High-throughput environment variable culture box, culture device and culture method

Abstract

The invention discloses a high-throughput environment variable culture box, a culture device and a culture method, and belongs to the technical field of microorganism culture. The high-throughput environment variable incubator comprises a shell, a plurality of frameworks are arranged in the shell in a linear array mode, a plurality of containing holes for containing culture tubes are formed in the frameworks in the length direction of the frameworks, and heat insulation pads are arranged between the frameworks and between the frameworks and the shell. Each framework is internally and independently provided with a heating structure for heating the corresponding culture tube, a lamp strip is arranged on the base at the bottom of the shell, the lamp strips are located under the placing holes, and the lamp strips are perpendicular to the frameworks. By adopting the high-throughput environment variable culture box, the culture device and the culture method, the problems that an existing culture box is single in simulated environment variable and cannot realize cross environment variable culture can be solved, and the high-throughput environment variable culture box has the advantages of safety and convenience in liquid relief and convenience in operation.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial cultivation, and in particular to a high-throughput environmental variable incubator, a cultivation device and a cultivation method. Background Art

[0002] A microbial incubator is a device specially designed to provide a controlled environment to promote the growth of microorganisms. Incubators are essential for fields such as biological research, medical testing, and food industry quality control. According to different needs, the microbial incubator can adjust the internal lighting, temperature, humidity, and even gas composition (such as carbon dioxide concentration) to meet the optimal growth conditions for specific microorganisms. In the process of studying microbial culture, it is necessary to study the effects of external environmental changes such as temperature and light on microbial growth. Existing incubators can generally only meet the cultivation of microorganisms under one combination of environmental conditions at a time. To study the effects of a series of environmental condition changes or different combinations of environmental variables on microbial culture, it is necessary to set up multiple sets of tests and conduct multiple cultures, which leads to low test efficiency and increased uncertainty in test results. Summary of the invention

[0003] The purpose of the present invention is to provide a high-throughput environmental variable incubator, a culture device and a culture method, which solve the problem that the existing incubator simulates a single environmental variable and cannot realize cross-environmental variable culture, and has the advantages of safe and convenient pipetting and easy operation.

[0004] To achieve the above-mentioned objectives, the present invention provides a high-throughput environmental variable incubator, comprising a shell, a plurality of frames are arranged in a linear array inside the shell, a plurality of placement holes for placing culture tubes are opened on the frames along the length direction of the frames, thermal insulation pads are arranged between the frames and between the frames and the shells, a heating structure for heating the culture tubes is independently arranged inside each frame, a light strip is arranged on the base at the bottom of the shell, the light strip is located directly below the placement hole, and the light strip is arranged vertically to the frames.

[0005] Preferably, the placement hole is a structure with openings at both ends, a limiting plate is provided at the bottom of the placement hole, an opening is provided in the middle of the limiting plate for inserting the bottom of the culture tube, and the aperture of the opening is smaller than the outer diameter of the culture tube.

[0006] Preferably, the heating structure includes a heating plate and a temperature control probe, which are respectively arranged on partitions on both sides of the skeleton. The skeleton is made of high thermal conductivity material, and a temperature control chip and a light control chip are arranged on the base. The temperature control chip is electrically connected to the temperature control probe and the digital display control device, and the light control chip is electrically connected to the light strip and the digital display control device. The digital display control device is arranged outside the shell.

[0007] Preferably, a cover body is arranged above the shell, a gear is rotatably arranged on the shell, a second motor is arranged on the shell to drive the gear to rotate, a rack meshing with the gear is arranged on the cover body, and a guide rail is arranged on the top of the shell to guide the sliding of the cover body.

[0008] Preferably, a battery for powering the incubator is disposed on the base, and a Bluetooth module is disposed on the base, and the Bluetooth module is electrically connected to the digital display control device.

[0009] A culture device containing the above-mentioned high-throughput environmental variable culture box includes a storage room for placing the culture box, an operating table is arranged on the outer side of the storage room, and a pipetting mechanism is arranged above the operating table.

[0010] Preferably, the storage chamber comprises a frame with at least one side open, a plurality of partitions are arranged in parallel up and down inside the frame, a plurality of first guide seats are arranged on the partitions, the first guide seats divide the partitions into a plurality of placement areas for placing incubators, a slope gradually tilted inward from top to bottom is arranged on the side of the first guide seat close to the placement area, a plurality of first support plates supporting the incubators are arranged above the partitions, a gap is arranged between the first support plates for inserting the shovel into the bottom of the incubator, a first moving module for driving the shovel to move between the storage chamber and the operating table is arranged on the frame, a first wireless power supply module is arranged on the frame, a wireless power supply module receiving end compatible with the first wireless power supply module is arranged on the shell of the incubator, and the wireless power supply module receiving end is electrically connected to the digital display control device arranged on the shell.

[0011] Preferably, the operating table is provided with a second guide seat for dividing the operating table into several operating areas, a slope gradually tilted inward from top to bottom is provided on the side of the second guide seat close to the operating area, a second support plate supporting the incubator is provided at the bottom of the operating area, a second wireless power supply module is provided on the operating area, a wireless power supply module receiving end compatible with the second wireless power supply module is provided on the shell of the incubator, and the wireless power supply module receiving end is electrically connected to a digital display control device provided on the shell; a second mobile module is provided on the operating table, and the pipetting mechanism is provided on the second mobile module through a card slot.

[0012] Preferably, the pipetting mechanism includes a pipette, which is clamped on the clamping slot, a piston is provided inside the pipette, the piston is sealingly and slidably connected to the inner wall of the pipette, a screw is provided inside the pipette to drive the piston to rise and fall, the screw is rotatably connected to the piston, the screw is threadedly connected to the top cover on the top of the pipette, a first motor that drives the screw to rotate is provided at the top of the screw, a needle is provided at the bottom of the pipette, a heating coil is provided inside the needle; an embedded water cavity is provided on the side wall of the pipette, the water cavity is connected to the bottom of the internal cavity of the pipette, the water cavity is connected to a water inlet provided on the side wall of the pipette, the water inlet is connected to a circulating pump through a water inlet pipe, the circulating pump is connected to a clean water tank through a connecting pipe, the circulating pump and the clean water tank are both provided on an operating table, a sewage tank is provided on the operating table, and a drain outlet for discharging water from the sewage tank is provided on the operating table.

[0013] The culture method based on the above culture device comprises the following steps: S1, placing the incubator on the operating table, powering the incubator through the second wireless power supply module on the operating table and the wireless power supply module receiving end on the shell, and inserting the culture tube into the placement hole of the skeleton; S2, driving the pipette to move above the culture tube through the second moving module, and the needle passes through the sealing plug above the culture tube to transfer liquid into the culture tube; S3, the pipette is moved to the top of the sewage tank by the second moving module, and the circulating pump sends the clean water in the clean water tank into the water cavity through the connecting pipe and the water inlet pipe to clean the piston, the pipette and the inside of the needle. After cleaning, the sewage is stored in the sewage tank, and the heating coil heats and disinfects the needle; S4. After the transfer is completed, the top opening of the incubator is closed through the shell, the first mobile module drives the shovel to move above the operating table, the shovel is inserted into the bottom of the incubator from between the second support plates, the first mobile module lifts the incubator through the shovel, the incubator leaves the operating table, the shovel puts the incubator into the placement area of ​​the storage room, the shovel is inserted into the placement area from between the first support plates, the incubator is placed on the first support plate, and the first wireless power supply module supplies power to the incubator through the wireless power supply module receiving end; S5. Set the temperature and light intensity in the incubator through the digital display control device and carry out cultivation.

[0014] Therefore, the advantages and positive effects of the high-throughput environmental variable incubator, culture device and culture method of the present invention are: 1. A skeleton is set in the incubator, and the skeletons are separated by insulation pads. A heating plate and a temperature control probe are set inside the skeleton to facilitate independent temperature setting for each skeleton, which is conducive to studying the culture conditions under different temperature conditions.

[0015] 2. An LED light strip is provided on the base at the bottom of the shell. The LED light strip is located directly below the placement hole to provide light for the culture tube. Since the LED light strip is vertically arranged with the frame, the incubator forms a chessboard structure. Each culture tube is under different temperature and light conditions, which is convenient for culture research under high-throughput environmental variable conditions.

[0016] 3. The frame of the present invention is provided with a first wireless power supply module, and the housing of the incubator is provided with a wireless power supply module receiving end, and the incubator located in the placement area is powered by the first wireless power supply module. The operation area is provided with a second wireless power supply module, and the incubator located in the operation area is powered by the second wireless power supply module. The incubator itself is provided with a battery to ensure the stability of the power supply of the incubator.

[0017] 4. The side wall of the pipette is provided with an embedded water cavity, which is used to clean the piston, pipette and needle with clean water to avoid contamination of the next culture tube to be pipetted. A heating coil is provided inside the needle to heat and disinfect the needle to avoid contamination of the culture solution.

[0018] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of an incubator according to an embodiment of the present invention; Figure 2 The cross-sectional structure of the incubator according to the embodiment of the present invention is shown in FIG. Figure 1 ; Figure 3 The cross-sectional structure of the incubator according to the embodiment of the present invention is shown in FIG. Figure 2 ; Figure 4 This is a schematic diagram of the structure of a culture device according to an embodiment of the present invention; Figure 5 A schematic diagram of a cross-sectional structure of a storage chamber according to an embodiment of the present invention; Figure 6 This is a schematic diagram of the back structure of the storage chamber according to an embodiment of the present invention; Figure 7 This is a schematic diagram of the structure of an operating table according to an embodiment of the present invention; Figure 8 It is a schematic diagram of the local structure of the operating table of an embodiment of the present invention; Fig. 9 Schematic diagram of the structure of the liquid transfer mechanism according to an embodiment of the present invention.

[0020] Reference numerals 1. Storage room; 11. Frame; 12. Partition; 13. Placement area; 14. First guide seat; 15. First support plate; 16. First wireless power supply module; 17. First mobile module; 18. Shovel; 2. Operation table; 21. Operation area; 22. Second guide seat; 23. Second support plate; 24. Second wireless power supply module; 25. Circulation pump; 26. Clean water tank; 27. Sewage tank; 28. Connecting pipe; 29. ​​Water inlet pipe; 210. Drain port; 211. Second mobile module; 212. Card slot; 3. Liquid transfer mechanism; 31. Liquid transfer tube; 32. Top cover; 33. Screw; 34. First motor; 35. Piston; 36. Needle; 37. Water chamber; 38. Water inlet; 39. Heating coil; 4. Incubator; 41. Shell; 42. Cover; 43. Guide rail; 44. Gear; 45. Rack; 46. Second motor; 47. Frame; 48. Placement hole; 49. Insulation pad; 410. Culture tube; 411. Sealing plug; 412. Heating plate; 413. Temperature control probe; 414. LED light strip; 415. Limiting plate; 416. Wireless power supply module receiving end; 417. Battery; 418. Bluetooth module; 419. Temperature control chip; 420. Light control chip; 421. Digital display control device; 422. Base. DETAILED DESCRIPTION

[0021] In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invented product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "setting", "installation", and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0022] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0023] like Figure 1 , Figure 2 , Figure 3As shown. A high-throughput environmental variable incubator includes a shell 41, which is a rectangular structure with an open top. The interior of the shell 41 is provided with a plurality of long strip-shaped skeletons 47 in a linear array, and the skeleton 47 is provided with a plurality of placement holes 48 for placing culture tubes 410 along the length direction of the skeleton 47, and the aperture of the placement hole 48 is slightly larger than the outer diameter of the culture tube 410. The placement hole 48 is a structure with openings at both ends, and a limiting plate 415 is fixedly provided at the bottom of the placement hole 48. An opening is provided in the middle of the limiting plate 415 for inserting the bottom of the culture tube 410, and the aperture of the opening is smaller than the outer diameter of the culture tube 410. The limiting plate 415 improves the stability of the culture tube 410 when placed in the shell 41, and makes the bottom end of the culture tube 410 protrude from the limiting plate 415, so as to facilitate illumination of the culture tube 410.

[0024] Insulation pads 49 are provided between the skeletons 47 and between the skeleton 47 and the shell 41. The insulation pads 49 separate the adjacent skeletons 47 to reduce the mutual influence of the temperature between the adjacent skeletons 47, so as to facilitate the independent setting and control of the temperature on each skeleton 47. A heating structure for heating the culture tube 410 is independently provided inside each skeleton 47. The heating structure includes a heating plate 412 and a temperature control probe 413, which are respectively fixed on the partitions 12 on both sides of the skeleton 47.

[0025] The heating plate 412 is used to heat the skeleton 47 and provide a set temperature for each skeleton 47, so as to facilitate the study of the culture conditions at different temperatures. The temperature control probe 413 is used to detect the temperature of the skeleton 47. When the temperature of the skeleton 47 is higher than the set value, the power of the heating plate 412 is lowered or the heating plate 412 is turned off through the digital display control device 421; when the temperature of the skeleton 47 is lower than the set value, the power of the heating plate 412 is increased or the heating plate 412 is turned on through the digital display control device 421; so that the temperature of the skeleton 47 is maintained at the set temperature, and the stability of the temperature of the skeleton 47 is improved.

[0026] The skeleton 47 is made of a material with high thermal conductivity to improve the effect of heat conduction and the uniformity of the temperature of the culture tubes 410 located on the same skeleton 47 .

[0027] A light strip is provided on the base 422 at the bottom of the housing 41. The light strip is located directly below the placement hole 48 and is vertically arranged with the frame 47. The light strip is an LED light strip 414, which is used to provide light for the culture tube 410. Since the LED light strip 414 is vertically arranged with the frame 47, the incubator 4 forms a chessboard structure, and each culture tube 410 is under different temperature and different light conditions, which is convenient for culture research under high-throughput environmental variable conditions.

[0028] A temperature control chip 419 and a light control chip 420 are provided on the base 422. The temperature control chip 419 is electrically connected to the temperature control probe 413 and the digital display control device 421. The light control chip 420 is electrically connected to the light strip and the digital display control device 421. The digital display control device 421 is provided outside the housing 41. The digital display control device 421 adopts an existing structure. The digital display control device 421, the light control chip 420, the temperature control chip 419, the LED light strip 414, the temperature probe, and the heating plate 412 are all connected using existing technologies as needed.

[0029] A cover 42 is disposed above the housing 41. The housing 41 is rotatably provided with a gear 44 through a bearing. The housing 41 is provided with a second motor 46 that drives the gear 44 to rotate. A rack 45 that meshes with the gear 44 is fixedly disposed on the cover 42. The cover 42 slides along the housing 41 through the gear 44 and the rack 45, thereby closing or opening the top opening of the housing 41. A guide rail 43 that guides the sliding of the cover 42 is fixedly disposed at the top of the housing 41.

[0030] The base 422 is provided with a battery 417 for powering the incubator 4. The base 422 is provided with a Bluetooth module 418, which is electrically connected to the digital display control device 421 using existing technology as required. The incubator 4 is connected to a computer or a portable mobile device through the Bluetooth module 418.

[0031] like Figure 4 A culture device containing the high-throughput environmental variable culture box 4 includes a storage room 1 for placing the culture box 4, an operating table 2 is arranged on one side of the storage room 1, and a pipetting mechanism 3 is arranged above the operating table 2. The culture tube 410 on the operating table 2 is pipetted by the pipetting mechanism 3.

[0032] like Figure 5 , Figure 6 As shown. The storage room 1 includes a frame 11 with at least one side open, and a plurality of partitions 12 are fixedly arranged in parallel up and down inside the frame 11. A plurality of first guide seats 14 are fixedly arranged on the partition 12, and the first guide seats 14 divide the partition 12 into a plurality of placement areas 13 for placing the incubator 4. The bottom size of the placement area 13 is slightly larger than the size of the incubator 4, so that the incubator 4 can be placed just in the placement area 13. An inclined surface gradually inclined inward from top to bottom is provided on the side of the first guide seat 14 close to the placement area 13, and the incubator 4 is positioned by the inclined surface, so that the incubator 4 can be stably and accurately placed in the placement area 13. A plurality of first support plates 15 supporting the incubator 4 are fixedly arranged above the partition 12. A gap is provided between the first support plates 15 to allow the shovel 18 to be inserted into the bottom of the incubator 4, so that the incubator 4 can be placed in or out of the placement area 13 by the shovel 18.

[0033] The frame 11 is provided with a first moving module 17 for driving the shovel 18 to move between the storage room 1 and the operating table 2. The first moving module 17 adopts an existing structure and can realize the three-dimensional movement of the shovel 18 on the X, Y, and Z axes. The frame 11 is provided with a first wireless power supply module 16. The shell 41 of the incubator 4 is provided with a wireless power supply module receiving end 416 adapted to the first wireless power supply module 16. The wireless power supply module receiving end 416 is electrically connected to a digital display control device 421 provided on the shell 41. The incubator 4 located in the placement area 13 is powered by the first wireless power supply module 16.

[0034] like Figure 7 , Figure 8 As shown. A second guide seat 22 is fixedly provided on the operating table 2 to divide the operating table 2 into a plurality of operating areas 21. A slope gradually tilted inward from top to bottom is provided on the side of the second guide seat 22 close to the operating area 21, so as to facilitate the accurate placement of the incubator 4 into the operating area 21. A second support plate 23 supporting the incubator 4 is fixedly provided at the bottom of the operating area 21. The shovel 18 passes between the second support plates 23 to take the incubator 4 out of the operating area 21. A second wireless power supply module 24 is provided on the operating area 21, and the incubator 4 located in the operating area 21 is powered by the second wireless power supply module 24.

[0035] The operating table 2 is provided with a second movable module 211, and the liquid transfer mechanism 3 is provided on the second movable module 211 through a slot 212. The second movable module 211 adopts an existing structure, and can realize three-dimensional movement of the liquid transfer mechanism 3 on the X, Y, and Z axes.

[0036] like Fig. 9 The pipetting mechanism 3 includes a pipette 31, which is clamped on the clamping groove 212. A piston 35 is arranged inside the pipette 31, and the piston 35 is a rubber plug. The piston 35 is sealed and slidably connected with the inner wall of the pipette 31, and is used to suck or squeeze the culture liquid into or out of the pipette 31. A screw 33 is arranged inside the pipette 31 to drive the piston 35 to rise and fall, and the screw 33 is rotatably connected with the piston 35 through a bearing. The screw 33 is threadedly connected with the top cover 32 at the top of the pipette 31, and a first motor 34 is arranged at the top of the screw 33 to drive the screw 33 to rotate. The first motor 34 drives the piston 35 to move up and down through the screw 33. A needle 36 is arranged at the bottom of the pipette 31, and the needle 36 can penetrate the sealing plug 411 above the culture tube 410. The sealing plug 411 is made of elastic rubber material, and its sealing property is maintained after the needle 36 is pulled out, so as to prevent the culture tube 410 from being contaminated by the outside. A heating coil 39 is disposed inside the needle 36 , and the heating coil 39 heats and sterilizes the needle 36 .

[0037] An embedded water cavity 37 is provided on the side wall of the pipette 31, and the water cavity 37 is communicated with the bottom of the internal cavity of the pipette 31. The water cavity 37 is communicated with a water inlet 38 provided on the side wall of the pipette 31. The water inlet 38 is connected to the circulation pump 25 through the water inlet pipe 29, and the circulation pump 25 is connected to the clean water tank 26 through the connecting pipe 28. The circulation pump 25 and the clean water tank 26 are both arranged on the operating table 2. A sewage tank 27 is provided on the operating table 2, and a drain port 210 for discharging water from the sewage tank 27 is provided on the operating table 2. The clean water in the clean water tank 26 enters the water cavity 37 through the connecting pipe 28 and the water inlet pipe 29, and cleans the piston 35, the pipette 31 and the needle 36 to avoid contamination of the next culture tube 410 for pipetting.

[0038] The culture method based on the above culture device comprises the following steps: S1. Place the incubator 4 on the operating table 2, power the incubator 4 through the second wireless power supply module 24 on the operating table 2 and the wireless power supply module receiving end 416 on the shell 41, and insert the culture tube 410 into the placement hole 48 of the skeleton 47.

[0039] S2. The second moving module 211 drives the pipette 31 to move above the culture tube 410 , and the needle 36 passes through the sealing plug 411 above the culture tube 410 to transfer liquid into the culture tube 410 .

[0040] S3. Move the pipette 31 to the top of the sewage tank 27 through the second movable module 211, and the circulation pump 25 sends the clean water in the clean water tank 26 into the water cavity 37 through the connecting pipe 28 and the water inlet pipe 29 to clean the piston 35, the pipette 31, and the inside of the needle 36. After cleaning, the sewage is stored in the sewage tank 27, and the heating coil 39 heats and disinfects the needle 36.

[0041] S4. After the transfer is completed, the top opening of the incubator 4 is closed through the housing 41. The first moving module 17 drives the shovel 18 to move above the operating table 2, and the shovel 18 is inserted from between the second support plates 23 to the bottom of the incubator 4. The first moving module 17 lifts the incubator 4 through the shovel 18, and the incubator 4 leaves the operating table 2. The shovel 18 puts the incubator 4 into the placement area 13 of the storage room 1, and the shovel 18 is inserted from between the first support plates 15 to the placement area 13, and the incubator 4 is placed on the first support plate 15. The first wireless power supply module 16 supplies power to the incubator 4 through the wireless power supply module receiving end 416.

[0042] S5. Set the temperature and light in the incubator 4 through the digital display control device 421 to carry out cultivation.

[0043] Therefore, the use of the high-throughput environmental variable incubator, the culture device and the culture method described in the present invention can solve the problem that the existing incubator cannot realize cross-environmental variable culture, and has the advantages of safe and convenient pipetting and easy operation.

[0044] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A high-throughput environmental variable incubator, characterized in that: It includes a shell, and a plurality of frames are arranged in a linear array inside the shell. A plurality of placement holes for culture tubes are opened on the frames along the length direction of the frames. Insulation pads are arranged between the frames and between the frames and the shell. A heating structure for heating the culture tubes is independently arranged inside each frame. A light strip is arranged on the base at the bottom of the shell. The light strip is located directly below the placement hole and is arranged vertically to the frames.

2. A high-throughput environmental variable incubator according to claim 1, characterized in that: The placement hole is an open structure at both ends, a limiting plate is arranged at the bottom of the placement hole, an opening is arranged in the middle of the limiting plate for inserting the bottom of the culture tube, and the aperture of the opening is smaller than the outer diameter of the culture tube.

3. A high-throughput environmental variable incubator according to claim 1, characterized in that: The heating structure includes a heating plate and a temperature control probe, which are respectively arranged on the partitions on both sides of the skeleton. The skeleton is made of high thermal conductivity material. A temperature control chip and a light control chip are arranged on the base. The temperature control chip is electrically connected to the temperature control probe and the digital display control device. The light control chip is electrically connected to the light strip and the digital display control device. The digital display control device is arranged outside the shell.

4. A high-throughput environmental variable incubator according to claim 1, characterized in that: A cover body is arranged above the shell, a gear is rotatably arranged on the shell, a second motor is arranged on the shell to drive the gear to rotate, a rack meshing with the gear is arranged on the cover body, and a guide rail is arranged on the top of the shell to guide the sliding of the cover body.

5. The high-throughput environmental variable incubator according to claim 1, characterized in that: The base is provided with a battery for supplying power to the incubator, and the base is provided with a Bluetooth module, which is electrically connected to the digital display control device.

6. A culture device comprising the high-throughput environmental variable incubator according to any one of claims 1 to 5, characterized in that: The utility model comprises a storage room for placing the incubator, an operation table is arranged on one side outside the storage room, and a liquid transfer mechanism is arranged above the operation table.

7. A culture device according to claim 6, characterized in that: The storage room includes a frame with at least one side open, a plurality of partitions are arranged in parallel up and down inside the frame, a plurality of first guide seats are arranged on the partitions, the first guide seats divide the partitions into a plurality of placement areas for placing incubators, a slope gradually tilted inward from top to bottom is arranged on the side of the first guide seat close to the placement area, a plurality of first support plates supporting the incubators are arranged above the partitions, gaps are arranged between the first support plates for inserting the shovel into the bottom of the incubator, a first moving module for driving the shovel to move between the storage room and the operating table is arranged on the frame, a first wireless power supply module is arranged on the frame, a wireless power supply module receiving end compatible with the first wireless power supply module is arranged on the shell of the incubator, and the wireless power supply module receiving end is electrically connected to a digital display control device arranged on the shell.

8. A culture device according to claim 6, characterized in that: The operating table is provided with a second guide seat which divides the operating table into a plurality of operating areas; a slope which gradually tilts inward from top to bottom is provided on the side of the second guide seat close to the operating area; a second support plate which supports the incubator is provided at the bottom of the operating area; a second wireless power supply module is provided on the operating area; a wireless power supply module receiving end which is compatible with the second wireless power supply module is provided on the shell of the incubator; the wireless power supply module receiving end is electrically connected to a digital display control device provided on the shell; a second mobile module is provided on the operating table; and a pipetting mechanism is provided on the second mobile module through a card slot.

9. A culture device according to claim 6, characterized in that: The pipetting mechanism includes a pipette, which is clamped on a clamping slot, a piston is arranged inside the pipette, the piston is sealingly and slidingly connected to the inner wall of the pipette, a screw is arranged inside the pipette to drive the piston to rise and fall, the screw is rotationally connected to the piston, the screw is threadedly connected to the top cover on the top of the pipette, a first motor that drives the screw to rotate is arranged on the top of the screw, a needle is arranged at the bottom of the pipette, a heating coil is arranged inside the needle; an embedded water cavity is arranged on the side wall of the pipette, the water cavity is communicated with the bottom of the internal cavity of the pipette, the water cavity is communicated with a water inlet arranged on the side wall of the pipette, the water inlet is connected to a circulation pump through a water inlet pipe, the circulation pump is connected to a clean water tank through a connecting pipe, the circulation pump and the clean water tank are both arranged on an operating table, a sewage tank is arranged on the operating table, and a drain outlet for discharging water from the sewage tank is arranged on the operating table.

10. A cultivation method based on a cultivation device according to any one of claims 7 to 9, characterized in that: The following steps are involved: S1, placing the incubator on the operating table, powering the incubator through the second wireless power supply module on the operating table and the wireless power supply module receiving end on the shell, and inserting the culture tube into the placement hole of the skeleton; S2, driving the pipette to move above the culture tube through the second moving module, and the needle passes through the sealing plug above the culture tube to transfer liquid into the culture tube; S3, the pipette is moved to the top of the sewage tank by the second moving module, and the circulating pump sends the clean water in the clean water tank into the water cavity through the connecting pipe and the water inlet pipe to clean the piston, the pipette and the inside of the needle. After cleaning, the sewage is stored in the sewage tank, and the heating coil heats and disinfects the needle; S4. After the transfer is completed, the top opening of the incubator is closed through the shell, the first mobile module drives the shovel to move above the operating table, the shovel is inserted into the bottom of the incubator from between the second support plates, the first mobile module lifts the incubator through the shovel, the incubator leaves the operating table, the shovel puts the incubator into the placement area of ​​the storage room, the shovel is inserted into the placement area from between the first support plates, the incubator is placed on the first support plate, and the first wireless power supply module supplies power to the incubator through the wireless power supply module receiving end; S5. Set the temperature and light intensity in the incubator through the digital display control device and carry out cultivation.

Images