Full-automatic all-in-one machine for NGS library establishment, hybridization sequencing and interpretation

Abstract

The utility model relates to a full-automatic all-in-one machine for NGS library building hybridization sequencing interpretation, which is used for sample adding and sequencing of a sequencing chip, and comprises a rack, a manipulator, a library preparation instrument and a sequencing device, the sequencing device comprises a gene sequencer, a sample adding clamping mechanism and a turnover mechanism, the library preparation instrument is used for sample adding of the sequencing chip, and the sample adding clamping mechanism is used for sample adding of the sequencing chip. The sample adding and clamping mechanism comprises a clamping tool and a clamping base, the clamping base is provided with a second positioning groove, the clamping tool is placed in the second positioning groove, the clamping tool clamps the sequencing chip, the overturning mechanism overturns the clamping tool to drive the sequencing chip for sample adding to overturn, the manipulator comprises an execution end and a clamping piece, the clamping piece is arranged at the execution end, and the clamping piece is arranged at the execution end. The clamping piece clamps the clamping tool, and the execution end moves among the library preparation instrument, the turnover mechanism and the gene sequencer, so that the sequencing chip is inserted into a preset position of the slot after being subjected to sample adding and turnover. The clamping tool clamps the sequencing chip, so that the sequencing chip is prevented from being overturned and falling off, automatic insertion of the sequencing chip is realized, and the sequencing efficiency is improved.

Description

Technical Field

[0001] This utility model relates to the field of microbiology instrumentation technology, and in particular to a fully automated integrated machine for NGS library construction, hybridization, sequencing and interpretation. Background Technology

[0002] Next-generation sequencing (NGS), also known as high-throughput sequencing or second-generation sequencing, is characterized by its ability to sequence hundreds of thousands to millions of DNA molecules in parallel at once and generally shorter read lengths.

[0003] The high-throughput sequencing process includes the following steps: sample preparation, library preparation, sequencing reaction, and data analysis. In the sequencing reaction step, the sequencing chip needs to be manually loaded with the sample, flipped, and then inserted into the gene sequencer's slot for DNA sequencing. Especially since library preparation is typically completed around 3 AM on production lines, specially assigned personnel are required to perform sequencing, placing a significant strain on staff scheduling and the efficiency of testing institutions. This results in low sequencing efficiency and issues such as chip dislodgement and chip jamming.

[0004] Therefore, there is an urgent need for a fully automated integrated machine for NGS library preparation, hybridization, sequencing, and interpretation to solve the above-mentioned technical problems. Utility Model Content

[0005] The purpose of this invention is to propose a fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation, so as to solve at least one of the above-mentioned problems.

[0006] To achieve the above objectives, this utility model provides a fully automated integrated machine for NGS library preparation, hybridization, sequencing, and interpretation, used for sample loading and sequencing of sequencing chips. It includes a rack, a robotic arm mounted on the rack, a library preparation instrument, and a sequencing device. The sequencing device includes a gene sequencer, a sample loading clamping mechanism, and a flipping mechanism. The library preparation instrument is capable of loading samples onto the sequencing chip. The gene sequencer has slots for inserting sequencing chips.

[0007] The sample loading clamping mechanism includes a clamping fixture and a clamping base. The clamping base is provided with a second positioning groove. The clamping fixture is placed in the second positioning groove. The clamping fixture has an open state and a closed state. When the clamping fixture comes into contact with the gene sequencer, it will trigger the clamping fixture to switch from the closed state to the open state. When the clamping fixture is in the closed state, the clamping fixture clamps the sequencing chip.

[0008] A flipping mechanism is provided, which can flip the clamping fixture to flip the sequencing chip after sample loading.

[0009] The robotic arm includes an actuator and a gripper. The gripper is capable of gripping the gripping fixture or the gripping base. The actuator is capable of moving between the library preparation instrument, the flipping mechanism, and the gene sequencer, so that the sequencing chip is sampled, flipped, and inserted into a preset position in the slot.

[0010] Furthermore, the clamping fixture includes:

[0011] A fixing component includes a fixing member and a locking positioning member. The fixing member has a mounting groove, and the locking positioning member is disposed in the mounting groove. The locking positioning member has a positioning part for positioning the sequencing chip.

[0012] The top cover, one end of which is rotatably connected to the fixing member, is used to switch between opening or closing the mounting slot;

[0013] A reset component is disposed between the upper cover and the fixing component, the reset component being used to automatically open the unlocked upper cover;

[0014] A locking assembly is disposed between the upper cover and the fixing assembly, the locking assembly being used to lock the closed upper cover;

[0015] When the top cover is closed, the sequencing chip is held between the locking positioning element and the top cover.

[0016] Furthermore, the locking component includes:

[0017] A first locking connector is disposed on the upper cover;

[0018] A second locking connector is disposed on the fixing component. The first locking connector and the second locking connector can be engaged or disengaged. When the second locking connector contacts the gene sequencer, the first locking connector and the second locking connector are disengaged.

[0019] Furthermore, the first locking connector includes a hook, one end of which is connected to the upper cover;

[0020] The second locking connector includes a hook connector. A sliding groove is provided on the fixing member below the locking positioning member, and one end of the hook connector is slidably disposed in the sliding groove.

[0021] The hook connector has a slot. When the second locking connector contacts the gene sequencer, one end of the hook connector slides in the sliding groove so that the other end of the hook disengages from the slot.

[0022] Furthermore, the locking assembly also includes a locking spring, which is used to keep the hook and the slot in a locked state. One end of the hook connector is connected to one end of the locking spring, and the other end of the locking spring is connected to the wall of the sliding groove.

[0023] Furthermore, the upper cover and the fixing member are rotatably connected via a pivot, and the reset assembly includes:

[0024] A torsion spring mounting component is rotatably mounted on the rotating shaft, and the torsion spring mounting component is connected to the upper cover;

[0025] A torsion spring, which is sleeved on the rotating shaft and installed on the torsion spring mounting component.

[0026] Furthermore, the sample clamping mechanism also includes a clamping base and a sample feeding seat, wherein a second positioning groove is provided on the clamping base, and the clamping fixture is placed in the second positioning groove;

[0027] The sequencing chip is provided with a sample dispensing port, the sample dispensing holder is located on one side of the sample dispensing port, and the clamping fixture is located on the opposite side of the sequencing chip;

[0028] The sequencing chip has a side positioning protrusion on the side wall of the end that extends out of the clamping fixture;

[0029] The sample loading seat is placed on the clamping base, and the sample loading seat is provided with an open slot. The side positioning protrusion is engaged in the open slot.

[0030] Furthermore, the robotic arm also includes a chip pushing mechanism, which is connected to the actuator and used to push the stuck sequencing chip to a preset position in the slot. The chip pushing mechanism includes:

[0031] Push the base;

[0032] A Z-axis adjustment assembly, disposed on one side of the push base, includes a Z-axis moving component movable along the Z-direction; and

[0033] A pushing component, fixedly disposed on the side of the Z-axis moving component away from the pushing base, the pushing component comprising:

[0034] A pushing surface is located on the side of the pushing component away from the Z-direction moving component, and the pushing surface is perpendicular to the X-direction;

[0035] A first actuating block, the first actuating block extending from one end of the pushing surface in the Z direction along the X direction and away from the Z-direction moving member; and

[0036] The second actuating block extends from the pushing surface at the other end in the Z direction along the X direction and away from the Z-direction moving member.

[0037] Furthermore, the flipping mechanism includes:

[0038] A flip drive assembly includes a rotary drive component and a clamping drive component connected to each other. The rotary drive component is configured to drive the clamping drive component to rotate about a rotation axis. The clamping drive component has a first actuating end and a second actuating end, and at least one of the first actuating end and the second actuating end is configured to be movable between a clamping position and a releasing position.

[0039] A first gripper, the first gripper being disposed at the first execution end; and

[0040] The second gripper is disposed at the second actuating end.

[0041] A clamping area is formed between the first gripper and the second gripper. A first clamping positioning element is provided on the side of the first gripper facing the clamping area, and a second clamping positioning element is provided on the side of the second gripper facing the clamping area.

[0042] Furthermore, the fully automated NGS library preparation, hybridization, sequencing, and interpretation system also includes a low-temperature storage mechanism, which includes:

[0043] An anti-condensation component includes a storage mounting base, a pressure cap component, and a spring-loaded component. The storage mounting base has a mounting surface, the pressure cap component is mounted on the mounting surface, and a first end of the spring-loaded component is mounted on the mounting surface. The pressure cap component has a first state and a second state. When the pressure cap component is in the first state, both the second ends of the pressure cap component and the spring-loaded component abut against the sealing surface of the consumable box. When the pressure cap component is in the second state, the pressure cap component is away from the sealing surface of the consumable box, and the second end of the spring-loaded component extends beyond the pressure cap component and abuts against the sealing surface of the consumable box.

[0044] A refrigeration assembly includes a support frame and a refrigeration component, wherein a consumable box is placed on the support frame and the refrigeration component cools the consumable box.

[0045] A storage drive assembly that drives the storage mounting base to move up and down relative to the support frame.

[0046] The beneficial effects of this utility model are as follows:

[0047] This utility model provides a fully automated integrated machine for NGS library preparation, hybridization, sequencing, and interpretation, used for sample loading and sequencing of sequencing chips. It includes a frame and a robotic arm mounted on the frame, a sequencing device, and a library preparation instrument. The sequencing device includes a gene sequencer, a sample loading clamping mechanism, and a flipping mechanism. The library preparation instrument can load samples onto the sequencing chips. The gene sequencer has slots for inserting sequencing chips. The sample loading clamping mechanism includes a clamping fixture and a clamping base. The clamping base has a second positioning groove, and the clamping fixture is placed in the second positioning groove. The clamping fixture is in an open state. In the closed state, when the clamping fixture comes into contact with the gene sequencer, it will trigger the clamping fixture to switch from the closed state to the open state. When the clamping fixture is in the closed state, the clamping fixture clamps the sequencing chip. The flipping mechanism can flip the clamping fixture to drive the sequencing chip after sample loading to flip. The robot includes an execution end and a clamping component. The clamping component is located at the execution end. The clamping component can grip the clamping fixture or the clamping base. The execution end can move between the library preparation instrument, the flipping mechanism and the gene sequencer so that the sequencing chip is loaded, flipped and inserted into the preset position of the slot. The robotic arm first grasps the sample loading clamp and moves it into the library preparation instrument for sample loading. Then, the robotic arm grasps the clamping base and moves it to the flipping mechanism. The clamping base moves the loaded sequencing chip, along with the clamping fixture, to the flipping mechanism as well. The flipping mechanism grasps the clamping fixture, separating it from the clamping base. The flipping mechanism then flips the clamping fixture, causing the loaded sequencing chip to flip along with it. The robotic arm then moves the flipped clamping fixture and sequencing chip to the gene sequencer. Through the movement of the robotic arm's actuator, the sequencing chip is inserted into its slot. The clamping fixture firmly holds the sequencing chip, and the flipping mechanism flips the clamping fixture along with the sequencing chip, preventing the sequencing chip from falling out during the flipping process. The sequencing device, robotic arm, and library preparation instrument work together to achieve automated sequencing chip insertion, eliminating the need for manual sequencing by specially assigned personnel and reducing staffing schedules, thus improving sequencing efficiency. Attached Figure Description

[0048] Figure 1 This is a top view of the fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation provided in this embodiment of the utility model;

[0049] Figure 2 This is a schematic diagram of the structure of the fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation provided in this embodiment of the utility model;

[0050] Figure 3 This is a schematic diagram of a portion of the structure of the robotic arm and sequencing device provided in this embodiment of the utility model;

[0051] Figure 4 This is a schematic diagram of the clamping fixture provided in the embodiment of the present invention in the closed state;

[0052] Figure 5This is a schematic diagram of the clamping fixture provided in this embodiment of the present invention at a first angle in the open state;

[0053] Figure 6 This is a schematic diagram of the clamping fixture provided in this embodiment of the present invention at a second angle in the open state;

[0054] Figure 7 This is a cross-sectional view of the clamping fixture provided in this embodiment of the utility model;

[0055] Figure 8 This is a schematic diagram of the positioning component provided in an embodiment of the present utility model;

[0056] Figure 9 This is a schematic diagram of the sequencing chip provided in this embodiment of the present invention from a first angle.

[0057] Figure 10 This is a schematic diagram of the sequencing chip provided in this embodiment of the present invention from a second angle.

[0058] Figure 11 This is a schematic diagram of the sample clamping mechanism provided in this embodiment of the utility model;

[0059] Figure 12 This is a schematic diagram of the structure of the clamping base provided in this embodiment of the utility model;

[0060] Figure 13 This is a schematic diagram of the structure of the sample clamping mechanism for removing the sample holder provided in this embodiment of the utility model;

[0061] Figure 14 This is a schematic diagram of the first angle of the sample feeding seat provided in this embodiment of the utility model;

[0062] Figure 15 This is a schematic diagram of the second angle of the sample feeding holder provided in this embodiment of the utility model;

[0063] Figure 16 This is a cross-sectional view of the sample feeding holder provided in an embodiment of this utility model;

[0064] Figure 17 This is a schematic diagram of the gene sequencer, sequencing chip, and chip driving mechanism provided in an embodiment of the present invention;

[0065] Figure 18 This is a schematic diagram of the chip pushing mechanism provided in an embodiment of the present utility model;

[0066] Figure 19 This is an exploded view of the chip pushing mechanism provided in this embodiment of the utility model;

[0067] Figure 20This is a cross-sectional view of the push base and X-axis buffer assembly provided in this embodiment of the utility model;

[0068] Figure 21 This is a perspective view of the flipping mechanism provided in an embodiment of the present utility model, wherein the clamping fixture is located between the first jaw and the second jaw;

[0069] Figure 22 This is a right view of the flipping mechanism provided in this embodiment of the utility model;

[0070] Figure 23 This is a perspective view of a flipping mechanism provided in another embodiment of the present invention;

[0071] Figure 24 This is a schematic diagram of the anti-condensation component provided in this embodiment of the utility model;

[0072] Figure 25 This is an exploded view of the anti-condensation component provided in this embodiment of the utility model;

[0073] Figure 26 This is a cross-sectional view of a portion of the anti-condensation component provided in this embodiment of the utility model;

[0074] Figure 27 Yes, yes Figure 26 A magnified view of a section at point A in the middle;

[0075] Figure 28 This is a schematic diagram of the structure of the spring-damping component provided in this embodiment of the utility model;

[0076] Figure 29 This is a schematic diagram of the structure of the fixing base provided in an embodiment of the present utility model;

[0077] Figure 30 This is a schematic diagram of the structure of the low-temperature storage mechanism provided in this embodiment of the utility model;

[0078] Figure 31 This is a schematic diagram of a portion of the structure of the low-temperature storage mechanism provided in this embodiment of the utility model;

[0079] Figure 32 This is a schematic diagram of another angle of the low-temperature storage mechanism provided in this embodiment of the utility model.

[0080] In the picture:

[0081] 1. Rack;

[0082] 2. Robotic arm; 21. Actuating end; 22. Gripping component; 23. Chip pushing mechanism; 231. Pushing base; 2311. X-axis channel; 232. Z-axis adjustment component; 2321. Z-axis moving component; 2322. Z-axis mounting base; 23221. First mounting block; 23222. Second mounting block; 2323. Z-axis guide component; 2324. Z-axis elastic component; 233. Pushing component; 2331. Pushing surface; 2332. First actuating block; 23321. First actuating surface; 2333. Second actuating block; 23331. Second actuating surface; 234. X-axis buffer component; 2341. X-axis moving component; 2342. X-axis guide component; 2343. X-axis elastic component;

[0083] 3. Sequencing device;

[0084] 31. Gene sequencer; 311. Slot;

[0085] 32. Sequencing chip; 321. End positioning protrusion; 322. Sample dispensing port; 323. Side positioning protrusion;

[0086] 33. Sample clamping mechanism; 330. Clamping fixture; 331. Fixing component; 3310. Mounting groove; 3311. Fixing component; 33111. Clamping hole; 33112. Sliding groove; 33113. Locking cavity; 3312. Locking positioning component; 33121. Positioning part; 33122. First arc surface; 33123. First positioning groove; 33124. Locking clearance groove; 332. Top cover; 333. Reset component; 3331. Torsion spring mounting component; 3332. Torsion spring; 334. Locking component; 3341. First locking connector; 3411, Hook; 3342, Second locking connector; 33421, Hook connector; 334211, Slot; 334212, Second arc surface; 334213, Limiting hole; 3343, Locking spring; 3345, Limiting component; 336, Clamping base; 3361, Second positioning slot; 3362, First observation window; 3363, Second observation window; 3364, Positioning wall; 3365, Weight reduction hole; 337, Sample feeding seat; 3371, Opening slot; 3372, Sample feeding channel; 3373, Opening positioning slot; 338, Rotating shaft;

[0087] 34. Flipping mechanism; 341. Flipping fixing base; 3411. Mounting hole; 342. Flipping drive assembly; 3421. Rotation drive component; 3422. Clamping drive component; 34221. First actuating end; 34222. Second actuating end; 3423. Rotation axis; 343. First gripper; 344. Second gripper; 3441. Second clamping positioning component; 345. Connecting assembly; 3451. First flipping connector; 3452. Second flipping connector; 346. Sensor assembly; 3461. Slotted photoelectric switch; 3462. Switch baffle; 3463. Switch mounting base;

[0088] 4. Library preparation instrument; 41. Conveyor module; 42. Inlet / outlet;

[0089] 5. Storage device; 51. Room temperature storage mechanism;

[0090] 52. Low-temperature storage mechanism;

[0091] 521. Anti-condensation component; 5211. Storage mounting base; 52111. Mounting part; 521111. Fixing groove; 521112. First guide groove; 521113. Mounting surface; 52112. Connecting part; 5212. Pressure cap component; 52121. Pressure plate; 521211. First clearance groove; 52122. Elastic pad; 521221. Second clearance groove; 5213. Softening component; 52131. Softening element; 521311. Guide part; 521312. Top pressure part; 5213 2. Soft-close fixing seat; 521321, movable groove; 521322, second guide groove; 521323, fastening groove; 521324, fixing part; 521325, protrusion; 52133, elastic element; 5214, buffer element; 5215, fastener; 522, storage drive assembly; 5221, storage drive board; 5222, storage drive component; 5223, mounting plate; 5224, connecting block; 523, cooling assembly; 5231, support frame; 5232, cooling component; 524, housing;

[0092] 6. Consumables box;

[0093] 7. Quantitative fluorescence detection device;

[0094] 8. Calibration device;

[0095] 9. Sealing device. Detailed Implementation

[0096] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this utility model and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this utility model are shown in the accompanying drawings, not all of them.

[0097] This utility model defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up", "down", "left", "right", "inner", and "outer", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this utility model.

[0098] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0099] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0100] like Figures 1-32 As shown, this embodiment provides a fully automated integrated machine for NGS (Next-Generation Sequencing, also known as high-throughput sequencing or second-generation sequencing) library preparation, hybridization, sequencing, and interpretation. This machine is used for sample library preparation, sample loading onto sequencing chips (32-bit), automated sequencing, and data analysis to detect genetic diseases, tumor mutations, and pathogenic microorganism infections. The fully automated NGS library preparation, hybridization, sequencing, and interpretation machine provided in this embodiment automatically generates the entire process from sample entry in nucleic acid state to test report generation, without any human intervention.

[0101] like Figures 1-2As shown, this fully automated integrated machine for library construction, hybridization, sequencing, and interpretation includes a frame 1, a robotic arm 2, a sequencing device 3, a library preparation instrument 4, a storage device 5, a quantitative fluorescence detection device 7, a calibration device 8, and a sealing device 9. The robotic arm 2, sequencing device 3, library preparation instrument 4, storage device 5, quantitative fluorescence detection device 7, calibration device 8, and sealing device 9 are all mounted on the frame 1. Materials are stored in the storage device 5. The robotic arm 2 is used to move materials between the sequencing device 3, library preparation instrument 4, storage device 5, quantitative fluorescence detection device 7, calibration device 8, and sealing device 9. The quantitative fluorescence detection device 7 is used for concentration determination, the calibration device 8 is used for position calibration, and the sealing device 9 is used to seal the consumable cartridge 6. The preparation of the sample library is mainly achieved through the robotic arm 2, library preparation instrument 4, storage device 5, quantitative fluorescence detection device 7, calibration device 8, and sealing device 9. The sequencing device 3, robotic arm 2, and library preparation instrument 4 work together to sequence gene fragments from the prepared library samples.

[0102] Specifically, the sequencing device 3 includes a gene sequencer 31, a sequencing chip 32 for sample loading and flipping before being inserted into the gene sequencer 31 for gene fragment sequencing, and a library preparation instrument 4 for sample library preparation and loading of the sequencing chip 32.

[0103] In this embodiment, the consumable cartridge 6, reagent kit, sequencing chip 32, and other equipment used can all be referred to as materials. The equipment used may include the sample loading clamping mechanism 33 described below. The consumable cartridge 6 may store the collected initial sample, intermediate products of the reaction, and final products.

[0104] Furthermore, the storage device 5 includes an ambient temperature storage mechanism 51 and a low temperature storage mechanism 52. The ambient temperature storage mechanism 51 is used to store materials that do not require refrigeration, and the low temperature storage mechanism 52 is used to store materials that require refrigeration.

[0105] Furthermore, such as Figure 3 As shown, the robotic arm 2 can be a multi-axis robotic arm. The robotic arm 2 includes a gripper 22, which is used to grip materials. The gripper 22 can be moved to any position in three-dimensional space so that the robotic arm 2 can transport materials between various devices for inspection.

[0106] Furthermore, the document preparation instrument 4 includes a conveying module 41 and an inlet / outlet 42. The conveying module 41 extends from the inlet / outlet 42 into the document preparation instrument 4. The conveying module 41 is used to input or output materials into or out of the document preparation instrument 4. The inlet / outlet 42 is used for materials to enter and exit the document preparation instrument 4.

[0107] It should be noted that the structure and working principle of the robotic arm 2, the library preparation instrument 4, the fluorescence quantitative detection device 7, the calibration device 8, and the sealing device 9 are existing technologies and can be directly purchased and used. They will not be described in detail in this utility model.

[0108] The specific process for preparing the sample library is as follows:

[0109] Step 1 (Library Preparation): Manually place the consumable cartridge 6 containing the initial sample, along with the reagent kits and empty consumable cartridge 6 required for library preparation, into the library preparation instrument 4. Then, place the reagent kits, consumable cartridge 6, and the sample loading clamp mechanism 33 containing the sequencing chip 32 required for subsequent library preparation steps (e.g., sequencing steps) into the storage device 5. The library preparation instrument 4 performs experiments such as pipetting, amplification, purification, hybridization, and washing on the required reagents and initial sample. Finally, the prepared sample library is injected into the empty consumable cartridge 6.

[0110] Step 2 (Consumables Loading and Unloading): For the consumable box 6 that is no longer used in the library preparation steps, it is output to the library preparation instrument 4 via the transfer module 41. The robotic arm 2 picks up the consumable box 6 and places it on the calibration device 8 for position calibration. Then, the robotic arm 2 puts the calibrated consumable box 6 into the storage device 5. For the reagent kit 6, consumable box 6 and sample clamping mechanism 33 required in the library preparation steps, the robotic arm 2 picks up the materials from the storage device 5 and places them on the calibration device 8 for position calibration. Then, the robotic arm 2 puts the calibrated materials into the transfer module 41 and inputs them into the library preparation instrument 4 via the transfer module 41.

[0111] Step 3 (Library Quality Control): The library preparation instrument 4 prepares the concentration detection system in an empty consumable box 6 by adding the prepared library to the concentration detection system and mixing the concentration detection system by the shaking module. Then, the consumable box 6 containing the concentration detection system is output from the library preparation instrument 4 through the transfer module 41. The robotic arm 2 picks up the consumable box 6 containing the concentration detection system and places it on the calibration device 8 for position calibration. Then, the robotic arm 2 places the calibrated consumable box 6 on the fluorescence quantitative detection device 7. The fluorescence quantitative detection device 7 performs specific target molecule concentration detection on the concentration detection system in the consumable box 6.

[0112] Step 4 (Library Mixing and DNB (DNA Nanoballs, i.e., the sequencing product)): Libraries of known concentrations are mixed in the library preparation instrument 4 by pipetting, thus preparing a mixed product. The library preparation instrument 4 performs pipetting, amplification, and other experiments on the mixed product, ultimately producing the sequencing product.

[0113] The consumable cartridge 6 containing the library and the product to be sequenced can be gripped on the transfer module 41 by the gripper 22 of the robotic arm 2 and placed into the calibration device 8. After the position is calibrated, the consumable cartridge 6 is gripped by the robotic arm 2 and placed into the sealing device 9 for sealing. The robotic arm 2 then puts the sealed consumable cartridge 6 back into the storage device 5.

[0114] The sequencing product produced by the library preparation instrument 4 is injected into the sequencing chip 32, and the sampled sequencing chip 32 is inserted into the gene sequencer 31 for sequencing of gene fragments.

[0115] like Figure 3 As shown, this fully automated NGS library preparation, hybridization, sequencing, and interpretation machine can be used for sample loading and sequencing of the sequencing chip 32. The sequencing device 3 includes a gene sequencer 31, a sample loading clamping mechanism 33, and a flipping mechanism 34. The gene sequencer 31 has a slot 311 for inserting the sequencing chip 32. The library preparation instrument 4 adds the library sample into the sequencing chip 32. The sample loading clamping mechanism 33 includes a clamping fixture 330 and a clamping base 336. The clamping base 336 has a second positioning groove 3361. The clamping fixture 330 is placed in the second positioning groove 3361. The clamping fixture 330 has an open state and a closed state. When the clamping fixture 330 is in contact with the gene chip 32, the clamping device 330 can be opened or closed. When the sequencer 31 comes into contact, it triggers the clamping fixture 330 to switch from the closed state to the open state. When the clamping fixture 330 is in the closed state, it clamps the sequencing chip 32. The flipping mechanism 34 can flip the clamping fixture 330 to drive the sequencing chip 32 after sample loading to flip. The robotic arm 2 also includes an execution end 21. The clamping member 22 is located on the execution end 21. The clamping member 22 can clamp the clamping fixture 330 or the clamping base 336. The execution end 21 can move between the library preparation instrument 4, the flipping mechanism 34 and the gene sequencer 31 so that the sequencing chip 32 is loaded, flipped and inserted into the preset position of the slot 311. First, robotic arm 2 grasps the sample loading clamping mechanism 33 and moves it into the library preparation instrument 4 for sample loading. Then, robotic arm 2 grasps the clamping base 336 and moves it to the flipping mechanism 34. The clamping base 336 moves the loaded sequencing chip 32, along with the clamping fixture 330, to the flipping mechanism 34. The flipping mechanism 34 grasps the clamping fixture 330, separating it from the clamping base 336. The flipping mechanism 34 then flips the clamping fixture 330, causing the loaded sequencing chip 32 to flip along with it. Robotic arm 2 then moves the flipped clamping fixture 330 and sequencing chip 32 to the gene sequencer 31. Through the movement of the actuator 21 of robotic arm 2, the sequencing chip 32 is inserted into the slot 331. The clamping fixture 330 clamps the sequencing chip 32 tightly, and the flipping mechanism 34 flips the clamping fixture 330 together with the sequencing chip 32 to prevent the sequencing chip 32 from falling out during the flipping process. The sequencing device 3, the robotic arm 2, and the library preparation instrument 4 work together to achieve automatic insertion of the sequencing chip 32, eliminating the need for specially arranged testing personnel to perform manual sequencing and eliminating the need for personnel scheduling, thus improving sequencing efficiency.

[0116] The sequencing chip 32 is roughly cuboid in shape. In this embodiment, the length direction of the sequencing chip 32 is defined as the X direction, the width direction as the Y direction, and the thickness direction as the Z direction. Any two of the X, Y, and Z directions are perpendicular to each other.

[0117] After the sequencing chip 32 is pre-inserted into the slot 311, the robotic arm 2 will cause the clamping fixture 330 to separate from the sequencing chip 32. Subsequently, the robotic arm 2 will move the clamping fixture 330, which is separated from the sequencing chip 32, along the X direction. That is, the actuator 21 has at least one degree of freedom to move along the X direction. The clamping fixture 330 will push the end of the sequencing chip 32 outside the slot 311 along the X direction, so that the sequencing chip 32 is inserted into the preset position of the slot 311. However, during the process of inserting the sequencing chip 32 into the slot 311, the gene sequencer 31 will apply pressure along the Z direction to the surface of the sequencing chip 32 so that the sequencing chip 32 can move along the Z direction before reaching the preset position. Sometimes, if the clamping fixture 330 is used to push the sequencing chip 32 along the X direction, the sequencing chip 32 may not be able to move along the Z direction at a certain position in the slot 311, causing a jamming phenomenon.

[0118] To address the issue of jamming during sequencing chip insertion, the robotic arm 2 also includes a chip pushing mechanism 23. This mechanism 23 is located at the actuator 21 and can push the jammed sequencing chip 32 to a preset position in the slot 311. When the sequencing chip 32 is jammed, the actuator 21 moves the chip pushing mechanism 23 to push the sequencing chip 32 to the preset position in the slot 311.

[0119] Specifically, such as Figure 3 As shown, the clamping member 22 and the chip pushing mechanism 23 are both connected to the execution end 21. When the sequencing chip 32 is stuck, the execution end 21 rotates so that the chip pushing mechanism 23 is facing the slot 311. The robot arm 2 drives the chip pushing mechanism 23 to insert the stuck sequencing chip 32 into the preset position of the slot 311.

[0120] The actuator 21 has at least one degree of freedom to rotate along an axis extending about the Z direction, so that the clamping member 22 and the chip pushing mechanism 23 are aligned with the slot 311 by rotation, so that the sequencing chip 32 clamped on the clamping fixture 330 is pre-inserted into the slot 311, or the stuck sequencing chip 32 is inserted into a preset position in the slot 311 by using the chip pushing mechanism 23.

[0121] like Figures 4-16As shown, the sample loading clamping mechanism 33 is used to guide and hold the sequencing chip 32 during sample loading. The sample loading clamping mechanism 33 is stored in a room-temperature storage unit 51. The sample loading clamping mechanism 33 also includes a sample loading seat 337 and a clamping fixture 330 for holding the sequencing chip 32. Both the sample loading seat 337 and the clamping fixture 330 are mounted on a clamping base 336. When the library preparation instrument 4 loads samples onto the sequencing chip 32 clamped on the clamping fixture 330, the sample loading seat 337 serves as a guide for sample loading.

[0122] The clamping base 336 is provided with a second positioning groove 3361, and the clamping fixture 330 is placed in the second positioning groove 3361. The clamping fixture 330 is used to clamp the sequencing chip 32. The clamping fixture 330 is placed in the second positioning groove 3361 of the clamping base 336, and the clamping base 336 positions the clamping fixture 330, thereby ensuring the positional accuracy of the sequencing chip 32. The clamping base 336 can also serve as a positioning reference when adding samples to the library preparation instrument 4 to ensure the accuracy of sample addition.

[0123] The sample loading holder 337 is simultaneously placed on the clamping base 336. A side positioning protrusion 323 is provided on the side wall of the end of the sequencing chip 32 that extends out of the clamping fixture 330. An open slot 3371 is provided on the sample loading holder 337 to engage with the side positioning protrusion 323. The interplay between the open slot 3371 and the side positioning protrusion 323 ensures the positional accuracy between the sample loading holder 337 and the sequencing chip 32.

[0124] The sequencing chip 32 is provided with a sample application port 322, a sample application holder 337 is located on one side of the sample application port 322, and a clamping fixture 330 is located on the opposite side of the sample application holder 337.

[0125] After the position of the sample loading port 337 is determined, the library preparation instrument 4 adds samples to the sequencing chip 32 through the sample loading port 337. After the sample addition is completed, the sample loading port 337 is removed from the library preparation instrument 4, and the clamping fixture 330 clamps the sequencing chip 32 for testing.

[0126] Preferably, the side positioning protrusion 323 is a cylinder disposed on the side of the sequencing chip 32.

[0127] The aforementioned opening slot 3371 is a square slot with an opening facing downwards, located on the lower surface of the sample feeding base 337.

[0128] Preferably, the sample loading stand 337 has a sample loading channel 3372 communicating with the sample loading port 322, and the diameter of the sample loading channel 3372 gradually decreases from the upper opening downwards. When the library preparation instrument 4 loads samples onto the sequencing chip 32 held on the clamping fixture 330, the samples first enter the sample loading channel 3372 and then enter the sample loading port 322. The diameter of the sample loading channel 3372 gradually decreases from the upper opening downwards, making it more convenient to load samples onto the sample loading port 322 of the sequencing chip 32 through the sample loading channel 3372 on the sample loading stand 337.

[0129] Preferably, the clamping base 336 has a weight reduction hole 3365 to reduce the weight of the clamping base 336.

[0130] Preferably, the clamping base 336 has a first observation window 3362, which is positioned opposite to the side positioning protrusion 323. The first observation window 3362 is used to observe whether the fit between the opening slot 3371 of the sample loading seat 337 and the side positioning protrusion 323 is in place.

[0131] In this embodiment, the sequencing chip 32 is provided with two sample loading ports 322, both of which can be used for sample loading.

[0132] Preferably, the clamping base 336 has a second observation window 3363, which is directly opposite the sample loading and detection area of ​​the sequencing chip 32. The second observation window 3363 is used to observe whether the sample in the sample loading and detection area of ​​the sequencing chip 32 flows from one sample loading port 322 to the other sample loading port 322.

[0133] Preferably, the clamping base 336 is provided with positioning walls 3364 on both sides, the positioning walls 3364 are located in front of the second positioning groove 3361, and the sample feeding seat 337 is provided with an open positioning slot 3373, which is engaged with the positioning wall 3364.

[0134] Preferably, the clamping fixture 330 has an open state and a closed state. When the clamping fixture 330 comes into contact with the gene sequencer 31, it triggers the clamping fixture 330 to switch from the closed state to the open state. When the clamping fixture 330 is in the closed state, it clamps the sequencing chip 32. The clamping fixture 330 includes a fixing component 331, a top cover 332, a reset component 333, and a locking component 334. The fixing component 331 includes a fixing member 3311 and a locking positioning member 3312. The fixing member 3311 has a mounting groove 3310, and the locking positioning member 3312 is disposed in the mounting groove 3310. The locking positioning member 3312 has a positioning part 33121 for positioning the sequencing chip 32.

[0135] One end of the top cover 332 is rotatably connected to the fixing member 3311, which is used to switch between opening or closing the mounting slot 3310.

[0136] The reset component 333 is disposed between the upper cover 332 and the fixing component 331. The reset component 333 is used to drive the upper cover 332 to open automatically after the locking component 334 unlocks the upper cover 332.

[0137] The locking component 334 is disposed between the upper cover 332 and the fixing component 331, and the locking component 334 is used to lock the closed upper cover 332.

[0138] When the top cover 332 is closed, the sequencing chip 32 is held between the fixing component 331 and the top cover 332.

[0139] In this embodiment, the upper cover 332 is rotatable relative to the fixing component 331, and can be opened or closed. The reset component 333 is used to automatically open the unlocked upper cover 332. When the upper cover 332 is closed, the locking component 334 locks the upper cover 332. When the upper cover 332 is locked, the sequencing chip 32 is clamped between the locking positioning member 3312 and the upper cover 332. When the clamping fixture 330 and the sequencing chip 32 are transferred or flipped, it is ensured that the sequencing chip 32 will not fall off.

[0140] During the process of inserting the sequencing chip 32 into the slot 311 of the gene sequencer 31, the locking component 334 is triggered to unlock due to the contact between the locking component 334 and the outer shell near the slot 311 of the gene sequencer 31. Subsequently, the top cover 332 automatically opens under the action of the reset component 333.

[0141] In this embodiment, the sequencing chip 32 can only be inserted into the slot 311 of the gene sequencer 31 for detection after the clamping fixture 330 and the sequencing chip 32 are simultaneously flipped 180° by the flipping mechanism 34, ensuring that the position of the sequencing chip 32 does not change during the flipping process.

[0142] Preferably, the upper cover 332 and the fixing member 3311 are rotatably connected by a rotating shaft 338. Connecting the upper cover 332 and the fixing member 3311 by the rotating shaft 338 has a simple structure and is easy to process.

[0143] Preferably, the reset assembly 333 includes a torsion spring mounting member 3331 and a torsion spring 3332, wherein the torsion spring mounting member 3331 is rotatably mounted on the rotating shaft 338 and connected to the upper cover 332. The torsion spring 3332 is sleeved on the rotating shaft 338 and mounted on the torsion spring mounting member 3331.

[0144] The torsion spring 3332 drives the torsion spring mounting part 3331 and the upper cover 332 to rotate synchronously along the rotating shaft 338, so that the upper cover 332 opens automatically. This structure is simple, easy to install, and low in cost.

[0145] Preferably, the locking assembly 334 includes a first locking connector 3341 and a second locking connector 3342. The first locking connector 3341 is disposed on the upper cover 332, and the second locking connector 3342 is disposed on the fixing assembly 331. The first locking connector 3341 and the second locking connector 3342 can be engaged or disengaged. When the second locking connector 3342 contacts the gene detector 31, the first locking connector 3341 and the second locking connector 3342 open, thereby triggering the locking assembly 334 to unlock. The first locking connector 3341 and the second locking connector 3342 engage to lock the upper cover 332. Specifically, the second locking connector 3342 is disposed on the fixing member 3311.

[0146] Preferably, the first locking connector 3341 includes a hook 33411, one end of which is connected to the upper cover 332. The second locking connector 3342 includes a hook connector 33421. A sliding groove 33112 is provided on the fixing member 3311 below the locking positioning member 3312. A slot 33421 is provided on the hook connector 33421. The first end of the hook connector 33421 is located outside the sliding groove 33112, and the second end of the hook connector 33421 extends into the sliding groove 33112 and can be engaged in the slot 334211. When the second locking connector 3342 contacts the gene detector 31, the second end of the hook connector 33421 slides in the sliding groove 33112, causing the other end of the hook 33411 to disengage from the slot 334211, thereby triggering the locking assembly 334 to unlock.

[0147] The locking assembly 334 also includes a locking spring 3343, which is used to keep the hook 33411 and the slot 334211 in a locked state and is connected to one end of the locking spring 3343. The other end of the locking spring 3343 is connected to the groove wall of the sliding groove 33112.

[0148] Specifically, there are two hooks 33411, which are located on both sides of the sequencing chip 32 along the Y direction. The hooks 33411 and the hook connectors 33421 are set in a one-to-one correspondence.

[0149] Specifically, the hook 33411 has a hook groove, which can engage with the side wall of the slot 334211 (e.g., Figure 7 On the left side wall of the slot). When the locking assembly 334 locks the closed upper cover 332, the locking spring 3343 is in a compressed state. The locking spring 3343 causes the side wall of the slot 334211 to be locked in the hook slot, so that the hook 33411 and the slot 334211 are in a locked state.

[0150] Specifically, the slot 334211 is an elongated slot that extends along the length of the sliding slot 33112. The hook 33411 can move within the elongated slot, allowing the hook 33411 to switch between engaging and disengaging states with the slot 334211. The elongated slot also facilitates the hook 33411 completely disengaging from the slot 334211 after disengaging from it, as it moves upward with the top cover 332.

[0151] During the process of inserting the sequencing chip 32 into the slot 311 of the gene sequencer 31, the sequencing chip 32 is first partially inserted into the slot 311. The first end of the hook connector 33421 abuts against the outer shell near the slot 311 of the gene sequencer 31 before the fixing member 3311. The fixing member 3311 continues to approach the slot 311, and the first end of the hook connector 33421 is squeezed, thereby causing the hook connector 33421 to slide into the sliding groove 33112. The left side wall of the groove 334211 retracts, thereby causing the hook 33411 to disengage from the groove 334211. The upper cover 332 is no longer subject to the locking force of the locking component 334. The upper cover 332 automatically opens under the action of the reset component 333, that is, the second locking connector 3342 contacts the outer shell near the slot 311 of the gene sequencer 31, triggering the locking component 334 to unlock. When the latch connector 33421 is no longer compressed by the gene sequencer 31, the locking spring 3343 will cause the latch connector 33421 to reset. However, since the top cover 332 opening will cause the latch 33411 to move, the latch 33411 will completely separate from the latch connector 33421. Before the locking spring 3343 causes the latch connector 33421 to reset, the locking spring 3343 is in a compressed state. Therefore, there is a risk that the latch connector 33421 will dislodge from the sliding groove 33112 when resetting. The hook connector 33421 has a limiting hole 334213, which is not connected to the slot 334211. The locking assembly 334 also includes a limiting member 3345, which is installed in the fixing member 3311 and inserted into the limiting hole 334213. The limiting hole 334213 is an elongated hole that extends along the length of the sliding groove 33112, allowing the limiting member 3345 to have freedom within the limiting hole 334213. This allows the hook connector 33421 to slide freely within the sliding groove 33112 without being affected, i.e., it does not affect the triggering of the locking assembly 334 to unlock. However, the limiting member 3345 can also prevent the hook connector 33421 from coming out of the sliding groove 33112.

[0152] Preferably, the fixing member 3311 has a locking cavity 33113, and the locking positioning member 3312 has a locking relief groove 33124. The locking relief groove 33124 and the locking cavity 33113 are arranged vertically opposite each other. When the hook connector 33421 is in a free and unpressed state, the slot 334211 is connected to the locking cavity 33113, and the hook 33411 passes through the locking relief groove 33124 and the locking cavity 33113 and is engaged in the slot 334211.

[0153] Preferably, the contact surface where the hook 33411 engages with the slot 334211 is the second arc surface 334212, which is curved from the bottom to the top surface to facilitate engagement. When the hook connector 33421 slides into the sliding groove 33112, the hook 33411 will disengage from the second arc surface 334212 to unlock. When it is necessary to engage the hook 33411 with the second arc surface 334212, the second locking connector 3342 can be manually pressed to insert the hook 33411 into the slot 334211. After the pressing force is removed, the hook 33411 will engage with the slot 334211.

[0154] Preferably, the locking and positioning member 3312 is provided with two sets of positioning parts 33121, which are spaced apart along the axial direction of the rotating shaft 338. The sequencing chip 32 is placed between the two sets of positioning parts 33121, and the sides of the sequencing chip 32 abut against the two sets of positioning parts 33121 respectively. The sides of the sequencing chip 32 are positioned by the positioning parts 33121 on both sides.

[0155] Preferably, the edge of the top surface of the positioning part 33121 near the sequencing chip 32 is a first arc surface 33122. When the sequencing chip 32 is placed between the two positioning parts 33121, the first arc surface 33122 is provided to prevent scratching the sequencing chip 32. In addition, it also facilitates the sequencing chip 32 to enter between the two positioning parts 33121.

[0156] Preferably, the locking positioning member 3312 is further provided with a first positioning groove 33123, and the sequencing chip 32 is provided with an end positioning protrusion 321 that cooperates with the first positioning groove 33123.

[0157] In this embodiment, the first positioning groove 33123 and the end positioning protrusion 321 provided on the sequencing chip 32 cooperate to further ensure the positional accuracy of the sequencing chip 32 on the locking positioning member 3312.

[0158] In this embodiment, the first positioning groove 33123 is located at one end of the positioning part 33121 near the rotating shaft 338, and the length of the first positioning groove 33123 is set along the axial direction of the rotating shaft 338.

[0159] The end positioning protrusion 321 is inserted into the first positioning groove 33123 for positioning. When the upper cover 332 is closed, the sequencing chip 32 is clamped between the upper cover 332 and the locking positioning member 3312. Through the cooperation between the end positioning protrusion 321 and the first positioning groove 33123, the position of the sequencing chip 32 will not change during the movement.

[0160] Preferably, the fixing member 3311 is provided with a clamping hole 33111, and the gripper of the flipping mechanism 34 or the clamping member 22 of the robot arm 2 clamps the fixing member 3311 through the clamping hole 33111.

[0161] When it is necessary to flip the sequencing chip 32, the grippers of the flipping mechanism 34 (the first gripping and positioning member of the first gripper 343 and the second gripping and positioning member 3441 of the second gripper 344) are engaged with the clamping holes 33111 on the fixing member 3311. The grippers of the flipping mechanism 34 flip the clamping fixture 330 and the sequencing chip 32. When it is necessary to move the flipped sequencing chip 32 to be pre-inserted into the slot 311 of the gene sequencer 31, the gripper 22 of the robot arm 2 is engaged with the clamping holes 33111 of the fixing member 3311. The movement of the robot arm 2 moves the fixing member 3311 and the sequencing chip 32 to the vicinity of the slot 311 and pre-inserts the sequencing chip 32 into the slot 311.

[0162] Preferably, the clamping holes 33111 are disposed on the side wall of the positioning part 33121, and the clamping holes 33111 are symmetrically disposed on both sides of the sequencing chip 32.

[0163] During the process of inserting the sequencing chip 32 into the slot 311 of the gene sequencer 31, the sequencing chip 32 is first partially inserted into the slot 311. The first end of the hook connector 33421 abuts against the outer shell near the slot 311 of the gene sequencer 31 before the fixing member 3311. The fixing member 3311 continues to approach the slot 311, and the first end of the hook connector 33421 is squeezed, thereby causing the hook connector 33421 to slide into the sliding groove 33112. The left side wall of the groove 334211 retracts, thereby causing the hook 33411 to disengage from the groove 334211. The upper cover 332 is no longer subject to the locking force of the locking component 334. The upper cover 332 automatically opens under the action of the reset component 333, that is, the second locking connector 3342 contacts the outer shell near the slot 311 of the gene sequencer 31, triggering the locking component 334 to unlock. When the hook connector 33421 is no longer squeezed by the gene sequencer 31, the locking spring 3343 will drive the hook connector 33421 to reset. However, since the top cover 332 opens, it will drive the hook 33411 to move, thus making the hook 33411 completely separate from the hook connector 33421.

[0164] After the top cover 332 opens automatically, the sequencing chip 32 is pre-inserted into the slot 311. The robotic arm 2 can move the fixing component 331 downwards, separating the sequencing chip 32 from the fixing component 331. Then, the robotic arm 2 drives the fixing component 3311 to push the sequencing chip 32 to the preset position. If the sequencing chip 32 is inserted into the preset position, the gene sequencer 31 will display that the insertion is successful. If the sequencing chip 32 is stuck during insertion, causing it to not be inserted into the preset position, the gene sequencer 31 will not detect the sequencing chip 32. In this case, the robotic arm 2 needs to drive the chip pushing mechanism 23 to push the sequencing chip 32 to insert it into the preset position of the slot 311.

[0165] like Figure 17 As shown, the gene sequencer 31 has at least one slot 311 for the sequencing chip 32 to be inserted into the gene sequencer 31.

[0166] It should be noted that the structure and working principle of the gene sequencer 31 and the sequencing chip 32 are existing technologies and will not be described in detail in this utility model.

[0167] like Figure 17 As shown, when the sequencing chip 32 is aligned with the slot 311 in the Y and Z directions, one end of the sequencing chip 32 in the X direction (e.g.) Figure 17 The left end of the sequencing chip 32 is pre-inserted into the slot 311, and the robotic arm 2 drives the chip pushing mechanism 23 to push the sequencing chip 32 in the X direction to the other end (e.g., in the X direction) of the slot 311. Figure 17 The sequencing chip 32 is moved along the X direction to the right end of the slot 311 so that it can be inserted into the preset position of the slot 311. During the insertion of the sequencing chip 32 into the slot 311, if the sequencing chip 32 gets stuck in the Z direction, the robotic arm 2 drives the chip pushing mechanism 23 to move the sequencing chip 32 in the Z direction so that it moves into place in the Z direction.

[0168] Specifically, the actuator 21 of the robotic arm 2 is connected to the chip pushing mechanism 23, and the actuator 21 can move to any position in three-dimensional space, and has at least translational degrees of freedom in the X and Z directions, so that the chip pushing mechanism 23 can translate at least in the X and Z directions. The robotic arm 2 drives the chip pushing mechanism 23 to translate in the X direction, which can pre-insert the sequencing chip 32 into the slot 311 in the X direction. By translating the chip pushing mechanism 23 in the Z direction, the sequencing chip 32 can be moved in the Z direction, so as to move the sequencing chip 32 into place when it is stuck.

[0169] like Figure 18 and Figure 19As shown, the chip pushing mechanism 23 includes a pushing base 231, a Z-axis adjustment component 232, and a pushing member 233. The pushing base 231 is fixedly mounted on the execution end 21 of the robot arm 2 so that the robot arm 2 can drive the chip pushing mechanism 23 to move. The Z-axis adjustment component 232 is disposed on one side of the pushing base 231, for example, on the side of the pushing base 231 away from the execution end 21 of the robot arm 2. The Z-axis adjustment component 232 includes a Z-axis moving member 2321 that is movable in the Z direction. The pushing member 233 is fixedly mounted on the Z-axis moving member 2321, and the pushing member 233 is located on the side of the Z-axis moving member 2321 away from the pushing base 231. Thus, the pushing member 233 has a translational degree of freedom in the Z direction by means of the Z-axis moving member 2321, that is, the pushing member 233 can move in the Z direction relative to the pushing base 231.

[0170] like Figure 18 As shown, the pushing component 233 includes a pushing surface 2331, a first actuating block 2332, and a second actuating block 2333. The pushing surface 2331 is located on the side of the pushing component 233 away from the Z-direction moving component 2321, and the pushing surface 2331 is perpendicular to the X-direction. The pushing surface 2331 is used to push the sequencing chip 32 in the X-direction. The first actuating block 2332 is located at one end of the pushing surface 2331 in the Z-direction (e.g., ...). Figure 18 The second actuating block 2333 extends along the X direction from the upper end of the push surface 2331 and away from the Z-direction moving member 2321. The second actuating block 2333 extends from the other end of the push surface 2331 in the Z direction (e.g., from the other end of the push surface 2331 in the Z direction). Figure 18 It extends along the X direction and away from the Z-direction moving part 2321 at the lower end of the middle pushing surface 2331.

[0171] Thus, a pushing groove is formed on the side of the pushing component 233 away from the Z-direction moving component 2321, the pushing surface 2331 serves as the bottom surface of the pushing groove, and the first actuating block 2332 and the second actuating block 2333 serve as the sidewalls of the pushing groove.

[0172] When the robotic arm 2 drives the push base 231 to move in the X direction, the push surface 2331 can push the sequencing chip 32 to move in the X direction. When the robotic arm 2 drives the push base 231 to move in the Z direction, the first actuating block 2332 or the second actuating block 2333 can actuate the sequencing chip 32 in the Z direction so that the sequencing chip 32 moves into place after being inserted into the slot 311.

[0173] like Figure 18 As shown, the first actuating block 2332 has a first actuating surface 23321 on the side near the pushing surface 2331. The first actuating surface 23321 serves as one of the walls of the pushing groove and is used to contact the sequencing chip 32 when the sequencing chip 32 is actuated. The first actuating surface 23321 is a horizontal plane or an inclined surface formed by rotating the horizontal plane along the Y-axis.

[0174] like Figure 18 As shown, the second actuating block 2333 has a second actuating surface 23331 on the side near the pushing surface 2331. The second actuating surface 23331 serves as one of the walls of the pushing groove and is used to contact the sequencing chip 32 when the sequencing chip 32 is actuated. The second actuating surface 23331 is a horizontal plane or an inclined surface formed by rotating the horizontal plane along the Y-axis.

[0175] like Figure 18 and Figure 19 As shown, the Z-axis adjustment assembly 232 also includes a Z-axis mounting base 2322, a Z-axis guide member 2323, and a Z-axis elastic member 2324. The Z-axis mounting base 2322 is disposed on the push base 231 so that the actuator 21 of the robot arm 2 can drive the Z-axis adjustment assembly 232 to move. The Z-axis guide member 2323 is fixedly disposed on the Z-axis mounting base 2322 and extends along the Z direction. For example, the Z-axis guide member 2323 may be a guide rod. The Z-axis moving member 2321 mentioned above is movably disposed on the Z-axis guide member 2323, so that the Z-axis moving member 2321 can translate along the Z direction. Z-axis elastic members 2324 are disposed on both sides of the Z-axis moving member 2321 along the Z direction. For example, the Z-axis elastic member 2324 may be a spring, which may be sleeved on the Z-axis guide member 2323.

[0176] By providing Z-axis elastic members 2324 on both sides of the Z-axis moving member 2321, the Z-axis moving member 2321 can be held in a preset position, such as the central position of the Z-axis guide member 2323, when no external force is applied. When the Z-axis moving member 2321 is moved by an external force, the Z-axis guide member 2323 moves accordingly, thereby extending or compressing, so that when the external force is removed, the Z-axis guide member 2323 drives the Z-axis moving member 2321 to reset.

[0177] like Figure 19 As shown, the Z-axis mounting base 2322 includes a first mounting block 23221 and a second mounting block 23222. The second mounting block 23222 is located on one side of the first mounting block 23221 along the Z direction, for example, the second mounting block 23222 is located below the first mounting block 23221. One end of the Z-axis guide member 2323 ( Figure 19 The upper middle end) is fixedly installed on the first mounting block 23221, and the other end of the Z-direction guide component 2323 ( Figure 19 The lower part is fixedly disposed on the second mounting block 23222. In addition, Z-axis elastic members 2324 are provided between the first mounting block 23221 and the Z-axis moving member 2321, and between the second mounting block 23222 and the Z-axis moving member 2321.

[0178] like Figure 18 and Figure 19 As shown, the chip pushing mechanism 23 also includes an X-direction buffer component 234, which is disposed between the pushing base 231 and the Z-direction mounting base 2322, so that the Z-direction mounting base 2322 and the pushing base 231 can move relative to each other in the X direction, thereby providing a buffer in the X direction for the pushing component 233 when pushing the sequencing chip 32 to move in the X direction.

[0179] like Figure 18 and Figure 19 As shown, the X-direction buffer assembly 234 includes an X-direction moving member 2341, an X-direction guiding member 2342, and an X-direction elastic member 2343. The X-direction moving member 2341 is disposed between the Z-direction mounting base 2322 and the push base 231, and the Z-direction mounting base 2322 is fixedly disposed on the X-direction moving member 2341. The X-direction guiding member 2342 (e.g., a guide rod) extends in the X direction, one end of the X-direction guiding member 2342 is fixedly disposed on the X-direction moving member 2341, and the other end of the X-direction guiding member 2342 is movably disposed on the push base 231. The X-direction elastic member 2343 (e.g., a spring) is disposed between the X-direction moving member 2341 and the push base 231.

[0180] By providing an X-axis elastic member 2343, a preset distance is maintained between the X-axis moving member 2341 and the pushing base 231. When the pushing member 233 is subjected to an external force, the distance between the X-axis moving member 2341 and the pushing base 231 decreases, and the X-axis elastic member 2343 is compressed. When the external force is removed, the X-axis elastic member 2343 extends, restoring the distance between the X-axis moving member 2341 and the pushing base 231 to its original position.

[0181] like Figure 19 As shown, in one embodiment, the push base 231 is provided with an X-direction channel 2311 that runs through the X direction, one end of the X-direction guide member 2342 near the push base 231 is movably disposed in the X-direction channel 2311, and one end of the X-direction guide member 2342 near the X-direction moving member 2341 is fixedly disposed in the X-direction moving member 2341.

[0182] like Figure 19 and Figure 20 As shown, in one embodiment, the X-direction guide member 2342 is a shoulder screw, one end of which is threaded to the X-direction moving member 2341, and the other end of which is movably disposed in the X-direction channel 2311.

[0183] The sequencing chip 32 is first pre-inserted into the slot 311. Then, the actuator 21 of the robotic arm 2 drives the chip pushing mechanism 23 to move, so that the pushing component 233 is aligned with the sequencing chip 32 in the Y and Z directions. Then, the actuator 21 of the robotic arm 2 drives the chip pushing mechanism 23 to move along the X direction, so that the pushing component 233 contacts one end of the sequencing chip 32 and pushes the sequencing chip 32 to move along the X direction. If the sequencing chip 32 gets stuck in the Z direction during the movement, the actuator 21 of the robotic arm 2 drives the chip pushing mechanism 23 to move in the Z direction, so that the first actuating block 2332 or the second actuating block 2333 contacts the sequencing chip 32 and actuates the sequencing chip 32 in the Z direction, so that the sequencing chip 32 moves into place in the Z direction.

[0184] Figure 21 and Figure 22 A flipping mechanism 34 according to a first embodiment of the present invention is shown.

[0185] The flipping mechanism 34 includes a flipping fixed base 341, a flipping drive assembly 342, a first gripper 343, and a second gripper 344.

[0186] A flip-mounted retainer 341 is mounted on the frame 1. For example, in... Figure 21 and Figure 22 In this design, the flip-mounted base 341 is a vertical plate, the lower end of which is used to connect to the frame 1. Specifically, the lower end of the vertical plate may have mounting holes 3411 for connection to the frame 1 via fasteners (e.g., screws).

[0187] The flip drive assembly 342 can be disposed on the flip fixing base 341, that is, the flip fixing base 341 provides support for the flip drive assembly 342. For example, in... Figure 21 and Figure 22 In this case, the flip drive assembly 342 can be disposed on the upper end of the flip fixing base 341.

[0188] The flip drive assembly 342 is connected to the first gripper 343 and the second gripper 344, and can drive the first gripper 343 and the second gripper 344 to move and / or rotate synchronously.

[0189] Specifically, the flip drive assembly 342 includes a rotation drive component 3421 and a clamping drive component 3422 connected to each other.

[0190] The rotary drive component 3421 is configured to drive the clamping drive component 3422 to rotate about the rotation axis 3423. The rotation axis 3423 may be the axis of the rotary drive component 3421 itself. Figure 21 and Figure 22 In this configuration, the axis of the rotation drive component 3421 and the axis of the clamping drive component 3422 are collinear. Furthermore, by way of example, in... Figure 21 and Figure 22 In the middle, the rotation axis 3423 extends in the horizontal direction.

[0191] The clamping drive component 3422 has a first actuating end 34221 and a second actuating end 34222. A first gripper 343 is disposed at the first actuating end 34221, and a second gripper 344 is disposed at the second actuating end 34222. At least one of the first actuating end 34221 and the second actuating end 34222 is configured to be movable between a clamping position and a releasing position, so that the first gripper 343 and the second gripper 344 can switch between the clamping state and the releasing state.

[0192] Specifically, in one example, the first actuator 34221 is fixed, and the second actuator 34222 is movable between a clamping position and a releasing position. Thus, the first gripper 343 cannot move, and the second gripper 344 can move under the drive of the second actuator 34222.

[0193] In another example, both the first actuator 34221 and the second actuator 34222 are movable between a clamping position and a releasing position, thereby both the first gripper 343 and the second gripper 344 are movable.

[0194] The distance between the first gripper 343 and the second gripper 344 is smaller in the clamped state than in the relaxed state, so that the clamping fixture 330 holding the sequencing chip 32 can be clamped between the first gripper 343 and the second gripper 344.

[0195] like Figure 21 As shown, a clamping area is formed between the first gripper 343 and the second gripper 344, and the clamping fixture 330 is located within the clamping area. Specifically, the first gripper 343 is located on one side of the clamping fixture 330, and the second gripper 344 is located on the other side of the clamping fixture 330, that is, the first gripper 343 and the second gripper 344 can clamp the clamping fixture 330 from opposite directions on opposite sides.

[0196] like Figure 21 As shown, a clamping hole 33111 is provided on one side wall of the fixing member 3311 of the clamping fixture 330, and a clamping hole 33111 is also provided on the other side wall of the clamping fixture 330. The clamping hole 33111 can be a through hole or a blind hole.

[0197] A first clamping positioning element (not shown) is provided on the side of the first gripper 343 facing the clamping area. The shape and size of the first clamping positioning element correspond to the shape and size of the clamping hole 33111. When the first gripper 343 is in the clamping state, the first clamping positioning element is inserted into the clamping hole 33111.

[0198] A second clamping positioning element 3441 is provided on the side of the second gripper 344 facing the clamping area (see...). Figure 23 The shape and size of the second clamping positioning member 3441 correspond to the shape and size of the clamping hole 33111. When the second gripper 344 is in the clamping state, the second clamping positioning member 3441 is inserted into the clamping hole 33111.

[0199] In summary, according to the first embodiment of the present invention, when the flipping mechanism 34 clamps the clamping fixture 330, the first clamping positioning member of the first jaw 343 is inserted into the clamping hole 33111 of the clamping fixture 330, and the second clamping positioning member 3441 of the second jaw 344 is inserted into the clamping hole 33111 on the other side of the clamping fixture 330. Thus, the positioning accuracy when clamping the clamping fixture 330 is improved through the mechanical cooperation between the clamping positioning member and the clamping hole 33111.

[0200] Optionally, the first clamping positioning member can be detachably disposed on the first gripper 343 to facilitate replacement of the worn first clamping positioning member, or replacement of the first clamping positioning member with a different shape and / or size. For similar reasons, the second clamping positioning member 3441 can also be detachably disposed on the second gripper 344.

[0201] Optionally, the first clamping and positioning element is selected from either a positioning protrusion or a positioning pin. The positioning protrusion can be a semi-circular protrusion. The positioning pin can be a cylindrical positioning pin or a conical positioning pin, and the shape of the clamping hole 33111 is adapted to the shape of the first clamping and positioning element. For example, when the positioning pin is a conical positioning pin, the clamping hole 33111 is a conical hole. For similar reasons, the second clamping and positioning element 3441 can be selected from either a positioning protrusion or a positioning pin.

[0202] Optionally, there can be multiple first clamping and positioning elements, such as two or three. By setting multiple first clamping and positioning elements, the positioning accuracy when clamping the clamping fixture 330 can be further improved. For similar reasons, there can also be multiple second clamping and positioning elements 3441.

[0203] exist Figure 21 and Figure 22 In this design, the rotary drive component 3421 and the clamping drive component 3422 are two independent components. Specifically, the rotary drive component 3421 can be a rotary cylinder or a rotary electric cylinder. The clamping drive component 3422 can be a finger cylinder or a finger electric cylinder. The rotary drive component 3421 and the clamping drive component 3422 can be connected to each other via a connecting assembly 345.

[0204] For example, the connecting assembly 345 includes a first flip connector 3451 and a second flip connector 3452. The first flip connector 3451 is connected to the rotating seat of the rotary flip drive assembly 342. The second flip connector 3452 is connected to the first flip connector 3451 and is also connected to the outer side wall of the clamping drive component 3422. Thus, when the rotating seat of the rotary drive component 3421 rotates, the first flip connector 3451 rotates accordingly, driving the second flip connector 3452 to rotate synchronously, and ultimately the clamping drive component 3422 also rotates synchronously.

[0205] like Figure 22 As shown, the flipping mechanism 34 also includes a sensor assembly 346, which is configured to measure the angle of rotation of the clamping drive component 3422. When the sensor assembly 346 detects that the clamping drive component 3422 has rotated by a preset angle (e.g., 45 degrees, 90 degrees, 180 degrees, etc.), the rotational movement of the rotation drive component 3421 is stopped.

[0206] For example, the sensor assembly 346 includes a slotted photoelectric switch 3461 and a switch baffle 3462. The slotted photoelectric switch 3461 is fixedly disposed relative to the rotary drive component 3421, for example, the slotted photoelectric switch 3461 is fixed to the flip-mounted fixing base 341 via a switch mounting base 3463. The switch baffle 3462 is disposed on the connecting assembly 345 (e.g., the first flip-mounted connecting member 3451). When the connecting assembly 345 rotates under the drive of the rotary drive component 3421, the switch baffle 3462 follows the rotation of the connecting assembly 345, and the movement trajectory of the switch baffle 3462 passes through the slotted photoelectric switch 3461. Thus, the slotted photoelectric switch 3461 can detect that the connecting assembly 345 has rotated by a preset angle, that is, the clamping drive component 3422 has rotated by a preset angle.

[0207] Figure 23 A perspective view of the flipping mechanism 34 according to a second embodiment of the present invention is shown. Unlike the first embodiment, the flipping drive assembly 342 is a rotating gripper, meaning that the rotating drive component 3421 and the clamping drive component 3422 are integrated into one assembly. The rotating gripper can either drive the first gripper 343 and the second gripper 344 to move, or drive the first gripper 343 and the second gripper 344 to rotate synchronously.

[0208] The specific process of sequencing the product to be sequenced is as follows:

[0209] Step 1: Manually assemble the sequencing chip 32 and the sample clamping mechanism 33 together and place them on the room temperature storage mechanism 51: The first positioning groove 33123 and the end positioning protrusion 321 cooperate, the positioning part 33121 positions the sequencing chip 32, so that the sequencing chip 32 is clamped on the clamping fixture 330, the hook 33411 is engaged in the slot 334211, the torsion spring 3332 and the locking spring 3343 are both in a compressed state, then place the sequencing chip 32 and the clamping fixture 330 in the mounting groove 3310, and make the sample dispensing port 322 face upward. Then, place the sample dispensing seat 337 on the clamping base 336, so that the positioning wall 3364 cooperates with the opening positioning slot 3373, and the side positioning protrusion 323 of the sequencing chip 32 cooperates with the opening slot 3371 of the sample dispensing seat 337.

[0210] Step 2: The gripper 22 of the robotic arm 2 places the sample clamping mechanism 33 holding the sequencing chip 32 on the calibration device 8 at the calibration position, and then transfers it to the transfer module 41 of the library preparation instrument 4:

[0211] Step 3: The transfer module 41 transfers the sample clamping mechanism 33 and the sequencing chip 32 to the library preparation instrument 4. The library preparation instrument 4 injects the sequencing product into the sample port 322 through the sample channel 3372. After the sample is added, the built-in gripper of the library preparation instrument 4 removes the sample holder 337. The sample holder 337 is collected and sorted by the experimenter after the experiment is received. The clamping fixture 330, the clamping base 336 and the sequencing chip 32 are output from the library preparation instrument 4 by the transfer module 41.

[0212] Step 4: The gripper 22 of the robotic arm 2 grips the gripping base 336 to transport the gripping fixture 330, the gripping base 336 and the sequencing chip 32 as a whole to the calibration position on the calibration device 8. Then, the robotic arm 2 transports the calibrated gripping fixture 330, the gripping base 336 and the sequencing chip 32 as a whole to the flipping mechanism 34.

[0213] Step 5: The flipping mechanism 34 grips the clamping fixture 330 with the sequencing chip 32, so that the clamping fixture 330 is separated from the clamping base 336. The clamping fixture 330 with the sequencing chip 32 is flipped 180° by the flipping mechanism 34, and the robot arm 2 takes away the clamping base 336. The clamping base 336 can be clamped onto the calibration device 8 or directly clamped onto the room temperature storage mechanism 51.

[0214] Step 6: The clamping member 22 is clamped onto the clamping hole 33111 of the flipped clamping fixture 330, and the flipped clamping fixture 330 is moved to the slot 311 of the gene sequencer 31. The actuator 21 drives the clamping member 22 and the clamping fixture 330 to move in the X direction to pre-insert the sequencing chip 32 into the slot 311. When the latch connector 33421 contacts the outer shell around the slot 311, it triggers the latch connector 33421 to unlock from the latch 33411. The upper cover 332 opens the mounting slot 3310 under the action of the reset component 333. The actuator 21 moves the clamping member 22 downward along the Y direction, causing the sequencing chip 32 to separate from the clamping fixture 330. Then, the actuator 21 moves the clamping member 22 along the X direction, causing the outer surface of the fixing member 3311 to contact the end of the sequencing chip 32 located outside the slot 311, thereby pushing the sequencing chip 32 to the preset position of the slot 311. When the sequencing chip 32 gets stuck, the actuator 21 rotates along the Z direction axis, causing the chip pushing mechanism 23 to face the slot 311, thereby inserting the stuck sequencing chip 32 into the preset position of the slot 311.

[0215] Step 7: The gripper 22 of the robotic arm 2 sends the gripper 330, which has been separated from the sequencing chip 32, back into the storage device 5.

[0216] like Figures 24-29 As shown, the low-temperature storage mechanism 52 includes an anti-condensation component 521, which includes a storage mounting base 5211, a pressure cap component 5212, and a spring-loaded component 5213. The storage mounting base 5211 has a mounting surface 521113, the pressure cap component 5212 is mounted on the mounting surface 521113, and the first end of the spring-loaded component 5213 is mounted on the mounting surface 521113. The pressure cap component 5212 has a first state and a second state. When the pressure cap component 5212 is in the first state, the second ends of both the pressure cap component 5212 and the spring-loaded component 5213 abut against the sealing surface of the consumable box 6. When the pressure cap component 5212 is in the second state, the pressure cap component 5212 is away from the sealing surface of the consumable box 6, and the second end of the spring-loaded component 5213 extends beyond the pressure cap component 5212 and abuts against the sealing surface of the consumable box 6. When the capping component 5212 is in the first state, it abuts against the sealing surface of the consumable box 6, preventing condensate from entering the consumable box 6 from the sealing surface. When the capping component 5212 is in the second state, it moves away from the sealing surface of the consumable box 6, but the second end of the spring-loaded component 5213 still abuts against the sealing surface of the consumable box 6, preventing the consumable box 6 from moving with the capping component 5212, thus preventing damage to the consumable box 6 and the refrigerated items on it, extending the service life of the consumable box 6, and ensuring the safety of low-temperature storage.

[0217] In this embodiment, the refrigerated item enters the consumable box 6 through the opening of the consumable box 6, and the plane where the opening of the consumable box 6 is located is the sealing surface of the consumable box 6.

[0218] Furthermore, the spring-loaded component 5213 includes a spring-loaded element 52131 and an elastic element 52133. One end of the elastic element 52133 is connected to the mounting surface 521113, and the other end of the elastic element 52133 is connected to the spring-loaded element 52131. When the pressure cap component 5212 is in the first state, the spring-loaded element 52131 abuts against the sealing surface of the consumable box 6. When the pressure cap component 5212 is in the second state, the spring-loaded element 52131 extends beyond the pressure cap component 5212 and abuts against the sealing surface of the consumable box 6. When the capping component 5212 is in the first state, the elastic element 52133 is in a compressed state; when the capping component 5212 is in the second state, the spring-loaded element 52131 still abuts against the sealing surface of the consumable box 6 under the elastic force of the elastic element 52133. At this time, the capping component 5212 has moved away from the sealing surface of the consumable box 6, preventing the consumable box 6 from moving with the capping component 5212, so as to prevent damage to the consumable box 6 and the refrigerated items on the consumable box 6, extend the service life of the consumable box 6, and ensure the safety of low-temperature storage.

[0219] Preferably, the elastic element 52133 can be a spring.

[0220] like Figure 24 and Figure 25 As shown, the anti-condensation component 521 also includes a fastener 5215, and the cover component 5212 includes a pressure plate 52121. The pressure plate 52121 is connected to the mounting surface 521113 by the fastener 5215. The pressure plate 52121 can be moved away from or pressed against the sealing surface of the consumable box 6.

[0221] Furthermore, the pressure cap component 5212 also includes an elastic pad 52122, which is disposed on the pressure plate 52121. The elastic pad 52122 can move away from or abut against the sealing surface of the consumable box 6. The elastic pad 52122 ensures a soft contact between the pressure plate 52121 and the sealing surface of the consumable box 6, preventing damage to the consumable box 6. The elastic pad 52122 also provides a better seal on the sealing surface of the consumable box 6, thus improving the anti-condensation effect. Simultaneously, the elastic pad 52122 provides some heat insulation, keeping the surface temperature of the consumable box 6 at a low level and preventing significant fluctuations, thereby ensuring the refrigeration effect of the low-temperature storage mechanism 52.

[0222] Furthermore, the anti-condensation assembly 521 also includes a buffer member 5214, one end of which is connected to the mounting surface 521113, and the other end is connected to the pressure plate 52121. The buffer member 5214 acts as a buffer between the pressure cap component 5212 and the consumable box 6, preventing damage to the consumable box 6.

[0223] Specifically, the buffer 5214 can be a spring.

[0224] Furthermore, the pressure cap component 5212 is provided with a relief groove, which is used to avoid the spring buffer component 52131.

[0225] Specifically, the pressure plate 52121 is provided with a first clearance groove 521211, and the elastic pad 52122 is provided with a second clearance groove 521221. The first clearance groove 521211 and the second clearance groove 521221 are arranged opposite each other to form a clearance groove together, so as to avoid interference with the movement of the buffer 52131.

[0226] Furthermore, the number of buffer components 5213 is multiple sets, and the multiple sets of buffer components 5213 are arranged at intervals along the mounting surface 521113, with the clearance groove corresponding to each buffer component 5213.

[0227] like Figures 26-29 As shown, the spring-damping component 5213 also includes a spring-damping fixing seat 52132, which is mounted on the mounting surface 521113. A guide cavity is formed between the spring-damping fixing seat 52132 and the mounting surface 521113. An elastic element 52133 is disposed within the guide cavity, and the spring-damping component 52131 is guided by the guide cavity. The guide cavity ensures the accuracy of the movement of the spring-damping component 52131 under the elastic force of the elastic element 52133.

[0228] Furthermore, the spring-loaded component 52131 includes a guide portion 521311 and a pressing portion 521312. The guide portion 521311 protrudes from the outer surface of the pressing portion 521312 and is movably disposed within the guide cavity. The pressing portion 521312 passes through the spring-loaded fixing seat 52132 and can extend beyond the pressure cap component 5212, and can abut against the sealing surface of the consumable box 6. This arrangement prevents the spring-loaded component 52131 from dislodging from the guide cavity.

[0229] Furthermore, a first guide groove 521112 is provided on the mounting surface 521113, and a second guide groove 521322 is provided on the spring-loaded fixing seat 52132. When the spring-loaded fixing seat 52132 is installed on the mounting surface 521113, the first guide groove 521112 and the second guide groove 521322 are arranged opposite each other and together form a guide cavity. The guide part 521311 is movably disposed in the first guide groove 521112 and the second guide groove 521322.

[0230] Furthermore, the spring-loaded fixing seat 52132 is provided with a movable groove 521321, which is connected to the second guide groove 521322. The pressing part 521312 passes through the spring-loaded fixing seat 52132 through the movable groove 521321.

[0231] Furthermore, the spring-loaded fixing seat 52132 is provided with a fastening groove 521323, and the spring-loaded fixing seat 52132 can be fastened to the storage mounting seat 5211 through the fastening groove 521323.

[0232] like Figure 26 , Figure 27 and Figure 29 As shown, the spring-loaded fixing seat 52132 includes a fixing part 521324 and a protrusion 521325. The fixing part 521324 is fixedly installed on the mounting surface 521113. The protrusion 521325 protrudes from the fixing part 521324 in the direction close to the consumable box 6. The pressing part 521312 passes through the protrusion 521325. Specifically, the movable groove 521321 is formed on the protrusion 521325, and the fastening groove 521323 is formed on the fixing part 521324.

[0233] Furthermore, a fixing groove 521111 is provided on the storage mounting base 521111, and the fixing part 521324 is installed in the fixing groove 521111. Specifically, a fixing groove 521111 is provided on the mounting surface 521113, and the fixing part 521324 is placed in the fixing groove 521111 to limit the movement; a connecting groove is provided on the bottom wall of the fixing groove 521111. When the fixing part 521324 is placed in the fixing groove 521111, the connecting groove and the fastening groove 521323 are aligned and connected, and the fastener 5215 connects the storage mounting base 5211 and the spring-loaded fixing base 52132 through the connecting groove and the fastening groove 521323.

[0234] like Figures 30-32As shown, this embodiment also provides a low-temperature storage mechanism 52, which includes an anti-condensation component 521, a refrigeration component 523, and a storage drive component 522. The refrigeration component 523 includes a support frame 5231 and a refrigeration element 5232. A consumable box 6 is placed on the support frame 5231, and the refrigeration element 5232 refrigerates the consumable box 6. The storage drive component 522 drives the storage mounting base 5211 to move up and down relative to the support frame 5231. When the storage drive assembly 522 drives the storage mounting base 5211 to move downward, the pressure cap component 5212 and the spring-loaded component 5213 move downward along with the storage mounting base 5211. The second ends of both the pressure cap component 5212 and the spring-loaded component 5213 abut against the sealing surface of the consumable box 6. That is, the pressure cap component 5212 is in the first state, which can prevent condensate from entering the consumable box 6 from the sealing surface. When the storage drive assembly 522 drives the storage mounting base 5211 to rise, the pressure cap component 5212 moves upward along with the storage mounting base 5211, so that the pressure cap component 5212 moves away from the sealing surface of the consumable box 6. However, the second end of the spring-loaded component 5213 extends beyond the pressure cap component 5212 and still abuts against the sealing surface of the consumable box 6. That is, the pressure cap component 5212 is in the second state, which prevents the consumable box 6 from moving with the pressure cap component 5212, so as to prevent damage to the consumable box 6 and the refrigerated items on the consumable box 6, extend the service life of the consumable box 6, and ensure the safety of low-temperature storage.

[0235] Of course, in other embodiments, the storage drive component 522 may also drive the storage mounting base 5211 to rotate or translate on the horizontal plane. The movement of the storage mounting base 5211 is not limited, as long as the anti-condensation component 521 can be covered or moved away from the consumable box 6.

[0236] In this embodiment, the low-temperature storage mechanism 52 further includes a housing 524, which covers the anti-condensation component 521, the storage drive component 522, and the refrigeration component 523.

[0237] Furthermore, the storage drive assembly 522 includes a storage drive component 5222 and a storage drive board 5221. The storage drive component 5222 is fixedly mounted on the housing 524. The storage drive component 5222 drives the storage drive board 5221 to move up and down relative to the support frame 5231. The anti-condensation component 521 is mounted on the storage drive board 5221. Specifically, the storage mounting base 5211 is fixedly mounted on the storage drive board 5221.

[0238] Furthermore, the storage mounting base 5211 includes a mounting portion 52111 and a connecting portion 52112. The mounting portion 52111 and the connecting portion 52112 are integrally formed, and the connecting portion 52112 is located at the end of the mounting portion 52111. The mounting surface 521113 is disposed on the mounting portion 52111, and the connecting portion 52112 is fixedly mounted on the storage drive plate 5221. The storage drive component 5222 drives the storage drive plate 5221 to descend, and the storage drive plate 5221 drives the storage mounting base 5211, the pressure cover component 5212, and the spring buffer component 5213 to descend, so that the elastic pad 52122 in the pressure cover component 5212 and the spring buffer component 52131 in the spring buffer component 5213 both press against the sealing surface of the consumable box 6. During this process, the elastic component 52133 is in a compressed state. The storage drive component 5222 drives the storage drive plate 5221 to rise, thereby driving the storage mounting base 52111 to rise. As the mounting base 5211 and the pressure cap component 5212 rise, the elastic pad 52122 of the pressure cap component 5212 moves away from the sealing surface of the consumable box 6, making it easier to take out and put in the refrigerated items on the consumable box 6. During this process, since the elastic element 52133 is in a compressed state, the spring-loaded element 52131 is still pressed against the sealing surface of the consumable box 6, preventing the consumable box 6 from moving with the pressure cap component 5212, so as to prevent damage to the consumable box 6 and the refrigerated items on the consumable box 6, extend the service life of the consumable box 6, and ensure the safety of low-temperature storage.

[0239] Furthermore, the storage drive assembly 522 also includes a mounting plate 5223, which is fixedly mounted on the inner wall of the housing 524, and the storage drive 5222 is mounted on the mounting plate 5223.

[0240] Furthermore, the storage driver assembly 522 also includes a connecting block 5224, the output end of the storage driver 5222 is connected to the connecting block 5224, and the storage driver board 5221 is mounted on the connecting block 5224.

[0241] In this embodiment, the number of support frames 5231 can be multiple, and the multiple support frames 5231 can be arranged in rows and columns to facilitate the low-temperature storage of more consumable boxes 6. This embodiment does not limit the specific number of support frames 5231 in each row and column, and can set it according to the actual situation.

[0242] In this embodiment, the number of cooling components 5232 is not limited. The cooling components 5232 can cool all the consumable boxes 6, or the cooling components 5232 can be set one-to-one with the consumable boxes 6, as long as a good cooling effect can be achieved.

[0243] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. An automated integrated machine for NGS library preparation, hybridization, sequencing, and interpretation, used for adding samples to and sequencing sequencing chips (32), characterized in that, The system includes a rack (1), a robotic arm (2) mounted on the rack (1), a sequencing device (3), and a library preparation instrument (4). The sequencing device (3) includes a gene sequencer (31), a sample clamping mechanism (33), and a flipping mechanism (34). The library preparation instrument (4) is capable of adding samples to the sequencing chip (32). The gene sequencer (31) has a slot (311) for inserting the sequencing chip (32); The sample loading clamping mechanism (33) includes a clamping base (336) and a clamping fixture (330). The clamping base (336) is provided with a second positioning groove (3361). The clamping fixture (330) is placed in the second positioning groove (3361). The clamping fixture (330) has an open state and a closed state. When the clamping fixture (330) comes into contact with the gene sequencer (31), it will trigger the clamping fixture (330) to switch from the closed state to the open state. When the clamping fixture (330) is in the closed state, the clamping fixture (330) clamps the sequencing chip (32). A flipping mechanism (34) is capable of flipping the clamping fixture (330) to drive the sequencing chip (32) after sample loading to flip; The robotic arm (2) includes an execution end (21) and a clamping member (22). The clamping member (22) is located on the execution end (21). The clamping member (22) can clamp the clamping base (336) or the clamping fixture (330). The execution end (21) can move between the library preparation instrument (4), the flipping mechanism (34), and the gene sequencer (31) so that the sequencing chip (32) is sampled, flipped, and inserted into the preset position of the slot (311).

2. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 1, characterized in that, The clamping fixture (330) includes: The fixing component (331) includes a fixing member (3311) and a locking positioning member (3312). The fixing member (3311) has an installation groove (3310), and the locking positioning member (3312) is disposed in the installation groove (3310). The locking positioning member (3312) is provided with a positioning part (33121) for positioning the sequencing chip (32). The top cover (332), one end of which is rotatably connected to the fastener (3311), is used to switch between opening or closing the mounting slot (3310); A reset component (333) is disposed between the upper cover (332) and the fixing component (331), the reset component (333) being used to automatically open the unlocked upper cover (332); A locking assembly (334) is disposed between the upper cover (332) and the fixing assembly (331), the locking assembly (334) being used to lock the closed upper cover (332); When the top cover (332) is closed, the sequencing chip (32) is held between the locking positioning member (3312) and the top cover (332).

3. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 2, characterized in that, The locking assembly (334) includes: A first locking connector (3341) is disposed on the upper cover (332); The second locking connector (3342) is disposed on the fixing component (331). The first locking connector (3341) and the second locking connector (3342) can be engaged or disengaged. When the second locking connector (3342) contacts the gene sequencer (31), the first locking connector (3341) and the second locking connector (3342) are disengaged.

4. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 3, characterized in that, The first locking connector (3341) includes a hook (33411), one end of which is connected to the upper cover (332); The second locking connector (3342) includes a hook connector (33421). A sliding groove (33112) is provided on the fixing member (3311) below the locking positioning member (3312). One end of the hook connector (33421) is slidably disposed in the sliding groove (33112). The hook connector (33421) has a slot (334211). When the second locking connector (3342) contacts the gene sequencer (31), one end of the hook connector (33421) slides in the sliding groove (33112) so that the other end of the hook (33411) disengages from the slot (334211).

5. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 4, characterized in that, The locking assembly (334) further includes a locking spring (3343), which is used to keep the hook (33411) and the slot (334211) in a locked state. One end of the hook connector (33421) is connected to one end of the locking spring (3343), and the other end of the locking spring (3343) is connected to the groove wall of the sliding groove (33112).

6. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 2, characterized in that, The upper cover (332) and the fixing member (3311) are rotatably connected by a rotating shaft (338), and the reset assembly (333) includes: A torsion spring mounting component (3331) is rotatably mounted on the rotating shaft (338), and the torsion spring mounting component (3331) is connected to the upper cover (332); A torsion spring (3332) is sleeved on the rotating shaft (338) and mounted on the torsion spring mounting member (3331).

7. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 2, characterized in that, The sample loading clamping mechanism (33) also includes a sample loading seat (337), the sequencing chip (32) is provided with a sample loading port (322), the sample loading seat (337) is located on one side of the sample loading port (322), and the clamping fixture (330) is located on the opposite side of the sample loading seat (337); The sequencing chip (32) has a side positioning protrusion (323) on the side wall of the end that extends out of the clamping fixture (330); The sample loading seat (337) is placed on the clamping base (336), and the sample loading seat (337) is provided with an open slot (3371). The side positioning protrusion (323) is engaged in the open slot (3371).

8. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 1, characterized in that, The robotic arm (2) further includes a chip pushing mechanism (23), which is connected to the execution end (21) and is used to push the stuck sequencing chip (32) to a preset position in the slot (311). The chip pushing mechanism (23) includes: Push the base (231); A Z-axis adjustment assembly (232) is disposed on one side of the push base (231), the Z-axis adjustment assembly (232) including a Z-axis moving component (2321) movable in the Z direction; and A pushing component (233) is fixedly disposed on the side of the Z-axis moving component (2321) away from the pushing base (231). The pushing component (233) includes: A pushing surface (2331) is located on the side of the pushing component (233) away from the Z-direction moving component (2321), and the pushing surface (2331) is perpendicular to the X-direction; A first actuating block (2332) extends from one end of the pushing surface (2331) in the Z direction along the X direction and away from the Z-direction moving member (2321); and The second actuating block (2333) extends from the pushing surface (2331) at the other end in the Z direction along the X direction and away from the Z-direction moving member (2321).

9. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 1, characterized in that, The flipping mechanism (34) includes: A flip drive assembly (342) includes a rotary drive component (3421) and a clamping drive component (3422) connected to each other. The rotary drive component (3421) is configured to drive the clamping drive component (3422) to rotate about a rotation axis (3423). The clamping drive component (3422) has a first actuating end (34221) and a second actuating end (34222), at least one of the first actuating end (34221) and the second actuating end (34222) being configured to be movable between a clamping position and a releasing position. A first gripper (343) is disposed at the first execution end (34221); and The second gripper (344) is disposed at the second actuating end (34222). A clamping area is formed between the first gripper (343) and the second gripper (344). A first clamping positioning member is provided on the side of the first gripper (343) facing the clamping area, and a second clamping positioning member (3441) is provided on the side of the second gripper (344) facing the clamping area.

10. The fully automated integrated machine for NGS library construction, hybridization, sequencing, and interpretation according to claim 1, characterized in that, The fully automated NGS library construction, hybridization, sequencing, and interpretation system also includes a low-temperature storage mechanism (52), which comprises: An anti-condensation component (521) includes a storage mounting base (5211), a pressure cap component (5212), and a spring-loaded component (5213). The storage mounting base (5211) has a mounting surface (521113). The pressure cap component (5212) is mounted on the mounting surface (521113). The first end of the spring-loaded component (5213) is mounted on the mounting surface (521113). The pressure cap component (5212) has a first state and a second state. When the pressure cap component (5212) is in the first state, the second ends of both the pressure cap component (5212) and the spring-loaded component (5213) abut against the sealing surface of the consumable box (6); when the pressure cap component (5212) is in the second state, the pressure cap component (5212) is away from the sealing surface of the consumable box (6), and the second end of the spring-loaded component (5213) extends beyond the pressure cap component (5212) and abuts against the sealing surface of the consumable box (6); A refrigeration assembly (523) includes a support frame (5231) and a refrigeration component (5232), wherein a consumable box (6) is placed on the support frame (5231), and the refrigeration component (5232) refrigerates the consumable box (6); A storage drive assembly (522) drives the storage mounting base (5211) to move up and down relative to the support frame (5231).

Images