A multi-environmental regulation in-situ real-time photosynthetic phenotyping imaging system

Abstract

The present application relates to a kind of multi-environment regulation in situ real-time photosynthetic phenotyping system applied to the field of agricultural plant photosynthetic phenotyping, including environmental control system, photoelectric control system, image acquisition system, plant light system, computer, touch screen and plant sample cultivated in detection box, it is box type multi-environment regulation in situ real-time photosynthetic phenotyping system, realize the automatic regulation of experimental environmental factors, and in the experiment process, realize the in situ real-time monitoring and imaging of plant chlorophyll fluorescence parameters in box, improve the accuracy of chlorophyll fluorescence parameter detection, improve the efficiency of research work, and cooperate with adjusting band to realize the parallel of the detection angle of fluorescence imaging CCD camera and the leaf to be detected, guarantee the consistency of detection distance, cooperate with flattening unit, realize the flattening processing of warping part, guarantee the accuracy of detection result.

Description

Technical Field

[0001] This invention relates to an in-situ real-time photosynthetic phenotypic imaging system, and more particularly to a multi-environment-controlled in-situ real-time photosynthetic phenotypic imaging system for use in the field of photosynthetic phenotypic imaging of crop plants. Background Technology

[0002] Photosynthesis is the foundation of crop physiological metabolism and a decisive factor in yield and quality formation. The collection and analysis of photosynthetic efficiency information has become an important part of plant phenomics, which will bring about significant changes to the breeding of stress-resistant and high-yielding varieties and the intelligent regulation of greenhouse growth environment. Among them, chlorophyll fluorescence detection of photosynthetic efficiency has the advantages of simple operation, non-destructive and rapid operation, and has become a routine means to reflect the physiological state and photosynthetic efficiency of plants in plant stress physiology research.

[0003] Chinese invention patent CN202110475367.9 discloses a device and method for detecting chlorophyll fluorescence parameters in plant leaves. Based on the acquired radiance information, fluorescence spectrum and leaf temperature of plant leaves, the chlorophyll fluorescence parameters are calculated to achieve real-time, non-destructive, efficient and accurate detection of chlorophyll fluorescence parameters in plant leaves.

[0004] Existing plant chlorophyll fluorescence detection devices operate by removing leaves from the plant before detection. This results in a difference between the light environment during detection and the light environment during plant growth. Furthermore, the detection area of ​​these devices is limited, making it impossible to achieve overall imaging of larger plants or plant groups. This further limits the detection of photosynthetic efficiency of plant groups. Summary of the Invention

[0005] In view of the above-mentioned prior art, the technical problem to be solved by the present invention is to provide a multi-environmental precise control system that can perform high-throughput, large-area chlorophyll fluorescence imaging, so as to realize in-situ detection of plant experiments or simulate the experimental environment of the plant during the detection process.

[0006] To address the aforementioned issues, this invention provides a multi-environment-controlled in-situ real-time photosynthetic phenotypic imaging system, comprising an environmental control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen, and plant samples cultivated in a detection chamber. The environmental control system is used to control the temperature, humidity, CO2 concentration, and light index of the detection chamber.

[0007] The plant lighting system includes an LED light panel controlled by PWM, which is installed on the inner top wall of the detection box to provide plant growth light, measurement light, photochemical light and saturation pulse light for the plant samples;

[0008] The image acquisition system comprises a fluorescence imaging CCD camera for shooting the chlorophyll fluorescence imaging of the plant sample in the detection box, and transmitting the image data to the computer, the inside of the detection box is divided into a separated chamber and a test chamber by a partition plate, the plant sample is located in the test chamber, a stepper motor is installed in the separated chamber, the output end of the stepper motor is connected with a lead screw penetrating into the test chamber, a moving sleeve is threadedly connected on the surface of the lead screw, one end of the moving sleeve away from the stepper motor is connected with a rectangular block, a control motor is arranged at the bottom of the moving sleeve, the output end of the control motor is connected with an electric extension rod, the power end of the electric extension rod is connected with a frame, a rotating rod is rotatably connected in the frame, a drive motor is installed on the surface of the frame, and the output end of the drive motor is connected with one end of the rotating rod, an adjusting belt is wound on the surface of the rotating rod, the end of the adjusting belt is connected with the fluorescence imaging CCD camera, a smoothing unit is installed at the bottom of the frame, the smoothing unit comprises a servo motor embeddedly installed at the middle position of the bottom of the frame, the output end of the servo motor is connected with a central air pipe through a short shaft, the two ends of the central air pipe are connected with elastic air pipes, magnetic sheet plates are installed on the end surfaces of the two elastic air pipes, and an electromagnetic ring is sleeved on the outside surface of the servo motor.

[0009] The photoelectric control system is used for controlling the plant light system and the environment control system, and the touch screen is installed on the front surface of the detection box.

[0010] In the above multi-environment regulation in-situ real-time photosynthetic phenotype imaging system, the automatic regulation of the test environmental factors is realized, the in-situ real-time detection and imaging of the chlorophyll fluorescence parameters of the plant are realized, and the intelligent data analysis and image processing are simultaneously performed, so that the accuracy of the chlorophyll fluorescence parameter detection is improved, and the research efficiency is improved.

[0011] As a further improvement of the present application, the plant growth light is composed of red light with a wavelength range of 660±5nm, blue light with a wavelength range of 450±5nm and infrared light with a wavelength range of 730±5nm, which is used to provide the plant growth light requirement, the intensity of the measuring light is 1µmolm -2 s -1 , the intensity of the actinic light is 400-600µmolm -2 s -1 , the light energy density of the saturating pulse light is 5000-8000µmolm -2 s -1 , and the saturating pulse light is realized by laser and homogenizer when working.

[0012] As a further improvement of the present application, the actinic light is realized by modulated pulse, and the opening time is 0.2-1.5 seconds.

[0013] As a further improvement of the application, the inside of the partition is provided with a rectangular slot matched with the rectangular block, the cross-sectional height of the rectangular slot is not less than the sum of the length of the moving sleeve, the frame and the electric telescopic rod when not activated, and the rectangular block penetrates the surface of the lead screw.

[0014] As a further improvement of the application, the inside of the adjusting belt is filled with electrorheological fluid, the bottom of the fluorescent imaging CCD camera is provided with a ranging sensor, and the adjusting belt and the driving motor are both connected with the laser ranging sensor limit signal.

[0015] As a further improvement of the application, the surface of the rotating rod is provided with a tooth pattern, and the surface of the adjusting belt close to the rotating rod is provided with a friction pattern matched with the tooth pattern.

[0016] As another improvement of the application, the working steps of the smoothing unit are as follows:

[0017] S1, first determine the position of the leaf to be detected on the surface of the plant sample, and then move the frame down to the top of the leaf to be detected under the cooperation of the electric telescopic rod;

[0018] S2, adjust the fluorescent imaging CCD camera to be parallel to the leaf to be detected by the adjusting belt and the driving motor;

[0019] S3, use the fluorescent imaging CCD camera to take an image of the surface of the leaf to be detected, detect whether the edge of the leaf to be detected is warped, and if it is determined that the warping phenomenon occurs;

[0020] S4, if it is determined that the edge warping phenomenon occurs, determine the warping position by the image of the leaf to be detected taken by the fluorescent imaging CCD camera, start the smoothing unit, adjust the smoothing direction by the servo motor and the electromagnetic ring, and then the fluorescent imaging CCD camera switches the working state to obtain the fluorescent imaging data of the leaf to be detected.

[0021] As a supplement to another improvement of the application, the elastic air pipe is made of elastic and telescopic material, the central air pipe is made of rigid material, and the surfaces of the elastic air pipe and the central air pipe are both provided with air outlets.

[0022] As a supplement to another improvement of the application, the horizontal distance between the bottom of the frame and the adjusting belt is not less than the horizontal length of the central air pipe, and the top end of the magnetic sheet plate is in contact with the bottom of the frame.

[0023] In summary, the beneficial effects of the application are as follows:

[0024] 1. The test processing environment is consistent with the detection environment. When performing chlorophyll fluorescence imaging detection, the light (light quality, light intensity and illumination time), temperature, humidity and carbon dioxide concentration within a certain range are precisely controlled to ensure the accuracy of photosynthetic phenotype detection under multiple environmental factors of plants;

[0025] 2. Through intelligent control, the chlorophyll fluorescence detection process can be automatically darkened, and the accuracy of F0 and other fluorescence chlorophyll fluorescence parameters can be improved. At the same time, intelligent control makes it possible to study the relationship between variable light (light quality, light intensity) fluorescence induction curve analysis, plant growth and development stage and rhythm and chlorophyll fluorescence, which can promote the development of chlorophyll fluorescence analysis technology and the development of different application research fields;

[0026] 3. The plant test growth light, measurement light and actinic light share one LED light plate, which realizes the combination of different functions through PWM control, ensures the consistency of light quality, light intensity and other light environments during plant testing and measurement, and reduces the hardware investment cost;

[0027] 4. The test equipment and detection instrument are unified, and plant testing and measurement can be performed simultaneously to realize in-situ detection without moving, reduce damage to test samples, and improve research efficiency.

[0028] 5. The adjustment belt realizes the parallelism of the fluorescence imaging CCD camera detection angle and the leaf to be detected, ensures the consistency of the detection distance, and cooperates with the flattening unit to realize the flattening of the warped part, ensuring the accuracy of the detection result. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 The system composition diagram of the first embodiment of the present application;

[0030] Figure 2 The detection box external structure diagram of the first embodiment of the present application;

[0031] Figure 3 The detection box internal structure diagram of the first embodiment of the present application;

[0032] Figure 4 The moving sleeve surface structure installation diagram of the first embodiment of the present application;

[0033] Figure 5 The adjustment belt adjusts the fluorescence imaging CCD camera angle diagram of the first embodiment of the present application;

[0034] Figure 6 The tooth pattern and friction pattern diagram of the first embodiment of the present application;

[0035] Figure 7Figure 2 shows a leaf edge warping state diagram for the plant sample of the second embodiment of the present application;

[0036] Figure 8 Figure 5 shows a schematic diagram of the installation of the centering air pipe and the elastic air pipe and the magnetic sheet plate of the second embodiment of the present application;

[0037] Figure 9 Figure 6 shows a simultaneous air exhaust smoothing state diagram for the centering air pipe and the elastic air pipe of the second embodiment of the present application;

[0038] Figure 10 Figure 7 shows a state diagram for the elastic air pipe contraction and the centering air pipe turning for air exhaust smoothing of the second embodiment of the present application.

[0039] Figure legend:

[0040] 1, detection box; 2, LED light plate; 3, stepper motor; 4, lead screw; 5, electric telescopic rod; 6, drive motor; 7, adjusting belt; 8, fluorescence imaging CCD camera; 9, control motor; 10, servo motor; 11, magnetic sheet plate; 12, elastic air pipe; 13, centering air pipe. DETAILED DESCRIPTION

[0041] The two embodiments of the present application will be described in detail below with reference to the accompanying drawings.

[0042] First embodiment:

[0043] Figure 1 A multi-environment control in-situ real-time photosynthetic phenotype imaging system is shown, which comprises an environment control system, a photoelectric control system, an image acquisition system, a plant light system, a computer, a touch screen and a plant sample cultivated in a detection box 1, wherein the environment control system is used to control the temperature, humidity, CO2 concentration and light index of the detection box 1;

[0044] The plant light system comprises a PWM-controlled LED light plate 2, and the LED light plate 2 is installed on the inner top wall of the detection box 1 to provide plant growth light, measurement light, actinic light and saturation pulse light for the plant sample;

[0045] Figures 2-4It is shown that the image acquisition system comprises a fluorescence imaging CCD camera 8 for shooting the chlorophyll fluorescence imaging of the plant sample in the detection box 1 and transmitting the image data to the computer, the inside of the detection box 1 is divided into a separate compartment and a test compartment by a partition, the plant sample is located in the test compartment, a stepper motor 3 is installed in the separate compartment, the output end of the stepper motor 3 is connected with a lead screw 4 penetrating into the test compartment, a moving sleeve is threadedly sleeved on the surface of the lead screw 4, one end of the moving sleeve away from the stepper motor 3 is connected with a rectangular block, a control motor 9 is arranged at the bottom of the moving sleeve, the output end of the control motor 9 is connected with an electric telescopic rod 5, the power end of the electric telescopic rod 5 is connected with a frame, a rotating rod is rotatably connected in the frame, a drive motor 6 is installed on the surface of the frame, and the output end of the drive motor 6 is connected with one end of the rotating rod, and the surface of the rotating rod is wound with an adjusting belt 7, and the end of the adjusting belt 7 is connected with the fluorescence imaging CCD camera 8.

[0046] The photoelectric control system is used for controlling the plant light system and the environment control system, and the touch screen is installed on the front of the detection box 1.

[0047] Specifically, when the multi-environment regulation in-situ real-time photosynthetic phenotype imaging operation is performed, since the plant sample is planted in the inside of the detection box 1, in-situ detection can be realized, the process of taking material-transporting-laboratory-detecting is shortened, the real-time detection effect is ensured, and the detection accuracy is ensured.

[0048] In addition, the environment control system is used for adjusting the temperature, humidity, light intensity and CO2 concentration in the detection box 1, so as to simulate the environment of the normal growth state of the plant, and the touch screen is used for adjusting and controlling the test environmental factor parameters in the detection box 1.

[0049] In order to avoid the influence of the humidity in the test compartment on the high-precision stepper motor 3 and related electrical elements in a non-detection state, the stepper motor 3 can be started to control the moving sleeve to move on the surface of the lead screw 4 into the separate compartment, and in this state, the rectangular block can block the rectangular slot penetrating through the separate compartment and the test compartment, so as to isolate the influence of the humidity in the test compartment.

[0050] During detection, the stepper motor 3 is started to drive the fluorescence imaging CCD camera 8 to move into the test compartment through the rectangular slot, then the electric telescopic rod 5 is started to make the fluorescence imaging CCD camera 8 move downward, and the direction of the fluorescence imaging CCD camera 8 is adjusted by the control motor 9 in cooperation with the orientation of the leaf to be detected, then the fluorescence imaging CCD camera 8 is horizontally hung above the leaf to be detected, since the leaves on the surface of the plant are not horizontally placed during growth (in this embodiment, the cucumber plant is taken as an example), therefore, after the fluorescence imaging CCD camera 8 is horizontally hung, the drive motor 6 can be started to control the rotating rod to rotate, and then the adjusting belt 7 adjusts the length of the two ends, so as to adjust the angle of the fluorescence imaging CCD camera 8 (for example, the angle of the fluorescence imaging CCD camera 8 is adjusted to 45 degrees in this embodiment).Figure 5 The angle of the fluorescent imaging CCD camera 8 is adjusted to be parallel to the leaf to be detected, so as to ensure the consistency of the detection distance and the reliability of the detection result.

[0051] In addition, the leaf area is large, and the moving sleeve and the fluorescent imaging CCD camera 8 need to be moved under the cooperation of the stepping motor 3 to realize multi-point detection and image splicing after imaging, so that a relatively complete detection image can be obtained.

[0052] The plant growth light is composed of red light with a wavelength range of 660±5nm, blue light with a wavelength range of 450±5nm, and infrared light with a wavelength range of 730±5nm, which is used to provide plant growth light requirements. The intensity of the measuring light is 1µmolm -2 s -1 The intensity of the actinic light is 400-600µmolm -2 s -1 The light energy density of the saturated pulse light is 5000-8000µmolm -2 s -1 The saturated pulse light is realized by laser and homogenizer when working.

[0053] The actinic light is realized by modulated pulse, and the opening time is 0.2-1.5 seconds.

[0054] The inside of the partition is provided with a rectangular groove matched with the rectangular block, the cross-sectional height value of the rectangular groove is not less than the sum of the length values of the moving sleeve, the frame member and the electric extension rod 5 when not started, and the rectangular block penetrates the surface of the lead screw 4.

[0055] Specifically, the rectangular groove is used to pass through the test bin and the split bin, and the rectangular groove corresponding to the rectangular block can drive the moving sleeve and the related components connected to the surface to smoothly pass through the rectangular groove when the electric extension rod 5 is not started, so as to realize the transfer of the object.

[0056] The inside of the adjusting belt 7 is filled with electrorheological fluid, and the bottom of the fluorescent imaging CCD camera 8 is provided with a distance sensor, and the adjusting belt 7 and the driving motor 6 are connected with the laser distance sensor limit signal.

[0057] Specifically, when the angle of the fluorescent imaging CCD camera 8 is adjusted by the adjusting belt 7, the distance values between multiple position points on the surface of the fluorescent imaging CCD camera 8 (i.e. the position points of the distance sensor) and the surface of the leaf to be detected are obtained, and when the distance values of the multiple position points are consistent, the electrorheological fluid in the adjusting belt 7 is electrified, so that the adjusting belt 7 is solidified, and the solidification constraint effect is realized on the fluorescent imaging CCD camera 8, so as to avoid the change of the detection area of the fluorescent imaging CCD camera 8 caused by subsequent shaking, and to ensure the detection accuracy.

[0058] Figure 6 It is shown that the surface of the rotating rod is provided with a tooth pattern, and the surface of the adjusting belt 7 close to the rotating rod is provided with a friction pattern matched with the tooth pattern.

[0059] Specifically, the design of the tooth pattern and the friction pattern can make the rotating rod rotate to synchronously and stably drive the adjusting belt 7 to move stably, thereby realizing the adjusting effect of the end length.

[0060] The second embodiment is as follows:

[0061] Figure 8 It is shown that the bottom of the frame member is provided with a flattening unit, the flattening unit includes a servo motor 10 inlaidly installed at the middle position of the bottom of the frame member, the output end of the servo motor 10 is connected with a center air pipe 13 through a short shaft, both ends of the center air pipe 13 are connected with elastic air pipes 12, the end surfaces of the two elastic air pipes 12 are both provided with magnetic sheet plates 11, and the outer surface of the servo motor 10 is sleeved with an electromagnetic ring.

[0062] The elastic air pipes 12 are made of elastic and flexible materials, the center air pipe 13 is made of rigid materials, and the surfaces of the elastic air pipes 12 and the center air pipe 13 are both provided with air outlets.

[0063] The horizontal distance value between the bottom of the frame member and the adjusting belt 7 is not less than the horizontal length value of the center air pipe 13, and the top end of the magnetic sheet plate 11 is in contact with the bottom of the frame member.

[0064] The working steps of the flattening unit are as follows:

[0065] S1, first determine the position of the to-be-detected leaf on the surface of the plant sample, and then move the frame member to the upper side of the to-be-detected leaf under the cooperation of the electric telescopic rod 5;

[0066] S2, adjust the fluorescent imaging CCD camera 8 to be parallel to the to-be-detected leaf by using the adjusting belt 7 and the driving motor 6;

[0067] S3, use the fluorescent imaging CCD camera 8 to take an image of the surface of the to-be-detected leaf, detect whether the edge of the to-be-detected leaf is warped, and if it is determined that the edge is warped;

[0068] S4, if it is determined that the edge is warped, determine the warped position by using the image of the to-be-detected leaf taken by the fluorescent imaging CCD camera 8, start the flattening unit, adjust the flattening direction by using the servo motor 10 and the electromagnetic ring, and then switch the working state of the fluorescent imaging CCD camera 8 to obtain the fluorescent imaging data of the to-be-detected leaf.

[0069] Different from the first embodiment, Figure 7It is shown that the present embodiment is aimed at the curling of the edge part of the plant leaf under drought and diseased state, and further causes the coincidence of the curled part when the subsequent fluorescence imaging CCD camera 8 detects the fluorescence imaging of the inside of the leaf. In the present embodiment, the surface of the frame member is connected with an air pipe with a valve (which is a prior art, not shown, and the non-high-speed airflow into the air pipe avoids the impact of high-speed airflow which is too strong to cause impact damage to the curled part of the leaf when smoothing).

[0070] Specifically, when the fluorescence imaging CCD camera 8 is lowered, the surface morphology of the leaf to be detected is scanned first, and then when it is determined that there is an edge curling phenomenon on the surface of the leaf to be detected, if the curled part is on both sides of the frame member, the valve on the surface of the air pipe is directly started, and the discharged gas is discharged through the central air pipe 13 and the elastic air pipe 12, which has a smoothing effect on the curled part on both sides of the frame member during the subsequent fluorescence imaging detection process of the fluorescence imaging CCD camera 8 (as shown in Figure 9 When subsequent reset is required, the magnetic force between the electromagnetic ring and the magnetic sheet plate 11 is switched to repulsion to drive the magnetic sheet plate 11 and the elastic air pipe 12 to reset;

[0071] If the curled part is in an asymmetric design, the electromagnetic ring can be started first as needed to generate an attractive force with the magnetic sheet plate 11, so that the magnetic sheet plate 11 is attracted to each other and drives the elastic air pipe 12 to compress, and then the servo motor 10 is started to drive the central air pipe 13 to rotate to the required smoothing angle, and then the valve is opened. At this time, since the elastic air pipe 12 is in a folded and compressed state, the airflow is limited and mainly concentrated in the central air pipe 13, so that the adjustment of the smoothing airflow direction can be realized (as shown in Figure 10 ).

[0072] In summary, the present application is a box-type multi-environmental control in-situ real-time photosynthetic phenotyping imaging system, which realizes the automatic control of the test environmental factors (light, temperature, humidity, CO2 concentration), and realizes the in-situ real-time monitoring and imaging of the chlorophyll fluorescence parameters of the plant inside the box during the experiment, improves the accuracy of the chlorophyll fluorescence parameter detection, improves the efficiency of the research work, and cooperates with the adjusting belt 7 to realize the parallelism of the detection angle of the fluorescence imaging CCD camera 8 and the leaf to be detected, ensures the consistency of the detection distance, cooperates with the smoothing unit to realize the smoothing processing of the curled part, and ensures the accuracy of the detection result.

[0073] In combination with the current actual demand, the above-mentioned embodiments adopted by the present application are not limited to this, various changes made within the knowledge range of those skilled in the art without departing from the concept of the present application still fall within the protection scope of the present application.

Claims

1. A multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system, comprising: The plant light system comprises a LED lamp panel (2) controlled by PWM, and the LED lamp panel (2) is installed on the inner top wall of the detection box (1) to provide plant growth light, measurement light, actinic light and saturation pulse light for the plant sample. The image acquisition system comprises a fluorescence imaging CCD camera (8) for shooting the chlorophyll fluorescence imaging condition of the plant sample in the detection box (1) and transmitting the shot image data to the computer. The photoelectric control system is used for controlling the plant light system and the environment control system, and the touch screen is installed on the front of the detection box (1). The actinic light is realized by modulating pulse, and the opening time is 0.2-1.5 seconds. The inside of the partition is provided with a rectangular groove matched with the rectangular block, the cross-sectional height value of the rectangular groove is not less than the length value of the sum of the moving sleeve, the frame and the electric extension rod (5) when not started, and the rectangular block penetrates through the surface of the lead screw (4).

2. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The plant growth light is composed of red light with a wavelength range of 660±5nm, blue light with a wavelength range of 450±5nm and infrared light with a wavelength range of 730±5nm, and is used to provide plant growth light requirements, the intensity of the measuring light is 1µmolm -2 s -1 , the intensity of the actinic light is 400-600µmolm -2 s -1 , the light energy density of the saturated pulse light is 5000-8000µmolm -2 s -1 , and the saturated pulse light is realized by laser and a homogenizer when working.

3. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The inside of the adjusting belt (7) is filled with electrorheological fluid, the bottom of the fluorescence imaging CCD camera (8) is provided with a distance measuring sensor, and the adjusting belt (7) and the driving motor (6) are connected with the laser distance measuring sensor limit signal.

4. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The photoelectric control system is used for controlling the plant light system and the environment control system, and the touch screen is installed on the front of the detection box (1).

5. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The elastic air pipe (12) is made of elastic and flexible material, the central air pipe (13) is made of rigid material, and the surfaces of the elastic air pipe (12) and the central air pipe (13) are provided with air outlets. The actinic light is realized by modulating pulse, and the opening time is 0.2-1.5 seconds. The inside of the partition is provided with a rectangular groove matched with the rectangular block, the cross-sectional height value of the rectangular groove is not less than the length value of the sum of the moving sleeve, the frame and the electric extension rod (5) when not started, and the rectangular block penetrates through the surface of the lead screw (4). The inside of the adjusting belt (7) is filled with electrorheological fluid, the bottom of the fluorescence imaging CCD camera (8) is provided with a distance measuring sensor, and the adjusting belt (7) and the driving motor (6) are connected with the laser distance measuring sensor limit signal.

6. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The surface of the rotating rod is provided with a tooth pattern, and the adjusting belt (7) is provided with a friction pattern matched with the tooth pattern.

7. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The working steps of the flattening unit are as follows: S1, first determine the position of the leaf to be detected on the surface of the plant sample, then move the frame member to the upper side of the leaf to be detected under the cooperation of the electric stretching rod (5); S2, adjust the fluorescent imaging CCD camera (8) to be parallel to the leaf to be detected by using the adjusting belt (7) and the driving motor (6); S3, use the fluorescent imaging CCD camera (8) to take an image of the surface of the leaf to be detected, and detect whether the edge of the leaf to be detected is warped, if it is determined that the edge is warped; S4, if it is determined that the edge is warped, determine the warped position by using the image of the leaf to be detected taken by the fluorescent imaging CCD camera (8), start the flattening unit, adjust the flattening direction by using the servo motor (10) and the electromagnetic ring, and then switch the working state of the fluorescent imaging CCD camera (8) to obtain the fluorescent imaging data of the leaf to be detected.

8. The multi-environmental controlled in-situ real-time photosynthetic phenotyping imaging system according to claim 1, wherein: The horizontal distance value between the bottom of the frame member and the adjusting belt (7) is not less than the horizontal length value of the central air pipe (13), and the top end of the magnetic sheet plate (11) is in contact with the bottom of the frame member.

Images