Reagent transfer equipment, mass spectrometer and use method of mass spectrometer

Abstract

The invention relates to a reagent transfer device and a mass spectrometer, and the device comprises a box body which forms a space for the installation of a structure of the reagent transfer device; the cup arranging device is used for arranging the reaction cups; the magnetic bead reagent bin is arranged in the box body and is arranged on one side of the cup arranging device; the medicament transporting device is arranged on one side, far away from the magnetic bead reagent bin, of the cup arranging device, and the medicament transporting device comprises a plurality of liquid supply pumps arranged on one side of the cup arranging device, a medicament uniformly-mixing assembly connected with the liquid supply pumps and a connecting pipe arranged between the liquid supply pumps and the medicament uniformly-mixing assembly; the transition assembly is arranged on one side of the magnetic bead reagent bin and the reagent conveying device, and the transition assembly comprises a moving rail arranged in the box body and a transition trolley running on the moving rail; the mechanical arm assemblies are arranged at the top of the box body, and the multiple mechanical arm assemblies are arranged; the mass spectrometer is arranged on one side, close to the magnetic bead reagent bin, of the box body and is used for detecting the operated reagent. The detection cost can be reduced.

Description

Technical Field

[0001] The present application relates to the field of biology, and in particular to a reagent transport device and a mass spectrometer and a method for using the same. Background Art

[0002] Mass spectrometry is a spectroscopic method parallel to spectroscopy. In general, it refers to a specialized technology widely used in various disciplines to identify compounds by preparing, separating, and detecting gas-phase ions. Mass spectrometry can provide rich structural information in one analysis. Combining separation technology with mass spectrometry is a breakthrough in separation science. Among the many analytical testing methods, mass spectrometry is considered to be a universal method that has both high specificity and high sensitivity and has been widely used.

[0003] At present, when performing mass spectrometry analysis on samples, it can be roughly divided into four steps: sample collection, sample pretreatment, analytical testing, and data processing and reporting results. During the entire analysis process, the sample needs to be processed in multiple steps, such as adding detection reagents, adding cleaning agents, and adding magnetic beads for separation of processed samples.

[0004] At present, there is an automated mass spectrometry detection device, including an equipment casing and a mounting table installed in the equipment casing, a sample turntable, a transfer gripper, a sample track, a capping device, a sample loading component, a purification component and a rotating plate group; the sample turntable is set on the mounting table, and a reagent trough, a needle rack and a reagent rack are sequentially arranged on its side; a sample track is set on the side of the sample turntable. Although this device solves the problem of automated detection, it needs to establish an assembly line to process the samples, resulting in a large footprint of the equipment, thereby increasing the cost required for detection. Summary of the invention

[0005] In order to reduce detection costs, the present application provides a reagent transport device and a mass spectrometer.

[0006] On the one hand, the present application provides a reagent transport device and a mass spectrometer using the following technical solutions: A reagent transport device and a mass spectrometer, comprising: The box body forms a space for the structure of the reagent transfer equipment to be installed; Cup sorter, used to arrange the reaction cups; A magnetic bead reagent chamber is arranged inside the box body and on one side of the cup organizer, and is used to hold the magnetic beads required to be placed in the reaction cups; A medicine transport device is arranged on a side of the cup sorter away from the magnetic bead reagent compartment, the medicine transport device comprises a plurality of liquid supply pumps arranged on one side of the cup sorter, a medicine mixing assembly connected to the liquid supply pumps, and a connecting pipe arranged between the liquid supply pumps and the medicine mixing assembly; A transition component is arranged on one side of the magnetic bead reagent compartment and the drug transport device, and the transition component includes a moving rail arranged in the box body and a transition trolley traveling on the moving rail; A mechanical arm assembly is arranged on the top of the box body, and a plurality of the mechanical arm assemblies are arranged; A mass spectrometer is arranged on a side of the box body close to the magnetic bead reagent compartment, and is used to detect the reagent after operation.

[0007] By adopting the above technical solution, when the reaction cup needs to be processed, multiple reaction cups are arranged by the cup sorter in the box, the reaction cup is placed at the drug transport device by the mechanical arm assembly, the internal standard liquid is added by the liquid supply pump, and then the reaction cup is placed on the transition trolley by the mechanical arm assembly, and the magnetic beads are added to the reaction cup, and then the reaction cup is placed at the drug transport device by the mechanical arm assembly, and the drug mixing assembly is used to oscillate, and finally transported to the mass spectrometer for detection. During use, the mechanical arm assembly will travel back and forth between the drug transport device, the transition trolley, and the magnetic bead reagent bin, and the drug transport device, the transition trolley, and the magnetic bead reagent bin are arranged around the cup sorter, saving space for the reagent transport equipment, and the transition trolley can drive the reaction cup to move in a straight line, so that the reaction cup can be loaded, tested, and other operations, further saving space for the reagent transport equipment, so that there is no need to establish an assembly line to process the sample, reducing the cost required for detection.

[0008] Optionally, the transition trolley includes a magnetic seat installed on the moving rail and a cup seat embedded in the magnetic seat, a magnet groove is opened along the width direction of the magnetic seat, a cup groove is opened on the cup seat, the positions of the cup groove and the magnet groove correspond to each other, a magnet is embedded in the magnet groove, a fixing part for fixing the magnet is embedded on the magnetic seat, and a control part for controlling the lifting and lowering of the magnet is provided at the bottom of the magnet groove.

[0009] By adopting the above technical solution, during operation, the reaction cup is placed on the cup groove of the transition trolley through the robotic arm assembly, and the magnetic beads in the magnetic bead reagent chamber are placed in the reaction cup. The magnet is controlled to rise through the control component so that it can sense the magnetic beads in the reaction cup, thereby adsorbing the substances that do not need to be detected in the reaction cup and retaining the required supernatant.

[0010] Optionally, the cross-section of the transition trolley is convex-shaped, the magnetic seat is arranged on both sides of the cup seat and is lower than the cup seat, the length of the magnetic seat is smaller than the length of the cup seat, and the number of the magnet slots is at least one less than the number of the cup slots.

[0011] By adopting the above technical solution, the transition trolley can place the reaction cup that needs to be magnetically attracted, and can also place the supernatant after adding the diluent. The test tube cup for placing the supernatant does not need to be magnetically attracted, and can be used for the position transition of the reagent, adapting to the positive detection and negative detection in this detection. The height setting of the magnetic suction seat makes the magnetic suction point close to the bottom of the reaction cup, so that the supernatant required for detection can be better sucked out.

[0012] Optionally, the robotic arm assembly includes a column, a slide rail perpendicular to and slidably connected to the column, a slider slidably connected to the slide rail, and a grabbing arm rotatably connected to the slider, the grabbing arm can grab a reaction cup and move the reaction cup to the drug mixing assembly or the cup holder, and the slider is connected to a driving member.

[0013] By adopting the above technical solution, the setting of the robot arm assembly can be used to move the reaction cup. When the reaction cup needs to be moved, the driving member is started, so that the driving member drives the slider to slide in the slide rail, thereby moving the grasping arm to above the position where the reaction cup is located, and the grasping arm is rotated so that the inner wall of the grasping arm abuts against the outer wall of the reaction cup, and then the reaction cup is lifted by rotating the grasping arm, and then the driving member is started, and the slider drives the reaction cup to the desired position, and then the reaction cup is put down. The up and down displacement of the grasping arm needs to be achieved by the movement of the slide rail relative to the column.

[0014] Optionally, the medicine mixing assembly includes a mounting plate arranged on the side of the cup sorter away from the magnetic bead reagent chamber, a driving motor arranged in the mounting plate, a rotating plate connected to the output shaft of the driving motor, and a track plate arranged on the outside of the rotating plate, and the driving motor can drive the rotating plate to rotate eccentrically on the track plate.

[0015] By adopting the above technical solution, when the substance in the reaction cup needs to be shaken, the drive motor is started, and the output shaft of the drive motor drives the rotating disk to make eccentric movement along the range formed by the inner wall of the track disk. The setting of the track disk not only limits the rotation range of the rotating disk, but also reduces the amplitude of mixing, avoids the reagent from splashing out of the reaction cup, and avoids the deviation of the test data. The drive motor is installed in the installation disk, which further saves the space required for the entire device, thereby further saving the detection cost.

[0016] Optionally, a placement groove for placing a reaction cup is formed at one end of the rotating disk, and a mounting groove is formed at the other end of the rotating disk. An eccentric rod is fixedly placed in the mounting groove, and the driving shaft of the driving motor is connected to the eccentric rod. A rotating rod is inserted in the center of the track disk, and the rotating rod is also passed through the rotating disk. The rotating disk can make eccentric motion around the rotating rod, and the driving motor is arranged at the bottom of the track disk.

[0017] By adopting the above technical solution, when the driving motor is started, the output shaft of the driving motor is connected to the eccentric rod, so that the output shaft can drive the eccentric rod to rotate, thereby driving the rotating disk to rotate. At this time, a rotating rod inserted in the center of the rotating disk is also provided. The rotating rod further limits the rotation range of the rotating disk, so that the rotating disk can perform eccentric movement around the rotating rod. When the rotating disk rotates, the rotation trajectory of the rotating disk around the rotating rod is in an 8-shape, making the mixing more uniform.

[0018] Optionally, a magnetic attraction device is further provided at the bottom of the track plate, and the magnetic attraction device includes a magnet arranged on the placement slot and a lifting assembly arranged under the magnet, and the lifting assembly is used to drive the magnet to rise or fall along the axial direction of the placement slot.

[0019] By adopting the above technical solution, after the cleaning liquid is added to the reaction cup, the lifting assembly drives the magnet to move away from the reaction cup, and the attraction between the magnet and the magnetic beads continues to decrease. At this time, the oscillation device drives the reaction cup to shake, so that the magnetic beads and the cleaning liquid are fully mixed. In this way, the magnetic beads can be fully mixed with the cleaning liquid during the magnetic bead cleaning process to reduce the residual non-target substances on the magnetic beads and increase the concentration of the target substances.

[0020] On the other hand, the present application also provides a reagent transport device and a method for using a mass spectrometer, which adopts the following technical solution: Optional, include the following steps: S1. Place the reaction cup: Place the reaction cup in the cup sorter, and place the cup sorter on the transition trolley through the gripper arm; S2, adding internal standard: move the reaction cup to the reagent mixing component through the gripping arm, and add the internal standard liquid through the liquid supply pump; S3, sample loading: the grab arm goes to the magnetic bead reagent compartment to absorb the sample, goes to the transition trolley, and injects the reagent into the reaction cup; S4, add magnetic beads: the grab arm goes to the magnetic bead reagent compartment to absorb the magnetic bead liquid, goes to the transition trolley, and injects the reagent into the reaction cup; S5, vortex oscillation: the gripper arm places the reaction cup in the placement tank, and mixes it through the drug mixing component; S6, magnetic attraction: Move the reaction cup to the cup slot of the transition trolley again through the gripper arm, and use the magnet in the magnet slot to magnetically attract the magnetic beads in the reaction cup for 55s-1min5s; S7, adding diluent: add the diluent in the magnetic bead reagent compartment into the reaction cup on the transition cart, and absorb the supernatant through a pipette; S8. Transferring the supernatant and detecting: The supernatant extracted from the reaction cup is moved to the sample cup through the gripper arm, and the sample cup is sent to the mass spectrometer for detection.

[0021] By adopting the above technical solution, during operation, the reaction cup is first placed in the cup sorter, and the reaction cups are sorted and arranged by the cup sorter, and then placed on the transition trolley by the grab arm, so as to facilitate the subsequent addition of internal standards and sample loading. Then, the magnetic beads need to be added to the reaction cup by the mechanical arm assembly, and then the reaction cup is moved to the reagent mixing assembly for mixing. After mixing, different magnetic suction methods can be used for forward and reverse detection according to different detection requirements. Finally, the detection liquid is obtained by adding the diluent and taking the supernatant, and sent to the mass spectrometer for detection.

[0022] Optionally, mixing is performed through a medicine mixing assembly, including the steps of: starting a drive motor, wherein the output shaft of the drive motor drives an eccentric rod to rotate, thereby driving a rotating disk to rotate, and at this time, the rotating disk performs an eccentric motion around the rotating rod.

[0023] By adopting the above technical solution, the liquid in the reaction cup needs to be mixed before magnetic attraction. The specific operation is: turn on the drive motor, and the output shaft of the drive motor can drive the eccentric shaft to start driving the rotating disk to perform eccentric movement around the rotating rod, thereby shaking the test liquid and finally achieving uniformity.

[0024] Optionally, the magnetic beads in the reaction cup are magnetically attracted for 55s-1min5s by the magnet in the magnet slot, including the steps of: placing the reaction cup on the cup slot of the transition trolley through the robotic arm assembly, placing the magnetic beads in the magnetic bead reagent compartment into the reaction cup, controlling the magnet to rise by the control component, and the magnet and the magnetic beads in the reaction cup are sensed to adsorb the substances in the reaction cup that do not need to be detected.

[0025] By adopting the above technical solution, during the magnetic attraction process, the reaction cup is first placed on the transition trolley through the robotic arm assembly, and the rise of the magnet is controlled by the control component, so that the magnet attracts the magnetic beads in the reaction cup, thereby absorbing the liquid to be tested or the liquid that does not need to be tested in the reaction cup, which is convenient for subsequent detection.

[0026] In summary, the present application includes at least one of the following beneficial technical effects: By setting up a box, a cup sorter, a magnetic bead reagent bin, a drug transport device, a liquid supply pump, a drug mixing component, a connecting pipe, a transition component, a motion track, a transition trolley, a robotic arm component and a mass spectrometer, when the reaction cup needs to be processed, multiple reaction cups are arranged by the cup sorter in the box, so that the reaction cups can be transported to other components in sequence by the robotic arm, and then the reaction cup is placed on the drug transport device by the robotic arm component, and the internal standard liquid is added by the liquid supply pump, and then the reaction cup is placed on the transition trolley by the robotic arm component, and then the magnetic beads are added to the reaction cup, and then the reaction cup is placed on the drug transport device by the robotic arm component, and it is shaken by the drug mixing component, and after magnetic absorption, a diluent is added and the supernatant is taken out after standing, and finally it is transported to the mass spectrometer for detection by the robotic arm component. During use, the robotic arm assembly will go back and forth between the medicine transport device, the transition trolley and the magnetic bead reagent bin, and the medicine transport device, the transition trolley and the magnetic bead reagent bin are arranged around the cup sorter, saving the space of the reagent transport device, and the transition trolley can drive the reaction cup to move in a straight line, so that the reaction cup can perform operations such as sample loading and testing, further saving the space of the reagent transport device, so that there is no need to set up an assembly line to process the samples, reducing the cost required for testing; By setting up a hopper, a slide, a conveying mechanism, a transfer channel, a reaction cup holder, a lifting mechanism, and a baffle, when in use, after placing the reaction cup in the hopper, under the action of the slope, the reaction cup slides out from the discharge port and falls on the slide. Since a cup brim is formed on the top of the reaction cup, the reaction cup can be stuck on the slide, and under the action of gravity, it slides to the bottom of the slide, and the conveying mechanism transfers the reaction cup to the reaction cup holder through the transfer channel. In this way, the automatic stacking and transfer of the reaction cups is realized. When the top of the reaction cup slides out of the discharge port first, at this time, the reaction cup cannot fall into the slide, and the lifting mechanism drives the baffle to rise and push the reaction cup back. Under the action of the slope, the reaction cup changes direction and falls smoothly into the slide; By setting up a magnetic suction seat, a cup seat, a magnet slot, a cup slot, a magnet, a fixing part and a control part, during operation, the reaction cup is placed on the cup slot of the transition trolley through the robotic arm assembly, and the magnetic beads in the magnetic bead reagent compartment are placed into the reaction cup. The magnet is controlled to rise through the control part so that it can sense the magnetic beads in the reaction cup, thereby adsorbing the substances that do not need to be detected in the reaction cup and retaining the required supernatant. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of this application.

[0028] Figure 2 It is a schematic diagram of the overall structure of this application from another perspective.

[0029] Figure 3It is a schematic diagram of the overall structure of the cup organizer of the present invention.

[0030] Figure 4 It is a schematic diagram of the overall structure of the cup organizer of the present invention from another perspective.

[0031] Figure 5 It is a schematic diagram of the overall structure of the transition component of this application.

[0032] Figure 6 It is a schematic diagram of the overall structure of the robotic arm assembly of the present application.

[0033] Figure 7 It is a schematic diagram of the overall structure of the robotic arm assembly of the present application from another perspective.

[0034] Figure 8 It is a schematic diagram of the overall structure of the medicine transport device of the present application.

[0035] Fig. 9 It is a schematic diagram of the overall structure of the medicine mixing component of the present application.

[0036] Explanation of the reference numerals in the accompanying drawings: 1. housing; 2. cup sorter; 21. hopper; 22. slide; 23. reaction cup holder; 24. baffle; 3. magnetic bead reagent compartment; 4. drug transport device; 41. liquid supply pump; 42. drug mixing assembly; 421. mounting plate; 422. drive motor; 423. rotating plate; 424. track plate; 425. eccentric rod; 426. rotating rod; 5. placement slot; 6. mounting slot; 7. connecting pipe; 8. transition assembly; 81. moving rail; 82. transition trolley; 821. magnetic seat; 822. cup holder; 824. fixing part; 9. magnet slot; 10. cup slot; 11. robot arm assembly; 111. column; 112. slide rail; 113. slider; 114. grab arm; 12. driving part; 13. magnetic device; 131. magnet; 14. mass spectrometer. DETAILED DESCRIPTION

[0037] The following is combined with Figure 1-9 This application is described in further detail.

[0038] On the one hand, embodiments of the present application disclose a reagent transport device and a mass spectrometer.

[0039] Reference Figure 1 and Figure 2A reagent transport device and a mass spectrometer, comprising a box body 1 forming a space for the structure of the reagent transport device to be installed, a cup sorter 2 arranged in the box body 1 for arranging reaction cups, a magnetic bead reagent compartment 3 arranged inside the box body 1 and on one side of the cup sorter 2, a drug transport device 4 arranged on the side of the cup sorter 2 away from the magnetic bead reagent compartment 3, a transition component 8 arranged on the inner side of the drug transport device 4 and the magnetic bead reagent compartment 3, a mechanical arm component 11 arranged on the top of the inner wall of the box body 1, and a mass spectrometer 14 arranged on the outer side of the box body 1 close to the installation of the magnetic bead reagent compartment 3.

[0040] The magnetic bead reagent compartment 3 is used to hold the magnetic beads and reagents required to be placed in the reaction cup, so as to facilitate the mechanical arm assembly 11 to add the magnetic beads and reagents; the drug transport device 4 includes a plurality of liquid supply pumps 41 arranged on one side of the cup sorter 2, a drug mixing assembly 42 connected to the liquid supply pump 41, and a connecting pipe 7 arranged between the liquid supply pump 41 and the drug mixing assembly 42; the transition assembly 8 includes a moving rail 81 arranged in the box body 1 and a transition trolley 82 running on the moving rail 81; there are multiple mechanical arm assemblies 11, and different substances can be transported or added through different mechanical arm assemblies 11; the mass spectrometer 14 is used to detect the reagents after the operation.

[0041] When the reaction cup needs to be processed, multiple reaction cup machines are arranged by the cup sorter 2 in the box 1, so that the reaction cups can be transported to other components in sequence by the robot arm, and then the reaction cup is placed on the drug transport device 4 by the robot arm component 11, and the internal standard liquid is added by the liquid supply pump 41, and then the reaction cup is placed on the transition trolley 82 by the robot arm component 11, and then the magnetic beads are added to the reaction cup, and then the reaction cup is placed on the drug transport device 4 by the robot arm component 11, and it is shaken by the drug mixing component 42. After magnetic attraction, the diluent is added and the supernatant is taken after standing, and finally it is transported to the mass spectrometer 14 for detection by the robot arm component 11.

[0042] During use, the robotic arm assembly 11 will move back and forth between the medicine transport device 4, the transition trolley 82 and the magnetic bead reagent compartment 3, and the medicine transport device 4, the transition trolley 82 and the magnetic bead reagent compartment 3 are arranged around the cup sorter 2, saving space for the reagent transport equipment, and the transition trolley 82 can drive the reaction cup to move in a straight line, so that the reaction cup can be loaded with samples, tested and other operations, further saving space for the reagent transport equipment, so that there is no need to establish an assembly line to process the samples, thereby reducing the cost required for testing.

[0043] Reference Figure 3 and Figure 4The cup sorter 2 includes a hopper 21, a slide 22, a conveying mechanism (not shown in the figure) arranged at the bottom of the slide 22, and a transfer channel (not shown in the figure) arranged at the bottom of the slide 22. A reaction cup holder 23 is also arranged on the side of the slide 22 away from the hopper 21. A slope is formed inside the hopper 21, and a discharge port is opened on the hopper 21. The slide 22 is arranged below the discharge port to receive the reaction cups poured out of the discharge port. When in use, after the reaction cup is placed in the hopper 21, the reaction cup slides out of the discharge port under the action of the slope and falls on the slide 22. Since a cup brim is formed on the top of the reaction cup, the reaction cup can be stuck on the slide 22, and under the action of gravity, it slides to the bottom of the slide 22, and the conveying mechanism transfers the reaction cup to the reaction cup holder 23 through the transfer channel. In this way, the automatic stacking and transfer of the reaction cups are realized.

[0044] The cup sorter 2 also includes a lifting mechanism (not shown in the figure) and a baffle 24. The baffle 24 is vertically arranged on the hopper 21. The lifting mechanism drives the baffle 24 to be lifted and lowered. The baffle 24 is arranged near the discharge port. When the top of the reaction cup slides out of the discharge port first, the reaction cup cannot fall into the slide 22. The lifting mechanism drives the baffle 24 to rise and push the reaction cup back. Under the action of the slope, the reaction cup changes direction and falls smoothly into the slide 22.

[0045] Of course, a plurality of sensors are installed on the cup sorter 2 to ensure the normal and continuous operation. In this embodiment, no specific description is given. The conveying mechanism and the lifting mechanism are both mechanisms known to those skilled in the art and only need to meet the requirements. In this embodiment, no specific description or limitation is given to the conveying mechanism and the lifting mechanism.

[0046] Reference Figure 5 , the transition trolley 82 is arranged on the side of the reaction cup holder 23 away from the hopper 21. The transition trolley 82 includes a magnetic seat 821 installed on the moving rail 81 and a cup seat 822 embedded in the magnetic seat 821. A magnet slot 9 is provided along the width direction of the magnetic seat 821, and a cup slot 10 is provided on the cup seat 822. The positions of the cup slot 10 and the magnet slot 9 correspond. A magnet 131 is embedded in the magnet slot 9. A fixing part 824 for fixing the magnet 131 is embedded on the magnetic seat 821. In this application, the fixing part 824 can be fixed by screws, bolts and other fixing parts 824. Since this is a prior art, it will not be repeated here. A control part (not shown in the figure) for controlling the lifting and lowering of the magnet 131 is provided at the bottom of the magnet slot 9.

[0047] During operation, the reaction cup is placed on the cup slot 10 of the transition trolley 82 through the robotic arm assembly 11, and the magnetic beads in the magnetic bead reagent chamber 3 are placed in the reaction cup. The magnet 131 is controlled to rise through the control component so that it can sense the magnetic beads in the reaction cup, thereby adsorbing the substances that do not need to be detected in the reaction cup and retaining the required supernatant.

[0048] The cross section of the transition trolley 82 is convex, the magnetic seat 821 is arranged on both sides of the cup seat 822 and is lower in height than the cup seat 822, the length of the magnetic seat 821 is less than the length of the cup seat 822, and the number of magnet slots 9 is at least one less than the number of cup slots 10. In the schematic diagram of the present application, the number of cup slots 10 is set to 4 and the number of magnet slots 9 is set to 2 as an example, that is, two reaction cups that do not require magnetic attraction and two reaction cups that require magnetic attraction can be placed.

[0049] The transition trolley 82 can be used to place the reaction cup that needs magnetic attraction, and can also be used to place the supernatant after adding the diluent. The test tube cup for placing the supernatant does not need to be magnetically attracted, so it can prevent the position transition of the reagent in the cup slot 10 without the magnet slot 9, and adapt to the positive detection and negative detection in this detection. The height setting of the magnetic attraction seat 821 makes the magnetic attraction point close to the bottom of the reaction cup, so that the supernatant required for detection can be better sucked out.

[0050] Reference Figure 6 and Figure 7 The setting of the mechanical arm assembly 11 can be used to move the reaction cup and install the suction tube to move and add the reagent. The mechanical arm assembly 11 includes a column 111, a slide rail 112 that is perpendicular to the column 111 and slidably connected, a slider 113 that is slidably connected to the slide rail 112, and a gripping arm 114 that is rotatably connected to the slider 113. The gripping arm 114 can grab the reaction cup and move the reaction cup to the drug mixing assembly 42 or the cup holder 822. The slider 113 is connected to a driving member 12, and the driving member 12 can be set as a driving motor 422, a gear rack, etc. This is a prior art and will not be described in detail.

[0051] When the reaction cup needs to be moved, the driving member 12 is started, so that the driving member 12 drives the slider 113 to slide in the slide rail 112, thereby moving the gripping arm 114 to the top of the position where the reaction cup is located, rotating the gripping arm 114, so that the inner wall of the gripping arm 114 abuts against the outer wall of the reaction cup, and then the reaction cup is lifted by rotating the gripping arm 114, and then the driving member 12 is started, and the slider 113 drives the reaction cup to the desired position, and then the reaction cup is put down, and the up and down displacement of the gripping arm 114 needs to be realized by the movement of the slide rail 112 relative to the column 111. This embodiment is only explained by taking the transport reaction cup as an example. When the gripping arm 114 is connected to the suction tube, it can be moved to the magnetic bead reagent compartment 3 to absorb and move the magnetic beads or reagents, and add the magnetic beads or reagents to the reaction cup. Of course, the final reagent cup can also absorb the supernatant by moving the gripping arm 114, which is the same as the aforementioned implementation principle, and will not be repeated here.

[0052] Reference Figure 8 and Fig. 9The medicine mixing component 42 includes a mounting plate 421 disposed on the side of the cup sorter 2 away from the magnetic bead reagent chamber 3, a driving motor 422 disposed in the mounting plate 421, a rotating plate 423 connected to the output shaft of the driving motor 422, and a track plate 424 disposed outside the rotating plate 423. The driving motor 422 can drive the rotating plate 423 to rotate eccentrically on the track plate 424. A placement groove 5 for placing a reaction cup is provided at one end of the rotating plate 423, a mounting groove 6 is provided at the other end of the rotating plate 423, an eccentric rod 425 is fixedly placed in the mounting groove 6, the driving shaft of the driving motor 422 is connected to the eccentric rod 425, a rotating rod 426 is inserted in the center of the track plate 424, and the rotating rod 426 is also penetrated on the rotating plate 423, the rotating plate 423 can do eccentric movement around the rotating rod 426, and the driving motor 422 is disposed at the bottom of the track plate 424.

[0053] When the substance in the reaction cup needs to be shaken, the drive motor 422 is started, and the output shaft of the drive motor 422 drives the rotating disk 423 to make eccentric motion along the range formed by the inner wall of the track disk 424. The setting of the track disk 424 not only limits the rotation range of the rotating disk 423, but also reduces the amplitude of the mixing, avoids the reagent from splashing out of the reaction cup, and avoids the deviation of the test data. The drive motor 422 is installed in the installation disk 421, which further saves the space required for the entire device, thereby further saving the detection cost. Specifically, when the driving motor 422 is started, the output shaft of the driving motor 422 is connected to the eccentric rod 425, so that the output shaft can drive the eccentric rod 425 to rotate, thereby driving the rotating disk 423 to rotate. At this time, a rotating rod 426 inserted in the center of the rotating disk 423 is also provided. The rotating rod 426 further limits the rotation range of the rotating disk 423, so that the rotating disk 423 can perform eccentric movement around the rotating rod 426. When the rotating disk 423 rotates, the rotation trajectory of the rotating disk 423 around the rotating rod 426 is in an 8-shape, making the mixing more uniform.

[0054] A magnetic attraction device 13 is also provided at the bottom of the track plate 424. The magnetic attraction device 13 includes a magnet 131 provided on the placement slot 5 and a lifting assembly (not shown in the figure) provided under the magnet 131. The lifting assembly is used to drive the magnet 131 to rise or fall along the axis direction of the placement slot 5. When the cleaning solution is added to the reaction cup, the lifting assembly drives the magnet 131 to move away from the reaction cup, and the attraction between the magnet 131 and the magnetic beads is continuously reduced. At this time, the oscillation device drives the reaction cup to shake, so that the magnetic beads and the cleaning solution are fully mixed. In this way, the magnetic beads can be fully mixed with the cleaning solution during the magnetic bead cleaning process to reduce the non-target substance residue on the magnetic beads and increase the concentration of the target substance.

[0055] The implementation principle of a reagent transport device and a mass spectrometer in the embodiment of the present application is as follows: after the reaction cup is placed in the hopper 21, under the action of the slope, the reaction cup slides out from the discharge port and falls on the slide 22. Since a cup brim is formed on the top of the reaction cup, the reaction cup can be stuck on the slide 22, and under the action of gravity, it slides to the bottom of the slide 22, and the conveying mechanism transfers the reaction cup to the reaction cup seat 23 through the transfer channel. When the top of the reaction cup slides out of the discharge port first, the lifting mechanism drives the baffle 24 to rise and push the reaction cup back. Under the action of the slope, the reaction cup changes direction and falls smoothly into the slide 22. Then, the reaction cup is placed at the drug transport device 4 through the mechanical arm assembly 11, and the internal standard liquid is added through the liquid supply pump 41. Then, the reaction cup is placed on the transition trolley 82 through the mechanical arm assembly 11, and the magnetic beads are added to the reaction cup. Then, the reaction cup is placed at the drug transport device 4 through the mechanical arm assembly 11, and the drive motor 422 is started. The output shaft of the drive motor 422 is connected to the eccentric rod 425, so that the output shaft can drive the eccentric rod 425 to rotate, thereby driving the rotating disk 423 to rotate. At this time, a rotating rod 426 inserted in the center of the rotating disk 423 is also provided. The rotating rod 426 further limits the rotation range of the rotating disk 423, so that the rotating disk 423 can eccentrically move around the rotating rod 426. After magnetic attraction, the diluent is added and the supernatant is taken after standing, and finally transported to the mass spectrometer 14 for detection through the mechanical arm assembly 11.

[0056] On the other hand, an embodiment of the present application also discloses a reagent transport device and a method for using a mass spectrometer.

[0057] Includes steps: S1, placing the reaction cup: placing the reaction cup in the cup sorter 2, and placing the cup sorter 2 in the cup slot 10 with a magnetic suction slot on the transition trolley 82 through the gripping arm 114; S2, adding internal standard: the reaction cup is moved to the medicine mixing component 42 through the gripping arm 114, and the internal standard liquid is added through the liquid supply pump 41; S3, sample loading: the grab arm 114 goes to the magnetic bead reagent compartment 3 to absorb the sample, goes to the transition trolley 82, and injects the reagent into the reaction cup; S4, adding magnetic beads: the grab arm 114 goes to the magnetic bead reagent compartment 3 to absorb the magnetic bead liquid, goes to the transition trolley 82, and injects the reagent into the reaction cup; S5, vortex oscillation: the gripping arm 114 places the reaction cup in the placement tank 5, and mixes it through the drug mixing component 42; S51 , starting the driving motor 422 , the output shaft of the driving motor 422 drives the eccentric rod 425 to rotate, thereby driving the rotating disk 423 to rotate. At this time, the rotating disk 423 performs eccentric movement around the rotating rod 426 .

[0058] Before magnetic attraction, the liquid in the reaction cup needs to be mixed. The specific operation is: turn on the drive motor 422. The output shaft of the drive motor 422 can drive the eccentric shaft to start driving the rotating disk 423 to move eccentrically around the rotating rod 426, thereby shaking the test liquid and finally achieving uniformity.

[0059] S6, magnetic attraction: the reaction cup is moved again to the cup slot 10 of the transition trolley 82 by the gripping arm 114, and the magnetic beads in the reaction cup are magnetically attracted for 55s-1min5s by the magnet 131 in the magnet slot 9; S61. Place the reaction cup on the cup slot 10 of the transition trolley 82 through the robot arm assembly 11, place the magnetic beads in the magnetic bead reagent chamber 3 into the reaction cup, and control the magnet 131 to rise through the control component. The magnet 131 senses the magnetic beads in the reaction cup and adsorbs the substances in the reaction cup that do not need to be detected.

[0060] During the magnetic attraction process, the reaction cup is first placed on the transition trolley 82 through the robotic arm assembly 11, and the rise of the magnet 131 is controlled by the control component, so that the magnet 131 attracts the magnetic beads in the reaction cup, thereby absorbing the liquid to be tested or the liquid that does not need to be tested in the reaction cup, which is convenient for subsequent detection.

[0061] S7, adding diluent: adding the diluent in the magnetic bead reagent compartment 3 into the reaction cup on the transition trolley 82, and sucking the supernatant with a pipette; S8. Transferring and testing the supernatant: The supernatant extracted from the reaction cup is moved to the sample cup through the gripping arm 114, and the sample cup is sent to the mass spectrometer 14 for testing.

[0062] During operation, the reaction cup is first placed in the cup sorter 2, and the reaction cups are sorted and arranged by the cup sorter 2, and then placed on the transition trolley 82 through the grab arm 114, so as to facilitate the subsequent addition of internal standards and sample loading. Then, the magnetic beads need to be added to the reaction cup through the mechanical arm assembly 11, and then the reaction cup is moved to the reagent mixing assembly 42 for mixing. After mixing, different magnetic suction methods can be used for forward and reverse detection according to different detection requirements. Finally, the detection liquid is obtained by adding a diluent and taking the supernatant, and then sent to the mass spectrometer 14 for detection.

[0063] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A reagent transport device and a mass spectrometer, characterized in that: include: The box (1) forms a space for installing the structure of the reagent transport device; A cup arranger (2), used for arranging the reaction cups; A magnetic bead reagent chamber (3), arranged inside the box (1) and on one side of the cup organizer (2), and used for containing magnetic beads and reagents required to be placed in reaction cups; A medicine transport device (4) is arranged on a side of the cup sorter (2) away from the magnetic bead reagent chamber (3), and the medicine transport device (4) comprises a plurality of liquid supply pumps (41) arranged on one side of the cup sorter (2), a medicine mixing component (42) connected to the liquid supply pumps (41), and a connecting pipe (7) arranged between the liquid supply pumps (41) and the medicine mixing component (42); A transition component (8) is arranged on one side of the magnetic bead reagent chamber (3) and the drug transport device (4), and the transition component (8) comprises a moving rail (81) arranged in the box (1) and a transition trolley (82) running on the moving rail (81); A mechanical arm assembly (11) is arranged on the top of the box (1), and a plurality of the mechanical arm assemblies (11) are provided; A mass spectrometer (14) is arranged on a side of the housing (1) close to the magnetic bead reagent compartment (3) for detecting the reagent after operation.

2. A reagent transport device and mass spectrometer according to claim 1, characterized in that: The transition trolley (82) comprises a magnetic seat (821) mounted on the moving rail (81) and a cup seat (822) embedded in the magnetic seat (821); a magnet groove (9) is provided along the width direction of the magnetic seat (821); a cup groove (10) is provided on the cup seat (822); the positions of the cup groove (10) and the magnet groove (9) correspond to each other; a magnet (131) is embedded in the magnet groove (9); and a fixing part (824) for fixing the magnet (131) is embedded in the magnetic seat (821).

3. A reagent transport device and mass spectrometer according to claim 2, characterized in that: The cross-section of the transition trolley (82) is convex-shaped, the magnetic seat (821) is arranged on both sides of the cup seat (822) and is lower than the cup seat (822), the length of the magnetic seat (821) is less than the length of the cup seat (822), and the number of the magnet slots (9) is at least one less than the number of the cup slots (10).

4. A reagent transport device and mass spectrometer according to claim 3, characterized in that: The mechanical arm assembly (11) comprises a column (111), a slide rail (112) perpendicular to and slidably connected to the column (111), a slider (113) slidably connected to the slide rail (112), and a gripping arm (114) rotatably connected to the slider (113); the gripping arm (114) is capable of gripping a reaction cup and moving the reaction cup to the medicine mixing assembly (42) or the cup holder (822); and the slider (113) is connected to a driving member (12).

5. A reagent transport device and mass spectrometer according to claim 1, characterized in that: The medicine mixing component (42) includes a mounting plate (421) arranged on a side of the cup sorter (2) away from the magnetic bead reagent chamber (3), a driving motor (422) arranged in the mounting plate (421), a rotating plate (423) connected to the output shaft of the driving motor (422), and a track plate (424) arranged outside the rotating plate (423), wherein the driving motor (422) can drive the rotating plate (423) to rotate eccentrically on the track plate (424).

6. A reagent transport device and mass spectrometer according to claim 5, characterized in that: A placement groove (5) for placing a reaction cup is provided at one end of the rotating disk (423), and a mounting groove (6) is provided at the other end of the rotating disk (423). An eccentric rod (425) is fixedly placed in the mounting groove (6). The driving shaft of the driving motor (422) is connected to the eccentric rod (425). A rotating rod (426) is inserted in the center of the track disk (424). The rotating rod (426) is also penetrated on the rotating disk (423). The rotating disk (423) can perform eccentric movement around the rotating rod (426). The driving motor (422) is arranged at the bottom of the track disk (424).

7. A reagent transport device and mass spectrometer according to claim 6, characterized in that: A magnetic attraction device (13) is also provided at the bottom of the track plate (424), and the magnetic attraction device (13) comprises a magnet (131) provided on the placement slot (5) and a lifting assembly provided under the magnet (131), and the lifting assembly is used to drive the magnet (131) to rise or fall along the axial direction of the placement slot (5).

8. A method for using a reagent transport device and a mass spectrometer according to any one of claims 1 to 7, characterized in that: Includes steps: S1, placing the reaction cup: placing the reaction cup in the cup sorter (2), and placing the cup sorter (2) on the transition trolley (82) through the gripping arm (114); S2, adding internal standard: the grab arm (114) moves to the magnetic bead reagent compartment (3) to absorb the internal standard solution, moves to the transition trolley (82), and injects the reagent into the reaction cup; S3, sample loading: the grab arm (114) moves to the magnetic bead reagent compartment (3) to absorb the sample, moves to the transition trolley (82), and injects the reagent into the reaction cup; S4, adding magnetic beads: the grab arm (114) moves to the magnetic bead reagent compartment (3) to absorb the magnetic bead liquid, moves to the transition trolley (82), and injects the reagent into the reaction cup; S5, vortex oscillation: the gripping arm (114) places the reaction cup in the placement tank (5), and mixes it through the medicine mixing component (42); S6, magnetic attraction: the reaction cup is moved again to the cup groove (10) of the transition trolley (82) by the gripping arm (114), and the magnetic beads in the reaction cup are magnetically attracted for 55 seconds to 1 minute 5 seconds by the magnet (131) in the magnet groove (9); S7, adding diluent: adding the diluent in the magnetic bead reagent compartment (3) into the reaction cup on the transition trolley (82), and sucking the supernatant with a pipette; S8, transfer the supernatant and detect: the supernatant extracted from the reaction cup is moved to the sample cup through the gripping arm (114), and the sample cup is sent to the mass spectrometer (14) for detection.

9. The method for using a reagent transport device and a mass spectrometer according to claim 8, characterized in that: Mixing is performed by using a medicine mixing component (42), including the steps of: starting a driving motor (422), wherein the output shaft of the driving motor (422) drives an eccentric rod (425) to rotate, thereby driving a rotating disk (423) to rotate, and at this time, the rotating disk (423) performs an eccentric motion around a rotating rod (426).

10. The method for using a reagent transport device and a mass spectrometer according to claim 8, characterized in that: The magnetic beads in the reaction cup are magnetically attracted for 55 seconds to 1 minute 5 seconds by the magnet (131) in the magnet slot (9), and the steps include: placing the reaction cup on the cup slot (10) of the transition trolley (82) through the mechanical arm assembly (11), placing the magnetic beads in the magnetic bead reagent compartment (3) into the reaction cup, controlling the magnet (131) to rise by the control component, and the magnet (131) and the magnetic beads in the reaction cup are inductively coupled to each other, so that the substances in the reaction cup that do not need to be detected are adsorbed.

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