Kit for detecting bovine tuberculosis r interferon antibody and detection method thereof

Abstract

The invention discloses a kit for bovine tuberculosis r interferon antibody detection and a detection method thereof.The kit comprises a kit body, the kit body is internally provided with a film sealing assembly, the film sealing assembly comprises a lifting plate slidably connected with the inner wall of the kit body, the top of the lifting plate is provided with a first telescopic rod and a supporting table, and the top of the supporting table is provided with a containing frame; an elisa plate is detachably mounted in the placement frame, the outer wall of the first lead screw is sleeved with a moving frame, the bottom wall of the moving frame is connected with a first pressing frame, and the roll film is located between the first pressing frame and the elisa plate. By arranging the film sealing assembly, after a reagent is added into an elisa plate, a roll film is extruded above the elisa plate, the purpose of automatically sealing the elisa plate with the film is achieved, the phenomenon that when the elisa plate moves, the reagent in the elisa plate leaks out, and consequently the antibody detection result is inaccurate is avoided, and by arranging the adjusting frame, the first pressing frame, the second pressing frame, the pressing plate and the roll film, the detection accuracy is improved. The length of the used roll film can be conveniently controlled according to the number of the elisa plates, and the phenomenon of roll film waste is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of antibody detection, in particular to a kit for detecting bovine tuberculosis interferon-R antibodies and a detection method thereof. Background Art

[0002] Bovine tuberculosis is a chronic wasting zoonosis caused by Mycobacterium bovis, and is classified as a Class B animal infectious disease by the International Organization for Animal Health. In order to prevent the spread of bovine tuberculosis in cattle herds, it is necessary to test the cattle herds for bovine tuberculosis antibodies and isolate or kill the sick cattle. The current method for detecting bovine tuberculosis is to use an r-interferon antibody detection kit for r-interferon antibody detection. However, when performing antibody detection, the existing kit requires manual sealing and peeling of the ELISA plate, and the sealing film cannot be automatically adjusted according to the actual number of ELISA plates used, which increases the cost of antibody detection and reduces detection efficiency.

[0003] The defects of existing antibody detection kits are: The patent document JP2008261840A mainly considers the sensitivity of the antibody detection kit when performing antibody detection, but does not consider how to seal and remove the membrane of the ELISA plate and adjust the size of the membrane used; The patent document JP2006184295A discusses how to improve the quantitative certainty and safety of the kit, but does not consider how to facilitate the washing of the ELISA plate. Patent document CN1982439A mainly considers how to improve the specificity and sensitivity of the test kit, but does not consider how to automatically dry the ELISA plate; Patent document CN204389503U mainly improves the detection efficiency of the test kit by adjusting the placement of reagents used in the detection process. It does not take into account the need to use more equipment and frequently adjust the position of the ELISA plate during the detection process, which can easily reduce the detection efficiency and accuracy. Summary of the invention

[0004] The object of the present invention is to provide a kit for detecting bovine tuberculosis interferon-γ antibodies and a detection method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above object, the present invention provides the following technical scheme: a kit for detecting bovine tuberculosis interferon-r antibodies, comprising a box body and a sealing film assembly, wherein the box body is provided with a sealing film assembly inside, and the sealing film assembly comprises a lifting plate slidably connected to the inner wall of the box body, a telescopic rod 1 and a support platform are installed on the top of the lifting plate, and the telescopic rod 1 is located on both sides of the support platform, and a placement frame is provided on the top of the support platform, and an ELISA plate is detachably installed in the placement frame; A cross frame is installed on the top of the telescopic rod one, and a micro servo motor one is arranged inside the cross frame, and a screw rod one is connected to the output end of the micro servo motor one, and a mobile frame is arranged on the outer wall of the screw rod one, and the mobile frame is located above the ELISA plate, and the top wall of the mobile frame is connected to the placement frame, and a winding motor is arranged on the back of the placement frame, and the output end of the winding motor is connected to the winding frame, and a linear motor unit is arranged on the top wall of the mobile frame, and the linear motor unit includes a linear guide rail installed on the top wall of the mobile frame, and the outer wall of the linear guide rail is slidably connected with the linear motor, and the output end of the linear motor is connected to the mounting platform, and an adjustment frame is installed at the bottom of the mounting platform, and the adjustment frame is located on the right side of the mobile frame, and an unwinding motor is arranged on the back of the adjustment frame, and the output end of the unwinding motor is connected to the unwinding frame, and a film roll is arranged on the outer wall of the unwinding frame; The bottom wall of the movable frame is connected to a pressing frame 1, the outer wall of the pressing frame 1 is slidably connected to a pressing frame 2, and the pressing frame 1 and the pressing frame 2 are located between the fixed frame and the adjustment frame, the film roll is located between the pressing frame 1 and the ELISA plate, the top wall of the pressing frame 1 is installed with a spring 1, the bottom end of the spring 1 is connected to a pressing plate, and the pressing plate is embedded and connected to the inner wall of the pressing frame 1, the inner wall of the pressing frame 1 is embedded and installed with an electromagnet, and the electromagnet is located below the pressing plate, and a connecting rod is installed on the top of the pressing frame 2, and the connecting rod is connected to the outer wall of the adjustment frame.

[0006] Preferably, a second telescopic rod is embedded and installed in the inner wall of the support platform, an output end of the second telescopic rod is connected to a clamping frame, and the clamping frame is used to fix the placement frame.

[0007] Preferably, a partition plate is installed on the inner wall of the box body 1, the lifting plate is located on the left side of the partition plate, a waste liquid tank is provided on the right side of the partition plate, and a washing liquid tank is provided on the right side of the waste liquid tank. A mounting groove is opened on the left outer wall of the partition plate, and a rack 1 is embedded and installed on the inner wall of the mounting groove. A rack 2 is installed on the top of the lifting plate, and the rack 2 is located on the front and rear sides of the support platform, and the rack 2 and the rack 1 are located on the same straight line. The outer wall of the rack 1 is slidably connected with a sliding frame, and the top of the sliding frame is installed with a rack 3. The inner wall of the sliding frame is installed with a driving motor, and the outer wall of the output end of the driving motor is sleeved with a gear, and the gear is connected with the rack 1, the rack 2 and the rack Three are meshed, a vertical rod is installed on the top of rack three, a blocking block is installed on the top of the vertical rod, and the blocking block quickly passes through the outer wall of the partition plate, a horizontal plate is installed on the right side of the partition plate, and a telescopic rod three is installed on the bottom of the horizontal plate. The output end of telescopic rod three is connected to a washing unit, and the washing unit includes an L-shaped bracket connected to the output end of telescopic rod three, and the top of the L-shaped bracket is connected to a washing nozzle. A negative pressure pump and a delivery pump are arranged on the top of the waste liquid tank, and delivery pipes are arranged on the input and output ends of the delivery pump and the negative pressure pump. The washing nozzle is connected to the washing liquid tank through the delivery pipe and the delivery pump, and the washing nozzle is connected to the waste liquid tank through the delivery pipe and the negative pressure pump.

[0008] Preferably, a second micro servo motor is installed on the bottom wall of the box body, the output end of the second micro servo motor is connected to a second screw rod, and the lifting plate is sleeved on the outer wall of the second screw rod, and the second screw rod is located at the front and rear sides of the second rack.

[0009] Preferably, a flip plate is provided through the top of the lifting plate, a flip motor is installed on the inner wall of the lifting plate, and the output end of the flip motor is connected to the outer wall of the flip plate, a fixed platform is installed on the top of the flip plate, and the support platform is sleeved on the outer wall of the fixed platform, a micro servo motor three is installed inside the fixed platform, and a rotating frame is sleeved on the outer wall of the output end of the micro servo motor three, a cylindrical rod is installed on the outer wall of the rotating frame, an arc frame is installed on the top wall of the support platform, and the cylindrical rod is embedded and connected to the inner wall of the arc frame, a pull-out frame is slidably connected to the inner wall of the box body, and the pull-out frame is located below the lifting plate, absorbent tissue paper is detachably placed on the inner wall of the pull-out frame, a telescopic rod four is embedded and installed on the bottom wall of the box body, a push block is connected to the output end of the telescopic rod four, and the push block is located below the absorbent tissue paper.

[0010] Preferably, the pull-out frame includes two groups of long rods, and the left ends of the two groups of long rods are hinged by a rotating column. A second spring is installed on the inner wall of the pull-out frame, and the second spring is located between the long rods on both sides. A ball is embedded in the inner wall of the side where the two groups of long rods are close to each other, and the ball is in contact with the absorbent cotton paper.

[0011] Preferably, electric heating plates are installed on the inner walls of the front and rear sides of the box body.

[0012] Preferably, a pressing frame is embedded and connected to the top of the placement frame, and a fixing pin is installed on the outer wall of one side of the pressing frame close to the top of the placement frame, and the fixing pin passes through the top of the ELISA plate.

[0013] A method for detecting bovine tuberculosis r-interferon antibody, the method for detecting bovine tuberculosis r-interferon antibody is as follows: S1. Open the box, take out the reagents and ELISA plates used in the antibody detection process placed in the box, and place the ELISA plates in the placement frame according to the required amount; S2, the lifting plate drives the support platform to move upward until the support platform leaks out of the top of the box body, and the micro servo motor 2 is started, so that the screw rod 2 drives the moving frame to move backward, leaking out the support platform and installing the placement frame; S3, adding antibody detection standard, diluent, sample, and enzyme-labeled conjugate reagent into the ELISA plate in sequence, resetting the mobile rack, and then sealing the top of the ELISA plate by squeezing the roll film through the first and second pressing plates; S4, moving the ELISA plate into the box body for incubation, washing the ELISA plate through the washing unit after incubation, and wiping the ELISA plate dry; S5. Perform S1 again, add the color developing solution and the stop solution to the ELISA plate, take out the ELISA plate after incubation, and read the data using an ELISA reader.

[0014] Preferably, in S3, the following steps are also included: S31, adjusting the second pressing frame and the adjustment frame position according to the number of groups of ELISA plates used, and controlling the unrolling length of the film; In S4, the following steps are also included: S41. When drying the ELISA plate, firstly, the support table is turned over by the turning plate, the ELISA plate is rotated so that the opening faces downward, and then the absorbent cotton paper is pushed into the ELISA plate by the pushing block to absorb the residual moisture.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention is equipped with a film sealing assembly, including a movable frame, a film roll, a pressing frame 1 and a pressing frame 2. After the reagent is added into the ELISA plate, the telescopic rod 1 is shortened, and the movable frame is pulled downward, so that the pressing frame 1, the pressing frame 2 and the pressing plate squeeze the film roll on the top of the ELISA plate, thereby achieving the purpose of automatically sealing the ELISA plate, avoiding the leakage of the internal reagent when the ELISA plate is moved, resulting in inaccurate antibody detection results. At the same time, by arranging the adjustment frame, the pressing frame 1, the pressing frame 2, the pressing plate and the film roll, it is convenient to control the length of the film roll used according to the number of ELISA plates, thereby avoiding the phenomenon of film roll waste.

[0016] 2. The present invention is equipped with racks 1, 2 and 3. After the incubation is completed, the driving motor drives the gears to rotate, so that rack 3 drives the blocking block and the washing unit to move to the left above the ELISA plate. By setting the washing unit, there is no need to take out the ELISA plate after incubation and then wash it, which reduces the equipment used in the antibody detection process and further improves the antibody detection efficiency.

[0017] 3. The present invention is equipped with a flip plate, which rotates the ELISA plate to a state with the opening facing downward after washing, and drives the rotating frame to rotate clockwise and counterclockwise through the micro servo motor, and drives the support platform to shake up and down through the rotating plate, so as to accelerate the separation of the washing liquid from the inside of the ELISA plate, and push the absorbent cotton paper into the ELISA plate through the push block, so as to achieve the purpose of wiping the ELISA plate, thereby avoiding the presence of washing liquid in the ELISA plate and thus causing adverse effects on the antibody detection results.

[0018] 4. The present invention is equipped with an electric heating plate for incubating the reagents in the ELISA plate inside the box body. Through the arrangement of the electric heating plate, the sealing assembly and the washing unit, the box body can achieve the purpose of sealing, washing, drying and incubating the ELISA plate in an integrated manner, which is beneficial to speeding up the antibody detection efficiency and avoiding repeated taking out and putting in of the ELISA plate, reducing the possibility of contamination of the ELISA plate and reagents, and is beneficial to improving the accuracy of the antibody detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 It is a schematic diagram of the structure of the film sealing assembly of the present invention; Figure 3 It is a schematic diagram of the structure of the mobile rack of the present invention; Figure 4 It is a schematic diagram of the structure of the fixing frame of the present invention; Figure 5 It is a structural schematic diagram of a pressing frame of the present invention; Figure 6 It is a schematic diagram of the pressing plate structure of the present invention; Figure 7 It is a schematic diagram of the structure of the partition plate of the present invention; Figure 8 It is a schematic diagram of the structure of a rack of the present invention; Fig. 9 It is a schematic diagram of the flip plate structure of the present invention; Fig.10 It is a schematic diagram of the structure of the pull-out frame of the present invention; Fig.11 It is a schematic diagram of the placement frame structure of the present invention.

[0020] In the figure: 1, box body; 2, lifting plate; 3, support table; 4, placement frame; 5, ELISA plate; 6, telescopic rod 1; 7, horizontal frame; 8, micro servo motor 1; 9, screw rod 1; 10, moving frame; 11, fixed frame; 12, winding motor; 13, winding frame; 14, linear motor unit; 15, adjustment frame; 16, unwinding motor; 17, unwinding frame; 18, film roll; 19, pressing frame 1; 20, pressing frame 2; 21, connecting rod; 22, spring 1; 23, pressing plate; 24, electromagnet; 25, micro servo motor 2; 26, screw rod 2; 27, telescopic rod 2; 28, clamping frame; 29, partition plate; 30, washing Washing liquid tank; 31. Waste liquid tank; 32. Mounting slot; 33. Rack one; 34. Rack two; 35. Sliding frame; 36. Rack three; 37. Driving motor; 38. Gear; 39. Vertical rod; 40. Blocking block; 41. Horizontal plate; 42. Telescopic rod three; 43. Washing unit; 44. Turning plate; 45. Turning motor; 46. Fixed table; 47. Micro servo motor three; 48. Rotating frame; 49. Arc frame; 50. Pull-out frame; 51. Absorbent tissue paper; 52. Telescopic rod four; 53. Push block; 54. Long rod; 55. Spring two; 56. Ball bearing; 57. Electric heating plate; 58. Pressing frame; 60. Fixing pin. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0023] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0024] See also Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 , an embodiment provided by the present invention: a kit for detecting bovine tuberculosis r interferon antibody, comprising a box body 1 and a sealing film assembly, the box body 1 is provided with a sealing film assembly, the sealing film assembly comprises a lifting plate 2 slidably connected to the inner wall of the box body 1, the left side of the lifting plate 2 is fitted with the left inner wall of the box body 1, the left outer wall of the lifting plate 2 is provided with a protrusion, and the protrusion is embedded and connected to the left inner wall of the box body 1, the right outer wall of the lifting plate 2 is fitted with the left outer wall of the partition plate 29, and there is a gap between the front and rear outer walls of the lifting plate 2 and the front and rear inner walls of the box body 1, a telescopic rod 6 and a support platform 3 are installed on the top of the outer lifting plate 2, and the telescopic rod 6 is located on both sides of the support platform 3, a placement frame 4 is provided on the top of the support platform 3, an ELISA plate 5 is detachably installed in the placement frame 4, a micro servo motor 25 is installed on the bottom wall of the box body 1, a screw rod 26 is connected to the output end of the micro servo motor 25, and the lifting plate 2 is sleeved on the outer wall of the screw rod 26; A cross frame 7 is installed on the top of the telescopic rod 6, and the cross frame 7 is located on the left and right sides of the ELISA plate 5. A micro servo motor 8 is arranged inside the cross frame 7. The output end of the micro servo motor 8 is connected to a screw rod 9. The outer wall of the screw rod 9 is provided with a mobile frame 10, and the mobile frame 10 is located above the ELISA plate 5. The top wall of the mobile frame 10 is connected to a placement frame, and the placement frame is located at the upper left of the ELISA plate 5. A winding motor 12 is arranged on the back of the placement frame, and the output end of the winding motor 12 is connected to the winding frame 13. A guide roller is installed on the inner wall of the placement frame, and the guide roller is located at the winding frame 13. On the right side of the movable frame 10, a linear motor unit 14 is arranged on the top wall of the movable frame 10. The linear motor unit 14 comprises a linear guide rail mounted on the top wall of the movable frame 10. The outer wall of the linear guide rail is slidably connected with a linear motor. The output end of the linear motor is connected with a mounting platform. An adjustment frame 15 is installed at the bottom of the mounting platform. The adjustment frame 15 is located on the right side of the movable frame 10. The adjustment frame 15 is located on the upper right side of the ELISA plate 5. An unwinding motor 16 is arranged on the back side of the adjustment frame 15. The output end of the unwinding motor 16 is connected with an unwinding frame 17. A guide roller is installed on the inner wall of the adjustment frame 15. The guide roller Located on the left side of the unwinding frame 17, the outer wall of the unwinding frame 17 is provided with a roll film 18, the left end of the roll film 18 is rolled up by the winding frame 13, and the guide roller is used to guide the roll film 18 to change the extension direction of the roll film 18, the bottom wall of the movable frame 10 is connected with a pressing frame 19, the outer wall of the pressing frame 19 is slidably connected with a pressing frame 20, the bottom of the pressing frame 20 is a plane, and the pressing frame 19 and the pressing frame 20 are located between the fixed frame 11 and the adjustment frame 15, the roll film 18 is located between the pressing frame 19 and the ELISA plate 5, and the bottom of the pressing frame 20 is in contact with the top of the roll film 18, and the pressing frame A spring 22 is installed on the top wall of a press frame 19, and a pressure plate 23 is connected to the bottom end of the spring 22, and the pressure plate 23 is embedded and connected to the inner wall of the press frame 19. An electromagnet 24 is embedded and installed on the inner wall of the press frame 19, and the electromagnet 24 is located below the pressure plate 23. When the electromagnet 24 is energized, a magnetic attraction is generated to adsorb the pressure plate 23. A connecting rod 21 is installed on the top of the press frame 20, and the connecting rod 21 is connected to the outer wall of the adjustment frame 15. A sliding rod is installed on the top of the press frame 20, and the top end of the sliding rod is embedded and connected to the top wall of the lifting frame, and the sliding rod is located on the front and rear sides of the linear guide rail.

[0025] Further, the micro servo motor 25 drives the screw rod 26 to rotate, so that the lifting plate 2 drives the support platform 3 to move upward, and the support platform 3 is exposed from the top of the box body 1. The telescopic rod 6 is extended, and the horizontal frame 7 is pushed to drive the movable frame 10 to move upward, so that the film roll 18 moves upward. Then, the micro servo motor 8 is started again to drive the screw rod 9 to rotate, so that the movable frame 10 moves backward along the top of the horizontal frame 7 to expose the support platform 3, and the placement frame 4 with the required number of ELISA plates 5 is placed on the top of the support platform 3, and the clamping frame 2 is driven by the telescopic rod 27. 8 moves, fixes the placement frame 4, drives the linear motor unit 14 to drive the adjustment frame 15 to move to the left according to the number of installed groups of the ELISA plate 5, and the adjustment frame 15 pushes the pressing frame 20 to move to the left through the connecting rod 21, and at the same time, the unwinding motor 16 drives the unwinding frame 17 to rotate clockwise to roll up the unused film roll 18, and the adjustment frame 15, the pressing frame 20 and the winding frame 13 operate synchronously, which is conducive to keeping the film roll 18 in a flat state, so that the film roll 18 is always in contact with the bottom of the pressing frame 20. When the pressing frame 20 moves to the left, the pressing frame 11 9, the electromagnet 24 at the corresponding position is disconnected, the pressing plate 23 loses the adsorption force of the electromagnet 24, and under the pull of the spring 1 22, the pressing plate 23 is retracted into the pressing frame 19, thereby keeping the pressing frame 20 able to move to the left, and the bottom of the pressing frame 20 and the bottom of the pressing plate 23 located on the left side of the pressing frame 20 are located in the same plane. After the adjustment frame 15, the pressing frame 20 and the film roll 18 are adjusted, the micro servo motor 18 is reversed, driving the screw rod 19 to reverse, and the moving frame 10 drives the film roll 18 to move forward to the top of the placement frame 4, and then The telescopic rod 16 is shortened, and the movable frame 10 is pulled downward, so that the pressing frame 19, the pressing frame 20 and the pressing plate 23 squeeze the roll film 18 above the ELISA plate 5, so as to achieve the purpose of automatically sealing the ELISA plate 5, and avoid the leakage of the internal reagent when the ELISA plate 5 is moved, resulting in inaccurate antibody detection results. At the same time, by setting the adjustment frame 15, the pressing frame 19, the pressing frame 20, the pressing plate 23 and the roll film 18, it is convenient to control the length of the roll film 18 used according to the number of ELISA plates 5, and avoid the phenomenon of wasting the roll film 18; When peeling off the film, the telescopic rod 6 pushes the movable frame 10 to move upward, so that the rolled film 18 leaves the surface of the ELISA plate 5, and the winding motor 12 drives the winding frame 13 to rotate counterclockwise, and the unwinding motor 16 drives the unwinding frame 17 to rotate counterclockwise to roll up the used rolled film 18, thereby achieving the purpose of automatic peeling off and automatic replacement of the rolled film 18, which improves the convenience of use of the test kit and the efficiency of antibody detection to a certain extent.

[0026] See also Figure 7 and Figure 8An embodiment of the present invention is a kit for detecting bovine tuberculosis interferon-r antibodies, comprising a box body 1, an inner wall of which is installed a partition plate 29, a lifting plate 2 is located on the left side of the partition plate 29, a waste liquid tank 31 is arranged on the right side of the partition plate 29, a washing liquid tank 30 is arranged on the right side of the waste liquid tank 31, and the washing liquid tank 30 is used to hold washing liquid for washing the ELISA plate 5, an installation groove 32 is opened on the left outer wall of the partition plate 29, and a rack 33 is embedded in the inner wall of the installation groove 32, and the lifting plate 2 is located on the left side of the partition plate 29. 2 is provided with a second rack 34 at the top, the second rack 34 is located at the front and rear sides of the support platform 3, and the second rack 34 and the first rack 33 are located on the same straight line, the outer wall of the first rack 33 is slidably connected with a sliding frame 35, the top of the sliding frame 35 is provided with a third rack 36, and the teeth of the third rack 36 face downward, the inner wall of the sliding frame 35 is provided with a driving motor 37, the outer wall of the output end of the driving motor 37 is sleeved with a gear 38, and the gear 38 is meshed with the first rack 33, the second rack 34 and the third rack 36, when the sliding frame 35 is in motion, the gear 38 is engaged with the first rack 33, the second rack 34 and the third rack 36. When the movable frame 35 is slidably connected to the outer wall of the rack 1 33, the gear 38 is meshed with the rack 1 33 and the rack 3 36 at the same time. When the sliding frame 35 is slidably connected to the outer wall of the rack 2 34, the gear 38 is meshed with the rack 2 34 and the rack 3 36 at the same time. A vertical rod 39 is installed on the top of the rack 36, and the vertical rod 39 is located in the installation groove 32. A blocking block 40 is installed on the top of the vertical rod 39, and the blocking block 40 passes through the outer wall of the partition plate 29. A horizontal plate 41 is installed on the right side of the partition plate 29. The horizontal plate 41 A telescopic rod 3 42 is installed at the bottom, and a washing unit 43 is connected to the output end of the telescopic rod 3 42. The washing unit 43 includes an L-shaped bracket connected to the output end of the telescopic rod 3 42, and a washing nozzle is connected to the top of the L-shaped bracket. A negative pressure pump and a delivery pump are arranged on the top of the waste liquid tank 31, and delivery pipes are arranged at the input and output ends of the delivery pump and the negative pressure pump. The washing nozzle is connected to the washing liquid tank 30 through the delivery pipe and the delivery pump, and the washing nozzle is connected to the waste liquid tank 31 through the delivery pipe and the negative pressure pump.

[0027] Further, the micro servo motor 25 drives the screw rod 26 to rotate, so that the lifting plate 2 drives the ELISA plate 5 to move downward to the inside of the box body 1 for incubation. At this time, the rack 2 34 on the top of the lifting plate 2 is aligned with the rack 1 33 in the mounting groove 32. After the incubation is completed, the movable frame 10 moves upward, so that the film roll 18 leaves the top of the ELISA plate 5, and the driving motor 37 is started to drive the gear 38 to rotate. The gear 38 moves along the top of the rack 1 33 to the top of the rack 2 34, and the gear 38 is meshed with the rack 36. Under the restriction of the sliding frame 35, the rack 36 moves to the left in a direction parallel to the rack 1 33 and the rack 2 34. The rack 36 drives the blocking block 40 to move to the left through the vertical rod 39. The blocking block 40 drives the horizontal plate 41 and the washing unit 43 to move to the left above the ELISA plate 5. When the washing nozzle in the washing unit 43 moves to the top of the ELISA plate 5, the telescopic rod 3 42 pushes the washing unit 4 3 moves downward, extends the washing nozzle into the ELISA plate 5, extracts the reagent in the ELISA plate 5 into the waste liquid tank 31 through the delivery pipe and the negative pressure pump, and then injects the washing liquid in the washing liquid tank 30 into the ELISA plate 5 through the delivery pump and the delivery pipe through the washing nozzle, soaks and washes the ELISA plate 5, and the used washing liquid is then extracted into the waste liquid tank 31 through the negative pressure pump and the delivery pipe, and the washing liquid is repeatedly injected 5 times to fully wash the ELISA plate 5. After the washing is completed, the driving motor 37 rotates in the opposite direction, so that the rack three 36 drives the blocking block 40 to move rightward through the vertical rod 39, so that the washing unit 43 returns to the right side of the partition plate 29, and the blocking block 40 is embedded in the partition plate 29 to seal the partition plate 29. By setting the washing unit 43, there is no need to take out the ELISA plate 5 after incubation and then wash it, which reduces the equipment used in the antibody detection process and further improves the antibody detection efficiency.

[0028] See also Fig. 9 and Fig.10, an embodiment of the present invention: a kit for detecting bovine tuberculosis r-interferon antibody, comprising a lifting plate 2 having a flip plate 44 disposed through the top, the flip plate 44 being located in the middle of the lifting plate 2, a flip motor 45 being installed on the inner wall of the lifting plate 2, the flip motor 45 being located on the left side of the flip plate 44, and the output end of the flip motor 45 being connected to the outer wall of the flip plate 44, a fixed platform 46 being installed on the top of the flip plate 44, and a support platform 3 being sleeved on the outer wall of the fixed platform 46, a micro servo motor 3 47 being installed inside the fixed platform 46, and the micro servo motor 3 The outer wall of the output end of 47 is provided with a rotating frame 48, and a cylindrical rod is installed on the outer wall of the rotating frame 48, an arc frame 49 is installed on the top wall of the support platform 3, and the cylindrical rod is embedded in the inner wall of the arc frame 49, and the cylindrical rod is slidably connected to the inner wall of the arc frame 49, and the inner wall of the box body 1 is slidably connected with a pull-out frame 50, and the pull-out frame 50 is located below the lifting plate 2, and absorbent tissue paper 51 is detachably placed on the inner wall of the pull-out frame 50, and a telescopic rod four 52 is embedded in the bottom wall of the box body 1, and the output end of the telescopic rod four 52 is connected to a push block 53, and the push block 53 is located below the absorbent tissue paper 51.

[0029] Furthermore, after the ELISA plate 5 is washed, the washing liquid is extracted through the washing nozzle, the negative pressure pump and the delivery pipe, and then the flip plate 44 is driven to rotate by the flip motor 45, and the placement frame 4 is rotated to the bottom of the lifting plate 2, and the opening of the ELISA plate 5 is downward, and the micro servo motor 47 drives the rotating frame 48 to rotate, so that the cylindrical rod rotates along the inner wall of the arc frame 49. When the rotating frame 48 drives the cylindrical rod to rotate along the highest point of the inner wall of the arc frame 49 to the lowest point, the rotating rod pushes the support table 3 to move downward along the outer wall of the fixed table 46. When the rotating frame 48 drives the cylindrical rod to rotate along the lowest point of the inner wall of the arc frame 49 to the highest point, the rotating rod pulls the support table 3 to move upward along the outer wall of the fixed table 46, so that the support table 3 drives the placement frame 4 and the ELISA plate 5 to shake up and down, accelerates the falling of the remaining washing liquid in the ELISA plate 5, and facilitates the drying of the ELISA plate 5. Then, the telescopic rod 4 52 is extended to push the push block 53 to move upward, and the absorbent tissue paper 51 is pushed into the ELISA plate 5 to wipe the ELISA plate 5. After that, the telescopic rod 4 52 is shortened to drive the push block 53 to move downward, and then the lifting plate 2 drives the placement frame 4 to move upward to separate the ELISA plate 5 from the absorbent tissue paper 51. The flip motor 45 rotates in the opposite direction, driving the flip plate 44 to rotate in the opposite direction, and rotating the placement frame 4 and the ELISA plate 5 to the top of the lifting plate 2, so as to achieve the purpose of drying the ELISA plate 5 and avoid the presence of washing liquid in the ELISA plate 5 and thus causing adverse effects on the antibody detection results.

[0030] See also Figure 1 and Fig.10An embodiment of the present invention is: a kit for detecting bovine tuberculosis r-interferon antibodies, comprising a pull-out frame 50 including two groups of long rods 54, and the left ends of the two groups of long rods 54 are hinged by a rotating column, a spring 2 55 is installed on the inner wall of the pull-out frame 50, and the spring 2 55 is located between the long rods 54 on both sides, a ball 56 is embedded and installed on the inner wall of the side where the two groups of long rods 54 are close to each other, and the ball 56 is in contact with the absorbent tissue paper 51, and the outer wall of the long rod 54 is provided with a sliding ring sleeve, the sliding ring sleeve is located near the front inner wall of the box body 1, and the sliding ring sleeve is used to fix the two groups of long rods 54.

[0031] The drawer 50 is then pulled outwards and the sliding ring sleeve is slid to the right, so that the inner wall of the sliding ring sleeve is separated from the outer wall of the upper group of long rods 54. Under the push of the spring 2 55, the upper group of long rods 54 are rotated upwards, and then the used absorbent tissue paper 51 is taken out and a new absorbent tissue paper 51 is placed. The size of the absorbent tissue paper 51 is larger than that of the ELISA plate 5. After the absorbent tissue paper 51 is placed, the upper group of long rods 54 are rotated downwards so that the ball bearings 56 limit the absorbent tissue paper 51, and the sliding ring sleeve is pushed backwards so that the sliding ring sleeve is connected to the outer walls of the two groups of long rods 54 at the same time. When the drawer 50 is put back into the box body 1, it is convenient to use the absorbent tissue paper 51 to wipe the ELISA plate 5 again. By setting the ball bearings 56, the smoothness of the absorbent tissue paper 51 moving along the inner wall of the long rod 54 when the push block 53 pushes the absorbent tissue paper 51 upwards is improved.

[0032] See also Figure 1 , Figure 7 and Fig.11An embodiment of the present invention is a kit for detecting bovine tuberculosis r interferon antibody, comprising a pressing frame 58 embedded in the top of a placing frame 4, and the pressing frame 58 is rotatably connected to the top of the placing frame 4, and a fixing pin 60 is installed on the outer wall of the pressing frame 58 near the top of the placing frame 4, and the fixing pin 60 passes through the top of the ELISA plate 5, and a groove is provided on the top of the ELISA plate 5, and the pressing frame 58 is embedded in the inner wall of the groove. When the pressing frame 58 is embedded in the groove, the fixing pin 60 passes through the top of the ELISA plate 5. The ELISA plate 5 is fixed in the hole of the part, and the top of the pressing frame 58 is flush with the top of the ELISA plate 5. The pressing frame 58 is connected to the top of the placement frame 4 by bolts. The outer walls of both sides of the placement frame 4 are provided with friction patterns, and the inner wall of the clamping frame 28 is provided with friction patterns. By providing the friction patterns, the stability of the clamping frame when preventing the frame from being fixed is improved. A support plate is installed on the right side of the partition plate 29. The support plate is located above the waste liquid tank 31 and the washing liquid tank 30. The support plate is used to support the reagents used for bovine tuberculosis r interferon antibody detection. , a bracket is connected to the right side of the partition plate 29, and the bracket is located above the support plate, and a through hole is opened on the top of the bracket. The antibody detection reagent is placed on the inner wall of the through hole, which is convenient for maintaining the stability of the reagent placement and preventing the reagents from colliding with each other or being damaged. Electric heating plates 57 are installed on the inner walls of the front and rear sides of the box body 1, and a battery is embedded and installed at the bottom of the box body 1. A control panel is provided on the front of the box body 1, and the control panel, the electric heating plate 57 and the battery are electrically connected. The electric heating plate 57 is used to incubate the reagents in the ELISA plate 5 inside the box body 1. The inner wall of the partition plate 29 is embedded with a heat insulation layer. By setting the heat insulation layer, the heat generated when the ELISA plate 5 is incubated is prevented from being transferred to the right side of the partition plate 29. Through the arrangement of the electric heating plate 57, the sealing assembly and the washing unit 43, the box body 1 realizes the purpose of sealing, washing, drying and incubating the ELISA plate 5 in an integrated manner, which is conducive to accelerating the efficiency of antibody detection, and avoiding repeatedly taking out and putting in the ELISA plate 5, reducing the possibility of the ELISA plate 5 and the actual being contaminated, which is conducive to improving the accuracy of the antibody detection result.

[0033] Working principle: open the box body 1, take out the reagents and ELISA plate 5 used in the antibody detection process placed in the box body 1, and place the ELISA plate 5 in the placement frame 4 according to the required amount, the lifting plate 2 drives the support platform 3 to move upward until the support platform 3 leaks out of the top of the box body 1, the micro servo motor 25 is started, so that the screw rod 26 drives the moving frame 10 to move backward, leak out of the support platform 3 and install the placement frame 4, add antibody detection standard, diluent, sample, enzyme-labeled conjugate reagent into the ELISA plate 5 in turn, reset the moving frame 10, and then squeeze the roll film 18 to seal the top of the ELISA plate 5 through the pressing plate 23 one and the pressing plate 23 two, move the ELISA plate 5 into the box body 1 for incubation, wash the ELISA plate 5 through the washing unit 43 after incubation, and wipe the ELISA plate 5 dry, move upward again and leak out the ELISA plate 5, add the color developing solution and the stop solution into the ELISA plate 5, take out the ELISA plate 5 after incubation and read the data through the ELISA instrument.

[0034] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A kit for detecting bovine tuberculosis interferon-γ antibodies, characterized in that: It comprises a box body (1) and a film sealing component, wherein the box body (1) is provided with a film sealing component inside, and the film sealing component comprises a lifting plate (2) slidably connected to the inner wall of the box body (1), a telescopic rod (6) and a support platform (3) are installed on the top of the lifting plate (2), and the telescopic rod (6) is located on both sides of the support platform (3), and a placement frame (4) is provided on the top of the support platform (3), and an ELISA plate (5) is detachably installed in the placement frame (4); A horizontal frame (7) is installed on the top of the telescopic rod (6), a micro servo motor (8) is arranged inside the horizontal frame (7), the output end of the micro servo motor (8) is connected to a screw rod (9), the outer wall of the screw rod (9) is provided with a moving frame (10), and the moving frame (10) is located above the ELISA plate (5), the top wall of the moving frame (10) is connected to a placement frame, the back of the placement frame is provided with a winding motor (12), the output end of the winding motor (12) is connected to the winding frame (13), and the moving frame (10) A linear motor unit (14) is arranged on the top wall of the movable frame (10), the linear motor unit (14) comprises a linear guide rail mounted on the top wall of the movable frame (10), the outer wall of the linear guide rail is slidably connected to the linear motor, the output end of the linear motor is connected to the mounting platform, an adjustment frame (15) is installed at the bottom of the mounting platform, and the adjustment frame (15) is located on the right side of the movable frame (10), an unwinding motor (16) is arranged on the back side of the adjustment frame (15), the output end of the unwinding motor (16) is connected to the unwinding frame (17), and the outer wall of the unwinding frame (17) is provided with a roll film (18); The bottom wall of the movable frame (10) is connected to a pressing frame 1 (19), the outer wall of the pressing frame 1 (19) is slidably connected to a pressing frame 2 (20), and the pressing frame 1 (19) and the pressing frame 2 (20) are located between the fixed frame (11) and the adjustment frame (15), the film roll (18) is located between the pressing frame 1 (19) and the ELISA plate (5), the top wall of the pressing frame 1 (19) is installed with a spring 1 (22), the bottom end of the spring 1 (22) is connected to a pressing plate (23), and the pressing plate (23) is embedded and connected to the inner wall of the pressing frame 1 (19), the inner wall of the pressing frame 1 (19) is embedded and installed with an electromagnet (24), and the electromagnet (24) is located below the pressing plate (23), and the top of the pressing frame 2 (20) is installed with a connecting rod (21), and the connecting rod (21) is connected to the outer wall of the adjustment frame (15).

2. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 1, characterized in that: A second telescopic rod (27) is embedded and installed on the inner wall of the support platform (3); the output end of the second telescopic rod (27) is connected to a clamping frame (28), and the clamping frame (28) is used to fix the placement frame (4).

3. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 1, characterized in that: The inner wall of the box body (1) is installed with a partition plate (29), the lifting plate (2) is located on the left side of the partition plate (29), a waste liquid tank (31) is arranged on the right side of the partition plate (29), and a washing liquid tank (30) is arranged on the right side of the waste liquid tank (31). The left outer wall of the partition plate (29) is provided with a mounting groove (32), and a rack (33) is embedded and installed in the inner wall of the mounting groove (32). A rack (34) is installed on the top of the lifting plate (2), and the rack (34) is located on the front and rear sides of the support platform (3), and the rack (34) and the rack (33) are located on the same straight line. The outer wall of the rack (33) is slidably connected with a sliding frame (35), and a rack (36) is installed on the top of the sliding frame (35). A driving motor (37) is installed on the inner wall of the sliding frame (35), and a gear (38) is sleeved on the outer wall of the output end of the driving motor (37), and the gear (38) is connected to the outer wall of the output end of the driving motor (37). The rack one (33), the rack two (34) and the rack three (36) are meshed, a vertical rod (39) is installed on the top of the rack three (36), a blocking block (40) is installed on the top of the vertical rod (39), and the blocking block (40) passes through the outer wall of the partition plate (29), a horizontal plate (41) is installed on the right side of the partition plate (29), a telescopic rod three (42) is installed on the bottom of the horizontal plate (41), the output end of the telescopic rod three (42) is connected to the washing unit (43), the washing unit (43) comprises an L-shaped bracket connected to the output end of the telescopic rod three (42), the top of the L-shaped bracket is connected to the washing nozzle, a negative pressure pump and a delivery pump are arranged on the top of the waste liquid tank (31), and the input end and output end of the delivery pump and the negative pressure pump are both provided with delivery pipes, the washing nozzle is connected to the washing liquid tank (30) through the delivery pipe and the delivery pump, and the washing nozzle is connected to the waste liquid tank (31) through the delivery pipe and the negative pressure pump.

4. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 3, characterized in that: The bottom wall of the box body (1) is mounted with a second micro servo motor (25), the output end of the second micro servo motor (25) is connected with a second screw rod (26), and the lifting plate (2) is sleeved on the outer wall of the second screw rod (26), and the second screw rod (26) is located at the front and rear sides of the second rack (34).

5. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 4, characterized in that: A flip plate (44) is provided through the top of the lifting plate (2), a flip motor (45) is installed on the inner wall of the lifting plate (2), and the output end of the flip motor (45) is connected to the outer wall of the flip plate (44), a fixed platform (46) is installed on the top of the flip plate (44), and the support platform (3) is sleeved on the outer wall of the fixed platform (46), a micro servo motor (47) is installed inside the fixed platform (46), and a rotating frame (48) is sleeved on the outer wall of the output end of the micro servo motor (47), and the outer wall of the rotating frame (48) is A cylindrical rod is installed, an arc frame (49) is installed on the top wall of the support platform (3), and the cylindrical rod is embedded and connected to the inner wall of the arc frame (49), the inner wall of the box body (1) is slidably connected to a pull-out frame (50), and the pull-out frame (50) is located below the lifting plate (2), and absorbent tissue paper (51) is detachably placed on the inner wall of the pull-out frame (50), and a telescopic rod four (52) is embedded and installed on the bottom wall of the box body (1), and the output end of the telescopic rod four (52) is connected to a push block (53), and the push block (53) is located below the absorbent tissue paper (51).

6. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 5, characterized in that: The pull-out frame (50) comprises two groups of long rods (54), and the left ends of the two groups of long rods (54) are hinged via a rotating column. A second spring (55) is installed on the inner wall of the pull-out frame (50), and the second spring (55) is located between the long rods (54) on both sides. A ball (56) is embedded and installed on the inner wall of the two groups of long rods (54) on one side close to each other, and the ball (56) is in contact with the absorbent tissue paper (51).

7. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 5, characterized in that: Electric heating plates (57) are installed on the inner walls of the front and rear sides of the box body (1).

8. A kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 1, characterized in that: A pressing frame (58) is embedded and connected to the top of the placement frame (4), and a fixing pin (60) is installed on the outer wall of one side of the pressing frame (58) close to the top of the placement frame (4), and the fixing pin (60) passes through the top of the ELISA plate (5).

9. A method for detecting bovine tuberculosis interferon-γ antibodies, applicable to the kit for detecting bovine tuberculosis interferon-γ antibodies according to claim 7, characterized in that: The detection method of bovine tuberculosis interferon-γ antibody is as follows: S1, opening the box body (1), taking out the reagents and ELISA plate (5) used in the antibody detection process placed in the box body (1), and placing the ELISA plate (5) in the placement frame (4) according to the required amount; S2, the lifting plate (2) drives the support platform (3) to move upward until the support platform (3) is exposed to the top of the box body (1), and the second micro servo motor (25) is started, so that the second screw rod (26) drives the moving frame (10) to move backward, the support platform (3) is exposed, and the placement frame (4) is installed; S3, adding antibody detection standard, diluent, sample, and enzyme-labeled conjugate reagent into the ELISA plate (5) in sequence, resetting the movable frame (10), and then sealing the top of the ELISA plate (5) by squeezing the roll film (18) through the pressing plate (23) 1 and the pressing plate (23) 2; S4, moving the ELISA plate (5) into the box body (1) for incubation, washing the ELISA plate (5) through the washing unit (43) after incubation, and wiping the ELISA plate (5) dry; S5, perform S1 again, add the color developing solution and the stop solution into the ELISA plate (5), take out the ELISA plate (5) after incubation, and read the data using an ELISA reader.

10. The method for detecting bovine tuberculosis interferon-γ antibodies according to claim 9, characterized in that: In S3, the following steps are also included: S31, adjusting the positions of the second pressing frame (20) and the adjusting frame (15) according to the number of groups of the ELISA plates (5) used, and controlling the pulling length of the rolled film (18); In S4, the following steps are also included: S41. When drying the ELISA plate (5), firstly, the support platform (3) is turned over by the turning plate (44), the ELISA plate (5) is rotated to the position where the opening faces downward, and then the absorbent tissue paper (51) is pushed into the ELISA plate (5) by the pushing block (53) to absorb the residual moisture.

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