Treatment method and application of epimedium seeds

Abstract

The invention provides a treatment method and application of epimedium seeds, and belongs to the technical field of traditional Chinese medicinal material planting. Comprising the following steps: S1, mixing herba epimedii seeds with sand, adding a bactericide solution, and stirring to obtain sand-stored herba epimedii seeds; s2, bagging the sand-stored herba epimedii seeds obtained in the step S1, storing the sand-stored herba epimedii seeds at 15 DEG C for 14 days, performing magnetic field treatment on the sand-stored herba epimedii seeds every day in the last 5-9 days within the 14 days, lowering the storage temperature to 13 DEG C, and performing gradient cooling in a manner of lowering the storage temperature to 2 DEG C every two weeks until the storage temperature is lowered to 5 DEG C to obtain low-temperature herba epimedii seeds; s3, storing the low-temperature herba epimedii seeds obtained in the step S2 at constant temperature and constant humidity, performing magnetic field treatment on the low-temperature herba epimedii seeds once every 2-4 days of storage, supplementing water, and sowing after 70-80 days of storage. According to the method, the dormancy period of the epimedium seeds can be effectively shortened, the germination speed is increased, and the budding rate is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese medicinal material planting and relates to a processing method and application of epimedium seeds. Background Art

[0002] Epimedium, also known as Gangqian, Xianlingpi, Sanzhi Jiuyecao, Ganxiongjin, and Yanghuo, is an important Chinese medicinal material with the effects of nourishing the kidney and strengthening yang, strengthening tendons and bones, and removing wind and dampness. Epimedium brevicornum Maxim) is a perennial herb, 30-40cm high, with a horizontal rhizome, hard texture, and many fibrous roots. It grows in the shade and wet places of hillsides or under forests in valleys. The flowering period is May-June, and the fruiting period is June-August. The follicles are 1cm long, with a beak at the tip, and contain 1-2 seeds, which are brown. Epimedium seeds have a unique physiological dormancy period. After maturity, they are easy to fall off and the seedling rate is low, resulting in low reproduction efficiency of Epimedium, which limits the artificial large-scale cultivation of Epimedium.

[0003] Han Wei et al. published in the article "Effects of Different Treatments on the Dormancy Breaking of Epimedium Seeds" (Shaanxi Agricultural Science, 2020, No. 2, pp. 50-52, 3 pages in total) that the treatment of epimedium seeds by sand storage and gibberellin soaking can help improve the germination rate of epimedium seeds and shorten the germination time of epimedium. They also pointed out that low-temperature sand storage, seed pod removal and a certain concentration of gibberellin treatment are necessary for the germination of epimedium seeds.

[0004] Chinese invention patent CN116897776A provides a storage method, a cultivation method and a planting method for epimedium seeds. The epimedium seed storage method provided by this technology includes the following steps: mixing sand, epimedium seeds, water and fungicide, and then refrigerating. The initial refrigeration temperature is 15-16°C, and the temperature is lowered by 2-3°C every two weeks to a refrigeration temperature of 4-5°C and then maintained constant; after maintaining a constant temperature, the humidity is adjusted to 65%-67% for continuous refrigeration. The cultivation method of epimedium seeds includes the following steps: sowing the epimedium seeds treated by the above storage method on a sand bed, maintaining the humidity of the sand bed at 65%-67%, and providing 16-17h·d of light. -1 This storage method can effectively shorten the germination time and increase the germination rate.

[0005] Chinese invention patent CN114946318A provides a method for promoting the germination of seeds of Epimedium. The method comprises the following steps: stratifying the seeds of Epimedium at 10°C for 30-60 days under dark conditions and then germinating them at 4°C. This technology has found that the seeds of Epimedium have the characteristics of morphological and physiological dormancy. The morphological dormancy can be quickly released by stratification at 10°C, and the physiological dormancy can be released by stratification at 4°C, which solves the key technology of seed germination in the industrial cultivation process of Epimedium. The experimental results show that the sterilization method, stratification and germination technology of this technology significantly increase the germination rate, germination rate and seedling rate of seeds, greatly improving the economic benefits.

[0006] Modern research shows that magnetic fields have a certain effect on the germination process of seeds. Using magnetic fields to treat seeds has the advantages of being non-invasive and easy to adjust the intensity, and can regulate the germination behavior of seeds by regulating the behavior of small molecules and / or biomacromolecules with different charges inside the seeds. The magnetic field can affect physiological activities and parameters such as the water absorption process, respiration, and plant hormone levels inside the seeds. However, the appropriate magnetic field intensity and frequency for promoting germination of seeds of different plants are different. The prior art has not yet provided a magnetic field-assisted epimedium seed treatment method to further improve the germination rate of epimedium seeds and shorten the germination time. Summary of the invention

[0007] In view of this, in view of the above problems in the prior art, the object of the present invention is to provide a method for processing epimedium seeds and application thereof.

[0008] To achieve the above object of the invention, on the one hand, the present invention provides a method for treating epimedium seeds, comprising the following steps: S1. Mixing epimedium seeds with sand, adding a fungicide solution, and stirring to obtain sand-hidden epimedium seeds; S2, bagging the epimedium seeds obtained in step S1, storing them at 15° C. for 14 days, subjecting the epimedium seeds to magnetic field treatment every day for the last 5-9 days within the 14 days of storage, lowering the storage temperature to 13° C., and gradually cooling the storage temperature by lowering it by 2° C. every two weeks until the storage temperature drops to 5° C., thereby obtaining low-temperature epimedium seeds; S3, storing the low-temperature epimedium seeds obtained in step S2 at a constant temperature and humidity, subjecting the low-temperature epimedium seeds to magnetic field treatment once every 2-4 days of storage, replenishing water, and storing for 70-80 days to obtain epimedium seeds to be sown.

[0009] Preferably, in step S1, the epimedium seeds are pretreated.

[0010] Preferably, in step S1, the volume ratio of the epimedium seeds to the sand is 19-21:1.

[0011] Preferably, in step S2, the duration of the magnetic field treatment is 10-50 min, and the magnetic field intensity of the magnetic field treatment is 17.6-24.5 mT.

[0012] More preferably, in step S2, the duration of the magnetic field treatment is 30 min, and the magnetic field intensity of the magnetic field treatment is 21.0 mT.

[0013] Preferably, in step S2, the Herba Epimedii seeds are subjected to magnetic field treatment every day in the last 7 days of the storage within 14 days.

[0014] Preferably, in step S3, the duration of the magnetic field treatment is 10-50 min, and the magnetic field intensity of the magnetic field treatment is 5.3-12.5 mT.

[0015] More preferably, in step S3, the duration of the magnetic field treatment is 30 min, and the magnetic field intensity of the magnetic field treatment is 8.9 mT.

[0016] Preferably, in step S3, the low-temperature epimedium seeds are treated with a magnetic field once every 3 days of storage.

[0017] On the other hand, the present invention provides application of the above-mentioned treatment method in epimedium cultivation.

[0018] Compared with the prior art, the present invention has the following beneficial effects: The present invention uses a Helmholtz coil to generate a constant magnetic field of a specific intensity to perform magnetic field treatment on epimedium seeds during cold storage and sand storage, thereby further shortening the dormancy time of the epimedium seeds, improving the germination rate of the epimedium seeds planted in sand tables and fields, shortening the germination time, and being beneficial to the development of large-scale planting of epimedium. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 1 is a schematic diagram of the structure of a Helmholtz coil magnetic field generator used for magnetic treatment of Epimedium in various embodiments and comparative examples; wherein 1 and 2 represent Helmholtz coils, 3 represents a coil support, 4 represents a horizontal tray, and 5 represents a tray support.

[0020] Figure 2 This is a step diagram of the process of treating Epimedium seeds with low temperature and magnetic field in step S2 of Example 1.

[0021] Figure 3 This is a step diagram of the process of treating Epimedium seeds with low temperature and magnetic field in step S2 of Example 10.

[0022] Figure 4 This is a step diagram of the process of treating Epimedium seeds with low temperature and magnetic field in step S2 of Example 11.

[0023] Figure 5 This is a step diagram of the process of treating Epimedium seeds with low temperature and magnetic field in step S2 of Comparative Example 6. DETAILED DESCRIPTION

[0024] Terms and Claims of the Present Invention: 1. The articles “a”, “an” and “the” include plural referents unless expressly limited to one or more referents otherwise.

[0025] 2. Numerical range: Unless otherwise expressly stated, all ranges or ratios disclosed herein will be understood to include any and all sub-ranges or sub-ratios contained therein. For example, a range or ratio of 1 to 30 stated should be considered to be included between a minimum value of 1 and a maximum value of 30, and any sub-range or sub-ratio, integer, decimal, or sub-range or sub-ratio consisting of integers or decimals including the end points.

[0026] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0027] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Unless otherwise specified, the contents described below are all weight contents. Unless otherwise specified, it is understood that the process is carried out at room temperature.

[0028] In the following examples and comparative examples, the Helmholtz coil magnetic field generator used was purchased from Mianyang Litian Magnetic Technology Co., Ltd., model PEM-110XQ2.

[0029] Example 1 A method for processing epimedium seeds comprises the following steps.

[0030] S1. The epimedium seeds collected in June are washed, peeled, and naturally air-dried to form dried epimedium seeds. The dried epimedium seeds are pre-treated by washing, drying, soaking in warm water, and gently scrubbing for later use.

[0031] Mix epimedium seeds and sand in a volume ratio of 20:1 to obtain a dry mixture. Dilute 30% difenoconazole suspension of fungicide 2000 times with water and mix well to obtain a fungicide aqueous solution. Spray the obtained fungicide aqueous solution on the dry mixture continuously and stir evenly until it can be pinched into a sand ball by hand and the sand ball is placed on the table without liquid flowing out, thereby obtaining sand-hidden epimedium seeds.

[0032] S2, bag the epimedium seeds obtained in step S1, store them at 15°C for 7 days, take them out, spread them on a culture dish with a thickness of 1 cm, and place them on a tray at the center of the Helmholtz coil magnetic field generator. The positional relationship between the Helmholtz coil magnetic field generator and the tray is as follows: Figure 1 As shown. Turn on the magnetic field generator and adjust the current to 12A. At this time, the magnetic field strength is 21.0mT. The ambient temperature is maintained at 15°C during the magnetic field treatment. After 30 minutes of magnetic field treatment, the Epimedium seeds are re-packed. Carry out magnetic field treatment once a day, repeat for 7 consecutive days, and then lower the storage temperature to 13°C. Thereafter, the storage temperature is gradually lowered by lowering it by 2°C every two weeks, and it will no longer be lowered after reaching 5°C. The schematic diagram of the storage temperature and magnetic field treatment method is shown in Figure 2 As shown, low-temperature Epimedium seeds were obtained.

[0033] S3. Store the low-temperature epimedium seeds obtained in step S2 in a constant temperature and humidity storage cabinet. The temperature in the cabinet is 5°C and the humidity is 66%. Take out every 3 days of storage, spread it on a culture dish with a thickness of 1cm, and place it on a tray at the center of the Helmholtz coil magnetic field generator. Turn on the magnetic field generator and adjust the current to 5A. At this time, the magnetic field strength is 8.9mT. Maintain the ambient temperature of 5°C during the magnetic field treatment. After 30 minutes of magnetic field treatment, spray water into the tray, stir it, re-bag it, and put it back in the storage cabinet. After 75 days of storage, the epimedium seeds are ready for sowing.

[0034] Example 2 Compared with Example 1, the difference is that in step S2, the time of each magnetic field treatment is adjusted from 30 minutes to 50 minutes, and the rest are the same.

[0035] Example 3 Compared with Example 1, the difference is that in step S2, the time of each magnetic field treatment is adjusted from 30 minutes to 10 minutes, and the rest are the same.

[0036] Example 4 Compared with Example 1, the difference is that in step S2, the current of the magnetic field generator is adjusted to 14A, so that the magnetic field strength is adjusted to 24.5mT, and the rest are the same.

[0037] Example 5 Compared with Example 1, the difference is that in step S2, the current of the magnetic field generator is adjusted to 10A, so that the magnetic field strength is adjusted to 17.6mT, and the rest are the same.

[0038] Example 6 Compared with Example 1, the difference is that in step S3, the time of each magnetic field treatment is adjusted from 30 minutes to 50 minutes, and the rest are the same.

[0039] Example 7 Compared with Example 1, the difference is that in step S3, the time of each magnetic field treatment is adjusted from 30 minutes to 10 minutes, and the rest are the same.

[0040] Example 8 Compared with Example 1, the difference is that in step S3, the current of the magnetic field generator is adjusted to 3A, so that the magnetic field strength is adjusted to 5.3mT, and the rest are the same.

[0041] Example 9 Compared with Example 1, the difference is that in step S3, the current of the magnetic field generator is adjusted to 7A, so that the magnetic field strength is adjusted to 12.5mT, and the rest are the same.

[0042] Example 10 Compared with Example 1, the difference is that in step S2, the temperature of the sample was changed from 7 days at 15°C to 5 days at 15°C, and the magnetic field treatment was changed from 7 days to 9 days. The rest is the same. The schematic diagram of the storage temperature and magnetic field treatment method of step S2 of Example 10 is shown in FIG. Figure 3 shown.

[0043] Embodiment 11 Compared with Example 1, the difference is that in step S2, the temperature of the sample was changed from 7 days at 15°C to 9 days at 15°C, and the magnetic field treatment was changed from 7 days to 5 days. The rest is the same. The schematic diagram of the storage temperature and magnetic field treatment method of step S2 of Example 11 is shown in FIG. Figure 4 shown.

[0044] Example 12 Compared with Example 1, the difference is that in step S3, the magnetic field treatment is changed from being taken out every 3 days of storage to being taken out every 4 days of storage, and the rest are the same.

[0045] Example 13 Compared with Example 1, the difference is that in step S3, the magnetic field treatment is changed from being taken out every 3 days of storage to being taken out every 2 days of storage, and the rest are the same.

[0046] Embodiment 14 Compared with Example 1, the difference is that in step S2, the time of each magnetic field treatment is adjusted from 30 minutes to 60 minutes, and the rest are the same.

[0047] Embodiment 15 Compared with Example 1, the difference is that in step S3, the time of each magnetic field treatment is adjusted from 30 minutes to 60 minutes, and the rest are the same.

[0048] Example 16 Compared with Example 1, the difference is that in step S2, the current of the magnetic field generator is adjusted to 15A, so that the magnetic field strength is adjusted to 26.3mT, and the rest are the same.

[0049] Embodiment 17 Compared with Example 1, the difference is that in step S3, the current of the magnetic field generator is adjusted to 8A, so that the magnetic field strength is adjusted to 14.2mT, and the rest are the same.

[0050] Comparative Example 1 Compared with Example 1, the difference is that step S2 is replaced by the following: The sand-stored epimedium seeds obtained in step S1 are bagged and stored at 15° C. for 2 weeks. Thereafter, the temperature is gradually lowered by lowering the storage temperature by 2° C. every two weeks, and the temperature is no longer lowered after reaching 5° C., thereby obtaining low-temperature epimedium seeds.

[0051] The rest are the same.

[0052] Comparative Example 2 Compared with Example 1, the difference is that step S3 is replaced by the following: The low-temperature epimedium seeds obtained in step S2 are stored in a constant temperature and humidity storage cabinet. The temperature in the cabinet is 5°C and the humidity is 66%. After every 3 days of storage, the seeds are taken out, sprayed with water to maintain the humidity, stirred, re-packed, and put back into the storage cabinet. After 75 days of storage, the epimedium seeds are ready for sowing.

[0053] Comparative Example 3 Compared with Example 1, the difference is that step S2 is replaced by the following: bagging the sand-stored epimedium seeds obtained in step S1 and storing them at 15°C for 2 weeks. Thereafter, gradually cooling the temperature by lowering the storage temperature by 2°C every two weeks, and no longer lowering the temperature after reaching 5°C, thereby obtaining low-temperature epimedium seeds; At the same time, step S3 is replaced by the following: the low-temperature epimedium seeds obtained in step S2 are stored in a constant temperature and humidity storage cabinet. The temperature in the cabinet is 5°C and the humidity is 66%. After every 3 days of storage, the seeds are taken out, sprayed with water to maintain the humidity, stirred, re-packed, and put back into the storage cabinet. After 75 days of storage, the epimedium seeds are ready for sowing.

[0054] The rest are the same.

[0055] Comparative Example 4 Compared with Example 1, the difference is that in step S2, the storage at 15° C. for 7 days is changed to the storage at 15° C. for 2 days, and the magnetic field treatment is repeated for 12 days instead of 7 days. The rest is the same.

[0056] Comparative Example 5 Compared with Example 1, the difference is that in step S2, the storage at 15° C. for 7 days is changed to the storage at 15° C. for 12 days, and the magnetic field treatment is changed from being repeated for 7 days to being repeated for 2 days. The rest is the same.

[0057] Comparative Example 6 Compared with Example 1, the difference is that in step S2, the magnetic field treatment is performed for the first 7 days of storage at 15°C, and the magnetic field treatment is not performed for the last 7 days, and the rest is the same. Figure 5 shown.

[0058] Comparative Example 7 The epimedium seeds to be sown are obtained by adopting a storage method for epimedium seeds provided in Example 1 of the prior art CN116897776A.

[0059] Comparative Example 8 The dried epimedium seeds are washed, dried, soaked and scrubbed in warm water, and then directly sown as the epimedium seeds to be sown.

[0060] Effect evaluation 1. Sand bed planting experiment.

[0061] Referring to the method provided by the prior art, the epimedium seeds to be sown provided by Examples 1 to 17 and Comparative Examples 1 to 8 were planted in a sand bed. The details are as follows: The number of Epimedium seeds (with sand) to be sown in each group was 100, sown on a sand bed with a humidity of 65% and a daily light exposure of 16 hours. The day when the seeds were sown on the sand bed was counted as day 0, and the number of seeds that germinated on the 6th, 13th, and 20th days was counted to calculate the seed germination rate.

[0062] The formula for calculating the germination rate of Epimedium seeds is as follows: Germination rate = number of germinations ÷ number of seeds sown × 100%.

[0063] The germination rates of Epimedium seeds treated by the treatment methods provided in the embodiments and comparative examples on the 6th, 13th and 20th days of planting in the sand bed are shown in Table 1: Table 1

[0064] It can be seen that, on the one hand, the use of magnetic field to treat epimedium seeds during gradient cooling and sand storage can effectively increase the germination rate of epimedium seeds, and compared with the existing technology, the physiological dormancy time of epimedium seeds is also reduced from 90-95 days to 70-75 days; on the other hand, the best setting method for magnetic field to enhance epimedium seed germination during gradient cooling is to perform magnetic field treatment every day for 5-9 days after being stored at 15°C for two weeks. The magnetic field treatment days are too many, too few, or the magnetic field treatment is not performed for a sufficient time at 15°C. The good technical effect of increasing the seed germination rate cannot be achieved. Epimedium seeds that have not undergone any sand storage and magnetic field treatment are still dormant, and no germination is observed within 20 days after sowing.

[0065] 2. Greenhouse planting experiment.

[0066] Referring to the method of Example 5 in the prior art CN116897776B, a sowing experiment was carried out on the epimedium seeds treated by the epimedium seed treatment method provided in Example 1. After land preparation, 800 seeds to be sown after treatment were evenly sown per square meter of land, covered with 2-3 cm thick earthworm soil, and the seeds and sand were thoroughly watered. The temperature in the shed was maintained at 25°C and the humidity was 65%. The seed germination rate was estimated by the method of counting the number of germinations by random sampling.

[0067] Seed germination rate =

[0068] Wherein, i is an integer and 1≤i≤n, n is the total number of sampling plots; bi is the number of sprouts in the i-th plot, in units; Si is the area of ​​the i-th plot, in square decimeters.

[0069] According to statistics, the epimedium seeds to be sown treated by the epimedium seed processing method provided in Example 1 had a seed germination rate of more than 80% after 15 days of sowing, and more than 90% after 20 days of sowing, and the germination speed was significantly improved.

[0070] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for processing epimedium seeds, characterized in that: The following steps are involved: S1. Mixing epimedium seeds with sand, adding a fungicide solution, and stirring to obtain sand-hidden epimedium seeds; S2, bagging the epimedium seeds obtained in step S1, storing them at 15° C. for 14 days, subjecting the epimedium seeds to magnetic field treatment every day for the last 5-9 days within the 14 days of storage, lowering the storage temperature to 13° C., and gradually cooling the storage temperature by lowering it by 2° C. every two weeks until the storage temperature drops to 5° C., thereby obtaining low-temperature epimedium seeds; S3, storing the low-temperature epimedium seeds obtained in step S2 at a constant temperature and humidity, subjecting the low-temperature epimedium seeds to magnetic field treatment once every 2-4 days of storage, replenishing water, and storing for 70-80 days to obtain epimedium seeds to be sown.

2. The processing method according to claim 1, characterized in that: In step S1, the epimedium seeds are pretreated.

3. The processing method according to claim 1, characterized in that: In step S1, the volume ratio of the epimedium seeds to the sand is 19-21:

1.

4. The processing method according to claim 1, characterized in that: In step S2, the duration of the magnetic field treatment is 10-50 min, and the magnetic field intensity of the magnetic field treatment is 17.6-24.5 mT.

5. The processing method according to claim 4, characterized in that: In step S2, the duration of the magnetic field treatment is 30 minutes, and the magnetic field intensity of the magnetic field treatment is 21.0 mT.

6. The processing method according to claim 1, characterized in that: In step S2, the Herba Epimedii hupensis seeds are subjected to magnetic field treatment every day for the last 7 days within the 14 days of storage.

7. The processing method according to claim 1, characterized in that: In step S3, the duration of the magnetic field treatment is 10-50 min, and the magnetic field intensity of the magnetic field treatment is 5.3-12.5 mT.

8. The processing method according to claim 7, characterized in that: In step S3, the duration of the magnetic field treatment is 30 minutes, and the magnetic field intensity of the magnetic field treatment is 8.9 mT.

9. The processing method according to claim 1, characterized in that: In step S3, the low-temperature epimedium seeds are treated with a magnetic field once every 3 days of storage.

10. Use of the treatment method according to any one of claims 1 to 9 in epimedium cultivation.

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