Rapid point selection method capable of being used for LA-ICP-MS experiment

Abstract

The invention provides a rapid point selection method capable of being used for an LA-ICP-MS experiment, and belongs to the technical field of laser ablation. The rapid point selection method capable of being used for the LA-ICP-MS experiment comprises the following steps: scanning a probe sheet by adopting a Raman spectrum, and marking to obtain a picture with marked point positions; transmitting the photo with the marked point location to a transfer station, and transmitting the received information to a laser ablation system by the transfer station; calibrating the picture with the marked point location and the picture of the same unmarked sample to be detected; overlapping the picture with the marked point location and the picture of the same unmarked sample to be detected to form a whole image; and quickly positioning the pre-denudation point according to the marked point location. According to the rapid point selection method, the specific position of the preset point is rapidly positioned through the Raman spectrum and the laser ablation system, and the efficiency of the LA-ICP-MS experiment is improved.

Description

Technical Field

[0001] The invention relates to the technical field of laser ablation, and in particular to a rapid point selection method that can be used for LA-ICP-MS experiments. Background Art

[0002] Raman spectroscopy is a type of scattering spectrum. Raman spectroscopy is based on the Raman scattering effect discovered by Indian scientist CV Raman. It analyzes the scattering spectrum with different frequencies from the incident light to obtain information on molecular vibration and rotation, and is applied to the study of molecular structure. Raman spectroscopy technology is rich in information, simple sample preparation, low interference from water, and is not affected by the form of sample substances. In addition, Raman spectroscopy analysis has unique advantages such as no damage to samples, rapid analysis, low maintenance cost, and simple use. It is widely used in chemistry, materials, physics, polymers, biology, medicine, geology and other fields.

[0003] Laser Ablation-Inductively Coupled Plasma Mass Spectrometer (LA-ICP-MS) is a combined instrument based on the laser ablation system (LA) and inductively coupled plasma mass spectrometer (ICP-MS). With the gradual maturity of the laser ablation system, laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) as a solid direct sampling and micro-area analysis technology is widely used in geology, environment, biology, materials, industrial product testing and other fields. It can analyze major, trace, trace and ultra-trace elements, especially in rare earth elements, PGEs, isotope analysis and other aspects.

[0004] The selection of the type of mineral to be tested and the specific location requires the joint efforts of a scanning electron microscope, a polarizing microscope and an ordinary microscope. During the LA-ICP-MS experiment, the local area under the target lens of the laser ablation system is usually slowly searched by comparing the marked photos of the specific location of the mineral to be tested. This method is cumbersome to operate, consumes a lot of time and manpower, and has extremely low experimental efficiency.

[0005] Therefore, providing a rapid point selection method that can be used in LA-ICP-MS experiments has important practical significance. Summary of the invention

[0006] The purpose of the present invention is to provide a rapid point selection method that can be used for LA-ICP-MS experiments, aiming to solve the technical problems of complicated operation steps and low experimental efficiency in finding pre-erosion points in existing LA-ICP-MS experiments.

[0007] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0008] The present invention provides a rapid point selection method that can be used for LA-ICP-MS experiments, comprising the following steps:

[0009] Use a Raman microscope to scan the probe sheet under a 5x low-power microscope to obtain an optical image, find the point to be tested in the optical image, align the cross cursor with the point to be tested under a 50x high-power microscope to test, and obtain the Raman spectrum. Determine the mineral type based on the Raman peak position displayed in the spectrum, click and hold the left mouse button to drag the Raman spectrum to the optical image, and obtain a picture with marked spectrum collection points;

[0010] Transmitting the picture with the marked spectrum collection points to a transfer station, and the transfer station transmits the received information to the laser ablation system;

[0011] Calibrate the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested;

[0012] Superimposing the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested to form a whole image;

[0013] Quickly locate the pre-erosion points based on the marked points.

[0014] Furthermore, the scanning of the probe sheet is specifically scanning the entire probe sheet.

[0015] Furthermore, the mark is a cross mark.

[0016] Furthermore, the transfer station is specifically an intermediate information processing part between the Raman spectroscopy and laser ablation systems, and is used to temporarily store information in various formats such as files, texts, and pictures.

[0017] Furthermore, the calibration is specifically processed on the ActiveView2 software matched with the NWR193 excimer laser ablation sampling system.

[0018] Furthermore, the superposition method is to place the two pictures in different layers and form a whole image by superimposing them up and down.

[0019] Furthermore, the rapid positioning is specifically to rapidly locate the pre-ablation point according to the marked point under the target lens of the laser ablation system.

[0020] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0021] 1. The present invention analyzes the proportion of mineral elements through Raman spectroscopy to determine the mineral category, avoiding the cumbersome operation of using a scanning electron microscope, a polarizing microscope and an ordinary microscope for identification, saving a lot of time, manpower and financial resources;

[0022] 2. The present invention marks the position of the point in advance on the Raman spectrum, which is convenient for the laser ablation system to quickly locate the specific position of the preset point, effectively shortening the time of searching for the specific position of the preset point under the target lens in the prior art, and improving the experimental efficiency of the LA-ICP-MS experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The figure is a schematic diagram of the process of the rapid point selection method for LA-ICP-MS experiment according to the present invention. DETAILED DESCRIPTION

[0024] The present invention provides a rapid point selection method that can be used for LA-ICP-MS experiments, comprising the following steps:

[0025] Use a Raman microscope to scan the probe sheet under a 5x low-power microscope to obtain an optical image, find the point to be tested in the optical image, align the cross cursor with the point to be tested under a 50x high-power microscope to test, and obtain the Raman spectrum. Determine the mineral type based on the Raman peak position displayed in the spectrum, click and hold the left mouse button to drag the Raman spectrum to the optical image, and obtain a picture marking the spectrum collection point;

[0026] Transmitting the image with the marked spectrum collection points to a transfer station, and the transfer station transmits the received information to the laser ablation system;

[0027] Calibrate the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested;

[0028] Superimposing the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested to form a whole image;

[0029] Quickly locate the pre-erosion points based on the marked points.

[0030] The probe sheet is scanned by a Raman microscope to mark and obtain a photo with the marked points;

[0031] The present invention scans the probe sheet through a Raman microscope, and can analyze the types of mineral elements according to the scanning results, and then mark them; the Raman microscope of the present invention can scan the entire probe sheet, and the operation is simple, time-saving and efficient.

[0032] In the present invention, the test conditions for obtaining the Raman spectrum are preferably 600-line grating, spectral window center position 2150 rel.cm-1, 532nm laser, laser power 15mW, integration time 5s, integration times 8 times, 50x objective lens.

[0033] In the present invention, the scanning probe sheet is preferably a whole probe sheet. The probe sheet of the present invention is a probe sheet for LA-ICP-MS analysis, with a thickness of about 50 to 80 um, wherein the size of the carrier glass is 2.5*5 cm 2 .

[0034] In the present invention, the mark is a cross mark.

[0035] The image with the cross marking the spectrum collection point is transmitted to the transfer station, and the transfer station transmits the received information to the laser ablation system;

[0036] The present invention establishes a transfer station between the Raman spectrum and the laser ablation system, performs secondary processing and integration on the received information, and then transmits the integrated information to the laser ablation system. This process has fast data transmission speed, high data security, and convenient resource sharing.

[0037] In the present invention, the transfer station is specifically an intermediate information processing part between the Raman spectroscopy and laser ablation systems, and is used to temporarily store information in various formats such as files, texts, and pictures.

[0038] Calibrate the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested;

[0039] The present invention pre-calibrates the image with marked spectrum collection points before selecting points, thereby improving the accuracy of point selection and further improving the accuracy of the experiment.

[0040] In the present invention, the calibration is preferably processed on the ActiveView2 software matched with the NWR193 excimer laser ablation sampling system.

[0041] Superimposing the photograph with the marked points and the unmarked picture of the same sample to be tested to form a whole image;

[0042] In the present invention, the superposition method is to place two pictures in different layers and form a whole image by superimposing them up and down.

[0043] The stacking method of the present invention has the following advantages: first, the layers can be edited independently without affecting each other; second, when multiple layers are stacked, the lower layers can be displayed through the transparency area of ​​the upper layer to form a whole image.

[0044] Quickly locate the pre-erosion points based on the marked points.

[0045] In the present invention, the rapid positioning is specifically to rapidly locate the pre-ablation point according to the marked point under the target lens of the laser ablation system.

[0046] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0047] Example 1

[0048] (1) Use a Raman microscope to scan the entire probe sheet under a low-power microscope (5x) to obtain an optical image, find the point to be tested in the optical image, and align the cross cursor with the point to be tested under a high-power microscope (50x) for testing. The test conditions are as follows: 600-line grating, spectral window center position 2150 rel.cm-1, 532nm laser, laser power 15mW, integration time 5s, integration times 8 times, 50x objective lens, test to obtain Raman spectrum, determine the mineral type according to the Raman peak position displayed in the spectrum, click and hold the left mouse button to drag the Raman spectrum to the optical image, and obtain a picture with a cross marking the spectrum collection point;

[0049] (2) transmitting the image with the cross-marked spectrum collection point to a transfer station, which performs secondary processing and integration on the received information and transmits the processed information to the laser ablation system;

[0050] (3) Calibrate the obtained image with the cross-marked spectrum collection point and the unmarked image of the same sample to be tested using diagonal lines on ActiveView2;

[0051] (4) superimposing the picture with the cross-marked spectrum collection point and the unmarked picture of the same sample to be tested in a top-to-bottom stacking manner to form a whole image;

[0052] (5) Under the target lens of the laser ablation system, the pre-ablation point is quickly located according to the marked point.

[0053] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A rapid point selection method for LA-ICP-MS experiments, characterized in that: The following steps are involved: Use a Raman microscope to scan the probe sheet under a 5x low-power microscope to obtain an optical image, find the point to be tested in the optical image, align the cross cursor with the point to be tested under a 50x high-power microscope to test, and obtain the Raman spectrum. Determine the mineral type based on the Raman peak position displayed in the spectrum, click and hold the left mouse button to drag the Raman spectrum to the optical image, and obtain a picture with marked spectrum collection points; Transmitting the picture with the marked spectrum collection points to a transfer station, and the transfer station transmits the received information to the laser ablation system; Calibrate the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested; Superimposing the picture with the marked spectrum collection points and the unmarked picture of the same sample to be tested to form a whole image; Quickly locate the pre-erosion points based on the marked points.

2. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The scanning of the probe sheet specifically involves scanning the entire probe sheet.

3. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The mark is a cross mark.

4. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The transfer station is specifically an intermediate information processing part between the Raman spectrum and the laser ablation system, and is used to temporarily store information in various formats such as files, texts, and pictures.

5. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The calibration is specifically processed on the ActiveView2 software matched with the NWR193 excimer laser ablation sampling system.

6. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The superposition method is to place the two pictures on different layers and form a whole image by superimposing them up and down.

7. The rapid site selection method for LA-ICP-MS experiment according to claim 1, characterized in that: The rapid positioning is specifically to rapidly locate the pre-ablation point according to the marked point under the target lens of the laser ablation system.

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