Breeding method for increasing milk yield of dairy cows

Abstract

The invention provides a breeding method for increasing the milk yield of dairy cows, and belongs to the technical field of dairy cow breeding. The method comprises the steps of refined feed preparation, young period management, perinatal period management, lactation period management and dry period management. The perinatal period management comprises the step of feeding perinatal period feed every day; the perinatal period feed comprises silage corn stalks, sweet potato leaves, refined feed, corn flour, soybean meal, molasses, nutrient elements and table salt; the nutrient elements comprise soybean oligopeptide, vitamin A, vitamin E, manganese sulfate, zinc sulfate, rumen protected methionine and rumen protected lysine; the lactation period management comprises the step of feeding lactation period feed every day, and the lactation period feed comprises silage corn stalks, sweet potato leaves, refined feed, corn flour, soybean meal, rumen bypass fat, table salt and nutritional auxiliaries; the nutritional additive comprises nicotinic acid, calcium carbonate, magnesium oxide, vitamin D and sodium bicarbonate; by means of the breeding method, the milk yield is high, and the milk quality is good.

Description

Technical Field

[0001] The invention belongs to the technical field of dairy cow breeding, and in particular relates to a breeding method for improving the milk production of dairy cows. Background Art

[0002] Milk, known as "white blood", is rich in calcium, which helps prevent osteoporosis, weak waist and knees caused by calcium deficiency. It is especially important for the bone development and height growth of adolescents. Milk can also provide high-quality protein, improve the body's immunity, and enhance muscle strength. Milk also has the effects of beautifying the skin, calming the nerves, and relieving physical fatigue. It is of great value in maintaining human health and balance.

[0003] With the development of science and technology and the improvement of people's living standards, people's demand for high-quality milk is increasing. As a decisive factor affecting milk production and quality, the breeding method of dairy cows has received more and more attention. In dairy cow farming, the milk production process can be roughly divided into the early lactation, peak lactation, mid-lactation, and late lactation stages; usually, the milk quality is better in the early lactation stage, the quality is relatively stable in the peak lactation stage, the output and quality will decline to a certain extent in the mid-lactation stage, and both the output and quality will decline seriously in the late lactation stage, and there will also be a problem of declining milk production and quality year by year; in order to increase milk production and milk quality, some farms use a large amount of antibiotics and drugs, but their long-term use will irritate the gastrointestinal tract, aggravate the decline in milk quality, and affect the health of dairy cows, ultimately resulting in a decline in milk production and quality.

[0004] CN109042491A provides a breeding method for increasing the milk production of dairy cows, specifically, playing music one hour before and after each feeding; using lights to supplement light in spring, autumn and winter, and feeding dairy cows with water at 38-40°C in winter; regularly dipping the teats of dairy cows, and feeding different feeds during the pregnancy preparation period, peripartum period, early lactation period, peak lactation period and late lactation period of dairy cows to ensure the nutritional needs of dairy cows; With this patented breeding method, each dairy cow can produce 55.6kg of milk per day, but the quality of the milk is poor, with low levels of milk protein and milk fat.

[0005] CN102919196A discloses a feeding method for improving the milk production and quality of dairy cows. For lactating dairy cows, before giving conventional feed, each dairy cow is given 5-100g of acidogenic Propionibacterium bacterial protein per day; when the acidogenic Propionibacterium bacterial protein is 5g / head / day, the lactose content is 5.1%, the milk fat content is 4.18%, and the average daily milk production is 41.2kg; when the acidogenic Propionibacterium bacterial protein is 100g / head / day, the lactose content is 8.88%, the milk fat content is 7.07%, and the average daily milk production is 53.4kg; It can be seen that in this feeding method, milk production and milk fat content are greatly affected by the dosage of acidogenic Propionibacterium somatic protein; when the production and milk fat content in milk are high, the dosage of acidogenic Propionibacterium somatic protein is as high as 100g / head / day, and the milk production in the middle and late lactation stages is still low. Summary of the invention

[0006] In order to solve the technical problems existing in the prior art, the present invention provides a breeding method for increasing the milk production of dairy cows, with high milk production, high milk protein and milk fat content in milk, uniform milk production of dairy cows, and still high milk production in the middle and late lactation stages.

[0007] In view of the above technical problems, the present invention adopts the following technical solutions: A breeding method for increasing milk production, which is as follows: 1. Preparation of refined feed (1) Extraction The dandelion, astragalus and tongcao are vacuum freeze-dried, the vacuum degree is controlled at 0.05-0.08MPa, the temperature is -42 to -38°C, the freeze-drying time is 46-50h, after the freeze-drying is completed, after returning to room temperature, crushing through a 110-130 mesh sieve to obtain a mixed powder; 6-10 times the weight of ethanol solution is added to the mixed powder for an ultrasonic extraction, the ultrasonic power is 492-510W, the ultrasonic time is 47-53min, the ultrasonic frequency is 37-43kHz, the ultrasonic temperature is 45-50°C, after the ultrasonic extraction is completed, separation , obtaining a primary ultrasonic extract and a solid extract; placing the solid extract in an ethanol solution of 8-12 times the mass for secondary ultrasonic extraction, the ultrasonic power is 510-530W, the ultrasonic time is 47-53min, the ultrasonic frequency is 37-43kHz, the ultrasonic temperature is 45-50°C, after the secondary ultrasonic extraction is completed, separation is performed to obtain a secondary ultrasonic extract; the primary ultrasonic extract and the secondary ultrasonic extract are mixed, stirred evenly, and then concentrated under reduced pressure at 48-52°C and 0.06-0.08MPa to 48-52% of the original volume to obtain a plant extract; The mass ratio of dandelion, astragalus and tongcao is 5.6-6.0:1.0-1.4:1.3-1.7; Therefore, the volume concentration of the ethanol solution is 68-72%; (2) Corn straw powder pretreatment The corn stalk powder is placed in a sodium hydroxide solution, the temperature is increased to 80-85°C, and the mixture is stirred for 2.3-2.7 hours. After the stirring is completed, the mixture is filtered out and washed, and the mixture is placed in an ethanol solution, polyethylene glycol 1000 and octadecylamine polyoxyethylene ether are added, and the mixture is stirred evenly at 43-47°C, filtered out and washed to obtain pre-dispersed corn stalk powder. The pre-dispersed corn stalk powder is placed in deionized water, sodium periodate is added, and the mixture is stirred and reacted at 52-57°C for 3.8-4.2 hours. The mixture is filtered, washed, and dried to obtain formaldehyde-modified corn stalk powder. The corn stalk powder has a particle size of 200-220 nm; The mass ratio of the corn stalk powder, sodium hydroxide solution, ethanol solution, polyethylene glycol 1000, and octadecylamine polyoxyethylene ether is 9.8-10.3:98-105:95-106:0.8-1.2:1.1-1.4; The mass concentration of the sodium hydroxide solution is 24-26%; The mass concentration of the ethanol solution is 38-32%; The mass ratio of the pre-dispersed corn stalk powder, deionized water and sodium periodate is 9.0-9.5:96-104:2.4-2.6; (3) Load The taurine is placed in deionized water 1, the temperature is increased to 60-64°C, and after being stirred evenly, the aldehyde-modified corn stalk powder is added, and the reaction is kept warm for 5.8-6.2 hours. After the reaction is completed, it is filtered out and washed, and then put into deionized water 2, and folic acid activation solution is added. The reaction is carried out under a nitrogen atmosphere and at room temperature for 11.7-12.3 hours. After filtering, washing, and drying, a porous loading component is obtained; the porous loading component is put into the plant extract and ultrasonically oscillated. The ultrasonic time is 38-42 minutes, the ultrasonic power is 115-125W, the ultrasonic frequency is 23-27kHz, and the ultrasonic temperature is 48-52°C. After the ultrasonic oscillation is completed, it is filtered and washed, and dried at 60-65°C to constant weight to obtain a functionalized loading material; The mass ratio of taurine, deionized water 1, formaldehyde corn stalk powder, deionized water 2, and folic acid activation solution is 6.0-6.5:116-125:1.6-2.0:110-130:106-115; The preparation method of the folic acid activation liquid is as follows: 2.6-2.8g of folic acid is placed in 95-105g of deionized water, 2.3-2.5g of N-hydroxysuccinimide and 4.0-4.4g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, the pH is adjusted to 5.8-6.2, and the mixture is stirred evenly to obtain the folic acid activation liquid; The mass ratio of the porous support component to the plant extract is 4.8-5.2:52-57; (4) Composite The functionalized load is placed in deionized water, monostearate glyceryl and Tween 20 are added, the temperature is raised to 45-50°C, and the mixture is stirred evenly to obtain a mixed solution 1; gelatin and hydroxyethyl cellulose are placed in deionized water, the temperature is raised to 45-50°C, and the mixture is stirred evenly to obtain a mixed solution 2; the mixed solution 1 is added to the mixed solution 2, and the mixture is stirred at 780-820rpm for 57-64min, and the mixture is spray-dried to obtain a functionalized composite; the functionalized composite is placed in deionized water, protocatechuic aldehyde is added, the temperature is raised to 63-67°C, and the mixture is stirred at 190-210rpm for 5.8-6.2h to obtain a refined feed; The mass ratio of the functionalized load, deionized water, glyceryl monostearate and Tween 20 is 4.8-5.2:37-43:1.8-2.3:1.5-2.0; The mass ratio of gelatin, hydroxyethyl cellulose and deionized water is 3.2-3.6:1.0-1.3:78-82; The mass ratio of the mixed solution 2 to the mixed solution 1 is 5.8-6.2:12.3-12.8; The mass ratio of the functionalized composite, deionized water and protocatechuic aldehyde is 5.2-5.4:78-82:1.3-1.5.

[0008] 2. Adolescence Management Feed the first-phase feed from 12 months of age to the first mating period, 2.3-2.6 kg per cow at 6 am, 2.7-3.2 kg per cow at 12 noon, 2.3-2.7 kg per cow at 5 pm, and 2.4-2.6 kg of alfalfa hay per cow at 10 pm. Feed the second-phase feed from pregnancy to perinatal management. The feeding method is the same as the first-phase feed, and provide sufficient drinking water for the cows, and control the temperature of the cowshed at 20-22℃; The first-phase feed, in parts by weight, includes 26-30 parts of corn stalk silage, 8.0-8.5 parts of royal bamboo grass, 6.0-7.0 parts of corn flour, 3.5-4.0 parts of sorghum flour, 2.8-3.2 parts of carrots, 0.9-1.1 parts of salt, 0.50-0.55 parts of calcium hydrogen phosphate, 0.12-0.16 parts of potassium magnesium sulfate and 0.08-0.12 parts of urea phosphate; The second-phase feed, in parts by weight, includes 45.7-46.2 parts of the first-phase feed and 4.3-4.8 parts of the refined feed.

[0009] 3. Perinatal management Feed the cows with peripartum feed and free drinking water from 20 days before to 20 days after delivery. Feed the cows with peripartum feed four times a day, at 6 am, 12 noon, 5 pm and 10 pm, 2.8-3.2 kg each time. Feed the cows with peripartum fluid at 9 am on the 1st to 5th day after delivery, 1900-2100 g each time. Provide the cows with sufficient drinking water and control the temperature of the cowshed at 22-24℃. The peripartum feed, in parts by weight, includes 28-30 parts of silage corn stalks, 4.6-5.0 parts of sweet potato leaves, 7.0-7.5 parts of refined feed, 8.8-9.2 parts of corn flour, 10.0-10.5 parts of soybean meal, 1.6-1.8 parts of molasses, 1.2-1.5 parts of nutritional elements and 0.9-1.1 parts of salt; The perinatal fluid comprises, by weight, 95-105 parts of water, 0.43-0.47 parts of salt, 5.0-5.4 parts of bran, and 0.42-0.47 parts of calcium carbonate; The nutrient elements, in parts by weight, include 0.28-0.32 parts of soybean oligopeptides, 0.07-0.13 parts of vitamin A, 0.16-0.20 parts of vitamin E, 0.10-0.14 parts of manganese sulfate, 0.16-0.18 parts of zinc sulfate, 0.30-0.32 parts of rumen bypassed methionine, and 0.3-0.36 parts of rumen bypassed lysine.

[0010] 4. Lactation management From the 21st day after giving birth to the dry period, the cows are fed with lactation feed, 2.5-2.9kg per cow at 6am, 3.0-3.4kg per cow at noon, 2.5-2.8kg per cow at 5pm, 2.3-2.6kg per cow at 10pm, and sufficient drinking water is provided for the cows, and the temperature of the cowshed is controlled at 21-23℃; The lactation feed comprises, by weight, 19.2-19.8 parts of silage corn stalks, 6.3-6.7 parts of sweet potato leaves, 6.8-7.2 parts of refined feed, 10.3-10.7 parts of corn flour, 5.8-6.2 parts of soybean meal, 0.40-0.45 parts of rumen bypass fat, 0.8-1.2 parts of salt and 3.4-3.6 parts of nutritional additives; The nutritional supplement comprises, by weight, 0.20-0.24 parts of niacin, 0.6-0.8 parts of calcium carbonate, 0.7-0.9 parts of magnesium oxide, 0.28-0.32 parts of vitamin D and 1.0-1.2 parts of sodium bicarbonate.

[0011] 5. Dry period management Feed dry period feed 20 days before the next delivery, feeding dry period feed four times a day, at 6 am, 12 pm, 5 pm and 10 pm, feeding 2.6-3.0 kg each time, and provide sufficient drinking water for dairy cows, and control the temperature of the cowshed at 21-25℃; The dry lactation feed comprises, by weight, 28-32 parts of silage corn stalks, 10.3-10.7 parts of sweet potato leaves, 3.4-3.6 parts of refined feed, 4.4-4.6 parts of corn flour, 2.8-3.2 parts of soybean meal, 0.7-1.3 parts of salt, 0.48-0.52 parts of calcium hydrogen phosphate, 0.10-0.12 parts of potassium magnesium sulfate and 0.11-0.13 parts of urea phosphate.

[0012] Compared with the prior art, the present invention has achieved the following beneficial effects: 1. The present invention carries out staged feeding in the breeding process of dairy cows, wherein refined feed is fed in the peripartum period and the lactation period. In the process of preparing the refined feed, active ingredients of dandelion, astragalus and tongcao are added, which can improve the immunity and disease resistance of dairy cows. Dandelion can improve mammary gland health, regulate intestinal performance, and improve production performance. Astragalus can also promote milk secretion of dairy cows. Tongcao has a good lactation effect, improves milk quality, and maintains the health of dairy cows. Then, corn stalks are used as a basic carrier, which is first dispersed and oxidized so that the surface contains more aldehyde groups. Taurine and folic acid are added to cross-link the corn stalks, which can not only improve its loading capacity, but also improve the stability of the porous carrier, and can fully load the plant extract, thereby avoiding the reduction of activity of plant active ingredients during feeding and long-term placement, reducing irritation to the gastrointestinal tract of dairy cows, and improving feed utilization. Taurine can improve the immunity of dairy cows. Immunity, promote the development of calves, improve the gastrointestinal absorption performance of dairy cows to promote milk production, improve milk quality, in the compounding process, gelatin is used for secondary coating, and protocatechuic aldehyde is added for reaction, protocatechuic aldehyde cross-links gelatin and functionalized loads to form a stable cross-linking structure, improves the stability of refined feed, enhances the adaptability to the dairy cow matrix, and has a long-term and stable promotion to the milk production capacity of dairy cows, so that the prepared refined feed has a great improvement on the immunity, milk production, milk quality and milk production stability of dairy cows. It is combined with other nutrients in different periods. On the one hand, it improves the health and matrix immunity of dairy cows themselves, enhances anti-stress performance, and on the other hand, ensures the physical development of calves, and improves milk production and milk quality, ensures the milk production stability of dairy cows themselves, so that they still have a high milk production in the middle and late stages of lactation, and improves the milk protein and milk fat content of milk; 2. The breeding method of the present invention has low incidence rates of diarrhea and fever in the adolescent period, peri-lactation period, lactation period and dry period. The number of heads with diarrhea and fever in the adolescent period is 0, the number of heads with diarrhea in the perinatal period is 0-1, and the number of heads with fever is 0-1; the number of heads with diarrhea in the lactation period is 0-1, and the number of heads with fever is 0-1; the number of heads with diarrhea in the dry period is 0-1, and the number of heads with fever is 0; 3. Using the breeding method of the present invention, the weight of the calf born by the dairy cow is 27.1-27.8 kg; 4. Using the breeding method of the present invention, the milk production in the early lactation period is 32.0-32.4 kg / head / day, the milk production in the peak lactation period is 37.4-37.9 kg / head / day, the milk production in the middle lactation period is 34.2-34.8 kg / head / day, and the milk production in the late lactation period is 29.3-29.7 kg / head / day; 5. By adopting the breeding method of the present invention, the milk fat content of the milk is 4.27-4.42%, and the milk protein content is 3.48-3.53%. DETAILED DESCRIPTION

[0013] In order to more clearly understand the technical features, purposes and effects of the present invention, specific embodiments of the present invention are now described.

[0014] Example 1 1. Preparation of refined feed (1) Extraction 5.7g of dandelion, 1.2g of astragalus and 1.5g of tongcao were vacuum freeze-dried, the vacuum degree was controlled to be 0.06MPa, the temperature was -40℃, the freeze-drying time was 48h, after the freeze-drying was completed, after returning to room temperature, crushed through a 120-mesh sieve to obtain a mixed powder; 8 times the mass of ethanol solution was added to the mixed powder for a first ultrasonic extraction, the ultrasonic power was 500W, the ultrasonic time was 50min, the ultrasonic frequency was 40kHz, and the ultrasonic temperature was 47℃. After the first ultrasonic extraction was completed, separation was performed to obtain a first ultrasonic extract and a solid extract; the solid extract was placed in a 10 times the mass of ethanol solution for a second ultrasonic extraction, the ultrasonic power was 520W, the ultrasonic time was 50min, the ultrasonic frequency was 40kHz, and the ultrasonic temperature was 47℃. After the second ultrasonic extraction was completed, separation was performed to obtain a second ultrasonic extract; the first ultrasonic extract and the second ultrasonic extract were mixed, stirred evenly, and concentrated under reduced pressure at 50℃ and 0.07MPa to 50% of the original volume to obtain a plant extract; Therefore, the volume concentration of ethanol solution is 70%; (2) Corn straw powder pretreatment 10.0 g corn stalk powder was placed in 100 g 25 wt% sodium hydroxide solution, the temperature was raised to 83°C, and the mixture was stirred for 2.5 h. After stirring, the mixture was filtered out and washed, and placed in 100 g 30 wt% ethanol solution. 1.0 g polyethylene glycol 1000 and 1.3 g octadecylamine polyoxyethylene ether were added, and the mixture was stirred evenly at 45°C. The mixture was filtered out and washed to obtain pre-dispersed corn stalk powder. 9.3 g pre-dispersed corn stalk powder was placed in 100 g deionized water, 2.5 g sodium periodate was added, and the mixture was stirred at 55°C for 4.0 h. The mixture was filtered, washed, and dried to obtain formaldehyde-modified corn stalk powder. The corn stalk powder has a particle size of 210 nm; (3) Load 6.3 g of taurine was placed in 120 g of deionized water, the temperature was raised to 62°C, and after stirring, 1.8 g of formaldehyded corn stalk powder was added, and the mixture was kept warm for 6.0 h. After the reaction was completed, the mixture was filtered out and washed, and then placed in 120 g of deionized water. 110 g of folic acid activation solution was added, and the mixture was reacted under a nitrogen atmosphere for 12.0 h at room temperature. After filtering, washing, and drying, a porous loading component was obtained. 5.0 g of the porous loading component was placed in 55 g of plant extract and ultrasonically oscillated. The ultrasonic time was 40 min, the ultrasonic power was 120 W, the ultrasonic frequency was 25 kHz, and the ultrasonic temperature was 50°C. After the ultrasonic oscillation was completed, the mixture was filtered, washed, and dried at 62°C to constant weight to obtain a functionalized loading material. The preparation method of the folic acid activation liquid is as follows: 2.7 g of folic acid is placed in 100 g of deionized water, 2.4 g of N-hydroxysuccinimide and 4.2 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, the pH is adjusted to 6.0, and the mixture is stirred evenly to obtain the folic acid activation liquid; (4) Composite Place 5.0g of the functionalized load in 40g of deionized water, add 2.0g of monostearate and 1.7g of Tween 20, heat to 47°C, and stir to obtain a mixed solution 1; place 3.4g of gelatin and 1.1g of hydroxyethyl cellulose in 80g of deionized water, heat to 47°C, and stir to obtain a mixed solution 2; add 12.5g of mixed solution 1 to 6.0g of mixed solution 2, stir at 800rpm for 60min, and spray dry to obtain a functionalized complex; place 5.3g of the functionalized complex in 80g of deionized water, add 1.4g of protocatechuic aldehyde, heat to 65°C, stir at 200rpm for 6.0h, and after the reaction, filter, wash and dry to obtain a refined feed.

[0015] 2. Adolescence Management Feed the first-phase feed from 12 months of age to the first mating period, 2.5 kg per cow at 6 am, 3.0 kg per cow at 12 noon, 2.5 kg per cow at 5 pm, and 2.5 kg of alfalfa hay per cow at 10 pm. Feed the second-phase feed from pregnancy to perinatal management. The feeding method is the same as the first-phase feed, and provide sufficient drinking water for the cows, and control the temperature of the cowshed at 21°C; The first-phase feed, in parts by weight, includes 28 parts of corn stalk silage, 8.2 parts of royal bamboo grass, 6.4 parts of corn flour, 3.8 parts of sorghum flour, 3.0 parts of carrots, 1.0 parts of salt, 0.53 parts of calcium hydrogen phosphate, 0.14 parts of potassium magnesium sulfate and 0.10 parts of urea phosphate; The second-phase feed, in parts by weight, includes 46.0 parts of the first-phase feed and 4.5 parts of the refined feed.

[0016] 3. Perinatal management Feed the dairy cows with peripartum feed and free drinking water from 20 days before to 20 days after delivery. Feed the dairy cows with peripartum feed four times a day, at 6 am, 12 noon, 5 pm and 10 pm, 3.0 kg each time. Feed the dairy cows with peripartum fluid at 9 am on the 1st to 5th day after delivery, 2000 g each time. Provide the dairy cows with sufficient drinking water and control the temperature of the cowshed at 23℃. The perinatal feed, in parts by weight, includes 29 parts of silage corn stalks, 4.8 parts of sweet potato leaves, 7.2 parts of refined feed, 9.0 parts of corn flour, 10.3 parts of soybean meal, 1.7 parts of molasses, 1.4 parts of nutritional elements and 1.0 part of salt; The perinatal fluid comprises, by weight, 100 parts of water, 0.45 parts of salt, 5.2 parts of bran, and 0.45 parts of calcium carbonate; The nutrient elements, in parts by weight, include 0.30 parts of soybean oligopeptides, 0.10 parts of vitamin A, 0.18 parts of vitamin E, 0.12 parts of manganese sulfate, 0.17 parts of zinc sulfate, 0.31 parts of rumen bypassed methionine, and 0.34 parts of rumen bypassed lysine.

[0017] 4. Lactation management From the 21st day after giving birth to the dry period, the cows were fed with lactation feed, 2.7 kg per cow at 6 a.m., 3.2 kg per cow at noon, 2.7 kg per cow at 5 p.m., and 2.5 kg of alfalfa hay at 10 p.m., and sufficient drinking water was provided for the cows, and the temperature of the cowshed was controlled at 22°C; The lactation feed, in parts by weight, comprises 19.5 parts of silage corn stalks, 6.5 parts of sweet potato leaves, 7.0 parts of refined feed, 10.5 parts of corn flour, 6.0 parts of soybean meal, 0.42 parts of rumen bypass fat, 1.0 parts of salt and 3.5 parts of nutritional additives; The nutritional supplement comprises, by weight, 0.22 parts of niacin, 0.7 parts of calcium carbonate, 0.8 parts of magnesium oxide, 0.30 parts of vitamin D and 1.1 parts of sodium bicarbonate.

[0018] 5. Dry period management Feed dry period feed 20 days before the next delivery of dairy cows from the time of weaning to the time of next delivery. Feed dry period feed four times a day, at 6 am, 12 pm, 5 pm and 10 pm, 2.8 kg each time, and provide sufficient drinking water for dairy cows, and control the temperature of the cowshed at 23℃; The dry lactation feed, in parts by weight, includes 30 parts of silage corn stalks, 10.5 parts of sweet potato leaves, 3.5 parts of refined feed, 4.5 parts of corn flour, 3.0 parts of soybean meal, 1.0 part of salt, 0.50 parts of calcium hydrogen phosphate, 0.11 parts of potassium magnesium sulfate and 0.13 parts of urea phosphate.

[0019] Example 2 1. Preparation of refined feed (1) Extraction 5.6g of dandelion, 1.0g of astragalus and 1.3g of tongcao were vacuum freeze-dried, the vacuum degree was controlled to be 0.05MPa, the temperature was -38℃, the freeze-drying time was 46h, after the freeze-drying was completed, after returning to room temperature, crushed through a 110-mesh sieve to obtain a mixed powder; 6 times the mass of ethanol solution was added to the mixed powder for a first ultrasonic extraction, the ultrasonic power was 492W, the ultrasonic time was 47min, the ultrasonic frequency was 37kHz, and the ultrasonic temperature was 45℃. After the first ultrasonic extraction was completed, separation was performed to obtain a first ultrasonic extract and a solid extract; the solid extract was placed in an 8 times the mass of ethanol solution for a second ultrasonic extraction, the ultrasonic power was 510W, the ultrasonic time was 47min, the ultrasonic frequency was 37kHz, and the ultrasonic temperature was 45℃. After the second ultrasonic extraction was completed, separation was performed to obtain a second ultrasonic extract; the first ultrasonic extract and the second ultrasonic extract were mixed, stirred evenly, and concentrated under reduced pressure at 48℃ and 0.06MPa to 48% of the original volume to obtain a plant extract; Therefore, the volume concentration of the ethanol solution is 68%; (2) Corn straw powder pretreatment 9.8 g corn stalk powder was placed in 98 g 24 wt % sodium hydroxide solution, the temperature was raised to 80° C., the mixture was stirred for 2.3 h, after stirring, the mixture was filtered out and washed, the mixture was placed in 95 g 28 wt % ethanol solution, 0.8 g polyethylene glycol 1000 and 1.1 g octadecylamine polyoxyethylene ether were added, the mixture was stirred evenly at 43° C., the mixture was filtered out and washed to obtain pre-dispersed corn stalk powder, 9.0 g pre-dispersed corn stalk powder was placed in 96 g deionized water, 2.4 g sodium periodate was added, the mixture was stirred at 52° C. for 3.8 h, the mixture was filtered, washed and dried to obtain formaldehyded corn stalk powder; The corn stalk powder has a particle size of 200 nm; (3) Load 6.0g of taurine was placed in 116g of deionized water, the temperature was raised to 60°C, and after stirring, 1.6g of formaldehyde corn stalk powder was added, and the reaction was kept warm for 5.8h. After the reaction was completed, it was filtered out and washed, and then put into 110g of deionized water, and 106g of folic acid activation solution was added. The reaction was carried out under a nitrogen atmosphere and at room temperature for 11.7h. After filtering, washing and drying, a porous loading component was obtained; 4.8g of the porous loading component was put into 52g of plant extract and ultrasonically oscillated. The ultrasonic time was 38min, the ultrasonic power was 115W, the ultrasonic frequency was 23kHz, and the ultrasonic temperature was 48°C. After the ultrasonic oscillation was completed, it was filtered and washed, and dried at 60°C to constant weight to obtain a functionalized loading material; The preparation method of the folic acid activation liquid is as follows: 2.6 g of folic acid is placed in 95 g of deionized water, 2.3 g of N-hydroxysuccinimide and 4.0 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, the pH is adjusted to 5.8, and the mixture is stirred evenly to obtain the folic acid activation liquid; (4) Composite Place 4.8g of the functionalized load in 37g of deionized water, add 1.8g of monostearate glyceryl and 1.5g of Tween 20, heat to 45°C, and stir to obtain a mixed solution 1; place 3.2g of gelatin and 1.0g of hydroxyethyl cellulose in 78g of deionized water, heat to 45°C, and stir to obtain a mixed solution 2; add 12.3g of mixed solution 1 to 5.8g of mixed solution 2, stir at 780rpm for 57min, and spray dry to obtain a functionalized complex; place 5.2g of the functionalized complex in 78g of deionized water, add 1.3g of protocatechuic aldehyde, heat to 63°C, stir at 190rpm for 5.8h, and after the reaction, filter, wash and dry to obtain a refined feed.

[0020] 2. Adolescence Management Feed the first-phase feed from 12 months of age to the first mating period, 2.3 kg per cow at 6 am, 2.7 kg per cow at 12 noon, 2.7 kg per cow at 5 pm, and 2.4 kg per cow at 10 pm. Feed the second-phase feed from pregnancy to perinatal management. The feeding method is the same as the first-phase feed, and provide sufficient drinking water for the cows, and control the temperature of the cowshed at 20℃; The first-phase feed, in parts by weight, includes 26 parts of corn stalk silage, 8.0 parts of royal bamboo grass, 7.0 parts of corn flour, 3.5 parts of sorghum flour, 2.8 parts of carrots, 0.9 parts of salt, 0.50 parts of calcium hydrogen phosphate, 0.12 parts of potassium magnesium sulfate and 0.08 parts of urea phosphate; The second-phase feed, in parts by weight, includes 45.7 parts of the first-phase feed and 4.3 parts of the refined feed.

[0021] 3. Perinatal management Feed the dairy cows with peripartum feed and free drinking water from 20 days before to 20 days after delivery. Feed the dairy cows with peripartum feed four times a day, at 6 am, 12 noon, 5 pm and 10 pm, 2.8 kg each time. Feed the dairy cows with peripartum fluid at 9 am on the 1st to 5th day after delivery, 1900 g each time. Provide the dairy cows with sufficient drinking water and control the temperature of the cowshed at 22℃. The perinatal feed, in parts by weight, includes 28 parts of silage corn stalks, 4.6 parts of sweet potato leaves, 7.0 parts of refined feed, 8.8 parts of corn flour, 10.0 parts of soybean meal, 1.6 parts of molasses, 1.2 parts of nutrient elements and 0.9 parts of salt; The perinatal fluid comprises, by weight, 95 parts of water, 0.43 parts of salt, 5.0 parts of bran, and 0.42 parts of calcium carbonate; The nutrient elements, in parts by weight, include 0.28 parts of soybean oligopeptides, 0.07 parts of vitamin A, 0.16 parts of vitamin E, 0.10 parts of manganese sulfate, 0.16 parts of zinc sulfate, 0.30 parts of rumen bypassed methionine, and 0.32 parts of rumen bypassed lysine.

[0022] 4. Lactation management From the 21st day after giving birth to the dry period, the cows were fed with lactation feed, 2.5 kg per cow at 6 am, 3.0 kg per cow at noon, 2.5 kg per cow at 5 pm, and 2.6 kg of alfalfa hay at 10 pm. The cows were also provided with sufficient drinking water and the temperature of the cowshed was controlled at 21°C. The lactation feed, in parts by weight, includes 19.2 parts of silage corn stalks, 6.3 parts of sweet potato leaves, 6.8 parts of refined feed, 10.3 parts of corn flour, 5.8 parts of soybean meal, 0.40 parts of rumen bypass fat, 0.8 parts of salt and 3.4 parts of nutritional additives; The nutritional supplement comprises, by weight, 0.20 parts of niacin, 0.6 parts of calcium carbonate, 0.7 parts of magnesium oxide, 0.28 parts of vitamin D and 1.0 parts of sodium bicarbonate.

[0023] 5. Dry period management Feed dry period feed 20 days before the next delivery of dairy cows from the time of weaning to the time of delivery. Feed dry period feed four times a day, at 6 am, 12 pm, 5 pm and 10 pm, 2.6 kg each time, provide sufficient drinking water for dairy cows, and control the temperature of the cowshed at 21℃. The dry lactation feed, in parts by weight, includes 28 parts of silage corn stalks, 10.3 parts of sweet potato leaves, 3.4 parts of refined feed, 4.4 parts of corn flour, 2.8 parts of soybean meal, 0.7 parts of salt, 0.48 parts of calcium hydrogen phosphate, 0.10 parts of potassium magnesium sulfate and 0.11 parts of urea phosphate.

[0024] Example 3 1. Preparation of refined feed (1) Extraction 6.0g of dandelion, 1.4g of astragalus and 1.7g of tongcao were vacuum freeze-dried, the vacuum degree was controlled to be 0.08MPa, the temperature was -42℃, the freeze-drying time was 50h, after the freeze-drying was completed, after returning to room temperature, crushed through a 130-mesh sieve to obtain a mixed powder; 10 times the mass of ethanol solution was added to the mixed powder for a first ultrasonic extraction, the ultrasonic power was 510W, the ultrasonic time was 53min, the ultrasonic frequency was 43kHz, and the ultrasonic temperature was 50℃. After the first ultrasonic extraction was completed, separation was performed to obtain a first ultrasonic extract and a solid extract; the solid extract was placed in a 12 times the mass of ethanol solution for a second ultrasonic extraction, the ultrasonic power was 530W, the ultrasonic time was 53min, the ultrasonic frequency was 43kHz, and the ultrasonic temperature was 50℃. After the second ultrasonic extraction was completed, separation was performed to obtain a second ultrasonic extract; the first ultrasonic extract and the second ultrasonic extract were mixed, stirred evenly, and concentrated under reduced pressure at 52℃ and 0.08MPa to 52% of the original volume to obtain a plant extract; Therefore, the volume concentration of the ethanol solution is 72%; (2) Corn straw powder pretreatment 10.3 g corn stalk powder was placed in 105 g 26 wt % sodium hydroxide solution, the temperature was raised to 85° C., the mixture was stirred for 2.7 h, after stirring, it was filtered out and washed, placed in 106 g 32 wt % ethanol solution, 1.2 g polyethylene glycol 1000 and 1.4 g octadecylamine polyoxyethylene ether were added, stirred evenly at 47° C., filtered out and washed to obtain pre-dispersed corn stalk powder, 9.5 g pre-dispersed corn stalk powder was placed in 104 g deionized water, 2.6 g sodium periodate was added, stirred and reacted at 57° C. for 4.2 h, filtered, washed and dried to obtain formaldehyded corn stalk powder; The corn stalk powder has a particle size of 220 nm; (3) Load 6.5 g of taurine was placed in 125 g of deionized water, the temperature was raised to 64°C, and after stirring, 2.0 g of aldehyded corn stalk powder was added, and the reaction was kept warm for 6.2 hours. After the reaction was completed, it was filtered out and washed, and then put into 130 g of deionized water, and 115 g of folic acid activation solution was added. The reaction was carried out under a nitrogen atmosphere and at room temperature for 12.3 hours. After filtering, washing, and drying, a porous loading component was obtained; 5.2 g of the porous loading component was put into 57 g of plant extract and ultrasonically oscillated. The ultrasonic time was 42 minutes, the ultrasonic power was 125 W, the ultrasonic frequency was 27 kHz, and the ultrasonic temperature was 52°C. After the ultrasonic oscillation was completed, it was filtered and washed, and dried at 65°C to constant weight to obtain a functionalized loading material; The preparation method of the folic acid activation liquid is as follows: 2.8 g of folic acid is placed in 105 g of deionized water, 2.5 g of N-hydroxysuccinimide and 4.4 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, the pH is adjusted to 6.2, and the mixture is stirred evenly to obtain the folic acid activation liquid; (4) Composite Place 5.2g of functionalized load in 43g of deionized water, add 2.3g of monostearate glyceryl and 2.0g of Tween 20, heat to 50°C, and stir to obtain mixed solution 1; place 3.6g of gelatin and 1.3g of hydroxyethyl cellulose in 82g of deionized water, heat to 50°C, and stir to obtain mixed solution 2; add 12.8g of mixed solution 1 to 6.2g of mixed solution 2, stir at 820rpm for 64min, and spray dry to obtain a functionalized complex; place 5.4g of the functionalized complex in 82g of deionized water, add 1.5g of protocatechuic aldehyde, heat to 67°C, stir at 210rpm for 6.2h, and after the reaction, filter, wash and dry to obtain a refined feed.

[0025] 2. Adolescence Management Feed the first-phase feed from 12 months of age to the first mating period, 2.6 kg per cow at 6 am, 3.2 kg per cow at 12 noon, 2.3 kg per cow at 5 pm, and 2.6 kg of alfalfa hay per cow at 10 pm. Feed the second-phase feed from pregnancy to perinatal management. The feeding method is the same as the first-phase feed, and provide sufficient drinking water for the cows, and control the temperature of the cowshed at 22°C; The first-phase feed, in parts by weight, includes 30 parts of corn stalk silage, 8.5 parts of royal bamboo grass, 6.0 parts of corn flour, 4.0 parts of sorghum flour, 3.2 parts of carrots, 1.1 parts of salt, 0.55 parts of calcium hydrogen phosphate, 0.16 parts of potassium magnesium sulfate and 0.12 parts of urea phosphate; The second-phase feed, in parts by weight, includes 46.2 parts of the first-phase feed and 4.8 parts of the refined feed.

[0026] 3. Perinatal management Feed the cows with peripartum feed and free drinking water from 20 days before to 20 days after delivery. Feed the cows with peripartum feed four times a day, at 6 am, 12 noon, 5 pm and 10 pm, 3.2 kg each time. Feed the cows with peripartum fluid at 9 am on the 1st to 5th day after delivery, 2100 g each time. Provide the cows with sufficient drinking water and control the temperature of the cowshed at 24℃. The perinatal feed, in parts by weight, includes 30 parts of silage corn stalks, 5.0 parts of sweet potato leaves, 7.5 parts of refined feed, 9.2 parts of corn flour, 10.5 parts of soybean meal, 1.8 parts of molasses, 1.5 parts of nutrient elements and 1.1 parts of salt; The perinatal fluid, in parts by weight, includes 105 parts of water, 0.47 parts of salt, 5.4 parts of bran, and 0.47 parts of calcium carbonate; The nutrient elements, in parts by weight, include 0.32 parts of soybean oligopeptides, 0.13 parts of vitamin A, 0.20 parts of vitamin E, 0.14 parts of manganese sulfate, 0.18 parts of zinc sulfate, 0.32 parts of rumen bypassed methionine, and 0.36 parts of rumen bypassed lysine.

[0027] 4. Lactation management From the 21st day after giving birth to the dry period, the cows were fed with lactation feed, 2.9 kg per cow at 6 a.m., 3.4 kg per cow at noon, 2.8 kg per cow at 5 p.m., and 2.3 kg per cow at 10 p.m., and sufficient drinking water was provided for the cows, and the temperature of the cowshed was controlled at 23°C; The lactation feed, in parts by weight, includes 19.8 parts of silage corn stalks, 6.7 parts of sweet potato leaves, 7.2 parts of refined feed, 10.7 parts of corn flour, 6.2 parts of soybean meal, 0.45 parts of rumen bypass fat, 1.2 parts of salt and 3.6 parts of nutritional additives; The nutritional supplement comprises, by weight, 0.24 parts of niacin, 0.8 parts of calcium carbonate, 0.9 parts of magnesium oxide, 0.32 parts of vitamin D and 1.2 parts of sodium bicarbonate.

[0028] 5. Dry period management Feed dry period feed 20 days before the next delivery of dairy cows from the time of weaning to the time of next delivery. Feed dry period feed four times a day, at 6 am, 12 pm, 5 pm and 10 pm, 3.0 kg each time, and provide sufficient drinking water for dairy cows, and control the temperature of the cowshed at 25℃; The dry lactation feed, in parts by weight, includes 32 parts of silage corn stalks, 10.7 parts of sweet potato leaves, 3.6 parts of refined feed, 4.6 parts of corn flour, 3.2 parts of soybean meal, 1.3 parts of salt, 0.52 parts of calcium hydrogen phosphate, 0.12 parts of potassium magnesium sulfate and 0.13 parts of urea phosphate.

[0029] Comparative Example 1 Based on Example 1, the changes are as follows: (1) In the loading step of preparing refined feed, the porous loading component is replaced with pre-dispersed corn stover powder in equal amounts; That is, the loading step is to put 5.0g of pre-dispersed corn stalk powder into 55g of plant extract, and perform ultrasonic oscillation, the ultrasonic time is 40min, the ultrasonic power is 120W, the ultrasonic frequency is 25kHz, and the ultrasonic temperature is 50°C. After the ultrasonic oscillation is completed, it is filtered and washed, and dried at 62°C to constant weight to obtain a functionalized loading material; (2) In the peripartum management steps, nutrient elements were omitted and replaced with corn stalk silage in equal amounts; The rest of the operations are the same.

[0030] Comparative Example 2 Based on Example 1, the changes are as follows: (1) In the loading step of preparing the refined feed, the compounding step is omitted; the functionalized loading material obtained in the loading step is the refined feed; (2) In the lactation management step, nutrient additives were omitted and replaced with silage corn stalks in equal amounts; The rest of the operations are the same.

[0031] Cow feeding 1. Experimental Design 100 Holstein cows of 12 months old with good body condition and similar weight were selected and randomly divided into 5 groups, recorded as experimental groups 1-5, with 20 cows in each group, and then raised according to the following method:

[0032] The rest are managed according to general breeding procedures.

[0033] 2. Disease situation During the experiment, the incidence of diarrhea, fever and other diseases in dairy cows at different times was observed and recorded. The number of cows with diarrhea and fever was recorded. The results are as follows:

[0034] 3. Production situation Record the average weight of calves born to dairy cows as follows:

[0035] 4. Milk production Starting from the time the cows give birth, the average milk production of the cows in each experimental group at different lactation stages was recorded. The early lactation stage was calculated from the time of giving birth to 15 days after giving birth; the peak lactation stage was calculated from 16-90 days after giving birth; the mid-lactation stage was calculated from 91-140 days after giving birth; and the late lactation stage was calculated from 141 days after giving birth to before the dry period. The results are as follows:

[0036] 5. Milk quality The average content of milk fat and milk protein in the milk of each test group of dairy cows during lactation was tested, and the results are as follows:

[0037] The present invention carries out staged feeding in the breeding process of dairy cows, wherein refined feed is fed in the peripartum period and the lactation period. In the process of preparing the refined feed, active ingredients of dandelion, astragalus and tongcao are added, which can improve the immunity and disease resistance of dairy cows. Dandelion can improve mammary gland health, regulate intestinal performance and improve production performance. Astragalus can also promote milk secretion of dairy cows. Tongcao has a good lactation-promoting effect, improves milk quality and maintains the health of dairy cows. Then, corn stalks are used as a basic carrier, which is first dispersed and oxidized to have more aldehyde groups on its surface. Taurine and folic acid are added to cross-link the corn stalks, which can improve its loading capacity and the stability of the porous carrier, and can fully load the plant extract, thereby avoiding the reduction of activity of plant active ingredients during feeding and long-term placement, reducing stimulation to the gastrointestinal tract of dairy cows, and improving feed utilization. In addition, taurine can improve the immunity of dairy cows. The invention relates to the synthesis of milk powder, which is a kind of milk-producing protein, and a kind of milk-producing protein with a certain content of 1g / 10g. It can promote the development of calves, improve the gastrointestinal absorption performance of dairy cows, thereby promoting milk production and improving milk quality. In the compounding process, gelatin is used for secondary coating, and protocatechuic aldehyde is added for reaction. Protocatechuic aldehyde cross-links gelatin and functionalized loads to form a stable cross-linking structure, thereby improving the stability of refined feed, enhancing the adaptability to the matrix of dairy cows, and having a long-term and stable promotion to the milk production capacity of dairy cows, so that the prepared refined feed has a great improvement on the immunity, milk production, milk quality and milk production stability of dairy cows. It is combined with other nutrients in different periods to improve the physical health and matrix immunity of dairy cows and enhance their anti-stress performance on the one hand, and ensure the physical development of calves on the other hand, and improve the milk production and milk quality, and ensure the milk production stability of dairy cows themselves, so that they still have a high milk production in the middle and late stages of lactation, and improve the milk protein and milk fat content of milk.

[0038] In the step of preparing refined feed, the plant extract is directly loaded with pre-dispersed corn stalk powder, which has poor loading capacity for the plant extract and incomplete loading, and the plant extract cannot be fully and stably loaded in the pores of the corn stalk powder. In addition, specific ingredients such as taurine and folic acid are omitted, which reduces the immune performance and milk production performance of dairy cows. In the compounding step, the cross-linking of protocatechuic aldehyde to pre-dispersed corn stalk powder and gelatin is unstable, which affects the effective activity of the refined feed, causes greater gastrointestinal irritation to dairy cows, and has a low feed utilization rate. In addition, the nutrients for the peripartum period are omitted, and nutritional supplements cannot be provided to dairy cows in a timely manner, resulting in the inability to effectively improve the immunity and anti-stress ability of dairy cows, and reducing milk production and milk quality. In the step of preparing refined feed in Comparative Example 2, the compounding step was omitted, and the functionalized load obtained after loading was directly used as refined feed, which had poor stability and unstable structure, which affected the milk production performance and milk production stability of dairy cows, reduced milk production, and reduced the milk protein and milk fat content of milk.

[0039] Unless otherwise specified, all ratios and percentages described in the present invention are by mass ratios and percentages are by mass percentages.

[0040] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A breeding method for increasing milk production of dairy cows, characterized in that: Including preparation of refined feed, management of adolescent period, perinatal period, lactation period and dry period; The preparation of refined feed includes extraction, corn stalk powder pretreatment, loading and compounding steps; The loading step comprises placing taurine in deionized water 1, raising the temperature to 60-64° C., stirring evenly, adding formaldehyded corn stalk powder, and keeping the temperature for reaction for 5.8-6.2 hours. After the reaction is completed, filtering and washing, placing taurine in deionized water 2, adding folic acid activation solution, reacting in a nitrogen atmosphere, reacting at room temperature for 11.7-12.3 hours, filtering, washing, and drying to obtain a porous loading component; placing the porous loading component in a plant extract, performing ultrasonic oscillation, filtering, washing, and drying at 60-65° C. to constant weight after the ultrasonic oscillation is completed to obtain a functionalized loading substance; The compounding step comprises placing the functionalized load in deionized water, adding glyceryl monostearate and Tween 20, heating to 45-50° C., and stirring to obtain a mixed solution 1; placing gelatin and hydroxyethyl cellulose in deionized water, heating to 45-50° C., and stirring to obtain a mixed solution 2; adding the mixed solution 1 to the mixed solution 2, stirring at 780-820 rpm for 57-64 min, and spray drying to obtain a functionalized composite; placing the functionalized composite in deionized water, adding protocatechuic aldehyde, heating to 63-67° C., stirring at 190-210 rpm for reaction for 5.8-6.2 h, and filtering, washing, and drying after the reaction to obtain a refined feed; The peripartum management step includes daily feeding of peripartum feed; the peripartum feed includes silage corn stalks, sweet potato leaves, refined feed, corn flour, soybean meal, molasses, nutrients and salt; The nutrient elements include soybean oligopeptides, vitamin A, vitamin E, manganese sulfate, zinc sulfate, rumen bypassed methionine, and rumen bypassed lysine; The lactation management step includes daily feeding of lactation feed, wherein the lactation feed includes silage corn stalks, sweet potato leaves, refined feed, corn flour, soybean meal, rumen bypass fat, salt and nutritional additives; The nutritional additives include niacin, calcium carbonate, magnesium oxide, vitamin D and sodium bicarbonate.

2. A breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The extraction step comprises: performing vacuum freeze drying on dandelion, astragalus and tongcao, controlling the vacuum degree to be 0.05-0.08MPa, the temperature to be -42--38°C, the freeze drying time to be 46-50h, and after the freeze drying is completed, returning to room temperature, crushing through a 110-130 mesh sieve to obtain a mixed powder; adding 6-10 times the mass of ethanol solution to the mixed powder for one ultrasonic extraction, the ultrasonic power to be 492-510W, the ultrasonic time to be 47-53min, the ultrasonic frequency to be 37-43kHz, the ultrasonic temperature to be 45-50°C, and the one ultrasonic extraction to be completed. After that, separate to obtain a primary ultrasonic extract and a solid extract; place the solid extract in an ethanol solution of 8-12 times the mass for secondary ultrasonic extraction, the ultrasonic power is 510-530W, the ultrasonic time is 47-53min, the ultrasonic frequency is 37-43kHz, the ultrasonic temperature is 45-50°C, after the secondary ultrasonic extraction is completed, separate to obtain a secondary ultrasonic extract; mix the primary ultrasonic extract and the secondary ultrasonic extract, stir evenly, and then reduce the pressure and concentrate to 48-52% of the original volume at 48-52°C and 0.06-0.08MPa to obtain a plant extract; The mass ratio of dandelion, astragalus and tongcao is 5.6-6.0:1.0-1.4:1.3-1.7; Therefore, the volume concentration of the ethanol solution is 68-72%.

3. A breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The corn stalk powder pretreatment step comprises placing the corn stalk powder in a sodium hydroxide solution, raising the temperature to 80-85° C., keeping the temperature and stirring for 2.3-2.7 hours, filtering out and washing after the stirring is completed, placing the corn stalk powder in an ethanol solution, adding polyethylene glycol 1000 and octadecylamine polyoxyethylene ether, stirring evenly at 43-47° C., filtering out and washing to obtain pre-dispersed corn stalk powder, placing the pre-dispersed corn stalk powder in deionized water, adding sodium periodate, stirring and reacting at 52-57° C. for 3.8-4.2 hours, filtering, washing and drying to obtain formaldehyded corn stalk powder; The corn stalk powder has a particle size of 200-220 nm; The mass ratio of the corn stalk powder, sodium hydroxide solution, ethanol solution, polyethylene glycol 1000, and octadecylamine polyoxyethylene ether is 9.8-10.3:98-105:95-106:0.8-1.2:1.1-1.4; The mass concentration of the sodium hydroxide solution is 24-26%; The mass concentration of the ethanol solution is 38-32%; The mass ratio of the pre-dispersed corn stalk powder, deionized water and sodium periodate is 9.0-9.5:96-104:2.4-2.

6.

4. The method for increasing the milk production of dairy cows according to claim 1, characterized in that: In the loading step, the ultrasonic oscillation, the ultrasonic time is 38-42 minutes, the ultrasonic power is 115-125W, the ultrasonic frequency is 23-27kHz, and the ultrasonic temperature is 48-52°C; The mass ratio of taurine, deionized water 1, formaldehyde corn stalk powder, deionized water 2, and folic acid activation solution is 6.0-6.5:116-125:1.6-2.0:110-130:106-115; The preparation method of the folic acid activation liquid is as follows: 2.6-2.8g of folic acid is placed in 95-105g of deionized water, 2.3-2.5g of N-hydroxysuccinimide and 4.0-4.4g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride are added, the pH is adjusted to 5.8-6.2, and the mixture is stirred evenly to obtain the folic acid activation liquid; The mass ratio of the porous load component to the plant extract is 4.8-5.2:52-57.

5. The method for increasing the milk production of dairy cows according to claim 1, characterized in that: In the compounding step, the mass ratio of the functionalized load, deionized water, glyceryl monostearate and Tween 20 is 4.8-5.2:37-43:1.8-2.3:1.5-2.0; The mass ratio of gelatin, hydroxyethyl cellulose and deionized water is 3.2-3.6:1.0-1.3:78-82; The mass ratio of the mixed solution 2 to the mixed solution 1 is 5.8-6.2:12.3-12.8; The mass ratio of the functionalized composite, deionized water and protocatechuic aldehyde is 5.2-5.4:78-82:1.3-1.

5.

6. The breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The youth management step is to feed the first-phase feed from 12 months of age to the first mating period, feed 2.3-2.6 kg of feed to each cow at 6 o'clock in the morning, feed 2.7-3.2 kg of feed to each cow at 12 o'clock in the afternoon, feed 2.3-2.7 kg of feed to each cow at 5 o'clock in the afternoon, and feed 2.4-2.6 kg of alfalfa hay to each cow at 10 o'clock in the evening. Before the cows are pregnant to the perinatal management, they are fed with the second-phase feed, and the feeding method is the same as the feeding method of the first-phase feed, and sufficient drinking water is provided for the cows, and the temperature of the cowshed is controlled at 20-22°C; The first-phase feed, in parts by weight, includes 26-30 parts of corn stalk silage, 8.0-8.5 parts of royal bamboo grass, 6.0-7.0 parts of corn flour, 3.5-4.0 parts of sorghum flour, 2.8-3.2 parts of carrots, 0.9-1.1 parts of salt, 0.50-0.55 parts of calcium hydrogen phosphate, 0.12-0.16 parts of potassium magnesium sulfate and 0.08-0.12 parts of urea phosphate; The second-phase feed, in parts by weight, includes 45.7-46.2 parts of the first-phase feed and 4.3-4.8 parts of the refined feed.

7. The breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The peripartum management steps are as follows: feeding the dairy cows with peripartum feed and free drinking water from 20 days before to 20 days after delivery, feeding the dairy cows with peripartum feed four times a day, at 6 a.m., 12 noon, 5 p.m. and 10 p.m., feeding 2.8-3.2 kg each time, feeding peripartum fluid at 9 a.m. every day on the 1st to 5th day after delivery, feeding 1900-2100 g each time, providing the dairy cows with sufficient drinking water, and controlling the temperature of the cowshed to be 22-24° C.; The peripartum feed, in parts by weight, includes 28-30 parts of silage corn stalks, 4.6-5.0 parts of sweet potato leaves, 7.0-7.5 parts of refined feed, 8.8-9.2 parts of corn flour, 10.0-10.5 parts of soybean meal, 1.6-1.8 parts of molasses, 1.2-1.5 parts of nutritional elements and 0.9-1.1 parts of salt; The perinatal fluid comprises, by weight, 95-105 parts of water, 0.43-0.47 parts of salt, 5.0-5.4 parts of bran, and 0.42-0.47 parts of calcium carbonate; The nutrient elements, in parts by weight, include 0.28-0.32 parts of soybean oligopeptides, 0.07-0.13 parts of vitamin A, 0.16-0.20 parts of vitamin E, 0.10-0.14 parts of manganese sulfate, 0.16-0.18 parts of zinc sulfate, 0.30-0.32 parts of rumen bypassed methionine, and 0.3-0.36 parts of rumen bypassed lysine.

8. The breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The lactation management step is to feed the lactation feed from the 21st day after the cows give birth to the dry period, feed each cow 2.5-2.9 kg of feed at 6 am, feed each cow 3.0-3.4 kg of feed at 12 noon, feed each cow 2.5-2.8 kg of feed at 5 pm, feed each cow 2.3-2.6 kg of alfalfa hay at 10 pm, provide the cows with sufficient drinking water, and control the temperature of the cowshed to be 21-23° C.; The lactation feed comprises, by weight, 19.2-19.8 parts of silage corn stalks, 6.3-6.7 parts of sweet potato leaves, 6.8-7.2 parts of refined feed, 10.3-10.7 parts of corn flour, 5.8-6.2 parts of soybean meal, 0.40-0.45 parts of rumen bypass fat, 0.8-1.2 parts of salt and 3.4-3.6 parts of nutritional additives; The nutritional supplement comprises, by weight, 0.20-0.24 parts of niacin, 0.6-0.8 parts of calcium carbonate, 0.7-0.9 parts of magnesium oxide, 0.28-0.32 parts of vitamin D and 1.0-1.2 parts of sodium bicarbonate.

9. The breeding method for increasing milk production of dairy cows according to claim 1, characterized in that: The dry period management step is to feed the cows dry period feed 20 days before the next delivery from the time when the cows stop lactating to the time when the cows give birth, feed the cows dry period feed four times a day, at 6 am, 12 pm, 5 pm and 10 pm, feed 2.6-3.0 kg each time, provide the cows with sufficient drinking water, and control the temperature of the cowshed to be 21-25°C; The dry lactation feed comprises, by weight, 28-32 parts of silage corn stalks, 10.3-10.7 parts of sweet potato leaves, 3.4-3.6 parts of refined feed, 4.4-4.6 parts of corn flour, 2.8-3.2 parts of soybean meal, 0.7-1.3 parts of salt, 0.48-0.52 parts of calcium hydrogen phosphate, 0.10-0.12 parts of potassium magnesium sulfate and 0.11-0.13 parts of urea phosphate.

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