Cutting liquid for efficient cutting propagation of hippeastrum bulblet and propagation method

Abstract

The invention provides a cuttage solution for efficient cuttage propagation of hippeastrum bulblet and a propagation method, the formula of the cuttage solution is 2 mg / L 6-BA, 0.8 mg / L NAA and 1 g / L hymexazol, a rapid dipping method and a soaking method used for applying growth regulators in traditional cuttage propagation are abandoned, the cuttage solution is creatively applied in a cuttage medium, and the propagation efficiency is improved. 6-BA, NAA and hymexazol are combined and applied to cutting propagation of hippeastrum rutilum, by means of the specific application method, the hippeastrum rutilum bulblet cutting propagation efficiency is improved, the single-bulb propagation coefficient of hippeastrum rutilum is increased by 7.6%, and the total weight of single-bulb propagation bulblets is increased by 58.8%; meanwhile, the invention provides a propagation method, the step of sterilizing cutting blocks is omitted, the vermiculite mixed fine particle matrix is changed into the vermiculite mixed medium-coarse particle matrix, the later bulblet transplanting step is omitted, the original four-step cutting method is changed into the two-step cutting method, and the labor cost can be saved by 40% or above.

Description

Technical Field

[0001] The invention relates to the technical field of amaryllis planting, and in particular to a cutting liquid for efficient cutting propagation of amaryllis bulblets and a propagation method. Background Art

[0002] Hippeastrum (Hippeastrum spp.) is a perennial bulbous flower of the Amaryllis genus of the Amaryllis family, native to South America. In recent years, due to its high appearance, good meaning, wide variety, easy maintenance, and easy transportation, it has gradually become a trendy flower that is popular all over the world. The supply in my country's New Year market can reach more than one million plants.

[0003] Cutting propagation of Amaryllis is the main mode of propagation of Amaryllis bulblets because of its stable traits, high propagation coefficient, low input cost and easy large-scale production. At present, most of the research related to cutting propagation of Amaryllis focuses on cutting time (Zhang Kezhong et al., 2001; Lv Wentao et al., 2012), cutting method (Yuan Yaling et al., 2008; Kharrazi et al., 2017), substrate preparation (Fang Zhijun, 2023), culture temperature (Zhang et al., 2013), and so on. al., 2013) and other aspects. The steps mainly include bulb cutting, cutting block sterilization, matrix cutting, and small bulb transplanting, a total of 4 steps, the specific steps are: 1) Bulb cutting: cut the mother bulb into multiple cutting blocks; 2) Cutting block sterilization: immerse the cutting block in the sterilization solution for 10 to 30 minutes; 3) Matrix cutting: add water to the vermiculite or vermiculite mixed with fine particles to moisten it, put the cutting block in, and put it together in a plastic bag or plastic frame, and put it in a closed culture environment at 20 to 28 ° C for about 40 to 70 days; 4) Small bulb transplanting: transplant the cuttings with small bulbs into the medium and coarse particle matrix. As can be seen from the above, the existing four-step cutting propagation method has cumbersome steps and high labor costs.

[0004] Growth regulators are believed to promote the cutting propagation process of multiple species. Commonly used methods are the rapid dipping or soaking methods of 10-500 mg / L NAA, IBA, 6-BA, KT and other hormone aqueous solutions. For example, the 300 mg / L NAA rapid dipping method (Liu Xiaofeng, 2009) or the 200 mg / L IBA soaking method for 2 hours (Qi Fengkun et al., 2023) was used in lilies to promote the effect of lily cutting propagation of small bulbs. In daffodils, the 25-50 mg / L 6-BA + 10 mg / L KT soaking method for 24 hours (Zhang Yusen et al., 2024) was used to promote the cutting propagation of small bulbs of daffodils.

[0005] However, in Amaryllis, 50-150 mg / L GA was used. 3 The 5-15 min soaking method of IBA and NAA is not conducive to the propagation of small bulb cuttings (Zhang et al., 2013). 3The treated scales neither produced roots nor bulbs, IBA promoted the growth of basal roots, but the number of bulbs was small, and NAA increased the degree of scale rot. It can be seen that although growth regulators have the effect of regulating plant growth and development, they have not been successfully applied to the cutting propagation process of Amaryllis, resulting in low breeding efficiency.

[0006] Therefore, there is an urgent need for a cutting solution suitable for the cutting propagation of Amaryllis, which can not only improve the propagation efficiency of Amaryllis scales, but also optimize the propagation steps. Summary of the invention

[0007] Purpose of the invention: The purpose of the present invention is to address the deficiencies in the prior art and to provide a cutting fluid and a propagation method for efficient cutting propagation of Amaryllis bulblets. By adding a special cutting fluid during the propagation process, the single-bulb propagation coefficient of Amaryllis and the total weight of single-bulb propagation bulblets are improved, and the traditional four-step cutting propagation method is optimized into a two-step method, thereby improving the propagation efficiency.

[0008] Technical solution: The cutting solution for efficient cutting propagation of amaryllis bulblets described in the present invention comprises the following components: 6-benzylaminopurine (6-BA), naphthaleneacetic acid (NAA) and oxadiazon.

[0009] Furthermore, the formula of the cutting solution is 2mg / L 6-BA+0.8mg / L NAA+1g / L oxadiazine.

[0010] IBA (indolebutyric acid) and NAA (naphthaleneacetic acid) are plant growth regulators that are commonly used to promote rooting and germination in cutting propagation. However, for the cutting propagation of Amaryllis bulblets, these growth regulators may not be applicable or suitable for use alone.

[0011] The invention also provides a high-efficiency cutting propagation method for amaryllis bulblets, and the cutting liquid mentioned above is used in the propagation process.

[0012] Further, the following steps are included:

[0013] Step 1: Bulb cutting: Clean the mother bulb of Amaryllis, remove the dry scales on the outer layer, and cut off 1 / 3 of the top tissue and the bottom root system. Then, cut the bulb into several cuttings and spread them out to dry until there is no obvious water stain on the cut.

[0014] Step 2, matrix cutting: prepare a seedling tray, spread a 6.5 cm thick mixed matrix, place the cutting block flat on the mixed matrix, and then cover it with a 4 cm thick mixed matrix of the same kind, and then pour the cutting solution described in any one of claims 1 to 2 until the water content reaches 60% to 80%, then place the seedling frame on the greenhouse wire mesh seedling bed, the bed surface of the seedling bed is ≥50 cm from the ground, and the seedling frame is covered with a silver-black plastic film with a thickness of 1.5 silks, the silver side of the silver-black plastic film faces up, the greenhouse temperature is controlled within the range of 20 to 28 ° C, the film is uncovered after 40 days, and then the seedlings are cultivated for another 30 days.

[0015] Furthermore, the circumference of the mother bulb of Amaryllis must be ≥12cm and must be healthy and disease-free.

[0016] Furthermore, the cutting method in step 1 is: cut the bulb into 8 to 16 parts along the center according to the size of the bulb, and then use a sharp knife to divide it into 1 to several cutting blocks at the bulb plate according to the size and number of layers of the bulb block, where 1 is not divided, and the cutting block must be ensured to have a bulb plate, the number of scale layers on the bulb plate is ≥2, and the weight of the cutting block is ≥2g.

[0017] Furthermore, the mixed matrix formula in step 2 is a peat soil and vermiculite volume ratio of 4:1, the fiber length of the peat soil ranges from 5 to 20 mm, and the particle length of the vermiculite ranges from 3 to 6 mm.

[0018] Furthermore, during the 30-day cultivation process after the film is removed in step 2, if the substrate humidity drops below 15%, the sterilizing liquid is irrigated once until the moisture content reaches 60% to 80%, wherein the sterilizing liquid is 1 g / L of oxadipate.

[0019] Furthermore, in step 2, the size of the seedling tray is 46cm*46cm*10.5cm, 16 cutting blocks are placed flat in each seedling tray, and the spacing between rows and plants is 11cm.

[0020] In the tissue culture study of Amaryllis, different concentration combinations of 6-BA and NAA had a significant effect on the adventitious bud production of Amaryllis. Studies have shown that adding 1 mg / L of 6-BA and 2 mg / L of NAA to MS medium is the best combination to induce the production of adventitious buds in bulbs. However, tissue culture is a plant propagation technique performed under sterile laboratory conditions, which usually involves placing a small part of the plant tissue (such as the stem tip, leaf or root tip) on a culture medium containing specific nutrients and hormones. Under these conditions, these tissues undergo a process called "callus" formation, which is an undifferentiated cell mass that can be further induced to differentiate into plant organs such as roots and buds. Inducing adventitious buds through the callus pathway means that these undifferentiated cells can be induced to differentiate into any type of plant organs, including adventitious buds (i.e., buds that are not produced by the normal growth pattern of the plant. The cutting propagation method used in the present invention is a traditional plant propagation method in a fungal environment, which involves inserting a part of a plant (usually a branch) into soil or other media to allow it to take root and grow into a new plant. During the cutting process, the regeneration of new buds usually occurs directly from the cutting branches without the formation of callus tissue. The direct regeneration bud pathway means that new buds grow directly from the cutting plant part, rather than through the intermediate stage of callus tissue.

[0021] In addition, in the actual practice of cutting propagation of Amaryllis, sterilized bulb cuttings are usually used, and growth hormones are not used during the cutting process. For example, after soaking the bulb cuttings in a carbendazim solution, dry them and insert them into the soil, keep the soil moist for 40 to 70 days, and small bulbs can be produced. This method does not involve the use of IBA or NAA. At the same time, in some cases, growth regulators such as NAA and IBA have a negative impact on the propagation of small bulbs of Amaryllis when used alone, such as an increase in the rot rate of cuttings or a decrease in the number of small bulbs. Therefore, in order to avoid these side effects during the cutting propagation of Amaryllis, growth regulators are usually not used.

[0022] From the above, it can be seen that there are essential differences between tissue culture and cutting propagation in the mechanism of inducing new bud growth. In the prior art, hormone induction is rarely used in the cutting propagation of Amaryllis bulblets.

[0023] Beneficial effects: Compared with the prior art, the advantages of the present invention are:

[0024] (1) The present invention uses a high-efficiency cutting liquid containing a bactericidal substance to directly moisten the substrate, thereby eliminating the step of sterilizing the cutting block, and changes the vermiculite mixed fine particle substrate to a vermiculite mixed medium-coarse particle substrate, thereby eliminating the step of transplanting the small bulbs in the later stage, and changing the original four-step cutting method to a two-step cutting method, thereby saving more than 40% of the labor cost;

[0025] (2) A high-efficiency cutting liquid containing a plant growth regulator developed by the present invention can increase the single-bulb propagation coefficient of Amaryllis by 7.6% and the total weight of single-bulb propagation bulblets by 58.8%;

[0026] (3) Hormone regulation is widely used in tissue culture propagation under sterile environment, but the application of hormones is an artificial stress. If it is used in the process of cutting propagation under bacterial environment, high concentration can easily lead to scale rot. At the same time, the previous methods of applying hormones to cuttings are all short-term high-concentration rapid dipping or soaking of cuttings, which can easily lead to an increase in the rot rate of cuttings. The present invention creatively adopts a low-concentration irrigation method, which is easy to operate and has a low rot rate.

[0027] (4) The present invention breaks the previous technical prejudice that "NAA is not conducive to the reproduction of small bulbs of Amaryllis, and the application of NAA increases the degree of scale rot". It creatively applies 6-BA, NAA and oxazolidinone in combination for the cutting propagation of Amaryllis. Through the unique application method, the cutting propagation efficiency of small bulbs of Amaryllis is improved. DETAILED DESCRIPTION

[0028] The technical solution of the present invention is described in detail below, but the protection scope of the present invention is not limited to the embodiments.

[0029] Example 1

[0030] The present embodiment provides a cutting solution for efficient cutting propagation of amaryllis bulblets, wherein the cutting solution formula is 2 mg / L 6-BA+0.8 mg / L NAA+1 g / L oxadipate.

[0031] The preparation method is as follows: add 2 mg 6-BA, 0.8 mg NAA and 1 g oxadipate into 1 L aqueous solution.

[0032] Example 2

[0033] The variety of Amaryllis used in this example is the 'Double Delight' bulb (English name: Double Delight), which is a self-propagated bulb of the Shanghai Academy of Agricultural Sciences, with a diameter of 20 to 22 cm, healthy and disease-free. During the cutting propagation process, it was treated with cutting liquid containing different concentrations of growth regulators, 8 bulbs were treated each time, and the propagation effect was counted at 70 days. The specific steps are as follows:

[0034] 1) Bulb cutting: Clean the mother bulb of Amaryllis, remove the dry scales on the outer layer, cut off 1 / 3 of the top tissue and the bottom root system, then cut the bulb into 16 cutting pieces, spread them out to dry until there is no obvious water stain on the cut. The cutting method is to cut longitudinally along the center.

[0035] 2) Matrix cuttings: prepare a seedling tray (46cm*46cm*10.5cm), spread a 6.5cm thick mixed matrix (the volume ratio of peat soil (fiber length 5-20mm) and vermiculite (particle length 3-6mm) is 4:1), place cutting blocks on it (16 pieces are placed in each seedling tray, and the spacing between rows and plants is 11cm), and then cover it with a 4cm thick mixed matrix of the same kind, and then pour cutting liquid containing different concentrations of growth regulators (0-4mg / L 6-BA+0-0.8mg / L NAA+1g / L oxadipate) to a moisture content of 60%-80%, place the seedling frame on the greenhouse wire mesh seedling bed (the bed surface is ≥50cm from the ground), cover it with a 1.5-wire thick silver-black plastic film (silver side facing up), control the greenhouse temperature at 21-25°C, remove the film after 40 days, and then cultivate for another 30 days before data statistics. During this period, when the substrate humidity drops below 15%, 1g / L of oxadipate fungicide solution is irrigated once until the moisture content reaches 60% to 80% oxadipate.

[0036] The reproduction effect was statistically analyzed, and the results are shown in Table 1: the single-bulb reproduction coefficient of the control group without the addition of growth regulators was 19.67, and the total weight of the single-bulb propagated bulblets was 25.2 g; while the single-bulb reproduction coefficient of the experimental group added with 2 mg / L 6-BA and 0.8 mg / L NAA was 21.17, an increase of 7.6% compared with the control group, and the total weight of the single-bulb propagated bulblets was 40.02 g, an increase of 58.8% compared with the control group.

[0037] Table 1 Statistics of reproductive effects under different growth regulator concentrations

[0038]

[0039] Example 3

[0040] The bulbs of the Amaryllis variety used in this example are 'Double Delight' bulbs, which are self-propagated bulbs of Shanghai Academy of Agricultural Sciences, with a diameter of 20 to 22 cm and are healthy and disease-free. Cutting propagation tests were conducted on them with different cutting numbers, with 8 bulbs per treatment, and the propagation effect was counted at 70 days. The specific steps are as follows:

[0041] 1) Bulb cutting: Clean the mother bulbs of Amaryllis, remove the dry scales on the outer layer, cut off 1 / 3 of the top tissue and the bottom root system, then cut the bulbs into 8, 16, 24, and 32 cutting blocks, spread them out to dry until there is no obvious water stain on the cut. The 8-point and 16-point cutting methods are both longitudinally cut along the center, while the 24-point cutting method is 12-point longitudinally cut along the center, and then use a sharp knife to split it into 2 cutting blocks at the bulb plate. The 32-point bulb block is split into 2 cutting blocks at the bulb plate after being 16-pointed. Each cutting block comes with a bulb plate, the number of scale layers on it is ≥2, and the weight of the cutting block is ≥2g.

[0042] 2) Matrix cuttings: prepare a seedling tray (46cm*46cm*10.5cm), spread a 6.5cm thick mixed matrix (peat soil (5-20mm): vermiculite (3-6mm) = 4:1), place cutting blocks on it (16 pieces per seedling tray, with a row spacing of 11cm), and then cover it with a 4cm thick mixed matrix of the same kind, and then pour in a special high-efficiency cutting liquid (2mg / L 6-BA+0.8mg / LNAA+1g / L oxadipate) to a moisture content of 60%-80%, place the seedling frame on the greenhouse wire mesh seedling bed (the bed surface is ≥50cm from the ground), cover it with a 1.5-wire thick silver-black plastic film (silver side facing up), control the greenhouse temperature at 21-25°C, remove the film after 40 days, and then cultivate for another 30 days before data statistics. During this period, when the substrate humidity drops below 15%, 1g / L of carbendazim fungicide solution is irrigated once until the moisture content reaches 60% to 80%.

[0043] The results of the statistical analysis of the propagation effect are shown in Table 2. After the application of the high-efficiency cutting liquid containing bactericidal substances developed by the present invention, the rot rate is basically 0% under the premise that the weight of the cutting block is ≥3g, and the rot rate can be basically controlled within 3% under the premise that the weight of the cutting block is ≥2g. In addition, as the number of cuttings increases, the single bulb propagation coefficient gradually increases, but the single weight of the small bulb also gradually decreases. Therefore, in actual production, an appropriate bulb cutting strategy can be selected according to production needs.

[0044] Table 2 Statistics of reproduction effects under different cutting methods

[0045]

[0046] Comparative Example 1

[0047] The variety of Amaryllis used in this example is the 'Double Delight' bulb (English name: Double Delight), which is a self-propagated bulb of the Shanghai Academy of Agricultural Sciences, with a diameter of 20 to 22 cm, healthy and disease-free. In the process of cutting propagation, the cutting block was soaked in a fungicide for 10 minutes and the high-concentration growth regulator was soaked for 10 minutes. Each treatment was 8 bulbs, and the propagation effect was counted at 70 days. The specific steps are as follows:

[0048] 1) Bulb cutting: Clean the mother bulb of Amaryllis, remove the dry scales on the outer layer, cut off 1 / 3 of the top tissue and the bottom root system, then cut the bulb into 16 cutting pieces, spread them out to dry until there is no obvious water stain on the cut. The cutting method is to cut longitudinally along the center.

[0049] 2) Sterilization of cuttings: Immerse the cuttings in a 500-fold diluted carbendazim sterilization solution for 10 minutes.

[0050] 3) Soaking in high concentration growth regulators: After taking out the cuttings from the sterilization solution, immerse them in an aqueous solution containing different concentrations of growth regulators (0-80 mg / L 6-BA + 0-80 mg / L NAA) for 10 minutes, and then take them out for use.

[0051] 4) Matrix cuttings: Prepare a seedling tray (46cm*46cm*10.5cm), spread a 6.5cm thick mixed matrix (the volume ratio of peat soil (fiber length 5-20mm) and vermiculite (particle length 3-6mm) is 4:1), and place the cutting blocks treated in the above steps on it (16 pieces are placed in each seedling tray, and the spacing between plants and rows is 11cm), and then cover it with a 4cm thick mixed matrix of the same kind, and then water it to a moisture content of 60% to 80%. Place the seedling frame on the greenhouse wire mesh seedling bed (the bed surface is ≥50cm from the ground), and cover it with a silver-black plastic film with a thickness of 1.5 silk (silver side facing up), and control the greenhouse temperature at 21-25℃. After 40 days, remove the film, and then cultivate for another 30 days to perform data statistics. During this period, when the matrix humidity drops below 15%, water it once until the moisture content reaches 60% to 80%.

[0052] The results of the statistical analysis of the propagation effects showed that when NAA was applied alone, within the concentration range of 20-80 mg / L, the decay rate increased with the increase of concentration, and the single weight of the bulblets, the single bulb reproduction coefficient and the total weight of the bulblets propagated by the single bulblet decreased sharply with the increase of concentration, indicating that the application of 20-80 mg / L NAA was not conducive to the propagation of the bulblets; when 6-BA was applied alone, within the concentration range of 20-80 mg / L, the decay rate and the single weight of the bulblets first decreased and then increased with the increase of concentration, and the reproduction coefficient showed a trend of first increasing and then decreasing, but the total weight of the bulblets propagated by the single bulblet did not change much, indicating that the application of 20-80 mg / L 6-BA can slightly adjust the unit weight and reproduction coefficient of small bulbs, but the overall gain is not large; when NAA (20-80 mg / L) and 6-BA (20-80 mg / L) mixed hormones are applied, the rot rate is higher than that of the control group without hormone application, and the single bulb reproduction coefficient and the total weight of small bulbs propagated by single bulbs are lower than those of the control group, indicating that an inappropriate concentration of mixed hormones is also not conducive to the reproduction of small bulbs.

[0053] Table 3 Statistics of reproductive effects under different growth regulator concentrations

[0055]

[0056] As described above, although the present invention has been shown and described with reference to specific preferred embodiments, it should not be construed as limiting the present invention itself. Various changes in form and details may be made without departing from the spirit and scope of the present invention as defined in the appended claims.

Claims

1. A cutting solution for efficient cutting propagation of Amaryllis bulblets, characterized in that Contains the following ingredients: 6-BA, NAA and oxadiazine.

2. The cutting liquid for efficient cutting propagation of Amaryllis bulblets according to claim 1, characterized in that: The cutting solution formula is 2mg / L6-BA+0.8mg / L NAA+1g / L oxadipate.

3. A highly efficient cutting propagation method for Amaryllis bulblets, characterized in that: The cutting solution as claimed in any one of claims 1 to 2 is used during the propagation process.

4. A method for efficient cutting propagation of Amaryllis bulblets according to claim 3, characterized in that The steps include: Step 1: Bulb cutting: Clean the mother bulb of Amaryllis, remove the dry scales on the outer layer, and cut off 1 / 3 of the top tissue and the bottom root system. Then, cut the bulb into several cuttings and spread them out to dry until there is no obvious water stain on the cut. Step 2, matrix cutting: prepare a seedling tray, spread a 6.5 cm thick mixed matrix, place the cutting block flat on the mixed matrix, and then cover it with a 4 cm thick mixed matrix of the same kind, and then pour the cutting solution described in any one of claims 1 to 2 until the water content reaches 60% to 80%, then place the seedling frame on the greenhouse wire mesh seedling bed, the bed surface of the seedling bed is ≥50 cm from the ground, and the seedling frame is covered with a silver-black plastic film with a thickness of 1.5 silks, the silver side of the silver-black plastic film faces up, the greenhouse temperature is controlled within the range of 20 to 28 ° C, the film is uncovered after 40 days, and then the seedlings are cultivated for another 30 days.

5. A highly efficient cutting propagation method for Amaryllis bulblets according to claim 4, characterized in that: The circumference of the mother bulb of Amaryllis must be ≥12 cm and it must be healthy and free of diseases.

6. A highly efficient cutting propagation method for Amaryllis bulblets according to claim 4, characterized in that: The cutting method in step 1 is: cut the bulb into 8 to 16 parts along the center according to the size of the bulb, and then use a sharp knife to divide it into 1 to several cutting blocks at the bulb plate according to the size and number of layers of the bulb, where 1 is not divided. The cutting block must be ensured to have a bulb plate, the number of scale layers on the bulb plate is ≥2, and the weight of the cutting block is ≥2g.

7. A highly efficient cutting propagation method for Amaryllis bulblets according to claim 4, characterized in that: The mixed matrix formula in step 2 is a volume ratio of peat soil to vermiculite of 4:1, the fiber length of the peat soil ranges from 5 to 20 mm, and the particle length of the vermiculite ranges from 3 to 6 mm.

8. A highly efficient cutting propagation method for Amaryllis bulblets according to claim 4, characterized in that: During the 30-day cultivation process after the film is uncovered in step 2, when the substrate humidity drops below 15%, the substrate is irrigated with a sterilizing solution once until the moisture content reaches 60% to 80%, and the sterilizing solution is 1 g / L of oxadipate.

9. A highly efficient cutting propagation method for Amaryllis bulblets according to claim 4, characterized in that: In step 2, the size of the seedling tray is 46cm*46cm*10.5cm, and 16 cutting blocks are placed flat in each seedling tray, with a row spacing of 11cm.

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