TPPA reaction device

Abstract

A TPPA reaction device comprises a base, a reaction plate, a supporting rod and a liquid adding seat, the reaction plate is detachably placed on the base, a plurality of reaction unit pipes which are evenly distributed in transverse and vertical columns are arranged on the reaction plate, the reaction unit pipes are used for carrying out TPPA reaction and are slidably installed on the base, the supporting rod is right opposite to the reaction unit pipes in different vertical columns through sliding of the liquid adding seat, and the liquid adding seat is arranged on the supporting rod. A positioning groove is formed in the supporting rod, during liquid dropping, a dropper is placed in the positioning groove, and the dropper is supported through the positioning groove, so that a sample diluent is dropped into the reaction unit tube by the dropper, and the sample diluent is prevented from being in contact with an experimenter or being dropped out of the reaction unit tube; the supporting rod is connected to the liquid adding base in a swinging mode and / or a movable mode through the swinging groove, the supporting rod slides in the swinging groove, so that the positioning groove is right opposite to different reaction unit tubes in the same vertical column, and liquid dropping and sample adding can be conveniently conducted on the different reaction unit tubes through the dropper.

Description

Technical Field

[0001] The present invention belongs to the technical field of detection devices, and in particular relates to a TPPA reaction device. Background Art

[0002] TPPA is one of the serological tests for Treponema pallidum-specific antibodies. Using the principle of antigen-antibody agglutination reaction, purified Treponema pallidum bacterial components are coated on gelatin particles to prepare sensitized particles, which bind to Treponema pallidum-specific antibodies in human serum or plasma, producing a visible agglutination reaction. Currently, reaction plates with multiple reaction unit tubes are often used for experiments on the market. However, the reaction plates on the market have the following main shortcomings: (1) The TPPA sample diluent is a colorless liquid. When the reaction plate is placed in a biosafety cabinet for operation, the added sample diluent cannot be visually observed, resulting in repeated addition of samples; (2) The sample is added to the reaction plate by the experimenter holding a dropper, which is prone to shaking, causing the sample to drip out of the reaction unit tube. Summary of the Invention

[0003] In view of the above shortcomings, the technical problem to be solved by the present invention is to provide a TPPA reaction device, which facilitates the experimenter to add colorless sample diluent into the reaction unit tube of the reaction plate, prevents the phenomenon of missing addition of the reaction unit tube, and avoids repeated addition or operational errors.

[0004] In order to solve the above technical problems, the technical solution adopted by the present invention is a TPPA reaction device, comprising: a base; A reaction plate is provided with a plurality of reaction unit tubes evenly arranged in horizontal and vertical rows, and the reaction plate can be detachably placed on the base; A support rod, used to support the dropper; The liquid adding seat is slidably mounted on the base, and the support rod is slidably mounted on the liquid adding seat. The support rod is directed to face the reaction unit tubes in different vertical rows by sliding the liquid adding seat. A swing groove is provided in the liquid adding seat. The support rod is swingably and / or movably connected to the swing groove. A directional sliding mechanism for controlling the unidirectional movement of the support rod and a sliding limiter for limiting the support rod after movement are provided in the swing groove. The support rod is separated from the sliding limiter by swinging the support rod in the swing groove, and is moved to above different reaction unit tubes by sliding the support rod in the swing groove.

[0005] As a preferred solution of the present invention, the directional sliding mechanism includes a first roller and a movable directional assembly that are relatively arranged and abut against the support rod. The first roller is rotatably installed in the swing groove, and the movable directional assembly is slidably installed in the swing groove.

[0006] As a preferred solution of the present invention, the movable orienting assembly includes a movable bracket and a second roller with a one-way rotation function. The movable bracket is slidably installed in the liquid filling seat, and the movable bracket is set toward the support rod. The second roller is installed on the movable bracket, and the movable bracket is abutted against the support rod through the second roller.

[0007] As a preferred solution of the present invention, the first roller is a unidirectional rotating roller.

[0008] As a preferred solution of the present invention, the height of the swing groove decreases from the outer side of the liquid adding seat toward the side of the reaction plate, so that the support rod swings in the swing groove, and a plurality of paddles are evenly distributed at the bottom of the support rod, and the paddles are adapted to the sliding limiter. The support rod swings to drive the paddles and the sliding limiter to separate.

[0009] As a preferred solution of the present invention, a groove is connected to the bottom of the swing slot, the paddle extends into the groove, and a sliding limiter is arranged in the groove to limit the paddle by the sliding limiter.

[0010] As a preferred solution of the present invention, a swing limiter for limiting the swing of the support rod is also provided in the liquid filling seat. The swing limiter includes a swing limiter column and a spring. The swing limiter column is slidably installed in the liquid filling seat. The spring is respectively connected to the liquid filling seat and the swing limiter column to abut the swing limiter column against the support rod.

[0011] As a preferred solution of the present invention, a baffle for marking the liquid droplets in the reaction unit tube is installed on the reaction unit tube, and a lever is connected to one side of the reaction plate to drive the baffle to slide.

[0012] As a preferred solution of the present invention, a rib is provided inside the reaction plate, both sides of the baffle are slidably connected to the rib, the shift rod is swingably mounted on the rib, and the baffle is slidably connected to the middle of the shift rod.

[0013] As a preferred solution of the present invention, a lens and an adjustment component are installed on the base. The lens is arranged below the reaction plate in an adjustable angle. The adjustment component is connected to the lens to adjust the angle between the lens and the reaction plate.

[0014] Compared with the prior art, the present invention has the following beneficial effects: (1) The dropper is supported by a support rod to replace the manual hand-held dripping method, making it easier for the dropper to drip the sample diluent into the reaction unit tube and preventing the sample diluent from coming into contact with the experimenter or dripping outside the reaction unit tube.

[0015] (2) The support rod is limited in movement by a sliding limiter to ensure that the distance the support rod can move each time is consistent with the spacing between adjacent reaction unit tubes, thereby facilitating the dropper to add liquid to the reaction unit tubes in sequence through the support rod, thereby preventing repeated or missed addition of sample diluent to the reaction unit tubes. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a structural diagram of this device.

[0017] Figure 2 It is a schematic diagram of the structure of the device with the base hidden.

[0018] Figure 3 It is a cross-sectional view of the liquid filling seat.

[0019] Figure 4 It is a partial cross-sectional view of the reaction plate.

[0020] Figure 5 It is a cross-sectional view of the reaction unit tube.

[0021] Figure 6 It is a structural diagram of the base.

[0022] Figure 7 This is a schematic diagram of the structure after the lens is hidden in the base.

[0023] Figure 8 It is a diagram of the movement process of the support rod.

[0024] Figure markings: base 1, lens 1-1, adjustment assembly 1-2, fixing plate 1-3, L-shaped chassis 1-4, adjustment screw 1-5, slider 1-6, waist-shaped hole 1-7, clip 1-8, slide 1-9, heating plate 1-10, liquid guide groove 1-11, reaction plate 2, reaction unit tube 2-1, baffle 2-2, lever 2-3, rib 2-4, slide 2-5, through groove 2-6, support rod 3, lever 3-1, positioning groove 3-2, liquid adding seat 4, swing groove 4-1, sliding limiter 4-2, groove 4-3, swing limit column 4-4, spring 4-5, mounting groove 4-6, sliding spring 4-7, locking screw 4-8, directional sliding mechanism 5, first roller 5-1, movable bracket 5-2, second roller 5-3. DETAILED DESCRIPTION

[0025] The present invention will be further described below with reference to the accompanying drawings.

[0026] like Figure 1-Figure 2As shown, a TPPA reaction device includes: a base 1, a reaction plate 2, a support rod 3 and a liquid adding seat 4. The reaction plate 2 is detachably placed on the base 1. A plurality of reaction unit tubes 2-1 are evenly arranged in horizontal and vertical rows on the reaction plate 2. The reaction unit tubes 2-1 are used to perform TPPA reactions. The liquid adding seat 4 is slidably installed on the base 1. The support rod 3 is oriented to the reaction unit tubes 2-1 in different vertical rows by sliding the liquid adding seat 4. A positioning groove 3-2 is provided on the support rod 3. When dripping, the dropper is placed in the positioning groove 3-2 and supported by the positioning groove 3-2, thereby facilitating The dropper drips the sample diluent into the reaction unit tube 2-1 to prevent the sample diluent from coming into contact with the experimenter or dripping outside the reaction unit tube. A swinging groove 4-1 is provided in the liquid adding base 4, and the support rod 3 is swingably and / or movably connected to the liquid adding base 4 through the swinging groove 4-1, that is, the support rod 3 can swing, slide, or swing and slide simultaneously in the swinging groove 4-1. By sliding the support rod 3 in the swinging groove 4-1, the positioning groove 3-2 is made to face different reaction unit tubes 2-1 in the same vertical column, thereby facilitating the dripping of samples into different reaction unit tubes 2-1 through the dropper.

[0027] A directional sliding mechanism 5 and a sliding limiter 4-2 are provided in the swing groove 4-1. The directional sliding mechanism 5 is used to control the unidirectional movement of the support rod 3 toward the reaction plate 2. The sliding limiter 4-2 is used to limit the support rod 3 after movement, ensuring that the distance that the support rod 3 can move each time is consistent with the spacing between adjacent reaction unit tubes 2-1, thereby facilitating the dropper to add liquid to the reaction unit tubes in sequence through the support rod. The support rod 3 includes the following working steps. First, the reaction unit tube 2-1 is dripped with samples through the positioning groove 3-2. The dropper applies a certain pressure on the support rod 3, so that the support rod 3 swings in the swing groove 4-1. At this time, the support rod 3 is separated from the sliding limiter 4-2 to facilitate the translation of the support rod 3, and the positioning groove 3-2 of the support rod 3 is moved to above different reaction unit tubes 2-1. When the positioning groove 3-2 moves to above the downward reaction unit tube 2-1, the support rod 3 and the sliding limiter 4-2 abut, thereby limiting the support rod 3 from moving further. Then, the reaction unit tube 2-1 is dripped with samples through the positioning groove 3-2. This process is repeated, and the entire vertical row of reaction unit tubes 2-1 is dripped with samples once.

[0028] The directional sliding mechanism 5 includes a first roller 5-1 and a movable directional assembly that are relatively arranged, and the first roller 5-1 and the movable directional assembly are in contact with the upper and lower end surfaces of the support rod 3 to ensure that the support rod 3 performs directional sliding, and also ensure that the paddle 3-1 on the support rod 3 is separated from the sliding limiter 4-2 during the swinging process; wherein, the first roller 5-1 is a unidirectional rotating roller, which is installed in the swinging groove 4-1 through a rotating shaft, so that the position of the first roller 5-1 is relatively fixed; the movable directional assembly includes a movable bracket 5 -2, the second roller 5-3, a mounting groove 4-6 connected to the swing groove 4-1 is provided in the liquid adding seat 4, the movable bracket 5-2 is slidably installed in the mounting groove 4-6, and the second roller 5-3 is installed on the movable bracket 5-2. Similarly, the second roller 5-3 is also a one-way rotating roller. A sliding spring 4-7 is provided between the movable bracket 5-2 and the inner wall of the mounting groove 4-6. The sliding spring 4-7 provides a force to the movable bracket 5-2, so that the second roller 5-3 always abuts against the upper end face of the support rod 3.

[0029] As a preferred embodiment, the first roller 5-1 and the second roller 5-3 are used to ensure that the support rod 3 moves only toward one side of the reaction plate 2. Figure 8 As shown, under normal conditions, the contact points of the support rod 3 with the first roller 5-1 and the second roller 5-3 are A and B respectively. When the support rod 3 is swung by the force of the dropper, the support rod 3 swings with point A as the swing center, and the contact point of the support rod 3 and the second roller 5-3 moves from point B to point C. When the support rod 3 is reset by the action of its own weight, since the second roller 5-3 abuts against point C and the second roller 5-3 is a one-way rotating wheel, the support rod 3 is affected by the first roller 5-1 during the reset process, so that the support rod 3 moves toward the reaction point. The plate 2 moves, so that the contact point between the lower end surface of the support rod 3 and the first roller 5-1 moves from point A to point D, so that the support rod 3 moves a certain distance toward the reaction plate 2 during one swinging process, so that the paddle 3-1 and the sliding limiter 4-2 on the support rod 3 can be stably separated, which is convenient for the experimenter to drive the reset support rod 3 to move toward one side of the reaction plate 2 until the next paddle 3-1 on the support rod 3 abuts against the sliding limiter 4-2. At this time, the positioning groove 3-2 moves to above the next reaction unit tube 2-1 to be dripped.

[0030] The height of the swing groove 4-1 decreases from the outer side of the liquid adding seat 4 toward the side of the reaction plate 2, so that the support rod 3 can slide or slide and swing simultaneously in the swing groove 4-1. The first roller 5-1 and the movable directional assembly are respectively arranged on the side of the swing groove 4-1 with a smaller groove height, and the sliding limiter 4-2 is arranged on the side of the swing groove 4-1 with a larger groove height.

[0031] A plurality of paddles 3-1 are evenly arranged at the bottom of the support rod 3. The spacing between the paddles 3-1 is consistent with the spacing between the reaction unit tubes 2-1. The width of the paddle 3-1 is smaller than the width of the support rod 3. In order to prevent the paddle 3-1 from interfering with the bottom surface of the swing groove 4-1, a groove 4-3 is connected to the bottom of the swing groove 4-1, and the paddle 3-1 extends into the groove 4-3. At the same time, in order to facilitate the sliding limiter 4-2 to provide a limiting effect on the support rod 3, the sliding limiter 4-2 is arranged in the groove 4-3, and the paddle 3-1 is limited by the sliding limiter 4-2, thereby realizing the sliding limiter 4-2 limiting the support rod 3.

[0032] In order to facilitate the installation of the first roller 5 - 1 , a roller groove is provided on the side wall of the groove 4 - 3 , and the first roller 5 - 1 is rotatably installed in the roller groove via a rotating shaft.

[0033] like Figure 3 As shown, to prevent the support rod 3 from swinging in the swing groove 4-1 under the action of its own weight, a swing limiter is further provided in the liquid filling seat 4. The swing limiter is arranged above the sliding limiter 4-2 and abuts against the support rod 3, so that the bottom surface of the support rod 3 is in contact with the bottom surface of the swing groove 4-1. This is used to limit the support rod 3 from freely swinging in the swing groove 4-1 and prevent the support rod 3 from separating from the sliding limiter 4-2 in the absence of external force. The swing limiter includes a swing limiter post 4-4 and a spring 4-5. The swing limiter post 4-4 is slidably mounted in the liquid filling seat 4. The spring 4-5 is respectively connected to the liquid filling seat 4 and the swing limiter post 4-4 to abut the swing limiter post 4-4 against the support rod 3.

[0034] like Figure 6-Figure 7 As shown, the base 1 includes a fixed plate 1-3 and an L-shaped chassis 1-4 connected to the bottom of the fixed plate 1-3, and the reaction plate 2 is detachably placed on the fixed plate 1-3. A lens 1-1 and an adjustment component 1-2 are installed on the L-shaped chassis 1-4. The lens 1-1 is arranged below the reaction plate 2 with an adjustable angle. The adjustment component 1-2 is connected to the lens 1-1 to adjust the angle between the lens 1-1 and the reaction plate 2. The adjustment component 1-2 includes an adjusting screw 1-5 and a slider 1-6. The slider 1-6 is slidably mounted on the L-shaped chassis 1-4. The adjusting screw 1-5 is connected to the slider 1-6 to drive the slider 1-6 to slide. One side of the lens 1-1 is in contact with the corner of the L-shaped chassis 1-4. The height of the other side of the lens 1-1 is adjusted by the slider 1-6, thereby changing the angle between the lens 1-1 and the reaction plate 2, so that the experimenter can observe whether the reaction unit tube 2-1 has been added with a sample through the lens 1-1.

[0035] A plurality of waist-shaped holes 1-7 are evenly distributed in the fixing plate 1-3, and a clip 1-8 is provided in the waist-shaped hole 1-7. When the reaction plate 2 is placed, the reaction unit tube 2-1 is inserted into the waist-shaped hole 1-7, and the clip 1-8 abuts against the side wall of the waist-shaped hole 1-7, thereby playing a certain fixing role for the reaction plate 2.

[0036] A slide groove 1-9 is provided on the fixed plate 1-3, and the liquid adding seat 4 is slidably installed on the slide groove 1-9. A locking screw 4-8 is installed on the liquid adding seat 4. The liquid adding seat 4 is locked by the locking screw 4-8 so that the support rod 3 is facing the reaction unit tubes 2-1 on different vertical columns, so that the experimenter can adjust the liquid adding seat 4 conveniently.

[0037] A liquid guide groove 1-11 is provided on the upper end surface of the fixed plate 1-3, and the liquid guide groove 1-11 is used to collect liquid dripping from the reaction plate 2. A heating plate 1-10 is provided at the bottom of the fixed plate 1-3, and the heating plate 1-10 is arranged opposite to the liquid guide groove 1-11, and is used to heat the liquid guide groove 1-11 to kill or reduce the activity of viruses in the liquid in the liquid guide groove 1-11.

[0038] like Figure 4 、 Figure 5 As shown, a baffle 2-2 is mounted on the reaction unit tube 2-1, the width of the baffle 2-2 being less than or equal to the radius of the reaction unit tube 2-1. A lever 2-3 is connected to one side of the reaction plate 2, and the lever 2-3 drives the baffle 2-2 to slide. The sliding movement of the baffle 2-2 on the reaction unit tube 2-1 marks whether the reaction unit tube 2-1 is dripping liquid. That is, before dripping liquid, the baffle 2-2 is located on the side of the reaction unit tube 2-1 close to the liquid adding seat 4. After dripping liquid, the baffle 2-2 is located on the side of the reaction unit tube 2-1 away from the liquid adding seat 4. Preferably, the upper end of the lever 2-3 is higher than the upper end surface of the reaction plate 2, so that the lever 2-3 can be driven to swing by the paddle 3-1 of the support rod 3. It should be noted that a certain gap is provided between the upper end surface of the lever 2-3 and the lower end surface of the support rod 3 to ensure that the support rod 3 can swing.

[0039] A rib 2-4 is provided in the reaction plate 2, and a slide groove 2-5 is provided on the rib 2-4. The two sides of the baffle 2-2 are slidably connected to the ribs 2-4 located on both sides of the reaction unit tube 2-1 through the slide groove 2-5. A through groove 2-6 is provided on the side wall of the reaction unit tube 2-1, and the through groove 2-6 corresponds to the slide groove 2-5, so that the baffle 2-2 can move above the reaction unit tube 2-1. The lever 2-3 is swingably mounted on the rib 2-4, and the baffle 2-2 is slidably connected to the middle part of the lever 2-3. The rib 2-4 increases the strength of the reaction plate 2 and provides a guide for the sliding of the baffle 2-2.

[0040] The working steps of this device are as follows: (1) Place the reaction plate 2 on the base 1; (2) Insert the support rod 3 into the liquid adding seat 4 and adjust the liquid adding seat 4 so that the support rod 3 faces the reaction unit tube 2-1 in a vertical row on the reaction plate 2; (3) The experimenter moves the support rod 3 until the support rod 3 cannot move (the paddle 3-1 contacts the sliding limiter 4-2); (4) The experimenter places the dropper against the support rod 3 and adds liquid to the reaction unit tube 2-1 through the support rod 3. After the dripping is completed, the experimenter applies a force to the support rod 3 through the dropper, causing the support rod 3 to swing, so that the paddle 3-1 and the sliding limiter 4-2 are separated; (5) Repeat steps (3) and (4) until the dripping of the reaction unit tubes 2-1 in this row is completed, and then adjust the liquid adding seat 4 so that the support rod 3 faces the reaction unit tubes 2-1 in the next vertical row.

[0041] The above description of the disclosed embodiments will enable one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be readily apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not limited to the embodiments shown herein, but is to be embodied in the widest possible manner consistent with the principles and novel features disclosed herein.

[0042] Although this article uses more terms corresponding to the figure marks in the figures, it does not exclude the possibility of using other terms; these terms are used only to more conveniently describe and explain the essence of the present invention; interpreting them as any additional restrictions is contrary to the spirit of the present invention.

Claims

1. A TPPA reaction device, characterized in that: include: Base (1); A reaction plate (2) having a plurality of reaction unit tubes (2-1) uniformly arranged in horizontal and vertical rows, and the reaction plate (2) is detachably placed on the base (1); A support rod (3) for supporting the dropper; A liquid adding seat (4) is slidably mounted on a base (1); a support rod (3) is slidably mounted on the liquid adding seat (4); the support rod (3) is slidably mounted on the liquid adding seat (4); the liquid adding seat (4) is slidably moved so that the support rod (3) faces the reaction unit tubes (2-1) in different vertical rows; a swinging groove (4-1) is provided in the liquid adding seat (4); the support rod (3) is swingably and / or movably connected to the swinging groove (4-1); a directional sliding mechanism (5) for controlling the unidirectional movement of the support rod (3) and a sliding stopper (4-2) for limiting the support rod (3) after movement are provided in the swinging groove (4-1); the support rod (3) is swung in the swinging groove (4-1) to separate the support rod (3) from the sliding stopper (4-2); and the support rod (3) is moved to above different reaction unit tubes (2-1) by sliding the support rod (3) in the swinging groove (4-1).

2. A TPPA reaction device according to claim 1, characterized in that: The directional sliding mechanism (5) comprises a first roller (5-1) which is arranged opposite to and abuts against the support rod (3), and a movable directional component, wherein the first roller (5-1) is rotatably mounted in the swing groove (4-1), and the movable directional component is slidably mounted in the swing groove (4-1).

3. A TPPA reaction device according to claim 2, characterized in that: The movable orientation component comprises a movable bracket (5-2) and a second roller (5-3) having a unidirectional rotation function; the movable bracket (5-2) is slidably mounted in a liquid adding seat (4), and the movable bracket (5-2) is arranged toward the support rod (3); the second roller (5-3) is mounted on the movable bracket (5-2), and the movable bracket (5-2) is brought into contact with the support rod (3) through the second roller (5-3).

4. A TPPA reaction device according to claim 2, characterized in that: The first roller (5-1) is a unidirectional rotating roller.

5. A TPPA reaction device according to claim 1, characterized in that: The height of the swing groove (4-1) decreases from the outer side of the liquid adding seat (4) toward the side of the reaction plate (2), so that the support rod (3) swings in the swing groove (4-1); a plurality of paddles (3-1) are evenly arranged at the bottom of the support rod (3); the paddles (3-1) are adapted to the sliding limiter (4-2); and the paddles (3-1) and the sliding limiter (4-2) are driven to separate by the swinging of the support rod (3).

6. A TPPA reaction device according to claim 5, characterized in that: The bottom of the swing slot (4-1) is connected to a groove (4-3), the paddle (3-1) extends into the groove (4-3), and the sliding limiter (4-2) is arranged in the groove (4-3) so as to limit the paddle (3-1) through the sliding limiter (4-2).

7. A TPPA reaction device according to claim 5, characterized in that: The liquid adding seat (4) is also provided with a swing limiter for limiting the swing of the support rod (3), the swing limiter comprising a swing limiter column (4-4) and a spring (4-5), the swing limiter column (4-4) being slidably mounted in the liquid adding seat (4), and the spring (4-5) being respectively connected to the liquid adding seat (4) and the swing limiter column (4-4) so ​​as to abut the swing limiter column (4-4) against the support rod (3).

8. A TPPA reaction device according to claim 1, characterized in that: The reaction unit tube (2-1) is provided with a baffle (2-2) for marking liquid drops in the reaction unit tube (2-1), and a lever (2-3) is connected to one side of the reaction plate (2), and the baffle (2-2) is driven to slide by the lever (2-3).

9. A TPPA reaction device according to claim 8, characterized in that: The reaction plate (2) is provided with a rib plate (2-4), both sides of the baffle plate (2-2) are respectively slidably connected to the rib plate (2-4), the lever (2-3) is swingably mounted on the rib plate (2-4), and the baffle plate (2-2) is slidably connected to the middle of the lever (2-3).

10. A TPPA reaction device according to claim 1, characterized in that: The base (1) is mounted with a lens (1-1) and an adjustment component (1-2); the lens (1-1) is arranged below the reaction plate (2) in an angle-adjustable manner; the adjustment component (1-2) is connected to the lens (1-1) to adjust the angle between the lens (1-1) and the reaction plate (2).

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