One-step preparation of dopamine polymer with near-infrared Ⅱ region light absorption ability and application thereof

Abstract

The application belongs to the technical field of biological medicine, and particularly relates to a one-step preparation method of a dopamine polymer with near-infrared II region light absorption capacity and application thereof. The preparation method comprises the following steps: dissolving cobalt nitrate in an ethanol aqueous solution containing 2-methyl imidazole, adding dopamine hydrochloride into the solution, stirring at room temperature, carrying out the reaction through a water heating or ultrasonic method for a period of time, and obtaining the dopamine polymer after the reaction is completed. The material has strong light absorption characteristics in the near-infrared II region through a simple synthesis method, has adjustable photothermal and photodynamic dual functions, and has excellent sterilization capacity.

Description

Technical Field

[0001] This invention belongs to the field of biomedical technology, specifically relating to a one-step preparation of a dopamine polymer with near-infrared II region light absorption capability and its photothermal / photodynamic antibacterial application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Bacterial infections pose a serious threat to human health, and antibiotic resistance is gradually becoming a global public health crisis. Bacterial resistance severely weakens the therapeutic effects of traditional antibiotics, making the development of novel antibacterial strategies a major challenge urgently needing to be addressed in the global biomedical field. Optical therapy, as an emerging non-invasive treatment method, especially photothermal and photodynamic therapy, has attracted much attention due to its unique treatment mechanism, which can effectively avoid the problem of bacterial resistance.

[0004] Near-infrared light (NIR, 780-1700nm) induces photothermal conversion and photochemical reactions that can effectively kill pathogens without damaging normal tissues. It also penetrates the skin and other tissues well to reach lesions, thus holding great potential for in vivo disease treatment. NIR II region light (1000-1700nm) exhibits greater advantages due to its higher biocompatibility and deeper tissue penetration. However, existing NIR light-absorbing materials still face several challenges in realizing phototherapy applications. First, there are relatively few materials capable of efficiently absorbing NIR light. Second, the preparation process of these materials typically involves complex chemical synthesis steps, increasing production costs and limiting their large-scale production and application. Furthermore, the effectiveness of single therapies in phototherapy is often limited; materials with both photothermal and photodynamic functions can better exert therapeutic effects, but the regulation of these dual functions is currently difficult, and single materials possessing both photothermal and photodynamic functions are rare, often requiring the combined use of different materials.

[0005] The current market lacks multifunctional materials that can simultaneously meet the above requirements, which not only limits the further development of optical therapy technology but also affects its widespread clinical application. Summary of the Invention

[0006] To address the aforementioned problems, this invention provides a one-step preparation method for a dopamine polymer with near-infrared II light absorption capability and its photothermal / photodynamic antibacterial application. This material, through a simple synthesis method, exhibits strong light absorption characteristics in both the near-infrared I and II bands, possessing both photothermal and photodynamic functions, and can effectively kill bacteria.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a one-step method for preparing a dopamine polymer with near-infrared II region light absorption capability, comprising:

[0009] Cobalt salt and 2-methylimidazole were dissolved in an organic solvent to obtain a mixture containing cobalt salt and 2-methylimidazole.

[0010] Dopamine hydrochloride is added to the mixture containing cobalt salt and 2-methylimidazole, and the reaction is carried out under preset conditions to obtain a dopamine polymer.

[0011] In some embodiments, the cobalt salt is cobalt nitrate.

[0012] In some embodiments, the molar ratio of the cobalt salt to 2-methylimidazole is 1:50 to 60, or 1:55.

[0013] In some embodiments, the molar ratio of the cobalt salt to dopamine hydrochloride is 1:0.15 to 1:150, or 1:1.5.

[0014] In some embodiments, the organic solvent is an aqueous solution of ethanol.

[0015] In some embodiments, the volume ratio of ethanol to water is 2:1 to 2:5, or 2:3.

[0016] In some embodiments, the preset conditions are stirring at room temperature, hydrothermal reaction, or ultrasonic reaction.

[0017] In some embodiments, the stirring time at room temperature is 3 to 5 hours, or 4 hours.

[0018] In some embodiments, the hydrothermal reaction is carried out at 110–130°C for 2–6 hours, or at 120°C for 4 hours.

[0019] In some implementations, the ultrasonic treatment lasts for 20-60 minutes, or 40 minutes.

[0020] More specifically, including:

[0021] Cobalt nitrate was dissolved in an aqueous ethanol solution containing 2-methylimidazole, and dopamine hydrochloride was added to it. The reaction was carried out for a period of time by stirring, hydrothermal treatment or ultrasound at room temperature. After the reaction was completed, a dopamine polymer was obtained.

[0022] In a second aspect, the present invention provides a dopamine polymer with near-infrared II region light absorption capability prepared by the above method.

[0023] A third aspect of the present invention provides the use of the above-described dopamine polymer in the preparation of photothermal / photodynamic antibacterial materials or devices.

[0024] Beneficial effects of the present invention

[0025] (1) This invention prepares dopamine polymer materials in one step. The preparation method is simple, the process is simplified, and the experimental steps are reduced. At the same time, the preparation methods are diversified and the operation is easier to implement.

[0026] (2) The dopamine polymer material prepared by this invention covers the ultraviolet-visible-near-infrared light absorption range and can effectively absorb light with wavelengths above 1500 nm. This improvement enhances the material's ability to effectively utilize light, especially giving it excellent performance in the near-infrared II region, effectively broadening the material's application range.

[0027] (3) The dopamine polymer material prepared by this invention has dual photothermal and photodynamic functions under laser irradiation, enabling it to generate local high temperatures through photothermal effects and to generate reactive oxygen species (ROS) through photodynamic effects for antibacterial purposes during the photoresponse process. This dual-functionality can significantly enhance the material's application effect in sterilization and greatly improve sterilization efficiency. Attached Figure Description

[0028] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. Exemplary embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0029] Figure 1 The images show the ultraviolet-visible-near-infrared absorption spectra of the nanomaterials prepared in Examples 1-12 of this invention; wherein, a is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 1-4, b is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 5-8, c is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 9-12, and d is the ultraviolet-visible-near-infrared absorption spectrum of the samples dispersed in water by ultrasonication in Examples 1-4.

[0030] Figure 2 The X-ray diffraction (XRD) patterns of the nanomaterials prepared in Examples 1 to 4 of this invention are shown below.

[0031] Figure 3 The Fourier transform infrared (FTIR) spectra of the nanomaterials prepared in Examples 1-4 of this invention are shown below.

[0032] Figure 4 This is the full X-ray photoelectron spectroscopy (XPS) spectrum of Example 1 of the present invention;

[0033] Figure 5 These are scanning electron microscope (SEM) images of the nanomaterials prepared in Examples 1 to 4 of the present invention; wherein, a is the SEM image of Example 1, b is the SEM image of Example 2, c is the SEM image of Example 3, and d is the SEM image of Example 4.

[0034] Figure 6 These are transmission electron microscope (TEM) images of the nanomaterials prepared in Examples 1 to 4 of the present invention; wherein, a is the TEM image of Example 1, b is the TEM image of Example 2, c is the TEM image of Example 3, and d is the TEM image of Example 4.

[0035] Figure 7 The nanomaterials prepared in Examples 1-4 of this invention are subjected to NIR light at different wavelengths (1.0 W / cm²). 2 Temperature changes after 10 minutes of irradiation; where a is the temperature change under 808nm NIR I region irradiation and b is the temperature change under 1064nm NIR II region irradiation.

[0036] Figure 8 The nanomaterials prepared in Examples 1-4 of this invention were dispersed in water (concentration of 1 mg / mL) after ultrasonic treatment and then subjected to NIR I light (808 nm, 1.0 W / cm²). 2 Infrared thermal images of the material heated to its maximum temperature after 10 minutes of irradiation; wherein, a is the infrared thermal image of the material obtained in Example 1, b is the infrared thermal image of the material obtained in Example 2, c is the infrared thermal image of the material obtained in Example 3, and d is the infrared thermal image of the material obtained in Example 4.

[0037] Figure 9 The images show the fluorescence emission spectra of the nanomaterials prepared in Examples 1-4 of this invention dispersed in an aqueous solution and after the addition of the reactive oxygen species probe DCFH-DA; where a represents the fluorescence emission spectrum of Example 1 in the NIR I region (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes are shown in Figure b, which is the fluorescence emission spectrum of Example 2 under NIR I region light (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes are shown in Figure c, where c represents the fluorescence emission spectrum of Example 3 under NIR I region light (808 nm, 1.0 W / cm²). 2Fluorescence emission spectra after irradiation for 0 and 5 minutes, d is the fluorescence emission spectrum of Example 4 under NIR I region light (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes;

[0038] Figure 10 This is a diagram showing the antibacterial effect of the nanomaterials prepared in Example 1 of this invention after being irradiated with an 808nm laser in the NIR I region;

[0039] Figure 11 This image shows the effect of the nanomaterials prepared in Example 1 of the present invention on Staphylococcus aureus after being irradiated by a 1064nm NIR II region laser.

[0040] Figure 12 This is a schematic diagram illustrating the preparation of the material described in this invention and its photothermal / photodynamic antibacterial properties. Detailed Implementation

[0041] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0042] The present invention will be further described in detail below with reference to specific embodiments. It should be noted that the specific embodiments are explanations of the present invention and not limitations thereof.

[0043] Example 1

[0044] A one-step method for preparing dopamine polymers with near-infrared II light absorption capability includes the following steps:

[0045] (1) 29.2 mg of cobalt nitrate hexahydrate, 454.0 mg of 2-methylimidazole, and 292.0 mg of dopamine hydrochloride were dissolved in an ethanol-water solution containing 4 mL of ethanol and 6 mL of deionized water. The solution was stirred at room temperature for 4 h, centrifuged to collect the product, washed three times with ethanol, and dried in a drying oven at 60 °C for 12 h to obtain the dopamine polymer material. The molar ratio of cobalt nitrate hexahydrate to dopamine hydrochloride was 1:1.5.

[0046] Example 2

[0047] The difference from Example 1 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:0.15, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 2.9 mg of dopamine hydrochloride are added.

[0048] Example 3

[0049] The difference from Example 1 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:15, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 29.2 mg of dopamine hydrochloride are added.

[0050] Example 4

[0051] The difference from Example 1 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:150, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 584.0 mg of dopamine hydrochloride are added.

[0052] Example 5

[0053] (1) 29.2 mg of cobalt nitrate hexahydrate, 454.0 mg of 2-methylimidazole, and 2.9 mg of dopamine hydrochloride were dissolved in an ethanol-water solution containing 4 mL of ethanol and 6 mL of deionized water to obtain a mixture. The mixture was placed in a 25 mL high-pressure sealed reactor and subjected to a hydrothermal reaction at 120 °C for 4 h. After the reaction was completed, the mixture was allowed to cool naturally, and the resulting sample was collected by centrifugation. The product was then washed three times with ethanol and dried in a 60 °C drying oven for 12 h to obtain the dopamine polymer material. The molar ratio of cobalt nitrate hexahydrate to dopamine hydrochloride was 1:0.15.

[0054] Example 6

[0055] The difference from Example 5 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:1.5, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 29.2 mg of dopamine hydrochloride are added.

[0056] Example 7

[0057] The difference from Example 5 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:15, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 292.0 mg of dopamine hydrochloride are added.

[0058] Example 8

[0059] The difference from Example 5 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:150, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 584.0 mg of dopamine hydrochloride are added.

[0060] Example 9

[0061] (1) 29.2 mg of cobalt nitrate hexahydrate, 454.0 mg of 2-methylimidazole, and 2.9 mg of dopamine hydrochloride were dissolved in an ethanol-water solution containing 4 mL of ethanol and 6 mL of deionized water to obtain a mixture. The mixture was sonicated for 40 min. After the reaction was completed, the resulting sample was centrifuged to collect the product, washed three times with ethanol, and dried in a 60 °C drying oven for 12 h to obtain the dopamine polymer material. The molar ratio of cobalt nitrate hexahydrate to dopamine hydrochloride was 1:0.15.

[0062] Example 10

[0063] The difference from Example 9 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:1.5, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 29.2 mg of dopamine hydrochloride are added.

[0064] Example 11

[0065] The difference from Example 9 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:15, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 292.0 mg of dopamine hydrochloride are added.

[0066] Example 12

[0067] The difference from Example 9 is that the molar ratio of cobalt nitrate hexahydrate and dopamine hydrochloride in step (1) is adjusted to 1:150, that is, in step (1), 29.2 mg of cobalt nitrate hexahydrate and 584.0 mg of dopamine hydrochloride are added.

[0068] The light absorption properties of the nanomaterials prepared in Examples 1-12 of this invention were tested:

[0069] Figure 1 The images show the ultraviolet-visible-near-infrared absorption spectra of the nanomaterials prepared in Examples 1-12 of this invention; where a is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 1-4, b is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 5-8, c is the ultraviolet-visible-near-infrared absorption spectrum of the solid samples obtained in Examples 9-12, and d is the ultraviolet-visible-near-infrared absorption spectrum of the samples dispersed in water by ultrasound in Examples 1-4. All 12 samples have strong light absorption capabilities in the ultraviolet-visible-near-infrared region, and the light absorption region of the samples can reach above 1500 nm, indicating that they have the basis for optical applications in the near-infrared II region.

[0070] The nanomaterials prepared in Examples 1-4 of this invention were characterized in terms of material structure and morphology:

[0071] in, Figure 2The images shown are XRD patterns of the nanomaterials prepared in Examples 1-4 of this invention. Figure 2 As can be seen, when the dopamine hydrochloride content is the lowest, Example 2 shows obvious characteristic peaks of the metal-organic framework material ZIF-67. However, as the dopamine hydrochloride content further increases, the intensity of the diffraction peaks in Example 1 is weakened compared to Example 2, and the crystallinity decreases. When the dopamine hydrochloride content continues to increase, Example 4 eventually transforms into an amorphous structure.

[0072] Figure 3 These are the FTIR spectra of the nanomaterials prepared in Examples 1-4 of this invention. Figure 3 As can be seen from the four examples, the materials prepared at 1299 cm⁻¹ -1 1510cm -1 and 1600cm -1 Distinct characteristic absorption peaks appear at 3100–3687 cm⁻¹, corresponding to the phenol CO group, amino group, and benzene ring group, respectively. -1 The broad absorption bands within the range are attributed to the stretching vibrations of the NH and OH bonds, and these characteristic peaks indicate the presence of polydopamine; the material prepared in Example 2 showed a peak at 756 cm⁻¹. -1 The peak value is the absorption peak of the Co-N bond stretching vibration, 995–1301 cm⁻¹. -1 The absorption peaks are for the bending vibrations of the imidazole ring and the out-of-plane bending vibrations, ranging from 1301 to 1508 cm⁻¹. -1 The absorption peak within the range corresponds to the stretching vibration of the imidazole ring, at 1574 cm⁻¹. -1 The peak value is the absorption peak of the C=N bond in the imidazole ring, which belongs to the characteristic peak of ZIF-67. At this time, the dopamine hydrochloride content is the lowest. As the dopamine hydrochloride content increases, the characteristic peak of ZIF-67 gradually disappears while the characteristic peak of the dopamine polymer becomes more obvious. This result shows that the microstructure of the material can be effectively controlled by controlling the ratio of reactants.

[0073] Figure 4 This is the XPS full spectrum of Embodiment 1 of the present invention; from Figure 4 As can be seen from the full spectrum, four characteristic peaks of Co 2p, C 1s, N 1s and O 1s are displayed, which also proves the successful preparation of dopamine polymer;

[0074] Figure 5 These are SEM images of the nanomaterials prepared in Examples 1-4 of this invention; wherein, a is the SEM image of Example 1, b is the SEM image of Example 2, c is the SEM image of Example 3, and d is the SEM image of Example 4; from Figure 5 As can be seen from Figure a, the material in Example 1 is an amorphous cluster structure formed by the aggregation of nanoparticles, with a clear structure; from Figure 5As can be seen in Example 2 (b), the surface is relatively rough, partially exhibiting the structural characteristics of ZIF-67, and the particle size is relatively uniform. With increasing dopamine hydrochloride content, the surface of the nanoparticles becomes increasingly smooth, showing obvious morphological changes, such as... Figure 5 As shown in c and d, the material surface becomes smooth and rounded, the coating layer thickens significantly, resulting in increased particle size and more regular particle shape;

[0075] Figure 6 These are TEM images of the nanomaterials prepared in Examples 1-4 of this invention, where a is a TEM image of Example 1, b is a TEM image of Example 2, c is a TEM image of Example 3, and d is a TEM image of Example 4; from Figure 6 As can be seen in Example 1, the nanoparticles aggregate into clusters. With increasing dopamine hydrochloride content, the particles gradually increase in size, become more regular in shape, and have a smoother surface. In Example 2, with the lowest dopamine hydrochloride content, some particles exhibit a hexagonal structure, indicating the presence of ZIF-67. As the dopamine hydrochloride content increases, the morphology gradually evolves into more regular spherical or elliptical shapes, indicating more pronounced polymer characteristics. Figure 6 As shown in Example 4 of the middle d;

[0076] The photothermal and photodynamic properties of the nanomaterials prepared in Examples 1-4 of this invention were tested:

[0077] Photothermal property testing

[0078] Figure 7 The nanomaterials prepared in Examples 1-4 of this invention are subjected to NIR light at different wavelengths (1.0 W / cm²). 2 Temperature changes after 10 minutes of irradiation; where a represents the temperature change under 808nm NIR I region irradiation, and b represents the temperature change under 1064nm NIR II region irradiation; Figure 7 It can be seen that the temperature increases significantly with the increase of irradiation time;

[0079] Figure 8 The nanomaterials prepared in Examples 1-4 of this invention were dispersed in water (concentration 1 mg / mL) after ultrasonic treatment, and then subjected to NIR I light (808 nm, 1.0 W / cm²). 2 Infrared thermal images of the material heated to its maximum temperature after 10 minutes of irradiation; where a is the infrared thermal image of the material obtained in Example 1, b is the infrared thermal image of the material obtained in Example 2, c is the infrared thermal image of the material obtained in Example 3, and d is the infrared thermal image of the material obtained in Example 4; the excellent photothermal conversion performance of the material can also be observed more intuitively from the infrared thermal images.

[0080] Photodynamic properties (ROS generation capability) test

[0081] Figure 9 The images show the fluorescence emission spectra of the nanomaterials prepared in Examples 1-4 of this invention dispersed in an aqueous solution and after the addition of the reactive oxygen species probe DCFH-DA; where a represents the fluorescence emission spectrum of Example 1 in the NIR I region (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes are shown in Figure b, which is the fluorescence emission spectrum of Example 2 under NIR I region light (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes are shown in Figure c, where c represents the fluorescence emission spectrum of Example 3 under NIR I region light (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes, d is the fluorescence emission spectrum of Example 4 under NIR I region light (808 nm, 1.0 W / cm²). 2 Fluorescence emission spectra after irradiation for 0 and 5 minutes; as shown Figure 9 As shown, with the extension of irradiation time, the fluorescence intensity of all examples gradually increased but the increase was different. This indicates that the materials obtained in Examples 1 to 4 have the ability to generate ROS, especially Example 1, which has the most outstanding ability to generate ROS. The peak fluorescence intensity of its solution increased from 1251 to 70131 (56 times).

[0082] The antibacterial properties of the nanomaterials prepared in Example 1 of this invention were tested:

[0083] The photothermal and photodynamic property test results of the material prepared in Example 1 show that it has a synergistic antibacterial effect of photothermal and photodynamic. Escherichia coli and Staphylococcus aureus were selected to verify its antibacterial effect.

[0084] Figure 10 The nanomaterials prepared in Example 1 of this invention are used in the NIR I region (808 nm, 1.0 W / cm²) light. 2 The following is a comparison chart of antibacterial activity, using E. coli (2×10⁻⁶). 9 The solution (CFU / mL) was mixed with aqueous solutions of the material obtained in Example 1 at concentrations of 0 μg / mL, 100 μg / mL, and 200 μg / mL, respectively. Bacterial cultures were then performed after irradiation with NIR I zone light for 0, 10, and 20 min, and bacterial growth was observed visually after 24 h. The results showed that when the bacterial solution without the obtained material was irradiated with a laser, no significant bactericidal effect was observed within 20 min. However, when the solution contained the prepared material, a significant antibacterial effect was observed against Escherichia coli, and the bactericidal effect was concentration-dependent. At a material concentration of 200 μg / mL, the bacteria were completely killed within 20 min.

[0085] Figure 11The nanomaterials prepared in Example 1 of this invention exhibited performance in the NIR II region (1064 nm, 1.0 W / cm²). 2 The image shows the effect of irradiation on Staphylococcus aureus. Staphylococcus aureus (2×10⁻⁶) was collected. 9 The CFU / mL solution was mixed with aqueous solutions of the material obtained in Example 1 at concentrations of 0 μg / mL, 100 μg / mL, and 200 μg / mL, respectively. Bacterial culture was performed after irradiation with NIR II zone light for 0, 10, and 20 min, and bacterial growth was observed after 24 h. This yielded results comparable to... Figure 10 Similar experimental results indicate that the obtained material can effectively kill bacteria within a short time under laser irradiation. This demonstrates that the material prepared in this invention is a potentially high-performance novel antibacterial material.

[0086] Figure 12 This is a schematic diagram illustrating the preparation of the material described in this invention and its photothermal / photodynamic antibacterial properties, as shown below. Figure 12 As shown, the material was prepared by mixing cobalt nitrate, 2-methylimidazole and dopamine hydrochloride. The prepared sample exhibited excellent photothermal conversion performance and could generate ROS under irradiation with 808nm and 1064nm lasers, thereby achieving the purpose of antibacterial activity.

[0087] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A one-step method for preparing a dopamine polymer with near-infrared II region light absorption capability, characterized in that, include: Cobalt salt and 2-methylimidazole were dissolved in an organic solvent to obtain a mixture containing cobalt salt and 2-methylimidazole. Dopamine hydrochloride is added to the mixture containing cobalt salt and 2-methylimidazole, and the reaction is carried out under preset conditions to obtain a dopamine polymer, which is the final product. The molar ratio of the cobalt salt to dopamine hydrochloride is 1:0.15 to 1:150; The dopamine polymer has both photothermal and photodynamic functions under laser irradiation; The preset conditions are stirring at room temperature, hydrothermal reaction, or ultrasonic reaction; The stirring time at room temperature is 3-5 hours; The hydrothermal reaction is carried out at 110-130 °C for 2-6 h. Ultrasonic treatment for 20-60 minutes; The cobalt salt is cobalt nitrate; The molar ratio of the cobalt salt to 2-methylimidazole is 1:50~60.

2. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, The molar ratio of the cobalt salt to 2-methylimidazole is 1:

55.

3. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, The molar ratio of the cobalt salt to dopamine hydrochloride is 1:1.

5.

4. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, The organic solvent is an aqueous solution of ethanol.

5. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 4, characterized in that, The volume ratio of ethanol to water is 2:1 to 2:

5.

6. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 4, characterized in that, The volume ratio of ethanol to water is 2:

3.

7. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, The stirring time at room temperature is 4 hours.

8. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, The hydrothermal reaction was carried out at 120 °C for 4 h.

9. The one-step preparation method of the dopamine polymer with near-infrared II region light absorption capability as described in claim 1, characterized in that, Ultrasonic treatment for 40 min.

10. The dopamine polymer with near-infrared II region light absorption capability prepared by the method according to any one of claims 1-9.

11. The use of the dopamine polymer of claim 10 in the preparation of photothermal / photodynamic antibacterial materials or devices.

Images