Biological gene extraction device for rice inspection and quarantine

Abstract

The invention belongs to the technical field of extraction devices, and particularly relates to a biological gene extraction device for rice inspection and quarantine, which comprises a centrifugal extraction tank for centrifugally separating samples and a high-pressure top cover correspondingly fixed at the top position of the centrifugal extraction tank, a separation groove for separating a cell carrier is fixedly mounted at the position of an output shaft of the centrifugal base, and a pressurizing mechanism for adjusting the pressure intensity of the inner side of the centrifugal extraction tank is arranged at the position of the top of the inner side of the centrifugal extraction tank; according to the scheme, the pressurizing device is correspondingly arranged on the inner side of the centrifugal extraction tank, so that the pressurizing device and the pressure-resistant inner container synchronously rotate for pressurizing, and the breakage speed of cell walls of extracted cells is effectively accelerated. And to a certain extent, when the device is adapted to other experiments, the fusion of the extract can be effectively accelerated.

Description

Technical Field

[0001] The invention belongs to the technical field of extraction devices, and in particular relates to a biological gene extraction device for rice inspection and quarantine. Background Art

[0002] Rice is one of the most important food crops in the world, and about half of the world's population relies on rice as their staple food. Rice bacterial blight, also known as white leaf blight, thatch blight, ground fire, etc., is one of the most important rice diseases, which has a serious impact on rice production. The disease is caused by bacteria, and the pathogen is Xanthomonas oryzae.

[0003] The existing biological gene extraction device for rice inspection and quarantine adopts static sampling, and performs segregation, rotation, centrifugal and wall breaking processing on the rice cell samples through step-by-step oscillation and pressurization, so as to achieve the extraction of quarantine cells. After long-term extraction experiments, it was found that the effect of using centrifugal wall breaking is best under high-pressure vibration environment. At the same time, it can greatly avoid the contact between thin-walled cell tissue and the external environment of detection, and ensure the survival rate of extraction. For this reason, we propose a biological gene extraction device for rice inspection and quarantine. Summary of the invention

[0004] The purpose of the present invention is to provide a biological gene extraction device for rice inspection and quarantine to solve the problems raised in the above background technology.

[0005] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a biological gene extraction device for rice inspection and quarantine, comprising a centrifugal extraction tank for centrifugal separation of samples, and a high-pressure top cover fixed at the top position of the centrifugal extraction tank, wherein a centrifugal base is rotatably installed at the inner bottom position of the centrifugal extraction tank, a separation groove for separating cell carriers is fixedly installed at the output shaft position of the centrifugal base, and a pressurizing mechanism for adjusting the inner pressure of the centrifugal extraction tank is provided at the inner top position of the centrifugal extraction tank; The pressurizing mechanism comprises a pressurizing top plate, one side of which is fixedly connected to the pressurizing device via a transfer tube, and a sealed pressurizing turntable for pressure adjustment is rotatably installed on the inner bottom of the pressurizing top plate, and a pressure detecting device for detecting pressure is arranged at the middle position of the sealed pressurizing turntable, wherein a pressure supply groove is provided at the edge position of the inner side of the sealed pressurizing turntable corresponding to the pressure detecting device, and the bottom end of the pressure supply groove is docked and fixed to the pressurizing top cover via a docking valve, and the pressurizing top cover is fixed correspondingly at the top position of the cell carrier, so as to directionally pressurize the sample loaded on the inner side of the cell carrier.

[0006] Furthermore, a boosting main pipe for docking with a docking valve is provided at the top center position of the boosting top cover, and a plurality of pressure-stabilizing branch pipes penetrating the top of the boosting top cover are provided in a ring shape on the outer bottom of the boosting main pipe, wherein a boosting plate is vertically slidably provided on the inner side of the boosting top cover, and a boosting piston is fixedly installed at a position directly below the cavity of the boosting main pipe corresponding to the top of the boosting plate, and a pressure-stabilizing piston is fixedly installed at a position directly below the cavity of the pressure-stabilizing branch pipe corresponding to the top of the boosting plate, wherein the boosting piston and the outer side of the top end of each pressure-stabilizing piston are tightly fitted with a sealing rubber ring sleeve for sealing.

[0007] Furthermore, the bottom end of the pressure-stabilizing branch pipe penetrates the bottom wall of the booster cover and is fixedly connected to the extension sleeve, wherein the pressure-stabilizing piston slides vertically along the inner side wall of the extension sleeve.

[0008] Furthermore, a special-shaped sealing sleeve is provided on the outer side of the supercharging plate, wherein the special-shaped sealing sleeve is a double-layer structure.

[0009] Furthermore, an extended edge is fixedly installed at the top of the cell carrier, and a docking fastener used in conjunction with the pressurized top cover is arranged on the outer side of the extended edge.

[0010] Furthermore, dividing grooves are laterally distributed at the middle of the inner side of the cell carrier.

[0011] Furthermore, one side of the centrifugal extraction tank is provided with a slot structure for placing a pressurizing device.

[0012] Furthermore, a pressure-resistant inner liner is detachably provided on the inner side of the centrifugal extraction tank.

[0013] Furthermore, a blocking protrusion is fixedly installed at the end position of the dividing groove.

[0014] Furthermore, a pressure control valve is fixedly installed at the top position of the high-pressure top cover, and a sealing ring structure is filled and arranged at the docking position between the high-pressure top cover and the centrifugal extraction tank.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. In this scheme, the booster device is installed on the inner side of the centrifugal extraction tank, so that it rotates and boosts pressure synchronously with the pressure-resistant inner tank, which effectively accelerates the cell wall damage rate of the extracted cells. And to a certain extent, when adapting to other experiments, it can also effectively accelerate the fusion of the extract.

[0016] 2. By correspondingly setting the pressure control valve on the top of the high-pressure top cover and cooperating with the pressure-boosting adjustment of the pressure-boosting device, the negative pressure state of the cell sample inside the cell carrier of the device can be effectively changed. In addition, the pressure-stabilizing branch pipe is used in conjunction with the pressure-stabilizing effect of the pressure-boosting main pipe to ensure the stability of the pressure-boosting plate during the lifting and lowering adjustment process, thereby reducing the situation of unstable pressure in the chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention.

[0018] Figure 1 It is a schematic diagram of the overall partial cross-sectional structure of the present invention; Figure 2 It is a schematic diagram of the overall structure of the present invention; Figure 3 It is a schematic diagram of the combined structure of the supercharged top plate and the supercharged top cover of the present invention; Figure 4 For the present invention Figure 3 Schematic diagram of the split structure; Figure 5 This is a schematic diagram of the cell support structure of the present invention; Figure 6 It is a schematic diagram of a partial cross-sectional structure of a supercharging top cover of the present invention; In the figure: 1. Centrifugal extraction tank; 11. Pressure-resistant inner tank; 12. Centrifugal base; 13. Dividing groove; 14. Grid protrusion; 2. High-pressure top cover; 21. Pressure control valve; 3. Booster top plate; 31. Transfer tube; 32. Booster device; 33. Sealed booster turntable; 34. Pressure detection device; 35. Pressure supply groove; 4. Booster top cover; 41. Booster main pipe; 42. Pressure stabilizing branch pipe; 43. Docking valve; 44. Extension sleeve; 45. Booster piston; 46. Pressure stabilizing piston; 47. Sealing rubber ring; 48. Booster plate; 49. Special-shaped sealing sleeve; 5. Cell carrier; 51. Docking fastener; 52. Dividing groove; 53. Extended edge. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0020] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0021] Reference Figure 1 - Figure 6 The present invention proposes a technical solution: a biological gene extraction device for rice inspection and quarantine, comprising a centrifugal extraction tank 1 for centrifugal separation of samples, and a high-pressure top cover 2 correspondingly fixed at the top position of the centrifugal extraction tank 1, wherein a pressure control valve 21 is fixedly installed at the top position of the high-pressure top cover 2, a centrifugal base 12 is rotatably installed at the inner bottom position of the centrifugal extraction tank 1, a separation groove 13 for separating cell carriers 5 is fixedly installed at the output shaft position of the centrifugal base 12, and a pressurizing mechanism for adjusting the inner pressure of the centrifugal extraction tank 1 is provided at the inner top position of the centrifugal extraction tank 1; By correspondingly setting the pressure control valve 21 on the top of the high-pressure top cover 2 and cooperating with the pressure-boosting adjustment of the pressure-boosting device 32, the negative pressure state of the cell sample carrier inside the cell carrier 5 of the device can be effectively changed, and the pressure-stabilizing effect of the pressure-stabilizing branch pipe 42 and the pressure-stabilizing main pipe 41 is utilized to ensure the stability of the pressure-boosting plate 48 during the lifting and lowering adjustment process, thereby reducing the situation of unstable pressure in the chamber.

[0022] The pressurizing mechanism includes a pressurizing top plate 3, one side of the pressurizing top plate 3 is fixedly connected to the pressurizing device 32 through a transfer tube 31, and a sealed pressurizing turntable 33 for pressure adjustment is rotatably installed on the inner bottom of the pressurizing top plate 3, and a pressure detection device 34 for detecting pressure is arranged at the middle position of the sealed pressurizing turntable 33, wherein a pressure supply groove 35 is opened at the edge position of the inner side of the sealed pressurizing turntable 33 corresponding to the pressure detection device 34, and the bottom end of the pressure supply groove 35 is docked and fixed to the pressurizing top cover 4 through a docking valve 43, and the pressurizing top cover 4 is fixed correspondingly at the top position of the cell carrier 5, for directional pressurization of the sample loaded on the inner side of the cell carrier 5.

[0023] A booster main pipe 41 for docking with the docking valve 43 is arranged at the top center of the booster top cover 4, and a plurality of pressure-stabilizing branch pipes 42 penetrating the top of the booster top cover 4 are annularly opened at the outer bottom of the booster main pipe 41, wherein a booster plate 48 is vertically slidably arranged on the inner side of the booster top cover 4, and a booster piston 45 is fixedly installed at the top of the booster plate 48 corresponding to the position directly below the cavity of the booster main pipe 41, and a pressure-stabilizing piston 46 is fixedly installed at the top of the booster plate 48 corresponding to the position directly below the cavity of the pressure-stabilizing branch pipe 42, wherein the top outer surface of the booster piston 45 and each pressure-stabilizing piston 46 The sides are tightly sleeved with a sealing rubber ring 47 for sealing, the bottom end of the pressure-stabilizing branch pipe 42 passes through the bottom wall of the boosting top cover 4 and is fixedly connected to the extension sleeve 44, wherein the pressure-stabilizing piston 46 slides vertically along the inner wall of the extension sleeve 44, and the outer side of the boosting plate 48 is sleeved with a special-shaped sealing sleeve 49, wherein the special-shaped sealing sleeve 49 is a double-layer structure, an extension edge 53 is fixedly installed at the top position of the cell carrier 5, and a docking fastener 51 used in conjunction with the boosting top cover 4 is arranged on the outer side of the extension edge 53, and a dividing groove 52 is horizontally distributed at the inner middle position of the cell carrier 5; It should be noted that two types of docking fasteners 51 are proposed to ensure the stability of the docking between the pressurized top cover 4 and the cell support dish 5. Specifically, they can be fixed directly by screws or by spring fasteners to ensure the stable fit between the two.

[0024] One side of the centrifugal extraction tank 1 is provided with a slot structure for placing the booster device 32 , thereby ensuring the stability of the booster device 32 in the working state.

[0025] In order to ensure the safety of the experiment, a pressure-resistant inner liner 11 is detachably provided on the inner side of the centrifugal extraction tank 1, which can be disassembled and fixed by screws.

[0026] A grid protrusion 14 is fixedly installed at the end position of the dividing groove 13, wherein a sealing ring structure is filled and arranged at the butt joint position between the high-pressure top cover 2 and the centrifugal extraction tank 1.

[0027] The working principle and use process of the present invention: when using this type of biological gene extraction device for rice inspection and quarantine, taking the cell extraction of rice bacterial blight as an example, the rice bacterial blight bacteria after cleaning and disinfection are placed in the cell carrier 5 as they are, and the corresponding buckle is set at the bottom of the booster top cover 4. It should be noted that the booster reaction during the separation cell wall crushing experiment is referred to. Before use, it is necessary to ensure that the booster plate 48 is placed at the inner top position of the booster top cover 4 to provide a certain negative pressure space. After the adjustment is completed, the booster main pipe 41 and the docking valve 43 are docked and fixed on the pressure supply pipe distributed at the bottom of the pressure supply groove 35, and then the negative pressure test is carried out through the booster device 32. After ensuring the basic negative pressure supply, the high-pressure top cover 2 is closed accordingly to complete the pre-test adjustment; The centrifugal base 12 is used to drive the cell carrier 5 to rotate, and then the pressure supply groove 35 is pressurized and adjusted through the boosting device 32. The air pressure applies force to the boosting piston 45 to move downward through the boosting main pipe 41. When the boosting piston 45 moves downward, it drives the boosting plate 48 to supply pressure to the inside of the cell carrier 5. Under the premise of ensuring the sealing of the docking position between the boosting top cover 4 and the cell carrier 5, the cell wall inside the cell carrier 5 is pressurized and broken. At the same time, due to the effect of centrifugal rotation, the synchronous operation of boosting and centrifugation can be effectively realized, and the biological genes of the sampled cells can be quickly accelerated.

[0028] According to the above working process, it can be known that in this solution, the booster device correspondingly arranged inside the centrifugal extraction tank 1 is used to rotate and boost pressure synchronously with the pressure-resistant inner liner 11, which effectively accelerates the cell wall damage rate of the extracted cells. And to a certain extent, when adapted to other experiments, it can also effectively accelerate the fusion of the extract.

[0029] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A biological gene extraction device for rice inspection and quarantine, comprising a centrifugal extraction tank (1) for centrifugally separating samples, and a high-pressure top cover (2) fixed to the top of the centrifugal extraction tank (1), wherein a centrifugal base (12) is rotatably mounted at the bottom of the inner side of the centrifugal extraction tank (1), characterized in that: A separation groove (13) for separating the cell carriers (5) is fixedly installed at the output shaft position of the centrifugal base (12), and a pressurizing mechanism for adjusting the pressure inside the centrifugal extraction tank (1) is provided at the inner top position of the centrifugal extraction tank (1); The pressurizing mechanism comprises a pressurizing top plate (3), one side of the pressurizing top plate (3) is fixedly connected to the pressurizing device (32) via a transfer tube (31), and a sealed pressurizing turntable (33) for pressure adjustment is rotatably mounted on the bottom of the inner side of the pressurizing top plate (3), and a pressure detection device (34) for detecting pressure is arranged at the middle position of the sealed pressurizing turntable (33), wherein a pressure supply groove (35) is provided at the edge position of the inner side of the sealed pressurizing turntable (33) corresponding to the pressure detection device (34), and the bottom end of the pressure supply groove (35) is docked and fixed to the pressurizing top cover (4) via a docking valve (43), and the pressurizing top cover (4) is correspondingly fixed at the top position of the cell carrier (5) for directional pressurization of the sample loaded on the inner side of the cell carrier (5).

2. The biological gene extraction device for rice inspection and quarantine according to claim 1, characterized in that: A boosting main pipe (41) for docking with a docking valve (43) is arranged at the top center of the boosting top cover (4), and a plurality of pressure-stabilizing branch pipes (42) penetrating the top of the boosting top cover (4) are annularly opened at the outer bottom of the boosting main pipe (41), wherein a boosting plate (48) is vertically slidably arranged on the inner side of the boosting top cover (4), and a boosting piston (45) is fixedly installed at the top of the boosting plate (48) corresponding to a position directly below the cavity of the boosting main pipe (41), and a pressure-stabilizing piston (46) is fixedly installed at the top of the boosting plate (48) corresponding to a position directly below the cavity of the pressure-stabilizing branch pipe (42), wherein the outer sides of the top ends of the boosting piston (45) and each pressure-stabilizing piston (46) are tightly fitted with a sealing rubber ring (47) for sealing.

3. The biological gene extraction device for rice inspection and quarantine according to claim 2, characterized in that: The bottom end of the pressure-stabilizing branch pipe (42) penetrates the bottom wall of the booster cover (4) and is fixedly connected to the extension sleeve (44), wherein the pressure-stabilizing piston (46) slides vertically along the inner side wall of the extension sleeve (44).

4. The biological gene extraction device for rice inspection and quarantine according to claim 2, characterized in that: The outer side of the boost plate (48) is sleeved with a special-shaped sealing sleeve (49).

5. The biological gene extraction device for rice inspection and quarantine according to claim 2, characterized in that: An extended edge (53) is fixedly mounted at the top of the cell carrier (5), and a docking fastener (51) for use with the pressurized top cover (4) is arranged on the outer side of the extended edge (53).

6. The biological gene extraction device for rice inspection and quarantine according to claim 5, characterized in that: A dividing groove (52) is laterally distributed at the middle position of the inner side of the cell carrier (5).

7. The biological gene extraction device for rice inspection and quarantine according to claim 1, characterized in that: One side of the centrifugal extraction tank (1) is provided with a slot structure for placing a pressure boosting device (32).

8. The biological gene extraction device for rice inspection and quarantine according to claim 1, characterized in that: A pressure-resistant inner liner (11) is detachably provided on the inner side of the centrifugal extraction tank (1).

9. The biological gene extraction device for rice inspection and quarantine according to claim 1, characterized in that: A blocking protrusion (14) is fixedly mounted at the end position of the dividing groove (13).

10. The biological gene extraction device for rice inspection and quarantine according to claim 1, characterized in that: A pressure control valve (21) is fixedly installed at the top position of the high-pressure top cover (2), and a sealing ring structure is filled and arranged at the butt joint position between the high-pressure top cover (2) and the centrifugal extraction tank (1).

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