Feed additive and application

Abstract

The invention provides a feed additive and application, and belongs to the technical field of feed additives. The invention provides a feed additive which can be applied to preparation of feed for promoting weight gain of mutton sheep and preventing enteritis. The feed additive is a compound traditional Chinese medicine preparation, the components of the feed additive are clear, easy to obtain and simple to prepare, all the components are definite effective components extracted from Chinese herbal medicines, and the feed additive has good prevention and treatment effects on inflammatory diarrhea easily generated in the fattening stage of weaned lambs. The problems of drug resistance and drug residues caused by western medicines in the prior art are solved, and the problems of unclear effective components and unknown content of compound traditional Chinese medicines in the prior art are also solved.

Description

Technical Field

[0001] The invention belongs to the technical field of feed additives, and particularly relates to the application of a compound Chinese medicine combination in preparing feed for promoting weight gain of mutton sheep and preventing enteritis. Background Art

[0002] Mutton sheep are one of the most adaptable livestock to the external environment. They have a wide range of diets, are tolerant to roughage, and have strong resistance to adversity. Raising mutton sheep requires little investment, has a fast turnover, stable benefits, and a high rate of return. Mutton sheep are large, grow fast, have more muscles, and less fat: they have well-developed leg muscles, a cylindrical body, and less fat. They grow fast in the early stages and are famous for their high meat production performance and more lean meat in the carcass. The protein content of mutton sheep is as high as 8%-9.5%, and the digestibility after human consumption is as high as over 90%. Mutton can provide a large amount of heat energy, which is more than twice that of pork. Therefore, mutton has long been favored by consumers. The grade of mutton sheep is significantly higher than that of ordinary sheep. The meat is bright red, the texture is fine and elastic, the marble pattern is moderate, the fat color is white or light yellow, and the fat coverage rate of the carcass surface is 100%. It is difficult for ordinary mutton to meet this standard. Therefore, it is very important to develop a method for increasing the weight of mutton sheep without residue and toxic side effects.

[0003] But in the breeding process. Gastroenteritis is one of the main diseases of diarrhea in large-scale sheep farms. Sheep gastroenteritis refers to severe inflammation of the surface and deep tissues of the gastrointestinal wall. It is mainly caused by improper feeding and management, poor feed quality, overeating, sudden feed changes, eating poisonous plants, damp pens, etc. It manifests as depression, lack of appetite, thick tongue coating, bad breath, porridge-like or watery feces, fishy smell, mixed with mucus, blood and sloughed mucosal tissue, some mixed with pus, fever, heart rate, increased respiratory rate, conjunctival flushing or cyanosis, sunken eye sockets, decreased skin elasticity, decreased urine output, and as the condition worsens, the body temperature drops to normal Temperature below, limbs cold, surface veins collapse, spirit highly depressed even lethargy or coma; and for the prevention or treatment of sheep gastroenteritis, in general, sheep farms often give sheep Western medicine (such as gentamicin, norfloxacin, sulfadiazine, etc.) and health drugs for prevention and treatment, but long-term use of Western medicine and health drugs, viruses and bacteria are very easy to develop drug resistance, so Western medicine and health drugs for the prevention and treatment of sheep gastroenteritis will gradually reduce the efficacy, and cause a large amount of drug residues to mutton, and people will eventually endanger human health. At present, there are also reports on the use of compound Chinese medicine added to feed to prevent sheep gastroenteritis, but many compound Chinese medicine preparations are mixed solutions obtained by decocting a variety of Chinese herbal medicines, there are unclear effective ingredients that play a role, the effect and principle need to be further clarified, and the effect is not stable enough. Therefore, there is an urgent need for a feed additive that can stably and safely prevent mutton from infecting gastroenteritis and promote mutton weight gain. Summary of the invention

[0004] The compound Chinese medicine combination of the present invention can promote the weight gain of mutton sheep and prevent enteritis, solves the problems of drug resistance and drug residue caused by the use of Western medicine in the prior art, and also solves the problem of unclear effective ingredients and unknown contents in the use of compound Chinese medicine in the prior art.

[0005] A feed additive, the compound Chinese medicine combination comprises isatis indigofera extract, glycyrrhizic acid, bacillus subtilis and astragalus polysaccharide.

[0006] The content of the isatis indigo leaf extract is 60-50%.

[0007] The content of glycyrrhizic acid is 30-20%.

[0008] The content of Bacillus subtilis is 10%-5%.

[0009] The content of astragalus polysaccharide is 15%-10%.

[0010] The compound Chinese medicine combination is an oral Chinese medicine preparation.

[0011] The dosage of the additive is 400 g per ton of feed.

[0012] The additive is used in preparing feed for preventing sheep enteritis.

[0013] The additive is used in preparing feed for promoting weight gain of mutton sheep.

[0014] Beneficial Effects

[0015] This feed additive is a compound Chinese medicine preparation with clear ingredients, easy to obtain, and simple preparation. All components are clear effective ingredients extracted from Chinese herbal medicines, and it has a good preventive and therapeutic effect on inflammatory diarrhea that is prone to occur in weaned lambs entering the fattening stage. Folium Isatidis has a good inhibitory effect on a variety of pathogens; glycyrrhizic acid has a significant anti-inflammatory effect, reducing the intestinal pro-inflammatory cytokines IFN-γ and TNF-α, and increasing the content of the anti-inflammatory cytokine IL-10; Bacillus subtilis can regulate intestinal flora, maintain intestinal homeostasis, promote the growth of probiotics, and maintain intestinal health; Astragalus polysaccharide has a good effect on regulating the body's immunity, can promote the increase in the secretion of sIgA antibodies in the body's intestines, and protect the integrity of the intestinal barrier function. Therefore, in order to solve the problem of inflammatory diarrhea caused by intestinal pathogen infection, intestinal flora disorder or intestinal stress caused by weaning, this preparation uses an extract of Isatis indigotica that can inhibit pathogens, supplemented with glycyrrhizic acid that has anti-inflammatory effects and astragalus polysaccharide that regulates immune function, combined with Bacillus subtilis that has the function of regulating intestinal flora. It can effectively reduce the effects of enteritis and diarrhea in fattening lambs and promote the growth of meat sheep, increase the average daily weight gain, and enhance immunity. It functionally replaces the use of antibiotics in feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Figure 2 is a graph showing the results of cytokine content detection, wherein A is the result of IL-1 content detection, and B is the result of IL-12 content detection. DETAILED DESCRIPTION

[0017] Example 1. Preparation of compound Chinese medicine combination.

[0018] The oral compound Chinese medicine combination includes: isatis indigotica extract, glycyrrhizic acid, Bacillus subtilis, and astragalus polysaccharide.

[0019] Usage: Mix 60-50% of isatis leaf extract, 30-20% of glycyrrhizic acid, 10%-5% of bacillus subtilis and 15%-10% of astragalus polysaccharide into 400g of traditional Chinese medicine preparation according to 1 ton of feed for use.

[0020] Example 2. Verification of the effect of the compound Chinese medicine combination on promoting weight gain of mutton sheep.

[0021] Three experimental groups were set up respectively, and the control group was a fattening feed without adding the compound Chinese medicine combination. The formula of the fattening feed is shown in Table 1; the compound Chinese medicine combination of experimental group 1 is 50% of isatis leaf extract, 30% of glycyrrhizic acid, 10% of Bacillus subtilis, and 10% of astragalus polysaccharide, and 400g of the compound Chinese medicine combination is mixed into each ton of fattening feed; the compound Chinese medicine combination of experimental group 2 is 60% of isatis leaf extract, 20% of glycyrrhizic acid, 5% of Bacillus subtilis, and 15% of astragalus polysaccharide, and 400g of the compound Chinese medicine combination is mixed into each ton of fattening feed; each group has 10 mutton sheep of similar weight at 45 days of age, fed for 60 days, free to eat every day, record the feed consumption of each group, measure the weight of mutton sheep every 20 days, and calculate the average daily weight gain, average daily feed intake and feed-to-weight ratio. The results are shown in Table 2. According to Table 2, the average daily weight gain of experimental group 1 and experimental group 2 has increased significantly.

[0022] Table 1

[0023]

[0024]

[0025] The fattening feed provides the following per kilogram of feed: iron 10mg; manganese 135mg; zinc 100mg; cobalt 0.5mg; copper 12.5mg; selenium 0.3mg; iodine 1.5mg; vitamin A 1400IU; vitamin D 500IU; vitamin E 50mg. Metabolizable energy and metabolizable protein are calculated values, and the rest are measured values

[0026] Table 2 Results of the growth performance of mutton sheep

[0027]

[0028]

[0029] Data in the same column with different letters on the shoulders indicate significant differences (P < 0.05); data with the same letters on the shoulders or no letters on the shoulders indicate no significant differences (P > 0.05).

[0030] Example 3. Verification of the effect of the compound Chinese medicine combination on preventing enteritis.

[0031] Three experimental groups were set up respectively, the control group was fattening feed without adding compound Chinese medicine combination; the compound Chinese medicine combination of experimental group 1 was 50% of isatis extract, 30% of glycyrrhizic acid, 10% of bacillus subtilis, and 10% of astragalus polysaccharide, and 400g of compound Chinese medicine combination was mixed into each ton of fattening feed; the compound Chinese medicine combination of experimental group 2 was 60% of isatis extract, 20% of glycyrrhizic acid, 5% of bacillus subtilis, and 15% of astragalus polysaccharide, and 400g of compound Chinese medicine combination was mixed into each ton of fattening feed. Each group had 10 mutton sheep of similar weight at 45 days of age, and the sheep were free to eat. At the same time, the sheep in the control group, experimental group 1, and experimental group 2 were respectively gavaged with 4g / kg of sodium dextran sulfate per day according to their body weight, and the diarrhea of ​​the sheep was observed. When the sheep in the control group had diarrhea, the diarrhea situation and quantity were recorded, and the serum was collected to detect the levels of pro-inflammatory cytokines IL-1 and IL-12. Diarrhea scores include: no diarrhea: the feces are dry and spherical; mild diarrhea: the feces are loose and unformed; severe diarrhea: the feces are sticky around the anus and the stool is loose. As shown in Table 3, the diarrhea of ​​the sheep in test group 1 and test group 2 was significantly improved compared with the control group. Figure 1 It can be seen that compared with the control group, Experimental Group 1 and Experimental Group 2 were able to significantly reduce the levels of proinflammatory cytokines IL-1 and IL-12, indicating that the inflammatory response could be significantly reduced.

[0032] Table 3 Statistics of diarrhea

[0033] Group No diarrhea Mild diarrhea Severe diarrhea Control group 10 Experimental Group 1 8 2 Experimental Group 2 7 3

Claims

1. A feed additive, characterized in that: The additive is a compound Chinese medicine combination, which includes isatis indigo leaf extract, glycyrrhizic acid, bacillus subtilis and astragalus polysaccharide.

2. The additive according to claim 1, characterized in that The content of the isatis indigo leaf extract is 60-50%.

3. The additive according to claim 1, characterized in that The content of glycyrrhizic acid is 30-20%.

4. The additive according to claim 1, characterized in that The content of Bacillus subtilis is 10%-5%.

5. The additive according to claim 1, characterized in that The content of astragalus polysaccharide is 15%-10%.

6. The additive according to claim 1, characterized in that The compound Chinese medicine combination is an oral Chinese medicine preparation.

7. The additive according to claim 1, characterized in that The dosage of the additive is 400 g per ton of feed.

8. Use of the additive according to claim 1 in preparing feed for preventing sheep enteritis.

9. Use of the additive according to claim 1 in preparing feed for promoting weight gain of mutton sheep.

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