Recombinant strain of high-yield PBAT degrading enzyme as well as construction method and application of recombinant strain

Abstract

The invention belongs to the technical field of gene engineering, and particularly provides a recombinant strain of high-yield PBAT degrading enzyme, which is constructed by integrating an ERO1 gene onto a pichia pastoris genome through an expression vector. The invention also provides a method for producing PBAT degrading enzyme by fermentation based on the recombinant strain. Compared with the existing genetic engineering strain Pichia pastoris X33 / IsPETase, the recombinant strain of the high-yield PBAT degrading enzyme provided by the invention has the advantage that the expression quantity of the enzyme during fermentation is increased by 3.29 times. The recombinant strain provided by the invention can be used for industrial fermentation production of IsPETase, and the produced IsPETase can catalytically degrade plastic PBAT under an alkaline condition, and can be applied to the industries of plastic synthesis, plastic garbage degradation and the like, so that the environmental burden is reduced.

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