Agricultural chemical adhesion synergist based on plant source protein and preparation method and application thereof

Abstract

The invention relates to an agrochemical protein adhesion synergist based on plant-derived protein and a preparation method and application thereof, and belongs to the field of pesticide adjuvants, the agrochemical protein adhesion synergist comprises the following raw materials by weight: 20-500 parts of plant-derived protein, 50-450 parts of a protein dispersant, and 20-350 parts of a protein stabilization protective agent. The product provided by the invention improves the utilization rate of agricultural chemicals, reduces the problems of grain yield reduction, environmental pollution and the like caused by loss of the agricultural chemicals, and can promote plant growth, improve the adversity stress resistance of plants and realize reduction and synergy of the agricultural chemicals along with degradation of proteins into amino acids.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticide adjuvants, and in particular to an agricultural chemical protein adhesion enhancer based on plant protein, and a preparation method and application thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention and should not be regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] Solidly promoting the reduction and efficiency of fertilizers and pesticides is the key to ensuring food security. Agricultural chemicals (such as pesticides, fertilizers, biostimulants, etc.) are important agricultural production materials, and are crucial for preventing diseases and controlling pests and promoting high and stable grain yields. However, due to the super-hydrophobic / hydrophobic properties of the surface of plant leaves and the surface of insect bodies, agricultural chemicals lack adhesion to the surface of plant leaves and the surface of insect bodies, and collisions, bounces or splashes will occur during the spraying process, resulting in more than half of the agricultural chemicals being lost. Even if part of the agricultural chemicals remain on the surface of plant leaves or insect bodies, they will be washed away by rain, and ultimately the agricultural chemicals that work are less than 0.1% of the sprayed amount. The part of agricultural chemicals lost due to rain erosion will enter the soil, pollute groundwater, aggravate environmental pollution, and endanger the ecosystem. The problem of excessive use of agricultural chemicals due to low utilization rate of agricultural chemicals has received widespread attention.

[0004] In order to improve the utilization rate of pesticides and fertilizers, the commonly used method is to add silicone surfactants to pesticides and fertilizers. These surfactants are difficult to degrade, are not friendly to plants and the environment, and may cause secondary environmental pollution.

[0005] Therefore, there is an urgent need for a pesticide adjuvant that is safe, environmentally friendly, low-priced, easily degradable, and resistant to rain erosion. Summary of the invention

[0006] Purpose of the Invention

[0007] The purpose of the present invention is to provide an agricultural chemical protein adhesion enhancer based on plant protein, and a preparation method and application thereof. The agricultural chemical protein adhesion enhancer of the present invention is safe, environmentally friendly, low-priced, easily degradable, resistant to rain erosion, and has significant efficacy of pesticides and fertilizers. It can respond to the national call, promote the reduction and efficacy of pesticides and fertilizers, promote high and stable grain yields, and avoid polluting the environment.

[0008] Solution

[0009] To achieve the purpose of the present invention, in a first aspect, the present invention provides an agricultural chemical protein adhesion enhancer based on plant protein, comprising the following raw materials in parts by weight: 20 to 500 parts of plant protein, 50 to 450 parts of protein dispersant, and 20-350 parts of protein stabilizer.

[0010] Furthermore, the raw materials are included in the following weight ratios: 20-500 parts of plant-based protein, 50-450 parts of protein dispersant, and 20-350 parts of protein stabilizer;

[0011] Optionally, the raw materials are included in the following weight ratios: 200-500 parts of plant-based protein, 50-200 parts of protein dispersant, and 25-100 parts of protein stabilizer;

[0012] Optionally, the raw materials are included in the following weight ratios: 200-500 parts of plant protein, 50-120 parts of protein dispersant, and 25-80 parts of protein stabilizer;

[0013] Optionally, the raw materials are included in the following weight ratios: 300-500 parts of plant-based protein, 50-120 parts of protein dispersant, and 25-50 parts of protein stabilizer.

[0014] Optionally, the plant-derived protein can spontaneously aggregate into a film at an interface.

[0015] Further, the protein dispersant is selected from one or more of polyether sulfonate, chain carboxylate polymer, carboxylate polymer, alkoxy fatty alcohol phosphate, polycarboxylate, sodium lignin sulfonate, phosphate, alkyl naphthalene sulfonate formaldehyde condensation product, naphthalene sulfonate, formaldehyde condensation product, long-chain fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, polyoxyethylene alkylamine, glycerol fatty acid ester, sorbitan fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, sodium alginate, carboxymethyl cellulose, carboxyethyl cellulose, acrylic acid polymer, polyvinyl pyrrolidone, polyacrylamide, anionic surfactant, nonionic surfactant;

[0016] And / or, the protein stabilizer is selected from one or more of ethylene glycol, isopropanol, ethyl ether, propylene oxide, toluene, xylene, methyl acetate, ethyl acetate, propyl acetate, pentane, hexane, octane, cyclohexane, cyclohexanone, toluene cyclohexanone, chlorobenzene, dichlorobenzene, dichloromethane, acetone, methyl butyl ketone, methyl isobutyl ketone, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, acetonitrile, pyridine, phenol, pectin, xanthan gum and other plant / microbial polysaccharides.

[0017] Furthermore, the plant-derived protein is selected from any one or more of the proteins contained in the plants listed in the following table:

[0018]

[0019]

[0020]

[0021]

[0022]

[0023]

[0024]

[0025]

[0026]

[0027]

[0028]

[0029] Further, the plant-based protein is selected from one or more of leguminous plant protein, gramineous plant protein, jugulariaceae plant protein, rosaceae plant protein, malvaceae plant protein, convolvulaceae plant protein, linumaceae plant protein, solanaceae plant protein, compositae plant protein, cruciferous plant protein, bryophyte plant protein, cucurbitaceae plant protein, moraceae plant protein, purslaneaceae plant protein, theaceae plant protein, sesame plant protein, gentianaceae plant protein, sapindaceae plant protein, olive family plant protein, pine family plant protein, nymphaeaceae plant protein, anacardiaceae plant protein, sedge family plant protein, urticaceae plant protein, zingiberaceae plant protein, ginkgoaceae plant protein, cupressaceae plant protein, olive family plant protein, peony family plant protein, ephedraceae plant protein, violaceae plant protein, dioscoreaceae plant protein, brassicaceae plant protein, and osmanthaceae plant protein;

[0030] Optionally, the legume protein includes one or more of soybean protein, peanut protein, mung bean protein, kidney bean protein, black bean protein, pea protein, red bean protein, broad bean protein, and alfalfa protein; Optionally, the Gramineae protein includes one or more of corn protein, oat protein, barley protein, wheat protein, rye protein, buckwheat protein, rice protein, and millet protein; Optionally, the Osmanthus plant protein includes Osmanthus protein; Optionally, the Rosaceae plant protein includes one or more of almond protein and papaya protein; Optionally, the Malvaceae plant protein includes one or more of cottonseed protein and okra protein ; Optionally, the Convolvulaceae plant protein includes one or more of purple potato protein and sweet potato protein; Optionally, the Dioscorea plant protein includes sweet potato protein; Optionally, the Cruciferae plant protein includes one or more of broccoli protein and rapeseed protein; Optionally, the Cucurbitaceae plant protein includes one or more of pumpkin protein, cucumber seed protein, pumpkin seed protein, bitter melon protein, and cucurbit protein; Optionally, the Sapindaceae plant protein includes one or more of litchi protein and longan / longan protein; Optionally, the Nymphaeaceae plant protein includes lotus seed protein; Optionally, the Brasenaceae plant protein includes Brasenia protein; Optionally, The Urticaceae plant protein includes one or more of nettle protein and mulberry leaf protein; optionally, the Juglandaceae includes walnut protein; optionally, the Linaceae plant protein includes flaxseed protein; optionally, the Solanaceae plant protein includes potato protein; optionally, the Asteraceae plant protein includes sunflower seed protein; optionally, the Moss family plant protein includes moss protein; optionally, the Moraceae plant protein includes hemp seed protein; optionally, the Portulacaceae plant protein includes purslane protein; optionally, the Theaceae plant protein includes tea protein; optionally, the Pedunculaceae plant protein includes sesame protein; optionally, the Gentianaceae plant protein includes gentian protein White; Optionally, the Sapotaceae plant protein includes avocado protein; Optionally, the Pinaceae plant protein includes pine nut protein; Optionally, the Anacardiaceae plant protein includes cashew protein; Optionally, the Cyperaceae plant protein includes sedge protein; Optionally, the Zingiberaceae plant protein includes ginger protein; Optionally, the Ephedraceae plant protein includes ephedra protein; Optionally, the Ginkgoaceae plant protein includes ginkgo protein; Optionally, the Cupressaceae plant protein includes cypress berry protein; Optionally, the Oleaceae plant protein includes olive protein; Optionally, the Violaceae plant protein includes violet protein; Optionally, the Paeoniaceae plant protein includes peony protein and rose protein;

[0031] Optionally, water is further included, and the water content is 200 to 800 parts by weight, and optionally 600 to 800 parts by weight.

[0032] Furthermore, one or more of the plant-derived proteins are physically and chemically modified;

[0033] Optionally, the physical modification includes one or more of pH control, salt concentration, heat treatment, ultrasonic treatment, microwave treatment, high pressure treatment, radio frequency treatment, and mechanical stirring;

[0034] Optionally, the chemical modification includes initiating polymerization of monomers containing functional groups from the protein, or grafting functional polymers or small molecules onto the surface of the protein; optionally, the initiating functional groups include one or more of carboxyl, hydroxyl, carbonyl, and amino groups; optionally, the functional polymer used to initiate functional polymerization or grafting includes one or more of polyethylene glycol, polyacrylic acid, polylactic acid, and polysaccharides; optionally, the small molecules used to initiate functional polymerization or grafting include polyphenols, polycarboxylic acids, ketones, glycosides, One or more of plant essential oils, nicotine substances and other plant secondary metabolites; optional polysaccharides include starch, cellulose, algae polysaccharides, chitin / chitin, plant polysaccharides, microbial polysaccharides, anionic polysaccharides, cationic polysaccharides, neutral polysaccharides or more; optional polyphenols include flavonoids such as epicatechin, cyanidin, quercetin, naringenin, etc., phenolic acids such as gallic acid, caffeic acid, chlorogenic acid, rhein, etc., stilbenes such as resveratrol, stilbenes such as jasmonic acid, stilbenes such as echinacea, lignans , tannic acid, etc.; optionally, the polycarboxylic acid includes one or more of oxalic acid, citric acid, malic acid, glycyrrhizic acid, etc.; optionally, the plant essential oil includes one or more of tea tree essential oil, thyme essential oil, and rose essential oil; the chemical modification also includes the introduction of reducing thiol compounds or protein denaturants; optionally, the reducing thiol compounds include one or more of mercaptoethanol, cysteine, glutathione, mercaptosuccinic acid, dihydrolipoic acid, and tris (2-carboxyethyl) phosphine hydrochloride; the protein denaturant includes urea, salt One or more of guanidine acid, guanidine isothiocyanate, sodium dodecyl sulfate, and arginine; chemical modification also includes the use of enzymatic cross-linking or degradation, and optionally the cross-linking enzyme includes one or more of glutamine transaminase, oxidase (tyrosinase, laccase, peroxidase, sulfhydryl oxidase), lipoxygenase and glutamyl endopeptidase; optionally the oxidase includes one or more of tyrosinase, laccase, peroxidase, and sulfhydryl oxidase; optionally the degrading enzyme includes one or more of papain, bromelain and soybean protease.

[0035] In a second aspect, an agricultural chemical preparation is provided, comprising an agricultural chemical and the agricultural chemical protein adhesion enhancer described in the first aspect; optionally, the agricultural chemical comprises one or more of pesticides, fertilizers, and biostimulants.

[0036] Further, the pesticide includes one or more of insecticides, fungicides, antiviral agents, herbicides, miticides, nematicides, rodenticides, plant growth regulators, insect growth regulators, plant immune inducers, fumigants, biological pesticides, mineral source pesticides, organic synthetic pesticides, and seed coating agents;

[0037] and / or, the fertilizer includes one or more of organic fertilizer, chemical fertilizer, compound fertilizer, slow-release fertilizer, biological fertilizer, trace element fertilizer, foliar fertilizer, soil conditioner, and organic-inorganic compound fertilizer;

[0038] And / or, the biostimulants include one or more of humic acid substances, seaweed extracts, free amino acids, complex organic matter, non-organic minerals, microorganisms and their secondary metabolites, plant extracts and their secondary metabolites, chitin and chitosan derivatives, beneficial chemical elements, and anti-transpiration agents;

[0039] Optionally, the target surface of the agricultural chemical is any one of an insect body surface, a plant leaf, a plant root surface, and a seed surface.

[0040] Optionally, the agricultural chemical protein adhesion enhancer is used at a concentration of 5 to 20 mL / 10L, optionally 10 mL / 10L.

[0041] In a third aspect, a method for preparing the agricultural chemical protein adhesion enhancer according to the first aspect or the agricultural chemical preparation according to the second aspect is provided, comprising the following steps:

[0042] 1) First, add the plant-based protein to 200-800 parts of deionized water, and then add the protein dispersant to disperse and mix;

[0043] 2) Adding a protein stabilizer to the protein mixture obtained in step 1), and homogenizing by shearing and sand milling to obtain a stable plant-derived protein adhesive; optionally, obtaining stable plant-derived protein adhesives of different grades of 3nm-60nm.

[0044] It may also include: 3) during implementation, a certain amount of agricultural chemicals is mixed with water or an aqueous solution containing a protein stabilizer, and then the plant-based protein adhesive obtained in step 2) is added, and the product is diluted 200 to 2000 times according to different application scenarios, and the retention amount of agricultural chemicals is increased by spraying, sprinkling, smearing, dipping, spin coating or sputtering on the target surface.

[0045] In a fourth aspect, a use of a plant-based protein in the preparation of an agrochemical protein adhesion enhancer is provided, wherein the plant-based protein is selected from one or more of leguminous plant protein, gramineous plant protein, jugulariaceae plant protein, rosaceae plant protein, Malvaceae plant protein, Convolvulaceae plant protein, linumaceae plant protein, Solanaceae plant protein, Asteraceae plant protein, Cruciferae plant protein, Moss plant protein, Cucurbitaceae plant protein, Moraceae plant protein, Portulacaceae plant protein, Theaceae plant protein, Peduncaceae plant protein, Gentianaceae plant protein, Sapindaceae plant protein, Sapotaceae plant protein, Pinaceae plant protein, Nymphaeaceae plant protein, Anacardiaceae plant protein, Cyperaceae plant protein, Urticaceae plant protein, Zingiberaceae plant protein, Ginkgoaceae plant protein, Cupressaceae plant protein, Oleaceae plant protein, Paeoniaceae plant protein, Ephedraceae plant protein, Violaceae plant protein, Dioscorea plant protein, Brassicaceae plant protein, and Osturtium plant protein.

[0046] The common feature of the proteins contained in the above-mentioned plants is that they can spontaneously aggregate into films at the interface, and are universally and non-antagonistically applicable in agricultural production.

[0047] Beneficial Effects

[0048] The product of the present invention improves the utilization rate of agricultural chemicals, reduces problems such as food production reduction and environmental pollution caused by the loss of agricultural chemicals, and as proteins are degraded into amino acids, it can promote plant growth, improve the plant's ability to resist adverse stress, and achieve a reduction in the amount of agricultural chemicals and an increase in efficiency.

[0049] The protein product of the invention has a simple method of use, and after mixing, the product can be evenly sprayed, smeared, dipped, spin-coated or sputtered onto a target surface.

[0050] The preparation method of the product of the invention is simple, the main component is plant-derived protein, the biocompatibility is high, the product is environmentally friendly, and it is safe for animals and plants.

[0051] In the present invention, the protein product has the characteristics of wide source, low price, environmental friendliness, and resistance to rain erosion, which provides a solid technical foundation for promoting the reduction of chemical fertilizers and pesticides and accelerating the comprehensive green transformation of agriculture. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] One or more embodiments are exemplarily described by the pictures in the accompanying drawings, and these exemplary descriptions do not constitute limitations on the embodiments. The special word "exemplary" here means "used as an example, embodiment or illustrative". Any embodiment described as "exemplary" here is not necessarily interpreted as being superior or better than other embodiments.

[0053] Figure 1: is a scanning electron microscope image of the protein adhesion enhancer product of Example 1 in Test Example 1 of the present invention sprayed on the surface of a blank lotus leaf.

[0054] Figure 2 It is the blank lotus leaf surface in Test Example 2 of the present invention (a), the lotus leaf surface after spraying the pesticide imidacloprid suspension (active ingredient content 70%, solvent is deionized water) (b), and the lotus leaf surface after spraying the protein adhesion enhancer product of Example 2 mixed with the imidacloprid suspension (c).

[0055] Figure 3 This is a fluorescence microscope image of the protein film formed by spraying the mixture of the protein adhesion enhancer product of Example 3 and the imidacloprid suspension in Test Example 3 of the present invention, which shows that the protein film is resistant to rain erosion.

[0056] Figure 4 This is a graph showing the protective effect of the plant-derived protein adhesion enhancer of Example 4 in Test Example 4 of the present invention and the agricultural chemicals (dinotefuran+pyridaben) in a tank mix against whiteflies of pepper.

[0057] Figure 5 This is a graph showing the protective effect of the plant-derived protein adhesion enhancer of Example 5 in Test Example 5 of the present invention and agricultural chemicals (thiamethoxam and spinetoram) in a tank mix against cowpea thrips.

[0058] Figure 6 This is a graph showing the protective effect of the plant-derived protein adhesion enhancer of Example 6 in Test Example 6 of the present invention and agricultural chemicals (chlorfenapyr suspension concentrate, indoxacarb) in a tank mix against rice leaf folder.

[0059] Figure 7 This is a graph showing the protective effect of the plant-derived protein adhesion enhancer of Example 7 in Test Example 7 of the present invention and the agricultural chemical (metalaxyl-M) in a tank mix against grape downy mildew.

[0060] Figure 8 This is a graph showing the improvement in quality and yield of the cruciferous crop Chinese cabbage by tank mixing the plant-based protein adhesion enhancer of Example 8 in Test Example 8 of the present invention with agricultural chemicals (including amino acid foliar fertilizer).

[0061] Fig. 9 This is a graph showing the improvement in quality and yield of Dali winter jujubes by barrel mixing the plant-based protein adhesion enhancer of Example 9 in Test Example 9 of the present invention with agricultural chemicals.

[0062] Fig.10 This is a graph showing the protective effect of the plant-based protein adhesion enhancer of Example 10 in Test Example 10 of the present invention mixed with agricultural chemicals in a barrel against aphid disease of Ruixue apple.

[0063] Fig.11 This is a diagram showing the protective effect of the plant-based protein adhesion enhancer of Example 11 in Test Example 11 of the present invention mixed with agricultural chemicals in a barrel against wheat fusarium wilt disease.

[0064] Fig.12 This is a comparison chart of the synergistic effects of the plant protein enhanced product of Example 2 and animal protein (bovine serum albumin) + reducing agent (TCEP) in Test Example 12 of the present invention. DETAILED DESCRIPTION

[0065] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Unless otherwise expressly stated, throughout the specification and claims, the term "including" or its variations such as "comprising" or "including" will be understood to include the stated elements or components, without excluding other elements or other components.

[0066] In addition, in order to better illustrate the present invention, numerous specific details are provided in the specific embodiments below. It should be understood by those skilled in the art that the present invention can also be implemented without certain specific details. In some embodiments, raw materials, elements, methods, means, etc. well known to those skilled in the art are not described in detail, so as to highlight the main purpose of the present invention.

[0067] The raw materials in the present invention are all commercially available products.

[0068] Example 1

[0069] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 400 parts of soy protein (purchased from Shanghai Yuanye Biotechnology Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0070] Example 2

[0071] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 400 parts of corn protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 80 parts of polyether carboxylate dispersant, 20 parts of phosphate dispersant, 25 parts of ethyl acetate stabilizer and 800 parts of deionized water.

[0072] Example 3

[0073] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding 400 parts of peanut protein (purchased from Xi'an Lingfeng Biotechnology Co., Ltd.), 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer and 800 parts of deionized water.

[0074] Example 4

[0075] The protein adhesion enhancer product of this embodiment is prepared by dispersion, shearing and nano-sand grinding of 400 parts of walnut protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 20 parts of fatty acid polyoxyethylene ester dispersant, 20 parts of sucrose fatty acid ester dispersant, 15 parts of sodium alginate dispersant, 25 parts of pyridine stabilizer, 25 parts of pectin stabilizer and 800 parts of deionized water.

[0076] Example 5

[0077] The protein adhesion enhancer product of the present embodiment is prepared by dispersion, shearing and nano-sand grinding of 100 parts of almond protein (purchased from Shaanxi Xiazhou Biological Co., Ltd.), 100 parts of walnut protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of cottonseed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of purple sweet potato protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 20 parts of long-chain fatty alcohol polyoxyethylene ether dispersant, 20 parts of polyoxyethylene alkylamine dispersant, 15 parts of sodium alginate dispersant, 10 parts of propyl acetate stabilizer, 20 parts of pectin stabilizer, 20 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0078] Example 6

[0079] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 100 parts of oat protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of mung bean protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of potato protein (purchased from Shaanxi Xiazhou Biological Co., Ltd.), 100 parts of flaxseed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 20 parts of sodium lignin sulfonate dispersant, 20 parts of phosphate dispersant, 15 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 20 parts of pectin stabilizer, 20 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0080] Example 7

[0081] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 100 parts of papaya protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of kidney bean protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of sweet potato protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of cottonseed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 20 parts of sodium lignin sulfonate dispersant, 20 parts of phosphate dispersant, 15 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 20 parts of pectin stabilizer, 20 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0082] Example 8

[0083] The protein adhesion enhancer product of this embodiment is prepared by dispersion, shearing and nano-sand grinding of 100 parts of sunflower seed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of black bean protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of sweet potato protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of broccoli protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 20 parts of polyether carboxylate dispersant, 20 parts of phosphate dispersant, 10 parts of naphthalenesulfonate dispersant, 50 parts of ethylene glycol monomethyl ether stabilizer and 800 parts of deionized water.

[0084] Example 9

[0085] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding 400 parts of pea protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 30 parts of fatty acid polyoxyethylene ester dispersant, 30 parts of sucrose fatty acid ester dispersant, 40 parts of sodium alginate dispersant, 25 parts of pectin stabilizer and 800 parts of deionized water.

[0086] Example 10

[0087] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand grinding 400 parts of red bean protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 80 parts of polyether carboxylate dispersant, 20 parts of phosphate dispersant, 25 parts of ethyl acetate stabilizer and 800 parts of deionized water.

[0088] Embodiment 11

[0089] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 400 parts of broad bean protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0090] Example 12

[0091] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding 400 parts of barley protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 30 parts of fatty acid polyoxyethylene ester dispersant, 30 parts of sucrose fatty acid ester dispersant, 40 parts of sodium alginate dispersant, 25 parts of pectin stabilizer and 800 parts of deionized water.

[0092] Example 13

[0093] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand grinding 400 parts of wheat protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer and 800 parts of deionized water.

[0094] Embodiment 14

[0095] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 400 parts of oat protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0096] Embodiment 15

[0097] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-sand milling 400 parts of cottonseed protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0098] Example 16

[0099] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding 100 parts of almond protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of moss protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of rapeseed protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of pumpkin protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 30 parts of sodium lignin sulfonate dispersant, 30 parts of phosphate dispersant, 60 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 10 parts of pectin stabilizer, 5 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0100] Embodiment 17

[0101] The protein adhesion enhancer product of the present embodiment is prepared by dispersing, shearing and nano-sand grinding 100 parts of Osmanthus protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of hemp seed protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of rye protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of cucumber seed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer and 800 parts of deionized water.

[0102] Embodiment 18

[0103] The protein adhesion enhancer product of the present embodiment is prepared by dispersing, shearing and nano-grinding 100 parts of bitter melon protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of pumpkin protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of buckwheat protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of pumpkin seed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 30 parts of sodium lignin sulfonate dispersant, 30 parts of phosphate dispersant, 60 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 10 parts of pectin stabilizer, 5 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0104] Embodiment 19

[0105] The protein adhesion enhancer product of this embodiment is prepared by dispersion, shearing and nano-sand grinding of 100 parts of purslane protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of alfalfa protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of cucurbit protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of tea protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0106] Embodiment 20

[0107] The protein adhesion enhancer product of the present embodiment is prepared by dispersing, shearing and nano-sand milling 100 parts of rice proteins (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of millet proteins (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of gentian proteins (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of sesame proteins (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 40 parts of polyether carboxylate dispersants, 40 parts of phosphate dispersants, 20 parts of naphthalenesulfonate dispersants, 25 parts of ethylene glycol monomethyl ether stabilizers and 800 parts of deionized water.

[0108] Embodiment 21

[0109] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding from 100 parts of lychee protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of avocado protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of pine nut protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of okra protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 30 parts of sodium lignin sulfonate dispersant, 30 parts of phosphate dispersant, 60 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 10 parts of pectin stabilizer, 5 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0110] Embodiment 22

[0111] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding 100 parts of lotus seed protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of cashew nut protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of water shield protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of sedge protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0112] Embodiment 23

[0113] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding from 100 parts of alfalfa protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of longan / longan protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of nettle protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of mulberry leaf protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of polyether carboxylate dispersant, 25 parts of ethylene glycol stabilizer and 800 parts of deionized water.

[0114] Embodiment 24

[0115] The protein adhesion enhancer product of the present embodiment is prepared by dispersing, shearing and nano-sand milling 100 parts of rose protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of ginger protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of ephedra protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of ginkgo protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 30 parts of sodium lignin sulfonate dispersant, 30 parts of phosphate dispersant, 60 parts of sucrose fatty acid ester dispersant, 10 parts of methyl acetate stabilizer, 10 parts of pectin stabilizer, 5 parts of ethylene glycol monoethyl ether stabilizer and 800 parts of deionized water.

[0116] Embodiment 25

[0117] The protein adhesion enhancer product of this embodiment is prepared by dispersing, shearing and nano-grinding from 100 parts of cypress fruit protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 100 parts of olive protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of violet protein (purchased from Xi'an Jinshuo Fruit Industry Co., Ltd.), 100 parts of peony protein (purchased from Shanxi Hengran Biotechnology Co., Ltd.), 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer and 800 parts of deionized water.

[0118] Embodiment 26

[0119] The protein adhesion enhancer product of this embodiment is prepared by physically modifying 400 parts of plant protein (adjusting pH=10, ultrasonication for 30 minutes), adding 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer, and 800 parts of deionized water, and then dispersing, shearing, and nano-sand grinding.

[0120] Embodiment 27

[0121] The protein adhesion enhancer product of this embodiment is prepared by chemically modifying 400 parts of plant protein (grafting functional polymers polyethylene glycol, polylactic acid, polyvinyl alcohol, etc., and this embodiment uses polyvinyl alcohol to modify the white bean alcohol-soluble protein with strong hydrophobicity. References: Zhang Zifan, Wu Sisi, Liu Chunxiu, et al. Research progress on functional properties and modification of kidney bean protein [J]. Food and Fermentation Industries, 2024, 50(05): 357-366. DOI: 10.13995 / j.cnki.11-1802 / ts.034670), and then adding 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer, and 800 parts of deionized water, and preparing the product by dispersion, shearing, and nano-sand milling.

[0122] Embodiment 28

[0123] The protein adhesion enhancer product of this embodiment is prepared by modifying 400 parts of plant protein (blended with polysaccharides, polyphenols, polycarboxylic acids, and plant essential oils; this embodiment uses rice protein modified with glucan, wherein the mass ratio of rice protein to glucan is 1:6, the protein concentration is 4.03 mg / mL, and the reaction time is 22 min. At this time, the maximum grafting degree reaches 48.1%. Reference: Hua Jingxian. Research on the microwave heating graft coupling reaction of rice protein-glucan [D]. Jiangnan University, 2008.), and then adding 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer, and 800 parts of deionized water, and preparing the product by dispersion, shearing, and nano-sand milling.

[0124] Alternatively, a modified material obtained by phosphorylating perilla meal protein and compounding it with chitosan can be used. By means of a free radical grafting method, polyphenol protocatechuic acid (PA) and chitosan can be copolymerized to construct a composite of the two based on hydrogen bonding and electrostatic interactions. References: [1] Zhao Qiaoli. Construction, characterization and performance study of perilla meal protein-based high internal phase emulsion system [D]. Jiangnan University, 2023. DOI: 10.27169 / d.cnki.gwqgu.2023.000050.

[0125] Embodiment 29

[0126] The protein adhesion enhancer product of this embodiment is made of 400 parts of plant protein after chemical modification (adding reducing sulfhydryl compounds cysteine, glutathione, mercaptosuccinic acid, dihydrolipoic acid, etc., and the chemical modification method can be referred to in the literature: An Hongzhou, Chen Jinhuan, Yan Hanxiao, et al. Study on the influence of different modification technologies on the functional properties of plant protein [J / OL]. China Oils and Fats, 1-14 [2024-10-13]. https: / / doi.org / 10.19902 / j.cnki.zgyz.1003-7969.240401. This embodiment uses cysteine-modified rice bran protein, and its preparation method is: under weak alkali (pH=8) and low temperature (45°C), L-cysteine ​​is used as a reducing agent, The amount of reducing agent added was 0.07% (ratio of reducing agent to rice bran protein mass), the action time was 1.5h, the auxiliary ultrasonic treatment was performed, the power was 280W, and the ultrasonic time was 8min. Under this condition, the rice bran protein extraction rate reached 46.59%, which was 16.62%, 9.35%, and 7.4% higher than the alkali method, ultrasonic method, and reducing agent method, respectively, and the rice bran protein purity reached 82%. References: Sun Xiuting. Ultrasonic-reducing agent extraction and modification process of rice bran protein and its functional research [D]. Fujian Agriculture and Forestry University, 2015.), and then 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer, and 800 parts of deionized water were added to obtain the product through dispersion, shearing, and nano-sand milling.

[0127] Embodiment 30

[0128] The protein adhesion enhancer product of this embodiment is prepared by enzymatically decomposing 400 portions of vegetable protein, and adding transglutaminase, tyrosinase, laccase, peroxidase, sulfhydryl oxidase, lipoxygenase, glutamyl endopeptidase, papain, bromelain and soybean protease, etc. (this embodiment uses papain and bromelain to enzymatically hydrolyze soybean protein isolate, and the optimal process for papain to enzymatically hydrolyze SPI is: enzymatic hydrolysis time 3h, enzymatic hydrolysis temperature 60°C, enzyme addition amount 0.35g, pH 7.5, substrate concentration 5g / 100mL; bromelain The optimal process of enzymatic hydrolysis of SPI is: enzymatic hydrolysis time 10h, enzymatic hydrolysis temperature 40℃, enzyme addition amount 0.35g, pH 7.5, substrate concentration 5g / 100mL. References: [1] Chen Yafang. Preparation of soybean oligopeptides and study on their ACE inhibitory activity and chelating properties [D]. Chinese Academy of Agricultural Sciences, 2009.), then add 40 parts of polyether carboxylate dispersant, 40 parts of phosphate dispersant, 20 parts of naphthalenesulfonate dispersant, 25 parts of ethylene glycol monomethyl ether stabilizer, 800 parts of deionized water and prepare it by dispersion, shearing and nano sand milling.

[0129] All parts above are in g, but may also be in kg, mg, etc.

[0130] The present invention can also use other pesticides, fertilizers, plant growth regulators, biostimulants, etc. to replace thiamethoxam, imidacloprid, avermectin, sulfoxaflor, etc. in the above embodiments 1 to 30, use proteins contained in other plants to replace the soybean protein, oat protein, almond protein, sweet potato protein, walnut protein, alfalfa protein, rose protein, etc. in the above embodiments 1 to 30, use other dispersants to replace the phosphates, naphthalenesulfonates, polyether carboxylates, etc. in the above embodiments 1 to 30, and use other stabilizers to replace ethylene glycol, pectin, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, etc. in the above embodiments 1 to 30, all of which are within the protection scope of the present invention.

[0131] In order to prove the advantages of plant protein as a pesticide adjuvant, the inventors conducted performance tests on the protein adhesion enhancer products prepared in Examples 1-30, respectively. The specific tests are as follows:

[0132] Test Example 1. Verification of the film-forming effect of protein adhesion enhancer products

[0133] The target surface was a lotus leaf with super-hydrophobic properties. The diluted protein product of Example 1 was evenly sprayed on the surface of a 5 cm × 5 cm lotus leaf, and allowed to air dry naturally. The film formation was then observed using a scanning electron microscope (see Figure 1 ). The results show that the protein adhesion enhancer product of the present invention can form a film evenly over a large area on the super-hydrophobic lotus leaf surface.

[0134] Test Example 2. Verification of the spreading and adhesion ability of protein adhesion enhancer products

[0135] Test method: Select a protein adhesion enhancer product and 10-100 portions of imidacloprid water dispersible granules (active ingredient content 70%) in a volume ratio of 1:1, dilute 10-2000 times and spray on the lotus leaf surface (super hydrophobic property) as the product group, set only spraying imidacloprid water dispersible granules (without protein adhesion enhancer, the same dilution multiple) as the control group, observe the product spreading and adhesion on the plant leaf surface, and take photos to record. A specific test is:

[0136] Product group: Mix the dilution of imidacloprid (active ingredient content 70%, diluted 10 times with water) and the dilution of the protein adhesion enhancer product of Example 2 in a volume ratio of 1:1, and spray evenly on the surface of 5cm×5cm lotus leaves.

[0137] Control group: only spraying imidacloprid suspension (without adding protein adhesion enhancer, the same dilution multiple).

[0138] Then take photos and record the results. Figure 2 The results showed that the spreading and adhesion area on the lotus leaf surface was small when only the imidacloprid suspension was sprayed (see Figure 2 b), and the protein adhesion enhancer product of the present invention can make the imidacloprid suspension spread and adhere to a large area on the super-hydrophobic lotus leaf surface (see Figure 2 c).

[0139] Test Example 3. Verification of the rainwater resistance of protein adhesion enhancer products

[0140] Test method: Select a protein adhesion enhancer product and mix it with 10-100 parts of imidacloprid water dispersible granules (active ingredient content 70%), dilute it 10-2000 times and spray it on the lotus leaf surface under high pressure as the product group; set up a control group that only sprays imidacloprid water dispersible granules (without protein adhesion enhancer, the same dilution multiple), use tap water to simulate rainwater scouring for 2 minutes, mark it with fluorescent dye, and use a fluorescent microscope to observe the product residue on the lotus leaf surface before and after rainwater scouring, and take photos to record. A specific test is:

[0141] Product group: Mix the dilution of imidacloprid (active ingredient content 70%, diluted 10 times with water) and the dilution of the protein adhesion enhancer product of Example 3 in a volume ratio of 1:1, spray it on the surface of the super-hydrophobic lotus leaf, dry it, and then rinse it with tap water to simulate rain for 2 minutes.

[0142] Control group: The super-hydrophobic surface was sprayed with imidacloprid suspension alone as a control test. After drying, it was washed with tap water to simulate rain for 2 minutes.

[0143] Sodium fluorescein is a fluorescent marker dye, and the results are recorded by taking photos. Figure 3The results showed that the protein product of the present invention had good resistance to rain erosion when observed under a fluorescence microscope.

[0144] Test Example 4. Experiment on the reduction and synergistic control of pepper whitefly by protein adhesion enhancer products

[0145] This test uses the protein adhesion enhancer product of Example 4 and 10-100 parts of 15% cypermethrin (emulsifiable concentrate) and 20% dimethoate (suspension concentrate) diluted 500-2000 times respectively and then mixed. A test on the effect of reducing dosage and enhancing synergy in the process of chemical control of pepper whitefly was carried out in Dongsun Village, Yanliang District, Xi'an City, Shaanxi Province. The test had 3 treatment groups and 1 control group. Each treatment used 10L of water and the pesticide was applied once by foliar spray (the specific test treatment method is shown in Table 1). The death of pests was checked on the 3rd day after the drug was applied, and the mortality rate was counted 7 days after the drug was applied. The results of Example 4 are shown in Table 1. Figure 4 .

[0146] Table 1 Experimental treatment

[0147]

[0148] Table 1 and Figure 4 In the figure, 100% represents the initial use of pesticides, and 75% represents the reduction of the relative initial use of pesticides to 75%.

[0149] Figure 4 The results showed that the protein synergist product of Example 4 was mixed with the highly permeable insecticide dinotefuran, and no pesticide damage occurred to peppers. After spraying for 7 days, the number of whiteflies killed in the 100% pesticide group and the "100% + synergist" group was compared, and it was found that the use of the protein synergist product of the present invention can increase the adhesion of pesticides on vegetable leaves, promote better efficacy, and thus increase the number of whiteflies killed. Comparing the number of whiteflies killed in the 100% pesticide group and the "75% + synergist" group, it was found that the use of the protein synergist product of the present invention can reduce the use of the pesticide pyridaben by 25%.

[0150] It has been verified that the protein enhancer products of Examples 5 to 7 can also reduce the usage of the pesticide pyridabenzan by at least 25%.

[0151] Therefore, the protein synergist product of the present invention will not cause pesticide damage when used in a tank mix with the highly permeable neonicotinoid insecticide dinotefuran, and has an obvious effect of reducing dosage and increasing synergy, which can reduce the usage of the pesticide pyridaben by 25%.

[0152] Test Example 5. Experiment on the reduction and synergistic control of cowpea thrips by using protein adhesion enhancer products

[0153] The test used the protein adhesion enhancer product of Example 8, and the location was set up at the Guilinyang Agricultural Vegetable Base in Meilan District, Haikou City. The test area was about 1.5 acres, the soil type was sandy, the pH value was 5.5, and the organic matter content was 11.1 g / kg. The test was applied once during the flowering period of cowpea, and the water used for application was 60 L / acre (the specific test treatment method is shown in Table 2). The control effect was statistically analyzed 3 days and 6 days after the application. The field control effect results of the protein adhesion enhancer product of Example 8 after mixing with the agent are shown in Figure 5 .

[0154] Table 2 Experimental treatment

[0155]

[0156] Figure 5 The results showed that the addition of the protein synergist of Example 8 to the pesticide had a synergistic effect on the control of cowpea thrips, that is, the amount of pesticide used could be reduced by 70%. The synergistic effect was most obvious 3 days after the application of the pesticide.

[0157] It has been verified that the protein enhancer products of Examples 9 to 11 can also reduce the pesticide usage of thiamethoxam or spinetoram by at least 30%.

[0158] The protein synergist product of the present invention will not cause pesticide damage when mixed with the insecticides thiamethoxam and spinetoram in a barrel, and has an obvious effect of reducing the dosage and increasing the synergy, and can reduce the amount of pesticide used by 25%-30%.

[0159] Test Example 6. Experiment on the reduction and synergistic effect of protein adhesion enhancer products on rice leaf roller

[0160] The test used the protein adhesion enhancer product of Example 12, and the location was set up at the Zhaofeng planting base in Tanxia Town, Lingchuan County, Guangxi, and the rice variety was "Deyou 108". The test was applied once at the rice heading stage, and the water used for application was 30L / mu (the specific test treatment method is shown in Table 3). After the protein adhesion enhancer product of Example 12 was mixed with the drug, the control effect was statistically analyzed 3 days, 7 days and 14 days after the drug was applied (see Figure 6 ).

[0161] Table 3 Experimental treatment

[0162]

[0163] Figure 6 The results showed that compared with the control effect of a single dose of pesticide, the addition of the protein synergist of Example 12 to the pesticide had a significant synergistic effect on the control of rice leaf rollers; after the pesticide dosage was reduced by 30% and the protein synergist was added, the control effect was better than that of the pesticide group at a single dosage of 100%. The control effect was most obvious 7 days after the application of the pesticide.

[0164] It has been verified that the protein enhancer products of Examples 13 to 15 can also reduce the pesticide usage of chlorantraniliprole or indoxacarb by at least 30%.

[0165] The protein synergist product of the present invention will not cause pesticide damage when used in a barrel mix with the insecticides chlorfenapyr and indoxacarb, and has an obvious effect of reducing the dosage and increasing the synergy, thereby reducing the amount of pesticide used by 30%.

[0166] Test Example 7. Experiment on the reduction and synergistic effect of protein adhesion enhancer products on the control of grape downy mildew

[0167] The test was conducted using the protein adhesion enhancer product of Example 16 at the Xi'an Urban Agriculture Experimental Demonstration Station in Chang'an District, Xi'an. The variety was Hutai No. 8, which was 8 years old and had a cultivation size of 2.4m×1.0m. When the pesticide was applied, the grapes were in the fruit expansion stage, the early stage of downy mildew. The pesticide was applied three times in total, with 15L / mu of water for application (the specific test treatment method is shown in Table 4). The control effect was counted after each application. The control effect of the protein adhesion enhancer product of Example 16 after mixing with the agent was shown in Table 4. Figure 7 .

[0168] Table 4 Experimental treatments

[0169] deal with Pharmacy 1 15% oxadiazine·metalaxyl 10mL / 10L water 2 15% oxadiazine·metalaxyl 10mL / 10L water + protein enhancer 10mL / 10L water 3 15% oxadiazine·metalaxyl 8mL / 10L water + protein enhancer 10mL / 10L water 4 Blank control (protein enhancer 10mL / 10L water)

[0170] Figure 7 The results showed that compared with the control effect of a single pesticide, the addition of protein enhancers to pesticides had a significant synergistic effect on the prevention and control of grape downy mildew; after the pesticide dosage was reduced by 20% and protein enhancers were added, the control effect was better than the pesticide group when the single dosage was 100%.

[0171] It has been verified that the protein enhancer products of Examples 17 to 19 can also reduce the pesticide usage of oxadiazine-M and metalaxyl-M by at least 20%.

[0172] The protein synergist product of the present invention will not cause pesticide damage when mixed with oxadiazine and metalaxyl-M in a tank, and has an obvious effect of reducing the dosage and increasing the synergy, and can reduce the amount of pesticide used by 20%.

[0173] Test Example 8. Experiment on reducing the amount of green cabbage, increasing its efficiency, improving its quality and increasing its yield by using protein adhesion enhancer

[0174] The protein adhesion synergist product of Example 20 was used in the test. The location was Kangli Plantation, Weizhao 3 Village, Wangmang Street, Chang'an District, Xi'an City, Shaanxi Province, to carry out the synergistic test of the Chinese cabbage heart protein synergist. The test set up 2 groups, each group used 10L / mu of water for fertilization, and fertilized once every 15 days, for a total of 3 times (the specific test treatment method is shown in Table 5). After harvest, the growth indicators and quality of each treated Chinese cabbage heart were tested, including stem diameter, stem length, fresh weight, dry weight, water content, chlorophyll content, vitamin content, protein content, and per mu yield. The effects of the protein adhesion synergist product of Example 20 mixed with the amino acid foliar fertilizer are shown in Tables 6, 7, Figure 8 .

[0175] Table 5 Experimental treatment

[0176]

[0177] Table 6 Growth indexes of Chinese cabbage during the application of protein enhancer in Chinese cabbage foliar fertilizer

[0178]

[0179] Table 7 The results of the determination of nutritional indexes of Chinese cabbage during the application of protein enhancer in Chinese cabbage foliar fertilizer

[0180]

[0181] Figure 8 The test results showed that the chlorophyll content, vitamin C content and protein content of the cauliflower in the enhanced group of Example 20 were significantly higher than those in the control group, with fewer diseases and insect pests, richer leaf color, a 5% increase in yield, and some cauliflower in the enhanced group bloomed early, proving that the synergist had a certain effect of promoting early maturity.

[0182] It has been verified that the protein enhancer products of Examples 21 to 23 can also improve the quality and yield of Chinese cabbage core.

[0183] Therefore, when using foliar fertilizer for Chinese cabbage core, the protein enhancer product of the present invention can be reasonably added and used to improve the quality and yield of Chinese cabbage core.

[0184] Test Example 9. Experiment on the effect of protein adhesion enhancer on reducing the amount of Dali winter jujube

[0185] The test used the protein adhesion enhancer product of Example 24 to carry out a Dali winter jujube pesticide reduction and efficiency test at the Fruit Industry Development Center of Dali County, Weinan City, Shaanxi Province. The test set up 5 treatments (specific test treatment methods are shown in Table 8), each treatment used 60L of water, and applied the pesticide 5 times in total. After the fruits were harvested in September, the yield and quality of each treatment were tested, including single fruit weight, fruit shape index, pulp hardness, fruit soluble solids content, titratable acid content, and pesticide residues. The effects of the protein adhesion enhancer product of Example 24 after mixing with pesticides are shown in Tables 9, 10, Fig. 9 .

[0186] Table 8 Test treatment

[0187]

[0188] In Table 8, the synergist refers to the protein adhesion synergist of Example 24, and its added amount is 10mL / 10L water (i.e., the protein adhesion synergist of Examples 24-26 is diluted 1000 times), and 90%, 70%, 50%, and 30% mean that the amount of pesticide added is 90%, 70%, 50%, and 30% of the recommended amount.

[0189] Table 9 Fruit shape index of Dali winter jujube

[0190]

[0191] Table 10 Pesticide residues in Dali winter jujube

[0192]

[0193] The results showed that the average single fruit weight of the five treatment groups (using the protein adhesion enhancer product of Example 24) all met the special fruit standard of single fruit weight of more than 18g as stipulated in the Shaanxi Provincial Local Standard DB61 "Geographical Indication Product Dali Winter Date" issued in 2020 (see Fig. 9 The single fruit weight of the 50% and 70% reduction groups was significantly higher than that of the control group (CK), increasing by 16.83% and 49.67% respectively (see Fig. 9 ). The soluble solids content of the jujube fruit in the 50% reduction group was significantly higher than that in the control group. The fruit shape index of the control group, 30% reduction group, and 50% reduction group was greater than 1, and was oblong. The fruit shape index of the 70% reduction group and the 90% reduction group tended to 1, and was round (see Table 9). The pesticide residue in the 50% reduction group was significantly lower than that in the control group, and the pesticide residue in the reduction group was lower than the maximum allowable residue (see Table 10). It has been verified that the protein enhancer products of Examples 25 and 26 can also increase the weight of a single fruit and reduce the pesticide residue when the dosage is reduced by 30%, 50%, and 70%.

[0194] In summary, the protein enhancer product of the present invention can be reasonably added and used to improve the quality of winter jujube.

[0195] Test Example 10. Experiment on reducing the amount and increasing the efficacy of protein adhesion enhancer products in controlling apple aphid diseases

[0196] The test used the protein adhesion enhancer product of Example 27 to carry out a pesticide reduction and synergistic control test of Ruixue apple aphid disease in Caojia Village, Leiya Town, Baishui County, Weinan City, Shaanxi Province. The test set up 5 treatments (specific test treatment methods are shown in Table 11), each treatment used 30L of water, and the pesticide was applied once. The aphid count was investigated once 1, 3, and 7 days after the drug was applied, and the control effect was calculated. The control effect of the protein adhesion enhancer product of Example 27 after mixing with the pesticide was shown in Table 11. Fig.10 .

[0197] Table 11 Experimental treatment

[0198] In Table 11, the amount of protein enhancer added is 10 mL of the protein adhesion enhancer product of Example 10 per 10 L of water, that is, the final concentration of the protein adhesion enhancer of Example 27 in the spray is 10 mL / 10 L.

[0199]

[0200] The experimental results show that the chemical insecticide has a significant synergistic effect on the control of apple aphids after being mixed with the protein synergist of Example 27. The control effect of flonicamid·bifenthrin mixed with the protein synergist is significantly better than that of the conventional efficacy group at 1, 3, and 7 days, and the difference in the control effect at 1 and 3 days is significant. The control effect of flonicamid·bifenthrin mixed with the protein synergist after a 30% reduction is significantly better than that of the conventional efficacy group without reduction, and it is better than the conventional synergistic adjuvant Anrongle 7 days after the drug is used.

[0201] It has been verified that the protein enhancer products of Examples 28 to 30 can reduce the amount of pesticides by at least 30%.

[0202] Therefore, the protein synergist product of the present invention can be reasonably added and used in combination with the insecticide flonicamid·bifenthrin to help prevent and control apple aphids.

[0203] Test Example 11. Experiment on the reduction and synergistic effect of protein adhesion enhancer products on wheat fusarium disease

[0204] The test used the protein adhesion enhancer product of Example 11 to carry out a pesticide reduction and synergistic prevention test of wheat fusarium disease in the wheat field of Kangmeiyuan Ecological Agriculture Cooperative in Shangguodian Village, Fengxiang District, Baoji City, Shaanxi Province. The test set up 9 treatments (Table 12), with a water consumption of 30L for each treatment, and a total of 2 applications. The initial disease situation was investigated before application. Since no disease occurred during application, the base number before application was zero. Seven days after the second application, the fusarium disease index was investigated to calculate the prevention effect. At the same time, the safety after application was recorded, and the number of ears per mu was investigated after harvest. Each treatment area is 0.3 mu, repeated 3 times. The clear water control area is 0.1 mu, repeated 3 times. The treatment areas are randomly arranged, and the field test areas are arranged as follows:

[0205]

[0206] Sampling was conducted at 5 points in each plot, with 100 ears surveyed at each point, with 3 replicates. The proportion of diseased spikelets showing ear rot symptoms (or white ear symptoms caused by stem rot) to all spikelets was divided into 5 levels.

[0207] Level 0: The whole ear is disease-free;

[0208] Level 1: The number of diseased spikelets accounts for less than 1 / 4 of all spikelets;

[0209] Level 2: The number of diseased spikelets accounts for 1 / 4 to 1 / 2 of all spikelets;

[0210] Level 3: The number of diseased spikelets accounts for 1 / 2 to 3 / 4 of all spikelets;

[0211] Level 4: The number of diseased spikelets accounts for more than 3 / 4 of all spikelets.

[0212]

[0213] The base number of disease conditions before medication was investigated, and the prevention effect was calculated using the following formula:

[0214]

[0215] Table 12 Experimental treatment

[0216] deal with Experimental Drugs Dilution multiple 1 Blank control -- 2 45% Tebuconazole·Prochloraz Emulsion in Water Recommended dose 3 Protein enhancer + 40% tebuconazole·prochloraz emulsion in water Enhancer diluted 1000 times + recommended dose 4 Protein enhancer + 40% tebuconazole·prochloraz emulsion in water Synergist dilution 1000 times + pesticide reduction 30% 5 50% methyl thiophanate suspension Recommended dose 6 Protein enhancer + 50% methyl thiophanate suspension Enhancer diluted 1000 times + recommended dose 7 Protein enhancer + 50% methyl thiophanate suspension Synergist dilution 1000 times + pesticide reduction 30% 8 Anrongle + 40% tebuconazole·prochloraz emulsion in water Recommended dose + Anrongle 9 Anrongle + 50% methyl thiophanate suspension Recommended dose + Anrongle

[0217] Note: Anrongle is a lecithin vitamin E suspension emulsion, which is commercially available.

[0218] In Table 11, the synergist was diluted 1000 times, which means that the final concentration of the protein adhesion enhancer of Example 11 in the spray was 10 mL / 10 L.

[0219] The test results showed that the wheat in the treatment area did not lose green color, deformity, or die seedlings, which were not conducive to wheat growth, when observed 1, 3, 5, and 7 days after the application of the pesticide. No abnormal death was observed when beneficial insects, such as ladybugs and aphid flies, were released into the wheat field, and their growth and feeding were normal, indicating that the protein enhancer diluted 1000 times and mixed with 45% tebuconazole·prochloraz emulsion and 50% methyl thiophanate suspension for spraying was safe for wheat and had no effect on other organisms in the field.

[0220] Seven days after the second application, the overall incidence of wheat fusarium head blight was relatively mild, with a low diseased ear rate. The incidence of the blank control was 17.29%, the incidence of the four treatment groups of tebuconazole and prochloraz was 10-11%, and the incidence of the four treatment groups of methyl thiophanate was 8-9%. Fig.11 ), the control effect of pentothazole·prochloraz+Anrongle treatment group and single-dose pentothazole·prochloraz on wheat scab was equivalent, and the control effect of pentothazole·prochloraz+protein synergist and pentothazole·prochloraz+protein synergist with a reduction of 30% on wheat scab were significantly better than single-dose pentothazole·prochloraz, indicating that the synergistic effect of the protein synergist product of the present invention after mixing with pesticides in a tank is better than the commercially available synergist Anrongle. The control effect of methyl thiophanate-methyl + protein synergist and methyl thiophanate-methyl + protein synergist with a reduction of 30% on wheat scab were better than the control effect of single-dose methyl thiophanate-methyl on wheat scab. The above results show that the reasonable addition and use of the protein synergist product of the present invention can significantly improve the control effect of wheat scab.

[0221] The present invention can use other pesticides, fertilizers or biostimulants to replace the pesticides, fertilizers and biostimulants such as imidacloprid, dinotefuran, pyridaben, thiamethoxam, spinetoram, chlorantraniliprole, indoxacarb, metalaxyl-M, and amino acid-containing foliar fertilizers in the above-mentioned embodiments 1 to 30. Other plant-based proteins can be used to replace the proteins used in the above-mentioned embodiments 1 to 30, all of which are within the scope of protection of the present invention.

[0222] Comparative Example 1

[0223] Using animal protein as film-forming agent: Comparison of animal protein (bovine serum albumin, 10 mg / ml) + reducing agent (TCEP, 50 mM), the results are as follows Fig.12 The results show that the synergistic effect of animal protein + reducing agent on the pesticide imidacloprid is lower than that of single plant protein (protein adhesion enhancer in Example 2).

[0224] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An agricultural chemical protein adhesion enhancer based on plant-derived protein, characterized in that: The invention comprises the following raw materials in proportion by weight: 20 to 500 parts of plant source protein, 50 to 450 parts of protein dispersant and 20 to 350 parts of protein stabilizing protective agent.

2. The agricultural chemical protein adhesion enhancer according to claim 1, characterized in that: The invention comprises the following raw materials in the following weight proportions: 20 to 500 parts of plant-based protein, 50 to 450 parts of a protein dispersant, and 20 to 350 parts of a protein stabilizer; Optionally, the raw materials are included in the following weight ratios: 200-500 parts of plant-based protein, 50-200 parts of protein dispersant, and 25-100 parts of protein stabilizer; Optionally, the raw materials are included in the following weight ratios: 200-500 parts of plant-based protein, 50-120 parts of protein dispersant, and 25-80 parts of protein stabilizer; Optionally, the raw materials are included in the following weight ratios: 300-500 parts of plant protein, 50-120 parts of protein dispersant, and 25-50 parts of protein stabilizer; Optionally, the plant-derived protein can spontaneously aggregate into a film at an interface.

3. The agricultural chemical protein adhesion enhancer according to claim 1 or 2, characterized in that: The protein dispersant is selected from one or more of polyether sulfonate, chain carboxylate polymer, carboxylate polymer, alkoxy fatty alcohol phosphate, polycarboxylate, sodium lignin sulfonate, phosphate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, formaldehyde condensate, long-chain fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, polyoxyethylene alkylamine, glycerol fatty acid ester, sorbitan fatty acid ester, sorbitan fatty acid ester, sucrose fatty acid ester, sodium alginate, carboxymethyl cellulose, carboxyethyl cellulose, acrylic acid polymer, polyvinyl pyrrolidone, polyacrylamide, anionic surfactant, nonionic surfactant; And / or, the protein stabilizer is selected from one or more of ethylene glycol, isopropanol, ethyl ether, propylene oxide, toluene, xylene, methyl acetate, ethyl acetate, propyl acetate, pentane, hexane, octane, cyclohexane, cyclohexanone, toluene cyclohexanone, chlorobenzene, dichlorobenzene, dichloromethane, acetone, methyl butyl ketone, methyl isobutyl ketone, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, acetonitrile, pyridine, phenol, pectin, xanthan gum and other plant / microbial polysaccharides.

4. The agricultural chemical protein adhesion enhancer according to any one of claims 1 to 3, characterized in that: The plant-derived protein is selected from any one or more of the proteins contained in the plants listed in the following table:

5. The agricultural chemical protein adhesion enhancer according to any one of claims 1 to 4, characterized in that: The plant-based protein is selected from one or more of leguminous plant protein, gramineous plant protein, jugulariaceae plant protein, rosaceae plant protein, malvaceae plant protein, convolvulaceae plant protein, linumaceae plant protein, solanaceae plant protein, compositae plant protein, cruciferous plant protein, bryophyte plant protein, cucurbitaceae plant protein, moraceae plant protein, purslane plant protein, theaceae plant protein, sesame plant protein, gentianaceae plant protein, sapindaceae plant protein, olive family plant protein, pine family plant protein, nymphaeaceae plant protein, anacardiaceae plant protein, sedge family plant protein, urticaceae plant protein, zingiberaceae plant protein, ginkgoaceae plant protein, cupressaceae plant protein, olive family plant protein, peony family plant protein, ephedraceae plant protein, violaceae plant protein, Dioscorea family plant protein, Brassicaceae plant protein, and Ostramonium family plant protein; Optionally, the legume protein includes one or more of soybean protein, peanut protein, mung bean protein, kidney bean protein, black bean protein, pea protein, red bean protein, broad bean protein, and alfalfa protein; Optionally, the Gramineae protein includes one or more of corn protein, oat protein, barley protein, wheat protein, rye protein, buckwheat protein, rice protein, and millet protein; Optionally, the Osmanthus plant protein includes Osmanthus protein; Optionally, the Rosaceae plant protein includes one or more of almond protein and papaya protein; Optionally, the Malvaceae plant protein includes one or more of cottonseed protein and okra protein ; Optionally, the Convolvulaceae plant protein includes one or more of purple potato protein and sweet potato protein; Optionally, the Dioscorea plant protein includes sweet potato protein; Optionally, the Cruciferae plant protein includes one or more of broccoli protein and rapeseed protein; Optionally, the Cucurbitaceae plant protein includes one or more of pumpkin protein, cucumber seed protein, pumpkin seed protein, bitter melon protein, and cucurbit protein; Optionally, the Sapindaceae plant protein includes one or more of litchi protein and longan / longan protein; Optionally, the Nymphaeaceae plant protein includes lotus seed protein; Optionally, the Brasenaceae plant protein includes Brasenia protein; Optionally, The Urticaceae plant protein includes one or more of nettle protein and mulberry leaf protein; optionally, the Juglandaceae includes walnut protein; optionally, the Linaceae plant protein includes flaxseed protein; optionally, the Solanaceae plant protein includes potato protein; optionally, the Asteraceae plant protein includes sunflower seed protein; optionally, the Moss family plant protein includes moss protein; optionally, the Moraceae plant protein includes hemp seed protein; optionally, the Portulacaceae plant protein includes purslane protein; optionally, the Theaceae plant protein includes tea protein; optionally, the Pedunculaceae plant protein includes sesame protein; optionally, the Gentianaceae plant protein includes gentian protein White; Optionally, the Sapotaceae plant protein includes avocado protein; Optionally, the Pinaceae plant protein includes pine nut protein; Optionally, the Anacardiaceae plant protein includes cashew protein; Optionally, the Cyperaceae plant protein includes sedge protein; Optionally, the Zingiberaceae plant protein includes ginger protein; Optionally, the Ephedraceae plant protein includes ephedra protein; Optionally, the Ginkgoaceae plant protein includes ginkgo protein; Optionally, the Cupressaceae plant protein includes cypress berry protein; Optionally, the Oleaceae plant protein includes olive protein; Optionally, the Violaceae plant protein includes violet protein; Optionally, the Paeoniaceae plant protein includes peony protein and rose protein; Optionally, water is further included, and the water content is 200 to 800 parts by weight, and optionally 600 to 800 parts by weight.

6. The agricultural chemical protein adhesion enhancer according to any one of claims 1 to 5, characterized in that: One or more of the plant-derived proteins are physically and chemically modified; Optionally, the physical modification includes one or more of pH control, salt concentration, heat treatment, ultrasonic treatment, microwave treatment, high pressure treatment, radio frequency treatment, and mechanical stirring; Optionally, the chemical modification includes initiating polymerization of monomers containing functional groups from the protein, or grafting functional polymers or small molecules onto the surface of the protein; optionally, the initiating functional groups include one or more of carboxyl, hydroxyl, carbonyl, and amino groups; optionally, the functional polymer used to initiate functional polymerization or grafting includes one or more of polyethylene glycol, polyacrylic acid, polylactic acid, and polysaccharides; optionally, the small molecules used to initiate functional polymerization or grafting include polyphenols, polycarboxylic acids, ketones, glycosides, One or more of plant essential oils, nicotine substances and other plant secondary metabolites; optional polysaccharides include starch, cellulose, algae polysaccharides, chitin / chitin, plant polysaccharides, microbial polysaccharides, anionic polysaccharides, cationic polysaccharides, neutral polysaccharides or more; optional polyphenols include flavonoids such as epicatechin, cyanidin, quercetin, naringenin, etc., phenolic acids such as gallic acid, caffeic acid, chlorogenic acid, rhein, etc., stilbenes such as resveratrol, stilbenes such as jasmonic acid, stilbenes such as echinacea, lignans , tannic acid, etc.; optionally, the polycarboxylic acid includes one or more of oxalic acid, citric acid, malic acid, glycyrrhizic acid, etc.; optionally, the plant essential oil includes one or more of tea tree essential oil, thyme essential oil, and rose essential oil; the chemical modification also includes the introduction of reducing thiol compounds or protein denaturants; optionally, the reducing thiol compounds include one or more of mercaptoethanol, cysteine, glutathione, mercaptosuccinic acid, dihydrolipoic acid, and tris (2-carboxyethyl) phosphine hydrochloride; the protein denaturant includes urea, salt One or more of guanidine acid, guanidine isothiocyanate, sodium dodecyl sulfate, and arginine; chemical modification also includes the use of enzymatic cross-linking or degradation, and optionally the cross-linking enzyme includes one or more of glutamine transaminase, oxidase (tyrosinase, laccase, peroxidase, sulfhydryl oxidase), lipoxygenase and glutamyl endopeptidase; optionally the oxidase includes one or more of tyrosinase, laccase, peroxidase, and sulfhydryl oxidase; optionally the degrading enzyme includes one or more of papain, bromelain and soybean protease.

7. An agricultural chemical preparation, characterized in that: It comprises agricultural chemicals and the agricultural chemical protein adhesion enhancer according to any one of claims 1 to 6; optionally, the agricultural chemicals comprise one or more of pesticides, fertilizers and biostimulants.

8. The agricultural chemical formulation according to claim 7, characterized in that Pesticides include one or more of insecticides, fungicides, antiviral agents, herbicides, miticides, nematicides, rodenticides, plant growth regulators, insect growth regulators, plant immune inducers, fumigants, biological pesticides, mineral-derived pesticides, organic synthetic pesticides, and seed coating agents; and / or, the fertilizer includes one or more of organic fertilizer, chemical fertilizer, compound fertilizer, slow-release fertilizer, biological fertilizer, trace element fertilizer, foliar fertilizer, soil conditioner, and organic-inorganic compound fertilizer; And / or, the biostimulants include one or more of humic acid substances, seaweed extracts, free amino acids, complex organic matter, non-organic minerals, microorganisms and their secondary metabolites, plant extracts and their secondary metabolites, chitin and chitosan derivatives, beneficial chemical elements, and anti-transpiration agents; Optionally, the target surface of the agricultural chemical is any one of the surface of insect bodies, plant leaves, plant root surfaces, and seed surfaces; Optionally, the agricultural chemical protein adhesion enhancer is used at a concentration of 5 to 20 mL / 10L, optionally 10 mL / 10L.

9. A method for preparing the agricultural chemical protein adhesion enhancer according to any one of claims 1 to 6, or the agricultural chemical preparation according to any one of claim 7, comprising the following steps: 1) First, add the plant-based protein to 200-800 parts of deionized water, and then add the protein dispersant to disperse and mix; 2) Adding a protein stabilizer to the protein mixture obtained in step 1), and homogenizing by shearing and sand milling to obtain a stable plant-derived protein adhesive; optionally, obtaining stable plant-derived protein adhesives of different grades of 3nm-60nm.

10. Use of plant-derived protein in the preparation of an agricultural chemical protein adhesion enhancer, characterized in that: The plant-based protein is selected from one or more of leguminous plant protein, gramineous plant protein, jugulariaceae plant protein, rosaceae plant protein, malvaceae plant protein, convolvulaceae plant protein, linumaceae plant protein, solanaceae plant protein, compositae plant protein, cruciferous plant protein, bryophyte plant protein, cucurbitaceae plant protein, moraceae plant protein, purslaneaceae plant protein, theaceae plant protein, sesame plant protein, gentianaceae plant protein, sapindaceae plant protein, olive family plant protein, pine family plant protein, nymphaeaceae plant protein, anacardiaceae plant protein, sedge family protein, urticaceae plant protein, zingiberaceae plant protein, ginkgoaceae plant protein, cupressaceae plant protein, olive family plant protein, peony family plant protein, ephedraceae plant protein, violaceae plant protein, Dioscorea family plant protein, Brassicaceae plant protein, and Ostracinia family plant protein.

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