Multi-channel field stimulation electrode array system

Abstract

The invention relates to the technical field of medical instruments and biomedical engineering, and discloses a multichannel field stimulation electrode array system, which is characterized in that culture test tubes are uniformly arranged on a culture plate, and a stimulation electrode array unit is assembled in a cover plate; the bottom of the cover plate is fixedly provided with a positive electrode unit and a negative electrode unit which correspond to the culture test tubes, the positive electrode unit and the negative electrode unit are connected with the stimulation electrode array unit, different stimulation effects are achieved by controlling different channel fields, and the culture plate unit is provided with an electrode array with a plurality of independent channels. Each channel can be independently controlled, multi-channel point stimulation is achieved, flux is improved, an electrical stimulation field is generated in the culture test tube, mutual interference is avoided, independence is high, in addition, the intensity of the electrical stimulation field in the group of culture test tubes can be changed, and therefore the regulation and control requirements for giving different electrical stimulation when the device is used for culturing different cells or tissues can be met.

Description

Technical Field

[0001] The present invention relates to the field of medical equipment technology and biomedical engineering technology, and in particular to a multi-channel field stimulation electrode array system. Background Art

[0002] At present, electrical stimulation technology has been widely used in preclinical research for biological mechanism exploration, medicinal mechanism research, drug screening, drug efficacy evaluation, etc., especially for cardiomyocytes and nerve cells.

[0003] In an extraocular electrode array device for local electrical stimulation of the retina with application number CN201910648942.3, electrical stimulation signals of different parameters are transmitted according to actual stimulation needs, and a direct stimulation mode is used for large-area electrical stimulation or an interference stimulation mode is used for local electrical stimulation. It can be used for electrical stimulation treatment of local areas of the retina, and can also be used as a stimulation method for minimally invasive retinal prostheses to induce artificial visual perception of a larger field of view.

[0004] However, traditional electrical stimulation technology often uses a single channel or a few channels, and the stimulation effect is limited. If high-intensity regional stress stimulation is used, it will easily cause irreversible damage. At the same time, it is easy to have problems such as difficulty in accurately controlling the stimulation area and inflexible adjustment of stimulation parameters, which limits its application effect in complex scenarios. With the continuous development of medical technology, the requirements for the accuracy and controllability of electrical stimulation technology are becoming higher and higher.

[0005] Therefore, the present invention solves the above-mentioned problems through a multi-channel field stimulation electrode array system. Summary of the invention

[0006] The purpose of the present invention is to provide a multi-channel field stimulation electrode array system to solve the problems raised in the above-mentioned background technology. The multi-channel electrical stimulation technology can provide more accurate and flexible stimulation schemes, improve the accuracy, controllability, diversity, and high throughput of the electrical stimulation technology, and meet the needs of different experimental conditions. As an innovative electrical stimulation electrode, the array electrode has the advantages of multi-channel, high density, and programmability, and can achieve more precise electrical stimulation control.

[0007] To achieve the above-mentioned object, the present invention provides the following technical solutions: a multi-channel field stimulation electrode array system, comprising a culture plate unit, the culture plate unit comprising a culture plate and a cover plate, the culture plate is evenly provided with culture tubes, the cover plate is equipped with a stimulation electrode array unit, the bottom of the cover plate is fixed with a positive electrode unit and a negative electrode unit corresponding to the culture tubes, and the positive electrode unit, the negative electrode unit and the stimulation electrode array unit are connected; The stimulation electrode array unit includes a positive electrode and a negative electrode fixed on the left side wall of the cover plate, the positive electrode is connected to a main positive electrode line laid inside the cover plate, the negative electrode is connected to a main negative electrode line laid inside the cover plate, the main positive electrode line is connected to positive electrode branches with the same number of culture test tube columns, the main negative electrode line is connected to negative electrode branches with the same number of culture test tube columns, and each group of positive electrode units is connected to the positive electrode branches through a conductive wire, and each group of negative electrode units is connected to the negative electrode branches through a conductive wire.

[0008] Preferably, the culture plate unit also includes a liquid filling port opened on the surface of the culture plate and corresponding one-to-one to the culture test tube, a power connector is fixed in the middle of the left side of the cover plate, the power connector connects the positive pole and the negative pole, and a receiving groove corresponding to the positive electrode unit and the negative electrode unit is opened at the bottom of the cover plate.

[0009] Preferably, a packaging ring corresponding to the culture tube is fixed at the bottom of the cover plate, the positive electrode unit and the negative electrode unit are assembled inside the packaging ring, the positive electrode unit and the negative electrode unit have the same composition structure and are relatively arranged, and the packaging ring matches the liquid filling port.

[0010] Preferably, the positive electrode unit comprises a first bracket and a first mounting frame fixed on the inner left wall of the packaging ring, and a group of insulating cylinders are hingedly assembled on the front and rear sides of the inner wall of the first bracket through a movable axis, and a positive platinum electrode rod is fixedly assembled inside the group of insulating cylinders.

[0011] Further preferably, a first pushing member is fixed on the left inner wall of the first mounting frame, a first limiting member is fixed on the right inner wall of the first mounting frame, and the other sides of the first pushing member and the first limiting member are fixedly mounted on the outer wall of the positive platinum electrode rod.

[0012] Preferably, the negative electrode unit comprises a second bracket and a second mounting frame fixed on the right inner wall of the packaging ring, and another group of insulating cylinders are hingedly assembled on the front and rear sides of the inner wall of the second bracket through a movable axis, and a negative platinum electrode rod is fixedly assembled inside the group of insulating cylinders.

[0013] Further preferably, a second pushing member is fixed on the right side wall inside the second mounting frame, a second limiting member is fixed on the left side wall inside the second mounting frame, and the other side of the second pushing member and the second limiting member are fixedly mounted on the outer side wall of the negative platinum electrode rod.

[0014] Preferably, the first pushing member, the first limiting member, the second pushing member, and the second limiting member are all pressure-bearing air bags, and the first pushing member, the first limiting member, the second pushing member, and the second limiting member are respectively connected to the air pump through the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe, and the first solenoid valve, the second solenoid valve, the third solenoid valve, and the fourth solenoid valve are respectively fixedly assembled on the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe.

[0015] Preferably, the positive platinum electrode rod is connected to the conductive wire on the positive branch line, the negative platinum electrode rod is connected to the conductive wire on the negative branch line, and the positive platinum electrode rod and the negative platinum electrode rod have the same length and extend into the culture tube together.

[0016] Preferably, a multi-channel field stimulation electrode array system also includes an incubator, wherein the inner wall of the incubator is provided with a rack for supporting and limiting the culture plate units, and the number of the racks is the same as the number of culture plate units, and a power plug matching the power connector is fixed on the inner wall of the incubator.

[0017] Preferably, a door is hingedly mounted on the front side wall of the incubator, an electric control box is fixed on the top of the incubator, and a display screen matching the culture plate unit is installed on the front side wall of the electric control box.

[0018] Technical effects and advantages of the present invention: 1. In the present invention, when cell fluid is introduced into the culture tube, the positive electrode unit and the negative electrode unit are energized through the stimulation electrode array unit. A group of culture tubes is an independent electrode channel. In this way, under the driving action of the stimulation electrode array unit, each electrode channel on the culture plate is independently controlled to prevent the culture tubes from interfering during culture and affecting the accuracy of cell status detection. During culture, the stimulation electrode array unit is controlled to stimulate cells in different areas to different degrees, and the results of the cell or tissue response to the stimulation are recorded, thereby improving the accuracy and controllability of the electrical stimulation technology.

[0019] 2. The culture plate in the present invention can cooperate with the stimulation electrode array unit on the cover plate to form a stimulation electrode array with a multi-channel field, and different stimulation effects can be achieved by controlling different channel fields. The number of culture tubes in the culture plate unit can be customized according to the culture needs, which is convenient for horizontal comparison of different cells during culture and improves the detection accuracy of cells under stress stimulation. During culture, the culture plate unit has an electrode array with multiple independent channels, each channel can be independently controlled to achieve multi-channel point stimulation and improve flux. An electrical stimulation field is generated inside the culture tube without interfering with each other and with strong independence. At the same time, the current value generated in the corresponding culture tube can be changed, and then the strength of the electrical stimulation field inside the group of culture tubes can be changed, thereby meeting the regulation needs of the device for giving different electrical stimulations when culturing different cells or tissues.

[0020] 3. In order to detect the stimulating effect of platinum electrodes on cells when generating different electrical stimulation fields, the present invention can send a control signal to the air pump through the stimulation chip in the electrical control box during actual use, and use the cooperation of the air pump and the solenoid valve to control the positive electrode unit and the negative electrode unit, so as to facilitate the adjustment of the relative position between the positive platinum electrode rod and the negative platinum electrode rod, thereby changing the distribution pattern of the electrical stimulation field in the culture tube and giving cells or tissues more electrical stimulation.

[0021] 4. The present invention detects the use status of the platinum electrode based on the change of the current value in a single circuit of the platinum electrode, so as to reflect the passivation degree of the positive platinum electrode rod and the negative platinum electrode rod, facilitate real-time adjustment measures, improve the protection of the platinum electrode, and improve the detection accuracy during cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the internal structure of the incubator of the present invention; Figure 3 This is a schematic diagram of the structure of the culture plate unit of the present invention; Figure 4 This is a schematic diagram of the split structure of the culture plate unit of the present invention; Figure 5 This is a schematic diagram of the overall structure of the cover plate of the present invention; Figure 6 It is a schematic diagram of the local structure of the cover plate of the present invention; Figure 7 A bottom view of the assembly structure of the positive electrode unit and the negative electrode unit of the present invention; Figure 8 A top view of the assembly structure of the positive electrode unit and the negative electrode unit of the present invention; Fig. 9This is a schematic diagram of the structural layout of the stimulation electrode array unit of the present invention.

[0023] In the figure: 10, incubator; 11, door; 12, electric control box; 13, display screen; 14, shelf; 15, power plug; 20, culture plate unit; 21, culture plate; 22, culture tube; 23, liquid filling port; 24, cover plate; 25, power connector; 26, packaging ring; 27, receiving slot; 30, stimulation electrode array unit; 31, positive electrode; 32, negative electrode; 33, main positive electrode line; 34, main negative electrode line; 35 , positive branch line; 36, negative branch line; 37, conductive line; 40, positive electrode unit; 41, first bracket; 42, first mounting frame; 43, movable shaft; 44, insulating cylinder; 45, positive platinum electrode rod; 46, first pushing member; 47, first limiting member; 50, negative electrode unit; 51, second bracket; 52, second mounting frame; 53, second pushing member; 54, second limiting member; 55, negative platinum electrode rod. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0025] First embodiment: This embodiment provides Figures 1 to 9 A multi-channel field stimulation electrode array system is shown, including a culture plate unit 20, which makes it easy to arrange the number of culture plate units 20 according to actual needs. At the same time, the culture plate unit 20 can be disassembled and replaced, and can be recycled. Setting up multiple culture plate units 20 can realize the culture of multiple groups of cells and the culture of multiple types of cells, which is more operable.

[0026] See also Figure 2-Figure 6 The culture plate unit 20 includes a culture plate 21 and a cover plate 24, culture tubes 22 are evenly arranged on the culture plate 21, a stimulation electrode array unit 30 is assembled in the cover plate 24, a positive electrode unit 40 and a negative electrode unit 50 corresponding to the culture tubes 22 are fixed to the bottom of the cover plate 24, and the positive electrode unit 40, the negative electrode unit 50 and the stimulation electrode array unit 30 are connected.

[0027] The number of culture tubes 22 designed in the culture plate 21 disclosed in this embodiment is 96, which can be designed as 6, 12, 24, 36, 48, 96, 384 groups according to actual needs. The stimulation electrode array can cooperate with the stimulation electrode array unit 30 on the cover plate 24 to form a stimulation electrode array with a multi-channel field. Different stimulation effects can be achieved by controlling different channel fields. Then, when the cell fluid is introduced into the culture tube 22, the positive electrode unit 40 and the negative electrode unit 50 are energized through the stimulation electrode array unit 30. A group of culture tubes 22 is an independent electrode channel. In this way, under the driving action of the stimulation electrode array unit 30, each electrode channel on the culture plate 21 is independently controlled to prevent the culture tubes 22 from interfering during culture and affecting the accuracy of cell status detection. During culture, the stimulation electrode array unit 30 is controlled to stimulate cells in different areas to different degrees, and the results of the cell or tissue response to the stimulation are recorded, thereby improving the accuracy and controllability of the electrical stimulation technology.

[0028] See also Figure 4 and Fig. 9 The stimulation electrode array unit 30 includes a positive electrode 31 and a negative electrode 32 fixed on the left side wall of the cover plate 24. The positive electrode 31 and the negative electrode 32 can ensure the effective operation of the stimulation electrode array unit 30. The positive electrode 31 is connected to a main positive electrode line 33 laid inside the cover plate 24, and the negative electrode 32 is connected to a main negative electrode line 34 laid inside the cover plate 24. The main positive electrode line 33 and the main negative electrode line 34 are laid according to the length of the culture plate 21, which can ensure that each row of culture tubes 22 can be connected to electricity. The main positive electrode line 33 is connected to a positive electrode branch line 35 with the same number of culture tubes 22, and the main negative electrode line 34 is connected to a positive electrode branch line 35 with the same number of culture tubes 22. It is connected with negative branch lines 36 with the same number of culture tubes 22 columns, and the positive branch lines 35 and the negative branch lines 36 match the width of the culture plate 21, which can ensure that each column of culture tubes 22 can be connected to electricity, and each group of positive electrode units 40 is connected to the positive branch line 35 through a conductive line 37, and each group of negative electrode units 50 is connected to the negative branch line 36 through a conductive line 37. When the positive electrode unit 40 inside the culture tube 22 is energized, the positive electrode unit 40 and the negative electrode unit 50 will generate an electric field inside the culture tube 22, thereby achieving the effect of stimulating the cultured cells or tissues.

[0029] See also Figure 3The culture plate unit 20 also includes a liquid filling port 23 opened on the surface of the culture plate 21 and corresponding to the culture test tube 22. A power connector 25 is fixed in the middle of the left side of the cover 24. The power connector 25 connects the positive electrode 31 and the negative electrode 32. A receiving groove 27 corresponding to the positive electrode unit 40 and the negative electrode unit 50 is opened at the bottom of the cover 24. The culture plate unit 20 adopts a detachable design, and the power connector 25 adopts an active plug-in type, which is convenient for operation when replacing the culture plate unit 20. At the same time, the culture plate unit 20 can be quickly powered off without affecting the cell culture of other culture plate units 20.

[0030] See also Figure 4-Figure 7 A packaging ring 26 corresponding to the culture tube 22 is fixed at the bottom of the cover 24, and the packaging ring 26 matches the liquid adding port 23. In this way, when the cover 24 is installed on the top of the culture plate 21, the packaging ring 26 can seal the liquid adding port 23. The positive electrode unit 40 and the negative electrode unit 50 are assembled inside the packaging ring 26. The positive electrode unit 40 and the negative electrode unit 50 have the same structure and are arranged relatively to each other, so that after the positive electrode unit 40 is energized, the electric field generated by the positive electrode unit 40 and the negative electrode unit 50 inside the culture tube 22 can be used to generate electrical stimulation to the cultured cells or tissues.

[0031] See also Figure 7 and Figure 8 The positive electrode unit 40 includes a first bracket 41 and a first mounting frame 42 fixed on the left side wall inside the packaging ring 26. A group of insulating cylinders 44 are hingedly assembled on the front and rear sides of the inner wall of the first bracket 41 through a movable shaft 43. A positive platinum electrode rod 45 is fixedly assembled inside the group of insulating cylinders 44. The negative electrode unit 50 includes a second bracket 51 and a second mounting frame 52 fixed on the right side wall inside the packaging ring 26. Another group of insulating cylinders 44 are hingedly assembled on the front and rear sides of the inner wall of the second bracket 51 through a movable shaft 43. A negative platinum electrode rod 55 is fixedly assembled inside the group of insulating cylinders 44. The positive platinum electrode rod 45 is connected to the conductive wire 37 on the positive branch line 35, and the negative platinum electrode rod 55 is connected to the conductive wire 37 on the negative branch line 36. The positive platinum electrode rod 45 and the negative platinum electrode rod 55 are of the same length and extend into the culture tube 22 together.

[0032] During actual use, the positive electrode 31 is energized, which can cause the positive electrode platinum electrode rod 45 to be energized along the main positive electrode line 33, the positive branch line 35 and the conductive line 37, and then an electric field is generated between the positive electrode platinum electrode rod 45 and the negative electrode platinum electrode rod 55, providing electrical stimulation to the cells in the culture tubes 22. Since all the culture tubes 22 are independent of the culture plate 21, and the stimulation electrode array unit 30 is energized to make the voltage in each group of culture tubes 22 the same, the electrical stimulation obtained by each group of culture tubes 22 is the same, so that the cells or tissues on the culture plate unit 20 are electrically stimulated indiscriminately, which makes it more convenient to record the cell status in each group of culture tubes 22.

[0033] It is worth noting that a multi-channel field stimulation electrode array system disclosed in this embodiment also includes an incubator 10, and a shelf 14 for supporting and limiting the culture plate unit 20 is arranged on the inner wall of the incubator 10, and the number of the shelf 14 is the same as the number of the culture plate units 20, which is convenient for quickly placing the culture plate units 20 on the shelf 14, and a power plug 15 matching the power connector 25 is fixed on the inner wall of the incubator 10, which is convenient for the power plug 15 and the power connector 25 to be quickly plugged in, thereby prompting the group of culture plate units 20 to be powered on synchronously as a whole, and a box door 11 is hingedly assembled on the front side wall of the incubator 10, and an electric control box 12 is fixed on the top of the incubator 10, and a display screen 13 matching the culture plate unit 20 is installed on the front side wall of the electric control box 12, and the display screen 13 can display the independent stimulation response of the culture tubes 22 inside the culture plate unit 20, and can achieve independent display of each group of culture tubes 22, with better visualization effect.

[0034] The electrical control box 12 is equipped with a programmable multi-channel microcurrent stimulator, including a stimulation chip, a signal transmission module and a power supply module, wherein: the stimulation chip is connected to the signal transmission module, the signal transmission module transmits the electrical stimulation signal generated after the external device encodes the image information to the stimulation chip, the stimulation chip is connected to the positive platinum electrode rod 45 and the negative platinum electrode rod 55, and transmits the control signal to the positive platinum electrode rod 45 and the negative platinum electrode rod 55 respectively, the power supply module includes a multi-channel voltage source module and a multi-channel voltage amplifier, the multi-channel voltage source module is used to generate a specific frequency and pulse width according to the control signal, each channel can be set separately, and the multi-channel voltage amplifier is used to amplify the voltage signal generated by the voltage source to ensure that the intensity and shape of the voltage meet the specific stimulation requirements.

[0035] Second embodiment: This embodiment discloses Figures 1 to 9A multi-channel field stimulation electrode array system is shown, which includes all the technical features disclosed in the first embodiment, and at the same time, a first pushing member 46 is fixed on the left inner wall of the first mounting frame 42, a first limiting member 47 is fixed on the right inner wall of the first mounting frame 42, and the other side of the first pushing member 46 and the first limiting member 47 are fixedly mounted on the outer wall of the positive platinum electrode rod 45, a second pushing member 53 is fixed on the right inner wall of the second mounting frame 52, a second limiting member 54 is fixed on the left inner wall of the second mounting frame 52, and the other side of the second pushing member 53 and the second limiting member 54 are fixedly mounted on the outer wall of the negative platinum electrode rod 55.

[0036] During actual use, the positive platinum electrode rod 45 can rotate relative to the movable shaft 43. Similarly, the negative platinum electrode rod 55 can also rotate equivalent to the movable shaft 43. At the same time, under the clamping action of the first pushing member 46 and the first limiting member 47, the positive platinum electrode rod 45 can be limited and fixed to prevent the positive platinum electrode rod 45 from rotating on its own. Similarly, the second pushing member 53 and the second limiting member 54 can limit and fix the negative platinum electrode rod 55 to prevent the negative platinum electrode rod 55 from rotating on its own. This can ensure that the distance between the positive platinum electrode rod 45 and the negative platinum electrode rod 55 is relatively fixed, thereby ensuring that the electrical stimulation field strength in each group of culture tubes 22 is the same.

[0037] It should be noted that the first pushing member 46, the first limiting member 47, the second pushing member 53, and the second limiting member 54 are all pressure-bearing air bags, and the first pushing member 46, the first limiting member 47, the second pushing member 53, and the second limiting member 54 are respectively connected to the air pump through the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe, and the first solenoid valve, the second solenoid valve, the third solenoid valve, and the fourth solenoid valve are respectively fixedly assembled on the first air pipe, the second air pipe, the third air pipe, and the fourth air pipe.

[0038] Before the cultivation work, the number of culture tubes 22 can be customized according to the cultivation needs of the culture plate unit 20, which is convenient for the horizontal comparison of different cells during cultivation and improves the detection accuracy of cells under stress stimulation. During the cultivation work, the culture plate unit 20 has an electrode array with multiple independent channels, each channel can be independently controlled to achieve multi-channel point stimulation and improve the flux, and an electrical stimulation field is generated inside the culture tube 22, which does not interfere with each other and has strong independence.

[0039] It is worth noting that, due to the need to culture different cells on the same group of culture plates 21, the stimulation electrode array unit 30 is required to have the ability to adjust the current so that different electrical stimulation field intensities can be generated in different culture tubes 22. Therefore, a group of electrically controlled sliding rheostats are connected in series between each group of positive branches 35 and the conductive wires 37. In this way, the current value generated in the corresponding culture tube 22 can be changed by remotely controlling the tissue in the circuit connected to the sliding rheostat, and then the electrical stimulation field strength inside the group of culture tubes 22 can be changed, thereby satisfying the device's regulatory needs for providing different electrical stimulations when culturing different cells or tissues.

[0040] The positive platinum electrode rod 45 and the negative platinum electrode rod 55 use platinum electrodes, which have the advantages of high conductivity, non-toxicity, low impedance, high stability, and good biocompatibility. Under normal use, the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are both vertically downward and immersed in the cell fluid inside the culture tube 22, and then the electric stimulation field generated between the positive platinum electrode rod 45 and the negative platinum electrode rod 55 is used to stimulate the cells.

[0041] Furthermore, when the platinum electrodes are used, since the positions of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are relatively fixed, the distribution of the electrical stimulation field generated between the two is also fixed. In order to detect the stimulation effect on cells when the platinum electrodes generate different electrical stimulation fields, in actual use, a control signal can be sent to the air pump through the stimulation chip in the electrical control box, and the positive electrode unit 40 and the negative electrode unit 50 can be controlled by the cooperation of the air pump and the solenoid valve, so as to facilitate the adjustment of the relative position between the positive platinum electrode rod 45 and the negative platinum electrode rod 55, thereby changing the distribution form of the electrical stimulation field in the culture tube 22, and giving cells or tissues more electrical stimulation, specifically: When the second solenoid valve and the third solenoid valve are controlled to open at the same time, the air pump will introduce gas into the first limiter 47 and the second pusher 53 through the second air pipe and the third air pipe respectively, so that the first limiter 47 and the second pusher 53 are inflated synchronously, and then the first limiter 47 pushes the insulating tube 44 on the top of the positive platinum electrode rod 45 to squeeze the first pusher 46, so that the positive platinum electrode rod 45 starts to rotate clockwise around the movable shaft 43. Similarly, the second pusher 53 pushes the insulating tube 44 on the top of the negative platinum electrode rod 55 to squeeze the first pusher 46. The cylinder 44 squeezes the second limit piece 54, so that the negative platinum electrode rod 55 starts to rotate synchronously clockwise around the movable axis 43, so that the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 will gradually increase but remain in a parallel state. At this time, since the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 increases, the intensity of the electrical stimulation field between the platinum electrodes will gradually weaken when the current remains unchanged, so as to detect the stress response of the cultured cells after the intensity of the electrical stimulation field is reduced.

[0042] It is worth mentioning that when gas is introduced into the first pushing member 46 and the second limiting member 54 simultaneously, the positive platinum electrode rod 45 and the negative platinum electrode rod 55 will be synchronously rotated counterclockwise around the movable axis 43 respectively, which will also reduce the intensity of the electrical stimulation field generated by the platinum electrode, which is consistent with the effect achieved by the above operation.

[0043] When the air pump synchronously introduces gas into the first pusher 46 and the second pusher 53 to expand them, the first pusher 46 pushes the positive platinum electrode rod 45 to rotate counterclockwise around the movable axis 43, while the second pusher 53 pushes the negative platinum electrode rod 55 to rotate clockwise, so that the distance between the ends of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 is reduced, thereby enhancing the strength of the electrical stimulation field between the platinum electrodes and increasing the stress stimulation to the cultured cells, so as to detect the stress response of the cultured cells after the strength of the electrical stimulation field is enhanced.

[0044] However, in actual use, after long-term use, the surface of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 will be oxidized and passivated due to organic matter in the cell fluid, which will affect the response slope of the platinum electrode. The most intuitive thing is that the resistance value of the platinum electrode increases, and then the current value in the circuit decreases, and the intensity of the electric stimulation field formed is reduced, which makes the response speed of the platinum electrode significantly slower, resulting in a decrease in the response of the cell to stress stimulation. The response of the cell to stress stimulation can be detected by the electric marking measurement technology or the cursor measurement technology. The current change of the cell can be detected. Therefore, the passivation degree of the positive platinum electrode rod 45 and the negative platinum electrode rod 55 can be reflected by detecting the change in the current value in each group of culture test tubes 22, so as to facilitate real-time adjustment measures. Specifically: When the current in a group of culture tubes 22 on the display screen 13 decreases by more than the error threshold, and the supply voltage of the power plug 15 does not change, it can be concluded that the platinum electrodes inside the group of culture tubes 22 have been significantly passivated. At this time, the stimulation chip in the electrical control box 12 is used to control the reduction of the resistance value of the sliding rheostat in the electrode array circuit corresponding to the group of culture tubes 22, thereby increasing the current value in the electrode array circuit, so that the current value increases to the same as the current value in other groups of electrode arrays, thereby increasing the electrical stimulation field generated by the passivated platinum electrodes.

[0045] When the current in a group of culture test tubes 22 on the display screen 13 decreases by more than the maximum threshold, it indicates that the positive platinum electrode rod 45 and the negative platinum electrode rod 55 are severely passivated and an oxide film is formed. This not only seriously reduces the intensity of the electric stimulation field generated by the platinum electrode, but also adsorbates on the platinum electrode, which greatly increases the resistance of the platinum electrode after it is connected to the circuit, seriously affecting the control accuracy of the electric stimulation field during cell culture, and is also not conducive to the detection of cell culture. At this time, the group of positive platinum electrode rods 45 and negative platinum electrode rods 55 are taken out for soaking, washing and cleaning.

[0046] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A multi-channel field stimulation electrode array system, characterized in that: The culture plate unit (20) comprises a culture plate (21) and a cover plate (24); culture tubes (22) are evenly arranged on the culture plate (21); a stimulation electrode array unit (30) is installed in the cover plate (24); a positive electrode unit (40) and a negative electrode unit (50) corresponding to the culture tubes (22) are fixed to the bottom of the cover plate (24); and the positive electrode unit (40), the negative electrode unit (50) and the stimulation electrode array unit (30) are connected; The stimulation electrode array unit (30) comprises a positive electrode (31) and a negative electrode (32) fixed on the left side wall of the cover plate (24); the positive electrode (31) is connected to a main positive electrode line (33) laid inside the cover plate (24); the negative electrode (32) is connected to a main negative electrode line (34) laid inside the cover plate (24); the main positive electrode line (33) is connected to positive electrode branches (35) having the same number of columns as the culture test tubes (22); the main negative electrode line (34) is connected to negative electrode branches (36) having the same number of columns as the culture test tubes (22); each group of positive electrode units (40) is connected to the positive electrode branches (35) via a conductive line (37); and each group of negative electrode units (50) is connected to the negative electrode branches (36) via a conductive line (37).

2. The multi-channel field stimulation electrode array system according to claim 1, characterized in that: The culture plate unit (20) further comprises a liquid adding port (23) provided on the surface of the culture plate (21) and corresponding one-to-one with the culture test tube (22); a power connector (25) is fixed in the middle of the left side of the cover plate (24); the power connector (25) is connected to the positive electrode (31) and the negative electrode (32); and a receiving groove (27) corresponding to the positive electrode unit (40) and the negative electrode unit (50) is provided at the bottom of the cover plate (24).

3. The multi-channel field stimulation electrode array system according to claim 2, characterized in that: A packaging ring (26) corresponding one to one with the culture test tube (22) is fixed at the bottom of the cover plate (24); the positive electrode unit (40) and the negative electrode unit (50) are assembled inside the packaging ring (26); the positive electrode unit (40) and the negative electrode unit (50) have the same composition structure and are arranged relative to each other; the packaging ring (26) matches the liquid adding port (23).

4. The multi-channel field stimulation electrode array system according to claim 3, characterized in that: The positive electrode unit (40) comprises a first bracket (41) and a first mounting frame (42) fixed to the inner left wall of the packaging ring (26); a group of insulating cylinders (44) are hingedly mounted on the front and rear sides of the inner wall of the first bracket (41) via a movable shaft (43); a positive platinum electrode rod (45) is fixedly mounted inside the group of insulating cylinders (44); A first pushing member (46) is fixed on the left inner wall of the first mounting frame (42), a first limiting member (47) is fixed on the right inner wall of the first mounting frame (42), and the other sides of the first pushing member (46) and the first limiting member (47) are fixedly mounted on the outer wall of the positive platinum electrode rod (45).

5. The multi-channel field stimulation electrode array system according to claim 4, characterized in that: The negative electrode unit (50) comprises a second bracket (51) and a second mounting frame (52) fixed to the right inner wall of the packaging ring (26); another set of insulating cylinders (44) are hingedly mounted on the front and rear sides of the inner wall of the second bracket (51) via a movable shaft (43); a negative platinum electrode rod (55) is fixedly mounted inside the set of insulating cylinders (44); A second pushing member (53) is fixed on the right inner wall of the second mounting frame (52), a second limiting member (54) is fixed on the left inner wall of the second mounting frame (52), and the other sides of the second pushing member (53) and the second limiting member (54) are fixedly mounted on the outer side wall of the negative platinum electrode rod (55).

6. The multi-channel field stimulation electrode array system according to claim 5, characterized in that: The first pushing member (46), the first limiting member (47), the second pushing member (53) and the second limiting member (54) are all pressure-bearing air bags, and the first pushing member (46), the first limiting member (47), the second pushing member (53) and the second limiting member (54) are respectively connected to the air pump through the first air pipe, the second air pipe, the third air pipe and the fourth air pipe, and the first air pipe, the second air pipe, the third air pipe and the fourth air pipe are respectively fixedly equipped with a first solenoid valve, a second solenoid valve, a third solenoid valve and a fourth solenoid valve.

7. The multi-channel field stimulation electrode array system according to claim 6, characterized in that: The positive platinum electrode rod (45) is connected to the conductive wire (37) on the positive branch line (35), and the negative platinum electrode rod (55) is connected to the conductive wire (37) on the negative branch line (36). The positive platinum electrode rod (45) and the negative platinum electrode rod (55) have the same length and extend into the culture tube (22) together.

8. The multi-channel field stimulation electrode array system according to claim 2, characterized in that: It also comprises an incubator (10), wherein a shelf (14) for supporting and limiting the position of the culture plate units (20) is arranged on the inner side wall of the incubator (10), and the number of the shelf (14) is the same as the number of the culture plate units (20), and a power plug (15) matching the power connector (25) is fixed on the inner side wall of the incubator (10).

9. The multi-channel field stimulation electrode array system according to claim 8, characterized in that: The incubator (10) has a door (11) hingedly mounted on the front side wall, an electric control box (12) is fixed on the top of the incubator (10), and a display screen (13) matching the culture plate unit (20) is mounted on the front side wall of the electric control box (12).

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