Bactericidal gynecological lotion containing composite lysozyme

Abstract

The invention relates to a bactericidal gynecological lotion containing composite lysozyme, and belongs to the technical field of gynecological nursing products, the gynecological lotion comprises the following raw materials: a biological membrane removal concentrated solution, a composite lysozyme concentrated solution, recombinant human collagen, a humectant, a gelatinizing agent, essence, a solubilizer, a preservative, a pH regulator and purified water. According to the invention, lysozyme and lysostaphin are compounded to form a composite lysozyme concentrated solution as an antibacterial component of the system, and meanwhile, a nonionic surfactant and a zwitterionic surfactant are compounded, so that the finished product effectively has an excellent bactericidal effect. In addition, the added biological membrane removing concentrated solution provides the capacity of disintegrating a biological membrane, an effective foundation is laid for the composite lysozyme concentrated solution, recombinant human collagen is added for nursing, and a humectant is added for improving the wettability and assisting in deep maintenance. The prepared finished product can synergistically achieve the effects of film cleaning, sterilization and maintenance.

Description

Technical Field

[0001] The invention belongs to the technical field of gynecological care products, and in particular relates to a bactericidal gynecological lotion containing composite lysozyme. Background Art

[0002] Women's private parts are the most delicate and fragile skin. If they do not pay attention to maintenance in daily life, it may cause bacterial infection, leading to abnormal vaginal discharge, dryness, odor, itching and other symptoms. A healthy vaginal environment has a weakly acidic pH of 3.8-4.5. The maintenance of this ecosystem environment is mainly due to the presence of vaginal lactobacilli. When external environmental interference or internal factors cause an imbalance in the vaginal microbial flora, the normal environment of the vagina changes, pathogens proliferate, and cause vaginal infection. Bacterial vaginosis (BV) is the most common imbalance of vaginal flora. The Centers for Disease Control and Prevention estimates that 65% of human bacterial infections are related to biofilms. Studies have shown that microbial films composed of multiple microorganisms are found in 90% of patients with vaginitis.

[0003] In the treatment of vaginal diseases, antibiotics or chemical antibacterial agents are still the main treatment methods, especially antibiotics. However, in fact, antibiotics cannot have a sustained control effect on bacterial microbial films, let alone adjust the balance of vaginal microorganisms. In addition, antibiotics can also destroy beneficial bacteria and easily cause users to develop drug resistance, resulting in poor use effects.

[0004] Therefore, it is of great practical significance to overcome the above-mentioned deficiencies and provide a safe and effective gynecological lotion, especially a gynecological lotion with the dual care effects of removing bacterial biofilm and effectively killing bacteria, and effectively reducing the recurrence rate and adverse side effects. Summary of the invention

[0005] The purpose of the present invention is to provide a bactericidal gynecological wash containing composite lysozyme, which solves the problems of membrane cleaning, bactericidal and maintenance effects existing in the prior art.

[0006] The purpose of the present invention can be achieved through the following technical solutions: A bactericidal gynecological lotion containing composite lysozyme, the gynecological lotion comprising the following raw materials: Biofilm removal concentrate, complex lysozyme concentrate, recombinant human collagen, moisturizer, fragrance, solubilizer, preservative, pH adjuster and purified water.

[0007] As a preferred technical solution of the present invention, the gynecological lotion also includes a gelling agent.

[0008] Furthermore, the gelling agent includes any one or two of hydroxypropyl methylcellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone and poloxamer.

[0009] As a preferred technical solution of the present invention, the biofilm removal concentrate includes a nonionic surfactant and a zwitterionic surfactant.

[0010] As a more preferred technical solution of the present invention, the mass ratio of the nonionic surfactant to the zwitterionic surfactant is 1:1-9.

[0011] Further, the nonionic surfactant includes at least one of alkyl glycosides, fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers and sorbitan fatty acid esters; Furthermore, the alkyl glycosides include alkyl glycoside APG0810 and alkyl glycoside 1214; the fatty alcohol polyoxyethylene ethers include laureth-4 and laureth-23; the alkylphenol polyoxyethylene ethers include nonylphenol polyoxyethylene ether; and the sorbitan fatty acid ester includes PEG-80.

[0012] Furthermore, the nonionic surfactant is preferably alkyl polyglycoside APG0810.

[0013] Furthermore, the zwitterionic surfactant includes cocamidopropyl betaine and lauramidopropyl betaine.

[0014] Furthermore, the zwitterionic surfactant is preferably lauramidopropyl betaine.

[0015] As a preferred technical solution of the present invention, the preparation method of the biofilm removal concentrate is: The nonionic surfactant and the zwitterionic surfactant are mixed evenly to obtain a biofilm removal concentrate.

[0016] As a preferred technical solution of the present invention, the composite lysozyme concentrate comprises lysozyme, lysostaphin and purified water.

[0017] Furthermore, the mass ratio of the lysozyme, lysostaphin and purified water is 5-20:0.005-0.1:90-100.

[0018] As a preferred technical solution of the present invention, the preparation method of the composite lysozyme concentrate is: lysozyme and lysostaphin are mixed and uniformly mixed, and then added into purified water and stirred to dissolve to obtain the composite lysozyme concentrate.

[0019] As a preferred technical solution of the present invention, the moisturizing agent includes any one of glycerin, propylene glycol and sodium lactate.

[0020] As a preferred technical solution of the present invention, the essence includes but is not limited to any one of camellia essence, rose essence, cherry blossom essence and jasmine essence.

[0021] As a preferred technical solution of the present invention, the preservative includes any one of benzyl alcohol, phenoxyethanol, Nisin (nisin) and ε-polylysine ethyl ester hydrochloride.

[0022] As a preferred technical solution of the present invention, the solubilizer includes any one of polyoxyethylene 40 hydrogenated castor oil, polysorbate 60 and polysorbate 80.

[0023] As a preferred technical solution of the present invention, the pH adjuster includes any one of citric acid, lactic acid and malic acid.

[0024] As a preferred technical solution of the present invention, the type of the gynecological lotion includes any one of gynecological lotion, gynecological mousse, gynecological shower gel and gynecological gel.

[0025] Furthermore, the weight proportions of the raw materials in the gynecological wash are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 1-5 parts by weight of moisturizer, 0.05-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizer, 0.1-0.5 parts by weight of preservative, 0.1-0.5 parts by weight of pH adjuster and 73-92.6 parts by weight of purified water.

[0026] Furthermore, the weight parts of the raw materials in the gynecological mousse are as follows: 5-15 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 1-5 parts by weight of moisturizer, 0.05-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizer, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjuster and 68.45-88.69 parts by weight of purified water.

[0027] Furthermore, the weight proportions of the raw materials in the gynecological shower gel are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 0.1-1 parts by weight of gelling agent, 5-10 parts by weight of moisturizing agent, 0.01-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizing agent, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjusting agent and 67.45-88.64 parts by weight of purified water.

[0028] Furthermore, the weight proportions of the raw materials in the gynecological gel are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 0.5-5 parts by weight of gelling agent, 5-10 parts by weight of moisturizing agent, 0.01-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizing agent, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjusting agent and 63.45-88.24 parts by weight of purified water.

[0029] As a preferred technical solution of the present invention, the preparation method of the gynecological wash and gynecological mousse specifically comprises the following steps: S1. Mix the moisturizer and purified water evenly, add the essence and solubilizer and stir evenly to obtain a mixed solution A; S2, adding the biofilm removal concentrate to the mixed solution A and stirring evenly, adding the composite lysozyme concentrate after stirring evenly, and then adding the recombinant human collagen and stirring to dissolve, to obtain a mixed solution B; S3. Add preservatives to the mixed solution B and stir evenly, adjust the pH of the solution with a pH adjuster, filter and fill to obtain the finished product.

[0030] Furthermore, in step S3, the pH of the solution is adjusted to a pH value of 4.5-5.5.

[0031] As a preferred technical solution of the present invention, the preparation method of the gynecological shower gel and gynecological gel specifically comprises the following steps: S1. Heat purified water, add a gelling agent, mix and stir evenly, and cool to room temperature to obtain a matrix A; S2, adding a moisturizer to the matrix A and mixing evenly, then adding the biofilm removal concentrate and mixing evenly, after the stirring is completed, adding a flavor and a solubilizer and mixing evenly to obtain a matrix B; S3. Add the composite lysozyme concentrate to the matrix B and stir evenly, then add the recombinant human collagen and stir to dissolve. After stirring, add the preservative and stir evenly, adjust the pH of the solution with a pH adjuster, filter and fill to obtain the finished product.

[0032] Furthermore, the heating temperature in step S1 is 60-65° C.; the pH of the solution adjusted in step S3 is pH=4.5-5.5.

[0033] Beneficial effects of the present invention: (1) The present invention selects lysozyme and lysostaphin to form a composite lysozyme concentrate as the antibacterial component of the system. Lysozyme and lysostaphin can work together to bind to negatively charged bacteria and specifically act on the glycosidic bonds and peptide bond bridges of the peptidoglycan in the bacterial cell wall, thereby dissolving the bacterial cell wall, causing the cell to rupture and die, effectively making the finished product have an excellent bactericidal effect.

[0034] (2) The present invention also compounds a nonionic surfactant and a zwitterionic surfactant, preferably alkyl polyglycoside APG0810 and lauryl amide propyl betaine; the two can work together to effectively reduce the surface tension of the biofilm, so that the biofilm formed on the vaginal wall can be disintegrated, thereby achieving the effect of removing the biofilm, which is beneficial to the effective combination of the antibacterial components in the system with bacteria, and further enhancing the bactericidal performance of the finished product.

[0035] (3) The biofilm removal concentrate added in the present invention provides the ability to disintegrate the biofilm, which effectively paves the way for the composite lysozyme concentrate, so that the composite lysozyme concentrate can better combine with bacteria, thereby promoting cell rupture and death; on this basis, recombinant human collagen is also added for care to repair the barrier, and moisturizer is added to increase the wetness to assist in in-depth maintenance. That is, the present invention innovatively combines the biofilm removal concentrate, composite lysozyme concentrate, recombinant human collagen and moisturizer to synergistically play the role of "film removal, sterilization and maintenance", overcoming the shortcomings of female gynecological care products in the prior art, and the product also has the advantages of no drug resistance, reduced recurrence rate, avoidance of adverse side effects, and barrier repair. DETAILED DESCRIPTION

[0036] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0037] The lysozyme used in the embodiments of the present invention was purchased from Dalian Green Snow Egg Products Development Co., Ltd.; lysostaphin was purchased from Shanghai Gaoke Biotechnology Co., Ltd.; and recombinant human collagen was purchased from Guangzhou Xingxing Biotechnology Co., Ltd.; the above will not be repeated in the following.

[0038] Examples 1-5 Preparation of biofilm removal concentrate: uniformly mixing the nonionic surfactant and the zwitterionic surfactant to obtain the biofilm removal concentrate; Preparation of composite lysozyme concentrate: taking lysozyme and lysostaphin, mixing them evenly, adding them into purified water, stirring and dissolving them, to obtain composite lysozyme concentrate; The specific ingredients of each component in Examples 1-5 are shown in Table 1: Table 1

[0039] The weight (g) of each raw material in Examples 1-5 is shown in Table 2: Table 2

[0040] Comparative Example 1 Compared with Example 1, in Comparative Example 1, lysozyme was replaced by lysostaphin, that is, the total mass of the composite lysozyme concentrate remained unchanged, but lysozyme was not added, and the other operating steps and parameters remained unchanged.

[0041] Comparative Example 2 Compared with Example 1, in Comparative Example 2, lysostaphin was replaced by lysozyme, that is, the total mass of the composite lysozyme concentrate remained unchanged, but lysostaphin was not added, and the other operating steps and parameters remained unchanged.

[0042] Comparative Example 3 Compared with Example 1, in Comparative Example 3, the alkyl polyglycoside APG0810 is replaced by lauroyl propyl betaine, that is, the total mass of the biofilm removal concentrate remains unchanged, but the alkyl polyglycoside APG0810 is not added, and the other operating steps and parameters remain unchanged.

[0043] Comparative Example 4 Compared with Example 1, in Comparative Example 4, lauroylamidopropyl betaine was replaced with alkyl glycoside APG0810, that is, the total mass of the biofilm removal concentrate remained unchanged, but lauroylamidopropyl betaine was not added, and the other operating steps and parameters remained unchanged.

[0044] Embodiment 6-8 A method for preparing a bactericidal gynecological wash and gynecological mousse containing composite lysozyme, specifically comprising the following steps: S1. Mix the moisturizer and purified water evenly, add the essence and solubilizer and stir evenly to obtain a mixed solution A; S2, adding the biofilm removal concentrate to the mixed solution A and stirring evenly, adding the composite lysozyme concentrate after stirring evenly, and then adding the recombinant human collagen and stirring to dissolve, to obtain a mixed solution B; S3. Add preservatives to the mixed solution B and stir evenly, use a pH adjuster to adjust the solution pH to 5.0, filter and fill to obtain the finished product.

[0045] A method for preparing a bactericidal gynecological shower gel and gynecological gel containing composite lysozyme, specifically comprising the following steps: S1. Heat purified water to 62.5°C, add a gelling agent, mix and stir evenly, and cool to room temperature to obtain matrix A; S2, adding a moisturizer to the matrix A and mixing evenly, then adding the biofilm removal concentrate and mixing evenly, after the stirring is completed, adding a flavor and a solubilizer and mixing evenly to obtain a matrix B; S3. Add the composite lysozyme concentrate to the matrix B and stir evenly, then add the recombinant human collagen and stir to dissolve. After stirring, add the preservative and stir evenly. Use a pH adjuster to adjust the solution pH to 5.0, filter and fill to obtain the finished product.

[0046] The specific ingredients of each component in Example 6 are shown in Table 3: Table 3

[0047] The weight parts of each component in Example 6 are shown in Table 4: Table 4

[0048] The specific ingredients of each component in Example 7 are shown in Table 5: Table 5

[0049] The weight parts of each component in Example 7 are shown in Table 6: Table 6

[0050] The specific ingredients of each component in Example 8 are shown in Table 7: Table 7

[0051] The weight parts of each component in Example 8 are shown in Table 8: Table 8

[0052] Comparative Example 5 Compared with Example 8, the difference is that the gynecological wash, gynecological mousse, gynecological shower gel and gynecological gel in Comparative Example 5 are all replaced with the corresponding composite lysozyme concentrate prepared in Comparative Example 1, and the other operating steps and parameters remain unchanged.

[0053] Comparative Example 6 Compared with Example 8, the difference is that the gynecological wash, gynecological mousse, gynecological shower gel and gynecological gel in Comparative Example 6 are all replaced with the composite lysozyme concentrate prepared in Comparative Example 2, and the other operating steps and parameters remain unchanged.

[0054] Comparative Example 7 Compared with Example 8, the difference is that the gynecological wash, gynecological mousse, gynecological shower gel and gynecological gel in Comparative Example 7 are all replaced with the biofilm removal concentrate prepared in Comparative Example 3, and the other operating steps and parameters remain unchanged.

[0055] Comparative Example 8 Compared with Example 8, the difference is that the gynecological wash, gynecological mousse, gynecological shower gel and gynecological gel in Comparative Example 8 are all replaced with the biofilm removal concentrate prepared in Comparative Example 4, and the other operating steps and parameters remain unchanged.

[0056] Comparative Example 9 The total weight of the biofilm removal concentrate, the composite lysozyme concentrate, the recombinant human collagen and the moisturizer in Example 8 is named A. Compared with Example 8, the difference of Comparative Example 9 is that, in the gynecological wash, gynecological mousse, gynecological shower gel and gynecological gel in Comparative Example 9, while keeping the total weight of A unchanged, no biofilm removal concentrate is added, and the weight of the composite lysozyme concentrate, the recombinant human collagen and the moisturizer are increased, and the increased weight is 1 / 3 of the composite lysozyme concentrate; the other operating steps and parameters remain unchanged, and the specific weight of each component is shown in Table 9: Table 9

[0057] Comparative Examples 10-12 Taking Comparative Example 9 as an example, Comparative Examples 10-12 are similar. Under the premise of keeping the total weight of parts A unchanged, the composite lysozyme concentrate, recombinant human collagen and moisturizer are not added in turn and the weight parts not added are evenly divided among other raw materials; the other operating steps and parameters remain unchanged.

[0058] Test Example 1 Antibacterial effect test: Referring to the antibacterial test in the "Technical Specifications for Disinfection", antibacterial tests were performed on Examples 6-8 and Comparative Examples 5-12, and the results are shown in Table 10: Table 10

[0059] As can be seen from Table 10, the finished product prepared by the present invention has an excellent bactericidal effect.

[0060] Test Example 2 According to GB 15979-2002 "Sanitary Standards for Disposable Sanitary Products" "Appendix B Product Microbiological Detection Method", Examples 6-8 and Comparative Examples 5-12 were subjected to microbial limit inspection, wherein the standard values ​​of microbial limit inspection are shown in Table 11; the final inspection results are shown in Table 12; Table 11

[0061] Table 12

[0062] As can be seen from Table 12, the finished product prepared by the present invention has an excellent bactericidal effect.

[0063] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0064] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A bactericidal gynecological lotion containing composite lysozyme, characterized in that: The gynecological lotion comprises the following raw materials: Biofilm removal concentrate, compound lysozyme concentrate, recombinant human collagen, moisturizer, flavor, solubilizer, preservative, pH adjuster and purified water; The biofilm removal concentrate comprises a nonionic surfactant and a zwitterionic surfactant in a mass ratio of 1:1-9; The nonionic surfactant includes at least one of alkyl glycosides, fatty alcohol polyoxyethylene ethers, alkylphenol polyoxyethylene ethers and sorbitan fatty acid esters; the zwitterionic surfactant includes cocamidopropyl betaine and lauroamidopropyl betaine; The composite lysozyme concentrate comprises lysozyme, lysostaphin and purified water in a mass ratio of 5-20:0.005-0.1:90-100.

2. The bactericidal gynecological lotion containing composite lysozyme according to claim 1, characterized in that: The gynecological lotion also includes a gelling agent; the gelling agent includes any one or two of hydroxypropyl methylcellulose, hydroxyethyl cellulose, polyvinyl pyrrolidone and poloxamer.

3. The bactericidal gynecological lotion containing composite lysozyme according to claim 1, characterized in that: The alkyl glycosides include alkyl glycoside APG0810 and alkyl glycoside 1214; the fatty alcohol polyoxyethylene ethers include laureth-4 and laureth-23; the alkylphenol polyoxyethylene ethers include nonylphenol polyoxyethylene ether; and the sorbitan fatty acid ester includes PEG-80.

4. The bactericidal gynecological lotion containing composite lysozyme according to claim 1, characterized in that: The moisturizer includes any one of glycerin, propylene glycol and sodium lactate; the flavor includes any one of camellia flavor, rose flavor, cherry flavor and jasmine flavor; the preservative includes any one of benzyl alcohol, phenoxyethanol, nisin and ε-polylysine ethyl ester hydrochloride; the solubilizer includes any one of polyoxyethylene 40 hydrogenated castor oil, polysorbate 60 and polysorbate 80; the pH adjuster includes any one of citric acid, lactic acid and malic acid.

5. The bactericidal gynecological lotion containing composite lysozyme according to claim 1 or 2, characterized in that: The types of the gynecological lotion include any one of a gynecological lotion, a gynecological mousse, a gynecological shower gel and a gynecological gel.

6. The bactericidal gynecological lotion containing composite lysozyme according to claim 5, characterized in that: The weight parts of each raw material in the gynecological lotion are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 1-5 parts by weight of moisturizer, 0.05-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizer, 0.1-0.5 parts by weight of preservative, 0.1-0.5 parts by weight of pH adjuster and 73-92.6 parts by weight of purified water; The weight parts of the raw materials in the gynecological mousse are as follows: 5-15 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 1-5 parts by weight of moisturizer, 0.05-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizer, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjuster and 68.45-88.69 parts by weight of purified water; The weight parts of each raw material in the gynecological shower gel are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 0.1-1 parts by weight of gelling agent, 5-10 parts by weight of moisturizing agent, 0.01-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizing agent, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjusting agent and 67.45-88.64 parts by weight of purified water; The weight parts of the raw materials in the gynecological gel are as follows: 1-10 parts by weight of biofilm removal concentrate, 5-10 parts by weight of composite lysozyme concentrate, 0.05-0.3 parts by weight of recombinant human collagen, 0.5-5 parts by weight of gelling agent, 5-10 parts by weight of moisturizing agent, 0.01-0.2 parts by weight of flavor, 0.1-0.5 parts by weight of solubilizing agent, 0.1-0.5 parts by weight of preservative, 0.005-0.05 parts by weight of pH adjusting agent and 63.45-88.24 parts by weight of purified water.

7. The bactericidal gynecological lotion containing composite lysozyme according to claim 6, characterized in that: The preparation method of the gynecological wash and gynecological mousse specifically comprises the following steps: S1. Mix the moisturizer and purified water evenly, add the essence and solubilizer and stir evenly to obtain a mixed solution A; S2, adding the biofilm removal concentrate to the mixed solution A and stirring evenly, adding the composite lysozyme concentrate after stirring evenly, and then adding the recombinant human collagen and stirring to dissolve, to obtain a mixed solution B; S3, adding preservatives to the mixed solution B and stirring evenly, adjusting the pH of the solution with a pH adjuster, filtering, and filling to obtain a finished product; The preparation method of the gynecological shower gel and gynecological gel specifically comprises the following steps: S1. Heat purified water, add a gelling agent, mix and stir evenly, and cool to room temperature to obtain a matrix A; S2, adding a moisturizer to the matrix A and mixing evenly, then adding the biofilm removal concentrate and mixing evenly, after the stirring is completed, adding a flavor and a solubilizer and mixing evenly to obtain a matrix B; S3. Add the composite lysozyme concentrate to the matrix B and stir evenly, then add the recombinant human collagen and stir to dissolve. After stirring, add the preservative and stir evenly, adjust the pH of the solution with a pH adjuster, filter and fill to obtain the finished product.

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