Multi-channel electrochemical chip detection components and detection equipment

Abstract

The present invention relates to a multi-channel electrochemical chip detection component and a detection device, wherein the detection component comprises a multi-channel electrochemical chip and a liquid confinement unit; the multi-channel electrochemical chip comprises a substrate and an electrode arranged on the substrate, the substrate comprises a transverse base, the base has comb-tooth portions which are spaced and protrude from the same transverse edge of the base, each comb-tooth portion is provided with an electrode to form mutually independent comb-tooth electrode units; the liquid confinement unit comprises a reaction pool, the reaction pool can be mutually aligned with the comb-tooth electrode unit to insert the comb-tooth electrode unit for reaction; the liquid confinement unit comprises a cleaning pool, the cleaning pool can be mutually aligned with the comb-tooth electrode unit to insert the comb-tooth electrode unit for cleaning. The detection component simplifies the complexity of the automated equipment; moreover, each comb-tooth electrode unit is independent of each other, and multiple detections can be performed on multiple analytes.

Description

Technical Field

[0001] The present invention relates to the field of chip technology, and in particular to a multi-channel electrochemical chip detection component. The present invention also relates to a detection device using the detection component for detection. Background Art

[0002] Electrochemical chips are generally composed of a substrate and electrodes on it. The substrate is generally made of non-conductive glass, ceramics, plastics and other materials, and the electrodes on it are generally metals, conductive metal oxides and non-metallic coatings. There are generally three areas on the electrochemical chip, the electrode area, the wire area and the lead area. When in use, drop the liquid to be tested on the electrode area, and use a connector to connect to the contact pad on the chip lead area to derive the signal. An electrode area on the chip is generally composed of 2-4 electrodes and is called a cell.

[0003] In order to achieve high-throughput electrochemical analysis, multiple cells are often placed on an electrochemical chip, and each cell independently runs electrochemical analysis and produces corresponding electrochemical reactions. This type of chip is called a multi-channel electrochemical chip or a multiplexed detection electrochemical chip.

[0004] When using electrochemical analysis chips, biological detection chips, modified electrode chips, etc., it is often necessary to drip liquid on a specific area on the chip surface. After the components in the liquid undergo physical / chemical reactions with the chip surface, the component molecules in the liquid will adhere to the electrode surface to form a molecularly modified electrode. For multi-channel electrochemical chips, the modifiers and reactants of each independent cell will be different, so the reaction liquids between each cell are required to be isolated from each other.

[0005] All cells of the multi-channel electrochemical chip are distributed in the same plane. During detection, the chip needs to maintain a horizontal upward posture. The isolation layer and the surface tension of the liquid between cells form independent droplets on each cell. Due to the surface tension, the droplets remain on the surface of the cell and do not interfere with each other.

[0006] Existing multi-channel electrochemical chips can be divided into two types in terms of isolation function: one is a horizontal multi-channel electrode with an isolation layer, and the other is a horizontal multi-channel electrode with an isolation cavity.

[0007] Among them, horizontal multi-channel electrodes with isolation layers are the most common. They usually process multi-electrode cells on the same substrate material, and then cover the electrodes and the substrate with a layer of plastic film as an isolation layer. The core commonality of this type of multi-channel electrochemical chip is that during the operation process, including electrode modification and electrochemical detection, the chip always remains horizontal to ensure that the reaction solution is maintained on each independent electrode. Therefore, there are the following disadvantages:

[0008] 1) This type of chip only uses surface tension to form droplets of reaction liquid and maintain them on the electrode surface. The droplets are prone to overflow during movement, resulting in cross-reactions. In addition, the cleaning solution cannot clean each electrode independently during cleaning. The unreacted analytes in each channel may contact other cells while being taken away from the electrode by the cleaning solution, resulting in cross-reactions.

[0009] 2) During the incubation process, all electrodes are in contact with the reaction solution, which easily leads to nonspecific adhesion in the non-working electrode area.

[0010] 3) All electrodes are on the same plane, and the flux per unit area is small.

[0011] 4) The incubation process relies on the diffusion rate of the reactants in the droplets to the electrode surface, and stirring cannot be introduced.

[0012] 5) Due to open cleaning, a separate closed space needs to be set up in the equipment to clean the chip, and it is easy to produce residues on the front and back surfaces of the chip. In addition, excessive gas pressure will also affect the loss of surface modification molecules.

[0013] 6) A pipetting system is required to add the reaction solution to the electrode surface. Some chips require the operation of tiny droplets (4-10ul), and the system cost is high.

[0014] The horizontal multi-channel electrode with an isolation cavity tightly connects the planar electrode with a porous structure of a certain depth, forming a well structure with a certain depth on the surface of each cell, which is similar to installing a well plate on the planar electrode. There is an electrode cell at the bottom of each hole of the well plate. During the incubation process, all electrodes are in contact with the reaction solution, and nonspecific adhesion is still easy to occur in the non-working electrode area. Moreover, all electrodes are on the same plane, and the flux per unit area is still small. Summary of the invention

[0015] The object of the present invention is to provide a multi-channel electrochemical chip detection component to at least solve part of the above technical problems.

[0016] Another object of the present invention is to provide a detection device using the multi-channel electrochemical chip detection component.

[0017] To achieve the above-mentioned purpose, the multi-channel electrochemical chip detection component provided by the present invention includes a multi-channel electrochemical chip and a liquid confinement unit; the multi-channel electrochemical chip includes a substrate and an electrode arranged on the substrate, the substrate includes a transverse base, the base has comb-tooth portions that are spaced and protrude from the same transverse edge of the base, and each of the comb-tooth portions is provided with an electrode to form independent comb-tooth electrode units; the liquid confinement unit includes a reaction tank for accommodating a reaction liquid, the reaction tank is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for reaction; the liquid confinement unit includes a cleaning tank for accommodating a cleaning liquid, the cleaning tank is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for cleaning.

[0018] To achieve the above-mentioned purpose, the multi-channel electrochemical chip detection component provided by the present invention includes a multi-channel electrochemical chip and a liquid confinement unit; the multi-channel electrochemical chip includes at least two substrates, the substrate includes a lateral base, the base has comb-tooth portions that are spaced and protrude from the same lateral edge of the base; the corresponding comb-tooth portions are respectively provided with different partial electrodes to form a comb-tooth electrode unit in combination; the liquid confinement unit includes a reaction pool for accommodating reaction liquid, the reaction pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for reaction; the liquid confinement unit includes a cleaning pool for accommodating cleaning liquid, the cleaning pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode for cleaning.

[0019] Optionally, the multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with a first electrode, and the comb-teeth portion of the second substrate is provided with a second electrode and a third electrode;

[0020] Alternatively, the multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with the first electrode and the third electrode, and the comb-teeth portion of the second substrate is provided with the second electrode;

[0021] Alternatively, the multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with a first electrode and a second electrode, and the comb-teeth portion of the second substrate is provided with a third electrode;

[0022] Alternatively, the multi-channel electrochemical chip comprises three substrates, wherein the comb-teeth of the first substrate are provided with the first electrode, the comb-teeth of the second substrate are provided with the second electrode, and the comb-teeth of the third substrate are provided with the third electrode.

[0023] Optionally, at least some of the electrodes are arranged in a reaction pool of the liquid confinement unit for detection.

[0024] Optionally, part of the electrodes are provided in the form of plug-ins in the reaction pool of the liquid confinement unit for detection, or part of the electrodes are directly formed on the material surface of the reaction pool of the liquid confinement unit for detection.

[0025] Optionally, the comb teeth portion and the base portion are a split connection structure, and both are made of the same material or different materials.

[0026] Optionally, the comb teeth portion is detachably connected to the base portion.

[0027] Optionally, the base comprises a mechanical device for inserting the comb teeth in an arranged combination.

[0028] Optionally, the reaction pool includes a plurality of grooves located on the same substrate and capable of accommodating different reaction liquids; the cleaning pool includes a plurality of grooves located on the same substrate and capable of accommodating cleaning liquids.

[0029] Optionally, the reaction pool and the cleaning pool are arranged and distributed along the same direction on the substrate according to the order of the detection process.

[0030] Optionally, some reaction pools in the same detection sequence are merged into one reaction pool, and the rest of the reaction pools in the same detection sequence are independent of each other; and / or, some cleaning pools in the same detection sequence are merged into one cleaning pool, and the rest of the cleaning pools in the same detection sequence are independent of each other.

[0031] Optionally, at least one group of the reaction cells is a reaction cell that contains a reaction solution and is inserted into the comb-shaped electrode unit for detection.

[0032] To achieve the above another object, the present invention provides a multi-channel electrochemical chip detection device, which uses any of the multi-channel electrochemical chip detection components described above for detection, including:

[0033] A liquid confinement unit bearing portion, used for bearing the liquid confinement unit with the opening upward;

[0034] The chip operation unit is used to carry and move the multi-channel electrochemical chip with the comb-teeth portion downward, so that the comb-teeth electrode units of the multi-channel electrochemical chip react and clean in the reaction pool and the cleaning pool of the liquid confinement unit according to the detection process.

[0035] To achieve the above another object, the present invention provides a multi-channel electrochemical chip detection device, which uses any of the multi-channel electrochemical chip detection components described above for detection, including:

[0036] A chip carrying portion, used for carrying the multi-channel electrochemical chip with the comb teeth facing downward;

[0037] The liquid confinement unit operating part is used to carry and move the liquid confinement unit with the opening upward, so that the comb-shaped electrode unit of the multi-channel electrochemical chip reacts and is cleaned in the reaction pool and the cleaning pool of the liquid confinement unit according to the detection process.

[0038] The existing multi-channel electrochemical chip is both a liquid confinement unit and an electrode unit. When the reaction liquid needs to be replaced, the liquid must be separated from the chip by some method, such as a liquid suction device or a flushing and drying device.

[0039] Different from this, the multi-channel electrochemical chip detection component provided by the present invention has a liquid restriction unit and a multi-channel electrochemical chip with modified electrodes as different independent units. By arranging the electrodes on a substrate with comb teeth, an electrode array is formed in which each comb tooth has a group or one electrode. The electrode array may be composed of one substrate containing all electrodes in the electrode group, or multiple substrates, each of which contains a chip group composed of one or more electrodes. The purpose of separating the modified electrode from the reaction liquid is achieved by separating the liquid restriction unit (reaction container) from the modified electrode. When in use, the modification and detection of the multi-channel electrode is completed by the cooperation of the multi-channel electrochemical chip and the liquid restriction unit. Since different reaction pools can accommodate a variety of different reaction solutions, the modified reaction solution can be completely contacted with the modified electrode by moving the electrode alone or the liquid restriction unit alone, without the need to operate tiny droplets and separate droplets, which simplifies the complexity of the automated equipment; moreover, each comb-shaped electrode unit is independent of each other, and multiple analytes can be detected.

[0040] On this basis, another multi-channel electrochemical chip detection component provided by the present invention can increase the number of working electrodes per unit area, improve detection flux and reduce costs by separating any non-working electrodes from the working electrodes; since the working electrodes between channels are completely isolated, the working electrode of each channel is isolated from other electrodes, which can eliminate cross-reactions and nonspecific adhesion.

[0041] In addition, the above-mentioned multi-channel electrochemical chip detection component can add agitation by moving and rotating the liquid confinement unit or the working electrode along the X, Y, and Z directions to increase the reaction rate, thereby improving the detection sensitivity within a certain reaction time. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 A schematic diagram of the structure of a multi-channel electrochemical chip detection component provided in the first embodiment of the present invention;

[0043] Figure 2 for Figure 1 A schematic diagram of the detection principle of a single comb-shaped electrode unit of a multi-channel electrochemical chip detection component is shown;

[0044] Figure 3 A schematic diagram of the structure of a multi-channel electrochemical chip detection component provided in a second embodiment of the present invention;

[0045] Figure 4 A schematic diagram of the structure of a multi-channel electrochemical chip detection component provided in the third embodiment of the present invention;

[0046] Figure 5 A schematic diagram of the structure of a multi-channel electrochemical chip detection assembly provided in a fourth embodiment of the present invention;

[0047] Figure 6 A schematic diagram of the structure of a multi-channel electrochemical chip detection assembly provided in a fifth embodiment of the present invention;

[0048] Figure 7 for Figure 1 A schematic diagram of the detection process of the multi-channel electrochemical chip detection component shown;

[0049] Figure 8 for Figure 4 Schematic diagram of the detection process of the multi-channel electrochemical chip detection component shown.

[0050] In the figure:

[0051] 10-multi-channel electrochemical chip; 20-liquid confinement unit; 11-substrate; 111-base; 112-comb tooth portion; 12-electrode; 121-first electrode; 122-second electrode; 123-third electrode;

[0052] 21-reaction tank; 22-cleaning tank; 23-substrate.

[0053] A-first reaction tank; B-first cleaning tank; C-second cleaning tank; D-second reaction tank; E-third cleaning tank; F-third reaction tank. DETAILED DESCRIPTION

[0054] In order to enable those skilled in the art to better understand the solution of the present invention, the present invention is further described in detail below in conjunction with the accompanying drawings and specific implementation methods.

[0055] In this article, the terms "upper, lower, inside, outside" and the like are established based on the positional relationships shown in the drawings. The corresponding positional relationships may also change depending on the drawings, and therefore cannot be understood as absolute limitations on the scope of protection; moreover, relational terms such as "first" and "second" and the like are merely used to distinguish one component from another with the same name, and do not necessarily require or imply any actual relationship or order between these components.

[0056] Please refer to Figure 1 , Figure 1 This is a schematic structural diagram of a multi-channel electrochemical chip detection component provided in the first embodiment of the present invention.

[0057] As shown in the figure, in a specific embodiment, the multi-channel electrochemical chip detection component provided by the present invention is mainly composed of two parts: a multi-channel electrochemical chip 10 and a liquid confinement unit 20 for matching use.

[0058] The multi-channel electrochemical chip 10 has a substrate 11 and electrodes 12 arranged on the substrate 11, wherein the substrate 11 is in a long strip shape and has a laterally extending base 111, the base 111 has four comb-tooth portions 112 that are spaced apart and protrude from the same lateral edge of the base 111, each comb-tooth portion 112 is respectively provided with a first electrode 121, a second electrode 122 and a third electrode 123, all electrodes 12 are on the surface of a substrate 11, thereby forming four independent comb-tooth-shaped electrode units, that is, each comb-tooth portion 112 is an electrode cell.

[0059] The liquid confinement unit 20 has a plurality of rows of reaction pools 21 ( Figure 1 Only one row is shown), each row of reaction cells 21 has four reaction cells 21, which can be aligned with the comb-tooth electrode units of the multi-channel electrochemical chip 10 so as to be inserted into the comb-tooth electrode units for reaction.

[0060] Similarly, the liquid confinement unit 20 also has multiple rows of cleaning pools 22 for containing cleaning liquid. The number of cleaning pools 22 in each row can be four, which can be aligned with the comb-tooth electrode units of the multi-channel electrochemical chip 10 to insert the comb-tooth electrode units for cleaning. Through cleaning, excess substances can be prevented from affecting the reaction in the next binding process.

[0061] The reaction pool 21 and the cleaning pool 22 are located on the same substrate 23, and the two are arranged and distributed along the same direction on the substrate 23 according to the order of the detection process. Different reaction pools can be used to accommodate the same or different reaction liquids, and different cleaning pools can be used to accommodate the same or different cleaning liquids. At least one group of reaction pools 21 (for example, the last row of reaction pools) is used to accommodate the reaction liquid and insert the comb-shaped electrode unit for detection.

[0062] Moreover, if Figure 7 , Figure 8 As shown, some of the reaction pools 21 in the same detection sequence can be combined into one reaction pool, and the remaining reaction pools 21 in the same detection sequence are independent of each other; some of the cleaning pools 22 in the same detection sequence can be combined into one cleaning pool, and the remaining cleaning pools 22 in the same detection sequence are independent of each other.

[0063] The reaction pool 21 and the cleaning pool 22 are in the shape of grooves with openings facing upwards, and are semi-enclosed cavities. They can be pre-sealed with a covering film, and when in use, the film can be removed by tearing or the like to expose the opening. The reaction liquid or cleaning liquid can be pre-injected into the interior, or the reaction liquid or cleaning liquid can be injected into the interior after opening.

[0064] The following uses a three-electrode electrochemical chip as an example to explain the detection principle of the comb-shaped multi-channel electrode:

[0065] like Figure 2 As shown, the electrode 12 of each comb-shaped electrode unit is composed of a first electrode 121, a second electrode 122 and a third electrode 123. The target object to be measured can be combined with the first electrode through one or more steps of reaction, and the information of the object to be measured can be determined by the electrical signal generated by the detection system.

[0066] Please refer to Figure 3 , Figure 3 A schematic structural diagram of a multi-channel electrochemical chip detection assembly provided in the second embodiment of the present invention.

[0067] As shown in the figure, compared with the first embodiment, the difference of this embodiment is:

[0068] Since the liquid confinement unit 20 and the multi-channel electrochemical chip 10 are different independent units, the modification and detection of the multi-channel electrode are completed through the cooperation of the multi-channel electrochemical chip 10 and the liquid confinement unit 20 during use.

[0069] Therefore, the second electrode and the third electrode 123 may no longer be a ring-shaped structure, but may be designed as a linear structure, which can also achieve the purpose of the present invention.

[0070] In this embodiment, the same parts as those in the first embodiment are given the same figure marks, and the same text descriptions are omitted.

[0071] Please continue to refer to Figure 4 , Figure 4 A schematic structural diagram of a multi-channel electrochemical chip detection component provided in the third embodiment of the present invention.

[0072] As shown in the figure, compared with the first embodiment, the difference of this embodiment is:

[0073] The multi-channel electrochemical chip 10 has two substrates 11, wherein the comb-tooth portion 112 of the first substrate is provided with a first electrode 121, and the comb-tooth portion 112 of the second substrate is provided with a second electrode 122 and a third electrode 123. The corresponding comb-tooth portions 112 on the two substrates 11 are combined to form a comb-tooth electrode unit. When in use, the corresponding comb-tooth portions 112 of the two substrates 11 can be inserted into the reaction cell 21 or the cleaning cell 22 together to form a three-electrode system for modification and detection.

[0074] In this embodiment, the same parts as those in the first embodiment are given the same figure marks, and the same text descriptions are omitted.

[0075] Please refer to further Figure 5 , Figure 5 A schematic structural diagram of a multi-channel electrochemical chip detection assembly provided in the fourth embodiment of the present invention.

[0076] As shown in the figure, compared with the first embodiment, the difference of this embodiment is:

[0077] The multi-channel electrochemical chip 10 has two substrates 11, wherein the comb-tooth portion 112 of the first substrate is provided with a first electrode 121 and a second electrode 122, and the comb-tooth portion 112 of the second substrate is provided with a third electrode 123. The corresponding comb-tooth portions 112 on the two substrates 11 are combined to form a comb-tooth electrode unit. When in use, the corresponding comb-tooth portions 112 of the two substrates 11 can be inserted into the reaction pool 21 and the cleaning pool 22 together to form a three-electrode system for modification and detection.

[0078] Of course, in other embodiments, the first electrode 121 and the third electrode 123 may be disposed on the comb-tooth portion 112 of the first substrate, and the second electrode 122 may be disposed on the comb-tooth portion 112 of the second substrate.

[0079] In this embodiment, the same parts as those in the first embodiment are given the same figure marks, and the same text descriptions are omitted.

[0080] Please continue to refer to Figure 6 , Figure 6 A schematic structural diagram of a multi-channel electrochemical chip detection assembly provided in the fifth embodiment of the present invention.

[0081] As shown in the figure, compared with the first embodiment, the difference of this embodiment is:

[0082] The multi-channel electrochemical chip 10 has three substrates 11, wherein the comb-tooth portion 112 of the first substrate is provided with a first electrode 121, the comb-tooth portion 112 of the second substrate is provided with a second electrode 122, and the comb-tooth portion 112 of the third substrate is provided with a third electrode 123. That is, each electrode 12 is on a substrate 11, and three substrates 11 are used at the same time, and the corresponding comb-tooth portions 112 form a three-electrode system.

[0083] In this embodiment, the same parts as those in the first embodiment are given the same figure marks, and the same text descriptions are omitted.

[0084] In the second to fifth embodiments, since the first electrode 121 and other electrodes are processed on two or more substrates 11 respectively, it can be seen that the detection flux is significantly improved under the premise that the area of ​​the substrate 11 is the same.

[0085] Please refer to Figure 7 , Figure 7 for Figure 1 Schematic diagram of the detection process of the multi-channel electrochemical chip detection component shown.

[0086] Taking a certain detection process as an example, the liquid restriction unit 20 is provided with a first reaction pool A, a first cleaning pool B, a second cleaning pool C, a second reaction pool D, a third cleaning pool E and a third reaction pool F, wherein the first reaction pool A is used to accommodate the first reagent, the first cleaning pool B is two independent cleaning pools 22, the second cleaning pool C is merged into an integral cleaning pool 22, the second reaction pool D is merged into an integral reaction pool 21, which is used to accommodate the second reagent, and similarly, the third cleaning pool E is also merged into an integral cleaning pool 22.

[0087] When in use, the two comb-shaped electrode units of the multi-channel electrochemical chip 10 are inserted into the first reaction tank A, the first cleaning tank B, the second cleaning tank C, the second reaction tank D, and the third cleaning tank E in sequence, the film (covering the other areas except the first electrode 121) is removed, and finally the current signal is detected in the third reaction tank F.

[0088] It should be noted here that for different detection processes, the design of the cleaning pool 22 can be multiple or one, which depends entirely on the needs of the detection. The separation and merging are also determined based on whether there is a need to prevent cross contamination between each electrode 12. If cross contamination needs to be prevented, the cleaning pool 22 needs to be designed separately. If there is no risk of cross contamination, it can be designed as a combined cleaning pool 22. The purpose of designing two consecutive cleaning pools 22 is to ensure sufficient cleaning. The risk of cross contamination after the first cleaning is reduced. Therefore, the second cleaning can be combined.

[0089] If required by the test, the physical form of the cleaning pool 22 and the cleaning solution in each cleaning pool 22 can be flexibly changed.

[0090] Please refer to Figure 8 , Figure 8 for Figure 4 Schematic diagram of the detection process of the multi-channel electrochemical chip detection component shown.

[0091] Taking a certain detection process as an example, the liquid confinement unit 20 is provided with a first reaction pool A, a first cleaning pool B, a second cleaning pool C, a second reaction pool D, a third cleaning pool E and a third reaction pool F containing sample molecules, wherein the first reaction pool A is used to accommodate the first reagent, the first cleaning pool B is two independent cleaning pools 22, the second cleaning pool C is merged into an integral cleaning pool 22, the second reaction pool D is merged into an integral reaction pool 21, which is used to accommodate the second reagent. Similarly, the third cleaning pool E is also merged into an integral cleaning pool 22.

[0092] When in use, the comb-tooth portion 112 of the multi-channel electrochemical chip with the first electrode is inserted into the first reaction tank A, the first cleaning tank B, the second cleaning tank C, the second reaction tank D, and the third cleaning tank E in sequence, the film (covering the other area except the first electrode 121) is removed, and then the comb-tooth portion 112 with the first electrode and the comb-tooth portion 112 with other electrodes are inserted into the third reaction tank F together to detect the current signal.

[0093] When a multi-channel electrochemical chip 10 having multiple substrates 11 is used, it is necessary to control the relative positions and distances between the substrates 11. Specifically, this can be fixed by designing the reaction pool 21, or it can be accomplished by an auxiliary device that controls the movement of the substrates 11. For example, a robotic arm-like device can be used to fix the substrates 11 and move them up and down and forward and backward, and the relative positions between the substrates 11 can be controlled by controlling the robotic arm.

[0094] The above embodiments are only preferred solutions of the present invention and are not limited thereto. On this basis, targeted adjustments can be made according to actual needs to obtain different implementation methods.

[0095] For example, part of the electrodes are provided in the form of plug-ins in the reaction pool of the liquid confinement unit for detection, or part of the electrodes are directly formed on the material surface of the reaction pool of the liquid confinement unit for detection.

[0096] Alternatively, the comb-teeth portion 112 and the base portion 111 of the chip may be two separate parts. The base portion 111 and the comb-teeth portion 112 may be made of different materials, for example, the base portion 111 is plastic, the comb-teeth portion 112 is a glass or silicon substrate, or both the base portion 111 and the comb-teeth portion 112 are made of plastic, etc. The comb-teeth portion 112 may be fixed to the base portion 111 in the form of an insert, so that the combination is more flexible.

[0097] Moreover, in addition to the above-mentioned forms, the base 111 can also adopt a mechanical device as the base, and the comb teeth 112 are directly inserted into the mechanical device in the form of an arrangement and combination, or the mechanical device or the separate base 111 can be designed to clamp the comb teeth 112 of the electrode in the form of a clip.

[0098] Alternatively, in addition to the three-electrode system, the electrode 12 may also be a four-electrode system or a five-electrode system, or the electrode may be in other shapes such as square, rectangle, triangle, etc. Since there are many possible implementation methods, they will not be described one by one here.

[0099] To achieve the above another object, the present invention provides a multi-channel electrochemical chip detection device, which uses the multi-channel electrochemical chip detection component described above for detection, including:

[0100] A liquid confinement unit carrying portion, used for carrying a liquid confinement unit 20 with an opening upward;

[0101] The chip operation part is used to carry and move the multi-channel electrochemical chip 10 with the comb-tooth part 112 facing downward, so that the comb-tooth electrode units of the multi-channel electrochemical chip 10 are inserted into and withdrawn from the reaction pool 21 and the cleaning pool 22 according to the detection process, and moved between the reaction pool 21 and the cleaning pool 22 for modification and detection.

[0102] To achieve the above another object, the present invention provides a multi-channel electrochemical chip detection device, which uses the multi-channel electrochemical chip detection component described above for detection, including:

[0103] The chip carrying portion is used to carry the multi-channel electrochemical chip 10 with the comb teeth 112 facing downward;

[0104] The liquid confinement unit operating part is used to carry and move the liquid confinement unit 20 with the opening facing upward, so that the comb-shaped electrode unit of the multi-channel electrochemical chip 10 can be inserted into and withdrawn from the reaction pool 21 and the cleaning pool 22 according to the detection process, and moved between the reaction pool 21 and the cleaning pool 22 for modification and detection.

[0105] The present invention has the following beneficial effects:

[0106] 1) By separating the liquid confinement unit 20 from the multi-channel electrochemical chip 10, the two become independent units. The modified reaction liquid can be completely contacted with the modified electrode only by moving the electrode alone or the liquid confinement unit 20 alone. There is no need to operate tiny droplets and separate droplets, which simplifies the complexity of the automated equipment.

[0107] 2) The chip substrate 11 has a tooth-like structure, and the multiple comb-tooth electrode units are independent of each other. Each comb-tooth electrode unit can be detected independently. The liquid confinement unit 20 has one or more grooves for accommodating a certain volume of liquid. The grooves can accommodate a certain volume of reaction liquid. The grooves corresponding to each comb-tooth electrode unit can be loaded with different reaction liquids. The comb-tooth electrode units of the chip can be inserted into the grooves of the liquid confinement unit 20 to achieve multiple detections of multiple analytes.

[0108] 3) Separate any non-working electrode from the working electrode. The working electrode can be independent or combined with one or more non-working electrodes. The non-working electrode can be an independent unit or integrated with the reaction container. The non-working electrode and the working electrode are in contact with the same reaction solution at least once or multiple times at the same time, thereby increasing the number of working electrodes per unit area, thereby increasing the detection throughput and reducing costs.

[0109] 4) The working electrodes between channels are completely isolated, and the working electrodes of each channel are isolated from other electrodes. Different analytes or samples have independent reaction pools and working electrodes, and the non-working electrodes do not directly contact the reaction system, eliminating cross-reactions and nonspecific adhesion.

[0110] 5) The multi-channel electrochemical chip detection component can add agitation by moving and rotating the liquid confinement unit or the working electrode in the X, Y, and Z directions to increase the reaction rate, thereby improving the detection sensitivity within a certain reaction time.

[0111] The above is a detailed introduction to the multi-channel electrochemical chip detection component and detection equipment provided by the present invention. Specific examples are used in this article to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the core idea of ​​the present invention. It should be pointed out that for ordinary technicians in this technical field, without departing from the principles of the present invention, the present invention can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A multi-channel electrochemical chip detection component, characterized in that: It comprises a multi-channel electrochemical chip and a liquid confinement unit; the multi-channel electrochemical chip comprises a substrate and an electrode arranged on the substrate, the substrate comprises a transverse base, the base has comb-tooth portions which are spaced apart and protrude from the same transverse edge of the base, each comb-tooth portion is provided with an electrode to form mutually independent comb-tooth electrode units; the liquid confinement unit comprises a reaction pool for accommodating reaction liquid, the reaction pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for reaction; the liquid confinement unit comprises a cleaning pool for accommodating cleaning liquid, the cleaning pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for cleaning.

2. A multi-channel electrochemical chip detection component, characterized in that: It comprises a multi-channel electrochemical chip and a liquid confinement unit; the multi-channel electrochemical chip comprises at least two substrates, the substrate comprises a lateral base, the base has comb-tooth portions which are spaced apart and protrude from the same lateral edge of the base; the corresponding comb-tooth portions are respectively provided with different partial electrodes to form a comb-tooth electrode unit in combination; the liquid confinement unit comprises a reaction pool for accommodating reaction liquid, the reaction pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode unit for reaction; the liquid confinement unit comprises a cleaning pool for accommodating cleaning liquid, the cleaning pool is constructed to be able to align with the comb-tooth electrode unit of the multi-channel electrochemical chip so as to insert the comb-tooth electrode for cleaning.

3. The multi-channel electrochemical chip detection component according to claim 2, characterized in that: The multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with a first electrode, and the comb-teeth portion of the second substrate is provided with a second electrode and a third electrode; Alternatively, the multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with the first electrode and the third electrode, and the comb-teeth portion of the second substrate is provided with the second electrode; Alternatively, the multi-channel electrochemical chip comprises two substrates, wherein the comb-teeth portion of the first substrate is provided with a first electrode and a second electrode, and the comb-teeth portion of the second substrate is provided with a third electrode; Alternatively, the multi-channel electrochemical chip comprises three substrates, wherein the comb-teeth of the first substrate are provided with the first electrode, the comb-teeth of the second substrate are provided with the second electrode, and the comb-teeth of the third substrate are provided with the third electrode.

4. The multi-channel electrochemical chip detection component according to claim 2 or 3, characterized in that: At least part of the electrodes are arranged in a reaction pool for detection in the liquid confinement unit.

5. The multi-channel electrochemical chip detection component according to claim 4, characterized in that: Some electrodes are arranged in the form of plug-ins in the reaction pool for detection in the liquid confinement unit, or some electrodes are directly formed on the material surface of the reaction pool for detection in the liquid confinement unit.

6. The multi-channel electrochemical chip detection component according to claim 1 or 2, characterized in that: The comb teeth part and the base part are a separate connection structure, and the two are made of the same material or different materials.

7. The multi-channel electrochemical chip detection component according to claim 6, characterized in that: The comb tooth portion is detachably connected to the base portion.

8. The multi-channel electrochemical chip detection component according to claim 1 or 2, characterized in that: The base includes a mechanism for inserting the comb teeth in an arrayed combination.

9. The multi-channel electrochemical chip detection component according to claim 1 or 2, characterized in that: The reaction pool includes a plurality of grooves located on the same substrate and capable of accommodating different reaction liquids; the cleaning pool includes a plurality of grooves located on the same substrate and capable of accommodating cleaning liquids.

10. The multi-channel electrochemical chip detection component according to claim 9, characterized in that: The reaction pool and the cleaning pool are arranged and distributed along the same direction on the substrate according to the order of the detection process.

11. The multi-channel electrochemical chip detection component according to claim 10, characterized in that: Some reaction pools in the same detection sequence are combined into one reaction pool, and the rest of the reaction pools in the same detection sequence are independent of each other; and / or some cleaning pools in the same detection sequence are combined into one cleaning pool, and the rest of the cleaning pools in the same detection sequence are independent of each other.

12. The multi-channel electrochemical chip detection component according to claim 1 or 2, characterized in that: At least one group of the reaction cells is a reaction cell that contains reaction liquid and is inserted into the comb-shaped electrode unit for detection.

13. A multi-channel electrochemical chip detection device, using the multi-channel electrochemical chip detection component according to any one of claims 1 to 12 for detection, characterized in that: include: A liquid confinement unit bearing portion, used for bearing the liquid confinement unit with the opening upward; The chip operation unit is used to carry and move the multi-channel electrochemical chip with the comb-teeth portion downward, so that the comb-teeth electrode units of the multi-channel electrochemical chip react and clean in the reaction pool and the cleaning pool of the liquid confinement unit according to the detection process.

14. A multi-channel electrochemical chip detection device, using the multi-channel electrochemical chip detection component according to any one of claims 1 to 12 for detection, characterized in that: include: A chip carrying portion, used for carrying the multi-channel electrochemical chip with the comb teeth facing downward; The liquid confinement unit operating part is used to carry and move the liquid confinement unit with the opening upward, so that the comb-shaped electrode unit of the multi-channel electrochemical chip reacts and is cleaned in the reaction pool and the cleaning pool of the liquid confinement unit according to the detection process.

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