Mouse uterus fluorescence living body imaging fixing device

Abstract

The invention discloses a mouse uterus fluorescence living body imaging fixing device which comprises a base, an anesthesia module installed on the base is arranged on the base, a base inner bin is arranged in the inner space of the lower area of the base, an upper cover is connected with a uterus imaging module, and an installation assembly structure is arranged on the uterus imaging module; the upper cover enclosure is arranged at the edge of the upper cover, the upper cover and the upper cover enclosure are of an integrated structure, and an upper cover hollowed-out opening is formed in the upper portion of the upper cover; the constant-temperature heating plate is arranged in the base, and a fence integrated with the base is mounted at the edge in the base. The device is easy to operate, complex window suturing operation is not needed, an opening in a mouse abdominal operation is small, damage to the mouse is small, and the opening can be sutured to continue normal feeding after observation is completed; the uterus imaging module can effectively restrain shaking caused by heartbeat and breathing of a mouse, and a microscope can be conveniently used for observation.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, in particular to a mouse uterus fluorescent in vivo imaging fixing device. Background Art

[0002] Experimental animals should try to select animals whose functions, metabolism, structure and disease properties are similar to those of humans. Mice are the most commonly used in experiments, and mice are an important research object. Mice are small in size, convenient for experimental operation, and they are docile, easy to catch, and generally do not bite people, which is convenient for feeding and management; secondly, mice are very sensitive to external stimuli; thirdly, mice can provide animals of the same litter and different strains. Since animals are genetically uniform and individual differences are small, the experimental results are accurate and reliable. The body length of adult mice does not exceed 15.5 cm, with pointed faces and tentacles; ears stand upright and semicircular, eyes are large, noses are pointed, and tails are similar in length to the body. Observing the uterus of living mice using a fluorescence microscope is of great value for basic biological research and research on reproductive-related diseases. Direct observation based on the spontaneous fluorescence of uterine tissue can understand the structure and morphology of the uterus under physiological and pathological conditions; by fluorescently labeling specific tissues and cells of the uterus, the process and mechanism of the disease can be observed; by introducing fluorescently labeled drugs, the distribution, metabolism and efficacy of the drug in the uterus can be monitored.

[0003] At present, corresponding in vivo imaging strategies have been developed for different organs of mice. For example, a special skull fixation mechanism and brain window have been developed for the mouse brain. By tightly fastening the mouse skull, the interference of heartbeat and breathing can be better eliminated to achieve stable observation; for organs such as the liver, stomach, kidneys, spleen, and uterus in the mouse abdominal cavity, they can be observed by constructing a window in the abdominal opening. The classic strategy is: 1. Anesthetize the mouse and fix the limbs with the abdomen facing up, remove the abdominal hair and perform a surgical opening; 2. Suture a titanium alloy abdominal window with an imaging glass sheet along the opening, disinfect and rest after surgery; 3. Inject a fluorescent probe into the tail vein; 4. Anesthetize the mouse and fix it with the abdomen facing up, and observe through the abdominal window using a fluorescent microscope. The above-mentioned existing technologies can achieve fluorescent in vivo imaging of the mouse uterus to a certain extent, but there are still a series of problems that need to be improved: 1. The existing technology requires opening the mouse's abdomen and suturing the titanium alloy abdominal window, which is a cumbersome operation and poses a high risk of infection to the mouse after surgery. In addition, the abdominal window usually occupies more than 1 / 2 of the mouse's abdominal area, and long-term residence may cause pain and foreign body sensation in the mouse. 2. The uterus of mice is small (2 cm long and 2 mm in diameter) when not pregnant. After pregnancy, the uterus gradually increases in size. The existing titanium alloy abdominal window technology is more suitable for observing the uterus of mice that are pregnant for more than 9.5 days. For the uterus of mice that are not pregnant and in the early and middle stages of pregnancy (0.5-9.5 days), it is difficult to observe because it is small and easily blocked by other organs and tissues. 3. When using existing technology to perform fluorescent in vivo imaging of the mouse uterus, since the abdominal window is directly sutured to the abdomen, it is difficult to eliminate the jitter caused by the mouse's heartbeat and breathing, and the imaging picture is difficult to maintain stability, which is not conducive to fluorescent in vivo imaging that takes a long time (such as three-dimensional reconstruction of thick tissues and time-lapse imaging of specific locations); 4. The prior art usually fixes mice on a constant temperature heating plate and then directly places them under a microscope for observation. There is room for improvement in maintaining the anesthesia state of the mice, maintaining body temperature, and keeping the experimental environment clean. Summary of the invention

[0004] The purpose of the present invention is to provide a mouse uterus fluorescent in vivo imaging fixation device to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: A mouse uterus fluorescence in vivo imaging fixation device, comprising: A base, the base is provided with an anesthesia module installed thereon, the inner space of the lower area of ​​the base is set as a base inner compartment, an upper cover is provided at the upper end surface of the base, a uterine imaging module is connected to the upper cover, and a mounting component structure is provided on the uterine imaging module; An upper cover enclosure, wherein the upper cover enclosure is arranged at the edge of the upper cover, the upper cover and the upper cover enclosure are an integrated structure, and an upper cover hollow opening is opened above the upper cover; A constant temperature heating plate is arranged in a base, and an enclosure integral with the base is installed at the edge of the base.

[0006] Preferably, a charging indicator light, a battery charging port, a constant temperature heating switch, a temperature adjustment button and a temperature display screen are sequentially installed on the side of the base.

[0007] Preferably, a front enclosure opening and a rear enclosure opening are respectively provided in the middle of the front and rear ends of the enclosure.

[0008] Preferably, the anesthesia module comprises a head accommodating groove and a round hole holder, and the round hole holder is provided with a round bayonet.

[0009] Preferably, the inner compartment of the base is the internal space of the lower area of ​​the base, and the charging cable, power cable, control cable and lithium battery are distributed in the inner compartment of the base.

[0010] Preferably, the uterine imaging module includes a slide groove, a sliding block, a threaded hole and a rack. The slide groove is fixedly installed on the upper cover. A sliding block is arranged in the slide groove and is nested and slidable therewith. A threaded hole is opened on the upper surface of the sliding block, and a rack is arranged at the upper end of the side of the sliding block.

[0011] Preferably, a nut is provided in the threaded hole, and a butterfly piece is installed on the upper end of the nut.

[0012] Preferably, a control knob is fixedly mounted on the upper cover close to the rack, a screw is connected to the lower end of the control knob, and a gear is provided at the connection between the screw and the control knob, and the gear and the rack are meshed and connected with each other.

[0013] Preferably, a U-shaped titanium alloy thin rod is arranged between the nut and the upper surface of the sliding block, and the U-shaped titanium alloy thin rod is respectively provided with parallel front end and rear end handles.

[0014] Preferably, the U-shaped titanium alloy thin rod is connected to a receiving guide rail away from the nut, and the receiving guide rail is fixed to the upper cover.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The device of the present invention is easy to operate and can conveniently perform in vivo imaging of the mouse uterus without complicated window suturing operations. The surgical opening required for the mouse abdomen is small, which causes less harm to the mouse. After the observation is completed, the opening can be sutured and the normal feeding can be continued. The uterine imaging module of the present invention can effectively suppress the shaking caused by the heartbeat and breathing of the mouse, which is convenient for observation using a microscope; 2. The uterine imaging module in the present invention exposes the uterus in a semi-suspended manner, which can effectively reduce the interference caused by the mouse's breathing and heartbeat, and improve the stability of fluorescent live imaging. The device can be used to observe the state of the non-pregnant uterus of the mouse and the uterus during the entire pregnancy (0.5-20 days).

[0016] 3. The semi-enclosed structure of the present invention is conducive to maintaining the body temperature and anesthesia of mice, and is also conducive to keeping the experimental environment clean, avoiding damage or contamination of instruments due to leakage of mouse hair and body fluids. When the mouse is anesthetized by intraperitoneal injection of anesthetics, the fixing device has no external wiring, and the operation is more convenient when imaging with a microscope. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the base structure of the present invention.

[0018] Figure 2 It is a schematic diagram of the structure of the anesthesia module of the present invention.

[0019] Figure 3 It is a schematic diagram of the inner compartment structure of the base of the present invention.

[0020] Figure 4 It is a schematic diagram of the upper cover structure of the present invention.

[0021] Figure 5 It is a schematic diagram of the structure of the uterus imaging module of the present invention.

[0022] Figure 6 It is a schematic diagram of the installation structure of the U-shaped titanium alloy thin rod of the present invention.

[0023] Figure 7 It is a schematic diagram of the cross-sectional structure of the nut installation of the present invention.

[0024] Figure 8 It is a schematic diagram of the cross-sectional structure of the installation of the U-shaped titanium alloy thin rod of the present invention.

[0025] Fig. 9 It is a schematic diagram of the sliding block structure of the present invention.

[0026] Fig.10 This is a schematic diagram of the control knob structure of the present invention.

[0027] Fig.11 It is a schematic diagram of the installation structure of the nut and the sliding block of the present invention.

[0028] Fig.12 It is a schematic diagram of the side structure of the nut of the present invention.

[0029] Fig.13 This is a schematic diagram of the structure of the U-shaped titanium alloy thin rod of the present invention.

[0030] In the figure: 1, base; 101, enclosure; 102, constant temperature heating plate; 104, front enclosure opening; 105, charging indicator light; 106, battery charging port; 107, constant temperature heating switch; 108, temperature adjustment button; 109, temperature display screen; 110, rear enclosure opening; 2. Anesthesia module; 201. Head receiving slot; 202. Round hole card seat; 3. Inner compartment of the base; 301. Charging cable; 302. Power cable; 303. Control cable; 304. Lithium battery; 4. Upper cover; 401. Upper cover enclosure; 402. Hollow opening; 5. Uterine imaging module; 501. slide groove; 502. sliding block; 503. threaded hole; 504. rack; 6. Nut; 601. Butterfly piece; 7. Undertake the guide rail; 8. Control knob; 801. Screw rod; 802. Gear; 10. U-shaped titanium alloy thin rod; 1001. parallel front end; 1002. tail end handle. DETAILED DESCRIPTION

[0031] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0032] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0033] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0034] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] See also Figure 1-13 , an embodiment provided by the present invention: A mouse uterus fluorescent in vivo imaging fixing device comprises a base 1, an anesthesia module 2, a base inner compartment 3, an upper cover 4 and a uterus imaging module 5, wherein: A base 1, on which an anesthesia module 2 is installed, the inner space of the lower area of ​​the base 1 is set as a base inner compartment 3, an upper cover 4 is provided on the upper end surface of the base 1, a uterine imaging module 5 is connected to the upper cover 4, and a mounting component structure is provided on the uterine imaging module 5; An upper cover enclosure 401, which is arranged at the edge of the upper cover 4. The upper cover 4 and the upper cover enclosure 401 are an integrated structure. The upper cover 4 is provided with an upper cover hollow opening 402. The enclosure 401 can be covered with the base 1 to form a semi-enclosed heat preservation chamber. An upper cover hollow opening 402 is provided above the upper cover 4, which is just located above the abdomen of the anesthetized mouse on the constant temperature heating plate 102 of the base 1, so as to facilitate surgery and subsequent imaging of the mouse uterus. The left and right sides of the hollow opening 402 of the upper cover 4 are uterine imaging modules 5, which are used to expose and fix the mouse uterus during fluorescent in vivo imaging; A constant temperature heating plate 102 is arranged in the base 1, and an integral enclosure 101 is installed at the edge of the base 1. A front enclosure opening 104 and a rear enclosure opening 110 are respectively opened in the middle of the front and rear ends of the enclosure 101. The outlet of the anesthetic gas hose can enter and be limited through the front enclosure opening 104 on the base 1, and can be moved out of the mouse tail through the rear enclosure opening 110 for tail vein injection of drugs or fluorescent probes.

[0036] In this example, a charging indicator light 105, a battery charging port 106, a constant temperature heating switch 107, a temperature adjustment button 108 and a temperature display screen 109 are sequentially installed on the side of the base 1. The base inner compartment 3 is the internal space of the lower area of ​​the base 1. A charging line 301, a power line 302, a control line 303 and a lithium battery 304 are distributed in the base inner compartment 3. The constant temperature heating plate 102 takes the electric energy in the internal lithium battery through the power line 302. When the rechargeable battery is charged, the current passes through the charging line 301. The charging indicator light 105 is used to prompt the charging status. When not charging, there is no light. When the red light is on, it is charging. When the green light is on, it is fully charged. The constant temperature heating switch 107 can control the opening and closing of the constant temperature heating plate 102. The temperature adjustment button 108 can set the temperature of the constant temperature heating plate 102. The set temperature is displayed on the temperature display screen 109 as "SET + the set temperature value". When the temperature adjustment button 108 is not operated, the temperature display screen 109 is displayed as "RT + The temperature value" displays the current temperature. The buttons of the control panel and the constant temperature heating plate 102 are used to exchange commands and signals through the control line 303.

[0037] In this example, the anesthesia module 2 includes a head accommodating groove 201 and a round hole holder 202, and a round hole holder 202 is provided with a round bayonet. In one embodiment, the anesthesia module 2 is provided with a head accommodating groove 201 and a round hole holder 202, and the air outlet of the anesthetic gas hose enters through the front enclosure opening 104 on the base 1 and is fixed in the round bayonet on the round hole holder 202. The position of the air outlet of the anesthetic gas hose is adjusted to engage the mouth and nose of the mouse to maintain the anesthesia of the mouse.

[0038] Alternatively, if the mouse is anesthetized by intraperitoneal injection of anesthetic, the head of the mouse only needs to be fixed to the head receiving slot 201 after the mouse is anesthetized, without the need to connect an additional anesthetic gas hose. The benefit is that the entire fixing device can be free of any external wiring, which is conducive to improving the flexibility of microscope imaging.

[0039] In this example, the uterine imaging module 5 includes a slide groove 501, a sliding block 502, a threaded hole 503 and a rack 504. The slide groove 501 is fixedly installed on the upper cover 4. A sliding block 502 is arranged in the slide groove 501 to be nested and slid therewith. A threaded hole 503 is provided on the upper surface of the sliding block 502. A rack 504 is arranged at the upper end of the side of the sliding block 502. A nut 6 is arranged in the threaded hole 503, and a butterfly piece 601 is installed on the upper end of the nut 6. Screwing the butterfly piece 601 drives the nut 6 to rotate to achieve its tightening work.

[0040] In this example, the upper cover 4 is fixedly installed with a control knob 8 close to the rack 504, the lower end of the control knob 8 is connected to a screw 801, and a gear 802 is provided at the connection between the screw 801 and the control knob 8, the gear 802 and the rack 504 are meshed with each other, and the rotation of the control knob 8 can drive the sliding block 502 to move smoothly along the slide groove 501, and a U-shaped titanium alloy thin rod 10 is provided between the nut 6 and the upper surface of the sliding block 502, and the rear end of the U-shaped titanium alloy thin rod 10 is pushed and pulled to control its parallel front end to extend The length of the U-shaped titanium alloy thin rod 10 passes through the tissue below the mouse uterus and extends to the receiving guide rail 7 on the left side of the hollow opening 402 to expose and fix the mouse uterus. The U-shaped titanium alloy thin rod 10 is respectively provided with a parallel front end 1001 and a tail end handle 1002. The U-shaped titanium alloy thin rod 10 is made of titanium alloy and has good biocompatibility and corrosion resistance. The edges of the two parallel front ends 1001 are polished to be smooth, which helps to improve the smoothness of passing through the tissue below the uterus and reduce damage to the organ tissue. The tail end handle 1002 is frosted, which is conducive to the control operation of pushing and pulling when fixing the mouse uterus.

[0041] In this example, the U-shaped titanium alloy rod 10 is connected to a receiving guide rail 7 away from the nut 6, and the receiving guide rail 7 is fixed to the upper cover 4. When in use, loosen the butterfly piece 601 to lift the nut 6, insert the U-shaped titanium alloy rod 10 along both sides of the nut, tighten the butterfly piece 601 to lower the nut 6 and clamp the U-shaped titanium alloy rod 10 on the surface of the sliding block 502.

[0042] First, anesthetize the mouse and fix it on the base 1. After closing the upper cover 4, loosen the butterfly piece 601 to raise the nut 6, pull the tail end handle 1002 of the U-shaped titanium alloy rod 10 to make the parallel front end 1001 away from the hollow opening 402 of the upper cover 4, perform laparotomy on the mouse through the hollow opening 402 of the upper cover 4, use tweezers to gently pick up the mouse uterus from the opening, rotate the control knob 8 to drive the sliding block 502 to translate steadily, so that the nut 6 is aligned with the mouse uterus, push the tail end handle 1002 of the U-shaped titanium alloy rod 10, so that the parallel front end 1001 of the U-shaped titanium alloy rod 10 passes through the gap below the mouse uterus and reaches the receiving guide rail 7 on the left side of the hollow opening 402, tighten the butterfly piece 601 to lower the nut 6 and clamp the U-shaped titanium alloy rod 10 on the surface of the sliding block 502, thereby exposing and fixing the mouse uterus.

[0043] The working principle of the present invention is as follows: the experimental mouse is anesthetized in a gas anesthesia machine, and the abdomen is depilated with a depilatory cream. Then the constant temperature heating switch 107 of the base 1 is turned on, and the anesthetized mouse is placed on the constant temperature heating plate 102 of the base 1, with the abdomen facing upward, the limbs and the tail are fixed with medical tape, the head is fixed to the head receiving groove 201 of the anesthesia module 2, the gas outlet of the anesthetic gas hose is fixed to the circular bayonet in front of the circular hole holder 202, the position of the gas outlet is adjusted so that it just engages with the mouth and nose of the mouse, and the anesthetic gas is introduced to maintain the anesthesia of the mouse.

[0044] Alternatively, if the mouse is anesthetized by intraperitoneal injection of anesthetic, there is no need to introduce anesthetic gas, and the head of the anesthetized mouse can be directly fixed in the head receiving groove 201. The entire device has no external wiring, which can improve flexibility during in vivo imaging.

[0045] Optionally, if a tail vein injection of fluorescent dye or drug is required, the mouse tail can be removed through the rear enclosure opening 110, and after the injection, the mouse tail can be moved inside the enclosure and fixed again with medical tape.

[0046] Close the upper cover 4 to form a semi-enclosed space with the base 1, loosen the butterfly piece 601 and pull the tail handle 1002 of the U-shaped titanium alloy thin rod 10, so that the parallel front end 1001 is away from the hollow opening 402, use surgical scissors to make a 5 mm small incision on the left (right) side of the middle of the mouse's lower abdomen through the hollow opening 402 of the upper cover 4, use tweezers to clamp the uterus and gently lift it. Loosen the butterfly piece 601 on the sliding block 502, push the U-shaped titanium alloy thin rod 10 through the gap below the uterus, and the parallel front end 1001 of the thin rod reaches the receiving guide rail 7 on the left side of the hollow opening. Tighten the butterfly piece 601 to fix the U-shaped titanium alloy thin rod 10, that is, the mouse uterus is exposed and fixed at the same time. Place the entire in vivo imaging fixture with the mouse uterus on the microscope stage, rotate the control knob 8 to drive the U-shaped titanium alloy thin rod 10 on the sliding block 502 to translate steadily, thereby exposing different areas of the mouse uterus, adjusting the position of the mouse uterus to be observed, and using a microscope for fluorescent in vivo imaging.

[0047] After the in vivo imaging is completed, the entire uterus in vivo imaging fixture with the mouse is moved out of the microscope area and placed on the laboratory table. The butterfly piece 601 is loosened, and the U-shaped titanium alloy thin rod 10 is gently pulled out to break away from the contact with the uterus. The mouse uterus is stuffed back into the abdominal cavity with tweezers, and the abdominal wound of the mouse can be sutured to continue feeding. Finally, the constant temperature heating plate 102 is turned off and cleaned.

[0048] The above is only an embodiment of the present invention, and the common sense such as the known specific structure and characteristics in the scheme is not described in detail here. For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the attached claims rather than the above description, so it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

Claims

1. A mouse uterus fluorescence in vivo imaging fixation device, characterized in that: It includes: A base (1), an anesthesia module (2) being arranged on the base (1), the internal space of the lower region of the base (1) being set as a base inner chamber (3), an upper cover (4) being arranged on the upper end surface of the base (1), a uterine imaging module (5) being connected to the upper cover (4), and a mounting component structure being arranged on the uterine imaging module (5); An upper cover enclosure (401), the upper cover enclosure (401) being arranged at the edge of the upper cover (4), the upper cover (4) and the upper cover enclosure (401) being an integral structure, and an upper cover hollow opening (402) being provided above the upper cover (4); A constant temperature heating plate (102), wherein the constant temperature heating plate (102) is arranged in the base (1), and an enclosure (101) integral with the base (1) is installed at the edge of the base (1).

2. A mouse uterus fluorescence in vivo imaging fixation device according to claim 1, characterized in that: A charging indicator light (105), a battery charging port (106), a constant temperature heating switch (107), a temperature adjustment button (108) and a temperature display screen (109) are sequentially mounted on the side of the base (1).

3. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: A front enclosure opening (104) and a rear enclosure opening (110) are respectively provided in the middle of the front and rear ends of the enclosure (101).

4. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: The anesthesia module (2) comprises a head receiving groove (201) and a round hole holder (202), and a round socket is provided on the round hole holder (202).

5. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: The base inner compartment (3) is the internal space of the lower area of ​​the base (1), and a charging line (301), a power line (302), a control line (303) and a lithium battery (304) are distributed in the base inner compartment (3).

6. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: The uterine imaging module (5) comprises a slide groove (501), a sliding block (502), a threaded hole (503) and a rack (504); the slide groove (501) is fixedly mounted on the upper cover (4); a sliding block (502) is arranged in the slide groove (501) and is nested and slidable therewith; a threaded hole (503) is provided on the upper surface of the sliding block (502); and a rack (504) is arranged at the upper end of the side of the sliding block (502).

7. The mouse uterus fluorescent in vivo imaging fixation device according to claim 6, characterized in that: A nut (6) is disposed in the threaded hole (503), and a butterfly piece (601) is mounted on the upper end of the nut (6).

8. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: A control knob (8) is fixedly mounted on the upper cover (4) close to the rack (504); a screw rod (801) is connected to the lower end of the control knob (8); a gear (802) is provided at the connection between the screw rod (801) and the control knob (8); the gear (802) and the rack (504) are meshedly connected to each other.

9. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: A U-shaped titanium alloy thin rod (10) is arranged between the nut (6) and the upper surface of the sliding block (502), and a parallel front end (1001) and a rear end handle (1002) are respectively arranged on the U-shaped titanium alloy thin rod (10).

10. The mouse uterus fluorescent in vivo imaging fixation device according to claim 1, characterized in that: The U-shaped titanium alloy thin rod (10) is connected to a receiving guide rail (7) at a position away from the nut (6), and the receiving guide rail (7) is fixed to the upper cover (4).

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