Agricultural combination

Abstract

The present disclosure relates to an agricultural combination comprising at least one systemic fungicide and a plant health promoting additive and compositions comprising the same. The combination has a synergistic effect and is suitable for controlling phytopathogenic fungi. Thus, the present disclosure relates to an agricultural combination comprising tricyclazole and orthosilicic acid. The present disclosure also relates to a method of reducing the application, residue or residue of tricyclazole in field mud and agricultural products.

Description

Technical Field

[0001] The present disclosure relates to an agricultural combination comprising at least one systemic fungicide and a plant health promoting additive and a composition comprising the combination. The present disclosure also relates to a method for reducing systemic fungicide residues or residuals in field mud and agricultural products. The combination has a synergistic effect and is suitable for controlling plant pathogenic fungi. Background Art

[0002] Tricyclazole (8-methyl-[1,2,4]triazolo[3,4-b][1,3]benzothiazole) is a systemic fungicide used worldwide to treat seasonal rice blast disease in rice crops. Tricyclazole protects the plant from infection by phytopathogenic fungi by preventing fungal penetration into the epidermis. The compound works by inhibiting melanin formation within appressorium, resulting in a lack of rigidity in the appressorium wall. The tricyclazole belongs to the benzothiazole class of chemicals and is recommended for the control of Magnaporthe oryzae, commonly known as rice blast, one of the most important diseases of rice crops. The tricyclazole fungicide has a preventive effect and should be applied to rice crops before panicles, spikelets and tillers are infected.

[0003] However, there is limited information on the migration and fate of tricyclazole in rice fields. Some studies have detected tricyclazole residues in water samples after rice harvest and pointed out that variables such as season and year are important factors in determining the distribution of tricyclazole residues. The researchers also emphasized that because tricyclazole is not easily hydrolyzed in the environment, it is highly persistent in water-soil systems, with a half-life of 4 to 17 months under laboratory conditions and 6 months under field conditions.

[0004] The study further showed that tricyclazole is moderately toxic and causes increased genotoxic activity in water of rice crops through changes in chromosomes in onion roots. Tricyclazole fungicides can also disrupt the functioning of different body organs like the liver by inhibiting cytochrome CYP450 and cholesterol synthesis. While monitoring chemical contaminants in food, it was found that pesticides like tricyclazole were often found even in processed white rice, parboiled rice and cooked rice. The European Union (EU) imports 10% of the rice exported by India. In January 2018, the EU set and implemented new standards for the maximum residue level (MRL) of tricyclazole used by most of the basmati rice farmers in the country. The MRL for basmati rice was reduced from 1 ppm to 0.01 ppm. The EU's move has hit the rice export and trade market in India, and the selling price of rice in the European market has also risen rapidly.

[0005] Small amounts of pesticide residues in field slurries or produce and repeated exposure over time can have chronic effects. Therefore, choosing alternatives in agricultural practices to reduce the accumulation of pesticide residues and their potential harmful effects is critical to safeguarding human well-being, soil microbiota, and environmental health.

[0006] There is a need to reduce the application of high doses of tricyclazole in crop plantations by combining tricyclazole with a plant health promoting additive, wherein the combination enhances the fungicidal activity of tricyclazole by the plant health promoting additive, has a synergistic effect and reduces the residues of tricyclazole.

[0007] Purpose of the Invention

[0008] An object of the present disclosure is to provide a combination / composition comprising a systemic fungicide and a plant growth promoting additive.

[0009] Another object of the present disclosure is to provide a combination / composition comprising a systemic fungicide and a plant growth promoting additive to achieve the following advantages;

[0010] Reduce the application rate of systemic fungicides;

[0011] Significantly reduce residual levels of systemic fungicides in crop products and field mud;

[0012] synergistically inhibit fungal growth; and

[0013] Improve crop yield and agricultural product quality.

[0014] Another object of the present disclosure is to provide a method for reducing fungicide residues or residuals in field slurries and agricultural products.

[0015] Another object of the present disclosure is to provide a method for improving crop yield, health, and nutrition of agricultural products. Summary of the invention

[0016] The present disclosure relates to an agricultural combination comprising at least one systemic fungicide and a plant health promoting additive.

[0017] In one aspect, the present disclosure is directed to an agricultural combination comprising at least one systemic fungicide comprising at least one group of melanin synthesis inhibitor fungicides and a plant health promoting additive.

[0018] In another aspect, the present disclosure is directed to an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive.

[0019] In another aspect, the present disclosure relates to an agricultural combination comprising tricyclazole and orthosilicic acid.

[0020] In one aspect, the present disclosure is directed to an agricultural composition comprising at least one systemic fungicide comprising at least one group of melanin synthesis inhibitor fungicides, a plant health promoting additive, and an agriculturally acceptable excipient.

[0021] In one aspect, the present disclosure relates to an agricultural composition comprising at least one systemic fungicide comprising at least one group of melanin synthesis inhibitor fungicides, a silicic acid-based plant health promoting additive, and an agriculturally acceptable excipient.

[0022] In one aspect, the present disclosure relates to an agricultural composition comprising tricyclazole, orthosilicic acid, and an agriculturally acceptable excipient.

[0023] In another aspect, the present disclosure relates to the use of an agricultural combination comprising a systemic fungicide and a silicate-based plant health promoting additive for reducing the application dosage of the systemic fungicide in crop plantations; synergistically inhibiting fungal growth; significantly reducing the residual level of the systemic fungicide in rice plantations and field mud; and improving crop yield, nutrition and agricultural product health.

[0024] In another aspect, the present disclosure relates to the use of an agricultural combination comprising tricyclazole and a silicate-based plant health promoting additive for reducing the application dosage of tricyclazole in crop plantations; synergistically inhibiting fungal growth; significantly reducing the residual level of tricyclazole in rice plantations and field mud; and improving crop yield, nutrition and produce health.

[0025] In another aspect, the present disclosure is directed to a method of reducing systemic fungicide residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising a systemic fungicide and a silicic acid-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of the systemic fungicide.

[0026] In another aspect, the present disclosure relates to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of tricyclazole.

[0027] In another aspect, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to a locus, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of the tricyclazole.

[0028] In another aspect, the present disclosure provides a method for increasing crop yield, the method comprising applying the agricultural combination of the present disclosure to a crop, plant propagation material, or agricultural product surface.

[0029] In another aspect, the present disclosure provides a kit. The kit includes a plurality of components, each of which may include at least one or more ingredients of the fungicide combination / composition of the present disclosure. DETAILED DESCRIPTION

[0030] The following description is provided to assist in a comprehensive understanding of the exemplary embodiments of the present disclosure. It includes various specific details to assist in understanding, but these are considered to be exemplary only. Therefore, those of ordinary skill in the art will recognize that various changes and modifications may be made to the embodiments described herein without departing from the scope of the present disclosure. In addition, for clarity and conciseness, descriptions of well-known functions and structures are omitted.

[0031] The terms used in the following description and claims are not limited to the bibliographical meanings, but are used only by the inventors to enable a clear and consistent understanding of the present disclosure. Therefore, it should be clear to those skilled in the art that the following description of exemplary embodiments of the present disclosure is provided for illustration only and is not intended to limit the scope of the present disclosure as defined by the claims and their equivalents.

[0032] For the purpose of this disclosure, it should be understood that the disclosure can take various alternative variations and step sequences, unless there is a clear contrary provision. In addition, except in any operating examples or otherwise indicated, all numbers representing the amount of materials / ingredients used in the specification should be understood to be modified by the term "about" in all cases.

[0033] Therefore, before describing the present disclosure in detail, it should be understood that the present disclosure is not limited to the systems or process parameters specifically exemplified, which may of course vary. It should also be understood that the terms used herein are only used to describe embodiments of the present disclosure and are not intended to limit the scope of the present disclosure in any way. The use of examples anywhere in this specification (including examples of any term discussed herein) is illustrative only and in no way limits the scope and meaning of the present disclosure or any exemplified term.

[0034] Before elaborating on the present subject matter in detail, it may be helpful to provide definitions of certain terms used herein. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present subject matter belongs. For the sake of clarity, the following definitions are provided.

[0035] The use of the terms "a", "an", "the" and similar referents (especially in the context of the claims) should be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context. The terms first, second, etc., as used herein, are not meant to indicate any order, but are merely for convenience to indicate a plurality, such as layers.

[0036] The terms "comprising," "having," "including," and "containing" should be construed as open-ended terms (ie, meaning "including, but not limited to,") unless otherwise noted.

[0037] In any aspect or embodiment described below, the phrase "comprising" may be replaced by the phrase "consisting of" or "consisting essentially of. In these aspects or embodiments, the combination or composition comprises or includes or consists essentially of or consists essentially of the specific components recited therein, excluding other ingredients or excipients not specifically recited therein.

[0038] As used herein, "about" or "approximately" includes the stated value and the average value within the acceptable deviation range of the specific value determined by a person of ordinary skill in the art, taking into account the measurement in question and the errors associated with the measurement of the specific amount (i.e., the limitations of the measurement system). For example, "about" can mean within one or more standard deviations, or within ±10% or ±5% of the value. Unless otherwise specified herein, the description of the numerical range is intended only to be used as a shorthand method for individually referring to each individual value falling within the range, and each individual value is incorporated into the specification as if it were individually described herein. The endpoints of all ranges are included in the range and can be independently combined. It should be understood that where a parameter range is provided, all integers and tenths thereof within the range are also provided. For example, "0.1-80%" includes 0.1%, 0.2%, 0.3%, etc. up to 80%.

[0039] All methods described herein can be performed in a suitable order unless otherwise stated herein or clearly contradicted by context. Unless otherwise stated, the use of any and all examples or exemplary language (e.g., "such as") is intended only to better illustrate the present disclosure and does not limit the scope of the present disclosure. No language in the specification should be construed to indicate that any unclaimed element is essential to the practice of the present disclosure as used herein.

[0040] As used herein, the term "agricultural" is understood to mean agricultural chemicals, such as pesticides, fungicides, insecticides, miticides, herbicides, nematicides, plant growth regulators, and may be used interchangeably.

[0041] The term "active" as used herein refers to a component that is chemically and / or biologically active. The term "active ingredient" as used herein refers to tricyclazole or its salts, esters, or derivatives. Similarly, the term active ingredient refers to orthosilicic acid or its derivatives. In this regard, the "active" ingredient can be a single ingredient or a combination of multiple ingredients; and the meaning of the term "active" should be understood to include, but is not limited to, "benzamide fungicides" or benzamidine or its salts or esters.

[0042] The phrase "fungicidally effective amount of a systemic fungicide" refers to an amount of a systemic fungicide that kills or inhibits the plant pathogenic fungi or diseases to be controlled, but which is not significantly toxic to the plants being treated.

[0043] The phrase "fungicidally effective amount of tricyclazole" refers to an amount of tricyclazole that kills or inhibits the plant pathogenic fungi or diseases to be controlled, which is not significantly toxic to the treated plants. This means that a standard full dosage rate of tricyclazole in the range of 300 to 600 g / Ha can effectively control plant pathogenic fungi.

[0044] Control of Rice Blast with Tricyclazole 75% WP

[0045] Bioefficacy Evaluation and Residue Analysis by Sekhar Bandyopadhyay, Parthendu Poddar and Goutam Kumar Pandit.

[0046] Control of Rice Blast with Tricyclazole 75% WP

[0047] Bioefficacy Evaluation and Residue Analysis by Sekhar Bandyopadhyay, Parthendu Poddar and Goutam Kumar Pandit.

[0048] Control of Rice Blast with Tricyclazole 75% WP

[0049] Bioefficacy Evaluation and Residue Analysis by Sekhar Bandyopadhyay, Parthendu Poddar and Goutam Kumar Pandit.

[0050] Tricyclazole 75% WP for controlling rice blast in rice International Journal of Latest Scientific Research Vol. 6, No. 11,

[0051] Pages 7254-7258, November 2015

[0052] Bioefficacy Evaluation and Residue Analysis by Sekhar Bandyopadhyay, Parthendu Poddar and Goutam Kumar Pandit.

[0053] Tricyclazole 75% WP for controlling rice blast in rice International Journal of Latest Scientific Research Vol. 6, No. 11,

[0054] Pages 7254-7258, November 2015

[0055] As used herein, the term "disease control" means controlling and preventing diseases. Control effects include all deviations from natural development, for example: killing, retarding, inhibiting or reducing fungal diseases.

[0056] The term "plant" or "crop" refers to a whole plant, a plant organ (e.g., leaves, stems, shoots, roots, trunks, branches, twigs, fruits, etc.), a plant cell or a plant seed. This term also includes plant crops, such as fruits. The term "plant" may also include ''propagation material thereof'', which may include all reproductive parts of the plant, such as seeds and vegetative plant material, such as cuttings and tubers, which may be used for the propagation of the plant. This includes seeds, tubers, spores, corms, bulbs, rhizomes, buds, basal buds, runners and shoots and other parts of the plant, including seedlings and seedlings transplanted after germination or after emergence from the soil.

[0057] The term "plant propagation material" is understood to refer to the reproductive parts of plants, such as seeds, nutrient materials (such as cuttings or tubers), roots, fruits, tubers, corms, rhizomes, and other parts of plants, germinating plants and / or seedlings, which will be transplanted after germination or after emerging from the soil. These seedlings can be protected by all or part of the immersion process before transplanting.

[0058] The term "site" as used herein means a vicinity or area designated for growing a desired crop susceptible or potentially susceptible to insect attack, and where control of plant pathogenic fungi is desired. The site includes the vicinity of the desired crop plants where fungal attack has occurred, is most likely to occur, or has not yet occurred.

[0059] The term "agricultural product" means any product produced by the growing of plants and the raising of animals of all kinds for use as food, fiber, fuel, raw material or other similar product, which is either not further processed or which is subjected to a process usually performed by the grower or producer which does not change its essential characteristics but renders it marketable in the principal market.

[0060] The term "% disease severity" refers to the percentage of decay observed in a crop as a percentage of the associated host tissue covered by lesions or damaged by the disease in the crop. The number and size of lesions contribute to the severity. The % severity indicates the extent of damage caused by the disease.

[0061] The term "fungicide" refers to the ability of a substance to reduce or inhibit the growth of fungi or oomycetes.

[0062] "Control" or "controlling" fungi means inhibiting and / or suppressing the ability of fungi to grow and / or reproduce, or limiting fungus-related damage or loss in crop plants, or means disease control and prevention. Control effects include all deviations from natural development, e.g., killing, delaying, reducing fungal diseases.

[0063] As used herein, the term % disease control refers to the % control and prevention of diseases in crops.

[0064] As used herein, Magnaporthe grisea, also known as Magnaporthe oryzae, Pyricularia grisea, Pyricularia oryzae, Rice Neck Rot, Rice Seedling Blight, Rice Blast, Grain Oval Leaf Spot, Pitting, Ryegrass Blast, Neck Blast, Wheat Blast and Imochi, are synonyms of each other and refer to the same plant pathogen.

[0065] As used herein, % disease control refers to the % control of leaf blast and the plant pathogen causing the disease, Pyricularia spp. or Pyricularia oryzae, achieved in rice crops.

[0066] According to the present disclosure, "yield improvement" of an agricultural plant means that the yield of the corresponding plant product is increased by a measurable amount compared to the yield of the same plant product produced under the same conditions without the application of the present disclosure.

[0067] As used herein, the term "gai / L" or "g ai / L" means the concentration of the corresponding active ingredient in "grams" present in "per liter" of the composition.

[0068] The term "gai / ha" or "gai / ha" as used herein means the concentration of the corresponding active ingredient in "grams" applied "per hectare" of crop field.

[0069] Reference to ai or ai refers to the active ingredient and is interpreted as having the same meaning in the following parts of this specification.

[0070] Reference to plant health promoting additives / promoters is synonymous.

[0071] Each of the above aspects may have one or more embodiments.

[0072] Each embodiment described below is applicable to one or all aspects described above. These embodiments are intended to be interpreted as preferred features of one or all aspects described above. Each embodiment described below is applicable to each aspect described above individually.

[0073] In view of the above, it can be seen that several advantages of the present disclosure have been achieved, and the present disclosure has also achieved other favorable results. Although the present disclosure has been fully disclosed, it should be understood that many additional modifications and changes can be made thereto without departing from the scope of the present disclosure. The embodiments can be combined together to better understand the present disclosure without departing from the scope of the present disclosure.

[0074] In another embodiment, the alternative or multiple embodiments disclosed herein should not be understood as limiting. Each embodiment may be cited and claimed individually or in any combination with other embodiments of the present disclosure. Without departing from the scope of the present disclosure, one or more embodiments of the present disclosure may be combined to show the teachings of the present disclosure.

[0075] Through the present disclosure, the inventors have successfully attempted to alleviate the problems associated with residues of systemic fungicides (i.e., tricyclazoles found in farm mud, soil, and even in agricultural produce from crops that farmers treat with such systemic fungicides). Excessive pesticide residues negatively impact the export prospects of crop products, ultimately undermining the economic benefits that could otherwise be generated, given the restrictions imposed by regulatory agencies.

[0076] The inventors unexpectedly discovered that the application of an agricultural combination comprising a systemic fungicide (i.e., tricyclazole) and a plant growth promoting additive (i.e., a silica-based plant growth promoter / additive) significantly reduced the residual levels of tricyclazole in crop plantations and field mud, and improved crop yield, nutrition, and agricultural product health. The inventors reduced the application amount of the systemic fungicide (i.e., tricyclazole) in the agricultural combination by about 60% of the standard dosage used in agricultural applications, and added a silica-based plant growth promoting additive to the combination. In addition, the inventors also noted that the reduction in the application amount of tricyclazole did not impair the antifungal activity of the agricultural combination, and in fact, the synergistic activity of the combination became apparent in controlling the growth and spread of plant pathogenic fungi.

[0077] Rice blast (Magnaporthe oryzae) is a key concern for achieving global food security as the disease causes approximately 30% of global rice production losses, equivalent to the food production of 60 million people. These losses can increase global rice prices and reduce consumer welfare and food security. Yield losses from rice blast infection depend on variety susceptibility, infection level, and timing of fungicide applications. Some yield losses associated with rice blast outbreaks have reached 50% or more, and the costs of these rice blast outbreaks are mitigated by fungicide applications. Rice blast outbreaks sometimes occur under abnormal weather conditions such as low temperatures or sudden rainfall in summer, so rice blast is one of the most serious rice diseases worldwide, and new strategies for rice blast control are urgently needed.

[0078] Furthermore, the growing global demand for rice, coupled with unpredictable weather events over the past few years, has put tremendous pressure on rice farmers in the region. This has forced farmers to find new ways to grow higher quality rice in an efficient and sustainable manner. In addition, restrictions on the Maximum Residue Level (MRL) for basmati rice have further adversely affected rice production.

[0079] The present invention thus provides an efficient and environmentally safe agricultural combination that successfully overcomes imposed regulatory constraints and contributes to social welfare and global food security.

[0080] The present disclosure provides an agricultural combination comprising at least one systemic fungicide and a plant health promoting additive / promoter.

[0081] In one embodiment, the plant health promoting additive comprises a silicate-based additive.

[0082] In one embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silica-based plant additive.

[0083] In one embodiment, the systemic fungicide of the present disclosure is selected from the group consisting of demethylation inhibitor fungicides, nucleic acid synthesis inhibitors, cytoskeleton and motor protein inhibitors, amino acid and protein synthesis inhibitors, respiratory process inhibitors, signal transduction inhibitors, lipid synthesis and membrane integrity disruptors, sterol biosynthesis inhibitors, melanin synthesis inhibitors, cell wall biosynthesis inhibitors, host plant defense inducers and / or fungicides with unknown modes of action.

[0084] In a preferred embodiment, the systemic fungicide comprises a melanin synthesis inhibitor or a mixture thereof.

[0085] In one embodiment, the present disclosure provides an agricultural combination, the agricultural combination comprising:

[0086] melanin synthesis inhibitor fungicides, including isobenzofuranones including fthalide; pyrroloquinolinones including pyroquilon; triazolothiazoles including tricyclazole; cyclopropanecarboxamides including carpropamid; carboxamides including diclocymet; propionamides including fenoxanil; trifluoroethylcarbamates including tolprocarb and mixtures thereof; and

[0087] At least one silicate-based additive.

[0088] In one embodiment, the melanin synthesis inhibitor fungicide comprises isobenzofuranones (such as fthalide), pyrroloquinolinones (such as pyroquilon), triazolothiazoles (such as tricyclazole), cyclopropanecarboxamides (such as carpropamid), carboxamides (such as diclocymet), propionamides (such as fenoxanil), trifluoroethylcarbamates (such as tolprocarb), and mixtures thereof.

[0089] In a preferred embodiment, the melanin synthesis inhibitor fungicide comprises tricyclazole or a salt or derivative thereof.

[0090] In one embodiment, the systemic fungicide comprises a demethylation inhibitor fungicide.

[0091] In one embodiment, the demethylation inhibitor fungicide comprises a triazole fungicide.

[0092] In one embodiment, the triazole fungicide is selected from the group consisting of oxepiconazole, oxadiazole, cyproconazole, benzyltriazole, difenoconazole, diniconazole, R-diniconazole, fluconazole, eticonazole, fenbuconazole, fluconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furoconazole, hexaconazole, imipenem, picroconazole, isotrifluconazole, clofoconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, quinconazole, silyfloxacin, tebuconazole, tetraconazole, thiabendazole, triadimefon, triadimenol, triathione, uniconazole and uniconazole, their salts or esters, or isomers or derivatives, or combinations thereof.

[0093] In one embodiment, the plant health promoting additive according to the present disclosure is selected from a silica-based plant growth promoting additive having the following general formula:

[0094] [SiO x (OH) 4-2x ] n

[0095] In one embodiment, n ranges from 6 to 200.

[0096] In one embodiment, x is 1.5 and n is 20.

[0097] In one embodiment, x is 1.5 and n is 8, or 6, or 10, or greater than 16.

[0098] In one embodiment, x is 1.5 and n is greater than 20.

[0099] In one embodiment, x is 1.5 and n is 36.

[0100] In one embodiment, the silicate-based additive is selected from the group consisting of metasilicic acid (H2SiO3), orthosilicic acid (H4SiO4), disilicate (H2Si2O5) and pyrosilicate (H6Si2O7); orthosilicic acid is preferred.

[0101] In a preferred embodiment, the plant growth promoting additive comprises orthosilicic acid (H4SiO4).

[0102] In another preferred embodiment, the plant growth promoting additive comprises stabilized orthosilicic acid (H4SiO4).

[0103] In one embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive.

[0104] In one embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and stabilized orthosilicic acid.

[0105] In one embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced from about 0.1% to about 99% of the fungicidally effective amount of the systemic fungicide.

[0106] In one embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced from about 1% to about 80% of the fungicidally effective amount of the systemic fungicide.

[0107] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced from about 1% to about 60% of the fungicidally effective amount of the systemic fungicide.

[0108] In another preferred embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced from about 1% to about 55% of the fungicidally effective amount of the systemic fungicide.

[0109] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced by about 1% to about 50% of the fungicidally effective amount of the systemic fungicide.

[0110] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced by about 1% to about 40% of the fungicidally effective amount of the systemic fungicide.

[0111] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of the systemic fungicide in the combination is reduced by about 1% to about 30% of the fungicidally effective amount of the systemic fungicide.

[0112] In one embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 0.1% to about 99% of the fungicidally effective amount of the fungicide.

[0113] In one embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 80% of the fungicidally effective amount of the fungicide.

[0114] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of the fungicide.

[0115] In another preferred embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 55% of the fungicidally effective amount of the fungicide.

[0116] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 50% of the fungicidally effective amount of the fungicide.

[0117] In a preferred embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 40% of the fungicidally effective amount of the fungicide.

[0118] In another preferred embodiment, the present disclosure provides an agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 30% of the fungicidally effective amount of the fungicide.

[0119] In one embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced from about 0.1% to about 99% of the fungicidally effective amount of tricyclazole.

[0120] In one embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 80% of the fungicidally effective amount of tricyclazole.

[0121] In a preferred embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole.

[0122] In a preferred embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 55% of the fungicidally effective amount of tricyclazole.

[0123] In a preferred embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50% of the fungicidally effective amount of tricyclazole.

[0124] In a preferred embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 40% of the fungicidally effective amount of tricyclazole.

[0125] In a preferred embodiment, the present disclosure provides an agricultural combination comprising tricyclazole and orthosilicic acid as a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 30% of the fungicidally effective amount of tricyclazole.

[0126] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 60% of the fungicidally effective amount of tricyclazole.

[0127] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 55% of the fungicidally effective amount of tricyclazole.

[0128] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 50% of the fungicidally effective amount of tricyclazole.

[0129] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 40% of the fungicidally effective amount of tricyclazole.

[0130] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 37.5% of the fungicidally effective amount of tricyclazole.

[0131] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 30% of the fungicidally effective amount of tricyclazole.

[0132] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 25% of the fungicidally effective amount of tricyclazole.

[0133] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 15% of the fungicidally effective amount of tricyclazole.

[0134] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 12.5% ​​of the fungicidally effective amount of tricyclazole.

[0135] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 10% of the fungicidally effective amount of tricyclazole.

[0136] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 6.25% of the fungicidally effective amount of tricyclazole.

[0137] In one embodiment, the amount of tricyclazole in the agricultural combination is reduced by about 5% of the fungicidally effective amount of tricyclazole.

[0138] In a preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 10 g ai / ha to 300 g ai / ha.

[0139] In a preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 50 g ai / ha to 300 g ai / ha.

[0140] In a preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 300 g ai / ha.

[0141] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 299 g ai / ha.

[0142] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 290 g ai / ha.

[0143] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 285 g ai / ha.

[0144] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 270 g ai / ha.

[0145] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 300 g ai / ha.

[0146] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 299 g ai / ha.

[0147] In another preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 290 g ai / ha.

[0148] In a more preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 285 g ai / ha.

[0149] In a more preferred embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 270 g ai / ha.

[0150] In one embodiment the agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 300 g ai / ha.

[0151] In one embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 299 g ai / ha.

[0152] In one embodiment, the agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 290 g ai / ha.

[0153] In one embodiment, the agricultural combination comprises 150 g ai / ha of tricyclazole.

[0154] In one embodiment, the agricultural combination comprises 187 g ai / ha of tricyclazole.

[0155] In one embodiment, the agricultural combination comprises 225 g ai / ha of tricyclazole.

[0156] In one embodiment, the agricultural combination comprises 244 g ai / ha of tricyclazole.

[0157] In one embodiment, the agricultural combination comprises 262 g ai / ha of tricyclazole.

[0158] In one embodiment, the agricultural combination comprises 281 g ai / ha of tricyclazole.

[0159] In one embodiment, the agricultural combination comprises orthosilicic acid in an amount ranging from 1 g ai / ha to 100 g ai / ha.

[0160] In one embodiment, the agricultural combination comprises orthosilicic acid in an amount ranging from 1 g ai / ha to 70 g ai / ha.

[0161] In a preferred embodiment the agricultural combination comprises orthosilicic acid in an amount ranging from 10 g ai / ha to 50 g ai / ha.

[0162] In a preferred embodiment the agricultural combination comprises orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0163] In a preferred embodiment the agricultural combination comprises orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0164] In a preferred embodiment, the agricultural combination comprises 30 g ai / ha of orthosilicic acid.

[0165] In a preferred embodiment, the agricultural combination comprises 25 g ai / ha of orthosilicic acid.

[0166] In a preferred embodiment, the agricultural combination comprises 20 g ai / ha of orthosilicic acid.

[0167] In one embodiment, the agricultural combination comprising tricyclazole and orthosilicic acid is applied as a tank mix.

[0168] In one embodiment, the application rate of tricyclazole in the tank mix is ​​50 g / ha to 600 g / ha.

[0169] In a preferred embodiment, the application rate of tricyclazole in the tank mix is ​​50 g / ha to 400 g / ha.

[0170] In a preferred embodiment, the application rate of tricyclazole in the tank mix is ​​100 g / ha to 400 g / ha.

[0171] In a preferred embodiment, the application rate of tricyclazole in the tank mix is ​​200 g / ha to 380 g / ha.

[0172] In one embodiment, the application rate of orthosilicic acid in the tank mix is ​​250 ml / ha to 2500 ml / ha.

[0173] In a preferred embodiment, the application rate of orthosilicic acid in the tank mix is ​​500 ml / ha to 2000 ml / ha.

[0174] In a preferred embodiment, the application rate of orthosilicic acid in the tank mix is ​​1000 ml / ha to 2000 ml / ha.

[0175] In another preferred embodiment, the application rate of orthosilicic acid in the tank mix is ​​1000 ml / ha to 1800 ml / ha.

[0176] In a preferred embodiment, the application rate of orthosilicic acid in the tank mix is ​​1250 ml / ha.

[0177] In a preferred embodiment, the application rate of orthosilicic acid in the tank mix is ​​1500 ml / ha.

[0178] In one embodiment, the components of the combination of the present invention can be mixed in the ratios of (1-80):(1-80), (1-70):(1-70), (1-60):(1-60), (1-50):(1-50), (1-40):(1-40), (1-30):(1-30), (1-20):(1-20), (1-10):(1-10), and (1-5):(1-5) of the systemic fungicide and the silicate-based plant health promoting additive, respectively.

[0179] In one embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:30 to 30:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0180] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:20 to 20:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0181] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:15 to 15:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0182] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:10 to 10:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0183] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 20:1 to 4:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0184] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 15:1 to 4:1 of the systemic fungicide and the silica-based plant health promoting additive, respectively.

[0185] In one embodiment, the components of the combination of the present invention can be mixed according to the ratios of (1-80):(1-80), (1-70):(1-70), (1-60):(1-60), (1-50):(1-50), (1-40):(1-40), (1-30):(1-30), (1-20):(1-20), (1-10):(1-10), and (1-5):(1-5) of melanin synthesis inhibitor fungicide and silicate-based plant health promoting additive, respectively.

[0186] In one embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:30 to 30:1 of the melanin synthesis inhibitor fungicide and the silica-based plant health promoting additive, respectively.

[0187] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:20 to 20:1 of the melanin synthesis inhibitor fungicide to the silica-based plant health promoting additive, respectively.

[0188] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:15 to 15:1 of the melanin synthesis inhibitor fungicide to the silica-based plant health promoting additive, respectively.

[0189] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:10 to 10:1 of the melanin synthesis inhibitor fungicide to the silica-based plant health promoting additive, respectively.

[0190] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 20:1 to 4:1 of the melanin synthesis inhibitor fungicide to the silica-based plant health promoting additive, respectively.

[0191] In a preferred embodiment, the components of the combination of the present invention may be mixed in a ratio of 15:1 to 4:1 of the melanin synthesis inhibitor fungicide to the silica-based plant health promoting additive, respectively.

[0192] In one embodiment, the components of the combination of the present invention can be mixed according to the ratios of tricyclazole and orthosilicic acid (1-80):(1-80), (1-70):(1-70), (1-60):(1-60), (1-50):(1-50), (1-40):(1-40), (1-30):(1-30), (1-20):(1-20), (1-10):(1-10), and (1-5):(1-5), respectively.

[0193] In one embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:30 to 30:1 of tricyclazole and orthosilicic acid, respectively.

[0194] In a preferred embodiment, the components of the combination of the present invention can be mixed in a ratio of 1:20 to 20:1 of tricyclazole and orthosilicic acid, respectively.

[0195] In a preferred embodiment, the components of the combination of the present invention can be mixed in a ratio of 1:15 to 15:1 of tricyclazole and orthosilicic acid, respectively.

[0196] In one embodiment, the components of the combination of the present invention may be mixed in a ratio of 1:10 to 10:1 of tricyclazole and orthosilicic acid, respectively.

[0197] In a preferred embodiment, the components of the combination of the present invention can be mixed in a ratio of 20:1 to 4:1 of tricyclazole and orthosilicic acid, respectively.

[0198] In a preferred embodiment, the components of the combination of the present invention can be mixed in a ratio of 15:1 to 4:1 of tricyclazole and orthosilicic acid, respectively.

[0199] Therefore, by referring to the application rate of the active ingredient, the ratio of the systemic fungicide, ie, tricyclazole, to orthosilicic acid can be derived.

[0200] In one embodiment, the present invention provides a method of preparing an agricultural combination comprising a systemic fungicide and a silicate-based plant health promoting additive, the method comprising mixing the ingredients in a desired proportion.

[0201] In one embodiment, the present invention provides a method of preparing an agricultural combination comprising a melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, the method comprising mixing the ingredients in a desired proportion.

[0202] In one embodiment, the present invention provides a method of preparing an agricultural combination comprising tricyclazole and orthosilicic acid, the method comprising mixing the components in a desired proportion.

[0203] In one embodiment, the combination of the present disclosure may comprise at least a third fungicide, herbicide, miticide, biostimulant or insecticide or biological agent.

[0204] In one embodiment, the third fungicide is a combination of at least two fungicides.

[0205] In this embodiment, these fungicides are referred to herein as the third and fourth fungicides, respectively. However, the third and fourth fungicides are preferably not the same fungicides, although they can be a combination of two fungicides from the same class of fungicides.

[0206] In one embodiment, the combination of the present invention may additionally contain plant health promoting additives such as fertilizers, micronutrients, biological additives, etc., which are known to enhance plant growth and health.

[0207] All features described herein may be combined in any combination with any of the above aspects.

[0208] In one embodiment, the components of the combination of the present invention may be tank mixed and sprayed on the infection site, or may be mixed with a surfactant or adjuvant and then sprayed.

[0209] In one embodiment, the ingredients of the combination of the invention may be used for foliar application, ground application or application to plant propagation material.

[0210] In one embodiment, the present disclosure provides an agricultural composition comprising a systemic fungicide and a silica-based plant health promoting additive.

[0211] In one embodiment, the present disclosure provides a systemic fungicide comprising a melanin synthesis inhibitor fungicide.

[0212] In one embodiment, the present disclosure provides an agricultural composition comprising

[0213] melanin synthesis inhibitor fungicides, including isobenzofuranones including fthalide; pyrroloquinolinones including pyroquilon; triazolothiazoles including tricyclazole; cyclopropanecarboxamides including carpropamid; carboxamides including diclocymet; propionamides including fenoxanil; trifluoroethylcarbamates including tolprocarb and mixtures thereof; and

[0214] at least one silicate-based plant health promoting additive; and

[0215] An agriculturally acceptable excipient.

[0216] In one embodiment, the systemic fungicide comprises a demethylation inhibitor fungicide.

[0217] In one embodiment, the demethylation inhibitor fungicide comprises a triazole fungicide.

[0218] In one embodiment, the triazole fungicide is selected from the group consisting of oxepiconazole, oxadiazole, cyproconazole, benzyltriazole, difenoconazole, diniconazole, R-diniconazole, fluconazole, eticonazole, fenbuconazole, fluconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furoconazole, hexaconazole, imipenem, picroconazole, isotrifluconazole, clofoconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, quinconazole, silyfloxacin, tebuconazole, tetraconazole, thiabendazole, triadimefon, triadimenol, triathione, uniconazole and uniconazole, their salts or esters, or isomers or derivatives, or combinations thereof.

[0219] In one embodiment, the silicate-based additive is selected from the group consisting of metasilicic acid (H2SiO3), orthosilicic acid (H4SiO4), disilicate (H2Si2O5) and pyrosilicate (H6Si2O7); orthosilicic acid is preferred.

[0220] In a preferred embodiment, the plant growth promoting additive comprises orthosilicic acid (H4SiO4).

[0221] In another preferred embodiment, the plant growth promoting additive comprises stabilized orthosilicic acid (H4SiO4).

[0222] In one embodiment, the present disclosure provides an agricultural composition comprising

[0223] a melanin synthesis inhibitor fungicide comprising a pyrroloquinolinone;

[0224] Orthosilicic acid; and

[0225] An agriculturally acceptable excipient.

[0226] In one embodiment, the present disclosure provides an agricultural composition comprising

[0227] Tricyclazole;

[0228] Orthosilicic acid; and

[0229] An agriculturally acceptable excipient.

[0230] In one embodiment, the present disclosure provides an agricultural composition comprising

[0231] Tricyclazole;

[0232] Stabilized orthosilicic acid as a silicate-based plant health promoting additive; and

[0233] An agriculturally acceptable excipient.

[0234] In one embodiment, the composition of the present invention can be prepared by mixing the active substance in the composition with an inert carrier and adding surfactant and other adjuvants and carriers as required, and is formulated into solid or liquid preparations, including but not limited to wettable powders, granules, powders, soluble (liquid) concentrates, suspension concentrates, oil-in-water emulsions, water-in-oil emulsions, emulsifiable concentrates, capsule suspensions, ZC preparations, nano preparations, microdispersions, oil dispersions or other known formulation types. The composition can also be used to process plant propagation materials such as seeds, etc. The combination of the present disclosure can be formulated into the form of a composition.

[0235] Examples of solid carriers used in the formulations include granules or powders, such as minerals (e.g., kaolin, attapulgite clay, bentonite, montmorillonite, acid white clay, pyrophyllite, talc, diatomaceous earth and calcite); natural organic materials (e.g., corn cob meal and walnut shell meal); synthetic organic materials (e.g., urea); salts (e.g., calcium carbonate and ammonium sulfate); synthetic inorganic materials (e.g., synthetic hydrated silica); and liquid carriers, aromatic hydrocarbons (e.g., xylene, alkylbenzenes and methylnaphthalene); alcohols (e.g., 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether); ketones (e.g., acetone, cyclohexanone and isophorone); vegetable oils (e.g., soybean oil and cottonseed oil); petroleum aliphatic hydrocarbons, esters, dimethyl sulfoxide, acetonitrile and water.

[0236] Examples of the surfactant include anionic surfactants (such as alkyl sulfate ester salts, alkyl aryl sulfonates, dialkyl sulfosuccinates, polyoxyethylene alkyl aryl ether phosphate salts, lignin sulfonates and naphthalene sulfonate formaldehyde condensates); nonionic surfactants (such as polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl polyoxypropylene block copolymers and sorbitan fatty acid esters) and cationic surfactants (such as alkyl trimethyl ammonium salts).

[0237] Examples of other formulation adjuvants include water-soluble polymers (such as polyvinyl alcohol and polyvinyl pyrrolidone), polysaccharides (such as xanthan gum, alginic acid and its salts, CMC (carboxymethyl cellulose), xanthan gum), inorganic materials (such as magnesium aluminum silicate and alumina sol), preservatives, colorants and stabilizers (such as PAP (isopropyl acid phosphate) and BHT).

[0238] In one embodiment, the composition comprises from about 0.1% to about 50% w / w, preferably from about 1% to about 40% w / w, of an anionic surfactant based on the total weight of the composition.

[0239] In one embodiment, the composition further comprises one or more antifreeze agents, wetting agents, fillers, surfactants, anti-caking agents, pH adjusters, preservatives, biocides, antifoaming agents, colorants, and other formulation aids.

[0240] Suitable antifreeze agents which may be added to the agricultural compositions are liquid polyols, such as ethylene glycol, propylene glycol or glycerol.

[0241] Wetting agents that may be added to the agricultural chemical compositions of the present invention include, but are not limited to, polyaryl alkoxylated phosphates and their potassium salts (e.g., Soprophor ® FLK and Stepfac TSP PE- K). Other suitable wetting agents include dioctyl sodium sulfosuccinate (e.g., Geropon ® SDS and Aerosol ® OT) and ethoxylated alcohols (e.g., Trideth-6; Rhodasurf ® BC 610 and Tersperse ® 4894).

[0242] Optionally, from about 0.1 wt% to about 5.0 wt% of an antifoam or defoaming agent is used to prevent any unwanted foaming generated when making the highly concentrated liquid biocide dispersion composition. The preferred antifoaming agent is selected from the group consisting of silicone compounds, alcohols, glycol ethers, mineral oils, acetylene glycols, polysiloxanes, organosiloxanes, siloxane diols, reaction products of silicon dioxide and organosiloxane polymers, polydimethylsiloxanes or polyalkylene glycols, alone or in combination. Suitable defoaming agents include SAG-10; SAG-1000AP; SAG-1529; SAG-1538; SAG-1571; SAG-1572; SAG-1575; SAG-2 001; SAG-220; SAG-290; SAG-30; SAG-30E; SAG-330; SAG-47; SAG-5440; SAG-7133 and SAG-770.

[0243] Examples of thickeners based on anionic heteropolysaccharides from the xanthan group are, inter alia, Rhodopol 23®, Rhodopol G®, Rhodopol 50 MD®, Rhodicare T®, Kelzan®, Kelzan S® and Satiaxane CX91®.

[0244] Preservatives used can be benzisothiazolinone (Proxel GXL) or polyphenols (phonols), 2-bromo-2-nitropropane-1,3-diol (Bioban BP 30), 5-chloro-2-methyl-4-isothiazolin-3-one and 2-methyl-4-isothiazolin-3-one (Kathon CG / ICP), glutaraldehyde (Ucarcide 50), chloromethylisothiazolinone (CMIT) / methylisothiazolinone (MIT) (Isocil Ultra 1.5), 2,2-dibromo-3-nitrilopropionamide (Reputain 20), natamycin and nisin (Nisin), bronopol / CMIT / MIT (Mergal 721K3).

[0245] Suitable colorants (eg, red, blue and green) are preferably pigments that are sparingly soluble in water and water-soluble dyes. Examples include inorganic colorants (eg, iron oxide, titanium oxide and iron hexacyanoferrate) and organic colorants (eg, alizarin, azo and colorants).

[0246] The filler may include organic or inorganic solid inert substances such as talc, clay, diatomaceous earth, magnesium aluminum silicate, white carbon, pyrophyllite, light calcium carbonate, high clay, organic bentonite, etc. and mixtures thereof.

[0247] The combination of the present disclosure can be sold as a premixed composition or a kit of parts so that the individual active ingredients can be mixed before spraying. Alternatively, the kit can contain a premixed melanin synthesis inhibitor and a plant growth promoter, and a third active substance can be mixed with an adjuvant so that the two components can be mixed before spraying.

[0248] In one embodiment, the present disclosure provides the use of an agricultural combination comprising a systemic fungicide and a silica-based plant health-promoting additive for reducing the application dosage of tricyclazole in crop plantations; synergistically inhibiting fungal growth; significantly reducing the residual level of tricyclazole in rice plantations and field mud; and improving crop yield, nutrition and agricultural product health.

[0249] In one embodiment, the systemic fungicide comprises a melanin synthesis inhibitor fungicide.

[0250] In one embodiment, the present disclosure provides the use of an agricultural combination comprising tricyclazole and a silicate-based plant health promoting additive for reducing the application dosage of tricyclazole in crop plantations; synergistically inhibiting fungal growth; significantly reducing the residual level of tricyclazole in rice plantations and field mud; and improving crop yield, nutrition and agricultural product health.

[0251] In one embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations, and field mud.

[0252] In one embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling rice diseases in a rice plantation.

[0253] In one embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling rice diseases caused by Magnaporthe spp., Magnaporthe grisea, Cochliobolus sp, Cochliobolus miyabeanus, Rhizoctonia sp, Rhizoctonia solani, Gibberella spp., and Gibberella fujikuroi.

[0254] In one embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling rice blast caused by Magnaporthe grisea, Helminthosporium leaf spot caused by Cochliobolus miyabeanus, sheath blight caused by Rhizoctonia solani, and seedling blight caused by Gibberella fujikuroi.

[0255] In a preferred embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling rice diseases such as blast caused by Magnaporthe grisea.

[0256] In a preferred embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling leaf blight.

[0257] In a preferred embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling neck blight.

[0258] In a preferred embodiment, the present disclosure provides a use of an agricultural combination comprising tricyclazole and orthosilicic acid for controlling rice blast caused by Magnaporthe grisea in basmati rice.

[0259] The methods of the present invention can be used to control a wide range of plant diseases, such as:

[0260] In one embodiment, the present fungicide composition can be used to control fungal diseases, such as pea diseases caused by one or more of the following species: Alternaria alternata, Aphanomyces euteiches f. sp. pisi, Ascochyta pinodella, Ascochyta pinodes, Ascochyta pisi, Botryotinia fuckeliana, Botrytis cinerea, Cercosporapisa-sativae, Chalara elegans, Cladosporium cladosporioides f. sp. pisicoia, Cladosporium pisicoia, Colletotrichum gloeosporioides, Colletotrichum pisi), Erysiphe pisi, Fusarium oxysporum, Fusariumoxysporum. sp. pisi, Fusarium solani, Fusicladiumpisicoia, Glomerella cingulata, Mycosphaerellapinodes, Oidium sp., Peronospora viciae, Phomapinodella, Pythium spp., Rhizoctonia solani, Sclerotinia sclerotiorum, Septoria pisi, Thanatephonis cucumeris, Thielaviopsis hasicola, and Uromyces rust fabae), soybean diseases: Cercospora kikuchii, Elsinoe glycines, stem blight (Diaporthephaseolorum var.sojae), Septoria glycines, Cercospora sojina, Phakopsora pachyrhizi, Phytophthora sojae, and Rhizoctonia solani.

[0261] Wheat diseases: powdery mildew (Erysiphe graminis), fusarium ear blight (Fusarium graminearum, F. avenacerum, F. culmorum, Microdochium nivale), rust (Puccinia striiformis, P. graminis, P. cingulate), snow mold (Micronectriella nivale), snow rot (Typhula spp.), loose smut (Ustilago tritici), wheat grain disease (Tilletia caries), eye spot (Pseudocercosporella herpotrichoides), leaf spot (Mycosphaerella graminicola), glume spot (Stagonospora nodorum), and yellow spot (Pyrenophora tritici-repentis).

[0262] Barley diseases: Powdery mildew (Erysiphe graminis), Fusarium ear blight (Fusarium graminearum), Fusarium avenacerum, Fusarium culmorum, Microdochium nivale), Rust (Puccinia striiformis, P. graminis, P. hordei), Loose smut (Ustilago nuda), Scald (Rhynchosporium secalis), Web blotch (Pyrenophorateres), Spot (Cochliobolus sativus), Sheath blight (Pyrenophora graminea) and Rhizoctonia rot (Rhizoctonia solani)

[0263] Corn diseases: Ustilago (Ustilago maydis), brown spot (Cochliobolus heterostrophus), copper spot (Gloeocercospora sorghi), southern rust (Puccinia polysora), gray spot (Cercosporazeae-maydis), white spot (Phaeosphaeria mydis and / or Pantoea ananatis), and rhizoctonia rot (Rhizoctonia solani).

[0264] Citrus diseases: Melanosis (Diaporthe citri), scab (Elsinoe fawcetti), penicillium rot (Penicillium digitatum, P. italicum), and brown rot (Phytophthora parasitica, Phytophthora citrophthora).

[0265] Apple diseases: blossom end rot (Monilinia mali), canker (Valsa ceratosperma), powdery mildew (Podosphaera leucotricha), alternaria leaf spot (Alternaria altemata apple pathotype), scab (Venturia inaequalis), powdery mildew, bitter rot (Colletotrichum acutatum), crown rot (Phytophtora cactorum), spot (Diplocarpon malí) and ring rot (Botryosphaeria berengeriana).

[0266] Pear diseases: scab (Venturia nashicola, V. pirina), powdery mildew, black spot (Alternaria alternata), rust (Gymnosporangium haraeanum), and phytophthora fruit rot (Phytophtora cactorum).

[0267] Peach diseases: brown rot (Monilinia fructicola), powdery mildew, scab (Cladosporium carpophilum), and mold rot (Phomopsis sp.).

[0268] Grapevine diseases: Anthracnose (Elsinoe ampelina), late rot (Glomerella cingulate), powdery mildew (Uncinula necator), rust (Phakopsora ampelopsidis), black rot (Guignardia bidwellii), gray mold and downy mildew (Plasmopara vitícola).

[0269] Diseases of Japanese persimmon: Anthracnose (Gloeosporiumkaki) and leaf spot (Cercospora kaki, Mycosphaerella nawae).

[0270] Cucurbit diseases: Anthracnose (Colletotrichum lagenarium), powdery mildew (Sphaerotheca fuliginea), vine blight (Mycosphaerella melonis), Fusarium wilt (Fusarium oxysporum), downy mildew (Pseudoperonospora cubensis), blight (Phytophthora spp.), and damping-off (Pythium spp.).

[0271] Tomato diseases: early blight (Alternaria solani), leaf mold (Cladosporium fulvum), and late blight (Phytophthora infestans)

[0272] Eggplant diseases: Brown spot (Phomopsis vexans) and powdery mildew (Erysiphe cichoracearum). Cruciferous vegetable diseases: Alternaria leaf spot (Alternaria japonica), white spot (Cercosporella brassicae), clubroot (Plasmodiophora brassicae) and downy mildew (Peronospora parasitica).

[0273] Onion diseases: Rust (Puccinia allii) and downy mildew (Peronospora destructor).

[0274] Bean diseases: Purple seed spot (Cercospora kikuchii), black bean disease (Elsinoe glycines), pod and stem blight (Diaporthe phaseolorum var. sojae), black spot (Septoria glycines), gray spot (Cercospora sojina), rust (Phakopsora pachyrhizi), yellow rust, brown stem rot (Phytophthora sojae), and rhizoctonia rot (Rhizoctonia solani).

[0275] Kidney bean diseases: Anthracnose (Colletotrichum lindemthianum). Peanut diseases: Leaf spot (Cercospora personata), Brown spot (Cercosporaarachidicola), and White rot (Sclerotium rolfsii).

[0276] Pea diseases: Powdery mildew (Erysiphe pisi) and root rot (Fusarium solani f. sp. pisi).

[0277] Potato diseases: Early blight (Alternaria solani), late blight (Phytophthora infestans), pink rot (Phytophthora erythroseptica), and powdery scab (Spongospora subterranean f. spp. Glomerella cingulate).

[0278] Strawberry diseases: Powdery mildew (Sphaerotheca humuli) and anthracnose (Glomerella cingulata).

[0279] Tea plant diseases: blister blight (Exobasidium reticulatum), white scab (Elsinoe leucospila), gray blight (Pestalotiopsis spp.), and anthracnose (Colletotrichum theae-sinensis).

[0280] Tobacco diseases: Brown spot (Alternaria longipes), powdery mildew (Erysiphe cichoracearum), anthracnose (Colletotrichum tabacum), downy mildew (Peronospora tabacina), and blackleg (Phytophthora nicotianae).

[0281] Rapeseed diseases: Sclerotinia sclerotiorum and Rhizoctonia rot (Rhizoctonia solani). Cotton diseases: Rhizoctonia rot (Rhizoctonia solani).

[0282] Sugar beet diseases: Cercospora leaf spot (Cercospora beticola), leaf blight (Thanatephorus cucumeris), root rot (Thanatephorus cucumeris), and Aphanomyces root rot (Aphanomyces cochlioides).

[0283] Diseases of roses: black spot (Diplocarpon rosae), powdery mildew (Sphaerotheca pannosa) and downy mildew (Peronospora sparsa), diseases of chrysanthemums and Asteraceae: downy mildew (Bremia lactucae), leaf blight (Septoria chrysanthemi-indici) and white rust (Puccinia horiana).

[0284] Diseases: Diseases caused by Pythium fungi (Pythium aphanidermatum, Pythium debarianum, Pythium graminicola, Pythium irregulare, Pythium ultimum, gray mold (Botrytis cinerea) and sclerotinia (Sclerotinia sclerotiorum).

[0285] Diseases of Japanese radish: Alternaria leaf spot (Alternaria brassicicola).

[0286] Turfgrass diseases: round spot (Sclerotinia homeocarpa), brown spot, and large spot (Rhizoctonia solani.

[0287] Banana diseases: Black Sigatoka (Mycosphaerella fijiensis), Yellow Sigatoka (Mycosphaerella musicola).

[0288] Sunflower diseases: downy mildew (Plasmopara halstedii).

[0289] Seed diseases or various early plant growth diseases caused by Aspergillus spp., Penicillium spp., Fusarium spp., Gibberella spp., Tricoderma spp., Thielaviopsis spp., Rhizopus spp., Mucor spp., Corticium spp., Phoma spp., Rhizoctonia spp. and Diplodia spp.

[0290] Viral diseases of various plants mediated by Polymixa spp. or Olpidium spp.

[0291] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of the fungicide in agricultural products, rice plantations and field mud, the agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 60% of the fungicidal effective amount of the fungicide.

[0292] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of the fungicide in agricultural products, rice plantations and field mud, the agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of the melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 55% of the fungicidal effective amount of the fungicide.

[0293] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of the fungicide in agricultural products, rice plantations and field mud, the agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of the melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 50% of the fungicidal effective amount of the fungicide.

[0294] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of the fungicide in agricultural products, rice plantations and field mud, the agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 40% of the fungicidal effective amount of the fungicide.

[0295] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of the fungicide in agricultural products, rice plantations and field mud, the agricultural combination comprising at least one melanin synthesis inhibitor fungicide and a silicate-based plant health promoting additive, wherein the amount of the melanin synthesis inhibitor fungicide in the combination is reduced by about 1% to about 30% of the fungicidal effective amount of the fungicide.

[0296] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations and field mud, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of its fungicidally effective amount.

[0297] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations and field mud, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 55% of its fungicidally effective amount.

[0298] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations and field mud, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50% of its fungicidal effective amount.

[0299] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations and field mud, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 40% of its fungicidally effective amount.

[0300] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in agricultural products, rice plantations and field mud, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 30% of its fungicidally effective amount.

[0301] In one embodiment, the present disclosure provides use of an agricultural combination for significantly reducing the residual level of tricyclazole in a basmati rice product, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of its fungicidally effective amount.

[0302] In a preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in a basmati rice product, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 55% of its fungicidally effective amount.

[0303] In another preferred embodiment, the present disclosure provides use of an agricultural combination for significantly reducing the residual level of tricyclazole in a basmati rice product, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50% of its fungicidally effective amount.

[0304] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in a basmati rice product, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 40% of its fungicidal effective amount.

[0305] In another preferred embodiment, the present disclosure provides the use of an agricultural combination for significantly reducing the residual level of tricyclazole in a basmati rice product, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 30% of its fungicidal effective amount.

[0306] The present invention also provides the use of the present agricultural combination for protecting plant propagation materials (preferably seeds) and roots and shoots of seedlings from plant pathogenic fungi, which comprises contacting plant propagation materials (such as seeds) with the composition of the present disclosure before sowing and / or before germination.

[0307] The present invention provides a method of use for alleviating the problems associated with residues of systemic fungicides (i.e., tricyclazoles found in farm mud, soil, and even in produce from crops farmers treat with such systemic fungicides). Pesticide residues negatively impact the export prospects of crop products, ultimately undermining the economic benefits that could otherwise be generated, given restrictions imposed by regulatory agencies.

[0308] In one embodiment, the present disclosure is directed to a method of reducing systemic fungicide residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising a systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of systemic fungicide in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of the systemic fungicide.

[0309] In one embodiment, the present disclosure is directed to a method of reducing the amount of systemic fungicide applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising a systemic fungicide and a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of the systemic fungicide.

[0310] In one embodiment, the present disclosure is directed to a method of reducing systemic fungicide residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising a systemic fungicide and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 55% of the fungicidally effective amount of the systemic fungicide.

[0311] In one embodiment, the present disclosure is directed to a method of reducing systemic fungicide residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising a systemic fungicide and a silica-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50%, about 1% to 40%, or about 1% to 30% of the fungicidally effective amount of the systemic fungicide.

[0312] In one embodiment, the systemic fungicide of the present disclosure is selected from the group consisting of melanin synthesis inhibitors, demethylation inhibitor fungicides, nucleic acid synthesis inhibitors, cytoskeleton and motor protein inhibitors, amino acid and protein synthesis inhibitors, respiratory process inhibitors, signal transduction inhibitors, lipid synthesis and membrane integrity disruptors, sterol biosynthesis inhibitors, cell wall biosynthesis inhibitors, host plant defense inducers and / or fungicides with unknown modes of action.

[0313] In one embodiment, the melanin synthesis inhibitor fungicide comprises isobenzofuranones, the isobenzofuranones comprising fthalide; pyrroloquinolinones, the pyrroloquinolinones comprising pyroquilon; triazolothiazoles, the triazolothiazoles comprising tricyclazole; cyclopropanecarboxamides, the cyclopropanecarboxamides comprising carpropamid; carboxamides, the carboxamides comprising diclocymet; propionamides, the propionamides comprising fenoxanil; trifluoroethylcarbamates, the trifluoroethylcarbamates comprising tolprocarb and mixtures thereof; and

[0314] In one embodiment, the demethylation inhibitor fungicide comprises a triazole fungicide.

[0315] In one embodiment, the triazole fungicide is selected from the group consisting of oxepiconazole, oxadiazole, cyproconazole, benzyltriazole, difenoconazole, diniconazole, R-diniconazole, fluconazole, eticonazole, fenbuconazole, fluconazole, fluquinconazole, flusilazole, flutriafol, furconazole, furoconazole, hexaconazole, imipenem, picroconazole, isotrifluconazole, clofoconazole, metconazole, myclobutanil, penconazole, propiconazole, prothioconazole, quinconazole, silyfloxacin, tebuconazole, tetraconazole, thiabendazole, triadimefon, triadimenol, triathione, uniconazole and uniconazole, their salts or esters, or isomers or derivatives, or combinations thereof.

[0316] In one embodiment, the present disclosure relates to a method for reducing the application of a melanin synthesis inhibitor fungicide in a crop plantation, the method comprising applying to a site a fungicidally effective amount of an agricultural combination comprising the fungicide and a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of the fungicide.

[0317] In one embodiment, the present disclosure relates to a method for reducing the residue of a melanin synthesis inhibitor fungicide in a crop plantation, the method comprising applying to a site a fungicidally effective amount of an agricultural combination comprising the fungicide and a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 55% of the fungicidally effective amount of the fungicide.

[0318] In one embodiment, the present disclosure relates to a method for reducing the residue of a melanin synthesis inhibitor fungicide in a crop plantation, the method comprising applying to a site a fungicidally effective amount of an agricultural combination comprising the fungicide and a silicate-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50%, about 1% to 40% or about 1% to 30% of the fungicidally effective amount of the fungicide.

[0319] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole.

[0320] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 55% of the fungicidally effective amount of tricyclazole.

[0321] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50% of the fungicidally effective amount of tricyclazole.

[0322] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 40% of the fungicidally effective amount of tricyclazole.

[0323] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 30% of the fungicidally effective amount of tricyclazole.

[0324] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole.

[0325] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 55% of the fungicidally effective amount of tricyclazole.

[0326] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and an orthosilicic acid promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 50% of the fungicidally effective amount of tricyclazole.

[0327] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 40% of the fungicidally effective amount of tricyclazole.

[0328] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole residues in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 30% of the fungicidally effective amount of tricyclazole.

[0329] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 1 gai / ha to 70 g ai / ha.

[0330] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0331] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 290 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 50 g ai / ha.

[0332] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 285 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0333] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 270 g ai / ha and orthosilicic acid in an amount ranging from 20 gai / ha to 35 g ai / ha.

[0334] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 250 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0335] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0336] The agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 1 g ai / ha to 70 g ai / ha.

[0337] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0338] The agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 290 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 50 g ai / ha.

[0339] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0340] The agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 285 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0341] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0342] The agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 270 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0343] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0344] The agricultural combination comprises tricyclazole in an amount ranging from 150 g ai / ha to 250 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0345] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0346] The agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 1 g ai / ha to 70 g ai / ha.

[0347] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0348] The agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 290 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 50 g ai / ha.

[0349] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0350] The agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 285 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0351] In one embodiment, the present disclosure is directed to a method of reducing the amount of tricyclazole applied in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid,

[0352] The agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 250 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0353] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 150 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0354] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 187 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0355] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 225 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0356] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 243 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0357] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 262 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0358] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 281 g ai / ha of tricyclazole and 25 g ai / ha of orthosilicic acid.

[0359] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 150 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0360] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 187 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0361] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 225 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0362] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 243 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0363] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 262 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0364] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises 281 g ai / ha of tricyclazole and 30 g ai / ha of orthosilicic acid.

[0365] In one embodiment, the agricultural combination comprising tricyclazole and orthosilicic acid is applied as a tank mix.

[0366] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination as a tank mix, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole at an application rate ranging from 50 g / ha to 300 g / ha and orthosilicic acid at an application rate ranging from 250 ml / ha to 2500 ml / ha.

[0367] In one embodiment, the present disclosure relates to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination as a tank mix, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole at an application rate ranging from 100 g / ha to 400 g / ha and orthosilicic acid at an application rate ranging from 500 ml / ha to 2000 ml / ha.

[0368] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination as a tank mix, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole at an application rate ranging from 100 g / ha to 400 g / ha and orthosilicic acid at an application rate ranging from 1000 ml / ha to 2000 ml / ha.

[0369] In one embodiment, the present disclosure is directed to a method of reducing tricyclazole application in a crop plantation, the method comprising applying to a locus a fungicidally effective amount of an agricultural combination as a tank mix, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the agricultural combination comprises tricyclazole at an application rate ranging from 200 g / ha to 380 g / ha and orthosilicic acid at an application rate ranging from 1000 ml / ha to 1800 ml / ha.

[0370] In one embodiment, the present disclosure is directed to a method of improving yield, plant health and quality, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 60% of the fungicidally effective amount of tricyclazole.

[0371] In one embodiment, the present disclosure relates to a method of improving rice yield, plant health and quality, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 55% of the fungicidally effective amount of tricyclazole.

[0372] In one embodiment, the present disclosure relates to a method of improving rice yield, plant health and quality, wherein the amount of tricyclazole in the combination is reduced from about 1% to about 55% of the fungicidally effective amount of tricyclazole.

[0373] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to an infection site, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and a silicic acid-based plant health promoting additive, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole.

[0374] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to a plant locus, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole, wherein the agricultural combination comprises tricyclazole in an amount ranging from 50 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 1 g ai / ha to 70 g ai / ha.

[0375] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to an infection site, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 300 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0376] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to an infection site, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 290 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0377] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to an infection site, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole, wherein the agricultural combination comprises tricyclazole in an amount ranging from 100 g ai / ha to 250 g ai / ha and orthosilicic acid in an amount ranging from 10 g ai / ha to 40 g ai / ha.

[0378] In another embodiment, the present disclosure is directed to a method of controlling plant pathogenic fungi comprising applying to an infection site, plant, plant part, plant propagation material, pest, their food supply, habitat or breeding ground a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of tricyclazole, wherein the agricultural combination comprises tricyclazole in an amount ranging from 200 g ai / ha to 250 g ai / ha and orthosilicic acid in an amount ranging from 20 g ai / ha to 35 g ai / ha.

[0379] In another embodiment, the present disclosure is directed to a method of controlling the plant pathogen Pyricularia oryzae, comprising applying to an infection site, plant, plant part, plant propagation material in a rice plantation, or to the pest, its food supply, habitat or breeding ground, a fungicidally effective amount of an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole in the combination is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of tricyclazole.

[0380] The combination of the present invention can be applied simultaneously as a tank mix or formulation, or can be applied sequentially. It can be applied to the soil before plant emergence, before planting or after planting. Application can be carried out as a foliar spray at different time points during crop development, once or twice in the early or late stages after emergence.

[0381] The combinations according to the invention can be applied before or after the useful plants or their propagation material have been infected by the fungi.

[0382] According to an embodiment of the present disclosure, a kit comprising an agricultural combination / composition is provided. The kit comprises a plurality of components, the plurality of components comprising the systemic fungicide of the present disclosure and a silicic acid-based plant health promoting additive.

[0383] In one embodiment of the present disclosure, the kit includes at least one or all components. For example, the kit may include additional fungicides, herbicides, insecticides or biostimulants or bioadjuvants, anionic surfactants and nonionic surfactants.

[0384] One or more components may have been combined or preformulated. In those embodiments where more than two components are provided in the kit, the components may have been combined and packaged as is in a single container such as a vial, bottle, can, pouch, bag and / or jar. In other embodiments, two or more components of the kit may be packaged separately, i.e. they are not preformulated. Therefore, the kit may include one or more separate containers, such as vials, cans, bottles, pouches, bags and / or cans, each containing separate components of the stable fungicide composition.

[0385] In both forms, the components of the kit may be applied separately from the other components or together as components of a combination or composition according to the disclosure to prepare an agricultural combination or composition according to the disclosure.

[0386] The present disclosure also provides a kit comprising a fungicide composition for controlling the growth of fungal plant pathogens and instructions for use. The instructions generally comprise instructions for applying the fungicide composition to a plant, its locus, or its plant propagation material.

[0387] According to one embodiment, the kit comprises:

[0388] Systemic fungicide,

[0389] A silicic acid-based plant health-promoting additive, and

[0390] Instructions for use.

[0391] In another embodiment, the kit comprises:

[0392] Melanin synthesis inhibitor fungicide

[0393] A silicic acid-based plant health-promoting additive, and

[0394] and optionally further comprising:

[0395] Instructions for use.

[0396] In a preferred embodiment, the kit comprises:

[0397] Tricyclazole

[0398] Orthosilicic acid

[0399] and optionally further comprises:

[0400] Instructions for use.

[0401] In a preferred embodiment, the kit comprises:

[0402] Tricyclazole

[0403] Stabilized orthosilicic acid

[0404] and optionally further comprises:

[0405] Instructions for use.

[0406] According to the present invention, "improvement of plant quality" means that certain traits are improved qualitatively or quantitatively when compared to the same traits in control plants grown under the same conditions without the method of the present invention. Such traits include, but are not limited to, improved visual appearance of plants, reduced ethylene (reduced yield and / or inhibition of acceptance), improved quality of harvested materials such as seeds, fruits, leaves, vegetables (such improved quality can be manifested as improved visual appearance of harvested materials), etc.

[0407] The composition of the present invention can be applied to the plant site once or multiple times during the growth of the plant. It can be applied to the planting site before sowing seeds, during sowing seeds, before emergence and / or after emergence. The composition can also be used when the plant is grown in a greenhouse and can continue to be used after transplanting. For example, the soil can be treated directly before, during or after transplanting. The use of the composition can be carried out by any suitable method that ensures that the agent penetrates the soil, for example, seedling tray application, furrow application, soil drench, soil injection, drip irrigation, application by a sprinkler or center pivot, incorporation into the soil (wide pouring or in-band) is such a method.

[0408] Plants and plant parts are treated according to the invention directly or with the active compounds or their combinations using customary treatment methods, for example by foliar application, dipping, spraying, atomizing, irrigation, evaporation, dusting, aerosolizing, broadcasting, foaming, painting, spreading, watering (drenching), drip irrigation, and in the case of propagation material, in particular in the case of seeds, additionally as powders for dry seed treatment, solutions for seed treatment, water-soluble powders for slurry treatment, by encrustation, by coating with one or more layers, etc. It is also possible to apply the active compounds in combination with other active substances by the ultra-low volume method or to inject the active compound combinations into the soil.

[0409] In one embodiment, application is by foliar spray.

[0410] The rate and frequency of application of the compositions to plants can vary within wide limits and depend on the type of use, the specific active agent, the nature of the soil, the method of application (pre-emergence or post-emergence, etc.), the plant, the prevailing climatic conditions and other factors governed by the method of application, the time of application and the target plant.

[0411] The method disclosed herein has great advantages over the prior art and conventionally used methods of using tricyclazoles. The method disclosed herein helps to improve the nutritional quality of yields and promote plant health without phytotoxicity.

[0412] The invention will now be described with reference to the following specific examples. It should be noted that the following examples illustrate rather than limit the invention, and that those skilled in the art will be able to design many alternative examples without departing from the scope of the invention.

[0413] Example

[0414] Example 1: Efficacy of tricyclazole combined with orthosilicic acid

[0415] The efficacy of a combination of tricyclazole and orthosilicic acid (OSA) was evaluated by determining the percentage of disease control of rice blast fungus ( Pyricularia oryzae ) in a laboratory trial.

[0416] Protocol: In laboratory experiments, cornmeal agar (CMA) medium (Hi-media) was used as a substrate for the growth of the fungus Pyricularia oryzae to investigate the efficacy of a combination treatment of tricyclazole and orthosilicic acid. CMA medium was prepared and heated to 45 °C before solidification (45 °C). oC) Add the fungicide at the concentration listed in Table 1 (a) below to make the modification. Pour the modified medium evenly, i.e., pour about 18 ml into each 90-mm culture dish. Cut a mycelial disk of about 10 mm from the grown pathogen Pyricularia oryzae and inoculate it in the center of the culture dish containing the medium and incubate at 27 ± 2 o C for 8-10 days. A control dish without fungicide was also set up as a control. The radial growth of mycelium (in millimeters) was measured on the 10th day of cultivation.

[0417] Table 1(a): Regimens evaluating the efficacy of tricyclazole and OSA

[0418]

[0419] All experiments were performed in triplicate. The percent fungal control / percent inhibition of P. oryzae hyphal growth relative to the control was calculated using the following formula.

[0420]

[0421] in,

[0422] Dc = mean diameter of fungal growth in controls.

[0423] Dc = mean diameter of fungal growth in treatment.

[0424] In addition, the synergistic effect of the combination is evaluated and determined using the well-established Colby calculation method. A common approach to improving the use profile of agricultural products is the combination of an active compound with one or more other active compounds that contribute to the desired additional properties. However, when two or more active compounds are used in combination, it is not uncommon for physical and biological incompatibilities to occur, such as insufficient stability of the combined formulation, decomposition of the active compounds or antagonism of the active compounds. On the contrary, what is needed is an active compound combination that has a favorable activity profile, high stability and, if possible, synergistically enhanced activity, thus allowing a reduction in the application rate compared to the individual application of the active compounds to be combined.

[0425] When mixed, chemical mixtures can have antagonistic effects, where the results are less than expected when the chemicals are combined. There can also be additive effects, where the resulting mixture gives the results expected from the sum of its components. Finally, when the results are greater than expected, there may be a synergistic effect. Synergistic results are rare and are usually only observed in results at high concentrations. In the strictly regulated agricultural industry, high concentrations are undesirable in the environment. Therefore, when a synergistic effect is achieved at low doses, the resulting mixture is indeed a rare and unexpected discovery.

[0426] A synergistic effect in agricultural products always exists when the anti-phytopathogenic activity of the active compound combination exceeds the total activity of the active compounds when applied individually. The expected activity of a given combination of two active compounds can be calculated according to SR Colby ("Calculation of synergistic and antagonistic reactions of herbicide combinations", Weeds 15, (1967), 20-22).

[0427] The effects of all treatments were measured as the percentage reduction of Pyricularia oryzae and were evaluated according to the Colby equation as shown below.

[0428] if,

[0429] X is the growth inhibition percentage of tricyclazole (A) at the application rate,

[0430] Y is the percentage of growth inhibition of orthosilicic acid (B) at the application rate, and

[0431] E represents the expected growth percentage of the control with tricyclazole and orthosilicic acid when the active compounds A and B are applied at the specific application rates defined below.

[0432] therefore,

[0433]

[0434] If the actual / observed anti-phytopathogen efficacy of the combination of tricyclazole and orthosilicic acid against Pyricularia oryzae exceeds the calculated value, the activity of the combination is superadditive, i.e. there is a synergistic effect. In this case, the observed efficacy must be greater than the value of the expected efficacy calculated by the above formula.

[0435] The percentage control of Pyricularia oryzae (Rice Blast) by the combination of tricyclazole and orthosilicic acid and tricyclazole and orthosilicic acid alone is shown in Table 1 below:

[0436] Table 1(b): Percentage control of Pyricularia oryzae

[0437]

[0438] Based on the observations in Table 1, the current tricyclazole and orthosilicic acid combination has a synergistic effect and exhibits an exemplary control effect on fungi.

[0439] Example 2: Efficacy of tricyclazole and orthosilicic acid against leaf blight

[0440] A field trial study was conducted to evaluate the bioefficacy and residue of different doses of a combination of tricyclazole and orthosilicic acid (OSA) against rice leaf blast (Pyricularia oryzae) disease. The percentage reduction of tricyclazole application was also determined using tricyclazole alone (i.e., treatment number 2) as the standard dose. The amount of active ingredient is given in g ai / ha in Table 2(a), and the application dose rates of the tank mix are also given in Table 2(a), with tricyclazole in g / ha and OSA in ml / ha. The foliar application of this agricultural combination was applied at 10-day intervals with 3 replications.

[0441] The first application of the combination of tricyclazole and orthosilicic acid at time 0 was denoted as "A", the application 10 days after application "A" was denoted as "B", and the application 10 days after application "B" was denoted as "C". Leaf blight control was observed 10 days after the three applications (i.e., 10 DAA, 10 DAB, and 10 DAC).

[0442] Table 2(a): Treatment regimens and percentage reduction of tricyclazole (%)

[0443]

[0444] WP: Wettable Powder

[0445] Note: The percentage reduction in tricyclazole dose was calculated with reference to the standard dose of tricyclazole alone (treatment number 2).

[0446] Table 2(b): Observation results of rice leaf blast control

[0447]

[0448] The letters a, b, c, d, and e indicate statistical differences between the values.

[0449] Field trials were conducted in rice plantations using an agricultural combination consisting of tricyclazole and orthosilicic acid, wherein the dosage and application rate of tricyclazole were significantly reduced compared to the application of tricyclazole alone. Thus, it was observed that the control percentage of the rice blast fungus (Pyricularia oryzae) by the agricultural combination consisting of tricyclazole and orthosilicic acid was comparable to or even better than that of tricyclazole alone.

[0450] Therefore, the application of a combination of tricyclazole and orthosilicic acid, in which the amount of tricyclazole is reduced, can effectively control rice leaf blast.

[0451] Example 3: Efficacy of tricyclazole and orthosilicic acid against rice neck blast

[0452] A field trial study was conducted to evaluate the bioefficacy and residue of different doses of a combination consisting of tricyclazole and orthosilicic acid against rice neck blast (Pyricularia oryzae) disease.

[0453] The percentage reduction in the agricultural application rate of tricyclazole was also determined using tricyclazole alone (i.e., treatment number 2) as the standard dose. The amount of active ingredient is listed in g ai / ha in Table 3(a), and the application rate of the tank mix is ​​also listed in Table 3(a), with tricyclazole in g / ha and OSA in ml / ha.

[0454] The foliar application of this agricultural combination was repeated 3 times at an interval of 10 days. The first application of the combination of tricyclazole and orthosilicic acid (OSA) at time 0 was denoted as "A", and the application 10 days after the application of "A" was denoted as "B". The percentage of disease control was observed 20 days after the second application of B, i.e. 20 DAB.

[0455] Table 3(a): Treatment regimens and percentage reduction of tricyclazole (%)

[0456]

[0457] WP: Wettable Powder

[0458] Note: The percentage reduction in tricyclazole dose was calculated with reference to the standard dose of tricyclazole alone (treatment number 2).

[0459] Table 3(b): Observation results of rice neck blast control

[0460]

[0461] The letters a, b, c, d, and e indicate statistical differences between the values.

[0462] A field trial study was conducted in a rice plantation to determine the reduction of the neck blast disease by applying an agricultural combination consisting of tricyclazole and orthosilicic acid, wherein the dosage and application rate of tricyclazole were significantly reduced compared to the application of tricyclazole alone. Thus, it was observed that the application of the agricultural combination consisting of tricyclazole and orthosilicic acid was better than the application of tricyclazole alone in terms of the percentage of control of the rice blast disease.

[0463] Therefore, the application of a combination of tricyclazole and orthosilicic acid, in which the amount of tricyclazole used is significantly reduced compared to when tricyclazole is used alone, can effectively control rice neck blast.

[0464] Example 4: Efficacy of tricyclazole and orthosilicic acid against cervical erythema

[0465] A field trial study was conducted to evaluate the bioefficacy and residue of different doses of a combination consisting of tricyclazole and orthosilicic acid (OSA) as a tank mix against rice neck blast (Pyricularia oryzae) disease. The percentage reduction of tricyclazole application rate was also determined. The foliar application of this agricultural combination was applied at 10-day intervals with 3 replications.

[0466] The percentage reduction in the agricultural application rate of tricyclazole was also determined using tricyclazole alone (i.e., treatment number 2) as the standard dose. The amount of active ingredient is listed in g ai / ha in Table 4(a), and the application rate of the tank mix is ​​also listed in Table 4(a), with tricyclazole in g / ha and OSA in ml / ha.

[0467] The first application of the combination of tricyclazole and orthosilicic acid (OSA) at time 0 is denoted as "A" and the application 10 days after application "A" is denoted as "B". The percent control of the disease was observed 10 days after each application of A, B and C, i.e., 10DA-A, 10DA-B and 10DA-C.

[0468] Table 4(a): Treatment regimens and percentage reduction of tricyclazole (%)

[0469]

[0470] WP: Wettable Powder

[0471] Note: The percentage reduction in tricyclazole dose was calculated with reference to the standard dose of tricyclazole alone (treatment number 2).

[0472] Table 4(b): Observation results of rice blast control

[0473]

[0474] The letters a, b, c, d, and e indicate statistical differences between the values.

[0475] It is evident from Table 4 that a 50% reduction in the amount of tricyclazole (Treatment Nos. 4 and 8) compared to the standard dose, effectively controlled Pyricularia oryzae and the percentage of control of the plant pathogen was comparable to the percentage of control of the disease when the standard dose of tricyclazole was used alone (Treatment No. 2). Similarly, when the percentage of tricyclazole was reduced by 37.5%, 25% and 12.5% ​​(Treatment Nos. 5, 6, 7, 9, 10 and 11), effective and enhanced control of the pathogen was observed compared to the standard dose (Treatment No. 2).

[0476] Field trials were conducted in rice plantations using an agricultural combination consisting of tricyclazole and orthosilicic acid, wherein the dosage and application rate of tricyclazole were significantly reduced compared to the application of tricyclazole alone. Thus, it was observed that the control percentage of the rice blast fungus (Pyricularia oryzae) by the agricultural combination consisting of tricyclazole and orthosilicic acid was comparable to or even better than that of tricyclazole alone.

[0477] Therefore, the present invention plays a vital role in agricultural practice in protecting human welfare, soil flora and environmental health, as well as helping to reduce the accumulation of pesticide residues and their potential harmful effects.

Claims

1. An agricultural combination, comprising: melanin synthesis inhibitor fungicides, the melanin synthesis inhibitor fungicides comprising isobenzofuranones, the isobenzofuranones comprising fthalide; pyrroloquinolinones, the pyrroloquinolinones comprising pyroquilon; triazolothiazoles, the triazolothiazoles comprising tricyclazole; cyclopropanecarboxamides, the cyclopropanecarboxamides comprising carpropamid; carboxamides, the carboxamides comprising diclocymet; propionamides, the propionamides comprising fenoxanil; trifluoroethylcarbamates, the trifluoroethylcarbamates comprising tolprocarb, and mixtures thereof; and at least one silicate-based plant health promoting additive; The amount of the melanin synthesis inhibitor fungicide is reduced by about 1% to about 60% of its fungicidally effective amount.

2. The combination according to claim 1, wherein the melanin synthesis inhibitor fungicide is selected from the group consisting of triazolothiazole fungicides comprising tricyclazole.

3. The combination of claim 1, wherein the silicate-based plant health promoting additive comprises orthosilicic acid (H4SiO4).

4. The combination of claim 1, wherein the ratio of the melanin synthesis inhibitor fungicide to the silicate-based plant health promoting additive ranges from 1:20 to 20:

1.

5. The combination of claim 1, wherein the ratio of the melanin synthesis inhibitor fungicide to the silicate-based plant health promoting additive ranges from 1:15 to 15:

1.

6. The combination of claim 1 comprising tricyclazole and orthosilicic acid.

7. The combination of claim 1, wherein the amount of the melanin synthesis inhibitor fungicide is reduced by at least about 1% to at least about 60% of its fungicidally effective amount.

8. The combination of claim 1, wherein the amount of the melanin synthesis inhibitor fungicide is reduced by at least about 1% to at least about 55% of its fungicidally effective amount.

9. The combination of claim 1, wherein the combination further comprises one or more fungicides, herbicides, insecticides or biological agents.

10. An agricultural composition, comprising: melanin synthesis inhibitor fungicides, the melanin synthesis inhibitor fungicides comprising isobenzofuranones, the isobenzofuranones comprising fthalide; pyrroloquinolinones, the pyrroloquinolinones comprising pyroquilon; triazolothiazoles, the triazolothiazoles comprising tricyclazole; cyclopropanecarboxamides, the cyclopropanecarboxamides comprising carpropamid; carboxamides, the carboxamides comprising diclocymet; propionamides, the propionamides comprising fenoxanil; trifluoroethylcarbamates, the trifluoroethylcarbamates comprising tolprocarb, and mixtures thereof; and at least one silicate-based plant health promoting additive; and an agriculturally acceptable excipient, wherein the amount of the melanin synthesis inhibitor fungicide is reduced by at least about 1% to at least about 60% of its fungicidally effective amount.

11. The composition according to claim 10, wherein the agriculturally acceptable excipients include antifreeze agents, dispersants, defoamers, preservatives, thickeners and solvents.

12. A method of reducing the amount of melanin synthesis inhibitor fungicide applied, the method comprising applying to a plant locus, plant part or plant propagation material an agricultural combination comprising: melanin synthesis inhibitor fungicides, the melanin synthesis inhibitor fungicides comprising isobenzofuranones, the isobenzofuranones comprising fthalide; pyrroloquinolinones, the pyrroloquinolinones comprising pyroquilon; triazolothiazoles, the triazolothiazoles comprising tricyclazole; cyclopropanecarboxamides, the cyclopropanecarboxamides comprising carpropamid; carboxamides, the carboxamides comprising diclocymet; propionamides, the propionamides comprising fenoxanil; trifluoroethylcarbamates, the trifluoroethylcarbamates comprising tolprocarb, and mixtures thereof; and at least one silicate-based plant health promoting additive; wherein the amount of melanin synthesis inhibitor fungicide is reduced by about 1% to about 60% of the fungicidally effective amount of said melanin synthesis inhibitor fungicide.

13. The method of claim 12, wherein the melanin synthesis inhibitor fungicide comprises tricyclazole and the silicate-based plant health promoting additive comprises orthosilicic acid.

14. The method of claim 12, wherein the amount of the melanin synthesis inhibitor fungicide is reduced by about 1% to about 55% of its fungicidally effective amount.

15. The method of claim 12, wherein the method comprises reducing the amount of tricyclazole applied, the method comprising applying to a plant locus, plant part, or plant propagation material an agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of melanin synthesis inhibitor fungicide is reduced by about 1% to about 60% of the fungicidally effective amount of tricyclazole.

16. The method of claim 12, wherein the method comprises applying tricyclazole in the range of 50 g ai / ha to 300 g ai / ha and orthosilicic acid in the range of 1 g ai / ha to 70 g ai / ha.

17. The method of claim 12, wherein the method comprises applying tricyclazole in the range of 100 g ai / ha to 300 g ai / ha and orthosilicic acid in the range of 10 g ai / ha to 40 g ai / ha.

18. A method of controlling rice blast, the method of controlling rice blast comprising applying to a plant locus, plant part or plant propagation material an agricultural combination comprising: melanin synthesis inhibitor fungicides, the melanin synthesis inhibitor fungicides comprising isobenzofuranones, the isobenzofuranones comprising fthalide; pyrroloquinolinones, the pyrroloquinolinones comprising pyroquilon; triazolothiazoles, the triazolothiazoles comprising tricyclazole; cyclopropanecarboxamides, the cyclopropanecarboxamides comprising carpropamid; carboxamides, the carboxamides comprising diclocymet; propionamides, the propionamides comprising fenoxanil; trifluoroethylcarbamates, the trifluoroethylcarbamates comprising tolprocarb, and mixtures thereof; and at least one silicate-based plant health promoting additive; wherein the amount of melanin synthesis inhibitor fungicide is reduced by about 1% to about 60% of the fungicidally effective amount of said melanin synthesis inhibitor fungicide.

19. Use of an agricultural combination for reducing the amount of tricyclazole applied to a crop, the agricultural combination comprising tricyclazole and orthosilicic acid, wherein the amount of tricyclazole is reduced by at least about 1% to at least about 60% of the fungicidally effective amount of tricyclazole.

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