Chinese herbal medicine mask for relieving chloasma

Abstract

The invention discloses a Chinese herbal medicine mask for relieving chloasma, and belongs to the field of skin care products. Comprising a safflower flower extract, liquorice, medicinal rhubarb root, ginseng, a cordyceps militaris extract and a rhizoma smilacis glabrae root extract. By compounding various components, the anti-inflammatory, anti-oxidation and antibacterial effects are achieved, so that the effect of improving chloasma is achieved; meanwhile, after compounding, a synergistic interaction effect is achieved.

Description

Technical Field

[0001] The invention belongs to the field of skin care products, and particularly relates to a Chinese herbal medicine facial mask for alleviating chloasma. Background Art

[0002] Melasma is a common hyperpigmented skin disease, also known as butterfly spots and liver spots, which is common in young and middle-aged women, especially after pregnancy and women of childbearing age. The course of the disease is slow, without other discomfort, and it is often symmetrically distributed, dark brown or light brown patches, distributed on the cheeks, forehead, nose, lips, around the eyes, etc. Chinese medicine believes that melasma is related to liver congestion, so it is also called liver spots.

[0003] Melasma has a variety of causes; common external causes include:

[0004] Ultraviolet rays: can stimulate melanocytes to produce and transport melanin, leading to pigmentation.

[0005] Impaired skin barrier function: may lead to enhanced melanin synthesis and obstructed transport.

[0006] An imbalance of microorganisms on the skin, particularly an increase in the number of pigment-producing Micrococci.

[0007] Oxidative stress: An increase in free radicals may lead to the accumulation of pigments and the formation of melasma.

[0008] Although laser instruments and chemically synthesized functional skin care products are widely available in the market, skin irritation, skin peeling and uncontrollable phototherapy frequency caused by product application can directly lead to weakening, damage and sensitivity of the skin's secondary stratum corneum. It has always been difficult for people to find a gentle and non-irritating way to fully reduce facial spots without any risk of laser instruments and chemical skin care products causing secondary skin damage.

[0009] Therefore, there is a need to develop a milder external skin composition for melasma. Summary of the invention

[0010] The invention provides a Chinese herbal medicine facial mask for alleviating chloasma, which achieves the purpose of alleviating chloasma by compounding a plurality of Chinese herbal medicine components.

[0011] In order to achieve the above object, the present invention adopts the following technical solutions:

[0012] A Chinese herbal medicine composition for alleviating chloasma comprises: safflower flower extract, liquorice, medicinal rhubarb root, ginseng, cordyceps militaris extract, and smilax glabra root extract.

[0013] The following components are included by mass:

[0014]

[0015] Preferably, the licorice is licorice root extract.

[0016] Preferably, the ginseng is ginseng leaf / stem extract.

[0017] Preferably, it further comprises Forsythia suspensa, wherein the Forsythia suspensa is Hypericum perforatum oil; and the added amount of the Forsythia suspensa is 60-80 parts by weight.

[0018] A skin external preparation, comprising the aforementioned Chinese herbal medicine composition for alleviating chloasma.

[0019] Preferably, the addition ratio of the Chinese herbal composition for alleviating chloasma is 5-20wt%.

[0020] Preferably, the leave-on skin topical preparation further comprises an auxiliary material, wherein the auxiliary material is selected from one or more of a thickener, an emulsifier, a skin conditioner, a moisturizer, a pH adjuster and a preservative.

[0021] Preferably, the dosage form of the leave-on skin topical preparation is a facial mask.

[0022] The mechanism of reducing chloasma in the present invention is mainly divided into the following aspects:

[0023] Chinese medicine pharmacology:

[0024] Licorice: mild in nature, sweet in taste, moisturizing and anti-inflammatory.

[0025] Medicinal rhubarb root: cools blood, detoxifies, removes blood stasis and promotes menstruation.

[0026] Wild Smilax glabra: sweet and light in taste, neutral in nature, can remove spots and whiten skin.

[0027] Safflower has the effect of invigorating blood circulation and promoting menstruation.

[0028] Cordyceps militaris: replenishes qi and blood, nourishes the liver and kidneys.

[0029] Active ingredients:

[0030] Safflower: It is rich in safflower flavonoids (such as myricetin and kaempferol), which have anti-inflammatory and antioxidant effects and play a positive role in the treatment of melasma. It should be noted that safflower and saffron are not the same plant. The present study found that the effect of saffron is far less than that of safflower, so it needs to be carefully distinguished.

[0031] Licorice: contains glycyrrhizin, glycyrrhizic acid, glycyrrhizin, glycyrrhizin flavonoids and other ingredients, has good anti-inflammatory and anti-allergic effects, can help relieve skin inflammation, and reduce the probability of chloasma. At the same time, glycyrrhizic acid also has a whitening effect, can inhibit the formation of melanin, and help alleviate chloasma. The present invention found that although the whitening effect of Glycyrrhiza glabra is better, the anti-inflammatory and anti-allergic effects of licorice are more superior than those of Glycyrrhiza glabra.

[0032] Medicinal rhubarb: contains chrysophanol and rhubarb, which have good antioxidant and anti-inflammatory effects, can help relieve skin inflammation and reduce the incidence of chloasma. Rhubarb is divided into medicinal rhubarb, palm leaf rhubarb, wavy leaf rhubarb, tangut rhubarb and other different species of plants, which should be carefully distinguished.

[0033] Ginseng: Ginseng is rich in ginsenosides. However, the ginsenoside content of the most commonly eaten part, ginseng root, is lower than that of the stem / leaf. The present invention uses ginseng leaf / stem extract to obtain a higher content of ginsenosides.

[0034] Cordyceps militaris: It has multiple effects such as anti-inflammatory, antibacterial, and antioxidant. The cordycepin in it has a good inhibitory effect on microorganisms such as streptococci, and can help reduce skin inflammation. At the same time, the cordyceps polysaccharides, polyphenols, flavonoids and other substances in Cordyceps militaris have strong antioxidant capacity, which can remove free radicals, protect skin cells from oxidative damage, and thus improve chloasma.

[0035] Smilax glabra: Poria cocos polysaccharide has antioxidant and anti-inflammatory properties, thus helping to reduce melasma.

[0036] Hypericum perforatum oil: has anti-inflammatory, antibacterial and antioxidant properties, can help reduce skin inflammation, and also has certain moisturizing properties.

[0037] Additionally, Hypericum perforatum oil is rich in antioxidants, which help fight free radical damage and slow the aging process of the skin.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] The invention achieves anti-inflammatory, anti-oxidative and antibacterial effects by compounding a variety of ingredients, thereby improving chloasma; and at the same time, the compounding has a synergistic effect. DETAILED DESCRIPTION

[0040] In order to better understand the present invention, the present invention is further described below in conjunction with specific examples, wherein the terms used in the examples are for describing specific embodiments and do not constitute limitations on the scope of protection of the present invention.

[0041] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or under conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are calculated by weight.

[0042] The sources of some raw materials involved in the present invention are shown in Table 1 below.

[0043] Table 1 Sources of some raw materials

[0044]

[0045]

[0046] Preparation method of Chinese herbal medicine composition for alleviating chloasma:

[0047] (1) Take Hypericum perforatum oil and deionized water, add emulsifier (fatty alcohol polyoxyethylene ether), and pre-stir to form an emulsion;

[0048] (2) Keep stirring and add the remaining ingredients to the emulsion; continue stirring until it becomes a uniform paste, and store and seal. The specific amounts used in each embodiment and comparative example are shown in Table 2 below.

[0049] Table 2 Oil control composition ingredients

[0050]

[0051]

[0052] Note: The units in the table are parts by mass, and “-” means no corresponding addition.

[0053] Compared with Example 2, Comparative Example 4 lacks safflower, which is supplemented by an equal amount of ginseng.

[0054] Compared with Example 2, Comparative Example 5 lacks rhubarb, which is supplemented by an equal amount of ginseng.

[0055] Compared with Example 2, Comparative Example 6 lacks Hypericum perforatum oil, which is supplemented by an equal amount of ginseng. At the same time, since the remaining ingredients are all water-soluble, no emulsifier is added.

[0056] Comparative Example 7 is compared with Example 2, in which an equal amount of safflower is replaced by saffron.

[0057] Comparative Example 8 Compared with Example 2, an equal amount of liquorice was replaced by Glycyrrhiza glabra.

[0058] Comparative Example 9 Compared with Example 2, an equal amount of Hypericum perforatum oil was replaced with Forsythia suspensa extract, and since the remaining ingredients were all water-soluble, no emulsifier was added.

[0059] Comparative Example 10 Compared with Example 2, ginseng roots were used to replace the same amount of ginseng leaves / stems.

[0060] Compared with Example 2, in Comparative Example 11, only ginseng leaf / stem extract was added, and the rest was supplemented with deionized water.

[0061] Compared with Example 2, Comparative Example 12 only added liquorice root extract, and the rest was supplemented with deionized water.

[0062] In Comparative Example 13, compared with Example 2, only safflower flower extract was added, and the rest was supplemented with deionized water.

[0063] Compared with Example 2, Comparative Example 14 only adds Hypericum perforatum oil, and the rest is deionized water, wherein the emulsifier has an emulsifying and dispersing effect.

[0064] Performance Testing

[0065] 1. Safety Testing

[0066] With reference to the 2015 Technical Specifications for Safety of Cosmetics, the samples prepared in the embodiments and comparative examples were evaluated for cosmetic irritation. The test method was a skin patch test, and 30 randomly distributed people aged 16-65 years were tested.

[0067] Test method:

[0068] The sample was diluted to 50% concentration (active ingredient concentration 25%) by adding 1-fold deionized water.

[0069] Place the test substance in a patch tester at a dosage of 0.020-0.025g, and cover the patch tester with the test substance on the back or forearm of the subject with a non-irritating cloth-based tape, and gently press it with the palm of your hand to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction 30 minutes after the indentation disappears. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0070] Evaluation criteria:

[0071] Grade 0: negative reaction;

[0072] Grade 1: Suspicious reaction, only slight erythema;

[0073] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0074] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0075] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and the reaction exceeds the test area.

[0076] Test results: Examples 1-3, Comparative Examples 4-8, 10-14 all showed negative skin reactions; Comparative Example 9 showed grade 1 and grade 2 reactions.

[0077] 2. Antioxidant Test

[0078] The antioxidant capacity of the comparative examples was verified using DPPH free radicals.

[0079] Prepare a DPPH solution with a concentration of 0.1 mmol / L and take the diluted Example and Comparative Example;

[0080] Prepare 10g / L sample solution, positive control Vc solution and blank control aqueous solution. Take 0.1ml sample solution and 4.9ml DPPH solution respectively and place them in a dark room for reaction for 20 minutes, then measure the absorbance at 517nm wavelength and calculate the clearance rate DVC%.

[0081]

[0082] In the examples and comparative examples, 19 times of deionized water was added to dilute the samples to 5% concentration. The results are shown in Table 2.

[0083] Table 2 DPPH free radical scavenging rate

[0084]

[0085]

[0086] The results in Table 2 show that:

[0087] Comparing the results of Example 2 with Comparative Examples 4-6, it can be seen that the combination of different components can produce a synergistic effect on the antioxidant effect, and the antioxidant effect achieved by the present invention cannot be achieved if any one of the components is omitted.

[0088] Comparing the results of Example 2 with Comparative Examples 7-10, it can be seen that the composition of different components can produce a synergistic effect on the antioxidant effect, and changing any one of them cannot achieve the antioxidant effect achieved by the present invention.

[0089] The results of Comparative Examples 11-14 show that the antioxidant performance of a single component does not meet the requirements and is relatively weaker than that of the composite component.

[0090] 3. Inflammatory Factor Inhibition Test

[0091] TNF-α is the most common inflammatory factor in inflammatory response; the anti-inflammatory effect of the ingredient can be tested by inhibiting TNF-α.

[0092] RAW264.7 cells grown to the logarithmic phase were cultured at 1.5×10 5Cells were inoculated at a density of 100 μg / mL in a 24-well plate, with 800 μL of cell suspension per well, and cultured in a 37°C, 5% CO2 incubator for 24 hours. When the cells grew to near fusion, 50 μL of the sample concentration of 10 mg / L was administered, with 3 replicates per group. The positive control was 50 μL of 10 μg / mL dexamethasone, and 1 μg / mL lipopolysaccharide (LPS) was used for stimulation 1 hour later. After incubation in a cell culture incubator for 24 hours, the cell culture fluid was transferred to a centrifuge tube, centrifuged at 5000 r / min for 10 minutes, and the cell supernatant was collected and tested according to the instructions of the mouse TNF-α enzyme-linked immunosorbent assay kit.

[0093]

[0094] 19 times of deionized water was added to dilute the samples to 5% concentration in the examples and comparative examples. The results are shown in Table 3.

[0095] Table 3 Results of TNF-α secretion by RAW

[0096]

[0097]

[0098] IV. Human efficacy test

[0099] The composition of the above-mentioned Example 2 was prepared into a mud mask, and the specific formulation was as follows:

[0100]

[0101] Preparation: Mix the above ingredients evenly and stir until it becomes a puree without any grains.

[0102] Test method: After cleansing the face, apply an appropriate amount evenly on the face, then wash off with clean water after 15 minutes, once every 2 days.

[0103] Eight volunteers were recruited, all of whom were patients with mild to moderate melasma. The test lasted for 30 days; follow-up visits were conducted after 30 days.

[0104] All 8 volunteers said that the mask did not cause any allergic symptoms such as redness, swelling, and itching, and that it helped alleviate chloasma, making the skin tone more even and fair.

[0105] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. A Chinese herbal medicine composition for alleviating chloasma, characterized in that: include: Carthamus tinctorius flower extract, licorice root, rhubarb root, ginseng, cordyceps militaris extract, smilax glabra root extract.

2. The Chinese herbal medicine composition for alleviating chloasma according to claim 1, characterized in that: The following components are included by mass:

3. The Chinese herbal medicine composition for alleviating chloasma according to claim 1, characterized in that: The licorice is licorice root extract; and the ginseng is ginseng leaf / stem extract.

4. The Chinese medicinal composition for alleviating chloasma according to claim 1, characterized in that: Also includes Forsythia suspensa.

5. The Chinese herbal medicine composition for alleviating chloasma according to claim 4, characterized in that: The forsythia suspensa is Hypericum perforatum oil.

6. The Chinese herbal medicine composition for alleviating chloasma according to claim 4, characterized in that: The added amount of the Forsythia suspensa is 60-80 parts by weight.

7. A skin external preparation, characterized in that: A Chinese herbal medicine composition for alleviating chloasma comprising any one of claims 1-6.

8. The leave-on skin external preparation according to claim 7, characterized in that: The addition ratio of the Chinese herbal medicine composition for alleviating chloasma is 5-20wt%.

9. The leave-on skin external preparation according to claim 7, characterized in that: The composition further comprises auxiliary materials, which are selected from one or more of thickeners, emulsifiers, skin conditioners, moisturizers, pH regulators and preservatives.

10. The leave-on skin external preparation according to claim 7, characterized in that: The dosage form is a facial mask.

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