Compound preparation for fasting induced physiological remodeling of laying hens and application of compound preparation

Abstract

The invention discloses a compound preparation for fasting-induced physiological remodeling of laying hens and application of the compound preparation, and belongs to the technical field of fasting-induced physiological remodeling. By adopting the compound preparation and the method provided by the invention, the intestinal villus height of laying hens and the expression quantity of intestinal barrier related genes and intestinal proliferation related genes can be remarkably improved, the expression quantity of intestinal proinflammatory related genes can be remarkably reduced, the stress injury and inflammation of intestinal tracts can be relieved, and the intestinal health degree of the laying hens can be remarkably improved; the expression quantity of immune globulin A, immune globulin G and immune globulin M can be obviously improved, so that the immune performance of laying hens is improved; with the improvement of intestinal health degree and immunity, fasting-induced physiological remodeling of the laying hens is successfully realized: the weight and laying rate recovery capability of the laying hens after fasting and the yolk color depth in the 30th day after fasting are remarkably superior to those of a control group, and a technical guarantee is provided for delayed raising, cost saving and efficiency improvement of the laying hens.

Description

Technical Field

[0001] The invention relates to the technical field of fasting-induced physiological remodeling, and in particular to a composite preparation for fasting-induced physiological remodeling in laying hens and an application thereof. Background Art

[0002] In recent years, with the rising prices of feed raw materials such as corn and soybean meal, the extension of laying hen breeding has become a global trend. An important task of my country's laying hen industry technology system is to extend the breeding cycle of laying hens, achieve 500 eggs in 700 days, and ensure high egg production rate and egg quality in the late laying period. Fast-induced physiological remodeling (FIPR) is one of the important ways to achieve this goal.

[0003] Fasting-induced physiological remodeling is a method of inducing the remodeling of various functions of laying hens through fasting, among which the shedding and regeneration of feathers (commonly known as forced molting) are prominent features of the process. During the process of fasting-induced physiological remodeling, the physiology and metabolism of laying hens will undergo significant changes. For example, it has a regulatory effect on follicular development. Through fasting, the ovarian function and follicular development of laying hens will change, promoting the development of primary and secondary follicles, thereby extending the laying cycle of laying hens and bringing a second peak of laying for the flock; affecting the synthesis, secretion and uptake of bile acids; effectively alleviating fatty liver caused by metabolic disorders in elderly laying hens; however, during the process of fasting-induced physiological remodeling, it will also induce immunosuppression in laying hens, increase sensitivity to pathogenic bacteria such as Salmonella enteritidis and Escherichia coli, lead to intestinal flora disorders, severe intestinal damage and other problems, and there are fewer corresponding intestinal health care plans. Other studies have found that during the process of inducing physiological remodeling, the intestinal structure is destroyed during fasting, and the content of beneficial intestinal bacteria Bacteroides and its synthetic metabolite vitamin B6 is significantly reduced. Summary of the invention

[0004] The purpose of the present invention is to provide a composite preparation for fasting-induced physiological remodeling in laying hens and its application, so as to solve the problem that in the prior art, during the fasting-induced physiological remodeling process in laying hens, immunosuppression, intestinal flora disorder and severe intestinal damage are easily induced.

[0005] To achieve the above object, the present invention provides a composite preparation for fasting-induced physiological remodeling in laying hens, the composite preparation comprising Bacteroides fragilis ATCC25285+vitamin B6, and the number of viable bacteria of Bacteroides fragilis ATCC25285 in the composite preparation is 1×10 9 CFU / mL, the mass of vitamin B6 is 0.5 mg.

[0006] Preferably, Bacteroides fragilis ATCC25285 is activated using brain heart infusion broth culture medium, and the pH of the culture medium is 7.4±0.2.

[0007] A method for fasting-induced physiological remodeling in laying hens, using the composite preparation for fasting-induced physiological remodeling in laying hens as described above, and the steps are as follows:

[0008] S1. Select healthy laying hens in the late egg-laying period for fasting induction. Cut off water and feed supply on the 1st to 3rd day of fasting. Expose laying hens to light for 8 hours a day and avoid personnel flow and noise pollution near the chicken house.

[0009] S2. Feed supply was interrupted from the 4th to the 15th day of fasting, water was given normally, and a compound preparation for fasting-induced physiological remodeling was administered orally to laying hens. The laying hens were exposed to light for 8 hours per day.

[0010] S3. When the weight loss rate of laying hens is ≥25%, start to resume feeding and gradually extend the lighting time by 30 minutes per day until the 16th hour, and stop extending it after 16 hours.

[0011] Preferably, each laying hen in S2 is gavagely administered 1 mL of the composite preparation for fasting-induced physiological remodeling in laying hens.

[0012] Preferably, in S2, fasting is terminated early when the laying hen mortality rate reaches 4% or above.

[0013] Preferably, the area around the chicken house is sterilized every day during the fasting period.

[0014] Preferably, the method for resuming feeding in S3 is: feeding 30 g / head of basic feed every day on the 1st to 2nd day of resuming feeding; feeding 60 g / head of basic feed every day on the 3rd to 4th day of resuming feeding; feeding 90 g / head of basic feed every day on the 5th day of resuming feeding; feeding 120 g / head of basic feed every day on the 6th to 20th day of resuming feeding; and feeding freely thereafter.

[0015] An application of the composite preparation for fasting-induced physiological remodeling in laying hens as described above in fasting-induced physiological remodeling in laying hens.

[0016] Bacteroides fragilis ATCC25285 is considered a beneficial bacterium and an important component of the intestinal flora. It is beneficial to maintain intestinal homeostasis and plays an important role in promoting the maturation of the immune system, inhibiting intestinal inflammation, and improving the structure of the intestinal flora. It is commonly found in the human intestinal flora and is also present in the oral cavity and upper respiratory tract. Vitamin B6 is a micronutrient necessary for maintaining the healthy growth of animal bodies and has physiological functions such as anti-inflammatory, antioxidant, neuromodulatory and anti-tumor. In the reports on Bacteroides fragilis ATCC25285 and vitamin B6, no research on the combined use of Bacteroides fragilis ATCC25285 and vitamin B6 was found.

[0017] Therefore, the composite preparation for fasting-induced physiological remodeling in laying hens and its application provided by the present invention have the following specific technical effects:

[0018] (1) The composite preparation and method provided by the present invention can successfully achieve fasting-induced physiological remodeling of laying hens: the weight and egg production rate recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical guarantee for extending the breeding of laying hens and saving costs and increasing efficiency;

[0019] (2) The composite preparation and method provided by the present invention can significantly increase the ileal villus height of laying hens, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens.

[0020] (3) The composite preparation and method provided by the present invention can significantly increase the expression levels of immunoglobulins, immunoglobulin G, and immunoglobulin M, thereby improving the immune performance of laying hens. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments of the present invention will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0022] Figure 1 It is the statistical result of egg production rate in different fasting periods in the embodiment of the present invention;

[0023] Figure 2 It is a morphological and histological section diagram (HE5x) of the jejunum of laying hens at different stages in the embodiment of the present invention;

[0024] Figure 3 It is a morphological and histological section diagram (HE5x) of the ileum of laying hens at different stages in the embodiment of the present invention;

[0025] Figure 4It is the expression amount of related genes in the jejunum of laying hens in the embodiment of the present invention, wherein Part A is the relative expression amount of TNF-α at different periods, Part B is the relative expression amount of IL-15 at different periods, Part C is the relative expression amount of IL-6 at different periods, Part D is the relative expression amount of IL-18 at different periods, Part E is the relative expression amount of Claudin-1 at different periods, Part F is the relative expression amount of occludin-1 at different periods, Part G is the relative expression amount of Lgr5 at different periods, and Part H is the relative expression amount of CDK1 at different periods;

[0026] Figure 5 It is the expression level of ileum-related genes in laying hens in the embodiment of the present invention, wherein Part A is the relative expression level of TNF-α at different periods, Part B is the relative expression level of IL-15 at different periods, Part C is the relative expression level of IL-6 at different periods, Part D is the relative expression level of IL-18 at different periods, Part E is the relative expression level of Claudin-1 at different periods, Part F is the relative expression level of occludin-1 at different periods, Part G is the relative expression level of Lgr5 at different periods, and Part H is the relative expression level of CDK1 at different periods. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0028] In order to make the purpose, technical scheme and advantages of the present application clearer, more thorough and more complete, the technical scheme of the present invention is clearly and completely described below through the accompanying drawings and examples. The following detailed descriptions are all descriptions of the embodiments, and are intended to provide further detailed descriptions of the present invention. Unless otherwise specified, all technical terms used in the present invention have the same meaning as those generally understood by those skilled in the art to which the present application belongs.

[0029] The instruments, equipment, reagents and materials used in the examples were obtained through commercial channels, and the experimental methods in the examples are conventional methods in the art unless otherwise specified.

[0030] Embodiment 1

[0031] Fasting-induced physiological remodeling was performed on laying hens. The specific settings are shown in Table 1. The specific steps are as follows:

[0032] Table 1

[0033]

[0034] S11. A total of 288 healthy, disease-free 420-day-old bantam chickens weighing 1300-1500 g were selected and randomly divided into 4 groups, with 9 replicates in each group, 8 chickens in each replicate, and 72 chickens in each group. One group was the control group and three groups were the treatment groups: Bacteroides fragilis ATCC25285 group, vitamin B6 group, and Bacteroides fragilis ATCC25285 + vitamin B6 combination preparation group.

[0035] S12, the experimental chicken house is strictly disinfected by fumigation with formaldehyde and potassium permanganate, and then the laying hens selected in step S11 are transferred to the chicken house and conventionally fed (adaptive feeding) with a basic diet for 10 days. The composition and nutritional level of the basic diet are shown in Table 2.

[0036] Table 2

[0037] project ％ Nutritional Levels ％ corn 62.50 Metabolizable Energy 11.20 Soybean meal 25.50 Crude protein 15.80 Stone powder 9.20 calcium 3.78 Oil powder 0.80 Available Phosphorus 0.28 Premix 2.00 Lysine 0.71 total 100.00 Methionine 0.66

[0038] The premix provides VA12000IU, VD33000IU, VE30IU, VK10mg, VB112mg, VB120.05mg, biotin16.4mg, niacin30mg, pantothenic acid10mg, folic acid1mg, choline500mg, Cu45mg, Fe80mg, Zn85mg, Mn70mg, I0.5mg, Se0.0mg per kg of feed. Crude protein and calcium are measured values, and the other nutrient levels are calculated values.

[0039] S13. Then add 1% shell powder to the basic feed and add multivitamin (Yipan) to the drinking water for ten days. Adding 1% shell powder can reduce the egg breaking rate after the beginning of molting and the overdraft of blood calcium in the chicken after molting, and adding multivitamin to the drinking water can prevent stress response.

[0040] S14. Number the chickens in each treatment group, randomly select 10 chickens from each treatment group, and monitor the weight changes of the numbered chickens in the later stage to estimate the weight changes of the entire chicken group. The day before the fasting induction (F0) was started, the weight and number of eggs laid by the laying hens in the control group and the experimental group were weighed and recorded, and the laying rate (total number of eggs laid / total number of laying hens) was calculated, and the feed trough was cleaned and the water in the water line was drained.

[0041] The weight of F0 laying hens was the average weight of 40 laying hens randomly selected from the four treatment groups, and the egg production rate of F0 was the average egg production rate of 288 laying hens in the experiment. The average weight of F0 was 1248 g and the egg production rate was 37.35%.

[0042] S15, fasting induction period (F) settings are shown in Table 1:

[0043] (1) On the first 3 days after fasting induction, water and feed (basic diet) were cut off, and light was on for 8 h. During this period, the flow of people in the chicken house was reduced as much as possible, the environment was kept quiet, and the influence of other external factors other than water and feed cutoff was avoided.

[0044] (2) From the 4th to the 15th day after the fasting induction, the feed and water were cut off. The control group was gavaged with normal saline, and the Bacteroides fragilis ATCC25285 group was gavaged with a live bacterial count of 1×10 9 The live bacterial preparation of Bacteroides fragilis ATCC25285 was administered daily with 0.5 mg of vitamin B6 in the vitamin B6 group and 1×10 live bacterial count of Bacteroides fragilis ATCC25285 in the combined preparation group. 9 CFU live bacteria preparation + 0.5mg vitamin B6, light exposure for 8h.

[0045] S16. After the fasting induction began, the weights of the laying hens in the control group and the treatment group were weighed and recorded at 16:00 every 3 days. The weight loss rate was calculated using the following formula (the results are shown in Table 4). The egg production rate was counted and recorded (the results are shown in Table Figure 1 ). When the average weight loss rate of the laying hen group reaches 25%, feed intake will be resumed. The weight loss rate is calculated using the following formula:

[0046] Weight loss rate of Fx (%) = (body weight at F0 - body weight at Fx) / body weight at F0 × 100%, where F is the fasting period, F0 is the day before the start of fasting, and Fx is the xth day of fasting.

[0047] Based on the monitoring of the chicken flock weight, the fasting period was determined to be 15 days.

[0048] S17, recovery feeding period (R), the 16th day from the fasting induction period (recorded as the first day of the recovery feeding period, R1) enters the recovery feeding period, during which the chickens drink water normally, and the feeding conditions during the recovery feeding period are as follows:

[0049] Feed 30g of basic feed per head per day on the first 1-2 days after resuming feeding;

[0050] Feed 60g of basic feed per head per day on the 3rd to 4th day after resuming feeding;

[0051] On the fifth day of resuming feeding, feed 90 g of basic feed per head per day;

[0052] Feed 120g of basic feed per head per day from the 6th to the 20th day after the resumption of feeding;

[0053] Thereafter, food was available ad libitum.

[0054] During the gradual recovery of feeding, the light intensity was increased by 30 min per day until it reached 16 h / day.

[0055] Embodiment 2

[0056] The production performance and egg quality of laying hens were investigated one day before fasting, during the fasting induction period, and during the recovery period. The specific steps are as follows:

[0057] S21. Record the number of eggs laid by each group of laying hens at 16:00 every day, and then take the average of the daily egg production rates in the five stages of F0-F3, F4-F5, R17-R22, R23-R26, and R27-R30 as the egg production rate of this stage; record the body weight of each group every three days and calculate the weight loss rate.

[0058] Rx weight loss rate (%) = (weight at F0 - Rx weight) / F0 weight × 100%, where

[0059] R is the recovery period, F0 is the day before the start of fasting, and Rx represents the xth day of resuming feeding.

[0060] S22. During the R30 period, i.e., the 30th day after the end of fasting, 25 eggs were randomly collected from each group for determination of egg quality indicators using a multifunctional egg quality analyzer. The measured indicators included egg weight, egg shape index, eggshell thickness, eggshell strength, eggshell weight, egg white height, and yolk color.

[0061] Embodiment 3

[0062] The immune function, intestinal tissue morphology, and intestinal related gene expression levels of laying hens were investigated one day before fasting, during the fasting induction period, and during the recovery period. The specific steps are as follows:

[0063] S31. Select one laying hen with close to average weight from each replicate in F0, F15, R5, R30, treatment group and control group, with a total of 9 replicates. Collect blood from subwing vein with a coagulant tube, centrifuge at 1265×g for 10 min, collect serum, quickly freeze in liquid nitrogen and transfer to -80℃ for storage for detection of serum immune indices.

[0064] The levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were detected using an ELASA kit.

[0065] S32. After blood collection, the experimental chickens were slaughtered, and 1 cm of the jejunum and ileum were collected and slowly rinsed with 0.9% saline, and then stored in a 10 mL centrifuge tube containing 4% paraformaldehyde (fixative) for fixation. After the collected jejunum and ileum segments were fixed for 24 hours, the intestinal segments were taken out from the fixative and embedded in paraffin, made into slices and stained with hematoxylin-eosin (HE), and observed and photographed under an optical microscope. The results are as follows Figure 2 , 3The villus height and crypt depth of each intestinal segment were measured using MoticDS Assistant Lite software, and the villus-crypt ratio (villus height / crypt depth) was calculated. The results are shown in Table 6.

[0066] S33. Collect jejunal and ileal tissue samples, place them in 1.5 mL sterile enzyme-free centrifuge tubes, quickly freeze them in liquid nitrogen, and finally store them in a -80°C refrigerator for subsequent measurements.

[0067] The total RNA of jejunum and ileum at each sampling point was extracted using a kit, and the target gene was detected by real-time fluorescence quantitative PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference gene. -ΔΔCt The relative mRNA expression of the target gene in the intestinal segment was calculated by the method. The results are as follows Figure 4 and Figure 5 The primer sequences and fragment size information of the reference gene and target gene are shown in Table 3.

[0068] Table 3

[0069]

[0070]

[0071] Results Analysis

[0072] The experimental data were preliminarily collated and analyzed using Excel 2010, and then SPSS 26.0 software was used for data analysis. Production performance and intestinal tissue morphology were analyzed by one-way analysis of variance and Duncan's method for multiple comparisons; intestinal gene expression and serum immune indexes were analyzed by t-test, and the results were expressed as "mean ± standard deviation", and P < 0.05 indicated significant differences. GraphPad Prism 8.0 software was used for data and graphics processing.

[0073] In the table, data in the same row with different lowercase letters in the shoulders indicate significant differences (P<0.05). The control group is represented by NC, Bacteroides fragilis ATCC25285 is represented by B.fragilis, vitamin B6 is represented by VB6, and the combined preparation group of Bacteroides fragilis ATCC25285 and vitamin B6 is represented by B.fragilis+VB6.

[0074] (1) The statistical results of weight loss rate (%) of laying hens in the experimental group and the control group are shown in Table 4.

[0075] On the 45th day, i.e., the R30 period, the weight recovery of the Bacteroides fragilis ATCC25285+vitamin B6 combination preparation group was better than that of the other groups, but there was no significant difference among the groups at each stage (P>0.05).

[0076] Table 4

[0077]

[0078]

[0079] (2) Effects of Bacteroides fragilis ATCC25285 and vitamin B6 on egg production in laying hens during fasting-induced physiological remodeling Figure 1 As shown, in F1-F3, the average egg production rate of the NC group dropped to 25.51%, the average egg production rate of the Bacteroides fragilis ATCC25285 group dropped to 28.70%, the average egg production rate of the vitamin B6 group dropped to 31.48%, and the average egg production rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group dropped to 27.89%. In F4-F5, the average egg production rate of the NC group dropped to 2.55%, the average egg production rate of the Bacteroides fragilis ATCC25285 group dropped to 2.09%, the average egg production rate of the vitamin B6 group dropped to 4.23%, and the average egg production rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group dropped to 1.41%. F6-R17 flocks began to enter the off-laying period. The NC group, Bacteroides fragilis ATCC25285 group, vitamin B6 group and Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group began to lay eggs again at R20, R18, R21 and R19, respectively, and the egg laying rate of each group gradually increased. In R17-R22, the average egg laying rate of the NC group was 7.08%, the average egg laying rate of the Bacteroides fragilis ATCC25285 group was 10.37%, the average egg laying rate of the vitamin B6 group was 7.96%, and the average egg laying rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was 10.71%; in R23-R26, the NC The average egg laying rate of the NC group was 15.27%, the average egg laying rate of the Bacteroides fragilis ATCC25285 group was 20.21%, the average egg laying rate of the vitamin B6 group was 15.91%, and the average egg laying rate of the weak Bacteroides ATCC25285 + vitamin B6 compound preparation group was 20.07%; in R27-R30, the average egg laying rate of the NC group was 31.83%, the average egg laying rate of the Bacteroides fragilis ATCC25285 group was 39.54%, the average egg laying rate of the vitamin B6 group was 31.17%, and the average egg laying rate of the weak Bacteroides ATCC25285 + vitamin B6 compound preparation group was 42.64%.

[0080] (3) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on egg quality during fasting-induced physiological remodeling are shown in Table 5. Compared with R30NC, the yolk color of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly darker (P<0.05); the eggshell weight and eggshell strength of the vitamin B6 group were significantly improved (P<0.05), and there were no significant differences in egg weight, egg shape index, and albumen height among the groups (P>0.05).

[0081] Table 5

[0082]

[0083] (4) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the intestinal morphology of laying hens during fasting-induced physiological remodeling are shown in Table 6. Compared with the control group, the crypt depth of the jejunum in the vitamin B6 group was significantly thickened at F15 (P<0.05); the villus height of the ileum in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly increased (P<0.05). The villus height, crypt depth, and villus-crypt ratio of the intestine at other stages were not significant (P>0.05).

[0084] Table 6

[0085]

[0086]

[0087] Note: The unit of villus height is μm, and the unit of crypt depth is μm

[0088] (5) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the immune indicators of laying hens during fasting-induced physiological remodeling were investigated, and the results are shown in Table 7. IgA in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the R5 period (P<0.05); IgG in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the F15, R5, and R30 periods (P<0.05); IgM in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the R5 period (P<0.05).

[0089] Table 7

[0090]

[0091]

[0092] (6) Effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the expression of genes related to the jejunum and ileum of laying hens during fasting-induced physiological remodeling Figure 4 , 5 Compared with the control group, the expression levels of intestinal barrier-related genes occludin-1 and Claudin-1 and intestinal proliferation-related genes CDK1 and Lgr5 in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group were significantly increased (P<0.05), and the expression levels of intestinal pro-inflammatory genes TNF-α, IL-6 and IL-18 were significantly decreased (P<0.05).

[0093] The results showed that during the fasting-induced physiological remodeling process of laying hens, the compound preparation of Bacteroides fragilis ATCC25285+vitamin B6 was superior to the treatment of adding Bacteroides fragilis ATCC25285 and vitamin B6 alone. The compound preparation of Bacteroides fragilis ATCC25285+vitamin B6 could improve the production performance of laying hens during the fasting-induced physiological remodeling process, improve their intestinal tissue morphology and structure, improve intestinal barrier function and proliferation-related genes, reduce the expression of intestinal inflammation-related genes, enhance the body's immune function, and thus maintain the intestinal health of laying hens.

[0094] Therefore, the composite preparation and method provided by the present invention can significantly increase the ileal villus height of laying hens, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes, significantly reduce the expression levels of intestinal pro-inflammatory related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens; the expression levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M can be significantly increased, thereby improving the immune performance of laying hens; with the improvement of intestinal health and immunity, fasting-induced physiological remodeling of laying hens is successfully achieved: the weight and egg production rate recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical guarantee for extending the breeding of laying hens, saving costs and increasing efficiency.

[0095] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A composite preparation for fasting-induced physiological remodeling in laying hens, characterized in that: The compound preparation includes Bacteroides fragilis ATCC25285+vitamin B6. The number of viable bacteria of Bacteroides fragilis ATCC25285 in the compound preparation is 1×10 9 CFU / mL, the mass of vitamin B6 is 0.5 mg.

2. A composite preparation for fasting-induced physiological remodeling in laying hens according to claim 1, characterized in that: Bacteroides fragilis ATCC25285 was activated using brain heart infusion broth medium, and the pH of the medium was 7.4±0.

2.

3. A method for fasting-induced physiological remodeling in laying hens, using the composite preparation for fasting-induced physiological remodeling in laying hens as claimed in claim 1, characterized in that: Here are the steps: S1. Select healthy laying hens in the late egg-laying period for fasting induction. Cut off water and feed supply on the 1st to 3rd day of fasting. Expose the laying hens to light for 8 hours a day and avoid the flow of people and noise pollution near the chicken house. S2. Feed supply was interrupted from the 4th to the 15th day of fasting, water was given normally, and a compound preparation for fasting-induced physiological remodeling was administered orally to laying hens. The laying hens were exposed to light for 8 hours per day. S3. When the weight loss rate of laying hens is ≥25%, start to resume feeding and gradually extend the lighting time by 30 minutes per day until the 16th hour, and stop extending it after 16 hours.

4. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: In S2, each laying hen was gavageed with 1 mL of the compound preparation for fasting-induced physiological remodeling in laying hens.

5. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: In S2, fasting was terminated early when the mortality rate of laying hens reached 4% or above.

6. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: During the fasting period, disinfect the area around the chicken house every day.

7. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: The feeding recovery method in S3 is: feed 30g / head basic feed every day on the 1st and 2nd days of feeding recovery; feed 60g / head basic feed every day on the 3rd and 4th days of feeding recovery; feed 90g / head basic feed every day on the 5th day of feeding recovery; feed 120g / head basic feed every day on the 6th to 20th days of feeding recovery; and feed freely after that.

8. Use of the composite preparation for fasting-induced physiological remodeling in laying hens as claimed in claim 1 in fasting-induced physiological remodeling in laying hens.

Images