PCR amplification gene detection device

Abstract

The invention relates to the technical field of gene detection, in particular to a PCR amplification gene detection device which comprises a lower structure, an upper structure and a hinge structure. The lower structure comprises an input button, a display screen, a master control host, a heating device, a water cooling circulation pipeline and a sample bearing groove; the buttons and the display screen are arranged on the human front view surface of the lower structure shell; the master control host comprises a signal transmission module, a temperature control module and an operation instruction feedback module, and is arranged in a built-in position above the lower structure base; the heating device is a heating plate and is arranged below the sample bearing groove; the water cooling circulation pipeline is a parallel liquid pipeline penetrating through the lower structure; the upper structure comprises an illumination and fluorescence integrated plate and a program adjusting turntable; the illumination and fluorescence integrated plate is arranged at the contact position of the bottom end of the upper structure and the lower structure; and the program adjusting turntable can perform signal transmission with the master control host and is arranged right above the upper structure.

Description

Technical Field

[0001] The invention relates to the field of gene detection, in particular to a PCR amplification gene detection device. Background Art

[0002] PCR technology is a method commonly used in gene detection and disease diagnosis. However, existing PCR amplification gene detection devices have some shortcomings, such as high price and inconvenient operation. Summary of the invention

[0003] According to an embodiment of the present invention, a PCR amplification gene detection device is characterized in that: the PCR amplification gene detection device includes: a lower structure, an upper structure, and a hinge structure; wherein the lower structure includes: an input button and a display screen, a master control host, a heating device, a water-cooled circulation pipe, and a sample holding tank; the button and the display screen are arranged on the front surface of the lower structure shell; the master control host includes: a signal transmission module, a temperature control module, and an operation instruction feedback module, which are arranged in a built-in position above the lower structure base; the heating device is a heating plate, which is arranged below the sample holding tank; the water-cooled circulation pipe is a parallel liquid pipe running through the lower structure; the upper structure includes: an illumination, a fluorescent integrated plate, and a program adjustment dial; the illumination, the fluorescent integrated plate are arranged at a contact position with the bottom of the upper structure and the lower structure; the program adjustment dial and the master control host can transmit signals, and are arranged directly above the upper structure.

[0004] Furthermore, the signal transmission module included in the master control host is connected to the heating device, lighting, fluorescent integrated panel and program adjustment dial through signal lines.

[0005] Furthermore, the temperature control module and the operation instruction feedback module included in the master control host perform signal adjustment through the master control host analyzing parameters and transmit the adjusted signals to each component that needs to be changed.

[0006] Furthermore, the temperature control module ensures that the temperature cycle of the PCR reaction is accurate and reliable by monitoring and adjusting the temperature of the heating device in the lower structure.

[0007] Furthermore, the operation instruction feedback module provides the user with real-time feedback information on the experimental progress, parameters and status through the operation instructions input by the user.

[0008] Furthermore, the surface of the heating plate has multiple heating areas to meet different reaction temperature requirements.

[0009] Furthermore, the liquid pipeline further includes a heat exchange device for quickly adjusting and maintaining the temperature of the water-cooled circulating liquid.

[0010] Furthermore, the illumination and fluorescence integrated plate has illumination sources with multiple wavelengths and a fluorescence detection unit for detecting and analyzing multi-channel fluorescence signals.

[0011] Furthermore, the program adjustment dial is rotatably connected to the master control host for selecting and switching different PCR programs and experimental settings.

[0012] Furthermore, the maximum opening and closing angle of the hinge is 85°. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A schematic diagram of various components of a PCR amplification gene detection device provided by the present invention;

[0014] Figure 2 A schematic diagram of a PCR amplification gene detection device provided by the present invention in an open (non-working) state;

[0015] Figure 3 A schematic diagram of a PCR amplification gene detection device provided by the present invention in a closed (working) state;

[0016] Figure 4 This is an engineering diagram of a PCR amplification gene detection device provided by the present invention.

[0017] Legend: 1. Lower structure; 2. Upper structure; 3. Hinge structure; 4. Input button and display screen; 5. Master control host; 6. Heating device; 7. Water-cooling circulation pipeline; 8. Sample holding tank; 9. Lighting and fluorescence integrated board; 10. Program adjustment dial. Specific implementation plan

[0018] The present invention is described in detail below with reference to the examples. The examples are only preferred embodiments of the present invention and are not intended to limit the present invention.

[0019] See also Figure 1 The present invention is a PCR amplification gene detection device, including a lower structure, an upper structure, and a hinge structure; wherein the lower structure includes: input buttons and a display screen, a master control host, a heating device, a water-cooled circulation pipeline, and a sample holding tank; the upper structure includes: lighting, a fluorescent integrated board, and a program adjustment dial.

[0020] In this embodiment, as a preferred solution of this example, the assembly and function of the lower structure are as follows;

[0021] The input button and display screen are located on the front surface of the lower structure shell and are connected to the main control host through a connecting circuit for the user to set the experimental parameters.

[0022] The main control host includes a signal transmission module, a temperature control module and an operation instruction feedback module. The signal transmission module is connected to the lighting, fluorescent integrated board and program adjustment dial in the upper structure through wires to achieve signal transmission. The temperature control module monitors the temperature of the heating device through sensors and adjusts the heating power through the control circuit to maintain the required PCR reaction temperature cycle. The operation instruction feedback module receives the operation instructions input by the user and provides the user with real-time experimental progress, parameters and status information through the display screen.

[0023] The heating device is a heating plate with multiple heating zones, and the temperature of each zone can be independently controlled to meet the temperature requirements of different PCR reactions.

[0024] The water-cooling circulation pipeline runs through the lower structure, including a heat exchange device, which realizes the circulation and temperature regulation of the water-cooling circulating fluid through a pump and a control valve. The heat exchange device can quickly adjust the temperature of the water-cooling circulating fluid according to temperature feedback to ensure the stability and reliability of the PCR reaction.

[0025] The sample holding tank is located above the heating device and is used to hold the test tubes or microplates for the reaction.

[0026] In this embodiment, as a preferred solution of this example, the assembly and function of the upper structure are as follows.

[0027] The upper structure includes an integrated lighting and fluorescence board and a program adjustment dial. The integrated lighting and fluorescence board is located at the bottom of the upper structure and is in close contact with the lower structure. The lighting source and fluorescence detection unit therein can generate and capture multi-channel fluorescence signals.

[0028] The program adjustment dial is located just above the upper structure and can be rotatably connected to the master control host and communicate with the master control host through the signal transmission module. Users can select the desired PCR program and experimental settings through the dial.

[0029] In this embodiment, as a preferred solution of this example, the operating steps of the device are as follows.

[0030] 1. Carry out equipment preparation work.

[0031] Place the PCR amplification gene detection device on a stable work surface and connect the power cord.

[0032] Open the lower structure shell of the device and ensure that the heating device, sample holding tank and other components are clean and intact.

[0033] 2. Set the experimental parameters.

[0034] Pressing the lower structure's enter button starts the unit and displays the main interface on the display.

[0035] Use the operation interface on the display to set the required experimental parameters, such as target gene, primer concentration, reaction system, etc. After confirming the parameter settings, press the confirmation button.

[0036] 3. Perform sample loading.

[0037] Open the sample holding tank cover of the lower structure, place the test tube or microplate containing the PCR reaction mixture in the sample holding tank, and ensure that the sample is placed correctly.

[0038] 4. Close the sample holding chamber cover.

[0039] Gently close the sample holder cover to ensure a tight seal.

[0040] 5. Start the experiment.

[0041] In the operation interface on the display screen, click the "Start Experiment" button and the device will start the PCR amplification reaction according to the preset temperature cycle parameters.

[0042] 6. Temperature cycle.

[0043] The master control host controls the heating device to perform temperature cycling according to the preset temperature cycle parameters. The temperature control module monitors the temperature in the sample holding tank and maintains the required reaction temperature by controlling the heating power of the heating plate.

[0044] 7. Fluorescence signal detection.

[0045] At different stages of the PCR reaction, the illumination and fluorescence integrated plate generates illumination light sources of different wavelengths to excite the fluorescent markers in the PCR products. The fluorescence detection unit captures the fluorescent signal, converts it into an electrical signal and transmits it to the master control host.

[0046] 8. Experimental progress monitoring.

[0047] The operation instruction feedback module monitors the experimental process in real time and displays it on the display screen. Users can check the experimental progress, temperature curve and real-time fluorescence signal at any time.

[0048] 9. Analysis of experimental results.

[0049] After the experiment is over, the display will show the fluorescence signal curve of PCR amplification and the results of fluorescence quantitative analysis. Users can perform gene detection, quantitative analysis or other related research based on the changes in the fluorescence signal.

[0050] 10. Turn off the device.

[0051] After the experiment is completed, click the "Stop Experiment" button on the display screen to stop the temperature cycle and signal detection. Disconnect the power supply and wait for the device to cool down before opening the lower structure shell and taking out the reaction tube or microplate.

Claims

1. A PCR amplification gene detection device, characterized in that: The PCR amplification gene detection device comprises: a lower structure, an upper structure, and a hinge structure; wherein the lower structure comprises: an input button and a display screen, a master control host, a heating device, a water-cooling circulation pipeline, and a sample holding tank; wherein the button and the display screen are arranged on the front view surface of the lower structure shell; the master control host comprises: a signal transmission module, a temperature control module, and an operation instruction feedback module, which are arranged above the base of the lower structure and positioned inside; the heating device is a heating plate, which is arranged below the sample holding tank; the water-cooling circulation pipeline is a parallel liquid pipeline running through the lower structure; the upper structure comprises: an illumination, a fluorescent integrated plate, and a program adjustment dial; wherein the illumination, the fluorescent integrated plate is arranged at a contact position with the lower structure at the bottom end of the upper structure; the program adjustment dial and the master control host can transmit signals, and are arranged directly above the upper structure. .

2. A PCR amplification gene detection device according to claim 1, characterized in that: The signal transmission module included in the master control host is connected to the heating device, lighting, fluorescent integrated board and program adjustment dial through signal lines.

3. A PCR amplification gene detection device according to claim 1, characterized in that: The temperature control module and the operation instruction feedback module included in the master control host perform signal adjustment through the master control host analyzing parameters and transmit the adjusted signals to each component that needs to be changed.

4. A PCR amplification gene detection device according to claim 1, characterized in that: The temperature control module ensures that the temperature cycle of the PCR reaction is accurate and reliable by monitoring and adjusting the temperature of the heating device in the lower structure.

5. A PCR amplification gene detection device according to claim 1, characterized in that: The operation instruction feedback module provides the user with real-time feedback information on the experimental process, parameters and status through the operation instructions input by the user.

6. A PCR amplification gene detection device according to claim 1, characterized in that: The surface of the heating plate has multiple heating areas to meet different reaction temperature requirements.

7. A PCR amplification gene detection device according to claim 1, characterized in that: The liquid pipeline further includes a heat exchange device for quickly adjusting and maintaining the temperature of the water-cooled circulating liquid.

8. A PCR amplification gene detection device according to claim 1, characterized in that: The illumination and fluorescence integrated plate has illumination sources with multiple wavelengths and a fluorescence detection unit, and is used for detecting and analyzing multi-channel fluorescence signals.

9. A PCR amplification gene detection device according to claim 1, characterized in that: The program adjustment dial is rotatably connected to the master control host and is used to select and switch different PCR programs and experimental settings.

10. A PCR amplification gene detection device according to claim 1, characterized in that: The maximum opening and closing angle of the hinge is 85°.

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