Composition with effects of dispelling effects of alcohol and protecting liver as well as preparation method and application of composition

Abstract

The invention provides a composition with effects of dispelling the effects of alcohol and protecting the liver. The composition is prepared from the following raw materials in parts by weight: 0.15-0.35 part of total flavonoids of kudzuvine roots and 0.15-0.30 part of a dark plum aqueous extract. The invention also provides a preparation method and application of the composition. According to the invention, the total flavonoids of the kudzuvine root and the aqueous extract of the dark plum fruit are compatible and can play a synergistic effect under a specific proportioning condition, especially when the compatibility ratio of the total flavonoids of the kudzuvine root to the polysaccharide of the kudzuvine root to the aqueous extract of the dark plum fruit is 0.15: 0.15: 0.15, the liver protection effect is most obvious, and compared with the direct use of the aqueous extract of the kudzuvine root and the aqueous extract of the dark plum fruit, the application dosage is obviously reduced, and the application cost is reduced. And a new choice is provided for clinic.

Description

Technical Field

[0001] The invention relates to a composition with alcohol sobering and liver protecting functions, a preparation method and application thereof, and belongs to the field of medicines or health foods. Background Art

[0002] Alcoholic liver disease (ALD) refers to a liver disease caused by excessive drinking. The initial symptoms are often fatty liver, which can then develop into alcoholic hepatitis, liver fibrosis, and cirrhosis. In severe cases, it can even deteriorate into liver failure and liver cancer. [1] Studies have found that alcoholic liver damage is mainly caused by the fact that alcohol produces a large amount of reactive oxygen species (ROS) through various pathways during metabolism, which reduces the activity of antioxidant enzymes and increases lipid peroxidation, thereby destroying the body's redox balance and mitochondrial function, inducing oxidative stress and lipid metabolism disorders, and finally leading to liver damage. [3] Early clinical treatments for alcoholic liver damage mainly include quitting drinking, nutritional support, and drug-assisted therapy, but long-term use of drugs will produce some adverse reactions. Therefore, the development of alcohol-detoxifying and liver-protecting products with good effects and low toxicity and side effects has broad market prospects.

[0003] As people pay more and more attention to health issues in recent years, the concept of health care is increasingly recognized by people. Therefore, the health care market developed with Chinese medicinal materials for both medicinal and edible purposes as natural raw materials has great development potential, among which the research and development of various products deserves attention. However, the first problem in development is the taste problem. Most Chinese medicines are bitter, so it is very necessary to develop Chinese medicine compositions with good taste. At present, the market for Chinese medicine products is very large, but products containing medicinal ingredients only account for a small part, and most of them are widely circulated among the people, such as Chinese medicine beverages such as sour plum soup and five-flower tea. Among the hundreds of Chinese medicines, only a part of the detoxification and heat-clearing Chinese medicines are really bitter, such as Huangling, Dahuang, Chuanhuanglian, Banlangen, and Chuanxinlian. It is feasible to develop Chinese medicine compositions with good taste and no bitterness.

[0004] In my country, kudzu root has a long history of being used to sober up. Nowadays, its application research in the treatment of alcoholism has received increasing attention. Pueraria root is the dried root of perennial deciduous vine plant kudzu or sweet kudzu vine of the genus kudzu of the leguminous family. It tastes sweet, is pungent and cool in nature, and enters the spleen and stomach meridians. It has the functions of relieving muscles and fever, clearing rashes, promoting fluid and relieving thirst, eliminating restlessness, raising yang and stopping diarrhea, promoting blood circulation and treating sores, and sobering up. Pueraria root was first recorded in "Shennong's Herbal Classic" and listed as a medium-grade product. It has been recorded in herbal books of all dynasties and is one of the commonly used Chinese medicines. Pueraria root was used to "detoxify alcohol" in the Tang Dynasty. For example, "Qianjin Yaofang" used fresh kudzu root juice to treat those who were drunk and unconscious. Therefore, it can be seen that kudzu root has a symptomatic effect on sobering up.

[0005] Through the extraction, separation and identification of the main components of Pueraria root, it was found that the main chemical components of Pueraria root are mainly the following categories: isoflavones (puerarin, daidzein, daidzin, etc.), puerarin glycosides (puerarin A, B, C), triterpenoid glycosides, alkaloids and other compounds. At present, isoflavones are the most concerned and are also its main active ingredients. [5] In recent years, a large number of studies have been conducted at home and abroad on the pharmacological effects of total flavonoids of Pueraria lobata and its monomer component puerarin, and they have been widely valued and applied in clinical practice. [6,7] . Numerous studies have shown that Pueraria root extracts can inhibit alcohol absorption in mice and rats, accelerate alcohol metabolism, increase alcohol tolerance, reduce mortality and reduce liver damage. Among them, Pueraria root total flavonoids are the main active ingredients. Its mechanism of action may be to delay gastric emptying, slow down the rate of alcohol absorption, and reduce intestinal wall permeability, thereby reducing alcohol damage to the central nervous system and liver. Polysaccharides, as one of the main active ingredients of Pueraria root, have multiple biological activities. [8,9] . Researchers have confirmed that Pueraria polysaccharide not only effectively prolongs the incubation period of drunkenness and shortens the sobering time, but also can significantly fight against liver pathological damage and biochemical changes caused by alcohol, playing a role in liver protection. As a prebiotic, Pueraria polysaccharide has a regulatory effect on intestinal flora, can regulate intestinal flora diversity, improve intestinal flora structure, and can also significantly reduce branched-chain fatty acids in the intestine, which is beneficial to the health of the body.

[0006] Wumei, also known as plum fruit, blackberry, smoked plum, and orange plum meat, is the dried nearly mature fruit of the plum plant of the Rosaceae family. It was first seen in "Shennong's Herbal Classic" and has a long history of medicinal use. Wumei is mainly distributed throughout my country, mainly in the south of the Yangtze River. It tastes sour and astringent, and is neutral in nature. It belongs to the liver, spleen, lung, and large intestine meridians. It has the effect of astringing the lungs and astringing the intestines. It is mainly used to treat chronic coughs, chronic diarrhea, and dysentery caused by lung deficiency. Traditional Chinese medicine believes that it has the effect of harmonizing liver qi and nourishing liver blood, can refresh the mind, promote body fluid and quench thirst, and can also help restore vitality to muscle and vascular tissues.

[10] Modern research has found that black plum has antioxidant, antibacterial, antitussive, sedative, antiviral, and anti-tumor effects

[11] . The antioxidant effect of black plum is mainly related to the organic acids in it. The organic acid components in black plum can significantly reduce cell apoptosis, increase cell survival rate, and the activity of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT)

[12] . At the same time, black plum, as a substance with medicinal and edible properties in my country, is a traditional Chinese medicine with a long research background. The organic acid content in black plum is very rich, which can effectively inhibit the production of lactic acid, reduce fatigue, and remove harmful substances that cause vascular aging

[13] . Studies have shown that black plum can be used as a beverage and other food in addition to being used as a medicine. At the same time, soaking black plum in water after drinking can effectively relieve the symptoms of drunkenness, protect the liver, and reduce liver damage. It can reduce glutamic transaminase and jaundice, and improve symptoms and signs in patients with hepatitis.

[0007] In addition, the mechanism of alcohol detoxification and liver protection is complex, mainly involving the following aspects:

[0008] 1. Promote alcohol metabolism: By increasing the activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH), it accelerates the decomposition of alcohol and its metabolite acetaldehyde, reducing damage to the liver.

[0009] 2. Antioxidant effect: A large number of free radicals are produced during alcohol metabolism. Alcohol-detoxifying and liver-protecting substances can protect the liver by increasing the activity of antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT), removing free radicals and reducing oxidative stress.

[0010] 3. Anti-inflammatory effect: Alcohol can cause inflammatory response in the liver. Alcohol-detoxifying and liver-protecting substances can reduce the levels of inflammatory factors such as tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6), thereby alleviating liver inflammatory damage.

[0011] 4. Protect liver cells: Some active ingredients can stabilize liver cell membranes, inhibit liver cell apoptosis, and promote liver cell regeneration, thereby protecting liver structure and function.

[0012] 5. Regulate intestinal flora: Dietary fiber increases the abundance of acid-producing Bacteroides in the intestine, reduces the imbalance and toxicity of ammonia metabolism caused by alcohol, and thus alleviates liver damage.

[0013] 6. Reduce the impact of alcohol on the brain: Studies have found that astrocytes in the brain contain ALDH2, which can metabolize alcohol and reduce the damage of alcohol to brain function.

[0014] 7. Promote liver detoxification: Some alcohol-detoxifying and liver-protecting substances can enhance the liver's detoxification ability and accelerate the decomposition and excretion of alcohol.

[0015] 8. Regulate lipid metabolism: Alcohol can cause fat accumulation in the liver. Alcohol-detoxifying and liver-protecting substances can reduce liver fatty degeneration by regulating lipid metabolism. (Zhuo Xinxin, et al., Research Progress on Alcohol-detoxifying Mechanism and Alcohol-detoxifying Products, China Brewing, Vol. 43, No. 2, 2024).

[0016] Therefore, the mechanism by which the raw materials play their role in sobering up and protecting the liver is not a single one, but a complex system.

[0017] CN202310572874.3, Invention Name: A composition with alcohol sobering and liver protection effects, and its preparation method and use, provides a composition with alcohol sobering and liver protection effects, which is prepared from the following raw materials in weight ratio: 1-2 parts of Pueraria root and 1-2 parts of black plum. The composition of the present invention has the effects of nourishing the liver and spleen, sobering up and protecting the liver, and has a good protective effect on rat liver damage caused by alcohol, especially when Pueraria root: black plum = 1:1, the amount of raw materials used is the least, the effect is the best, and it has a significant synergistic effect. However, this prescription is a direct water extraction, and the dosage is large. Alcohol sobering and liver protection drugs generally refer to those that can improve liver function, promote liver cell regeneration, and enhance the liver's detoxification function, aiming to improve liver pathology and improve liver function. However, these drugs themselves also need to be metabolized by the liver. If used improperly, they will not only fail to protect the liver, but may increase the metabolic burden of the liver. Summary of the invention

[0018] The technical scheme of the present invention is to provide a composition with alcohol sobering and liver protecting effects, and a preparation method and use thereof.

[0019] The present invention provides a composition with alcohol sobering and liver protecting effects, which is prepared from the following raw materials in weight ratio:

[0020] 0.15-0.35 parts of total flavonoids of Pueraria root and 0.15-0.30 parts of water extract of black plum.

[0021] More preferably, it is prepared from the following raw materials in weight ratio:

[0022] 0.18 parts of total flavonoids from Pueraria root and 0.27 parts of water extract from black plum.

[0023] Furthermore, it is prepared from the following raw materials in weight ratio:

[0024] 0.15-0.35 parts of total flavonoids of kudzu root, 0.15-0.30 parts of kudzu root polysaccharides, and 0.15-0.30 parts of black plum water extract.

[0025] More preferably, it is prepared from the following raw materials in weight ratio:

[0026] 0.15 parts of total flavonoids of kudzu root, 0.15 parts of kudzu root polysaccharides, and 0.15 parts of ebony water extract.

[0027] The composition of the present invention is prepared from the raw materials as active ingredients, and is added with auxiliary materials or auxiliary ingredients acceptable in pharmacy or health food to form a common preparation.

[0028] Wherein, the preparation is oral liquid, tablet, capsule, pill, granule.

[0029] The present invention provides a method for preparing the composition having the effects of sobering up and protecting the liver, which comprises the following steps:

[0030] a. Weigh the raw materials of each weight ratio;

[0031] b. Mixing and adding excipients or auxiliary ingredients acceptable in pharmacy or health food to prepare commonly used preparations.

[0032] The present invention also provides use of the composition in preparing a medicine for treating alcoholic liver damage.

[0033] The present invention also provides the use of the composition in preparing health food having auxiliary protective effect on chemical liver damage.

[0034] The present invention combines total flavonoids of kudzu root and water extract of black plum, and can play a synergistic role under specific ratio conditions, especially when the ratio of total flavonoids of kudzu root: kudzu root polysaccharide: black plum water extract is 0.15:0.15:0.15, the liver protection effect is most obvious. Compared with directly using the water extracts of kudzu root and black plum, the dosage of application is significantly reduced, which provides a new choice for clinical practice. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 Rutin standard curve;

[0036] Figure 2 Anhydrous glucose standard curve;

[0037] Figure 3 Changes of rat liver pathology and liver function indicators;

[0038] Figure 4 HE staining results (A: blank group, B: model group, C: kudzu root polysaccharide group, D: ebony water extract group, E: combination group 1, F: combination group 2, G: combination group 3, H: combination group 4, I: combination group 5, J: kudzu root total flavonoids group, K: combination group 6, L: positive control group). DETAILED DESCRIPTION

[0039] The raw materials of pueraria root total flavonoids, pueraria root polysaccharide and ebony root water extract used in the present invention can be commercially available or prepared by the following method:

[0040] 1. Total flavonoids from Pueraria root

[0041] Based on the research on microwave extraction technology of total flavonoids from Pueraria lobata and its antioxidant activity by Liu Yushi et al., microwave extraction of total flavonoids from Pueraria lobata was carried out.

[0042] Take about 0.5g of Pueraria powder (sieved), weigh it accurately, put it in a microwave digestion tank, add different volume fractions of extraction solvent according to different material-liquid ratios, seal it, put it in a microwave digestion instrument, and perform microwave extraction at 60℃ with different microwave powers and times.

[14] .

[0043] The total flavonoids of Pueraria root were calculated by puerarin, which had the highest content. Puerarin (C 21 H 20 O9) content shall not be less than 2.4%.

[0044] The determination of total flavonoids in Pueraria root was based on Zhang Guodong et al.

[15] In the study on the optimization of water extraction process of total flavonoids in Pueraria root, the content of total flavonoids in Pueraria root was determined by aluminum nitrate colorimetric method.

[0045] 2. Pueraria polysaccharide

[0046] Based on the optimization of the pueraria polysaccharide extraction process by Pei Lixin et al., pueraria polysaccharide was extracted from pueraria.

[0047] Weigh 50.00g of Pueraria root powder, place in a round-bottom flask, add 250mL of petroleum ether, reflux extract for 1h, filter, evaporate the residue solvent, and place in a flask. Repeat the operation once, add distilled water, reflux extract, filter while hot, concentrate the filtrate to 50mL, add anhydrous ethanol, stand at a certain temperature, filter, wash the precipitate with anhydrous ethanol and acetone 8 times in sequence, 5mL each time, to obtain the total polysaccharide component, dry at 60℃, and weigh.

[16] The content of pueraria polysaccharide was determined according to Zeng Ming et al.

[17] In the comparative determination of the Pueraria polysaccharide content, the phenol-concentrated sulfuric acid spectrophotometry was used to determine the Pueraria polysaccharide content.

[0048] 3. Plum water extract

[0049] Zang Lei et al. prepared the black plum water extract in the antibacterial effect of black plum water extract on Staphylococcus aureus. According to this document, 20g of black plum powder was placed in a 500mL flask, 200mL of distilled water was added, soaked for 0.5h, boiled and refluxed for 1.5h, filtered, and the extract was obtained. The residue was filtered, and 100mL of distilled water was added, boiled and refluxed again for 45min and filtered, the extract was combined, concentrated, stored at 4℃, and set aside.

[0050] The main component of black plum is organic acid, among which citric acid has the highest content. According to the 2020 edition of the "Chinese Pharmacopoeia" measured by high performance liquid chromatography, the citric acid content (C6H8O7) must not be less than 12.0%.

[0051] The determination of the content of organic acid in black plum was carried out based on the extraction process and antibacterial activity of organic acid in black plum by Zhang Dandan et al. using potentiometric titration.

[0052] The beneficial effects of the present invention are demonstrated by pharmacodynamic tests as follows.

[0053] Experimental Example 1 Effect of the present invention's composition of Pueraria root combined with Prunus mume on rats with alcoholic liver injury

[0054] 1. Experimental Materials and Reagents

[0055] 1.1 Drugs and Reagents

[0056] Total flavonoids from kudzu root, kudzu root polysaccharide, water extract of black plum; AST biochemical kit, ALT biochemical kit, ALDH kit, anhydrous ethanol;

[0057] ① Determination results of total flavonoids in Pueraria root

[0058] After determination, the absorbance of the Pueraria root samples was 0.353, 0.355, and 0.356. After substituting into the standard curve ( Figure 1 ), the sample content of total flavonoids in the Pueraria root sample was calculated to be 79.44 mg / g.

[0059] ② Determination results of polysaccharides in Pueraria root

[0060] After determination, the absorbance of the Pueraria root samples was 0.321, 0.320, and 0.318. After substituting into the standard curve ( Figure 2 ), the sample content of polysaccharides in the Pueraria root sample was calculated to be 51.44 mg / g.

[0061] ③ Determination results of total organic acids in black plum

[0062] During the titration, the volume of NaoH consumed was 2.38 ml. Substituting it into the formula, the total organic acid content in black plum was calculated to be 25.38%.

[0063] 1.2 Experimental instruments

[0064] Analytical balance, CNC ultrasonic cleaner, desktop centrifuge, microplate reader, paraffin embedding machine, paraffin slicer, pathology slicer

[0065] 1.3 Experimental animals

[0066] Male Sprague-Dawley clean-grade rats (180-220) g, certificate number: Animal License No. SCXK(Chuan)2022-030, were fed adaptively for 1 week under experimental conditions (temperature 25±2°C, relative humidity 50±5%). This study was approved by the Animal Ethics Committee of Shaanxi University of Chinese Medicine (approval number SUCMDL20231031004).

[0067] 2 Experimental methods

[0068] 2.1 Establishment of rat alcoholic liver injury model and grouping and drug administration

[0069] After 3 days of adaptive feeding, healthy SPF SD rats were randomly divided into 8 groups, with 8 rats in each group. They were blank group, model group, pueraria root total flavonoids group (0.45 g / kg), pueraria root polysaccharide group (0.45 g / kg), black plum water extract group (0.45 g / kg), pueraria root total flavonoids black plum water extract compatibility group 1 (0.30 g / kg pueraria root total flavonoids + 0.15 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 2 (0.27 g / kg pueraria root total flavonoids + 0.18 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 3 (0.225 g / kg pueraria root total flavonoids + 0.225 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 4 (0.225 g / kg pueraria root total flavonoids + 0.225 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 5 (0.225 g / kg pueraria root total flavonoids + 0.225 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 6 (0.225 g / kg pueraria root total flavonoids + 0.225 g / kg black plum water extract), pueraria root total flavonoids black plum water extract compatibility group 7 (0.225 g / kg pueraria root total flavonoids + 0.225 g / kg black plum water extract). Plum water extract compatibility group 4 (0.18g / kg total flavonoids of kudzu root + 0.27g / kg water extract of black plum), total flavonoids of kudzu root + black plum water extract compatibility group 5 (0.15g / kg total flavonoids of kudzu root + 0.30g / kg water extract of black plum), total flavonoids of kudzu root + kudzu root polysaccharide + black plum water extract compatibility group 6 (0.15g / kg total flavonoids of kudzu root + 0.15g / kg kudzu root polysaccharide + 0.15g / kg water extract of black plum), positive control group (according to the extract prepared in Example 1 of patent application number CN202310572874.3, wherein the original drug amount of kudzu root and black plum is 1.8g / kg). Each group was given 0.9% saline or drug by gavage, and 56-degree liquor was given at a dosage of 10mL / kg 1h later for 14 consecutive days.

[0070] Among them, the positive control group is described in the example in the specification of patent application number CN202310572874.3, the total dosage of Pueraria lobata and Prunus mume is 1.8g-2.8g / kg rat, the lowest dose group is 1.8g / kg, and the extract dosage is 0.6g extract / kg rat according to the extract recovery rate. Its dosage is significantly higher than the dosage shown in formula 1-6.

[0071] 2.2 Detection of physiological and biochemical indices in rats

[0072] One hour after the last drug infusion, the animals were anesthetized and blood was collected from the abdominal aorta. The blood was separated after centrifugation at 4°C and 5000 r / min for 15 min. The liver was removed and the blood on the surface was washed with pre-cooled saline. The surface water was dried with filter paper. The liver was weighed and the liver index was calculated. The liver index calculation formula is as follows:

[0073] Liver index (%) = liver mass (g) / body mass (g) * 100%

[0074] According to the kit instructions, the ALT and AST levels in serum were determined. 0.2 g of left liver tissue was added with 9 times the amount of 0.9% saline to make a 10% liver tissue homogenate. The supernatant was collected for later use at 4°C and 12,000 r / min for centrifugation. ELISA kit was used to determine the ALDH activity in liver tissue.

[0075] The liver tissue was fixed with 10% neutral paraformaldehyde for 24 h and then transferred to 70% alcohol solution for dehydration for 24 h. After conventional paraffin embedding, sections with a thickness of about 4 μm were prepared. 10 Dewaxing and dehydration with gradient alcohol (95%, 90%, 80%, 75%) were performed; hematoxylin staining was performed; hydrochloric acid alcohol differentiation was performed; 0.5% eosin staining was followed by conventional gradient alcohol (75%, 80%, 90%, 95%) dehydration, followed by 100% ethanol (10 min) and 100% alcohol (10 min); finally, the sections were transparentized with xylene and sealed with neutral gum, and different fields of view were selected under a Nikon optical microscope Eclipse Ci- to photograph and analyze the pathological changes of liver tissue.

[0076] 3. Experimental Results

[0077] The test results of the kit are shown in Figure 3 ,Depend on Figure 3 It can be seen that the liver index of the model group (Model) was significantly higher than that of the blank group (Control), and P < 0.05, which was statistically significant. After administration, the liver index of each group decreased to varying degrees. The relevant pathological and biochemical indicators were analyzed. The results showed that when the total flavonoids of Pueraria root + black plum extract group 4 and the total flavonoids of Pueraria root + Pueraria root polysaccharide + black plum water extract group 6 were combined, the levels of ALT and AST in the serum were significantly reduced. At the same time, the activity of acetaldehyde dehydrogenase ALDH could be significantly increased. It was preliminarily judged that the total flavonoids of Pueraria root and

[0078] The combination of black plum water extract has a good protective effect on alcohol-induced liver damage in rats.

[0079] Table 1 Differences in liver function indicators in rats

[0080]

[0081]

[0082] Note: Compared with the blank group, ### p<0.005; compared with the model group, * p<0.05, ** p<0.01.

[0083] HE staining results are shown in Figure 4As shown in Figure A, the liver cell structure of the blank group was full, and no obvious degeneration such as edema, shrinkage, necrosis, etc. of the liver cells was observed, and no obvious inflammatory cell infiltration was observed in the tissue; the overall structure of the liver tissue in the model group B was slightly abnormal, and more liver cells were slightly edematous, and the liver sinusoids were obviously congested and dilated, and the tissue had more inflammatory cell infiltration; the overall structure of the liver tissue in the pueraria polysaccharide group C, the ebony water extract group D, and the pueraria total flavonoids group J was loose, and a small number of liver cells were slightly edematous, and a small number of inflammatory cells were infiltrated in the tissue; the pueraria total flavonoids and ebony water extract compatibility group 1E, the pueraria total flavonoids and ebony water extract compatibility group 2F, the pueraria total flavonoids and ebony water extract compatibility group 3G, and the pueraria total flavonoids and ebony water extract compatibility group 5I showed that the overall structure of each tissue was basically normal, the liver cell structure was loose, the liver sinusoids were arranged radially along the central vein, and no obvious inflammatory cell infiltration was observed in the tissue. The overall structure of the liver tissue in the Pueraria lobata total flavonoids + Pueraria lobata polysaccharides + Prunus mume water extract combination group 6K, the positive drug group L, and the Pueraria lobata total flavonoids and Prunus mume water extract combination group 4H was basically normal, the hepatocyte structure was plump, the central vein was clearly visible, the hepatic sinusoids were arranged radially along the central vein, and no obvious inflammatory cell infiltration was observed in the tissue.

[0084] Discussion

[0085] In this experiment, alcohol gavage was used to replicate a rat model of alcoholic liver injury, and the protective effects of total flavonoids of Pueraria root, Pueraria polysaccharides, black plum water extract and different combinations on the liver were observed. Through the monitoring and analysis of relevant biochemical indicators in rat serum and liver tissue, it was found that the liver pathological indicators of the experimental groups of each combination were significantly decreased compared with the model group, indicating that different combinations of Pueraria root and black plum have a certain protective effect on the liver. Among them, when the ratio of total flavonoids of Pueraria root and black plum water extract was 0.18:0.27, the liver protection effect was more obvious than that of other combination groups. When the ratio of total flavonoids of Pueraria root + Pueraria polysaccharides + black plum water extract was 0.15:0.15:0.15, the liver protection effect was most obvious. Compared with the original drug extract, the dosage was significantly reduced, and the efficacy was significantly improved, especially for the ALT index, there was a significant difference, and the ALDH content in the liver tissue was significantly higher than that in the model group, which provided a theoretical basis for the further development of anti-alcoholic liver injury drugs and related compositions.

[0086] As pure natural plant raw materials with medicinal and edible properties, Pueraria root and black plum have the characteristics of small toxic side effects, low price and obvious effects. They have unique advantages in the development of modern hangover products. Hangover products developed with them as the main raw materials will surely usher in a broad market prospect. At present, the product development and utilization of Pueraria root and black plum are mainly focused on the development of hangover products with different forms and formulas, while there is little research on their effective ingredients and mechanism of action. In the subsequent product development process, this should be further strengthened in order to be more targeted in the research and development of hangover products.

[0087] References

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Claims

1. A composition having the effects of sobering up and protecting the liver, characterized in that: It is prepared from the following raw materials in weight ratio: 0.15-0.35 parts of total flavonoids of Pueraria root and 0.15-0.30 parts of water extract of black plum.

2. The composition having alcohol sobering and liver protecting effects according to claim 1, characterized in that: It is prepared from the following raw materials in weight ratio: 0.18 parts of total flavonoids from Pueraria root and 0.27 parts of water extract from black plum.

3. The composition having the effect of sobering up and protecting the liver according to claim 1 or 2, characterized in that: It is prepared from the following raw materials in weight ratio: 0.15-0.35 parts of total flavonoids of kudzu root, 0.15-0.30 parts of kudzu root polysaccharides, and 0.15-0.30 parts of black plum water extract.

4. The composition having alcohol sobering and liver protecting effects according to claim 3, characterized in that: It is prepared from the following raw materials in weight ratio: 0.15 parts of total flavonoids of kudzu root, 0.15 parts of kudzu root polysaccharides, and 0.15 parts of ebony water extract.

5. The composition having the effects of sobering up and protecting the liver according to any one of claims 1 to 4, characterized in that: The raw material is used as an active ingredient, and adjuvants or auxiliary ingredients acceptable in pharmacy or health food are added to prepare a common preparation.

6. The composition having the effects of sobering up and protecting the liver according to claim 5, characterized in that: The preparation is oral liquid, tablet, capsule, pill or granule.

7. A method for preparing the composition having alcohol sobering and liver protecting effects according to any one of claims 1 to 6, characterized in that: It includes the following steps: a. Weigh the raw materials of each weight ratio; b. Mixing and adding excipients or auxiliary ingredients acceptable in pharmacy or health food to prepare commonly used preparations.

8. Use of the composition according to any one of claims 1 to 6 in the preparation of a medicament for treating alcoholic liver damage.

9. Use of the composition according to any one of claims 1 to 6 in the preparation of a health food having an auxiliary protective effect on chemical liver damage.

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