Red rhododendron flower color regulating agent as well as preparation method and use method of red rhododendron flower color regulating agent

Abstract

The invention discloses a rhododendron red flower color regulating agent and a preparation and use method thereof. The rhododendron red flower color regulating agent comprises an acid buffer solution, and ammonium ferrous sulfate hexahydrate [Fe (NH4) 2. (SO4) 2. 6H2O], a Fe < 2 + > slow-release agent, saccharides and a span-80 surfactant are dissolved in the acid buffer solution. According to the method, the effect of regulating and controlling the synthesis speed and the synthesis amount of the rhododendron safflower pigment can be achieved, so that potted rhododendron flowers have different colors, and the characteristic of color diversification is presented. The rhododendron red flower color regulating agent is wide in raw material source, low in cost and beneficial to popularization and application.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a rhododendron red flower color regulator and a preparation and use method thereof. Background Art

[0002] Rhododendron has extremely high ornamental value and is widely used in landscaping. Potted azalea in particular has become an important indoor beautification item. The cultivation of azalea flower color and the extension of the flower blooming period are key technologies to improve the quality of potted azalea.

[0003] Rhododendron plants are widely distributed in my country and have rich flower colors, but the flowering period of rhododendrons grown in the middle and lower reaches of the Yangtze River is limited to spring and the blooming period is short. In recent years, some new varieties of rhododendrons have been obtained through gene editing technology, such as Anku Rhododendron and Four Seasons Rhododendron. They have the characteristics of continuously growing buds and blooming flowers in spring, autumn and winter, bringing new business opportunities to the rhododendron market. However, these new varieties of rhododendrons have complex soil composition in the middle and lower reaches of the Yangtze River, heavy rain in spring, insufficient light in mountainous areas, and climate change. As a result, the growth of Anku Rhododendron and Four Seasons Rhododendron is unstable, the color of flowers is not regulated, and the life span of flowers is short, which affects the commercial prospects of large-scale promotion and planting. Therefore, it is necessary to explore the biosynthesis mechanism of anthocyanins in this type of rhododendron, and then regulate the color of potted rhododendrons and extend the life span of rhododendron flowers.

[0004] Existing patents mainly cultivate the color of plants in the aspects of fruits and leaves. For example, patents 201010596941.8 and 201410766875.2 are color enhancers that promote the coloring of various fruits such as apples, grapes, cherries, dates, tomatoes and peppers; patent 201910142738.4 is a color enhancer that makes red maple sprout, grow and fall leaves; patent 201711090397.8 is a color enhancer that makes red maple leaves redder. None of these patents involve the color regulation of azalea flowers.

[0005] As the Anku azalea and the Four Seasons azalea have the ability to continuously grow buds and bloom flowers in three seasons, how to achieve the regulation of flower color, extend the flower opening period, and further improve the quality of azalea is a task of great significance. Patent 201710689221.8 is related to a flower color enhancer. This patent does not extend the color retention period of living flowers, nor does it involve how to regulate the synthesis of azalea cyanine.

[0006] Potted azaleas are very ornamental and have great value in beautifying the indoor environment. As potted azaleas placed indoors, their advantage is that the soil, climate, temperature and humidity and other environmental factors of the growing village are controllable, but there are also problems such as insufficient light affecting the synthesis of chlorogenic acid glycosides and leaf growth of azaleas through photosynthesis, as well as high requirements for the hygiene and environmental protection of color regulators. Therefore, it is undoubtedly of great commercial value to invent a regulator that can not only regulate the color of azalea flowers, but also provide the nutrients needed to promote their growth, and has the characteristics of hygiene and environmental protection, thereby increasing the flowering life of azaleas.

[0007] The purpose of the present invention is to provide a method for achieving synthesis regulation of azalea cyanine by a chemical complexing agent sustained-release method, thereby achieving regulation of azalea flower color and obtaining multi-color bonsai azalea with a longer flower opening period. Summary of the invention

[0008] In order to solve the above technical problems and enhance the commercial value of potted azalea, the present invention proposes a color regulator for red flowers of azalea and a preparation and use method thereof.

[0009] The present invention is achieved through the following technical solutions:

[0010] A color regulator for red flowers of azalea, comprising an acidic buffer solution, wherein ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ Sustained release agent, sugar and Span-80 surfactant.

[0011] Further improvement, ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ The mass ratio of the sustained-release agent, the sugar and the Span-80 surfactant is 1.0: 0.50-0.70: 10.0-15.0: 0.10-0.15.

[0012] Further improvement, the Fe 2+ The sustained-release agent is one or any combination of salicylic acid, methyl salicylate and ethyl salicylate.

[0013] Further improvement, the ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· The final mass concentration of 6H2O] is 0.14% to 0.25%.

[0014] Further improvement, the Fe 2+ The final concentration of the sustained-release agent is 0.10% to 0.14%.

[0015] As a further improvement, the pH of the acidic buffer solution is 4.8-5.4, and the acidic buffer solution is potassium hydrogen phthalate-phosphate buffer solution, or citric acid-potassium phosphate buffer solution.

[0016] Further improvement, in terms of mass percentage, the final concentration of citric acid in the potassium hydrogen phthalate-phosphate buffer is 0.30% to 0.60%, and the final concentration of potassium hydrogen phthalate is 0.50% to 0.60%; the final concentration of citric acid in the citric acid-potassium phosphate buffer solution is 0.30% to 0.60%, and the final concentration of phosphate is 0.80% to 1.50%; the phosphate is any one of dipotassium hydrogen phosphate and potassium dihydrogen phosphate, or any combination of the two.

[0017] A method for preparing a rhododendron red flower color regulator comprises the following steps:

[0018] Step 1: first prepare a potassium hydrogen phthalate-phosphate buffer solution or a citric acid-potassium phosphate buffer solution with a pH of 4.8 to 5.4;

[0019] Step 2: Ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ The sustained-release agent, sugar and Span-80 surfactant are dissolved in an acidic buffer solution according to a mass ratio of 1.0: 0.50-0.70: 10.0-15.0: 0.10-0.15 to obtain a red flower color regulator for azalea, wherein ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· The final mass concentration of 6H2O] is 0.14% to 0.25%.

[0020] A method for using a rhododendron red flower color regulator, the rhododendron red flower color regulator as described above, comprising the following steps:

[0021] After the azalea buds begin to reveal some petals or open a few flowers, spray the azalea red flower color regulator within 30 cm around the roots of the azalea, spray once every 3 to 6 days, 20 to 80 mL each time, and spray continuously 2 to 6 times.

[0022] The method for using the azalea red flower color regulator according to claim 9, characterized in that the azalea is a bright red flower, a rose red flower, or an azalea with partially red flowers.

[0023] The regulation mechanism of the azalea red flower color regulator in the present invention is:

[0024] First, the higher the concentration of safflower cyanidin in azalea, the more red azalea. The safflower cyanidin with catechol structure derivative chelates with ferrous ions to form a stable five-membered chelate ring, which promotes the synthesis of safflower cyanidin, and iron ions precipitate in a weakly acidic solution system, can not form a complex with anthocyanidin, can not effectively promote the synthesis of anthocyanidin, can not make the maximum absorption wavelength of safflower cyanidin red shift, so, the red flowers of azalea need a certain concentration of ferrous ions to cultivate. The present invention uses ammonium ferrous sulfate hexahydrate to ensure the presence of a certain concentration of ferrous ions, and uses ammonium ferrous sulfate hexahydrate instead of ferrous sulfate to avoid ferrous ions being easily oxidized to iron ions, and then forms iron hydroxide precipitation and loses the effect of promoting biochemical synthesis of safflower cyanidin.

[0025] Second, ferrous ions can exist stably in weakly acidic solutions, which is conducive to promoting the synthesis of safflower cyanin, and phosphate ions can also complex with ferrous ions to further stabilize ferrous ions. Using citric acid or potassium hydrogen phthalate and potassium phosphate to prepare a buffer solution can not only maintain the weak acidity of the color regulator, but also prevent ferrous ions from being oxidized, and provide the potassium source required for the development and growth of azaleas.

[0026] Third, salicylic acid and its methyl salicylate and ethyl salicylate derivatives have the function of promoting the root growth of azalea. 2+ The "memory", Fe 2+ The release "valve" has the function of capturing and slowly releasing Fe 2+ The role of Fe can meet the synthesis needs of azalea anthocyanins at each stage, thereby controlling the synthesis rate and amount of azalea anthocyanins. 2+ The "memory", Fe 2+ The release "valve" is because the phenolic hydroxyl group in salicylic acid and its derivatives can complex with ferrous ions to form a relatively stable organic complex of ferrous ions, which plays a role in temporarily storing Fe 2+ The role of Fe 2+ Oxidized to Fe 3+ When anthocyanin is synthesized, Fe 2+ The simple complex formed with the hydroxyl groups on salicylic acid and its derivatives can release a certain amount of Fe 2+ Participate in biochemical synthesis reactions, releasing Fe 2+It forms a more stable ferrous ion five-membered chelate ring with catechol structural derivatives. In short, it is to use monophenol derivatives to form complexes with ferrous ions, and catechol derivatives to form more stable chelates with ferrous ions, and to achieve the transfer of ferrous ions between the two, thereby regulating the synthesis speed and amount of azalea anthocyanin, so that the flowers of the cultivated potted azaleas that bloom at different times have different color differences, showing the characteristics of flower color diversity.

[0027] Finally, salicylic acid and its derivatives can also promote the growth of the rhododendron plant's root system, further promoting the growth of the rhododendron plant's branches and leaves, absorbing more nutrients, and thus extending the average lifespan of the rhododendron flowers.

[0028] The beneficial effects of the azalea red flower color regulator of the present invention are:

[0029] 1) When the azalea buds just open and reveal red petals, the azalea color regulator is applied intermittently and repeatedly to make the color of the azalea flowers fade gradually, thereby showing the multi-color characteristics of the same pot of azalea flowers.

[0030] 2) Utilize Fe 2+ It forms a stable five-membered ring chelate with catechol structure derivatives to give stability to anthocyanin, and uses salicylic acid and its derivatives to promote the growth of azalea plants, making the flowering period of azalea flowers longer.

[0031] The experimental object of the azalea flower color enhancer of the present invention is the four-season azalea, which is a variety bred and cultivated by the company. This variety has the characteristics of blooming in three seasons a year, which is conducive to seeing the effect of gradual color changes of the flowers. According to the cultivation and color change mechanism of the azalea color regulator on azalea, it can be inferred that other varieties of azalea have similar effects and should be protected by corresponding patent rights. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1a This is a color picture of a bright red azalea flower (single flower) before spraying the reagent of the present invention in Example 1;

[0033] Figure 1b This is a color picture of a bright red azalea flower (single flower) after spraying the reagent of the present invention in Example 1;

[0034] Figure 1c This is a color picture of a rose-red azalea flower (single flower) before spraying the reagent of the present invention in Example 2;

[0035] Figure 1d This is a color picture of a rose-red azalea flower (single flower) after spraying the reagent of the present invention in Example 2;

[0036] Figure 2aThis is a color picture of a rose-red azalea flower (single flower) before spraying the reagent of the present invention in Example 4;

[0037] Figure 2b This is a color picture of a rose-red azalea flower (single flower) after spraying the reagent of the present invention in Example 4;

[0038] Figure 3a This is a color picture of the rose-red azalea bud before spraying the reagent of the present invention in Example 5;

[0039] Figure 3b This is a color picture of the rose-red azalea bud after spraying the reagent of the present invention in Example 5; DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0041] The preparation process of the azalea red flower color regulator of the present invention is as follows:

[0042] Weigh a certain amount of citric acid (C4H2O7·H2O) or potassium hydrogen phthalate, potassium phosphate, dissolve in water, stir to dissolve, add water to a certain volume, and prepare a buffer solution with a pH value of 4.8 to 5.4. The mass concentration of citric acid is 0.3% to 0.6%, the mass concentration of potassium hydrogen phthalate is 0.50% to 0.60%, and the mass concentration of potassium phosphate is 0.80% to 1.50%. The potassium phosphate is one of potassium dihydrogen phosphate and dipotassium hydrogen phosphate, or a combination of the two in a certain ratio.

[0043] Weigh ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ The sustained-release agent, sugar and Span-80 surfactant are dissolved in the above buffer solution, stirred to dissolve, and stored in a sealed container. The mass ratio of each component in the color control agent is ammonium ferrous sulfate hexahydrate: salicylic acid or salicylic acid derivative: sugar: Span-80 = 1.0: 0.50-0.70: 10-15: 0.10-0.15, wherein the concentration of ammonium ferrous sulfate hexahydrate is 0.14%-0.25%, and the concentration of Fe 2+ The concentration of the sustained-release agent is 0.10% to 0.14%.

[0044] The application of the red flower color regulator of azalea in the present invention is as follows: after the azalea buds begin to reveal a little petal or open a few flowers, the red flower color regulator of azalea is sprayed within 30 cm around the root of the azalea, sprayed once every 3 to 6 days, sprayed 2 to 6 times continuously, the amount of each spraying is 20 to 80 mL, depending on the volume of the azalea, and tap water is sprayed once every 1 day to keep the soil moist. For azalea seedlings, the concentration of the color regulator can also be diluted 2 to 3 times before use.

[0045] Example 1 to Example 2

[0046] Weigh 10.2g of citric acid (C4H2O7·H2O) and 26.5g of dipotassium hydrogen phosphate (K2HPO4·3H2O), dissolve them in water, and add water to make the volume 2000mL to obtain a citric acid and dipotassium hydrogen phosphate buffer solution with a pH of 4.8, wherein the concentration of citric acid is about 0.50% and the concentration of dipotassium hydrogen phosphate is about 1.30%.

[0047] Note: For substances with crystalline water, the molecular weight of crystalline water is included in the mass percentage concentration, the same below.

[0048] Weigh 2.70 g salicylic acid, 5.00 g ferrous ammonium sulfate [Fe(NH4)2·(SO4) 2· 6H2O], 50.00g of glucose and 0.50g of Span-80 emulsifier are dissolved in the aforementioned 2000mL buffer solution of pH=4.8 composed of citric acid and dipotassium hydrogen phosphate, and the solution is fully stirred and dissolved to obtain a red flower color regulator for azalea with a salicylic acid concentration of about 0.14% and an ammonium ferrous sulfate hexahydrate concentration of about 0.24%, which is then stored in a sealed container.

[0049] The potted four-season azalea with bright red flowers (Example 1) and rose red flowers (Example 2) was used for experiment. After the azalea was cultivated with tap water to open more than 3 flowers, the above-mentioned azalea color regulator solution was sprayed within 30 cm around the root of the four-season azalea seedlings, sprayed once every 4 days, 50 mL each time, and tap water was sprayed once every 1 day to keep the soil moist, and the experiment was carried out continuously for 30 days. As the azalea blooms later, the red color of the flower becomes lighter, so that the flowers on the same azalea show very obvious color difference, that is, a multi-color azalea is obtained. The color changes of the bright red azalea and the rose red azalea flowers (single flower) are shown in Figure 2. Figure 1a , Figure 1b and Figure 1c , Figure 1d .

[0050] From the time when the red petals of the azalea buds just appeared to the time when the flowers fell off, the number of days each flower was open was recorded, and the average number of days was taken as the life span of the flowers. The experimental results are shown in Table 1. At the same time, the 3nh NR110 handheld colorimeter was used to measure two flowers of the same azalea plant that were fully open in the early stage (7th day) cultivated with tap water and two flowers that were fully open in the middle stage (14th day, i.e., 7 days later) cultivated with color regulators. The chromaticity was measured at the close positions of the five petals of each flower, and the average red chromaticity (a) was calculated. The experimental results are shown in Table 1.

[0051] Comparative Example 1, Comparative Example 2

[0052] 2.70 g of benzoic acid was used instead of 2.70 g of salicylic acid. According to the experimental process of Example 1, two potted four-season azaleas, i.e., azaleas with bright red flowers (Comparative Example 1) and azaleas with rose red flowers (Comparative Example 2) cultivated in the same period, were cultivated, the flower opening conditions were recorded, and the petal chromaticity was measured. The experimental results are shown in Table 1.

[0053] Table 1 Effects of salicylic acid-containing color regulator and benzoic acid-containing solution on the flower life and color of two azalea species

[0054]

[0055] Compare Example 1 and Example 2 with Comparative Example 1 and Comparative Example 2. The experimental results show that: 1) The flowers cultivated with the color regulator containing salicylic acid have lighter red petals as the flowering period progresses. The red chromaticity of the petals of the two flowers in full bloom in the middle period is significantly lower than that of the flowers cultivated with water in the early stage, among which the average red chromaticity of the petals of the bright red flowers is reduced by about 28%, and the average red chromaticity of the petals of the rose red flowers is reduced by about 33%. 2) The two azalea flowers cultivated with the color regulator containing benzoic acid instead of salicylic acid have no obvious change in the red color of the petals at different flowering periods, and the red chromaticity is close, indicating that benzoic acid basically has no effect on regulating the red chromaticity of the azalea petals. 3) The flowering period of the bright red flowers and rose red flowers cultivated with the color regulator containing salicylic acid is significantly longer than that of the two azalea flowers cultivated with the color regulator containing benzoic acid and directly with water, and the flowering period life span increases by about 28%.

[0056] It can be seen that the azalea flower color regulator composed of salicylic acid-ammonium ferrous sulfate and citric acid-dipotassium hydrogen phosphate has a good effect of reducing the red color of azalea flowers, and can obtain a multi-color effect for the same potted azalea.

[0057] Embodiment 3-embodiment 4

[0058] Weigh 12.0 g potassium hydrogen phthalate and 30.0 g potassium dihydrogen phosphate (K2HPO4.3H2O), dissolve in water, and adjust the volume to 2000 mL to obtain a potassium hydrogen phthalate-potassium dihydrogen phosphate buffer solution with a pH of 5.4, wherein the concentration of potassium hydrogen phthalate is about 0.59% and the concentration of potassium dihydrogen phosphate is about 1.5%.

[0059] Weigh 2.10 g of ethyl salicylate, 3.00 g of ammonium ferrous sulfate [Fe(NH4)2·(SO4) 2· 6H2O], 45.0g of glucose and 0.40g of Span-80 emulsifier are dissolved in the aforementioned 2000mL buffer solution of pH=5.4 composed of potassium hydrogen phthalate and dipotassium hydrogen phosphate, and the solution is fully stirred and dissolved to obtain a red flower color regulator for azalea with an ethyl salicylate concentration of about 0.10% and an ammonium ferrous sulfate concentration of about 0.14%, which is then stored in a sealed container.

[0060] The experiment was conducted with potted four-season azaleas with bright red flowers (Example 3) and rose-red flowers (Example 4). After three or more flowers were grown with tap water (early stage), the above-mentioned azalea flower color regulator (mid-stage) was sprayed within 30 cm around the roots of the four-season azalea seedlings. The spraying was done once every four days, 80 mL each time, and tap water was sprayed once every day to keep the soil moist. This was continued for 30 days. It can be seen that the colors of the azalea flowers that opened at different times were obviously different. The colors of the rose-red azalea flowers (single flower) that opened in the early stage and the mid-stage were different. Figure 2a , Figure 2b .

[0061] From the time when the red petals of the azalea buds just appeared to the time when the flowers fell off, the number of days each flower was open was recorded, and the average number of days was taken as the life span of the flowers. At the same time, the 3nh NR110 handheld colorimeter was used to measure two flowers of the same azalea plant that were fully open in the early stage (6th day) cultivated with tap water and two flowers that were fully open in the middle stage (14th day, i.e., 8 days later). The chromaticity was measured at the close positions of the five petals of each flower, and the average red chromaticity (a) was calculated. The experimental results are shown in Table 2.

[0062] The results show that: 1) The red color of the petals of flowers cultivated with the color regulator containing ethyl salicylate becomes lighter as the flowering period progresses. The red chromaticity of the petals of the two flowers in full bloom in the middle period is also significantly lower than the average red chromaticity of the flowers cultivated with water in the early period. The red chromaticity of the petals of the bright red flowers is reduced by about 25%, and the red chromaticity of the petals of the rose red flowers is reduced by about 28%. 2) The color difference and flowering life of the bright red and rose red flowers cultivated by the flower color regulator composed of ethyl salicylate-ammonium ferrous sulfate-potassium hydrogen phthalate and dipotassium hydrogen phosphate are also similar to those of the flower color regulator composed of salicylic acid-ammonium ferrous sulfate-citric acid and dipotassium hydrogen phosphate, indicating that ethyl salicylate also has a similar effect to salicylic acid.

[0063] Example 5

[0064] Weigh 6.2g of citric acid (C4H2O7·H2O) and 16.5g of dipotassium hydrogen phosphate (K2HPO4·3H2O), dissolve them in water, and add water to make the volume 2000mL to obtain a citric acid and dipotassium hydrogen phosphate buffer solution with a pH of 4.8, wherein the concentration of citric acid is about 0.31% and the concentration of dipotassium hydrogen phosphate is about 0.81%.

[0065] Weigh 2.30 g salicylic acid, 4.00 g ferrous ammonium sulfate [Fe(NH4)2·(SO4) 2· 6H2O], 46.00g of glucose and 0.60g of Span-80 emulsifier are dissolved in the aforementioned 2000mL buffer solution of pH=4.8 composed of citric acid and dipotassium hydrogen phosphate, and the solution is fully stirred to obtain a red flower color regulator of azalea with a salicylic acid concentration of about 0.11% and an ammonium ferrous sulfate concentration of about 0.19%, which is then stored in a sealed container.

[0066] The experiment was conducted with potted four-season azaleas with rose-red flowers. When the flower buds just showed some red petals, the above flower color regulator solution was sprayed within 30cm around the roots of the four-season azalea seedlings. Spray once every 3 days, 40mL each time, for a total of 2 times, and spray tap water once every 1 day to keep the soil moist but not wet. After the 6th day, it can be seen that the light rose-red half-opened flower buds are visible. Figure 3a The 3nh NR110 handheld colorimeter was used to measure the color of two flower buds on the same azalea plant, and the chromaticity was measured at three positions of each flower bud, and the average red chromaticity (a) was calculated. The experimental results are shown in Table 2.

[0067] Comparative Example 3

[0068] The azalea red flower color regulator was prepared according to the process of Example 5, but without salicylic acid, and the potted rose red flower azalea cultivated by cuttings in the same period was used, and the flower cultivation experiment was carried out according to the process of Example 5 in the same period. After the 6th day, it can be seen that the rose red half-opened buds are Figure 3b The two flower buds on the same azalea were measured using a 3nh NR110 handheld colorimeter, and the red chromaticity values ​​were measured at three locations on each flower bud, and the average red chromaticity (a) was calculated. The experimental results are shown in Table 2.

[0069] Comparing Example 5 with Comparative Example 3, the flower bud color of the potted rose red flower azalea cultivated by the color regulator of Example 5 is obviously brighter than the flower bud color of the potted rose red flower azalea cultivated by the color regulator of Comparative Example 3 without adding salicylic acid ( Figure 3b ) is lighter, and its red chromaticity value is about 35% lower. It can be seen that salicylic acid plays a role in regulating the content of pigment glycosides during the opening process of Rhododendron sempervirens.

[0070] Table 2 Effects of salicylic acid-containing color regulators and other solutions on the life span and color of three types of azalea flowers

[0071]

[0072] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all details in detail, nor do they limit the invention to only the specific implementation methods described.

Claims

1. A color regulator for red flowers of azalea, characterized in that: The acidic buffer solution comprises ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ Sustained release agent, sugar and Span-80 surfactant.

2. The azalea red flower color regulator according to claim 1, characterized in that: The ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ The mass ratio of the sustained-release agent, the sugar and the Span-80 surfactant is 1.0: 0.50-0.70: 10.0-15.0: 0.10-0.

15.

3. The azalea red flower color regulator according to claim 1, characterized in that: The Fe 2+ The sustained-release agent is one or any combination of salicylic acid, methyl salicylate and ethyl salicylate.

4. The azalea red flower color regulator according to claim 1, characterized in that: The ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· The final mass concentration of 6H2O] is 0.14% to 0.25%.

5. The azalea red flower color regulator according to claim 1, characterized in that: The Fe 2+ The final concentration of the sustained-release agent is 0.10% to 0.14%.

6. The azalea red flower color regulator according to claim 1, characterized in that: The pH of the acidic buffer solution is 4.8-5.4, and the acidic buffer solution is potassium hydrogen phthalate-phosphate buffer solution or citric acid-potassium phosphate buffer solution.

7. The azalea red flower color regulator according to claim 6, characterized in that: In terms of mass percentage, the final concentration of citric acid in the potassium hydrogen phthalate-phosphate buffer is 0.30% to 0.60%, and the final concentration of potassium hydrogen phthalate is 0.50% to 0.60%; The final concentration of citric acid in the citric acid-potassium phosphate buffer solution is 0.30% to 0.60%, and the final concentration of phosphate is 0.80% to 1.50%; the phosphate is any one of dipotassium hydrogen phosphate and potassium dihydrogen phosphate or any combination of the two.

8. A method for preparing a rhododendron red flower color regulator, characterized in that: The steps include: Step 1: first prepare a potassium hydrogen phthalate-phosphate buffer solution or a citric acid-potassium phosphate buffer solution with a pH of 4.8 to 5.4; Step 2: Ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], Fe 2+ The sustained-release agent, sugar and Span-80 surfactant are dissolved in an acidic buffer solution according to a mass ratio of 1.0: 0.50-0.70: 10.0-15.0: 0.10-0.15 to obtain a red flower color regulator for azalea, wherein ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· The final mass concentration of 6H2O] is 0.14% to 0.25%.

9. A method for using a rhododendron red flower color regulator, characterized in that: The azalea red flower color regulator is as described in any one of claims 1 to 7, comprising the following steps: After the azalea buds begin to reveal some petals or open a few flowers, spray the azalea red flower color regulator within 30 cm around the roots of the azalea, spray once every 3 to 6 days, 20 to 80 mL each time, and spray continuously 2 to 6 times.

10. The method for using the azalea red flower color regulator according to claim 9, characterized in that: The azalea flowers include bright red flowers, rose red flowers and azalea flowers with red flowers in some parts.

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