Intelligent wound dressing constructed based on biodegradable material system and preparation method thereof

Abstract

The invention discloses an intelligent wound dressing constructed based on a biodegradable material system and a preparation method of the intelligent wound dressing, and belongs to the technical field of biological materials. The preparation method of the intelligent wound dressing comprises the following steps: dissolving PCL particles and collagen in hexafluoroisopropanol, stirring overnight, adding anthocyanin powder, and stirring to obtain an electrostatic spinning solution; the electrostatic spinning solution is sucked into a 10 mL injector, the injector is placed on a micro-injection pump, electrostatic spinning is carried out, a receiving device is a rotatable roller, a spinning film of a multi-stage porous structure is obtained, vacuum drying is carried out, and the wound dressing of the multi-stage porous structure is obtained; and data are uploaded through the signal acquisition system. The intelligent wound dressing constructed on the basis of the biodegradable material system has the functions of exporting wound exudate, dynamically monitoring pH and treating, the dressing can be autonomously absorbed and degraded in the wound healing process, secondary damage caused by tearing due to dressing replacement is avoided, and therefore the wound healing efficiency is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomaterials, and specifically relates to an intelligent wound dressing constructed based on a biodegradable material system and a preparation method thereof. Background Art

[0002] With the aging of the global population and the increase in chronic diseases, the prevalence of chronic wounds such as pressure sores, diabetic foot ulcers, and venous ulcers of the lower extremities remains high, placing a heavy economic burden on the healthcare system. Traditional wound dressings such as gauze, plasters, and bandages mainly provide physical protection, but have poor moisturizing and bactericidal effects, and may cause tissue adhesion and worsening infection when replaced. Modern dressings such as films, foams, and gels can better absorb exudate, maintain a moist environment, and can load antimicrobial therapeutic factors to promote cell growth and accelerate wound healing. However, these dressings lack dynamic perception of the wound microenvironment and healing status, and rely on the experience and judgment of medical staff, which may lead to misdiagnosis and unnecessary treatment.

[0003] In order to accurately assess the wound status, it is usually necessary to collect wound exudate for biochemical pathological testing, which is a time-consuming, labor-intensive and medical resource-consuming process. With the rapid development of flexible electronic technology, smart dressings have emerged. By organically combining wearable flexible sensors with drug release, they can sense changes in physiological parameters during wound healing and provide precise treatment based on the dynamic needs of the wound. Researchers have developed multi-channel and multi-modal sensor arrays to achieve wireless and continuous monitoring of the wound environment, and combined thermal regulation therapy, exogenous electrical stimulation, and stimuli-responsive controlled drug delivery to perform combined treatments for anti-inflammatory, antibacterial and tissue regeneration. These smart dressing systems can effectively improve the efficiency of wound healing and have been verified in animal models.

[0004] Although smart dressings have made great progress, most of the smart dressings currently developed rely on complex and expensive CMOS processes, non-degradable inert metal electrodes, and polymer substrate films with insufficient air permeability. The replacement and removal of dressings cannot be avoided, and there is a risk of secondary injury to the wound surface. Therefore, the research and development of smart dressings still faces challenges and requires further innovation and improvement to meet clinical needs. Summary of the invention

[0005] The present invention aims to provide an intelligent wound dressing constructed based on a biodegradable material system and a preparation method thereof. The dressing has the functions of wound exudate extraction, pH dynamic monitoring and treatment. The dressing can achieve autonomous absorption and degradation during the wound healing process, avoiding secondary tearing damage caused by dressing replacement, thereby improving the wound healing efficiency.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is as follows: A method for preparing an intelligent wound dressing based on a biodegradable material system comprises the following steps: S1. Prepare electrospinning solution: dissolve PCL particles and collagen in hexafluoroisopropanol, stir overnight, then add anthocyanin powder and stir to obtain electrospinning solution; S2. Electrospinning to prepare a multi-level porous structure dressing: The electrospinning solution obtained in step S1 is sucked into a 10 mL syringe, which is placed on a micro-injection pump for electrospinning. The receiving device is a rotatable drum to obtain a multi-level porous structure spinning membrane, which is vacuum dried to obtain a multi-level porous structure wound dressing; S3. Upload data through the signal acquisition system.

[0007] Preferably, in step S1, the mass ratio of PCL particles, collagen and anthocyanin is 10:2:1.

[0008] Preferably, in step S1, PCL particles and collagen are dissolved in hexafluoroisopropanol, stirred at 600 rpm overnight, anthocyanin powder is added, and stirred at 600 rpm for 3 hours to obtain an electrospinning solution.

[0009] Preferably, in step S2, during the electrospinning process, the process parameters are as follows: voltage of 15 kV, flow rate of 2 mL / h-12 mL / h, receiving speed of 100 rpm, and receiving time of 20-120 min.

[0010] Preferably, in step S3, the signal acquisition system is composed of a micro single chip control module, a signal processing module and five groups of red light LEDs and photodiode coupling units.

[0011] Preferably, the micro single-chip control module includes multiple AD conversion channels.

[0012] Preferably, the AD conversion channels are respectively connected to five groups of red light LEDs and photodiode coupling units.

[0013] Preferably, the photodiode monitors the intensity of the red LED light reflected by the dressing in real time, converts the optical signal into an electrical signal, and performs real-time detection of the pH changes at the center and surrounding of the wound.

[0014] Preferably, the signal acquisition system processes the photoelectric signal of the AD conversion channel, and uploads the processed signal to the data terminal through wireless transmission technology or wired transmission technology.

[0015] The present invention also provides an intelligent wound dressing based on a biodegradable material system prepared by the preparation method.

[0016] Compared with the prior art, the present invention has the following advantages and technical effects: The present invention provides an intelligent wound dressing by innovatively adopting a multi-level porous interconnected structure design and combining it with a biodegradable material system. The dressing can be gradually degraded and absorbed during the wound healing process, effectively avoiding secondary damage caused by dressing replacement, and significantly improving the wound healing efficiency. The multi-level porous interconnected structure constructed by the electrospinning process uses capillary action to efficiently guide the wound exudate, effectively reducing the risk of wound infection. This structural design not only speeds up the extraction of exudate, but also reduces the growth of bacteria around the wound, providing a cleaner healing environment for the wound. The PCL, collagen and anthocyanin selected by the present invention as core materials not only promote cell proliferation, but also respond significantly to pH values, realizing dynamic monitoring and precise treatment of wounds. Compared with traditional intelligent dressings that rely on complex CMOS processes and non-degradable metal electrodes, the biodegradable material system of the present invention reduces production costs, while avoiding expensive replacement and removal processes, and has better economy and practicality. And due to the gradual degradation characteristics of the dressing, patients do not need to change dressings frequently, reducing the inconvenience and pain of patients and improving patient compliance.

[0017] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a simulation effect diagram of the exudate extraction effect of the PCL / Coll / A spun film dressing provided in Example 1; Figure 2 This is an SEM image of the spinning structure of the PCL / Coll / A spinning film dressing provided in Example 1, with a scale of 10 μm; Figure 3 A line graph showing the fiber diameter distribution of the spun films provided in Examples 1-4; Figure 4 The response difference results of the dressing provided in Example 1 under different pH conditions; Figure 5 This is a statistical graph of the cell proliferation experiment of the dressings provided in Examples 1-3. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is further described below through the accompanying drawings and embodiments.

[0020] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0021] In the present invention, unless otherwise specified, other test materials and instruments and equipment are conventional test materials in the art and can be purchased through commercial channels.

[0022] Example 1 This example provides a method for preparing an intelligent wound dressing based on a biodegradable material system, comprising the following steps: S1. Preparation of electrospinning solution: 0.6 g PCL particles and 0.12 g collagen were dissolved in 4 mL hexafluoroisopropanol, stirred overnight at 600 rpm in a magnetic stirrer, and then 0.06 g anthocyanin powder was added, and stirred at 600 rpm in a magnetic stirrer for 3 h to obtain an electrospinning solution; S2. Preparation of multi-level porous structure dressing by electrospinning: The electrospinning solution obtained in step S1 was sucked into a 10 mL syringe, placed on a micro-injection pump, and electrospinning was performed at a voltage of 15 kV, a flow rate of 8 mL / h, a receiving speed of 100 rpm, and a receiving time of 30 min. The receiving device was a rotatable drum to obtain a multi-level porous structure spinning membrane, which was vacuum dried to obtain a PCL / Coll / A spinning film dressing; S3. Upload data through a signal acquisition system consisting of a micro single-chip microcomputer control module, a signal processing module, and five groups of red light LEDs and photodiode coupling units. The micro single-chip microcomputer control module includes an AD conversion channel, which is respectively connected to the five groups of red light LEDs and photodiode coupling units to monitor the intensity of the red light LED reflected by the dressing in real time, convert the optical signal into an electrical signal, and perform real-time detection of the pH changes at the center of the wound and around it. The signal acquisition system processes the photoelectric signals of the five channels and uses Bluetooth wireless transmission technology to upload the processed signals to the data terminal.

[0023] Example 2 The preparation method is the same as that of Example 1, except that during the electrospinning process, the flow rate is 2 mL / h and the receiving time is 120 min.

[0024] Example 3 The preparation method is the same as that of Example 1, except that during the electrospinning process, the flow rate is 4 mL / h and the receiving time is 60 min.

[0025] Example 4 The preparation method is the same as that of Example 1, except that during the electrospinning process, the flow rate is 8 mL / h and the receiving time is 30 min.

[0026] Comparative Example 1 S1. Preparation of electrospinning solution: 0.6 g PCL particles were dissolved in 4 mL hexafluoroisopropanol and stirred overnight at 600 rpm using a magnetic stirrer to obtain an electrospinning solution; S2. Preparation of dressing by electrospinning: The electrospinning solution obtained in step S1 was sucked into a 10 mL syringe, placed on a micro-injection pump, and electrospinning was performed at a voltage of 15 kV, a flow rate of 8 mL / h, a receiving speed of 100 rpm, and a receiving time of 30 min. The receiving device was a rotatable drum. Vacuum drying was performed to obtain a PCL spun film dressing.

[0027] Comparative Example 2 S1. Preparation of electrospinning solution: 0.6 g PCL particles and 0.12 g collagen were dissolved in 4 mL hexafluoroisopropanol and stirred overnight at 600 rpm with a magnetic stirrer to obtain an electrospinning solution; S2. Preparation of dressing by electrospinning: The electrospinning solution obtained in step S1 was sucked into a 10 mL syringe, placed on a micro-injection pump, and electrospinning was performed at a voltage of 15 kV, a flow rate of 8 mL / h, a receiving speed of 100 rpm, and a receiving time of 30 min. The receiving device was a rotatable drum. Vacuum drying was performed to obtain PCL / Coll spun film dressing.

[0028] The effects of the spun film dressing provided in the above examples were verified by the following experiments.

[0029] 1. The exudate extraction effect of the PCL / Coll / A spun film dressing provided in Example 1 was simulated, and the results were as follows: Figure 1 .

[0030] Depend on Figure 1 It can be seen that the multilayer composite structure with gradually decreasing porosity from bottom to top has a better water absorption effect.

[0031] 2. The PCL / Coll / A spun film dressing provided in Example 1 was subjected to electron microscopy test. The results are as follows: Figure 2 .

[0032] Depend on Figure 2 It can be seen that as the spinning flow rate increases, the fiber diameter gradually increases.

[0033] 3. The electron microscope images of the different spinning films provided in Examples 1-4 were fitted to obtain the fiber diameter distribution of the films at different spinning flow rates.

[0034] Depend on Figure 3 It can be seen that when the spinning flow rates are 2, 4, 8, and 12 mL / h, the fiber diameters are 0.587, 1.002, 1.509, and 1.806 µm, respectively, showing a good linear relationship.

[0035] 4. Use a test solution with a pH of 1 to 13 to test the pH response characteristics of the dressing film provided in Example 1. Use a disposable plastic pipette to absorb 1 mL of the test solution and drop it on the surface of the spun film with an area of ​​2 cm×2 cm. After the solution is absorbed and stabilized, take photos and record the results.

[0036] Depend on Figure 4 It can be seen that the dressing film has significant color response differences at different pH values.

[0037] 5. The dressings provided in Example 1 and Comparative Examples 1-2 were subjected to a cell proliferation test. The test protocol was as follows: the PCL, PCL / Coll, and PCL / Coll / A electrospun films were immersed in ethanol for about 1 min for disinfection, and then rinsed with phosphate buffer at least 3 times. The films were air-dried on a clean bench, and about 5×10 4 cells were inoculated on each sample. Subsequently, 250 μL of the solution was added to each sample and incubated for 45 min. After incubation, 100 μL of the supernatant of the reaction solution was extracted from the 96-well plate and the absorbance was measured at a wavelength of 440 nm using an ELISA reader. The results are shown in Figure 5 .

[0038] Depend on Figure 5 It can be seen that the PCL / Coll / A film exhibits the highest absorbance, indicating that the film has a good effect in promoting cell proliferation.

[0039] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A method for preparing an intelligent wound dressing based on a biodegradable material system, characterized in that: The steps include: S1. Prepare electrospinning solution: dissolve PCL particles and collagen in hexafluoroisopropanol, stir overnight, then add anthocyanin powder and stir to obtain electrospinning solution; S2. Electrospinning to prepare a multi-level porous structure dressing: The electrospinning solution obtained in step S1 is sucked into a 10 mL syringe, which is placed on a micro-injection pump for electrospinning. The receiving device is a rotatable drum to obtain a multi-level porous structure spinning membrane, which is vacuum dried to obtain a multi-level porous structure wound dressing; S3. Upload data through the signal acquisition system.

2. The preparation method according to claim 1, characterized in that: In step S1, the mass ratio of PCL particles, collagen and anthocyanin is 10:2:

1.

3. The preparation method according to claim 1, characterized in that: In step S1, PCL particles and collagen were dissolved in hexafluoroisopropanol and stirred at 600 rpm overnight, and then anthocyanin powder was added and stirred at 600 rpm for 3 h to obtain an electrospinning solution.

4. The preparation method according to claim 1, characterized in that: In step S2, during the electrospinning process, the process parameters are as follows: voltage of 15 kV, flow rate of 2 mL / h-12 mL / h, receiving speed of 100 rpm, and receiving time of 20-120 min.

5. The preparation method according to claim 1, characterized in that: In step S3, the signal acquisition system is composed of a micro single-chip control module, a signal processing module, and five groups of red light LEDs and photodiode coupling units.

6. The preparation method according to claim 1 or 5, characterized in that: The micro single chip control module comprises a plurality of AD conversion channels.

7. The preparation method according to claim 1 or 6, characterized in that: The AD conversion channels are respectively connected to five groups of red light LEDs and photodiode coupling units.

8. An intelligent wound dressing based on a biodegradable material system prepared by the preparation method according to any one of claims 1 to 7.

Images