Tissue cell extraction and collection device

Abstract

The invention discloses a tissue cell extracting and collecting device, and relates to the technical field of cell extraction.The tissue cell extracting and collecting device comprises a base and a rotating seat, the rotating seat is fixedly installed in the middle of the base, the top of the rotating seat is rotationally connected with a rotating disc, and an oscillation assembly is arranged in the rotating seat; through the arrangement of the oscillation assembly, the oscillation effect on a fixed test tube in the test tube rack is realized, so that the fusion of trypsin and a tissue sample is accelerated, the test tube filled with tissue sample cell sap does not need to be moved, the pollution risk is reduced, and meanwhile, the risk of breaking the fragile test tube is also reduced; through cooperation of the electric telescopic rod and the lantern ring, the swing amplitude of the swing shaft is larger than that during oscillation, so that tissue cells are promoted to be separated from cell sap, a test tube containing tissue sample cell sap does not need to be moved, the pollution risk is further reduced, and meanwhile the breaking risk of the fragile test tube is further reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell extraction, in particular to a tissue cell extraction and collection device. Background Art

[0002] When diagnosing a disease and performing medical treatment on a patient, it is necessary to collect tissue cells from the patient's affected area in order to perform subsequent diagnosis and treatment based on the patient's tissue cells.

[0003] After collecting tissue samples from patients, they are usually broken, and then enzyme solutions are added and assisted by oscillation, centrifugation and other operations to collect separated tissue cells. In this process, scissors or scalpels are needed to break the tissue, and then a filter is needed to remove excess tissue fragments and impurities. Different test tubes are needed for filtration, which increases the possibility of tissue cell contamination. After adding the enzyme solution, the test tubes are moved between different instruments during oscillation and centrifugation, further increasing the risk of contamination of the tissue cell solution. It also increases the risk of accidental rupture of the test tube, which has a certain impact on the reliability and safety of the sample. After centrifugation, a pipette is needed to separate the tissue cells from the liquid. The pipette is directly inserted into the liquid and extracted, so that the tissue cells can easily enter the pipette driven by the cell fluid, thereby reducing the number of tissue cell samples during the pipetting process, which interferes with the subsequent analysis and use of the tissue cells.

[0004] In order to solve the above problems, the inventor proposed a tissue cell extraction and collection device. Summary of the invention

[0005] In order to solve the above technical problems, a tissue cell extraction and collection device is provided.

[0006] To achieve the above objectives, the present invention can be implemented by the following technical solutions:

[0007] The present invention provides a tissue cell extraction and collection device, comprising: a base and a rotating base, wherein the rotating base is fixedly installed in the middle of the base, a rotating disk is rotatably connected to the top of the rotating base, and an oscillating component is arranged inside the rotating base;

[0008] The oscillation assembly includes a swing shaft rotatably connected in a rotating seat, an oscillation plate is rotatably connected to the middle part of the turntable, the top of the swing shaft is fixedly connected to the oscillation plate, a double-axis motor is fixedly installed in the rotating seat, a fixed seat 1 is fixedly installed in the rotating seat, the fixed seat 1 is located between the swing shaft and the double-axis motor, a side of the fixed seat 1 away from the double-axis motor is rotatably connected to the rotating shaft 1, an output shaft of the double-axis motor close to the swing shaft passes through the fixed seat 1, the output shaft of the double-axis motor passing through the fixed seat 1 is fixedly connected to the rotating shaft 1, a collar is sleeved on the surface of the rotating shaft 1, a sleeve is slidably connected to the rotating shaft 1, one end of the sleeve is rotatably connected to the collar Dynamic connection, a fixed shaft 1 is fixedly installed on the top of the sleeve, a pin is inserted on the swing shaft, and both ends of the pin are rotatably connected with a pin sleeve, a side of the pin sleeve close to the dual-axis motor is fixedly connected with a rotating shaft 2, and a side of the rotating shaft 2 away from the pin sleeve is fixedly connected with two fixed plates, the two fixed plates are symmetrically arranged on the rotating shaft 2, and a fixed shaft 2 is commonly inserted on the two fixed plates, an arc plate is arranged between the two fixed plates, an arc groove is opened on the arc plate, and the fixed shaft 2 is slidably connected in the arc groove, a connecting block is fixedly connected to the side of the arc plate close to the dual-axis motor, and an end of the connecting block away from the arc plate is rotatably connected with a connecting arm.

[0009] Preferably, there are no less than two connecting arms, and the two connecting arms are symmetrically arranged on the connecting block. The connecting arm is L-shaped, and the bending part of the connecting arm is rotatably connected to the connecting block.

[0010] Preferably, a slide groove 1 is provided on one end of the connecting arm close to the fixed plate, and the two ends of the fixed shaft 2 are inserted in the slide groove 1, and the fixed shaft 2 forms a limit fit with the connecting arm through the slide groove 1; a slide groove 2 is provided on one end of the connecting arm close to the dual-axis motor, and the two ends of the fixed shaft 1 are inserted in the slide groove 2, and the fixed shaft 1 forms a limit fit with the connecting arm through the slide groove 2.

[0011] Preferably, the side of the sleeve is fixedly connected to a limiting block, a mounting seat is fixedly connected to one side of the fixing seat close to the limiting block, an electric telescopic rod is fixedly installed on one side of the mounting seat close to the limiting block, and a movable axis of the electric telescopic rod is fixedly connected to the limiting block.

[0012] Preferably, the top of the oscillating plate is detachably connected to a test tube rack.

[0013] Preferably, a filter lifting assembly is provided on the base, and the filter lifting assembly includes a gear fixedly connected to the output shaft of one side of the dual-axis motor away from the fixed base, the bottom of the turntable is fixedly connected to a bevel gear, the gear is meshed with the bevel gear, the top of the base is fixedly connected to a fixing frame, the side of the fixing frame close to the rotating seat is fixedly connected to a second fixing seat, the bottom of the second fixing seat is rotatably connected to a nut, the internal thread of the nut is connected to a lead screw, the top of the lead screw passes through the second fixing seat, the top of the turntable is fixedly connected to a connecting frame, there are no less than two connecting frames, two of the connecting frames are symmetrically arranged on the turntable, the top ends of the two connecting frames are respectively fixedly connected to the two sides of the nut, and the bottom end of the lead screw is fixedly connected to a connecting rod.

[0014] Preferably, the length of the lead screw is the same as the distance between the second fixing seat and the test tube rack.

[0015] Preferably, a filter assembly is provided at the bottom of the connecting rod, and the filter assembly includes a fixing ring fixedly connected to the connecting rod, a cell filter is sleeved on the bottom of the connecting rod, a connecting seat is fixedly connected to the top of the cell filter, there are no less than two connecting seats, and two connecting seats are symmetrically arranged on the cell filter, and fixing rods are fixedly connected on both sides of the fixing ring, and a sliding groove is provided on the side of the two fixing rods away from the fixing ring, and a plug rod is slidably connected in the two sliding grooves, and springs are provided in the two sliding grooves, and slots are provided on the side of the two connecting seats close to the fixing ring, and the two plug rods are respectively snap-fitted with the two slots, and the two plug rods are respectively sleeved with a shift block.

[0016] Preferably, the middle portion of the cell filter has a socket, and the bottom of the connecting rod forms a sliding fit with the cell filter through the socket.

[0017] Preferably, a sealing gasket is provided at the connection between the slide groove and the insertion rod.

[0018] As described above, the characteristics and advantages of a tissue cell extraction and collection device in the present invention are:

[0019] Through the setting of the oscillation component, when the dual-axis motor drives the rotating shaft 1 to rotate continuously, the arc plate drives the swing shaft to rotate back and forth through the fixed plate, the rotating shaft 2 and the pin sleeve, so as to drive the oscillation plate on the top of the swing shaft to rotate back and forth, thereby achieving an oscillation effect on the test tube fixed in the test tube rack, so as to accelerate the fusion of trypsin and tissue samples, thereby accelerating the separation speed of tissue cells from the tissue. Compared with the existing setting using an oscillator, there is no need to move the test tube containing the cell fluid of the tissue sample, which reduces the risk of contamination and also reduces the risk of fragile test tubes breaking;

[0020] Through the cooperation of the electric telescopic rod and the collar, after the electric telescopic rod is extended to push the limit block to move, the connection between the connection block and the connection arm gradually moves away from the rotating shaft 1. As the inclination angle of the arc plate increases, the swing shaft rotates toward the connecting arm, which is farther away at this time, under the action of the pin sleeve and the rotating shaft 2 during the rotation of the arc plate, so that the swing amplitude of the swing shaft is larger than that during oscillation, so as to promote the separation of tissue cells and cell fluid. Compared with centrifugation using a centrifuge, there is no need to move the test tube containing the tissue sample cell fluid, which further reduces the risk of contamination and further reduces the risk of fragile test tube breakage, effectively avoiding operations that may cause sample failure during tissue cell extraction and collection;

[0021] Through the setting of the filter lifting component, after the broken tissue cell sample liquid is poured into the test tube, the tissue cell sample liquid falls into the inside of the cell filter. Under the filtering effect of the cell filter, the tissue cells to be extracted fall into the inside of the test tube through the filter holes on the cell filter, and the excess tissue samples remain in the cell filter, so as to achieve the effect of separating the excess tissue samples to be discarded. Then, the output shaft fixedly connected to the oscillating disk and the gear rotates in the opposite direction, so that the nut drives the lead screw to rise, and the separated excess tissue samples are taken out of the test tube. At this time, the filter inside the cell filter and the test tube can be filtered, which reduces the risk of contamination of the tissue cell sample during the transfer process, so as to improve the reliability of the tissue cell sample.

[0022] By setting the filter component, the cell filter can be quickly replaced. After the replacement is completed, the end of the dual-axis motor fixedly connected to the gear is started, and the screw pushes the cell filter into the test tube again. As the cell filter gradually descends, the liquid in the test tube enters the cell filter through the filter holes, while the cells cannot pass through the cell filter and are pushed to the bottom of the test tube. When the cells are separated from the cell fluid, a pipette is used to extract the separated cell fluid, thereby separating the cells from the cell fluid. Compared with the direct pipetting operation in the prior art, this avoids the situation where some cells are brought into the pipette by the cell fluid, thereby ensuring the number of collected tissue cell samples and avoiding the situation where the amount of tissue cell samples collected is not accurate enough. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a three-dimensional schematic diagram of the overall structure shown in the present invention;

[0024] Figure 2 It is a three-dimensional schematic diagram of the overall structure shown in the present invention;

[0025] Figure 3 It is a three-dimensional schematic diagram of the overall structure shown in the present invention;

[0026] Figure 4 It is a three-dimensional schematic diagram of the internal structure of the rotating seat shown in the present invention;

[0027] Figure 5 It is a three-dimensional schematic diagram of the overall structure of the oscillation component shown in the present invention;

[0028] Figure 6 It is a three-dimensional schematic diagram of the overall structure of the oscillation component shown in the present invention;

[0029] Figure 7 The present invention shows Figure 6 Enlarged view of point A in the middle;

[0030] Figure 8 It is a top perspective schematic diagram of the overall structure of the oscillation assembly shown in the present invention;

[0031] Fig. 9 It is a three-dimensional schematic diagram of the cell filter structure shown in the present invention;

[0032] Fig.10 It is a cross-sectional stereoscopic schematic diagram of the cell filter structure shown in the present invention.

[0033] The reference numerals in the drawings of the present invention are: 1, base; 2, rotating seat; 3, turntable;

[0034] Oscillation assembly: 401, swing shaft; 402, oscillation plate; 403, dual-axis motor; 404, fixed seat 1; 405, rotating shaft 1; 406, collar; 407, sleeve; 408, fixed shaft 1; 409, latch; 410, pin sleeve; 411, rotating shaft 2; 412, fixed plate; 413, fixed shaft 2; 414, arc plate; 415, arc groove; 416, connecting block; 417, connecting arm; 418, slide 1; 419, slide 2; 420, limit block; 421, mounting seat; 422, electric telescopic rod; 423, test tube rack;

[0035] Filter lifting assembly: 501, gear; 502, bevel gear; 503, fixing frame; 504, fixing seat 2; 505, nut; 506, lead screw; 507, connecting frame; 508, connecting rod;

[0036] Filter assembly: 601, fixing ring; 602, cell filter; 603, connecting seat; 604, fixing rod; 605, slide groove; 606, plug rod; 607, spring; 608, slot; 609, dial block. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0038] See 1~ Fig.10 As shown, it is an embodiment provided by the present invention, and a tissue cell extraction and collection device provided will be described in detail below:

[0039] A tissue cell extraction and collection device, such as Figures 1 to 8 As shown, it comprises: a base 1 and a rotating base 2, the rotating base 2 is fixedly mounted in the middle of the base 1, a turntable 3 is rotatably connected to the top of the rotating base 2, and an oscillating component is arranged inside the rotating base 2;

[0040] The oscillation assembly includes a swing shaft 401 rotatably connected to the rotating seat 2, an oscillation disk 402 is rotatably connected to the middle of the turntable 3, the top of the swing shaft 401 is fixedly connected to the oscillation disk 402, a dual-axis motor 403 is fixedly installed in the rotating seat 2, a fixed seat 1 404 is fixedly installed in the rotating seat 2, the fixed seat 1 404 is located between the swing shaft 401 and the dual-axis motor 403, a side of the fixed seat 1 404 away from the dual-axis motor 403 is rotatably connected to a rotating shaft 1 405, an output shaft of the dual-axis motor 403 close to the swing shaft 401 side passes through the fixed seat 1 404, the output shaft of the dual-axis motor 403 passing through the fixed seat 1 404 is fixedly connected to the rotating shaft 1 405, a collar 406 is sleeved on the surface of the rotating shaft 1 405, and the rotating shaft 1 40 5 is slidably connected with a sleeve 407, a collar 406 is used to push the sleeve 407 to slide on the rotating shaft 1 405, one end of the sleeve 407 is rotatably connected with the collar 406, a fixed shaft 1 408 is fixedly installed on the top of the sleeve 407, and the fixed shaft 1 408 is located on the side of the sleeve 407 close to the fixed seat 1 404, a latch 409 is inserted on the swing shaft 401, and both ends of the latch 409 are rotatably connected with a pin sleeve 410, a side of the pin sleeve 410 close to the dual-axis motor 403 is fixedly connected with a rotating shaft 2 411, and a side of the rotating shaft 2 411 away from the pin sleeve 410 is fixedly connected with two fixed plates 412, the two fixed plates 412 are symmetrically arranged on the rotating shaft 2 411, and the two fixed plates 412 are commonly inserted with a fixed shaft 2 411 13, an arc plate 414 is arranged between the two fixed plates 412, an arc groove 415 is provided on the arc plate 414, the fixed shaft 2 413 is slidably connected in the arc groove 415, a connecting block 416 is fixedly connected to the side of the arc plate 414 close to the dual-axis motor 403, and a connecting arm 417 is rotatably connected to the end of the connecting block 416 away from the arc plate 414, and there are no less than two connecting arms 417, and the two connecting arms 417 are symmetrically arranged on the connecting block 416, and the connecting arms 417 are L-shaped, and the bending part of the connecting arms 417 is rotatably connected to the connecting block 416, and a sliding groove 418 is provided on the end of the connecting arm 417 close to the fixed plate 412, and both ends of the fixed shaft 2 413 are inserted in the sliding groove 418, and the fixed shaft 2 413 is slidably connected to the fixed shaft 2 The groove 1 418 forms a limit fit with the connecting arm 417, and a slide groove 2 419 is provided on one end of the connecting arm 417 close to the dual-axis motor 403. Both ends of the fixed shaft 1 408 are inserted into the slide groove 2 419, and the fixed shaft 1 408 forms a limit fit with the connecting arm 417 through the slide groove 2 419. The side of the collar 406 is fixedly connected to the limit block 420, and the side of the fixed seat 1 404 close to the limit block 420 is fixedly connected with the mounting seat 421, and the side of the mounting seat 421 close to the limit block 420 is fixedly installed with an electric telescopic rod 422, and the movable shaft of the electric telescopic rod 422 is fixedly connected to the limit block 420. The top of the oscillating disk 402 is detachably connected with a test tube rack 423, and the test tube rack 423 is mounted on the oscillating disk 402 by bolts.Different test tube racks 423 can be replaced according to the specifications of the test tubes;

[0041] Further, such as Figure 1 to Figure 4 As shown, a filter lifting assembly is provided on the base 1, and the filter lifting assembly includes a gear 501 fixedly connected to the output shaft of the dual-axis motor 403 away from the fixed seat 1 404 side, a bevel gear 502 is fixedly connected to the bottom of the turntable 3, and the gear 501 is meshed with the bevel gear 502. The top of the base 1 is fixedly connected to a fixing frame 503, and the side of the fixing frame 503 close to the rotating seat 2 is fixedly connected to a fixing seat 2 504, and the fixing seat 2 504 is located directly above the test tube rack 423, and the bottom of the fixing seat 2 504 A nut 505 is rotatably connected, and a lead screw 506 is connected to the inner thread of the nut 505. The top of the lead screw 506 passes through the second fixing seat 504. A connecting frame 507 is fixedly connected to the top of the turntable 3. There are no less than two connecting frames 507. The two connecting frames 507 are symmetrically arranged on the turntable 3. The top ends of the two connecting frames 507 are respectively fixedly connected to the two sides of the nut 505. The bottom end of the lead screw 506 is fixedly connected to a connecting rod 508. The length of the lead screw 506 is the same as the spacing between the second fixing seat 504 and the test tube rack 423.

[0042] Further, such as Fig. 9 , Fig.10 As shown, a filter assembly is provided at the bottom of the connecting rod 508, and the filter assembly includes a fixing ring 601 fixedly connected to the connecting rod 508, a cell filter 602 is sleeved at the bottom of the connecting rod 508, a connecting seat 603 is fixedly connected to the top of the cell filter 602, and there are no less than two connecting seats 603, and the two connecting seats 603 are symmetrically arranged on the cell filter 602, and fixing rods 604 are fixedly connected to the two sides of the fixing ring 601, and a sliding groove 605 is provided on the side of the two fixing rods 604 away from the fixing ring 601, and a plug rod 606 is slidably connected in the two sliding grooves 605, and the two A spring 607 is arranged in the slide groove 605, and the two ends of the spring 607 are fixedly connected to the slide groove 605 and the plug rod 606 respectively. A slot 608 is provided on the side of the two connecting seats 603 close to the fixed ring 601. The two plug rods 606 are respectively snap-fitted with the two slots 608. The two plug rods 606 are respectively sleeved with a shift block 609. The middle part of the cell filter 602 has a socket, and the bottom of the connecting rod 508 forms a sliding fit with the cell filter 602 through the socket. A sealing gasket is arranged at the connection between the slide groove 605 and the plug rod 606, which is used to prevent cell fluid from penetrating into the slide groove 605.

[0043] In combination with the above preferred embodiments, the following is the entire working process and working principle of the above embodiments:

[0044] The initial state is:

[0045] The dual-axis motor 403 is not started, the electric telescopic rod 422 is not extended, the sleeve 407 is located on the side of the rotating shaft 405 close to the fixed seat 404, the connecting arm 417 is not pushed by the fixed shaft 408, the fixed shaft 413 is located near the middle of the arc groove 415, so that the arc plate 414 is in an inclined state, the screw 506 does not move downward, the spring 607 is extended, so that the insertion rod 606 is located outside the slide groove 605, and the insertion rod 606 is stuck in the connecting seat 603, so that the cell filter 602 is fixed at the bottom of the connecting rod 508. This cell filter 602 is used to filter excess tissue samples after crushing. The cell filter 602 is close to the fixed seat 2 504.

[0046] The working status is:

[0047] Add tissue cell fluid:

[0048] When the tissue sample taken from the patient's body is crushed and the tissue cells need to be separated by enzyme digestion method later, the output shaft of the dual-axis motor 403 fixedly connected to the gear 501 is started, so that the gear 501 drives the turntable 3 to rotate through the meshing connection with the bevel gear 502. When the turntable 3 rotates, it drives the two connecting frames 507 to rotate, thereby driving the nut 505 to rotate, so that the lead screw 506 moves downward under the threaded connection with the nut 505, thereby driving the cell filter 602 at the bottom of the connecting rod 508 to enter the test tube fixed on the test tube rack 423. At this time, the crushed tissue cell sample liquid is poured into the test tube, and the tissue cell sample liquid falls on the cell filter After entering the test tube, under the filtering effect of the cell filter 602, the tissue cells to be extracted fall into the test tube through the filter holes on the cell filter 602, and the excess tissue samples remain in the cell filter 602, so as to achieve the effect of separating the excess tissue samples to be discarded. Then, the output shaft fixedly connected to the oscillating disk 402 and the gear 501 rotates in the opposite direction, so that the nut 505 drives the lead screw 506 to rise, and the separated excess tissue samples are taken out of the test tube. At this time, the filter inside the cell filter 602 and the test tube can be filtered, which reduces the risk of contamination of the tissue cell samples during the transfer process, so as to improve the reliability of the tissue cell samples.

[0049] Assisted cell separation:

[0050] After the tissue sample cell fluid is poured into the test tube, the end of the dual-axis motor 403 fixedly connected to the rotating shaft 1 405 is started, so that the dual-axis motor 403 drives the rotating shaft 1 405 to rotate, so that the rotating shaft 1 405 drives the sleeve 407 to rotate, thereby driving the fixed shaft 1 408 to rotate, and then the fixed shaft 1 408 drives the two connecting arms 417 to rotate with the rotating shaft 1 405 as the center of the circle through the limited cooperation with the second slide groove 419. At this time, the two connecting arms 417 drive the arc plate 414 to rotate through the rotation connection with the connecting block 416. At the same time, the connecting arm 417 cooperates with the fixed shaft 2 413 through the slide groove 1 418. In this state, due to the cooperation between the rotating shaft 2 411, the fixed shaft 2 413, and the arc plate 414, The plate 414 and the connecting block 416 rotate in cooperation with the connecting arm 417 and the fixed shaft 1 408, and the connecting arm 417 makes a circular motion with the rotating shaft 1 405 as the center, driving the fixed shaft 2 413 located in the arc groove 415 to rotate synchronously with the arc plate 414, thereby driving the rotating shaft 2 411 to rotate, so that when the arc plate 414 rotates to a non-vertical downward state driven by the connecting arm 417, the arc plate 414 in an inclined state with the rotating shaft 2 411 drives the rotating shaft 2 411 to deviate in the direction of the connecting arm 417, and drives the pin sleeve 410 to deviate in the direction of the connecting arm 417, so that the pin sleeve 410 drives the swing shaft 401 to rotate through the latch 409. When the sleeve 407 rotates in the rotating shaft 411, the rotating shaft 411 is rotated to the rotating shaft 411. After rotating ninety degrees under the action of the moving shaft 405, the fixed shaft 408 drives the connecting arm 417, the connecting block 416 and the arc plate 414 to gradually rotate to a vertical downward angle. In the process of the arc plate 414 gradually returning to the vertical downward position, the fixed plate 412, the rotating shaft 411 and the pin sleeve 410 drive the swing shaft 401 to return to its original position. Then, after the dual-axis motor 403 drives the rotating shaft 405 to rotate more than one hundred and eighty degrees, the arc plate 414 tilts again under the cooperation of the fixed shaft 408 and the connecting block 416, thereby driving the swing shaft 401 to rotate to the opposite side of the previous side through the fixed plate 412, the rotating shaft 411 and the pin sleeve 410. When the rotating shaft 405 rotates more than two hundred and seventy degrees After that, the swing shaft 401 is reset again when the arc plate 414 is reset to the vertical position, so that when the dual-axis motor 403 drives the rotating shaft 1 405 to rotate continuously, the arc plate 414 drives the swing shaft 401 to rotate back and forth through the fixed plate 412, the rotating shaft 2 411 and the pin sleeve 410, so as to drive the oscillation disk 402 on the top of the swing shaft 401 to rotate back and forth, thereby realizing the oscillation effect on the fixed test tube in the test tube rack 423, so as to accelerate the fusion of trypsin and tissue sample, thereby accelerating the separation speed of tissue cells from the tissue. Compared with the existing setting using an oscillator, there is no need to move the test tube containing the tissue sample cell fluid, which reduces the risk of contamination and the risk of fragile test tube breakage.

[0051] Centrifuge the tissue cell fluid:

[0052] After using the enzyme protein solution to separate the tissue cells, the tissue cell suspension needs to be centrifuged again to separate the tissue cells to be collected from the cell fluid. At this time, the electric telescopic rod 422 is started, so that the electric telescopic rod 422 pushes the limit block 420, thereby pushing the ring 406 to move away from the fixed seat 404, and then pushing the sleeve 407 to slide on the rotating shaft 405 toward the end away from the fixed seat 404, driving the fixed shaft 408 to approach the connecting arm 417, so that the fixed shaft 408 cooperates with the slide groove 419 to push the connecting arm The end of the connecting arm 417 with the second slide groove 419 makes the connecting arm 417 rotate in the direction of the fixed shaft 1 408 with the connection point with the connecting block 416 as the center, thereby driving the end of the connecting arm 417 with the slide groove 1 418 to rotate. Since the slide groove 1 418 is fixed on the fixed plate 412, the end of the connecting arm 417 with the slide groove 1 418 cannot rotate. Therefore, when the electric telescopic rod 422 is extended to make the fixed shaft 1 408 and the fixed shaft 2 413 approach each other, the connection between the connecting arm 417 and the connecting block 416 is due to the connection between the fixed shaft 1 408 and the fixed shaft 413. The positional relationship between the two shafts 413 changes and the connecting block 416 moves upward. When the connecting block 416 moves upward driven by the connecting arm 417, the arc plate 414 is constrained by the arc groove 415 and the fixed shaft 413 and cannot move upward. Therefore, when the connecting arm 417 drives the connecting block 416 to move upward, the inclination angle of the arc plate 414 increases under the action of the fixed shaft 413. At this time, the connection between the connecting block 416 and the connecting arm 417 gradually moves away from the rotating shaft 1 405. At this time, when the dual-axis motor 403 drives the rotating shaft 1 405 to rotate, due to the arc plate 414 The inclination angle increases, and during the rotation of the arc plate 414, the swing shaft 401 rotates toward the connecting arm 417, which is now farther away, under the action of the pin sleeve 410 and the rotating shaft 2 411, so that the swing amplitude of the swing shaft 401 is larger than that during oscillation, so as to promote the separation of tissue cells and cell fluid. Compared with centrifugation using a centrifuge, there is no need to move the test tube containing the tissue sample cell fluid, which further reduces the risk of contamination and the risk of fragile test tube breakage, effectively avoiding operations that may cause sample failure during tissue cell extraction and collection.

[0053] Assisted extraction of cell fluid:

[0054] After the cell sample to be collected is separated from the cell fluid, the two plug rods 606 are driven by hand through the two shift blocks 609 to slide out of the slots 608, so that the cell filter 602 loses its fixation, so that the cell filter 602 that has previously filtered out excess cell tissue is removed, and the cell filter 602 that can filter out the cell sample is replaced, and the middle part of the cell filter 602 is aligned with the connecting rod 508 and slides upward, so that the connecting seat 603 squeezes the plug rod 606 into the slide groove 605 and squeezes the spring 607, and then the plug rod 606 is aligned with the slot 608, and the plug rod 606 pops out under the action of the extension and reset of the spring 607, and the plug rod 606 extends into the slot 608 to jam the connecting seat 603, and the cell filter 602 is fixed, thereby realizing the quick replacement of the cell filter 602, and after the replacement is completed, the double The shaft motor 403 is fixedly connected to one end of the gear 501, so that the gear 501 drives the turntable 3, the connecting frame 507 and the nut 505 to rotate through the bevel gear 502, so that the screw 506 pushes the cell filter 602 into the test tube again. As the cell filter 602 gradually descends, the liquid in the test tube enters the top of the cell filter 602 through the filter holes, while the cells cannot pass through the cell filter 602 and are pushed to the bottom of the test tube. When the cells are separated from the cell fluid, a pipette is used to extract the separated cell fluid, thereby achieving separation of the cells and the cell fluid. Compared with the direct pipetting operation in the prior art, this avoids the situation where some cells are brought into the pipette by the cell fluid, thereby ensuring the number of collected tissue cell samples and avoiding the situation where the amount of tissue cell samples collected is not accurate enough.

[0055] The above descriptions are merely embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A tissue cell extraction and collection device, characterized in that: include: A base (1) and a rotating base (2), wherein the rotating base (2) is fixedly mounted in the middle of the base (1), a rotating disk (3) is rotatably connected to the top of the rotating base (2), and an oscillating component is arranged inside the rotating base (2); The oscillation assembly comprises an oscillating shaft (401) rotatably connected to a rotating seat (2); the middle part of the rotating plate (3) is rotatably connected to an oscillating plate (402); the top of the oscillating shaft (401) is fixedly connected to the oscillating plate (402); a dual-axis motor (403) is fixedly installed in the rotating seat (2); a fixed seat 1 (404) is fixedly installed in the rotating seat (2); the fixed seat 1 (404) is located between the oscillating shaft (401) and the dual-axis motor (403); the fixed seat 1 (404) is away from the dual-axis motor (403). One side of the motor (403) is rotatably connected to a rotating shaft (405); the output shaft of the dual-axis motor (403) on the side close to the swing shaft (401) passes through a fixed seat (404); the output shaft of the dual-axis motor (403) passes through the fixed seat (404) and is fixedly connected to the rotating shaft (405); a sleeve (406) is sleeved on the surface of the rotating shaft (405); a sleeve (407) is slidably connected to the rotating shaft (405); one end of the sleeve (407) is rotatably connected to the sleeve (406) A fixed shaft 1 (408) is fixedly installed on the top of the sleeve (407), a latch (409) is inserted on the swing shaft (401), and both ends of the latch (409) are connected to a pin sleeve (410) for common rotation, and a side of the pin sleeve (410) close to the dual-axis motor (403) is fixedly connected to a rotating shaft 2 (411), and a side of the rotating shaft 2 (411) away from the pin sleeve (410) is fixedly connected to two fixed plates (412), and the two fixed plates (412) are symmetrically arranged on the rotating shaft 2 (411). A second fixed shaft (413) is inserted into the two fixed plates (412) together, an arc plate (414) is arranged between the two fixed plates (412), an arc groove (415) is opened on the arc plate (414), the second fixed shaft (413) is slidably connected in the arc groove (415), a connecting block (416) is fixedly connected to one side of the arc plate (414) close to the dual-axis motor (403), and a connecting arm (417) is rotatably connected to one end of the connecting block (416) away from the arc plate (414).

2. A tissue cell extraction and collection device according to claim 1, characterized in that: No less than two connecting arms (417) are provided, and the two connecting arms (417) are symmetrically arranged on the connecting block (416). The connecting arm (417) is L-shaped, and the bending part of the connecting arm (417) is rotatably connected to the connecting block (416).

3. A tissue cell extraction and collection device according to claim 2, characterized in that: A slide groove 1 (418) is provided on one end of the connecting arm (417) close to the fixed plate (412), and both ends of the fixed shaft 2 (413) are inserted into the slide groove 1 (418). The fixed shaft 2 (413) forms a limit fit with the connecting arm (417) through the slide groove 1 (418). A slide groove 2 (419) is provided on one end of the connecting arm (417) close to the dual-axis motor (403), and both ends of the fixed shaft 1 (408) are inserted into the slide groove 2 (419). The fixed shaft 1 (408) forms a limit fit with the connecting arm (417) through the slide groove 2 (419).

4. A tissue cell extraction and collection device according to claim 3, characterized in that: The side of the collar (406) is fixedly connected to a limiting block (420); the side of the fixing seat (404) close to the limiting block (420) is fixedly connected to a mounting seat (421); the side of the mounting seat (421) close to the limiting block (420) is fixedly mounted with an electric telescopic rod (422); and the movable shaft of the electric telescopic rod (422) is fixedly connected to the limiting block (420).

5. A tissue cell extraction and collection device according to claim 4, characterized in that: The top of the oscillating plate (402) is detachably connected to a test tube rack (423).

6. A tissue cell extraction and collection device according to claim 1, characterized in that: The base (1) is provided with a filter lifting assembly, the filter lifting assembly comprising a gear (501) fixedly connected to the output shaft of the dual-axis motor (403) on the side away from the fixed seat one (404), the bottom of the turntable (3) is fixedly connected to a bevel gear (502), the gear (501) is meshingly connected with the bevel gear (502), the top of the base (1) is fixedly connected to a fixed frame (503), the side of the fixed frame (503) close to the rotating seat (2) is fixedly connected to a fixed seat two (504), the fixed seat two (504) ) is rotatably connected to the bottom of the rotating disk (3), the nut (505) is internally threadedly connected to a lead screw (506), the top of the lead screw (506) passes through a second fixing seat (504), the top of the rotating disk (3) is fixedly connected to a connecting frame (507), there are no less than two connecting frames (507), the two connecting frames (507) are symmetrically arranged on the rotating disk (3), the top ends of the two connecting frames (507) are respectively fixedly connected to the two sides of the nut (505), and the bottom end of the lead screw (506) is fixedly connected to a connecting rod (508).

7. A tissue cell extraction and collection device according to claim 6, characterized in that: The length of the lead screw (506) is the same as the distance between the second fixing seat (504) and the test tube rack (423).

8. A tissue cell extraction and collection device according to claim 7, characterized in that: A filter assembly is disposed at the bottom of the connecting rod (508), and the filter assembly includes a fixing ring (601) fixedly connected to the connecting rod (508). A cell filter (602) is sleeved on the bottom of the connecting rod (508). A connecting seat (603) is fixedly connected to the top of the cell filter (602). There are no less than two connecting seats (603), and the two connecting seats (603) are symmetrically arranged on the cell filter (602). The fixing rods (601) are fixedly connected to the two sides of the fixing ring (601). 04), a sliding groove (605) is provided on the side of the two fixing rods (604) away from the fixing ring (601), and an insertion rod (606) is slidably connected in the two sliding grooves (605), and a spring (607) is arranged in the two sliding grooves (605), and a slot (608) is provided on the side of the two connecting seats (603) close to the fixing ring (601), and the two insertion rods (606) are respectively engaged with the two slots (608), and a shifting block (609) is respectively sleeved on the two insertion rods (606).

9. A tissue cell extraction and collection device according to claim 8, characterized in that: The middle of the cell filter (602) has a socket, and the bottom of the connecting rod (508) forms a sliding fit with the cell filter (602) through the socket.

10. The tissue cell extraction and collection device according to claim 9, characterized in that: A sealing gasket is provided at the connection between the slide groove (605) and the insertion rod (606).

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