Automatic excitation light source device and use method thereof

Abstract

According to the automatic excitation light source device and the use method thereof, an excitation light source and related devices are directly installed in a detector, and test, regulation and control of excitation light source parameters can be conveniently achieved through specific software. For example, the time interval from turning off the light source to starting to collect the delayed light-emitting signal is accurately controlled, the collection period is circularly set, and the power of the light source is adjusted. In short, the automatic excitation light source device and the use method thereof are reasonable in structural design, convenient and stable to operate and suitable for popularization and use.

Description

Technical Field

[0001] The present invention relates to an automatic excitation light source device and a use method thereof, belonging to the field of biophoton technology. Background Art

[0002] Although all organisms in nature can emit biophoton signals, most organisms' life activities are relatively weak, resulting in low photon emission intensities. This makes it difficult to set a very short sampling interval (typically 1 second or longer) when collecting their spontaneous luminescence signals. Consequently, as the length of the sample signal increases and the number of samples increases, the time cost of collecting sample spontaneous luminescence signal data becomes prohibitive. Furthermore, spontaneous luminescence signals are highly susceptible to the influence of the experimental environment; once environmental conditions change, valid photon signal data acquisition becomes difficult. As a typical open living system, organisms continuously exchange matter and energy with the external environment. When stimulated by external light sources, organisms continuously absorb energy and exhibit a specific relaxation radiation behavior after the excitation process ends. However, when the existing excitation light source device excites the sample to be tested, it is impossible to accurately control the time interval from the light source being turned off to the delayed luminescence signal collection; at the same time, since the currently used excitation light source is directly welded in the circuit, it is not easy to disassemble or replace other light sources. When you want to replace other monochromatic light sources, you can only use corresponding filters or design the circuit separately. This not only increases the workload of collecting delayed luminescence signals, but also greatly affects the efficiency and effect of subsequent signal collection. Summary of the Invention

[0003] In order to overcome the defects existing in the background technology, the technical solution adopted by the present invention to solve its technical problems is: an automatic excitation light source device, including an operating table, a control computer, a controller and a base, the control computer, the controller and the base are placed on the operating table, the base is provided with a height adjustment screw and a scale, the lower part of the height adjustment screw is movably connected to the base through a thread, the top of the height adjustment screw is provided with a universal ball placement groove, a rotatable universal ball is placed in the universal ball placement groove, a universal ball locking bolt is provided on the universal ball placement groove, the universal The ball is fixedly connected to the motor fixing plate through a connecting rod, a connecting rod placement slot is provided on the universal ball placement slot corresponding to the connecting rod, a motor is fixed on the motor fixing plate, a light source placement turntable is provided below the motor fixing plate, the motor can drive the light source placement turntable to rotate, a plurality of excitation light sources are provided below the light source placement turntable, a sample placement table is provided on the base corresponding to the excitation light source, a quartz cup placement slot is provided on the sample placement table, the sample placement table and a height adjustment screw are arranged in a protective cover, the protective cover is placed on the base, and an upper cover is provided on the top of the protective cover.

[0004] Preferably, the light source is placed below the turntable and six excitation light sources, namely, excitation light source 1, excitation light source 2, excitation light source 3, excitation light source 4, excitation light source 5 and excitation light source 6, are arranged at equal intervals.

[0005] Preferably, the first excitation light source is a white light source; the second excitation light source is a purple light source; the third excitation light source is a green light source; the fourth excitation light source is a yellow light source; the fifth excitation light source is a red light source; and the sixth excitation light source is a blue light source.

[0006] Preferably, the protective cover and the upper cover are both made of opaque materials.

[0007] Preferably, the middle portion of the height-adjusting screw is hollow.

[0008] Preferably, the upper cover is movably arranged on the top of the protective cover via a hinge. A handle is provided above the upper cover.

[0009] The method for using the automatic excitation light source device comprises the following steps: (1) Open the upper cover on the top of the protective cover and place the quartz cup containing the sample to be excited by the light source into the quartz cup placement slot on the sample placement table; (2) Turn the height adjustment screw to adjust the distance between the light source turntable and the quartz cup; (3) Move the connecting rod up and down to adjust the angle of the light source placement turntable to the base, turn the universal ball locking bolt, and clamp the universal ball into the universal ball placement slot; (4) Cover the top cover of the protective cover; (5) Select the desired color of the excitation light source on the control computer, and the controller will control the motor to drive the light source placement turntable to rotate so that the excitation light source of the desired color is aligned with the quartz cup; (6) After setting the intensity parameters, excitation time parameters, sampling interval, excitation cycle and other parameters of the excitation light source on the control computer, the automatic excitation light source device can start working and complete the excitation irradiation of the sample by the excitation light source.

[0010] The present invention provides an automated excitation light source device and its use method. This device and its use method primarily install the excitation light source and related equipment directly within a detector, facilitating the testing and control of the excitation light source parameters using specialized software. For example, this device can precisely control the time interval between shutting off the light source and starting to collect delayed luminescence signals, set the acquisition cycle, and adjust the light source power. Overall, this automated excitation light source device and its use method boast a rational design, convenient and stable operation, and are suitable for widespread use. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] The present invention will be further described below with reference to the accompanying drawings and examples.

[0012] Figure 1 It is a structural schematic diagram of an automatic excitation light source device of the present invention; Figure 2 This is a schematic diagram of the internal structure of an automatic excitation light source device of the present invention. Figure 1 ; Figure 3 This is a schematic diagram of the internal structure of an automatic excitation light source device of the present invention. Figure 2 ; Figure 4 yes Figure 1 A partial enlarged view of point A in the middle; Among them: 1. Operating table; 2. Control computer; 3. Controller; 4. Base; 5. Protective cover; 6. Upper cover; 61. Hinge; 7. Handle; 8. Sample placement table; 81. Quartz cup placement slot; 9. Height adjustment screw; 10. Universal ball placement slot; 11. Universal ball; 12. Universal ball locking bolt; 13. Connecting rod; 14. Connecting rod placement slot; 15. Motor fixing plate; 16. Motor; 17. Light source placement turntable; 181. Excitation light source one; 182. Excitation light source two; 183. Excitation light source three; 184. Excitation light source four; 185. Excitation light source five; 186. Excitation light source six; 19. Scale. DETAILED DESCRIPTION

[0013] The present invention will now be described in further detail with reference to the accompanying drawings, which are simplified schematic diagrams that illustrate the basic structure of the present invention in a schematic manner, and thus only show components related to the present invention.

[0014] For specific embodiment 1, please refer to Figure 1-4, an automated excitation light source device, comprising an operating table 1, a control computer 2, a controller 3 and a base 4, wherein the control computer 2, the controller 3 and the base 4 are placed on the operating table 1, and a height adjustment screw 9 and a scale 19 are provided on the base 4, the scale 19 is used to display the specific height of the height adjustment screw 9, the lower part of the height adjustment screw 9 is movably connected to the base 4 through a thread, a universal ball placement groove 10 is provided on the top of the height adjustment screw 9, a rotatable universal ball 11 is placed in the universal ball placement groove 10, a universal ball locking bolt 12 is provided on the universal ball placement groove 10, and the universal ball 11 is fixed to the motor through a connecting rod 13 The fixed plates 15 are fixedly connected together, a connecting rod placement slot 14 is provided on the universal ball placement slot 10 corresponding to the connecting rod 13, a motor 16 is fixed on the motor fixing plate 15, a light source placement turntable 17 is provided below the motor fixing plate 15, the motor 16 can drive the light source placement turntable 17 to rotate, a plurality of excitation light sources are provided below the light source placement turntable 17, a sample placement table 8 is provided on the base 4 corresponding to the excitation light source, a quartz cup placement slot 81 is provided on the sample placement table 8, the sample placement table 8 and the height adjustment screw 9 are arranged in the protective cover 5, the protective cover 5 is placed on the base 4, and the top of the protective cover 5 is provided with an upper cover 6.

[0015] The light source is placed below the turntable 17 and six excitation light sources, namely, excitation light source 1 181, excitation light source 2 182, excitation light source 3 183, excitation light source 4 184, excitation light source 5 185 and excitation light source 6 186, are evenly spaced. The excitation light source 1 181 is a white light source; the excitation light source 2 182 is a purple light source; the excitation light source 3 183 is a green light source; the excitation light source 4 184 is a yellow light source; the excitation light source 5 185 is a red light source; and the excitation light source 6 186 is a blue light source.

[0016] The protective cover 5 and the upper cover 6 are both made of opaque material. The middle part of the height adjustment screw is hollow, which is convenient for placing various cables. The upper cover 6 is movably set on the top of the protective cover 5 through a hinge 61, and a handle 7 is provided above the upper cover 6.

[0017] The method for using the automatic excitation light source device is characterized by the following steps: (1) Open the upper cover 6 on the top of the protective cover 5 and place the quartz cup containing the sample to be excited by the light source into the quartz cup placement groove 81 provided on the sample placement table 8; (2) Turn the height adjustment screw 9 to adjust the distance between the light source placement turntable 17 and the quartz cup; (3) Move the connecting rod 13 up and down to adjust the angle of the light source placement turntable 17 to the base 4, turn the universal ball locking bolt 12, and clamp the universal ball 11 into the universal ball placement slot 10; (4) Cover the top cover 6 on the top of the protective cover 5; (5) The color of the desired excitation light source is selected on the control computer 2, and the controller 3 controls the motor 16 to drive the light source placement turntable 17 to rotate so that the excitation light source of the desired color is aligned with the quartz cup; (6) After setting the intensity parameters, excitation time parameters, sampling interval, excitation cycle and other parameters of the excitation light source on the control computer 2, the automatic excitation light source device can start working and complete the excitation irradiation of the sample by the excitation light source.

[0018] The present invention provides an automated excitation light source device and its use method. This device and its use method primarily install the excitation light source and related equipment directly within a detector, facilitating the testing and control of the excitation light source parameters using specialized software. For example, this device can precisely control the time interval between shutting off the light source and starting to collect delayed luminescence signals, set the acquisition cycle, and adjust the light source power. Overall, this automated excitation light source device and its use method boast a rational design, convenient and stable operation, and are suitable for widespread use.

[0019] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. An automated excitation light source device, comprising an operating table (1), a control computer (2), a controller (3) and a base (4), wherein the control computer (2), the controller (3) and the base (4) are placed on the operating table (1), characterized in that: The base (4) is provided with a height adjustment screw (9) and a scale (19); the lower portion of the height adjustment screw (9) is movably connected to the base (4) via a thread; the top of the height adjustment screw (9) is provided with a universal ball placement groove (10); a rotatable universal ball (11) is placed in the universal ball placement groove (10); a universal ball locking bolt (12) is provided on the universal ball placement groove (10); the universal ball (11) is fixedly connected to the motor fixing plate (15) via a connecting rod (13); a connecting rod placement groove (14) is provided on the universal ball placement groove (10) corresponding to the connecting rod (13); A motor (16) is fixed on the motor fixing plate (15), a light source placement turntable (17) is provided below the motor fixing plate (15), the motor (16) is capable of driving the light source placement turntable (17) to rotate, a plurality of excitation light sources are provided below the light source placement turntable (17), a sample placement table (8) is provided on the base (4) corresponding to the excitation light source, a quartz cup placement groove (81) is provided on the sample placement table (8), the sample placement table (8) and a height adjustment screw (9) are arranged in a protective cover (5), the protective cover (5) is placed on the base (4), and a top cover (6) is provided on the top of the protective cover (5).

2. The automatic excitation light source device according to claim 1, characterized in that: A total of six excitation light sources, namely, excitation light source one (181), excitation light source two (182), excitation light source three (183), excitation light source four (184), excitation light source five (185) and excitation light source six (186), are arranged at equal intervals below the light source placement turntable (17).

3. An automatic excitation light source device according to claim 2, characterized in that: The excitation light source one (181) is a white light source; the excitation light source two (182) is a purple light source; the excitation light source three (183) is a green light source; the excitation light source four (184) is a yellow light source; the excitation light source five (185) is a red light source; and the excitation light source six (186) is a blue light source.

4. The automatic excitation light source device according to claim 1, characterized in that: The protective cover (5) and the upper cover (6) are both made of light-proof materials.

5. The automatic excitation light source device according to claim 1, characterized in that: The middle part of the height adjustment screw rod (9) is hollow.

6. An automatic excitation light source device according to claim 1 or 4, characterized in that: The upper cover (6) is movably arranged on the top of the protective cover (5) via a hinge (61), and a handle (7) is provided above the upper cover (6).

7. The method for using the automatic excitation light source device according to claim 1, characterized in that The steps are: (1) Open the upper cover (6) on the top of the protective cover (5), and place the quartz cup containing the sample to be excited by the light source in the quartz cup placement groove (81) provided on the sample placement table (8); (2) Turn the height adjustment screw (9) to adjust the distance between the light source placement turntable (17) and the quartz cup; (3) Hold the connecting rod (13) and move it up and down to adjust the angle of the light source placement turntable (17) to align with the base (4), turn the universal ball locking bolt (12), and clamp the universal ball (11) into the universal ball placement groove (10); (4) Cover the top cover (6) of the protective cover (5); (5) Selecting the desired color of the excitation light source on the control computer (2), the controller (3) controls the motor (16) to drive the light source placement turntable (17) to rotate so that the excitation light source of the desired color is aligned with the quartz cup; (6) After setting the intensity parameters, excitation time parameters, sampling interval, excitation cycle and other parameters of the excitation light source on the control computer (2), the automatic excitation light source device can start working and complete the excitation irradiation of the sample by the excitation light source.

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