Plant composition for regulating scalp micro-ecology and application thereof

Abstract

The invention discloses a plant composition for regulating scalp micro-ecology and application thereof. The plant composition is mainly prepared from the following raw materials: Fuding white tea extract, pepper extract, viola mandshurica extract / butterfly bean flower extract and oat peptide extract in a mass ratio of (15-30): (15-30): (15-30): (10-25). When the plant composition disclosed by the invention is applied to a washing and caring product, the washing and caring product contains 4.5-15% of the plant composition disclosed by the invention, 12-24% of a surfactant, 0.5-1.5% of a hair conditioner, 0.5-1.5% of a humectant and the balance of water, wherein the total percentage is 100%. According to the invention, four plant raw materials with respective effects are scientifically compounded, so that various effective components play a synergistic effect, and the scalp micro-ecology can be regulated, scalp flora balance can be rebuilt, and a scalp barrier function can be enhanced, so that the effects of effectively inhibiting bacteria and maintaining scalp health can be achieved.

Description

1. Technical field:

[0001] The present invention belongs to the technical field of traditional Chinese medicines and also to the technical field of toiletries, and in particular relates to a plant composition for regulating scalp microecology and an application thereof. 2. Background technology:

[0002] Skin microecology is a microecological system composed of microorganisms, skin surface tissues, various secretions and microenvironment. Its physiological functions mainly include nutrition, self-purification, barrier, immunity and participation in skin tissue metabolism. The balance of skin microecology is jointly maintained by skin microecology, the host and the external environment, and is closely related to skin health.

[0003] Compared with the skin on other parts of the human body, the scalp is a unique ecological niche. 3 ~10 5 CFU / mm 2 Microbial cells, including bacteria, fungi and viruses, are mainly found on the surface of the scalp and in skin appendages such as hair follicles and sebaceous glands. These microorganisms and the host form a complex scalp microecology. The destruction of the scalp microbial balance will change the scalp's physiological environment and damage the scalp's barrier function. When the scalp barrier function is damaged, on the one hand, external irritants can more easily penetrate into the stratum corneum, causing inflammatory reactions and abnormal desquamation of the scalp. On the other hand, changes in scalp pH, antimicrobial peptide secretion, etc. caused by barrier damage may cause changes in the composition and activity of scalp microorganisms, and ultimately manifest as scalp sensitivity or lesions. Therefore, a comprehensive, multi-angle, scientific and well-founded conditioning and maintenance of the scalp is necessary to maintain a healthy scalp.

[0004] In terms of scalp health management, plant resources such as traditional Chinese medicine have natural advantages. With the increase in market demand, the use of natural ingredients from traditional Chinese medicine plant resources to regulate the balance of scalp microecology has gradually become a research hotspot, providing new ideas for the development of scalp care products that regulate scalp microecology. Building scalp oil-water balance, improving scalp barrier function, improving hair nutrition, reducing dandruff, itching, reducing hair loss and improving damage are important means of scalp microecological health care.

[0005] Fuding white tea is one of the six major types of tea in my country. It is named because it is mainly produced in Fuding and other places. Its production process is unique, without frying or kneading, and the process is natural and simple. The fresh leaves are withered and dried in two simple processes, which is natural and unique in terms of nutrients. Modern research shows that the flavonoid content and amino acid content in Fuding white tea are generally higher than other teas. It has good effects of clearing heat and removing fire, anti-allergy, anti-oxidation, anti-radiation, and delaying aging. It is widely regarded as a good medicine for treating and caring for measles patients in North China and Fujian. Zhou Lianggong of the Qing Dynasty wrote in "Min Xiaoji": "Baihao Yinzhen, produced in Hongxue Cave, Taimu Mountain, is cold in nature and has the same effect as rhinoceros horn. It is a holy medicine for treating measles." Simonetti (2017) showed that plant extracts rich in flavonoids and polyphenol compounds have the effect of inhibiting the activity of Malassezia furfur, and can fight against Malassezia globosa by acting on the target Malassezia β-carbonic anhydrase, thereby achieving the effect of removing dandruff; plant extracts have a significant effect on removing dandruff and can effectively relieve itching. Therefore, the development and application of plant compositions such as Fuding white tea in scalp microecological care has important theoretical and practical significance. Invention patent application CN201611116033.8 discloses a white tea extract and its preparation method and application. The technical scheme involves a white tea extract with multiple functions such as moisturizing, whitening, nourishing, and anti-aging. The invention patent ZL2022102403143 discloses a whitening and antibacterial cosmetic containing Fuding white tea extract and its preparation method. However, there is no report on its scalp microecological care.

[0006] Zanthoxyli Pericarpium is a Chinese herbal medicine that is both edible and medicinal. According to traditional Chinese medicine, Zanthoxyli Pericarpium has the functions of warming the middle and relieving pain, killing insects, detoxifying, and relieving itching. It is mainly used to treat abdominal pain caused by cold, vomiting and diarrhea caused by cold and dampness, abdominal pain caused by worm accumulation, eczema itching, and vaginal itching in women. The invention patent application CN202210162403.0 discloses a preparation process for a scalp essence containing Zanthoxyli Pericarpium peel extract.

[0007] Northeast Viola (Viola mandshurica W.Beck.) is the whole herb of the Violaceae family, also known as Viola mandshurica and Viola yedoensis. Functions and indications: clearing away heat and detoxifying, cooling blood and reducing swelling. It is mainly used to treat carbuncle swelling, carbuncle back, red eyes and sore throat, etc. It has a long history of use and was first recorded in Qianjin Fang. It was recorded in the Compendium of Materia Medica by Li Shizhen of the Ming Dynasty. It is bitter, pungent and cold, and can cure all carbuncle back. The Chinese Pharmacopoeia records that it mainly treats clearing heat and detoxifying, cooling blood and reducing swelling. It is currently mainly used in the fields of food and medicine, and is less used in the field of cosmetics. Modern pharmacological studies have shown that Northeast Viola has antibacterial, anti-inflammatory, antioxidant, antiviral, anticoagulant and other effects. Coumarin, flavonoids, lignans, terpenes, steroids, alkaloids and other chemical components have been found in this plant, especially rich in flavonoid compounds that are beneficial to the skin, which provides a basis for its application in cosmetics.

[0008] Butterfly pea (Clitoria ternatea) is a genus of the leguminous family Clitoria, also known as blue butterfly, butterfly flower bean, and blue flower bean. It is widely planted in tropical and subtropical regions around the world. Butterfly pea has a wide range of uses and has potential application value in modern medicine. Butterfly pea flower extract contains active substances such as flavonoids, alkaloids, tannins, saponins and phenols, and has antibacterial, anti-inflammatory, antioxidant and other effects. The invention patent application of CN114948814A discloses the application of Butterfly pea flower extract in skin care products, but there is no report on its scalp microecology and hair care efficacy.

[0009] Plants are a natural treasure trove of bioactive peptides. Plant-derived bioactive peptides are rich in variety and novel in structure, and have been a research hotspot in recent years. Bioactive peptides have antioxidant, antibacterial, and health-care functions. Sosalagere (2022) reported that oats are rich in antioxidant peptides and have good free radical scavenging activity; plant-derived bioactive peptides also have biological activities such as promoting tissue healing, regulating immunity in the body, and regulating skin microecological balance. Therefore, the development of plant-derived bioactive peptides is of great significance to the development of food, medicine, and beauty. The invention patent CN115054534A discloses a method for preparing oat peptides and shampoo mousse containing oat peptides from oat bran. 3. Summary of the invention:

[0010] The technical problem to be solved by the present invention is: based on the current application status of plant resources such as traditional Chinese medicine in scalp health management, the present invention provides a plant composition for regulating scalp microecology and its application. The plant composition of the present invention can be used to prepare daily chemical products and has the function of regulating the balance of scalp microecology. The technical scheme of the present invention is not only to simply inhibit and kill harmful bacteria through antibacterial agents, but also to create a healthy microecological environment for the scalp and hair roots through the synergistic functions of functional ingredients such as antibacterial, antioxidant, anti-allergic, anti-inflammatory and promotion of the growth of beneficial bacteria in the skin, thereby effectively improving the nutritional status and oil-water balance of the scalp and improving the barrier function of the scalp.

[0011] In order to solve the above problems, the technical solution adopted by the present invention is:

[0012] The invention provides a plant composition for regulating scalp microecology. The plant composition mainly consists of raw materials: Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract / Cephala ternata flower extract and oat peptide extract, and the mass ratio of the four is 15-30:15-30:15-30:10-25.

[0013] According to the above-mentioned plant composition for regulating scalp microecology, the plant composition is mainly composed of raw materials Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio between the four is 18-30:18-30:18-30:15-20.

[0014] According to the above-mentioned plant composition for regulating scalp microecology, the plant composition is mainly composed of the raw materials Fuding white tea extract, Zanthoxylum bungeanum extract, Manchurian violet extract and oat peptide extract, and the mass ratio between the four is 20-25:20-25:20-25:16-20.

[0015] According to the above-mentioned plant composition for regulating scalp microecology, the plant composition is mainly composed of raw materials Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio between the four is 24-28:24-28:24-28:16-18.

[0016] According to the above-mentioned plant composition for regulating scalp microecology, the preparation method of the Fuding white tea extract is: placing the Fuding white tea in an extraction tank, and then adding ethanol with a volume fraction of 30-75% for ultrasonic extraction, the extraction temperature is 40-65°C, the extraction time is 60-90min, the mass volume ratio of the Fuding white tea and ethanol added is 1g:20-50mL, and ultrasonic extraction is performed 2-3 times under the same conditions; the obtained extracts are combined, filtered and concentrated to a paste-like crude extract after the combination to obtain the Fuding white tea extract;

[0017] The preparation method of the pepper extract is as follows: crushing pepper into pepper powder, then adding 50-75% ethanol in an amount of 3-30 times the weight of the pepper powder for ultrasonic extraction, the extraction temperature is 40-70° C., the extraction time is 1-8 hours, the obtained extract is filtered and concentrated under reduced pressure in turn, and the pepper extract is obtained;

[0018] The preparation method of the northeastern violet extract is as follows: placing the northeastern violet in an extraction tank, then adding ethanol with a volume fraction of 70-90% for ultrasonic extraction, the extraction temperature is 45-65° C., the extraction time is 30-60 min, and the mass volume ratio of the northeastern violet to the ethanol is 1 g: 20-40 mL; ultrasonic extraction is performed 3 times under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the northeastern violet extract;

[0019] The preparation method of the butterfly pea flower extract is as follows: placing butterfly pea flowers in an extraction tank, then adding ethanol with a volume fraction of 55-75% for ultrasonic extraction, the extraction temperature is 40-60° C., the extraction time is 40-60 min, and the mass volume ratio of the butterfly pea flowers and the ethanol is 1 g: 30-50 mL; ultrasonic extraction is performed 2-3 times under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the butterfly pea flower extract;

[0020] The preparation method of the oat peptide extract is as follows: weigh oat seeds and grind them, put them into a conical flask after grinding, use acetone to defatted oat flour, discard the supernatant, dry in a fume hood and volatilize the solvent, and collect the oat flour; add 0.1 mol / L Tris-HCl buffer with pH 8.8 to the obtained oat flour according to the mass volume ratio of 1g:12-30mL, and then extract at 30-40°C for 40-90min; then centrifuge at 4°C and 5000r / min for 15min to obtain the supernatant; add an equal volume of 12% trichloroacetic acid to the obtained supernatant to mix, let it stand for 30min after mixing, and centrifuge at 4°C and 10000r / min for 15min to precipitate macromolecular proteins after standing, and the obtained supernatant is the oat peptide extract.

[0021] In addition, a method for preparing a hair care product using the plant composition for regulating scalp microecology is provided.

[0022] According to the application of the above-mentioned plant composition in the preparation of hair care products, when preparing the hair care products, the raw material composition of the hair care products contains the plant composition for regulating scalp microecology as claimed in claim 1.

[0023] According to the application of the above-mentioned plant composition in the preparation of a hair care product, expressed in terms of mass percentage, the hair care product contains 4.5-15% of the plant composition for regulating scalp microecology according to claim 1, 12-24% of a surfactant, 0.5-1.5% of a hair conditioner, 0.5-1.5% of a moisturizer, and water is added to 100%.

[0024] According to the use of the above-mentioned plant composition in the preparation of washing and care products, the surfactant is at least one of ammonium laureth sulfate, sodium laureth-6 carboxylate, ammonium lauryl sulfate, sodium lauroyl glutamate, sodium cocoyl glutamate and sodium cocoylaminopropionate;

[0025] The hair conditioning agent is at least one of alanine, proline, threonine, serine, biotin, hydrogenated castor oil, wheat protein and olive oil;

[0026] The moisturizing agent is at least one of glycerin, allantoin, arginine, betaine and phytosphingosine.

[0027] The positive beneficial effects of the present invention are:

[0028] 1. The present invention provides a plant composition that can be used to regulate the microecology of the scalp. The plant composition is a scientific compound of four plant raw materials with different functions, namely, Fuding white tea, prickly ash, northeastern violet / butterfly pea flower and oat peptide, using the traditional Chinese medicine compatibility principle, so that the four plant raw materials can give full play to the synergistic effect, which is conducive to improving the efficacy and activity of the plant composition in terms of antibacterial, antioxidant, anti-allergic, anti-dandruff and antipruritic effects; effectively inhibiting harmful bacteria, promoting the growth of beneficial bacteria, regulating the microecology of the scalp, rebuilding the balance of the scalp flora, enhancing the barrier function of the scalp, and maintaining the health of the scalp.

[0029] 2. The test examples of the present invention show that the plant composition of the present invention can effectively regulate the balance of scalp microecology, and has a significant regulating effect on the important members of the skin microecology, namely Malassezia furfur, Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis; it can effectively inhibit the growth and reproduction of scalp harmful bacteria, namely Propionibacterium furfur, Malassezia furfur and Staphylococcus aureus; and promote the growth of scalp beneficial bacteria, namely Staphylococcus epidermidis, so that it enters the logarithmic growth phase and the stable phase earlier. In addition, the plant composition of the present invention also has significant antioxidant capacity and anti-allergic effects: the plant composition containing the northeastern violet extract has a DPPH free radical scavenging rate of up to 91.02%, a superoxide anion free radical scavenging rate of up to 90.73%, a hydroxyl free radical scavenging rate of up to 97.36%, and a hyaluronidase inhibition rate of 85.12%; the plant composition containing the butterfly pea flower extract has a DPPH free radical scavenging rate of up to 93.08%, a superoxide anion free radical scavenging rate of up to 94.42%, a hydroxyl free radical scavenging rate of up to 99.11%, and a hyaluronidase inhibition rate of 86.86%.

[0030] 3. The preparation method of the plant composition for regulating scalp microecology provided by the present invention is simple and easy to operate, and the effective parts of the raw materials are completely extracted. The application examples of the present invention in the preparation of washing and care products show that the shampoo prepared using the plant composition provided by the present invention has good stability, and will not be stratified or deteriorated after long-term storage; and it has rich foam, mild nature, high safety, no irritation to the eyes and skin, and will not cause allergic reactions; the test volunteers are highly satisfied with the assessment indicators such as soothing, antipruritic, dandruff removal, and improvement of scalp oil-water balance. Long-term use can enhance the immunity of the skin and effectively solve the problem of imbalance of the head skin microecology. 4. Description of the drawings:

[0031] Figure 1 The effect diagram of the plant composition of the present invention (containing the Viola manchuriana extract) on the inhibition of hyaluronidase;

[0032] Figure 2 A graph showing the effect of the plant composition of the present invention (containing the extract of Viola manchuria) on the growth curve of Staphylococcus epidermidis;

[0033] Figure 3 The effect diagram of the inhibitory effect of the plant composition of the present invention (containing butterfly pea flower extract) on hyaluronidase;

[0034] Figure 4 A graph showing the effect of the plant composition of the present invention (containing butterfly pea flower extract) on the growth trend of Staphylococcus epidermidis. V. Specific implementation methods:

[0035] The following further describes the plant composition (containing the Viola mandshurica extract) for regulating scalp microecology of the present invention in combination with Examples 1-6, but does not limit the scope of protection of the technical solution of the present invention.

[0036] Embodiment 1:

[0037] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 15:30:30:25.

[0038] The preparation method of the Fuding white tea extract is as follows: placing the Fuding white tea in an extraction tank, then adding 50% by volume ethanol for ultrasonic extraction, the extraction temperature is 50° C., the extraction time is 75 min, and the mass volume ratio of the Fuding white tea and the ethanol added is 1 g:20 mL; ultrasonic extraction is performed 3 times under the same conditions, the obtained extracts are combined, and after the combination, they are filtered and concentrated to a paste-like crude extract to obtain the Fuding white tea extract.

[0039] The preparation method of the pepper extract is as follows: crushing the pepper into pepper powder, then adding 75% ethanol in an amount 15 times the weight of the pepper powder for ultrasonic extraction, the extraction temperature is 50° C., the extraction time is 1 hour, the obtained extract is filtered, and concentrated under reduced pressure to obtain the pepper extract.

[0040] The preparation method of the Viola manchuria extract is as follows: placing the Viola manchuria in an extraction tank, then adding ethanol with a volume fraction of 75% for ultrasonic extraction, the extraction temperature is 65°C, the extraction time is 30 minutes, and the mass volume ratio of the Viola manchuria to the ethanol is 1g:20mL; ultrasonic extraction is performed 3 times under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the Viola manchuria extract.

[0041] The preparation method of the oat peptide extract is as follows: weigh oat seeds and grind them, put them into a conical flask after grinding, use acetone to defatted oat powder, discard the supernatant, dry in a fume hood and volatilize the solvent, and collect the oat powder; add 0.1 mol / L Tris-HCl buffer solution with pH 8.8 to the obtained oat powder according to a mass volume ratio of 1g:15mL, extract at 30°C for 60min; then centrifuge at 4°C and 5000r / min for 15min to obtain the supernatant; add an equal volume of 12% trichloroacetic acid to the obtained supernatant to mix, let stand for 30min after mixing, and centrifuge at 4°C and 10000r / min for 15min to precipitate macromolecular proteins, and the obtained supernatant is the oat peptide extract.

[0042] Embodiment 2:

[0043] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 18:20:22:20.

[0044] The preparation method of the Fuding white tea extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 55%, the mass volume ratio of Fuding white tea to ethanol is 1 g:24 mL, the extraction temperature is 55° C., and the extraction time is 80 min.

[0045] The preparation method of the Zanthoxylum bungeanum extract is substantially the same as that of Example 1, except that the volume fraction of ethanol is 70%, the weight of ethanol added is 18 times the weight of Zanthoxylum bungeanum powder, the extraction temperature is 45° C., and the extraction time is 1.5 h.

[0046] The preparation method of the Viola manchuria extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 70%, the mass volume ratio of Viola manchuria to ethanol is 1 g:24 mL, the extraction temperature is 48° C., and the extraction time is 35 min.

[0047] The preparation method of the oat peptide extract is basically the same as that of Example 1, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:18 mL, and extraction is performed at 35° C. for 65 min.

[0048] Embodiment 3:

[0049] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 20:18:21:21.

[0050] The preparation method of the Fuding white tea extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 60%, the mass volume ratio of the Fuding white tea and ethanol added is 1g:30mL, the extraction temperature is 60°C, and the extraction time is 85min.

[0051] The preparation method of the Zanthoxylum bungeanum extract is substantially the same as that of Example 1, except that the ethanol is 60% ethanol, the weight of the ethanol added is 20 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 65° C., and the extraction time is 1.8 h.

[0052] The preparation method of the Viola manchuria extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 80%, the mass volume ratio of Viola manchuria to ethanol is 1 g:30 mL, the extraction temperature is 45° C., and the extraction time is 50 min.

[0053] The preparation method of the oat peptide extract is basically the same as that of Example 1, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:20 mL, and extraction is performed at 40° C. for 70 min.

[0054] Embodiment 4:

[0055] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 22:24:20:24.

[0056] The preparation method of the Fuding white tea extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 65%, the mass volume ratio of the Fuding white tea and ethanol added is 1g:35mL, the extraction temperature is 65°C, and the extraction time is 90min.

[0057] The preparation method of the Zanthoxylum bungeanum extract is substantially the same as that of Example 1, except that the ethanol is 70% ethanol, the weight of the added ethanol is 24 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 55° C., and the extraction time is 2 h.

[0058] The preparation method of the Viola manchuria extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 85%, the mass volume ratio of Viola manchuria to ethanol is 1 g:35 mL, the extraction temperature is 50° C., and the extraction time is 60 min.

[0059] The preparation method of the oat peptide extract is basically the same as that of Example 1, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:24 mL, and extraction is performed at 30° C. for 75 min.

[0060] Embodiment 5:

[0061] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 20:22:24:18.

[0062] The preparation method of the Fuding white tea extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 70%, the mass volume ratio of the Fuding white tea and ethanol added is 1g:40mL, the extraction temperature is 55°C, and the extraction time is 75min.

[0063] The preparation method of the Zanthoxylum bungeanum extract is substantially the same as that of Example 1, except that the ethanol is 65% ethanol, the weight of the ethanol added is 30 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 60° C., and the extraction time is 2.5 h.

[0064] The preparation method of the Viola manchuria extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 90%, the mass volume ratio of Viola manchuria to ethanol is 1 g:40 mL, the extraction temperature is 60° C., and the extraction time is 55 min.

[0065] The preparation method of the oat peptide extract is basically the same as that of Example 1, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:30 mL, and extraction is performed at 38° C. for 80 min.

[0066] Embodiment 6:

[0067] The plant composition for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Manchurian violet extract and oat peptide extract, and the mass ratio of the four is 25:25:25:18.

[0068] The preparation method of the Fuding white tea extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 75%, the mass volume ratio of the Fuding white tea and ethanol added is 1g:45mL, the extraction temperature is 50°C, and the extraction time is 75min.

[0069] The preparation method of the Zanthoxylum bungeanum extract is substantially the same as that of Example 1, except that the ethanol is 50% ethanol, the weight of the ethanol added is 25 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 70° C., and the extraction time is 3 h.

[0070] The preparation method of the Viola manchuria extract is basically the same as that of Example 1, except that the volume fraction of ethanol is 75%, the mass volume ratio of Viola manchuria to ethanol is 1 g:25 mL, the extraction temperature is 55° C., and the extraction time is 45 min.

[0071] The preparation method of the oat peptide extract is basically the same as that of Example 1, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:28 mL, and extraction is performed at 36° C. for 90 min.

[0072] The specific examples of the use of the plant composition for regulating scalp microecology (containing the Viola mandshurica extract) of the present invention in the preparation of cleaning products are as follows:

[0073] Application Example 1:

[0074] The plant composition washing and care product for regulating scalp microecology of the present invention is expressed in percentage by mass, comprising 8% of the plant composition of the present invention, 12% of a surfactant, 0.8% of a hair conditioner, 1.5% of a moisturizer, and water added to 100%.

[0075] The surfactant is sodium laureth-6 carboxylate.

[0076] The hair conditioning agent is a composition of alanine, biotin, hydrogenated castor oil, wheat protein and olive oil, and the mass ratio thereof is 2:2:2:5:5.

[0077] The moisturizing agent is a composition of glycerin and allantoin, and the weight ratio thereof is 10:1.

[0078] Application Example 2:

[0079] The plant composition washing and care product for regulating scalp microecology of the present invention is expressed in percentage by mass, comprising 10% of the plant composition of the present invention, 15% of a surfactant, 1.2% of a hair conditioner, 1.2% of a moisturizer, and water added to 100%.

[0080] The surfactant is a composition of ammonium lauryl polyether sulfate and ammonium lauryl sulfate, and the weight ratio thereof is 4:1.

[0081] The hair conditioning agent is a composition of proline, threonine, biotin, hydrogenated castor oil and wheat protein, and the mass ratio thereof is 2:2:2:1:1.

[0082] The moisturizing agent is a composition of glycerol, arginine and betaine, and the weight ratio thereof is 8:1:1.

[0083] Application Example 3:

[0084] The plant composition washing and care product for regulating scalp microecology of the present invention is expressed in percentage by mass, comprising 12% of the plant composition of the present invention, 18% of a surfactant, 1.0% of a hair conditioner, 1.0% of a moisturizer, and water added to 100%.

[0085] The surfactant is a composition of sodium lauroyl glutamate and sodium cocoyl glutamate, and the mass ratio thereof is 1:1.

[0086] The hair conditioning agent is a composition of alanine, serine, biotin and wheat protein, and the mass ratio thereof is 1:2:1:4.

[0087] The moisturizing agent is a composition of allantoin, betaine and phytosphingosine, and the weight ratio thereof is 1:1:1.

[0088] Application Example 4:

[0089] The plant composition washing and care product for regulating scalp microecology of the present invention is expressed in percentage by mass, comprising 12.5% ​​of the plant composition of the present invention, 21% of a surfactant, 0.5% of a hair conditioner, 0.9% of a moisturizer, and water added to 100%.

[0090] The surfactant is a composition of ammonium laureth sulfate, sodium laureth-6 carboxylate and ammonium lauryl sulfate, and the weight ratio thereof is 1:1:2.

[0091] The hair conditioning agent is a composition of proline, threonine, serine and olive oil, and the mass ratio thereof is 2:2:1:5.

[0092] The moisturizing agent is a composition of glycerin, allantoin, arginine and betaine, and the weight ratio thereof is 4:1:1:1.

[0093] Application Example 5:

[0094] The plant composition washing and care product for regulating scalp microecology of the present invention is expressed in percentage by mass, comprising 15% of the plant composition of the present invention, 24% of a surfactant, 0.9% of a hair conditioner, 1.3% of a moisturizer, and water added to 100%.

[0095] The surfactant is a composition of sodium laureth-6 carboxylate, sodium cocoyl glutamate and sodium cocoyl amino propionate, and the weight ratio thereof is 1:1:1.

[0096] The hair conditioning agent is a composition of alanine, biotin, wheat protein and olive oil, and the mass ratio thereof is 1:1:4:4.

[0097] The moisturizing agent is a composition of allantoin, arginine, betaine and phytosphingosine, and the weight ratio thereof is 1:2:2:1.

[0098] The relevant properties of the plant composition of the present invention (containing the Viola manchuria extract) and the toiletries prepared using the plant composition are tested as follows:

[0099] 1) Test Example 1: Evaluation of Antioxidant Capacity

[0100] Antioxidant capacity is listed as one of the main research and development directions by cosmetics companies and is also one of the most important functional demands of the market. Therefore, the total antioxidant capacity, superoxide anion free radical scavenging capacity, and hydroxyl free radical scavenging capacity of the plant compositions of Examples 1 to 6 were tested using the corresponding free radical scavenging capacity test kits, and the corresponding free radical scavenging rates were calculated. The relevant test data obtained are detailed in Tables 1 to 3.

[0101] Table 1 DPPH free radical scavenging ability evaluation (scavenging rate %)

[0102]

[0103] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level. The same below.

[0104] Table 2 Evaluation of superoxide anion scavenging ability (scavenging rate %)

[0105]

[0106] Table 3 Evaluation of hydroxyl radical scavenging ability (scavenging rate %)

[0107]

[0108] It can be seen from the experimental data in Tables 1 to 3 that the plant compositions of Examples 1 to 6 of the present invention have significant total antioxidant capacity, superoxide anion free radical scavenging capacity, and hydroxyl free radical scavenging capacity; and there is a significant dose effect, and the antioxidant capacity of the plant compositions of Examples 1 to 6 is significantly enhanced with increasing concentration. The DPPH free radical scavenging rate is as high as 91.02%, the superoxide anion free radical scavenging rate is as high as 90.73%, and the hydroxyl free radical scavenging rate is as high as 97.36%. It shows that the plant composition for regulating scalp microecology provided by the present invention has significant antioxidant capacity.

[0109] 2) Test Example 2: Evaluation of anti-allergic efficacy

[0110] Allergic reaction is also referred to as skin allergy, which is the immunogenic reaction of the skin to chemical substances or bioactive substances, and can be manifested as pruritus, erythema, papules or blisters. Studies have shown that hyaluronidase is a hyaluronic acid-specific lyase, and its overactivity can lead to the degradation of skin hyaluronic acid, causing damage to the skin barrier, and then causing skin inflammation and allergy; Many antiallergic drugs have a strong inhibitory effect on hyaluronidase activity. Therefore, hyaluronidase inhibition experiment is the most typical in vitro anti-allergic activity evaluation method, i.e., the anti-allergic activity of the material is evaluated with hyaluronidase inhibition rate as an index, and the larger the hyaluronidase inhibition rate, the stronger the anti-allergic activity. Therefore, the present invention adopts the anti-allergic efficacy of the plant composition of the present invention (containing northeastern Viola extract) (randomly selected embodiment 1, embodiment 3 and embodiment 5) evaluated by hyaluronidase inhibition test.

[0111] Hyaluronidase inhibition test: Elson-Morgan method was used. Take a certain amount of CaCl2 solution and hyaluronidase solution and incubate at 37℃ for 20 minutes; add an appropriate amount of sample solution and incubate at 37℃ for 20 minutes; add sodium hyaluronate solution and incubate at 37℃ for 30 minutes, and place it at room temperature for 5 minutes; add NaOH solution and acetylacetone solution, heat it in a boiling water bath for 60 minutes, then immediately cool it for 5 minutes, add P-DAB color developer and dilute it with 96% ethanol, place it for 20 minutes to develop color, and measure the absorbance near 530nm. Ascorbic acid was used as a positive control.

[0112] The inhibitory activity was calculated as follows:

[0113] Hyaluronidase inhibition rate = [(CD)-(AB)] / (CD) × 100%;

[0114] Where:

[0115] A represents the absorbance of the (hyaluronidase + sample + sodium hyaluronate) sample solution;

[0116] B represents the absorbance of the blank solution of (acetate buffer + sample + acetate buffer);

[0117] C represents the absorbance of the (hyaluronidase + deionized water + sodium hyaluronate) control solution;

[0118] D represents the absorbance of the blank solution (acetate buffer + deionized water + acetate buffer).

[0119] The results of the test on the inhibitory effect of the plant composition of the present invention (containing the extract of Viola manchuria) on hyaluronidase are detailed in the attached Figure 1 .

[0120] Depend on Figure 1 It can be seen that the plant composition of the present invention (containing the extract of Viola mandshurica) has a significant inhibitory effect on the activity of hyaluronidase in the mass concentration range of 0.4 to 2.4 mg / mL, and the inhibitory effect has a significant dose effect. The inhibitory effect of Example 1, Example 3 and Example 5 on hyaluronidase is significantly enhanced with increasing dose; at 2.4 mg / mL, the hyaluronidase inhibition rate is as high as 85.12%; however, at the same concentration, the hyaluronic acid inhibition rate of the plant composition of the present invention (containing the extract of Viola mandshurica) is slightly lower than that of the positive control ascorbic acid.

[0121] 3) Experimental Example 3: Regulatory Effect on Skin Microorganisms

[0122] Skin microorganisms are important members of the skin microecology. The human scalp flora can be divided into resident bacteria and transient bacteria. Resident bacteria are microorganisms that settle on healthy skin and are closely related to skin health, mainly including Staphylococcus, Propionibacterium and Malassezia. Transient bacteria are microorganisms that are infected through contact with the external environment, mainly including Staphylococcus aureus, etc. They are the main pathogens that cause skin infections.

[0123] In order to verify the regulatory effect of the plant composition for regulating scalp microecology of the present invention on scalp microorganisms, firstly, Example 2, Example 4, and Example 6 were randomly selected to carry out an effect test on the growth of Staphylococcus epidermidis.

[0124] Effect test on the growth of Staphylococcus epidermidis: Weigh 0.512 g of the plant composition of the present invention (containing the extract of Viola manchuria) and dissolve it in 2 mL of sterile water. Filter it with a 0.22 μm filter membrane and use it as the sample mother solution. Inoculate Staphylococcus epidermidis on an LB plate and culture it at 37°C for 24 hours. Pick a single colony and culture it in LB broth overnight. Adjust A 600nm 0.8~1.0, dilute 1000 times for later use. Add 100μL LB into the 96-well plate, then add 100μL samples with a mass concentration of 4 and 16μg / mL, and set up a blank group at the same time. Place the 96-well plate in a UV / visible full-wavelength microplate reader and measure the absorbance at a wavelength of 600nm. With the incubation time as the horizontal axis, A 600 nm As the vertical axis, draw the growth curve of Staphylococcus epidermidis and analyze the growth trend of Staphylococcus epidermidis (see the attached Figure 2 ).

[0125] Depend on Figure 2 It can be seen that the plant composition of the present invention (containing the Northeast Viola extract) shows a significant promoting effect on the growth of Staphylococcus epidermidis at a mass concentration of 4 to 16 μg / mL, causing Staphylococcus epidermidis to enter the logarithmic growth phase and the stable phase early, and its promoting effect has a significant concentration dependence. When the mass concentration of the plant composition of the present invention (containing the Northeast Viola extract) is 16 μg / mL, the growth-promoting effect on Staphylococcus epidermidis is most ideal. Staphylococcus epidermidis, as a resident bacterium on the skin, has a potential defense function. It can reduce pathogens on the surface of the skin, effectively maintain the community structure on the surface of the skin, and play an important role in skin protection. The experimental results show that the plant composition of the present invention (containing the Northeast Viola extract) can effectively promote the growth of scalp probiotics, which is beneficial to regulating the health of the scalp microecology.

[0126] In order to verify the inhibitory effect of the plant composition of the present invention (containing the extract of Viola manchuria) on harmful bacteria of the scalp, the antibacterial effect test of Examples 1-6 on Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus was carried out.

[0127] Antibacterial test method:

[0128] Culture medium: 2 g / L beef extract, 1.5 g / L peptone, 0.5 g / L L-cystine, 15 g / L agar (not added to liquid culture medium), sterilize at 121°C for 15 min. After sterilization, take out the culture bottles and test tubes and tighten the lids in time to reduce the entry of air into the culture medium.

[0129] Preparation of Propionibacterium acnes suspension: After anaerobic culture for 48 hours, small (0.5-1.5 mm in diameter), round, white or off-white, translucent or opaque, smooth and non-hemolytic colonies are formed. Pick a ring of the bacterial lawn grown on the slope or culture dish with an inoculation needle, inoculate it into the liquid culture medium, and place it in a 37°C constant temperature incubator for anaerobic culture for 48-72 hours to obtain Propionibacterium acnes suspension.

[0130] Preparation of Malassezia furfur suspension: The bacteria grow on the culture medium of the culture dish and show white and slightly yellow colonies with smooth and moist appearance. Pick a ring of the bacterial lawn grown on the slope or the culture dish with an inoculation needle and inoculate it into the liquid culture medium. Seal the mouth of the test tube and shake it on a shaker at 37°C for 24 to 36 hours to obtain the Malassezia furfur suspension.

[0131] Recovery of Staphylococcus aureus strains and preparation of bacterial suspension:

[0132] In a sterile liquid culture medium (100 mL), a small amount of activated bacteria was inoculated with a sterile inoculation loop, fixed in a 37°C constant temperature shaker, and cultured for 24 h to prepare a concentration of 10 6 ~10 8 CFU / mL of bacterial suspension is reserved.

[0133] Preparation of the test solution: After sterilizing the samples of the plant composition cleaning products prepared in Examples 1 to 6 of the present invention, 1 volume of distilled water was added to prepare the test solution.

[0134] Preparation of sterile filter paper: Use a hole puncher to punch holes in the three-layer folded filter paper to make 9 mm round filter paper sheets, and dry-sterilize them at 121°C for 20 min for later use.

[0135] Antibacterial experiment (filter paper inhibition zone method):

[0136] Inject 2mL of Propionibacterium acnes / Malassezia furfur into the culture medium of the culture dish cooled to about 50℃, mix evenly, pour into the culture dish (about 20mL / culture dish), and let it stand horizontally until solidified. Then place the sterile filter paper evenly on the culture medium of the culture dish, 4 filter paper pieces per culture dish, and use a pipette to draw 20μL of the sample to be tested and drop it on the filter paper. Perform three sets of parallel experiments. Then place the Propionibacterium acnes / Malassezia furfur culture dish in an anaerobic tank for culture; place the Staphylococcus aureus culture dish in a constant temperature box (37±1℃) for culture. After 48~72h / 24~36h / 24h, observe the changes in the inhibition zone, measure the diameter of the inhibition zone (mm) with a vernier caliper (with the outer edge of the inhibition zone with no colony growth as the boundary), and record the diameter of the inhibition zone.

[0137] In the antibacterial experiment, on the one hand, the test bacteria begin to grow, and on the other hand, the sample to be tested spreads around the filter paper as the origin, forming a circular area where the colony cannot grow, which is the "inhibition zone". The larger the inhibition zone, the better the antibacterial effect. Criteria for judging the antibacterial effect: if the diameter of the inhibition zone is greater than 15mm, it means that the microorganism is highly sensitive to the antibacterial agent, 10-15mm is moderately sensitive, 6-10mm is lowly sensitive, and less than 6mm is insensitive.

[0138] The above antibacterial experimental results are shown in Table 4.

[0139] Table 4 Antibacterial effect of the plant compositions (1.0 mg / mL) of Examples 1 to 6 of the present invention

[0140]

[0141] As can be seen from the results in Table 4, the plant compositions of Examples 1 to 6 of the present invention have significant inhibitory effects on Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus. According to the inhibition zone judgment standard, when the concentration is 1.0 mg / mL, the diameter of the inhibition zone is greater than 15 mm. It can be seen that the plant composition provided by the present invention can effectively inhibit the growth and reproduction of scalp resident bacteria Propionibacterium acnes and Malassezia furfur, as well as transient bacteria Staphylococcus aureus, that is, the plant composition of the present invention (containing the Northeast Viola extract) can effectively regulate the scalp microecological balance.

[0142] 4) Test Example 4: Safety Evaluation

[0143] In order to verify the safety of the plant composition shampoo product for regulating scalp microecology of the present invention, a safety evaluation test was conducted on the shampoos prepared in Application Examples 1 to 5.

[0144] Skin and eye irritation test:

[0145] The shampoos prepared in Examples 1 to 5 were subjected to acute skin irritation tests and acute eye irritation tests, respectively. The specific experimental methods, scoring criteria and irritation reaction classifications are all in accordance with the 2015 edition of the Hygienic Standards for Cosmetics.

[0146] The skin irritation reaction results show that: no erythema and no edema occur when using the shampoos prepared by Application Examples 1 to 5, and the skin irritation level is non-irritating.

[0147] The eye irritation reaction results showed that: the conjunctiva of the shampoo prepared by using Examples 1 to 5 had no congestion, no edema, and no secretion; the cornea had no turbidity; the iris was normal, and the eye irritation level was non-irritating.

[0148] The above results show that the shampoo formula of the present invention is mild in nature and non-irritating to the skin and eyes.

[0149] Allergy test (human skin patch test):

[0150] The shampoos prepared in Examples 1 to 5 of the present invention were tested with reference to the relevant requirements and determination methods of the "Human Skin Patch Test" in the 2015 edition of the "Cosmetic Hygiene Standards", and the skin reactions were observed at 0h, 6h, 12h, 24h, and 48h, respectively. The experimental results of all volunteer subjects were negative, and no adverse skin reactions occurred. This shows that the shampoo provided by the present invention does not cause allergic reactions, is non-irritating, and has high safety.

[0151] 5) Test Example 5: Evaluation of the use effect

[0152] In order to verify the use effect evaluation of the plant composition shampoo product for regulating scalp microecology of the present invention, the user experience evaluation of the shampoo prepared by Application Examples 1 to 5 was carried out.

[0153] 100 volunteers with sub-healthy scalp (50 males and 50 females, aged 18 to 50 years old) were selected and randomly divided into 10 groups, with 10 people in each group.

[0154] Usage: Each volunteer uses 20-100 mL of shampoo each time according to the thickness of the hair, massages the head for 5 minutes, and then washes it clean. Shampoo once every other day for two consecutive months. Then, the shampoo score is calculated based on the following indicators: soothing, anti-dandruff, anti-itching, oil-water balance, foam abundance, and easy rinsing. The score is based on a 10-point system, with the highest score being 10 and the lowest score being 0. The average score of each volunteer is taken. The results are detailed in Table 5.

[0155] Table 5 User experience evaluation

[0156] Group Soothing Relieve itching Anti-dandruff Oil-water balance Foam abundance Easy to rinse Overall rating Application Example 1 9.8 9.7 8.6 9.1 9.7 9.6 9.4 Application Example 2 9.7 9.6 8.5 9.0 9.6 9.5 9.3 Application Example 3 9.8 9.7 8.6 9.1 9.7 9.6 9.4 Application Example 4 9.6 9.5 8.4 8.9 9.5 9.4 9.2 Application Example 5 9.7 9.6 8.5 9.0 9.8 9.5 9.4

[0157] It can be seen from the experimental data in Table 5 that the shampoo prepared with the plant composition for regulating scalp microecology of the present invention has rich foam and is easy to rinse; the shampoo prepared by the present invention can effectively remove dandruff, improve scalp itching, and effectively solve the problem of scalp oil-water balance. The test volunteers are highly satisfied with the above-mentioned assessment indicators.

[0158] 6) Test Example 6: Stability Test

[0159] In order to verify the stability of the plant composition shampoo product for regulating scalp microecology of the present invention, a stability test was conducted on the shampoos prepared in Example 1 to 5.

[0160] Test method: The shampoos prepared in Examples 1 to 5 were placed at 25°C, -15°C, 45°C and 50°C for 8 weeks, and the products were observed for stratification and water seepage. The specific results are shown in Table 6.

[0161] The judgment criteria are: √ represents no abnormality; ★ represents slight abnormality but acceptable; × represents obvious abnormality and is judged as failing.

[0162] Table 6 Stability test at 25℃, -15℃, 45℃ and 50℃

[0163]

[0164] It can be seen from the test data in Table 6 that the shampoos prepared by Application Examples 1 to 5 of the present invention are exposed to 25°C, -15°C, 45°C and 50°C for 8 weeks without any abnormality and can be used normally, indicating that the shampoos prepared by using the plant composition provided by the present invention have good stability and will not delaminate or deteriorate after long-term storage.

[0165] In summary, the preparation method of the plant composition for regulating scalp microecology containing northeastern violet extract of the present invention is simple and easy to operate, and the effective parts of the raw materials are completely extracted. After the four kinds of plant raw materials with different effects in the plant composition for regulating scalp microecology of the present invention are compounded, a synergistic effect is exerted, and the important members of the skin microecology, Malassezia furfur, Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis, have a significant regulating effect; applied to the formula of daily chemical products for hair use, dandruff can be effectively removed, the problem of scalp itching can be improved, and the oil-water balance of the scalp and the balance of the skin microecology can be adjusted.

[0166] The following further describes the plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention in conjunction with Examples 7-12, but does not limit the scope of protection of the technical solution of the present invention.

[0167] Embodiment 7:

[0168] The plant composition for regulating scalp microecology (containing butterfly pea flower extract) of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 18:18:18:20.

[0169] The preparation method of the Fuding white tea extract is as follows: placing the Fuding white tea in an extraction tank, then adding 30% by volume ethanol for ultrasonic extraction, the extraction temperature is 50° C., the extraction time is 60 min, and the mass volume ratio of the Fuding white tea and the ethanol added is 1 g:40 mL; ultrasonic extraction is performed 3 times under the same conditions, the obtained extracts are combined, and after the combination, they are filtered and concentrated to a paste-like crude extract to obtain the Fuding white tea extract.

[0170] The preparation method of the pepper extract is as follows: crushing the pepper, then adding 65% ethanol in a volume fraction and 4.2 times the weight of the pepper powder for ultrasonic extraction, the extraction temperature is 40° C., the extraction time is 8 hours, the obtained extract is filtered, and concentrated under reduced pressure to obtain the pepper extract.

[0171] The preparation method of the butterfly pea flower extract is as follows: placing the butterfly pea flower in an extraction tank, then adding ethanol with a volume fraction of 55% for ultrasonic extraction, the extraction temperature is 60°C, the extraction time is 40 minutes, and the mass volume ratio of the butterfly pea flower and the ethanol added is 1g:30mL; ultrasonic extraction is performed twice under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the butterfly pea flower extract.

[0172] The preparation method of the oat peptide extract is as follows: weigh oat seeds and crush them, put the obtained oat powder into a conical flask, use acetone to defatt the oat powder, discard the supernatant, dry and volatilize the solvent in a fume hood, and collect the oat powder; add 0.1 mol / L Tris-HCl buffer solution with pH 8.8 to the obtained oat powder according to a mass volume ratio of 1g:12mL, extract at 30°C for 60min; then centrifuge at 4°C and 5000r / min for 15min to obtain the supernatant; add an equal volume of 12% trichloroacetic acid to the obtained supernatant to mix, let stand for 30min after mixing, centrifuge at 4°C and 10000r / min for 15min to precipitate macromolecular proteins, and the obtained supernatant is the oat peptide extract.

[0173] Embodiment 8:

[0174] The plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 18:22:18:20.

[0175] The preparation method of the Fuding white tea extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 50%, the extraction temperature is 48° C., the extraction time is 70 min, and the mass volume ratio of the Fuding white tea and ethanol added is 1 g:30 mL.

[0176] The preparation method of the Zanthoxylum bungeanum extract is basically the same as that of Example 7, except that the volume fraction of the ethanol is 60%, the weight of the added ethanol is 3 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 45° C., and the extraction time is 6 hours.

[0177] The preparation method of the butterfly pea flower extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 75%, the extraction temperature is 40° C., the extraction time is 60 min, the mass volume ratio of the butterfly pea flower and ethanol added is 1 g:35 mL; and ultrasonic extraction is performed 3 times.

[0178] The preparation method of the oat peptide extract is basically the same as that of Example 7, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:20 mL, and extraction is performed at 34° C. for 40 min.

[0179] Embodiment 9:

[0180] The plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 20:24:28:18.

[0181] The preparation method of the Fuding white tea extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 36%, the extraction temperature is 42° C., the extraction time is 80 min, the mass volume ratio of the Fuding white tea and ethanol added is 1 g:35 mL; and ultrasonic extraction is performed twice.

[0182] The preparation method of the Zanthoxylum bungeanum extract is basically the same as that of Example 7, except that the volume fraction of the ethanol is 70%, the weight of the added ethanol is 4 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 50° C., and the extraction time is 7.5 h.

[0183] The preparation method of the butterfly pea flower extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 60%, the extraction temperature is 52° C., the extraction time is 55 min, and the mass volume ratio of the butterfly pea flower and ethanol added is 1 g:38 mL.

[0184] The preparation method of the oat peptide extract is basically the same as that of Example 7, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:15 mL, and extraction is performed at 35° C. for 44 min.

[0185] Embodiment 10:

[0186] The plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 22:22:28:19.

[0187] The preparation method of the Fuding white tea extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 40%, the extraction temperature is 46° C., the extraction time is 65 min, and the mass volume ratio of the Fuding white tea and ethanol added is 1 g:50 mL.

[0188] The preparation method of the Zanthoxylum bungeanum extract is basically the same as that of Example 7, except that the volume fraction of the ethanol is 75%, the weight of the added ethanol is 3.5 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 42° C., and the extraction time is 6.2 h.

[0189] The preparation method of the butterfly pea flower extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 70%, the extraction temperature is 45° C., the extraction time is 45 min, the mass volume ratio of the butterfly pea flower and ethanol added is 1 g:50 mL; and ultrasonic extraction is performed 3 times.

[0190] The preparation method of the oat peptide extract is basically the same as that of Example 7, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:16 mL, and extraction is performed at 36° C. for 55 min.

[0191] Embodiment 11:

[0192] The plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 24:25:26:18.

[0193] The preparation method of the Fuding white tea extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 48%, the extraction temperature is 45° C., the extraction time is 72 min, and the mass volume ratio of the Fuding white tea and ethanol added is 1 g:48 mL.

[0194] The preparation method of the Zanthoxylum bungeanum extract is basically the same as that of Example 7, except that the volume fraction of the ethanol is 65%, the weight of the added ethanol is 5 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 48° C., and the extraction time is 7 h.

[0195] The preparation method of the butterfly pea flower extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 68%, the extraction temperature is 50° C., the extraction time is 50 min, and the mass volume ratio of the butterfly pea flower and ethanol added is 1 g:45 mL.

[0196] The preparation method of the oat peptide extract is basically the same as that of Example 7, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:18 mL, and extraction is performed at 38° C. for 50 min.

[0197] Embodiment 12:

[0198] The plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention is composed of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 20:22:24:16.

[0199] The preparation method of the Fuding white tea extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 46%, the extraction temperature is 44° C., the extraction time is 75 min, and the mass volume ratio of the Fuding white tea and ethanol added is 1 g:46 mL.

[0200] The preparation method of the Zanthoxylum bungeanum extract is basically the same as that of Example 7, except that the volume fraction of the ethanol is 70%, the weight of the added ethanol is 4.5 times the weight of the Zanthoxylum bungeanum powder, the extraction temperature is 46° C., and the extraction time is 6.5 h.

[0201] The preparation method of the butterfly pea flower extract is basically the same as that of Example 7, except that the volume fraction of ethanol is 65%, the extraction temperature is 48° C., the extraction time is 48 min, the mass volume ratio of the butterfly pea flower and ethanol added is 1 g:42 mL; and ultrasonic extraction is performed 3 times.

[0202] The preparation method of the oat peptide extract is basically the same as that of Example 7, except that: 0.1 mol / L Tris-HCl buffer with a pH of 8.8 is added at a mass volume ratio of 1 g:17 mL, and extraction is performed at 32° C. for 52 min.

[0203] The specific examples of the application of the plant composition (containing butterfly pea flower extract) for regulating scalp microecology of the present invention in the preparation of cleaning products are as follows:

[0204] Application Example 6:

[0205] The plant composition washing and care product containing butterfly pea flower extract of the present invention is expressed in percentage by mass, comprising 4.5% of the plant composition of the present invention, 18% of a surfactant, 0.8% of a hair conditioner, 1.5% of a moisturizer, and water added to 100%.

[0206] The surfactant is a composition of ammonium laureth sulfate, sodium laureth-6 carboxylate and ammonium lauryl sulfate, and the weight ratio thereof is 1:1:1.

[0207] The hair conditioning agent is a composition of alanine, wheat protein and olive oil, and the mass ratio thereof is 1:4:4.

[0208] The moisturizing agent is a composition of glycerin and allantoin, and the weight ratio thereof is 12:1.

[0209] Application Example 7:

[0210] The plant composition washing and conditioning product containing butterfly pea flower extract of the present invention is expressed in percentage by mass, comprising 5.5% of the plant composition of the present invention, 16% of a surfactant, 0.9% of a hair conditioner, 1.2% of a moisturizer, and water added to 100%.

[0211] The surfactant is a composition of ammonium laureth sulfate, ammonium lauryl sulfate and sodium cocoyl glutamate, and the weight ratio thereof is 3:1:1.

[0212] The hair conditioning agent is a composition of proline, threonine, biotin and wheat protein, and the mass ratio thereof is 2:2:1:6.

[0213] The moisturizing agent is a composition of glycerol, arginine and phytosphingosine, and the weight ratio thereof is 8:2:1.

[0214] Application Example 8:

[0215] The plant composition washing and care product containing butterfly pea flower extract of the present invention is expressed in percentage by mass, comprising 10% of the plant composition of the present invention, 15% of a surfactant, 1.5% of a hair conditioner, 1.0% of a moisturizer, and water added to 100%.

[0216] The surfactant is a composition of sodium cocoyl glutamate and sodium cocoyl amino propionate, and the weight ratio thereof is 1:1.

[0217] The hair conditioning agent is a composition of alanine, serine and wheat protein, and the mass ratio thereof is 1:1:3.

[0218] The moisturizing agent is a composition of allantoin, betaine and phytosphingosine, and the weight ratio thereof is 2:2:1.

[0219] Application Example 9:

[0220] The plant composition washing and conditioning product containing butterfly pea flower extract of the present invention is expressed in percentage by mass, comprising 7.5% of the plant composition of the present invention, 21% of a surfactant, 1.2% of a hair conditioner, 0.9% of a moisturizer, and water added to 100%.

[0221] The surfactant is a composition of ammonium laureth sulfate, ammonium lauryl sulfate, sodium cocoyl glutamate and sodium cocoyl amino propionate, and the weight ratio thereof is 1:1:1:1.

[0222] The hair conditioning agent is a composition of proline, threonine, serine and hydrogenated castor oil, and the mass ratio of the composition is 1:1:1:1.

[0223] The moisturizing agent is a composition of glycerin, allantoin, arginine and betaine, and the weight ratio thereof is 2:1:1:1.

[0224] Application Example 10:

[0225] The plant composition washing and care product containing butterfly pea flower extract of the present invention is expressed in percentage by mass, comprising 12.5% ​​of the plant composition of the present invention, 24% of a surfactant, 1.0% of a hair conditioner, 0.5% of a moisturizer, and water added to 100%.

[0226] The surfactant is a composition of sodium laureth-6 carboxylate and sodium cocoyl glutamate, with a weight ratio of 2:1.

[0227] The hair conditioning agent is a composition of alanine and olive oil, and the mass ratio thereof is 2:7.

[0228] The moisturizing agent is a composition of allantoin, arginine and phytosphingosine, and the weight ratio thereof is 2:2:1.

[0229] The relevant properties of the plant composition of the present invention (containing butterfly pea flower extract) and the washing products prepared using the plant composition are tested as follows:

[0230] 1) Test Example 1: Evaluation of Antioxidant Capacity

[0231] The total antioxidant capacity, superoxide anion free radical scavenging capacity, and hydroxyl free radical scavenging capacity of the plant compositions of Examples 7 to 12 are detailed in Tables 7 to 9.

[0232] Table 7 DPPH free radical scavenging ability evaluation (scavenging rate %)

[0233]

[0234] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level. The same below.

[0235] Table 8 Evaluation of superoxide anion scavenging ability (scavenging rate %)

[0236]

[0237] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level. The same below.

[0238] Table 9 Evaluation of hydroxyl radical scavenging ability (scavenging rate %)

[0239]

[0240] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level. The same below.

[0241] It can be seen from the experimental data of Tables 7 to 9 that the plant compositions of Examples 7 to 12 of the present invention have significant total antioxidant capacity, superoxide anion free radical scavenging capacity, and hydroxyl free radical scavenging capacity; and there is a significant dose effect, and the antioxidant capacity of the plant compositions of Examples 7 to 12 of the present invention is significantly enhanced with increasing concentration. The DPPH free radical scavenging rate is as high as 93.08%, the superoxide anion free radical scavenging rate is as high as 94.42% or more, and the hydroxyl free radical scavenging rate is as high as 99.11%. It is shown that the plant composition containing butterfly pea flower extract provided by the present invention has significant antioxidant capacity.

[0242] 2) Test Example 2: Evaluation of anti-allergic efficacy

[0243] The anti-allergic efficacy of randomly selected plant compositions of the present invention (containing butterfly pea flower extract) in Example 8, Example 10 and Example 12 was evaluated by using the hyaluronidase inhibition test. The test results are detailed in the attached Figure 3 .

[0244] Depend on Figure 3 It can be seen that the plant composition of the present invention has a significant inhibitory effect on the activity of hyaluronidase within the mass concentration range of 0.4 to 2.4 mg / mL, and the inhibitory effect has a significant dose effect. The inhibitory effect of Example 8, Example 10 and Example 12 on hyaluronidase is significantly enhanced with increasing dose; at 2.4 mg / mL, the hyaluronidase inhibition rate is as high as 86.86%; however, at the same concentration, the hyaluronic acid inhibition rate of the plant composition of the present invention is slightly lower than that of the positive control ascorbic acid.

[0245] 3) Experimental Example 3: Regulatory Effect on Skin Microorganisms

[0246] In order to verify the regulatory effect of the plant composition of the present invention (containing butterfly pea flower extract) on scalp microorganisms, firstly, Examples 7, 9 and 11 of the present invention were randomly selected to carry out the effect test on the growth of Staphylococcus epidermidis. The test results are detailed in the attached Figure 4 .

[0247] Depend on Figure 4 It can be seen that the mass concentration is 4-16 μg / mL, and the plant composition of the present invention (containing butterfly pea flower extract) shows a significant promoting effect on the growth of Staphylococcus epidermidis, causing Staphylococcus epidermidis to enter the logarithmic growth phase and the stable phase early, and its promoting effect has a significant concentration dependence. When the mass concentration of the plant composition of the present invention (containing butterfly pea flower extract) is 16 μg / mL, the growth promoting effect on Staphylococcus epidermidis is most ideal. Staphylococcus epidermidis has a potential defense function as a skin resident bacterium. It can reduce pathogens on the skin surface, effectively maintain the community structure on the skin surface, and play an important role in skin protection. The experimental results confirm that the plant composition of the present invention (containing butterfly pea flower extract) can effectively promote the growth of scalp probiotics, which is beneficial to regulating the health of the scalp microecology.

[0248] The antibacterial effects of Examples 7-12 of the present invention on Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus were further verified.

[0249] The experimental results are shown in Table 10.

[0250] Table 10 Antibacterial effect of plant compositions (1.0 mg / mL) of Examples 7 to 12 of the present invention

[0251]

[0252] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level. The same below.

[0253] As can be seen from the results of Table 10, the plant compositions of Examples 7 to 12 of the present invention have significant inhibitory effects on Propionibacterium acnes, Malassezia furfur and Staphylococcus aureus. According to the inhibition zone judgment standard, when the concentration is 1.0 mg / mL, the inhibition zone diameter is greater than 15 mm. It can be seen that the plant composition provided by the present invention can effectively inhibit the growth and reproduction of scalp resident bacteria Propionibacterium acnes and Malassezia furfur, as well as transient bacteria Staphylococcus aureus, that is, the plant composition of the present invention (containing butterfly pea flower extract) can effectively regulate the scalp microecological balance.

[0254] 4) Test Example 4: Safety Evaluation

[0255] In order to verify the safety of the plant composition (containing butterfly pea flower extract) of the present invention as a hair care product, a safety evaluation test was conducted on the shampoos prepared in Application Examples 6 to 10 of the present invention.

[0256] Skin and eye irritation test:

[0257] The shampoos prepared in Examples 6 to 10 were subjected to acute skin irritation tests and acute eye irritation tests, respectively. The specific experimental methods, scoring criteria and irritation reaction classifications are all in accordance with the 2015 edition of the Hygienic Standards for Cosmetics.

[0258] The skin irritation reaction results show that: no erythema and no edema occur when using the shampoos prepared by application examples 6 to 10 of the present invention, and the skin irritation level is non-irritating.

[0259] The eye irritation reaction results show that the conjunctiva of the shampoo prepared by application examples 6 to 10 of the present invention has no congestion, no edema, no secretion; the cornea has no turbidity; the iris is normal, and the eye irritation level is non-irritating. The above results show that the shampoo formula of the present invention is mild in nature and non-irritating to the skin and eyes.

[0260] Allergy test (human skin patch test):

[0261] The shampoos prepared in Example 6 to 10 of the present invention were tested with reference to the relevant requirements and determination methods of the "Human Skin Patch Test" in the 2015 edition of the "Cosmetic Hygiene Standards", and the skin reactions were observed at 0h, 6h, 12h, 24h, and 48h, respectively. The experimental results of all volunteer subjects were negative, and no adverse skin reactions occurred. This shows that the shampoo provided by the present invention does not cause allergic reactions, is non-irritating, and has high safety.

[0262] 5) Test Example 5: Evaluation of the use effect

[0263] In order to verify the use effect evaluation of the plant composition (containing butterfly pea flower extract) of the present invention as a hair care product, a user experience evaluation was conducted on the shampoos prepared in Application Examples 6 to 10 of the present invention.

[0264] 100 volunteers with sub-healthy scalp (50 males and 50 females, aged 18 to 50 years old) were selected and randomly divided into 10 groups, with 10 people in each group.

[0265] Usage: Each volunteer uses 20-100 mL of shampoo each time according to the thickness of the hair, massages the head for 5 minutes, and then washes it clean. Shampoo once every other day for two months. Then, the shampoo score is calculated based on the following indicators: soothing, anti-dandruff, anti-itching, oil-water balance, foam abundance, and easy rinsing. The score is based on a 10-point system, with the highest score being 10 and the lowest score being 0. The average score of each volunteer is taken. The results are shown in Table 11.

[0266] Table 11 User experience evaluation

[0267] Group Soothing Relieve itching Anti-dandruff Oil-water balance Foam abundance Easy to rinse Overall rating Application Example 6 9.8 9.7 8.6 9.1 9.7 9.6 9.4 Application Example 7 9.7 9.6 8.5 9.0 9.6 9.5 9.3 Application Example 8 9.8 9.7 8.6 9.1 9.7 9.6 9.4 Application Example 9 9.6 9.5 8.4 8.9 9.5 9.4 9.2 Application Example 10 9.7 9.6 8.5 9.0 9.8 9.5 9.4 .

Claims

1. A plant composition for regulating scalp microecology, characterized in that: The plant composition mainly consists of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Manchurian violet extract / Cephala butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 15-30:15-30:15-30:10-25.

2. The plant composition for regulating scalp microecology according to claim 1, characterized in that: The plant composition is mainly composed of raw materials: Fuding white tea extract, prickly ash extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 18-30:18-30:18-30:15-20.

3. The plant composition for regulating scalp microecology according to claim 1, characterized in that: The plant composition mainly consists of raw materials of Fuding white tea extract, Zanthoxylum bungeanum extract, Northeast Viola extract and oat peptide extract, and the mass ratio of the four is 20-25:20-25:20-25:16-20.

4. The plant composition for regulating scalp microecology according to claim 1, characterized in that: The plant composition is mainly composed of raw materials of Fuding white tea extract, prickly ash extract, butterfly pea flower extract and oat peptide extract, and the mass ratio of the four is 24-28:24-28:24-28:16-18.

5. The plant composition for regulating scalp microecology according to claim 1, characterized in that: The preparation method of the Fuding white tea extract is as follows: placing the Fuding white tea in an extraction tank, then adding ethanol with a volume fraction of 30 to 75% for ultrasonic extraction, the extraction temperature is 40 to 65° C., the extraction time is 60 to 90 minutes, the mass volume ratio of the Fuding white tea and the ethanol is 1 g: 20 to 50 mL, and ultrasonic extraction is performed 2 to 3 times under the same conditions; the obtained extracts are combined, filtered and concentrated to a paste-like crude extract after the combination, and the Fuding white tea extract is obtained; The preparation method of the pepper extract is as follows: crushing pepper into pepper powder, then adding 50-75% ethanol in an amount of 3-30 times the weight of the pepper powder for ultrasonic extraction, the extraction temperature is 40-70° C., the extraction time is 1-8 hours, the obtained extract is filtered and concentrated under reduced pressure in turn, and the pepper extract is obtained; The preparation method of the northeastern violet extract is as follows: placing the northeastern violet in an extraction tank, then adding ethanol with a volume fraction of 70-90% for ultrasonic extraction, the extraction temperature is 45-65° C., the extraction time is 30-60 min, and the mass volume ratio of the northeastern violet to the ethanol is 1 g: 20-40 mL; ultrasonic extraction is performed 3 times under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the northeastern violet extract; The preparation method of the butterfly pea flower extract is as follows: placing butterfly pea flowers in an extraction tank, then adding ethanol with a volume fraction of 55-75% for ultrasonic extraction, the extraction temperature is 40-60° C., the extraction time is 40-60 min, and the mass volume ratio of the butterfly pea flowers and the ethanol is 1 g: 30-50 mL; ultrasonic extraction is performed 2-3 times under the same conditions, the obtained extracts are combined, and after the combination, the extracts are filtered and concentrated to a paste-like crude extract to obtain the butterfly pea flower extract; The preparation method of the oat peptide extract is as follows: weigh oat seeds and grind them, put them into a conical flask after grinding, use acetone to defatted oat flour, discard the supernatant, dry in a fume hood and volatilize the solvent, and collect the oat flour; add 0.1 mol / L Tris-HCl buffer with pH 8.8 to the obtained oat flour according to the mass volume ratio of 1g:12-30mL, and then extract at 30-40°C for 40-90min; then centrifuge at 4°C and 5000r / min for 15min to obtain the supernatant; add an equal volume of 12% trichloroacetic acid to the obtained supernatant to mix, let it stand for 30min after mixing, and centrifuge at 4°C and 10000r / min for 15min to precipitate macromolecular proteins after standing, and the obtained supernatant is the oat peptide extract.

6. Use of the plant composition for regulating scalp microecology according to claim 1 in the preparation of washing and care products.

7. Use of the plant composition according to claim 6 in preparing cleaning products, characterized in that: When preparing the cleaning product, the raw material composition of the cleaning product contains the plant composition for regulating the scalp microecology according to claim 1.

8. Use of the plant composition according to claim 7 in preparing cleaning products, characterized in that: Expressed in terms of mass percentage, the shampoo and conditioner contains 4.5-15% of the plant composition for regulating scalp microecology according to claim 1, 12-24% of a surfactant, 0.5-1.5% of a hair conditioner, 0.5-1.5% of a moisturizer, and water is added to 100%.

9. Use of the plant composition according to claim 8 in preparing cleaning products, characterized in that: The surfactant is at least one of ammonium laureth sulfate, sodium laureth-6 carboxylate, ammonium lauryl sulfate, sodium lauroyl glutamate, sodium cocoyl glutamate and sodium cocoylaminopropionate; The hair conditioning agent is at least one of alanine, proline, threonine, serine, biotin, hydrogenated castor oil, wheat protein and olive oil; The moisturizing agent is at least one of glycerin, allantoin, arginine, betaine and phytosphingosine.

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