A composite seedling cultivation method of Pterocarpus shashii-host

Abstract

The present invention discloses a composite seedling raising method for a host plant of the saburomys saburomys, comprising the following steps: selecting a non-woven bag or a plug tray as a culture container; taking a mixture of garden soil, nutrient soil, fine sand, and vermiculite as a matrix; selecting saburomys saburomys seeds with full particles, soaking them with gibberellins, washing them with distilled water, and sowing them after air drying; watering the matrix thoroughly before sowing; mixing the saburomys saburomys seeds with fine sand, and then sowing them on the soil surface of the matrix to moisten the soil surface; sowing wheat seeds or crabgrass seeds in the matrix sown with the saburomys saburomys; and managing light and nutrients: when the saburomys saburomys grows to the cotyledon stage and the true leaf stage, it needs to be cultured under high light intensity conditions and irrigated with seaweed nutrient solution and rooting powder. The composite seedling raising method for the saburomys ...

Description

Technical Field

[0001] The invention belongs to the technical field of medicinal plant cultivation, and particularly relates to a composite seedling cultivation method of Pterocarpus sabdariffa and a host plant. Background Art

[0002] Monochasma savatieri (French.ex Maxim) is the dried whole herb of Monochasma savatieri, a plant of the Scrophulariaceae family. Its dense, cottony, grayish-white hairs give it a grayish-white appearance, earning it the nickname "white-headed gentian." Monochasma savatieri plants can reach heights of 20-30 cm, with stems dying every other year. Its underground rhizomes are thick, short, and lignified, with densely developed fibrous roots. Its stem leaves are densely packed, alternately arranged and oblong-lanceolate to linear-lanceolate in shape.

[0003] Deer antler grass (Shapirossa shapirossa) is found in Zhejiang, Fujian, Jiangxi, Hubei, and Hunan provinces of my country. It grows among weeds in sunny areas of hillside woodlands and is also found under Masson pine forests. It is a perennial, semi-parasitic herb, and its entire plant is used as medicine. According to the Jiangxi Herbal Medicine and the Hangzhou Medicinal Plants, the entire plant has the effects of clearing heat and detoxifying, cooling blood, and stopping bleeding. It has a wide range of clinical applications, such as treating colds, wind-heat coughs and asthma, toothaches, rheumatic bone pain, thrush in children, and bleeding from trauma.

[0004] In recent years, the pharmaceutical market has seen increasing demand for Deer Antler Grass (Shapiro's antler grass). Due to environmental damage and overharvesting, the amount of Deer Antler Grass growing in the wild is decreasing. However, artificial cultivation of Deer Antler Grass suffers from low seedling survival rates and a long growth cycle.

[0005] Prior art discloses a potted method for growing P. saburoides, which parasitizes the plant on crabgrass to increase its seed germination rate. However, this method requires the host plant to be growing stably and well before sowing the seeds. This means the plant must first be cultivated before sowing. If the host plant withers, the plant will not be able to successfully reproduce. Summary of the Invention

[0006] In order to address the deficiencies of the prior art, the present invention provides a composite seedling cultivation method of Pterocarpus serratus-host, by establishing a composite seedling cultivation system of Pterocarpus serratus and gramineous plants such as wheat or crabgrass, thereby improving the seedling growth speed of Pterocarpus serratus and promoting the artificial large-scale cultivation of Pterocarpus serratus.

[0007] The technical effects to be achieved by the present invention are achieved through the following technical aspects:

[0008] A composite seedling cultivation method of Pterocarpus sabdariffa-host comprises the following steps:

[0009] S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container;

[0010] S2. Substrate preparation: Mix garden soil, nutrient soil, fine sand, and vermiculite in a volume ratio of 1:1.5 to 2:1:1 as the substrate and place it in a culture container;

[0011] S3. Treatment of Pelvis saburoides seeds: Select plump Pelvis saburoides seeds, soak them in gibberellin for 24–30 hours, rinse them 3–4 times with distilled water, and air-dry them before sowing.

[0012] S4 sowing: Before sowing, first water the substrate wet thoroughly; the S3 treated antler grass seeds and sand by volume ratio of 1: 8 to 20 ratio after mixing, and then evenly sowed on the soil surface of the substrate, with a micro-sprinkler to moisten the soil surface;

[0013] S5. Planting host: Sow wheat seeds or crabgrass seeds, or both wheat seeds and crabgrass seeds, in the substrate sown with S. serrata.

[0014] S6. Light and nutrient management: When the safflower antler grass reaches the cotyledon and true leaf stages, it needs to be cultivated under high light intensity conditions;

[0015] For the Sha's antler grass in the cotyledon stage and true leaf stage, seaweed nutrient solution and rooting powder need to be applied.

[0016] In some embodiments, the sowing step in S4 further includes the following steps:

[0017] S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water it 1 to 2 times every 1 to 2 days using a micro sprinkler.

[0018] In some embodiments, in each non-woven bag or each cell, the sowing ratio of the Herba Salviae Miltiorrhizae seeds to the wheat seeds or the crabgrass seeds is 8-10:1.

[0019] In some embodiments, in S5, the time of planting host wheat or crabgrass should be at least one month after sowing P. saburoi.

[0020] In some embodiments, in S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

[0021] In some embodiments, in S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is thoroughly moistened with the diluted carbendazim aqueous solution.

[0022] In some embodiments, in S3, the seeds of P. saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

[0023] In some embodiments, in the light management of S6, the saburo velvet in the cotyledon stage and the true leaf stage needs to be cultured under high light intensity conditions, and the high light intensity conditions are 5000-10000 Lux; if the natural light is insufficient, supplementary light treatment is required.

[0024] In some embodiments, in the nutrient management of S6, the seaweed nutrient solution is a 200-fold dilution of concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

[0025] In some embodiments, as the host of P. saburoi, the wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; the crabgrass is an annual herbaceous plant of the genus Digitaria in the Poaceae family;

[0026] Planting the host plant one month after sowing the safflower can:

[0027] Sow wheat seeds and crabgrass seeds simultaneously;

[0028] Or you can sow wheat seeds first, and then sow crabgrass seeds after 10-20 days;

[0029] Or you can sow crabgrass seeds first, and then sow wheat seeds after 10-20 days;

[0030] Or just broadcast wheat seeds or crabgrass seeds.

[0031] In summary, the present invention has at least the following benefits:

[0032] 1. The composite seedling cultivation method of the host plant of the saburo velvet provided by the present invention establishes a composite seedling cultivation system of the saburo velvet and the gramineous plants wheat or crabgrass. The time for planting the host wheat or crabgrass needs to be at least one month after the sowing of the saburo velvet, ensuring that the composite seedling cultivation is carried out after the saburo velvet and the wheat or crabgrass establish a parasitic relationship. On the one hand, this composite seedling cultivation method improves the seed germination rate of the saburo velvet and on the other hand, it improves the artificial cultivation success rate and seedling breeding speed of the saburo velvet, so as to ensure an adequate supply of the saburo velvet materials.

[0033] 2. The composite seedling cultivation method of the host plant provided by the present invention successfully realizes the artificial cultivation of the saburo velvet grass by using a non-woven bag or a plug tray planting method. The cultivation method is simple to operate, the planted saburo velvet grass grows well, and can promote the biomass accumulation of the saburo velvet grass, which is conducive to large-scale artificial cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 The composite seedling is formed by the saburo velvet grass of the present invention and a herbaceous host.

[0035] Figure 2 The composite seedlings are the saburo's velvet grass and a herbaceous host plant transplanted into a greenhouse.

[0036] Figure 3 The composite seedlings are obtained by transplanting the saburo velvet grass and a herbaceous host to a wild nurturing mountain. DETAILED DESCRIPTION

[0037] To facilitate understanding of the present invention, the present invention will be described more fully below in conjunction with the accompanying drawings and specific examples. Preferred embodiments of the present invention are provided in the examples. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of the present invention.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0039] References in this specification to "one embodiment," "an embodiment," "an exemplary embodiment," etc., mean that the embodiment being described is not necessarily one that includes a particular feature, structure, or characteristic. References to specific features, structures, or characteristics are included. Furthermore, such phrases do not necessarily refer to the same embodiment. Furthermore, when specific features, structures, or characteristics are described in connection with certain embodiments, it is within the knowledge of those skilled in the art to consider the effects of applying the features, structures, or characteristics to other embodiments, whether or not explicitly described.

[0040] Values ​​expressed as ranges should be interpreted in a flexible manner to include not only the values ​​explicitly listed as limits of the range, but also all individual values ​​or subranges included within the range, as if each value and subrange were clearly recited. For example, a (mass) concentration range of "about 0.1% to about 5%" should be interpreted as including not only the explicitly listed values ​​of about 0.1% to about 5% by weight, but also the individual (mass) concentrations (e.g., 1%, 2%, 3%, and 4%) and subranges (e.g., 0.1% to 0.5%, 1.1% to 2.2%, and 3.3% to 4.4%) within the specified range.

[0041] As described herein, unless stated otherwise, the terms "a" and "an" are used to include one or more than one, and the term "or" is used to refer to a non-exclusive "or". In addition, when a term or terms are used herein without being otherwise qualified, they should be understood to be used for descriptive purposes only and not for limiting purposes. In addition, all publications, patents, and patent documents mentioned in the specification are incorporated herein by reference in their entirety, as if individually incorporated by reference. If there is a discrepancy between the usage of this document and those documents incorporated by reference, the usage in the cited references should be considered to supplement that of this document. In the event of irreconcilable inconsistencies, the usage in this document shall prevail.

[0042] A composite seedling cultivation method of Pterocarpus sabdariffa-host comprises the following steps:

[0043] S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container;

[0044] S2. Substrate preparation: Mix garden soil, nutrient soil, fine sand, and vermiculite in a volume ratio of 1:1.5 to 2:1:1 as the substrate and place it in a culture container;

[0045] S3. Treatment of Pelvis saburoides seeds: Select plump Pelvis saburoides seeds, soak them in gibberellin for 24–30 hours, rinse them 3–4 times with distilled water, and air-dry them before sowing.

[0046] S4. Sowing: Before sowing, thoroughly moisten the substrate with water. Mix the treated velvet antler grass seeds from S3 with fine sand at a volume ratio of 1:8-20, then evenly spread over the soil surface of the substrate. Moisten the soil surface with a micro-sprinkler.

[0047] S5. Planting host: Sow wheat seeds or crabgrass seeds, or both wheat seeds and crabgrass seeds, in the substrate sown with S. serrata.

[0048] S6. Light and nutrient management: When the safflower antler grass reaches the cotyledon and true leaf stages, it needs to be cultivated under high light intensity conditions;

[0049] For the Sha's antler grass in the cotyledon stage and true leaf stage, seaweed nutrient solution and rooting powder need to be applied.

[0050] In this combined seedling cultivation method for P. sabendula and its host, P. sabendula is artificially planted in planting bags. Specifically, the plants are concentrated in non-woven bags or plug trays, which are filled with a substrate composed of garden soil, nutrient soil, fine sand, and vermiculite, and are thoroughly watered with a carbendazim solution.

[0051] The inventors discovered that P. saburoides has a companion relationship with wheat or crabgrass, meaning that it thrives in these environments. The inventors also discovered that if the host wheat or crabgrass grows to a certain height, it can hinder the germination of P. saburoides seeds and the growth of seedlings.

[0052] Therefore, during the planting process, before sowing host wheat or crabgrass, it is necessary to plant Deer Antler Grass first. Only after Deer Antler Grass has grown stably and well, can host wheat or crabgrass be sown, thereby establishing a stable parasitic relationship between Deer Antler Grass and host wheat or crabgrass, so that the seedlings of Deer Antler Grass can grow quickly and stably and the plants can be strong.

[0053] The seeding step in S4 further includes the following steps:

[0054] S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water it 1 to 2 times every 1 to 2 days using a micro sprinkler.

[0055] In each non-woven bag or cell, the ratio of sabdariffa seeds to wheat or crabgrass seeds is 8 to 10:1. This sowing ratio balances the planting density of the parasite sabdariffa and its host, wheat or crabgrass, ensuring rapid growth of the sabdariffa after germination.

[0056] In step S5, the time for planting host wheat or crabgrass should be at least one month after the sowing of the safflower herb.

[0057] After experiments and screening, the inventors found that planting wheat or crabgrass at least one month after sowing the seeds of the saburo velvet grass is conducive to the establishment of a parasitic relationship between the saburo velvet grass and wheat or crabgrass, as well as the subsequent biomass accumulation of the saburo velvet grass.

[0058] Wherein, in steps S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

[0059] Wherein, in step S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is thoroughly moistened with the diluted carbendazim aqueous solution.

[0060] Wherein, in step S3, the seeds of Pterocarpus saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

[0061] Among them, in the light management of step S6, the cotyledon stage and true leaf stage of the sabdariffa need to be cultivated under high light intensity conditions, which are 5000-10000 Lux; if the natural light is not enough, supplementary light treatment is required.

[0062] Under the cultivation conditions of higher light intensity (5000-10000 Lux), the growth of S. saburoides in the cotyledon and true leaf stages can be significantly promoted, and the biomass accumulation of wheat or crabgrass can be significantly increased, while also avoiding excessive moisture content in the substrate.

[0063] In the nutrient management of step S6, the seaweed nutrient solution is a 200-fold dilution of the concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

[0064] As the host of P. shassoni, wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; crabgrass is an annual herbaceous plant of the genus Digitaria in the Poaceae family;

[0065] Plant the host plant one month after sowing the sedge antler grass. You can sow wheat seeds and crabgrass seeds at the same time; or sow wheat seeds first and then sow crabgrass seeds after a gap of 10-20 days; or sow crabgrass seeds first and then sow wheat seeds after a gap of 10-20 days; or only sow wheat seeds or crabgrass seeds.

[0066] Example 1

[0067] Experimental study on the effects of different substrate ratios and seedling containers on the germination of Pterocarpus sabdariffa

[0068] A method for growing saburo velvet by composite seedling cultivation is carried out according to the following steps:

[0069] (1) Substrate ratio and seedling container selection

[0070] Two treatment groups were set up with substrate ratios: G1 [garden soil: nutrient soil: fine sand = 1:1:1 (v / v)] and G2 [mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1 (v / v)].

[0071] Three treatment groups were set up in the seedling raising containers: D1 [black seedling raising pot 3 cm × 6 cm (diameter × height)], D2 [small non-woven bag 4 cm × 12 cm (diameter × height)], and D3 [large non-woven bag 6 cm × 12 cm (diameter × height)].

[0072] (2) Sowing time of Pterocarpus serrata and its host plant

[0073] Twenty seeds of Psoralea corylifolia were sown in each treatment group on October 29, 2023, and three seeds of Crabgrass were sown on November 29.

[0074] (3) Cultivation and management

[0075] During the germination process of the seeds of Pterocarpus serrata, water was sprayed to keep them moist. The non-woven bag was placed in a plastic seedling tray (46 cm × 46 cm × 10 cm, length × width × height) and covered with a film. The film was removed after the seedlings were all grown.

[0076] Seaweed nutrient solution (1:200 dilution): Spray an equal amount evenly into each non-woven bag and follow standard management. Observe the germination of P. serrata seeds every 24 hours and count the number of germinations. The germination test is concluded when no new seedlings emerge from the soil for three consecutive days. The results are shown in Table 1.

[0077] Table 1 Germination indexes of Pterocarpus serrata treated with different substrates and seedling containers

[0078]

[0079]

[0080] As shown in Table 1, the germination rate, germination potential, germination index, and vitality index of the safflower herb in the G2D3 treatment were significantly higher than those in the other treatments, followed by the G2D2 treatment. The germination rate in the G2D3 treatment was 14.5 percentage points higher than that in the G1D2 treatment. The lowest germination rate, germination potential, germination index, and vitality index were observed in the G1D2, G1D1, G1D1, and G1D1 treatments, respectively.

[0081] Example 2

[0082] Experimental study on the effects of supplementary light and rooting agent on the growth indexes of Pterocarpus sabdariffa at the cotyledon stage

[0083] A method for growing saburo velvet by composite seedling cultivation is carried out according to the following steps:

[0084] (1) Selection of seedling container and substrate treatment

[0085] Small non-woven bags with a size of 4cm×12cm (diameter×height) were selected for composite seedling cultivation of Deer Antler Grass and host Digitaria, and garden soil, nutrient soil (Cuiyun Liangtu), fine sand and vermiculite were selected as the substrate, with the ratio of mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0086] (2) Seed treatment

[0087] Soak the seeds of Psoralea corylifolia in an 800 mg / L gibberellin solution for 24 hours, rinse with distilled water 3-4 times, and air-dry. Air-dry the seeds of Crabgrass and store them in a cool, shady place until ready for use.

[0088] (3) Seed planting

[0089] Plant the seeds of the sabdariffa first, sowing 20 seeds in each seedling container, and sow the crabgrass seeds one month later, sowing 3 seeds in each seedling container.

[0090] (4) Cultivation and management

[0091] LED lights (5000-8000 Lux) were placed approximately 40 cm above the seedling containers to provide supplemental lighting, with the lighting period ranging from 7:30 AM to 7:30 PM. Concentrated seaweed nutrient solution (diluted 200-fold) was also applied once daily, with 40 mL added to each bag of seedling container. The seedlings of Psoralea corylifolia were also treated with rooting powder at a concentration of 15 mg / L, once a week for five consecutive weeks.

[0092] This method of cultivating the seedlings of Sha's deer antler grass with its host, Digitaria sanguinea, through technical treatments such as substrate ratio, seedling container selection, LED light supplementation, and fertilization, has resulted in Sha's deer antler grass having extremely good seedling survival rate, accumulated biomass, and number of suckers. Promoting this technology can effectively protect Sha's deer antler grass, prevent the consumption of wild resources, and ensure the supply of Sha's deer antler grass in the market.

[0093] In the cultivation management, the conditions of supplementary light and rooting agents were investigated, and the results are shown in Table 2.

[0094] Table 2 Effects of supplementary light and rooting agents on the growth indexes of Pterocarpus serrata at the cotyledon stage ( n=10)

[0095]

[0096] Note: Different lowercase letters in the same column indicate significant differences, P < 0.05.

[0097] LL is the low-light control, which is the natural light intensity in the laboratory (500-1500 Lux); HL is the supplemental light treatment, which is supplemental light (5000-8000 Lux) provided by an LED light approximately 40 cm above the non-woven bag. W1 is watered once a day with a 200-fold diluted seaweed nutrient solution, 40 mL per bag each time; W2 is watered once every two days with a 200-fold diluted seaweed nutrient solution, 40 mL per bag each time. R0 is the distilled water control, treated once a week; R15 is treated once a week with 15 mg / L rooting powder.

[0098] The analysis of variance results in Table 2 show that, with the exception of nutrient solution, which had no significant effect on the number of lateral roots in the cotyledonary stage, the presence or absence of supplemental light, rooting powder, and nutrient solution all had significant effects on leaf and root growth. The effect of supplemental light, in particular, reached a P < 0.001 level in most cases. Interactions among the three treatments were also significant. Regarding root length, the influence of each factor ranked rooting powder > supplemental light > nutrient solution. For other parameters, the influence of each factor ranked light > rooting powder > nutrient solution.

[0099] Example 3

[0100] Experimental study on the effects of different fertilizer treatments on the growth of Pterocarpus sabdariffa

[0101] A method for growing saburo velvet by composite seedling cultivation is carried out according to the following steps:

[0102] (1) Selection of seedling container

[0103] Small non-woven bags with a size of 4cm×12cm (diameter×height) were selected for composite seedling cultivation of Pterocarya serrata and Digitaria sanguinalis.

[0104] (2) Matrix treatment

[0105] Choose garden soil, nutrient soil, fine sand and vermiculite, with the ratio of mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0106] (3) Treatment of Pterocarpus serrata seeds

[0107] The seeds of Pterocarpus sabdariffa were soaked in 800 mg / L gibberellin solution for 24 h, disinfected with mercuric chloride for 8 min, washed with distilled water and dried.

[0108] (4) Seeding of Deer Antler Grass and Crabgrass

[0109] Plant the seeds of the sabdariffa first, sowing 20 seeds in each seedling container, and sow the crabgrass seeds one month later, sowing 3 seeds in each seedling container.

[0110] (5) Water management

[0111] After sowing, water the plants with a micro-sprinkler every 1-2 days, observing them frequently. During germination, ensure adequate water supply, keeping the substrate moist but not waterlogged. After the seedlings have established growth, water them every 2-4 days, depending on weather conditions, thoroughly moistening the substrate with each watering. Observe soil moisture daily at 8:00 AM and 5:00 PM, maintaining a relative moisture content of 75%-80% for each treatment.

[0112] (3) Seedling management

[0113] Five treatments were set up: a blank control (CK, equivalent to the amount of water), a seaweed nutrient solution treatment (HZ), a MS culture solution treatment (MS), a low-concentration NPK treatment (LN) (a mixture of seaweed nutrient solution, 0.5 g / L urea, and 0.2 g / L potassium dihydrogen phosphate), and a high-concentration NPK treatment (HN) (a mixture of seaweed nutrient solution, 2 g / L urea, and 0.8 g / L potassium dihydrogen phosphate). Each treatment contained 15 non-woven bags. The bags were uniformly maintained in the greenhouse at Nanjing Agricultural University. The first treatment was conducted around 6:00 PM after seedling emergence, with approximately 40 mL per bag. Treatments were continued every 7 days for a total of 63 days. The results are shown in Table 3.

[0114] Table 3 Effects of different fertilizer concentrations on the growth indexes of Pterocarpus sabdariffa ( n=15)

[0115]

[0116]

[0117] Note: Different lowercase letters in the same column indicate significant differences. “-” indicates missing data due to the death of P. saburoides seedlings.

[0118] Table 3 shows the effects of different fertilization treatments on the growth parameters of P. serrata. Both HZ and LN treatments promoted the growth of P. serrata, but MS and HN treatments significantly inhibited its growth, even causing lethality after prolonged treatment. Root length and plant height in HZ treatments were significantly higher than those in the other treatments (p < 0.05), increasing by 24.87% and 95.74%, respectively, compared to CK. Significant differences in leaf length and width were also observed between CK, HZ, and LN treatments (p < 0.05). HZ treatments significantly increased leaf length and width by 20.78% and 28.61%, respectively, compared to CK. These data indicate that HZ significantly promoted the growth of P. serrata, while LN treatments attenuated the effects of HZ on the growth of P. serrata and even inhibited its growth.

[0119] Example 4

[0120] An experiment to investigate the effects of supplementary light and rooting agents on the growth indicators of Pterocarpus serratus at the true leaf stage (wheat as host) A method for growing Pterocarpus serratus in composite seedling cultivation was carried out in the following steps:

[0121] (1) Selection of seedling container and substrate treatment

[0122] Small non-woven bags with a size of 4cm×12cm (diameter×height) were selected for composite seedling cultivation of Pterocarya serrata and host wheat. Garden soil, nutrient soil (Cuiyunliang soil), fine sand and vermiculite were selected as the substrate, with the ratio of garden soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0123] (2) Seed treatment

[0124] Soak the seeds of Pterocarpus sabdariffa in an 800 mg / L gibberellin solution for 24 hours, rinse with distilled water 3-4 times, and air-dry. Air-dry the wheat seeds and store them in a cool, shady place until ready for use.

[0125] (3) Seed planting

[0126] Prioritize planting Sha's Deer Antler Grass seeds, sow 20 seeds in each seedling container, and sow wheat seeds one month later, sow 2 seeds in each seedling container.

[0127] (4) Cultivation and management

[0128] LED lights (5000-8000 Lux) were placed approximately 40 cm above the seedling containers to provide supplemental lighting, with the lighting period ranging from 7:30 AM to 7:30 PM. Concentrated seaweed nutrient solution (diluted 200-fold) was also applied once daily, with 40 mL added to each bag of seedling container. The seedlings of Psoralea corylifolia were also treated with rooting powder at a concentration of 15 mg / L, once a week for five consecutive weeks.

[0129] This method of cultivating co-seedlings of Pyrola serrata and host wheat, through technical treatments such as substrate ratio, seedling container selection, LED light supplementation, and fertilization, has resulted in extremely excellent seedling survival rate, biomass accumulation, and number of haustoria of Pyrola serrata. Promoting this technology can effectively protect Pyrola serrata, prevent the consumption of wild resources, and ensure the supply of Pyrola serrata in the market.

[0130] In the cultivation management, the conditions of supplementary light and rooting agents were investigated, and the results are shown in Table 4.

[0131] Table 4 Effects of supplementary light and rooting agents on the dry and fresh weights of Pterocarpus serrata at the true leaf stage ( n=10)

[0132]

[0133] Note: Wheat is the host plant. Different lowercase letters in the same column indicate significant differences, p < 0.05.

[0134] LL is the low-light control, which is the natural light intensity in the laboratory (500-1500 Lux); HL is the supplemental light treatment, which is supplemental light (5000-8000 Lux) provided by an LED light approximately 40 cm above the non-woven bag. W1 is watered once a day with a 200-fold diluted seaweed nutrient solution, 40 mL per bag each time; W2 is watered once every two days with a 200-fold diluted seaweed nutrient solution, 40 mL per bag each time. R0 is the distilled water control, treated once a week; R15 is treated once a week with 15 mg / L rooting powder.

[0135] Table 4 shows the effects of different light intensities, nutrient solution frequencies, and rooting powder concentrations on the fresh and dry weights of P. saburoides at the true leaf stage under wheat. Both the biomass and dry weight ratio of P. saburoides in the supplementary light treatment were significantly higher than those in the unsupplemented light treatment. The highest fresh and dry weights occurred in the HL+W1+R15 treatment (i.e., high light intensity supplementation, 15 mg / L rooting powder once a week, and nutrient solution application once a day). This treatment achieved a 14.75-fold increase in dry weight compared to the LL+W1+R0 treatment, and an 8.14-fold increase in fresh weight compared to the LL+W1+R15 treatment. Among the supplementary light treatments, the dry weight of the W1+R15 treatment was significantly higher than that of the W2+R15 treatment, while the dry weight of P. saburoides in the W1+R0 treatment was 23.80% higher than that of the W2+R0 treatment, although the differences did not reach significance. This suggests that increasing the frequency of nutrient solution application can enhance biomass accumulation in P. saburoides at the true leaf stage. In the treatment without light supplementation, the maximum fresh and dry weight appeared in the W2+R15 treatment. The HL+W1+R15 treatment had the highest dry weight, which was significantly higher than that of the treatment without light supplementation.

[0136] These results indicate that supplemental light has a significant effect on the biomass accumulation of P. saburoides during the true leaf stage when wheat is used as the host. In addition to supplemental light, daily watering with concentrated seaweed nutrient solution and weekly treatment with a 15 mg / L rooting powder solution further promoted the growth of P. saburoides.

[0137] The above description is merely an example and illustration of the structure of the present invention. Although the description is relatively specific and detailed, it should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art may make various modifications and improvements without departing from the scope of the present invention, and such obvious alternatives are all within the scope of protection of the present invention.

Claims

1. A composite seedling cultivation method of Pterocarpus sabdariffa-host, characterized in that: The following steps are involved: S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container; S2. Substrate Preparation: Mix garden soil, nutrient soil, fine sand, and vermiculite in a volume ratio of 1:1.5 to 2:1:1 as the substrate and place in a culture container. S3. Seed treatment of P. saburoides: Select plump seeds of P. saburoides, soak them in gibberellin for 24–30 hours, rinse them three to four times with distilled water, air-dry them, and sow them immediately. S4 sowing: Before sowing, first water the substrate wet thoroughly; the S3 treated antler grass seeds and sand by volume ratio of 1: 8 to 20 ratio after mixing, and then evenly sowed on the soil surface of the substrate, with a micro-sprinkler to moisten the soil surface; S5. Planting host: Sow wheat seeds or crabgrass seeds, or both wheat seeds and crabgrass seeds, in the substrate sown with sabdariffa. S6. Light and nutrient management: When the safflower herb reaches the cotyledon and true leaf stages, cultivate it under high light intensity conditions. For the safflower antler grass in the cotyledon stage and true leaf stage, apply seaweed nutrient solution and rooting powder; In said S5, the time of planting host wheat or crabgrass should be at least one month after sowing the sabdariffa; In the light management of S6, the high light intensity condition is 5000-10000 Lux; if the natural light is insufficient, supplementary light processing is required; In the nutrient management of S6, the seaweed nutrient solution is a 200-fold dilution of concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

2. The composite seedling cultivation method of Pterocarpus sabdariffa-host according to claim 1, characterized in that: In the sowing step of S4, the following steps are also included: S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water it 1 to 2 times every 1 to 2 days using a micro sprinkler.

3. The composite seedling cultivation method of Pterocarpus sabdariffa-host according to claim 1, characterized in that: In each non-woven bag or each cell, the sowing ratio of the sabdariffa seeds to the wheat seeds or crabgrass seeds is 8-10:

1.

4. The composite seedling cultivation method of Pterocarpus sabdariffa-host according to claim 1, characterized in that: In S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

5. The composite seedling cultivation method of Pterocarpus sabdariffa-host according to claim 1, characterized in that: In S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is thoroughly moistened with the diluted carbendazim aqueous solution.

6. The composite seedling cultivation method of Pterocarpus saburoi-host according to claim 1, characterized in that: In S3, the seeds of Pterocarpus saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

7. The composite seedling cultivation method of Pterocarpus saburoi-host according to claim 1, characterized in that: As the host of the shamrock grass, the wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; the crabgrass is an annual herbaceous plant of the genus Digitaria in the Poaceae family; In said S5, the host plant is planted one month after sowing the saburo velvet, and further: Sow wheat seeds first, and then sow crabgrass seeds 10-20 days later; Alternatively, sow crabgrass seeds first and then sow wheat seeds 10-20 days later.

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