Drought-resistant treatment method for corn seeds

Abstract

The invention relates to the technical field of seed treatment, and discloses a corn seed drought resistance treatment method, which specifically comprises: 1, selecting corn seeds, carrying out a germination test, carrying out surface disinfection for 10 min with 0.1% sodium hypochlorite, and washing for a plurality of times with a small amount of distilled water until no sodium hypochlorite smell exists; step 2, randomly dividing the seeds into a plurality of parts, and respectively soaking the seeds in drought-resistant treatment agents with different concentrations; 3, the germination rate, the germination potential and the germination index are measured, the soaked seeds can enhance the drought resistance in the seedling stage, and drought resistance and seedling protection are facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of seed treatment, in particular to a drought resistance treatment method for corn seeds. Background Art

[0002] Corn is rich in carbohydrates and is an important source of energy for the body. For example, after physical labor or exercise, eating corn can quickly replenish the energy consumed. Corn is rich in dietary fiber, which helps promote intestinal peristalsis, prevent constipation and improve digestive function. Just like some people have indigestion caused by long-term irregular diet, eating corn in moderation can help relieve it, and to obtain corn, it is necessary to grow corn.

[0003] For dryland areas, when choosing corn varieties, it is necessary to focus on the regional environmental climate characteristics, market conditions, seasonal changes, and annual precipitation. Try to choose drought-resistant varieties with high hybrid purity, strong stress resistance, developed root system, compact plant type, slender leaves, dense veins, upward leaves, strong resistance to diseases and pests, high yield and quality, strong resistance and tolerance, and a long growth period (more than 4 months). Compared with other regions, the phenomenon of water loss in drylands is more serious. Therefore, in order to ensure the normal growth of corn, corn seeds need to be treated for drought resistance.

[0004] Treating corn seeds before sowing is one of the simplest, most economical and effective methods to improve sowing quality and seedling stress resistance. Seed treatment can break dormancy, promote germination and increase seedling stress resistance. However, current seed treatment technology still cannot meet the needs of modern agricultural development.

[0005] For corn seeds, in the prior art, drought resistance treatment for corn seeds is mostly carried out by soaking in a single treatment agent, and there is a lack of composite agent treatment. Summary of the invention

[0006] In view of the technical problem that, in the prior art, drought resistance treatment of corn seeds is mostly carried out by soaking in a single treatment agent and there is a lack of composite agent treatment, the present invention provides a drought resistance treatment method for corn seeds.

[0007] The technical solution adopted by the present invention is: a method for drought resistance treatment of corn seeds, which specifically comprises the following steps:

[0008] Corn seeds were selected for germination test. After surface disinfection with 0.1% sodium hypochlorite for 10 minutes, they were rinsed several times with small amounts of distilled water until the smell of sodium hypochlorite disappeared.

[0009] The seeds were randomly divided into several portions and soaked in different concentrations of drought-resistant treatment agents;

[0010] Determination of germination rate, germination potential and germination index.

[0011] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight: 1-5 parts by weight of rooting powder, CaCl 2 The invention discloses a novel novel nanostructured carbon foam, which is composed of 1-10 parts by weight, 5-15 parts by weight of polyethylene glycol, 3-8 parts by weight of gibberellin, 2-10 parts by weight of salicylic acid, 1-3 parts by weight of ethephon and 2-5 parts by weight of methylcyclopropene.

[0012] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0013] 1 part by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and is composed of 1 part by weight, 5 parts by weight of polyethylene glycol, 3 parts by weight of gibberellin, 2 parts by weight of salicylic acid, 1 part by weight of ethephon and 2 parts by weight of methylcyclopropene.

[0014] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0015] 2 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam, comprising: 3 parts by weight, 6 parts by weight of polyethylene glycol, 4 parts by weight of gibberellin, 4 parts by weight of salicylic acid, 1.5 parts by weight of ethephon and 2.5 parts by weight of methylcyclopropene.

[0016] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0017] 3 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and the nanostructured carbon foam. The nanostructured carbon foam is composed of 5 parts by weight, 7 parts by weight of polyethylene glycol, 5 parts by weight of gibberellin, 5 parts by weight of salicylic acid, 2 parts by weight of ethephon and 3 parts by weight of methylcyclopropene.

[0018] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0019] 45 parts by weight of rooting powder, CaCl 2 The invention discloses a novel novel nanostructured carbon foam, which is composed of 8 parts by weight, 10 parts by weight of polyethylene glycol, 6 parts by weight of gibberellin, 7 parts by weight of salicylic acid, 2.5 parts by weight of ethephon and 4 parts by weight of methylcyclopropene.

[0020] Furthermore, the drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0021] 5 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and the nanostructured carbon foam. The nanostructured carbon foam is composed of 10 parts by weight, 15 parts by weight of polyethylene glycol, 8 parts by weight of gibberellins, 10 parts by weight of salicylic acid, 3 parts by weight of ethephon and 5 parts by weight of methylcyclopropene.

[0022] Furthermore, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0023] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0024] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0025] Germination index:

[0026]

[0027] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0028] Furthermore, it also includes the measurement of vitality index, wherein:

[0029] GI=GP×S

[0030] Where: S is the average fresh weight of each plant within the specified time.

[0031] The beneficial effects of the present invention are as follows: compared with the prior art, in the present invention, the investigation of the germination potential, germination rate and germination index of corn seeds shows that the corn seeds themselves have a certain drought resistance, which provides basic materials for corn drought-resistant breeding; therefore, it is necessary to select appropriate drought-resistant measures. In Examples 1 to 5, five different drought-resistant treatment agents can significantly improve the drought resistance of corn seeds by soaking them, and the effect in Example 2 is the best. In addition, root length means a strong water absorption capacity. Appropriate water stress can promote the growth of the radicle and hypocotyl of corn seeds. High-intensity water stress inhibits growth, and the effect of hypocotyl inhibition is more obvious. In Examples 1 to 5, five different drought-resistant treatment agents are used, among which the seed soaking effect in Example 2 is the best. Water stress reduces the germination rate of corn seeds, hinders the growth of radicles and plumules, and the accumulation of dry matter. However, the corn seeds treated with the drought-resistant agents in Examples 1 to 5 are more drought-resistant than those in the control group. There are obvious differences compared with the results; under water stress, the seeds soaked with the drought-resistant agent in Examples 1 to 5 still maintain a high germination rate, reducing the damage of drought stress to the radicle and plumule of the seeds; it can be seen that the composite agent composed of 1 weight part of drought-resistant treatment agent rooting powder, 1 weight part of CaCl2, 5 weight parts of polyethylene glycol, 3 weight parts of gibberellin, 2 weight parts of salicylic acid, 1 weight part of ethephon and 2 weight parts of methylcyclopropene has the best effect; the soaked seeds can enhance the drought resistance in the seedling stage, which is beneficial to drought resistance and seedling protection. DETAILED DESCRIPTION

[0032] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] In order to solve the problems existing in the background technology, the present application proposes the following technical solution: a method for drought resistance treatment of corn seeds, specifically comprising the following steps:

[0034] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0035] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0036] Step three: Determination of germination rate, germination potential and germination index.

[0037] In further design:

[0038] The drought-resistant treatment agent is composed of the following raw materials in parts by weight: 1-5 parts by weight of rooting powder, CaCl 2 The invention discloses a novel novel nanostructured carbon foam, which is composed of 1-10 parts by weight, 5-15 parts by weight of polyethylene glycol, 3-8 parts by weight of gibberellin, 2-10 parts by weight of salicylic acid, 1-3 parts by weight of ethephon and 2-5 parts by weight of methylcyclopropene.

[0039] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0040] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0041] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0042] Germination index:

[0043]

[0044] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0045] It also includes a measure of vitality, where:

[0046] GI (vitality index) = GP × S

[0047] Where: S is the average fresh weight of each plant within the specified time.

[0048] Embodiment 1

[0049] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0050] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0051] Step three: Determination of germination rate, germination potential and germination index.

[0052] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0053] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0054] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0055] Germination index:

[0056]

[0057] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0058] It also includes a measure of vitality, where:

[0059] GI (vitality index) = GP × S

[0060] Where: S is the average fresh weight of each plant within the specified time.

[0061] The drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0062] 1 part by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and is composed of 1 part by weight, 5 parts by weight of polyethylene glycol, 3 parts by weight of gibberellin, 2 parts by weight of salicylic acid, 1 part by weight of ethephon and 2 parts by weight of methylcyclopropene.

[0063] Embodiment 2

[0064] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0065] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0066] Step three: Determination of germination rate, germination potential and germination index.

[0067] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0068] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0069] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0070] Germination index:

[0071]

[0072] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0073] It also includes a measure of vitality, where:

[0074] GI (vitality index) = GP × S

[0075] Where: S is the average fresh weight of each plant within the specified time.

[0076] The drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0077] 2 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam, comprising: 3 parts by weight, 6 parts by weight of polyethylene glycol, 4 parts by weight of gibberellin, 4 parts by weight of salicylic acid, 1.5 parts by weight of ethephon and 2.5 parts by weight of methylcyclopropene.

[0078] Embodiment 3

[0079] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0080] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0081] Step three: Determination of germination rate, germination potential and germination index.

[0082] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0083] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0084] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0085] Germination index:

[0086]

[0087] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0088] It also includes a measure of vitality, where:

[0089] GI (vitality index) = GP × S

[0090] Where: S is the average fresh weight of each plant within the specified time.

[0091] The drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0092] 3 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and the nanostructured carbon foam. The nanostructured carbon foam is composed of 5 parts by weight, 7 parts by weight of polyethylene glycol, 5 parts by weight of gibberellin, 5 parts by weight of salicylic acid, 2 parts by weight of ethephon and 3 parts by weight of methylcyclopropene.

[0093] Embodiment 4

[0094] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0095] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0096] Step three: Determination of germination rate, germination potential and germination index.

[0097] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0098] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0099] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0100] Germination index:

[0101]

[0102] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0103] It also includes a measure of vitality, where:

[0104] GI (vitality index) = GP × S

[0105] Where: S is the average fresh weight of each plant within the specified time.

[0106] The drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0107] 45 parts by weight of rooting powder, CaCl 2 The invention discloses a novel novel nanostructured carbon foam, which is composed of 8 parts by weight, 10 parts by weight of polyethylene glycol, 6 parts by weight of gibberellin, 7 parts by weight of salicylic acid, 2.5 parts by weight of ethephon and 4 parts by weight of methylcyclopropene.

[0108] Embodiment 5

[0109] Step 1: Select corn seeds for germination test, disinfect the surface with 0.1% sodium hypochlorite for 10 minutes, and then rinse with distilled water several times in small amounts until there is no smell of sodium hypochlorite;

[0110] Step 2: randomly divide the seeds into several portions and soak them in drought-resistant treatment agents of different concentrations;

[0111] Step three: Determination of germination rate, germination potential and germination index.

[0112] Among them, the calculation formulas for germination rate, germination potential and germination index are as follows:

[0113] Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested;

[0114] Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time;

[0115] Germination index:

[0116]

[0117] Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

[0118] It also includes a measure of vitality, where:

[0119] GI (vitality index) = GP × S

[0120] Where: S is the average fresh weight of each plant within the specified time.

[0121] The drought-resistant treatment agent is composed of the following raw materials in parts by weight:

[0122] 5 parts by weight of rooting powder, CaCl 2 The invention relates to a novel nanostructured carbon foam and the nanostructured carbon foam. The nanostructured carbon foam is composed of 10 parts by weight, 15 parts by weight of polyethylene glycol, 8 parts by weight of gibberellins, 10 parts by weight of salicylic acid, 3 parts by weight of ethephon and 5 parts by weight of methylcyclopropene.

[0123] Comparative Example

[0124] Soak in distilled water.

[0125] Experimental example

[0126] From the same batch, five portions of corn seeds, each with fifty seeds, were selected and respectively placed in the treatment solutions of Examples 1 to 5 for 8-10 hours;

[0127] Then, the seeds were placed in a culture dish, 50 seeds per dish, and 20 mL of distilled water and 15% PEG-6000 (w / v) were added as the infiltration medium, forming a water potential of -0.6 Mpa. The control was soaked in clear water.

[0128] Experimental land was selected and divided into six parts (A, B, C, D, E, and F), corresponding to Examples 1 to 5 and the comparative example, respectively, and the soaked seeds were planted in the corresponding land, and the corn seeds were cultivated to five leaves;

[0129] The water retention capacity of detached leaves was measured by soaking them in 10% polyethylene glycol (PEG-6000) to simulate drought stress for 36 hours; one,

[0132] Measurement indicators

[0133] The germination rate was determined from the 2nd to the 6th day, and the plumule length, radicle length and radicle dry weight were measured within 16 days.

[0134] Seed germination drought resistance index (GDRI) = seed germination stress index (PIs) / control seed germination index (PIc);

[0135] Where: PI = (1.00) × nd 2 +(0.75)×nd 4 +(0.50)×nd 6

[0136] Where: nd 2 ,nd 4 ,nd 6 The seed germination rates on the 2nd, 4th, and 6th days, respectively;

[0137] Ten days after observation, the seedlings were taken out and their height and root length were measured with a ruler (mm);

[0138] The water retention capacity of detached leaves was determined by weighing method. Three leaves were taken from plants with sufficient water supply and placed in a room. The leaves were weighed at regular intervals to calculate the water content of the detached leaves (as a percentage of the natural fresh weight).

[0139] Leaf water content (%) = (w fn -w d ) / w f ×100

[0140] Where: w f w is the natural fresh weight of leaves; d w is the dry weight of leaves; fn is the weight of the leaf at each time period.

[0141] Table 1 Effects of the solutions in Examples 1 to 5 and Comparative Examples on the germination characteristics of corn seeds

[0142]

[0143] Table 1

[0144] To sum up:

[0145] As can be seen from Table 1, under osmotic stress, the corn seeds treated with different drought-resistant agents (Examples 1 to 5 and Comparative Example) had different final germination rates after being treated at a water potential of -0.6 MPa for 6 days, and there were certain differences in the germination process. The investigation of the germination potential, germination rate and germination index of corn seeds showed that the corn seeds themselves had certain drought resistance, which provided basic materials for drought-resistant corn breeding; therefore, it was necessary to select appropriate drought-resistant measures. In Examples 1 to 5, five different drought-resistant treatment agents were used to significantly improve the drought resistance of corn seeds by soaking the seeds.

[0146] The investigation of the germination potential, germination rate and germination index of corn seeds shows that corn seeds themselves have a certain drought resistance, which provides a basic material for drought-resistant corn breeding; therefore, it is necessary to select appropriate drought-resistant measures. In Examples 1 to 5, five different drought-resistant treatment agents can significantly improve the drought resistance of corn seeds by soaking seeds, and the effect in Example 2 is the best.

[0147] The germination rates of seeds soaked in Example 1 were the highest, and the germination rates of seeds soaked in wood vinegar in Examples 2 to 5 were also higher than those in the comparative example;

[0148] It can be seen that the corn seeds treated with drought-resistant agents have advantages over the control group.

[0149] Table 2 Effects of the solutions in Examples 1 to 5 and Comparative Examples on the growth of corn seedlings

[0150] Example Seedling height (mm) Root length(mm) Embodiment 1 100.48±10.28 20.12±1.01 Embodiment 2 190.58±10.67 2.63±1.12 Embodiment 3 140.53±10.18 20.12±1.11 Embodiment 4 110.40±10.37 10.48±1.12 Embodiment 5 180.80±10.23 20.07±1.11 Comparative Example 10.80±10.23 10.11±1.05

[0151] Table 2

[0152] As can be seen from Table 2, the corn seeds soaked in Example 1 to Example 5 have a radicle length, a plumule length, and a radicle dry weight that are higher than the comparative example. The radicle length of the corn seeds treated with drought-resistant agents in Example 1 to Example 5 is larger than that of the comparative example under normal conditions, and the effect of Example 5 is the most obvious. The above results show that the corn seeds soaked in Example 1 to Example 5 have the effect of promoting the growth of radicle and plumule and the accumulation of radicle dry matter, showing that seed germination has a certain drought resistance, and to a certain extent reduces the adverse effects of drought stress on corn emergence. Root length means that there is a strong water absorption capacity, and appropriate water stress can promote the growth of corn seed radicle and hypocotyl, and high-intensity water stress inhibits growth, and the effect of hypocotyl inhibition is more obvious. In Example 1 to Example 5, five different drought-resistant treatment agents, wherein the seed soaking effect in Example 2 is the best.

[0153] Table 3 Changes in water content of detached leaves of corn in each period of time treated in Example 1 to Example 5

[0154]

[0155] Table 3

[0156] It can be seen from Table 3 that the greater the water loss, the worse the water retention effect, so the effect in Example 2 is the best. Water stress reduces the germination rate of corn seeds, hinders the growth of radicles and plumules and the accumulation of dry matter, but the corn seeds treated with drought-resistant agents in Examples 1 to 5 have obvious differences in drought resistance compared with the comparative example; under water stress, the seeds soaked with drought-resistant agents in Examples 1 to 5 still maintain a high germination rate, reducing the damage of drought stress to the radicles and plumules of seeds; it can be seen that the drought-resistant treatment agent rooting powder 1 part by weight, CaCl 2 The best effect is achieved by a composition of 1 part by weight of sodium pyrochloreate, 5 parts by weight of polyethylene glycol, 3 parts by weight of gibberellin, 2 parts by weight of salicylic acid, 1 part by weight of ethephon and 2 parts by weight of methylcyclopropene; the soaked seeds can enhance the drought resistance in the seedling stage.

[0157] In summary

[0158] The investigation of the germination potential, germination rate and germination index of corn seeds shows that corn seeds themselves have a certain drought resistance, which provides a basic material for drought-resistant corn breeding; therefore, it is necessary to select appropriate drought-resistant measures. In Examples 1 to 5, five different drought-resistant treatment agents can significantly improve the drought resistance of corn seeds by soaking seeds, and the effect in Example 2 is the best.

[0159] In addition, root length means stronger water absorption capacity, and appropriate water stress can promote the growth of corn seed radicle and hypocotyl, while high-intensity water stress inhibits growth, and the hypocotyl inhibition effect is more obvious. In Examples 1 to 5, five different drought-resistant treatment agents are used, and the seed soaking effect in Example 2 is the best.

[0160] Water stress reduces the germination rate of corn seeds, hinders the growth of radicle and plumule and the accumulation of dry matter, but the corn seeds treated with the drought-resistant agent in Examples 1 to 5 have obvious differences in drought resistance compared with the control example;

[0161] Under water stress, the seeds soaked with the drought-resistant agent in Examples 1 to 5 still maintain a high germination rate, thereby reducing the damage of drought stress to the radicle and plumule of the seeds;

[0162] It can be seen from this that 1 weight part of drought-resistant rooting powder, CaCl 2 The best effect is achieved by a composition of 1 part by weight, 5 parts by weight of polyethylene glycol, 3 parts by weight of gibberellins, 2 parts by weight of salicylic acid, 1 part by weight of ethephon and 2 parts by weight of methylcyclopropene; the soaked seeds can enhance the drought resistance in the seedling stage, which is beneficial to drought resistance and seedling protection.

[0163] The application of drought-resistant agents can reduce the opening of crops' stomata, inhibit transpiration, increase chlorophyll content, improve root activity, slow down soil water consumption, and enhance crops' drought resistance. Soaking corn seeds with calcium chloride can promote the germination rate of corn seeds and increase corn yields. Soaking seeds with appropriate concentrations of rooting powder, CaCl2, polyethylene glycol, gibberellin, salicylic acid, ethephon, and methylcyclopropene can all improve corn seeds' drought resistance.

[0164] In addition, according to the subsequent experimental data, it was concluded that under certain stress conditions, the physiological and biochemical characteristics of corn seeds will change. For example, the content of malondialdehyde, free proline, and soluble protein will increase. This is a manifestation of plant adaptability to adverse stress and can be used as an indicator of corn seed resistance. Under the simulated drought stress environment, it was found that compared with corn seeds that were not treated with drought stress, the content of malondialdehyde, proline, and soluble protein in corn seeds had changed to varying degrees. The malondialdehyde content showed a downward trend, while the content of soluble protein and free proline showed an upward trend. The chlorophyll a content increased while the chlorophyll b content decreased, and the chlorophyll a / b value increased, indicating that seed soaking can improve the drought resistance of corn seeds.

[0165] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for drought resistance treatment of corn seeds, characterized in that: The specific steps include: Corn seeds were selected for germination test. After surface disinfection with 0.1% sodium hypochlorite for 10 minutes, they were rinsed several times with small amounts of distilled water until the smell of sodium hypochlorite disappeared. The seeds were randomly divided into several portions and soaked in different concentrations of drought-resistant treatment agents; Determination of germination rate, germination potential and germination index.

2. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: 1-5 parts by weight of rooting powder, 1-10 parts by weight of CaCl2, 5-15 parts by weight of polyethylene glycol, 3-8 parts by weight of gibberellin, 2-10 parts by weight of salicylic acid, 1-3 parts by weight of ethephon and 2-5 parts by weight of methylcyclopropene.

3. The drought resistance treatment method for corn seeds according to claim 2, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: The invention comprises 1 part by weight of rooting powder, 1 part by weight of CaCl2, 5 parts by weight of polyethylene glycol, 3 parts by weight of gibberellin, 2 parts by weight of salicylic acid, 1 part by weight of ethephon and 2 parts by weight of methylcyclopropene.

4. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: The composition includes 2 parts by weight of rooting powder, 3 parts by weight of CaCl2, 6 parts by weight of polyethylene glycol, 4 parts by weight of gibberellin, 4 parts by weight of salicylic acid, 1.5 parts by weight of ethephon and 2.5 parts by weight of methylcyclopropene.

5. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: The composition includes 3 parts by weight of rooting powder, 5 parts by weight of CaCl2, 7 parts by weight of polyethylene glycol, 5 parts by weight of gibberellin, 5 parts by weight of salicylic acid, 2 parts by weight of ethephon and 3 parts by weight of methylcyclopropene.

6. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: The invention comprises 45 parts by weight of rooting powder, 8 parts by weight of CaCl2, 10 parts by weight of polyethylene glycol, 6 parts by weight of gibberellin, 7 parts by weight of salicylic acid, 2.5 parts by weight of ethephon and 4 parts by weight of methylcyclopropene.

7. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The drought-resistant treatment agent is composed of the following raw materials in parts by weight: The invention comprises 5 parts by weight of rooting powder, 10 parts by weight of CaCl2, 15 parts by weight of polyethylene glycol, 8 parts by weight of gibberellin, 10 parts by weight of salicylic acid, 3 parts by weight of ethephon and 5 parts by weight of methylcyclopropene.

8. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: The calculation formulas for germination rate, germination potential and germination index are as follows: Germination rate: G = (n / N) * 100% where n is the number of germinated seeds and N is the number of seeds tested; Germination potential: GP = (a / N) * 100% where a is the number of germinated seeds within a specified time; Germination index: Where: GT is the number of seeds germinated within T days, and DT is the corresponding germination days.

9. The method for drought resistance treatment of corn seeds according to claim 1, characterized in that: It also includes a measure of vitality, where: GI=GP×S Where: S is the average fresh weight of each plant within the specified time.

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