Cultivation method of Guangxi curcuma zedoary

Abstract

The invention belongs to the field of traditional Chinese medicine cultivation, and particularly relates to a cultivation method of Guangxi curcuma zedoary. According to experiments on influences of phosphatic fertilizers with different concentrations on growth and development of the Guangxi curcuma zedoary, accumulation of dry matters and stress resistance, it is determined that when the application amount of exogenous phosphorus is 600mg / kg, the promotion effect on stem diameter, leaf width, leaf length, leaf number and plant height of the Guangxi curcuma zedoary is most remarkable.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine cultivation, and particularly relates to a cultivation method of Guangxi Curcuma zedoaria. Background Art

[0002] Guangxi Curcuma is a major herbal Chinese medicine and one of the "Ten Flavors of Guangxi" authentic medicinal materials. It mainly contains two major chemical components: volatile oils and curcuminoids, among which volatile oil sesquiterpenes are the main ones. Modern pharmacological studies have shown that it has anti-inflammatory, anti-tumor and liver-protecting effects. It is widely used in clinical research and treatment. With the further discovery of its medicinal potential, the social demand for it has further expanded. With the improvement of people's health awareness and the deepening of their understanding of the efficacy and value of Curcuma, the market demand continues to increase.

[0003] Phosphorus is an essential nutrient for plants. Reasonable application of phosphorus fertilizer can increase the ratio of underground to aboveground dry weight or fresh weight of plants, increase crop yields, and promote the development and growth of plant roots. It can significantly improve the growth and development of the roots of plants whose roots are medicinal parts, and has a significant effect on improving the quality and yield of medicinal materials.

[0004] A large amount of phosphorus is needed in the cultivation process of Curcuma zedoaria in Guangxi. Adequate phosphorus can promote its growth, maturity and increase yield. However, when Curcuma zedoaria is in different growth stages, different amounts of fertilizers have a crucial impact on the yield of Curcuma zedoaria. Fertilization is needed to promote the growth of tubers and rhizomes when the seedlings are tuber-forming. Seedlings that emerge late will be tender, and fertilization will cause the above-ground part to grow wildly, consuming most of the nutrients, and the underground tubers and rhizomes will be reduced. At present, there is a relative lack of research on the effects of the rational application of phosphorus fertilizers on Chinese medicinal materials, especially on ginger-related Chinese medicinal materials, which greatly restricts the development of precision and standardization of Curcuma zedoaria cultivation in Guangxi. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a cultivation method of Guangxi Curcuma zedoaria, wherein exogenous phosphorus is applied after the Curcuma zedoaria seedlings grow two leaves and one heart.

[0006] Furthermore, the exogenous phosphorus is an aqueous solution of potassium dihydrogen phosphate.

[0007] Furthermore, the concentration of the exogenous phosphorus is 100-1200 mg / kg.

[0008] Furthermore, the concentration of the exogenous phosphorus is 600-1200 mg / kg.

[0009] Furthermore, the concentration of the exogenous phosphorus is 600 mg / kg.

[0010] Furthermore, the exogenous phosphorus is applied by root irrigation.

[0011] The present invention has the following beneficial effects:

[0012] The invention provides the phosphorus concentration that is most conducive to promoting the growth of Guangxi Curcuma zedoaria seedlings, can better guide the scientific, reasonable and efficient application of phosphorus fertilizers in the cultivation and production of Guangxi Curcuma zedoaria, and is of great significance to the development of the yield and quality of ginger family Chinese medicinal materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 CAT activity results;

[0015] Figure 2 POD activity results;

[0016] Figure 3 SOD activity results;

[0017] Figure 4 Malondialdehyde content results;

[0018] Figure 5 Results of soluble sugar and soluble protein content;

[0019] Figure 6 Chlorophyll content results;

[0020] Figure 7 Growth indicator results;

[0021] Figure 8 Bioassignment results. DETAILED DESCRIPTION

[0022] Now, various exemplary embodiments of the present invention are described in detail. The methods in the examples are conventional methods unless otherwise specified, and the reagents used are conventional commercially available reagents or reagents prepared by conventional methods unless otherwise specified. This detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, features and embodiments of the present invention.

[0023] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0024] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0025] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0026] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0027] Example

[0028] The experiment was set up in the experimental greenhouse of the Guangxi Medicinal Botanical Garden Research Base, and the annual Guangxi Curcuma tubers were used as test materials for seedling cultivation. First, prepare the seedbed, select fine yellow heart soil and organic fertilizer 1:1 mix, put into 7*9cm seedling bags, water the seedbed thoroughly, and keep the soil in the seedbed loose, fertile and moist. Then select full and uniform Curcuma tubers, cut them into two pieces, and finally lay the cut Curcuma tubers flat in the seedling bag, cover them with a thin layer of soil, and water them again to facilitate tuber germination. Thereafter, water them every three days during the seedling stage, water the seedling bag thoroughly each time, and weed and loosen the soil in time to promote seedling growth. When the seedlings grow one leaf and one heart, they are transplanted. Select the Guangxi Curcuma zedoaria seedlings with uniform height and growth, carefully dig them out and plant them in pots (15×30cm). The matrix is ​​yellow heart soil: organic fertilizer (9:1). The weight of each pot soil is 6.5kg. Water it in time after transplanting to keep the soil moist. Then, after the Curcuma zedoaria seedlings grow two leaves and one heart, a potted test is carried out. 80 Guangxi Curcuma zedoaria seedlings with uniform growth are selected, 15 plants per group, divided into the control group P0 and the treatment group (P1, P3, P6, P12), and marked. The treatment group uses potassium dihydrogen phosphate solution as exogenous phosphorus, and the control group uses an equal amount of pure water instead. Fertilization is carried out after the transplant survives, and each index is measured one month after fertilization. The concentration of phosphorus fertilizer is set to: 0, 100, 300, 600mg / kg, 1200mg / kg. During the experiment, the rest of the management measures remain consistent between the treatments. Sampling was carried out for each treatment at different growth stages, and three replicates were set.

[0029] Collecting plant samples:

[0030] The plant measurement indicators include: growth index, dry matter weight, chlorophyll content, soluble sugar, soluble protein content, peroxidase content, superoxide dismutase content, catalase content, and malondialdehyde content.

[0031] Results and Analysis

[0032] ① When the exogenous phosphorus concentration is 300mg / kg~1200mg / kg, it has a significant promoting effect on the growth of Guangxi Curcuma, and when the exogenous phosphorus application amount is 600mg / kg, the promoting effect on the stem diameter, leaf width, leaf length, leaf number and plant height of Guangxi Curcuma is the most significant.

[0033] ② Applying high concentration of exogenous phosphorus can greatly increase the fresh weight and dry weight of Curcuma zedoaria, especially when the exogenous phosphorus application amount is 600mg / kg and 1200mg / kg.

[0034] ③With the increase of phosphorus concentration, the activities of antioxidant enzymes CAT, POD, and SOD gradually increased, reaching a small peak at 600 mg / kg. There were significant differences between 600 mg / kg and 1200 mg / kg and the control group, and the activity was maximum at 1200 mg / kg, indicating that the application of high concentration of exogenous phosphorus can maximize the resistance of Guangxi Curcuma to adverse environment.

[0035] ④ As the concentration of exogenous phosphorus gradually increased, the content of malondialdehyde gradually increased. When the concentration of exogenous phosphorus reached P6, the content of malondialdehyde in Curcuma zedoaria increased significantly, showing a stress effect, and its stress resistance decreased. When the concentration of exogenous phosphorus was between 300 mg / kg and 600 mg / kg, Curcuma zedoaria had the strongest stress resistance and suffered the least stress damage.

[0036] ⑤With the increase of exogenous phosphorus concentration, the content of soluble sugar and soluble protein in Curcuma zedoaria gradually increased, reaching the maximum when the exogenous phosphorus concentration was 600 mg / kg. At this time, the synthesis and metabolic activities of nutrients in Curcuma zedoaria were most active.

[0037] summary:

[0038] Different amounts of exogenous phosphorus fertilizer were applied to Curcuma zedoaria. When the exogenous phosphorus application amount was 300mg / kg~1200mg / kg, the morphological indicators of Curcuma zedoaria, such as plant height, stem thickness, and leaf number, were significantly promoted. When the exogenous phosphorus application amount was 600mg / kg, it promoted the dry matter accumulation of Curcuma zedoaria, and then showed a slight downward trend with the increase of exogenous phosphorus application. The chlorophyll content, soluble sugar and soluble protein content of Curcuma zedoaria leaves reached the maximum value when the exogenous phosphorus application amount was 600mg / kg, and the concentration continued to increase and showed a downward trend. When the exogenous phosphorus application amount was 1200mg / kg, the three antioxidant enzymes POD, SOD, and CAT reached the maximum value. Taking into account the comprehensive factors such as the growth status, dry matter accumulation and stress resistance of Curcuma zedoaria seedlings, it is recommended that the exogenous phosphorus application amount of Curcuma zedoaria in Guangxi be 600mg / kg.

[0039] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A cultivation method of Guangxi Curcuma zedoaria, characterized in that, Apply exogenous phosphorus after the Curcuma zedoaria seedlings have two leaves and one heart.

2. The cultivation method according to claim 1, characterized in that The exogenous phosphorus is an aqueous solution of potassium dihydrogen phosphate.

3. The cultivation method according to claim 1, characterized in that The concentration of the exogenous phosphorus is 100-1200 mg / kg.

4. The cultivation method according to claim 3, characterized in that: The concentration of the exogenous phosphorus is 600-1200 mg / kg.

5. The cultivation method according to claim 3, characterized in that: The concentration of the exogenous phosphorus is 600 mg / kg.

6. The cultivation method according to claim 1, characterized in that: The exogenous phosphorus is applied by root irrigation.

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