby achieving the rotation effect of the protective cylinder 4 and the detection cylinder 3. The protective cylinder 4 is in its initial position. The inner wall of the protective cylinder 4 seals the detection hole 6. Only when the protective hole 7 rotates to the position of the detection hole 6 can the solution enter the detection hole 6 from the protective hole 7. The driving assembly includes a drive motor 16, a drive gear 17, and a ring gear 18. The drive motor 16 is fixed on the detection frame 2, and the detection frame 2 is also equipped with a protective box for protecting the drive motor 16. The drive gear 17 is fixedly connected to the output end of the drive motor 16, and the ring gear 18 is fixedly connected to the outer peripheral wall of the protective cylinder 4. The drive gear 17 and the ring gear 18 are meshed. After the drive motor 16 controls the drive gear 17 to rotate, the drive gear 17 controls the ring gear 18 to rotate, and the ring gear 18 drives the protective cylinder 4 to rotate, thus achieving the rotation effect of the protective cylinder 4. The compound bacterial agent is then applied, and the compound bacterial agent is detected by the pH sensor 5 until the pH value meets the requirements.

[0043] Reference Figures 2-5The cleaning mechanism is used to clean the pH sensor 5. The cleaning mechanism includes a cleaning frame 8 that is lifted and installed inside the protective cylinder 4, a cleaning component for cleaning the pH sensor 5, an acceleration component for accelerating the cleaning of the pH sensor 5, and a circulation component for circulating the water inside the detection cylinder 3. The cleaning frame 8 is U-shaped, and the horizontal part of the cleaning frame 8 is provided with a groove for the pH sensor 5 to pass through. The lifting and lowering of the cleaning frame 8 is achieved by an electric push rod or a cylinder. The cleaning component cleans the pH sensor 5, the acceleration component is used to accelerate the cleaning speed of the pH sensor 5, and the circulation component is used to circulate the cleaning water inside the protective cylinder 4. The circulation component includes a circulating water tank, a circulating pump 14, and two water pipes 15. One end of one water pipe 15 is connected to the circulating pump 14, and the other end is connected to the top of the detection cylinder 3. One end of the other water pipe 15 is connected to the bottom of the detection cylinder 3, and the other end is connected to the circulating water tank. The circulating pump 14 is installed inside the circulating water tank, which is placed in the external environment and positioned relative to the position of the detection cylinder 3 to facilitate the water circulation effect.

[0044] Reference Figures 2-5 The cleaning assembly includes two wiping elements 9 elastically mounted on a cleaning rack 8 and a drive unit for realizing the elastic movement of the two wiping elements 9. The two wiping elements 9 are located on both sides of the pH sensor 5. In this embodiment, the wiping elements 9 are sponge blocks used to clean the pH sensor 5. The drive unit includes two first sliding plates 10, two first springs 11, two first electromagnets 12, and two drive units 34. The sliding plates 10 are elastically mounted on the cleaning rack 8, and the two sliding plates 10 are located on both sides of the pH sensor 5. The wiping elements 9 are fixed to the side of the sliding plates 10 facing the pH sensor 5. The two first springs 11 are used to realize the elastic mounting of the two sliding plates 10 on the cleaning rack 8. One end of the first spring 11 is fixedly connected to the back of the sliding plate 10, and the other end is fixedly connected to the cleaning rack 8. Of course, the sliding plates 10 can also be fixedly connected to sliding rods 19. The cleaning rack 8 is equipped with... A sliding hole is provided for the sliding rod 19 to pass through. The cooperation between the sliding rod 19 and the sliding hole is used to limit the movement direction of the sliding plate 10. The driving member 34 is used to control the reciprocating motion of the first electromagnet 12. The first electromagnet 12 is used to attract the sliding plate 10. The driving member 34 can be an electric push rod or a cylinder. When water is injected into the detection cylinder 3, and the water surface does not need to completely submerge the pH sensor 5, the wiping member 9 can absorb water. Through the reciprocating motion of the wiping member 9, the wiping member 9 impacts the pH sensor 5. In this embodiment, the collision distance between the wiping member 9 and the pH sensor 5 is short and due to water resistance, the impact force of the wiping member 9 on the pH sensor 5 is small and will not cause damage to the pH sensor 5. The wiping member 9 causes the water to fluctuate. With the impact and wiping action of the wiping member 9 and the pH sensor 5 and the impact of the water fluctuation, the solution adhering to the pH sensor 5 is greatly accelerated to fall off.

[0045] The acceleration assembly includes a storage tank 20, an acceleration tube 21, a solenoid valve 22, a phase change ball 23, and a power unit for driving the phase change ball 23. The phase change ball 23 contains a phase change material with reversible heat absorption and release. The storage tank 20 is installed on the inner wall of the fermenter 1. One end of the acceleration tube 21 is connected to the storage tank 20, and the other end is connected to the detection cylinder 3. The length of the acceleration tube 21 is sufficient for the movement of the detection cylinder 3, and the acceleration tube 21 is made of a soft material. The storage tank 20 is filled with a cleaning agent for cleaning the pH sensor 5. An acceleration pump connected to the acceleration tube 21 can also be installed in the storage tank 20 to accelerate the entry of the cleaning agent into the storage tank 20. The storage tank 20 is provided with a phase change hole 24. The storage tank 20 is placed vertically, and the phase change hole 24 is horizontally oriented. During pH adjustment in fermentation tank 1, an acid-base neutralization reaction occurs, generating heat. At this time, the phase change ball 23 begins to absorb heat. When the phase change ball 23 moves into the phase change hole 24, in order to accelerate the heat release rate of the phase change ball 23, the storage tank 20 is made of a material with a high conductivity at the position of the phase change hole 24. Moreover, the initial position of the phase change ball 23 is closer to the solution, so the heat absorption efficiency of the phase change ball 23 is greatly enhanced compared to the heat absorption of the storage tank 20 itself. When the phase change ball 23 is in the position of the phase change hole 24, it begins to release heat rapidly, which heats the cleaning agent in the storage tank 20. Preheating the cleaning agent to a suitable temperature can improve the activity of the cleaning agent, accelerate the dissolution and removal of dirt, and thus shorten the cleaning time.

[0046] Reference Figures 2-5The power unit includes a first pipe 25, a second pipe 26, a third pipe 27, an elastic rod 28, and a push rod 29. The bottom end of the first pipe 25 is closed. The elastic rod 28 is elastically positioned at the bottom end of the first pipe 25. The second pipe 26 is arc-shaped, with one end connected to one end of the first pipe 25 and the other end facing the opening of the phase change hole 24. One end of the third pipe 27 faces the phase change hole 24, and the other end is connected to the bottom end of the first pipe 25. The push rod 29 is slidably mounted on the storage tank 20. The first pipe 25 and the second pipe 26 are connected to the storage tank 20. The inner diameter of pipes 6 is larger than that of phase change ball 23. Furthermore, the upper sidewalls of the second pipe 26 and the third pipe 27, near the phase change hole 24, extend towards the phase change hole 24 to prevent the phase change ball 23 from sliding out of the first pipe 25 and falling off the storage tank 20, thus facilitating the guidance of the phase change ball 23 into the phase change hole 24. In this embodiment, the elastic rod 28 is driven electrically. A power electric actuator 30 is fixedly connected to the first pipe 25 near the elastic rod 28, and a power electromagnet 3 is fixedly connected to the output end of the power electric actuator 30. 2. The power electromagnet 32 ​​attracts the elastic rod 28. A handle 31 is fixedly connected to the outer wall of the elastic rod 28. A power spring 33 is sleeved on the outer wall of the elastic rod 28. The two ends of the power spring 33 are fixedly connected to the handle 31 and the bottom end of the first pipe 25, respectively. The power electromagnet 32 ​​is used to attract the elastic rod 28, and the power electric push rod 30 pulls the elastic rod 28, stretching and deforming the power spring 33. When the power electromagnet 32 ​​is de-energized, under the elastic force of the power spring 33, the elastic rod 28 moves the phase change ball 23 toward the second pipe. The phase change ball 23 falls into the phase change hole 24 as the phase change ball moves in the 26 direction, while the push rod 29 moves horizontally. A push electric push rod is fixedly connected to the other side of the storage tank 20. The push electric push rod is fixedly connected to the push rod 29 and can push the phase change ball 23 out of the phase change hole 24. Since the extension of the second pipe 26 and the third pipe 27 restricts the phase change ball 23, the phase change ball 23 falls into the third pipe 27 under its own gravity and then returns to the bottom of the first pipe 25 through the third pipe 27.

[0047] Reference Figures 6-7The control mechanism includes a control frame 35 rotatably mounted outside the fermentation tube, a top box 36, a bottom box 37, a liquid level sensor 38, a regulating pipe 39, and a regulating component for controlling the flow rate within the regulating pipe 39. The regulating pipe 39 is a flexible hose, and is in a taut state. The liquid level sensor 38 is installed inside the bottom box 37. The top box 36 and the bottom box 37 are fixedly connected to the control frame 35. The regulating pipe 39 connects the top box 36 and the bottom box 37. The size of the top box 36 can be larger than the size of the bottom box 37 to ensure that the bottom box 37 is filled with water. The regulating component includes an adjusting block 40, an adjusting rod 41, an adjusting wheel 42, and a regulating component for controlling the flow rate within the regulating pipe 39. The adjusting member 43 controls the movement of the adjusting rod 41. The adjusting block 40 is provided with a through hole 44 for the adjusting tube 39 to pass through. The adjusting block 40 is also provided with an adjusting groove 45. The adjusting groove 45 is inclined, while the through hole 44 is vertical. The length direction of the through hole 44 is not horizontal and intersects with the length direction of the adjusting groove 45. Both sides of the adjusting groove 45 are provided with inclined grooves 46. The inclined grooves 46 are parallel to the length direction of the adjusting groove 45. The two ends of the adjusting rod 41 slide in the two inclined grooves 46 respectively. The adjusting wheel 42 is rotatably disposed outside the adjusting rod 41 and is located in the adjusting groove 45. When the adjusting rod 41 is located at both ends of the inclined groove 46, the two ends of the adjusting tube 39 are at their maximum or minimum.

[0048] In this embodiment, when the top box 36 is at the top, as the adjusting wheel 42 and the adjusting rod 41 move downwards, the distance between the peripheral wall of the adjusting wheel 42 and the bottom of the adjusting groove 45 gradually decreases, meaning the adjusting wheel 42 is squeezing the adjusting tube 39. When the adjusting wheel 42 is at the lowest end of the adjusting groove 45, the adjusting tube 39 is completely squeezed and deformed, at which point the adjusting tube 39 is blocked. In this embodiment, the adjusting component 43 drives the adjusting rod 41. The transmission part includes an adjusting electric push rod, which is fixed on the adjusting block 40. The movement direction is set along the length of the inclined groove 46. The adjusting electric actuator is fixedly connected to either end of the adjusting rod 41. Therefore, the adjusting electric actuator can push the adjusting rod 41 to reciprocate along the inclined groove 46. At this time, the adjusting wheel 42 rolls under the frictional force with the adjusting tube 39 and moves with the adjusting rod 41, thereby achieving the effect of controlling the flow of the adjusting tube 39. In the initial state, the adjusting electric actuator pushes the adjusting rod 41 to the lowest end of the inclined groove 46. At this time, the adjusting tube 39 is blocked. When the pH adjustment is in the early stage in the fermenter 1, it is necessary to shorten the measurement time. The time interval is such that the cleaning effect of pH sensor 5 needs to be accelerated. At this time, the adjusting rod 41 needs to be pulled to the top of the adjusting tank 45. At this time, the adjusting tube 39 is fully open. In this embodiment, this time can be set as the time for cleaning pH sensor 5 with clean water. When the liquid level sensor 38 detects that the water level in the bottom tank 37 has risen to the top, the phase change ball 23 is immediately controlled to move into the phase change hole 24. The cleaning agent in the storage tank 20 is heated, and the accelerating tube 21 is controlled to squeeze the cleaning agent into the storage tank 20. At this time, and through two wiping The repeated agitation of the wiping element 9 greatly accelerates the mixing effect of the cleaning agent, thus greatly speeding up the cleaning efficiency of the pH sensor 5. The water and cleaning agent mixture after cleaning can be pumped away, and the next testing task can begin. Finally, the top tank 36 and the bottom tank 37 need to be swapped, and the flow rate of the regulating pipe 39 should be fully opened to quickly flow the water in the bottom tank 37 into the top tank 36, which will start the timing for the next cleaning work. In the middle and late stages of pH adjustment in the fermentation tank 1, it is only necessary to move the regulating rod 41 and the regulating wheel 42 to the middle position of the regulating tank 45.

[0049] The implementation principle of the preparation device for the avermectin-streptomyces-purpureus compound microbial agent in this application embodiment is as follows: After the drive motor 16 controls the drive gear 17 to rotate, the drive gear 17 controls the ring gear 18 to rotate, and the ring gear 18 drives the protective cylinder 4 to rotate, thereby achieving the rotation effect of the protective cylinder 4. The compound microbial agent is then applied, and the compound microbial agent is detected by the pH sensor 5 until the pH value meets the requirements. After the detection is completed, the pH sensor 5 needs to be cleaned. Water is injected into the detection cylinder 3, and the water surface does not need to completely submerge the pH sensor 5. The wiping element 9 can absorb water. Through the reciprocating motion of the wiping element 9, the wiping element 9 impacts the pH sensor 5. In this embodiment, the collision distance between the wiping element 9 and the pH sensor 5 is short, and due to water resistance, the impact force of the wiping element 9 on the pH sensor 5 is small and will not cause damage to the pH sensor 5. The wiping element 9 causes the water to fluctuate. With the impact and wiping action of the wiping element 9 and the pH sensor 5 and the impact of the water fluctuation, the solution adhering to the pH sensor 5 is greatly accelerated to fall off.

[0050] To accelerate cleaning efficiency, a power electromagnet 32 ​​is used to attract the elastic rod 28, and the elastic rod 28 is pulled by a power electric push rod 30. The power spring 33 is stretched and deformed. When the power electromagnet 32 ​​is de-energized, under the elastic force of the power spring 33, the elastic rod 28 moves the phase change ball 23 toward the second pipe 26. The phase change ball 23 falls into the phase change hole 24, and the phase change ball 23 begins to rapidly release heat, which heats the cleaning agent in the storage tank 20. Preheating the cleaning agent to a suitable temperature can improve the activity of the cleaning agent, accelerate the dissolution and removal of dirt, and thus shorten the cleaning time. The repeated stirring of the two wiping parts 9 greatly accelerates the mixing effect of the cleaning agent, thus greatly accelerating the cleaning time of the pH sensor 5. The water and cleaning agent mixture after cleaning can then be pumped away, and the next testing task can begin.

[0051] In the early stage of pH adjustment in fermenter 1, it is necessary to shorten the measurement time interval, so it is necessary to accelerate the cleaning effect of pH sensor 5. At this time, the adjusting rod 41 needs to be pulled to the top of the adjusting tank 45. At this time, the adjusting tube 39 is fully open. In this embodiment, this time can be set as the time for cleaning pH sensor 5 with clean water. When the liquid level sensor 38 detects that the water level in the bottom tank 37 has risen to the top, the phase change ball 23 is immediately controlled to move into the phase change hole 24. The cleaning agent in the storage tank 20 is heated. Through the repeated stirring of the two wiping parts 9, the cleaning agent mixing effect is greatly accelerated, which greatly accelerates the cleaning efficiency of pH sensor 5. Finally, the top tank 36 and the bottom tank 37 need to be swapped. The adjusting tube 39 is fully opened to quickly flow the water in the bottom tank 37 into the top tank 36 to start the timing for the next cleaning work. In the middle and late stages of pH adjustment in fermenter 1, it is only necessary to move the adjusting rod 41 and the adjusting wheel 42 to the middle position of the adjusting tank 45.

[0052] Example 2

[0053] This application discloses a method for preparing an avermectin-streptomyces-purpureus compound inoculant. (Refer to...) Figure 1 The preparation method of the avermectin-streptomyces-purpureus compound inoculant includes the following steps:

[0054] S1. Strain preparation: Take out Streptomyces avermectin, Paecilomyces lilacinus, and Bacillus brevis, ensuring that the strains have good activity; ensure the purity and activity of Streptomyces avermectin, and perform rejuvenation and screening if necessary; check the biological characteristics and functions of Paecilomyces lilacinus to ensure that it has the expected control effect; obtain strains of Bacillus brevis, ensuring that they have good growth and reproduction capabilities.

[0055] S2. Culture Medium Preparation: Optimize the culture medium formula and conditions through experiments to improve the yield and activity of the compound bacteria; prepare a basic culture medium suitable for the co-growth of the three microorganisms. This culture medium should contain appropriate amounts of carbon source, nitrogen source, inorganic salts, and necessary trace elements. It can be appropriately adjusted according to the nutritional requirements of each strain to achieve the best compound fermentation effect. Considering the requirement of avermectin streptomyces for avermectin synthesis, specific precursor substances or inducers can be added to the culture medium.

[0056] S3: Compound Fermentation: Inoculation: Inoculate the prepared Streptomyces avermectin, Paecilomyces lilacinus and Bacillus retroflexus strains into the basal culture medium in a certain proportion; the inoculation amount should be adjusted according to the growth rate and activity of each strain to ensure that they can grow in a coordinated manner during the compound fermentation process;

[0057] S4: Fermentation Condition Control: Control parameters such as temperature, pH, and dissolved oxygen during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; the pH value is monitored and controlled in real time using a detection device; during the fermentation process, the compound microbial agent is sampled and tested at any time through detection tube 3, and the pH value of the compound microbial agent is adjusted in real time to ensure fermentation conditions. In addition, the time interval for pH value detection needs to be intelligently adjusted and controlled in conjunction with the fermentation process to make reasonable use of monitoring methods, save costs to the greatest extent, and achieve the best pH value adjustment effect.

[0058] S5: Separation and Purification: After fermentation, the bacterial cells and fermentation broth are separated by centrifugation, filtration, and other methods to obtain the compound microbial agent. The compound microbial preparation undergoes bioactivity testing and safety assessment to ensure its effectiveness and safety in practical applications. This compound microbial preparation is then promoted for application in agriculture, horticulture, and other fields to leverage its broad-spectrum, highly efficient, safe, and long-lasting biological control effects.

[0059] Example 3

[0060] This application discloses an avermectin-streptomyces-purpureus compound bacterial agent. (Refer to...) Figure 1 The avermectin-streptomyces-purpureus compound microbial agent includes avermectin-streptomyces, purpureus, and Bacillus laterosporus. Other adjuvants may be needed to adjust the biocompound properties when necessary. This compound combines the advantages of the three microorganisms, enabling broader control of various pests, nematodes, and pathogens. Synergistic effects may exist between different microorganisms, jointly enhancing control efficacy. For example, purpureus and Bacillus laterosporus may inhibit the growth of nematodes and pathogens through different mechanisms. The use of the compound can reduce the resistance problems caused by continuous use of single-microbial agents. Finally, both purpureus and Bacillus laterosporus produce physiologically active substances that promote plant growth; the use of the compound may more significantly promote plant growth and development. The avermectin-streptomyces, purpureus, and Bacillus laterosporus compound has broad application prospects and significant advantages in agriculture.

[0061] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A device for preparing an abamectin-streptomyces-purpureus compound inoculant, characterized in that: It includes a strain preparation module, a culture medium preparation module, a compound fermentation module and a centrifugation purification module. The compound fermentation module includes a fermenter (1), a detection mechanism for detecting compound microbial agents, a control mechanism for controlling the measurement time interval and a cleaning mechanism. The detection mechanism includes a detection frame (2) that is lifted and installed inside the fermenter (1), multiple detection cylinders (3) fixed on the detection frame (2), a protective cylinder (4) that is rotatably installed outside the detection cylinders (3), a pH sensor (5) installed inside the detection cylinders (3), and a drive assembly for controlling the rotation of the protective cylinders (4). The detection cylinders (3) are provided with detection holes (6), and the protective cylinders (4) are provided with protective holes (7). When the protective cylinders (4) are in their initial position, the inner wall of the protective cylinders (4) blocks the detection holes (6). The cleaning mechanism is used to clean the pH sensor (5). The cleaning mechanism includes a cleaning rack (8) that is lifted and installed inside the protective cylinder (4), a cleaning component for cleaning the pH sensor (5), an acceleration component for accelerating the cleaning of the pH sensor (5), and a circulation component for realizing the circulation of water inside the detection cylinder (3). The acceleration assembly includes a storage tank (20), an acceleration tube (21), a solenoid valve (22), a phase change ball (23), and a power unit for driving the phase change ball (23) to move. The storage tank (20) is installed on the inner wall of the fermentation tank (1). One end of the acceleration tube (21) is connected to the storage tank (20), and the other end is connected to the detection cylinder (3). The storage tank (20) is filled with a cleaning agent for cleaning the pH sensor (5). The storage tank (20) is provided with a phase change hole (24), which is used to place the phase change ball (23). The phase change ball (23) is filled with phase change material. The control mechanism includes a control frame (35) rotatably mounted outside the fermenter (1), a top box (36), a bottom box (37), a liquid level sensor (38), a regulating pipe (39), and a regulating component for controlling the flow rate in the regulating pipe (39). The regulating pipe (39) is a flexible hose and is in a taut state. The liquid level sensor (38) is installed inside the bottom box (37). The top box (36) and the bottom box (37) are fixedly connected to the control frame (35). The regulating pipe (39) is used to connect the top box (36) and the bottom box (37). The adjustment assembly includes an adjustment block (40), an adjustment rod (41), an adjustment wheel (42), and an adjustment component (43) for controlling the movement of the adjustment rod (41) fixed on the control frame (35). The adjustment block (40) is provided with a through hole (44) for the adjustment tube (39) to pass through. The adjustment block (40) is also provided with an adjustment groove (45). The adjustment groove (45) is inclined. Both sides of the adjustment groove (45) are provided with inclined grooves (46). The two ends of the adjustment rod (41) slide in the two inclined grooves (46) respectively. The adjustment wheel (42) is rotatably disposed outside the adjustment rod (41) and located inside the adjustment groove (45). When the adjustment rod (41) is located at both ends of the inclined grooves (46), the flow rate of the adjustment tube (39) is at its maximum or minimum.

2. The apparatus for preparing the avermectin-streptomyces-purpureus compound inoculant according to claim 1, characterized in that: The cleaning assembly includes two wiping elements (9) elastically disposed on the cleaning rack (8) and a drive unit for realizing the elastic movement of the two wiping elements (9), the two wiping elements (9) being located on both sides of the pH sensor (5).

3. The apparatus for preparing the avermectin-streptomyces-purpureus compound inoculant according to claim 2, characterized in that: The drive unit includes two slide plates (10), two first springs (11), two first electromagnets (12), and two drive members (34). The wiping member (9) is fixed on the slide plate (10). The two first springs (11) are used to make the two slide plates (10) elastically set on the cleaning rack (8). The drive members (34) are used to control the reciprocating motion of the first electromagnets (12). The first electromagnets (12) are used to attract the slide plates (10).

4. The apparatus for preparing the avermectin-streptomyces-purpureus compound inoculant according to claim 3, characterized in that: The power unit includes a first pipe (25), a second pipe (26), a third pipe (27), an elastic rod (28), and a push rod (29). The bottom end of the first pipe (25) is closed. The elastic rod (28) is elastically disposed at the bottom end of the first pipe (25). The second pipe (26) is arc-shaped and one end is connected to one end of the first pipe (25), while the other end is directly opposite the opening of the phase change hole (24). One end of the third pipe (27) is directly opposite the phase change hole (24), while the other end is connected to the bottom end of the first pipe (25). The push rod (29) is slidably disposed on the storage tank (20).

5. A method for preparing an avermectin-streptomyces-purpureus compound microbial agent, based on the apparatus for preparing the avermectin-streptomyces-purpureus compound microbial agent according to claim 4, characterized in that: Includes the following steps: S1. Preparation of bacterial strains: Take out Streptomyces ivermectin, Paecilomyces lilacinus, and Bacillus breviculatus, and ensure that the bacterial strains have good activity; S2. Culture medium preparation: Optimize the formula and conditions of the culture medium through experiments to improve the yield and activity of the compound bacteria; S3: Compound fermentation: Inoculation: Inoculate the prepared Streptomyces avermectin, Paecilomyces lilacinus and Bacillus retroflexus strains into the basic culture medium in a certain proportion; S4: Fermentation condition control: Controlling parameters such as temperature, pH value, and dissolved oxygen during the fermentation process to create an environment suitable for the co-growth of the three microorganisms; wherein the detection mechanism in claim 1 is used to monitor and control the pH value in real time. S5: Separation and purification: After fermentation, the bacterial cells and fermentation broth are separated by centrifugation, filtration and other methods to obtain the compound bacterial agent.

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