Plant stress-resistant agent and application thereof

Abstract

The invention belongs to the technical field of agriculture, and particularly relates to a plant stress-resistant agent and application thereof, and the plant stress-resistant agent comprises silicic acid, molybdic acid and a biological enzyme preparation; the composition also comprises a pesticide pharmacologically acceptable carrier and a preparation auxiliary agent. The stress-resistant agent disclosed by the invention is applied to improvement of crop stress resistance, has the characteristics of quick action, remarkable effect and no toxic or side effect, has a remarkable improvement effect on the crop stress resistance, and can effectively promote the crop stress resistance in drought and low-temperature stress after being applied.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture, and in particular relates to a plant stress resistance agent and an application thereof for improving plant stress resistance. Background Art

[0002] In the process of agricultural planting and production practice, changes in natural geographical conditions and climatic conditions can cause various adverse effects on the cultivated crops. The influence of adverse factors such as low temperature, drought, strong wind, etc. will affect the normal growth and development of crops, causing damage or even death of crops. The adaptability and resistance of crops to adverse environments are called stress resistance or stress resistance. Crop stress resistance is the ability of crops to resist adverse external factors, such as drought resistance, salinity resistance, waterlogging resistance, wind resistance, frost resistance, disease and insect pest resistance, etc.

[0003] When silicic acid and molybdic acid are applied to the same crop at the same time, they work simultaneously, eliminating pesticide damage and promoting crop growth. However, the cost of using silicic acid and molybdic acid at the same time is high, and excessive use can easily lead to a series of environmental problems. Research has found that mixing silicic acid, molybdic acid and biological enzyme preparations can not only reduce the dosage, but also promote the development of plant roots at a certain mass ratio, which can significantly improve the stress resistance effect. Summary of the invention

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art and provide a plant stress-resistant agent and its application. The plant stress-resistant agent is prepared by mixing silicic acid, molybdic acid and a biological enzyme preparation, which can effectively improve the resistance of crops to adverse external factors.

[0005] The purpose of the present invention is to provide a plant stress-resistant agent, which comprises silicic acid, molybdic acid, and a biological enzyme preparation; and also comprises a carrier and a formulation adjuvant that are acceptable in pesticides;

[0006] Furthermore, the mass ratio of silicic acid to molybdic acid is 1:10 to 10:1;

[0007] Furthermore, the mass ratio of silicic acid to molybdic acid is 5:13 and 3:5;

[0008] Furthermore, the silicic acid and molybdic acid together account for 0.5% to 20% of the stress-resistant agent by weight;

[0009] Furthermore, the silicic acid and molybdic acid together account for 1% to 5% of the stress-resistant agent by weight;

[0010] Furthermore, the biological enzyme is at least one or more of superoxide dismutase, protease, amylase, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase;

[0011] Furthermore, the mass ratio of superoxide dismutase, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase is 6:3:3:3:2:2:2:2:2:2;

[0012] Furthermore, the mass ratio of superoxide dismutase, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase is 3:2:2:2:1:1:1:1:1:1;

[0013] Furthermore, the mass ratio of superoxide dismutase, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase is 6:3:3:3:3:3:1:1:1:1;

[0014] Furthermore, the stress-resistant agent comprises 0.5% to 10% of silicic acid, 0.5% to 10% of molybdic acid, 1% to 5% of biological enzyme preparation, 3% to 15% of emulsifier, 1% to 30% of stabilizer, and deionized water makes up the balance;

[0015] Furthermore, the stress-resistant agent comprises 1% to 5% of silicic acid, 1% to 5% of molybdic acid, 1% to 5% of biological enzyme preparation, 3% to 15% of emulsifier, 5% to 15% of stabilizer, and deionized water makes up the balance;

[0016] Furthermore, the formulation auxiliary agent is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier;

[0017] Further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, saponin powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0018] Further, the dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0019] Further, the emulsifier is selected from one or more of calcium dodecylbenzene sulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0020] Furthermore, the thickener is selected from one or more of xanthan gum, organic bentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0021] Furthermore, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0022] Furthermore, the antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0023] Furthermore, the defoaming agent is selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0024] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0025] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0026] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0027] Furthermore, the synergist is selected from synergist phosphine and piperonyl ether; and / or

[0028] Furthermore, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives.

[0029] A stress-resistant agent is used to improve the drought resistance and frost resistance of plants;

[0030] Furthermore, the plant is a food crop, cash crop, industrial raw material crop, feed crop or medicinal crop;

[0031] Furthermore, the plants are citrus, eggplant, pepper, tomato, cucumber, and strawberry;

[0032] Furthermore, the stress resistance agent is applied to the crops to be controlled or the area where the crops grow in an effective dose.

[0033] The plant growth regulator and stress-resistant agent of the present invention has the following advantages:

[0034] The mixed use of silicic acid, molybdic acid and biological enzyme preparation of the present invention has the characteristics of rapid action on the target, significant effect and no toxic side effects, and has a significant effect on improving the stress resistance of crops, can reduce the amount of pesticides used, and can effectively promote the drought resistance and frost resistance of crops after application. DETAILED DESCRIPTION

[0035] In order to make the present application easier to understand, the present application will be further described in detail below in conjunction with the examples, which are merely illustrative and are not intended to limit the scope of application of the present application. The raw materials or components used in the present application can be obtained through commercial routes or conventional methods unless otherwise specified.

[0036] Preparation Example

[0037] In order to better illustrate the present invention, the content of the present invention is further described below in conjunction with the embodiments. The parts in all the embodiments are parts by weight.

[0038] Preparation Example 1:

[0039] 1.5 parts of silicic acid, 2.5 parts of molybdic acid and 8 parts of ethylene glycol are added into deionized water, and 10 parts of xylene and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) are added while stirring, and stirred for half an hour to obtain a mixed solution; 3 parts of biological enzyme preparations (0.8% superoxide dismutase, 0.4% protease, 0.4% urease, 0.4% cellulase, 0.3% lipase, 0.3% phytase, 0.1% pectinase, 0.1% xylanase, 0.1% β-glucanase and 0.1% β-mannanase) are added into the mixed solution and stirred, and then the solution is transferred into a reactor, reacted at 25°C for 1 hour, and a plant stress resistance agent is obtained after the reaction is completed.

[0040] Preparation Example 2:

[0041] 1.5 parts of silicic acid, 2.5 parts of molybdic acid and 8 parts of ethylene glycol are added into deionized water, and 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) are added while stirring, and after stirring for half an hour, a mixed solution is obtained; 2.6 parts of biological enzyme preparation (0.8% superoxide dismutase, 0.6% protease, 0.6% urease and 0.6% cellulase) are added into the mixed solution, stirred, and then transferred into a reactor, reacted at 25°C for 1 hour, and a plant stress resistance agent is obtained after the reaction is completed.

[0042] Preparation Example 3:

[0043] 1 part of silicic acid, 2.6 parts of molybdic acid and 8 parts of ethylene glycol are added into deionized water, and 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) are added while stirring, and stirred for half an hour to obtain a mixed solution; 2.7 parts of biological enzyme preparation (0.6% superoxide dismutase, 0.3% protease, 0.3% urease, 0.3% cellulase, 0.2% lipase, 0.2% phytase, 0.2% pectinase, 0.2% xylanase, 0.2% beta glucanase and 0.2% beta mannanase) are added into the mixed solution and stirred, and then the solution is transferred into a reactor, reacted at 25°C for 1 hour, and a plant stress resistance agent is obtained after the reaction is completed.

[0044] Preparation Example 4:

[0045] 1 part of silicic acid, 2.6 parts of molybdic acid and 8 parts of ethylene glycol are added into deionized water, and 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) are added while stirring, and stirred for half an hour to obtain a mixed solution; 3 parts of biological enzyme preparations (0.6% superoxide dismutase, 0.4% protease, 0.4% urease, 0.4% cellulase, 0.2% lipase, 0.2% phytase, 0.2% pectinase, 0.2% xylanase, 0.2% beta glucanase and 0.2% beta mannanase) are added into the mixed solution and stirred, and then the solution is transferred into a reactor, reacted at 25°C for 1 hour, and a plant stress resistance agent is obtained after the reaction is completed.

[0046] Preparation Example 5:

[0047] 1 part of silicic acid, 2.6 parts of molybdic acid and 8 parts of ethylene glycol are added into deionized water, and 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) are added while stirring, and stirred for half an hour to obtain a mixed solution; 2.5 parts of biological enzyme preparation (0.6% superoxide dismutase, 0.3% protease, 0.3% urease, 0.3% cellulase, 0.3% lipase, 0.3% phytase, 0.1% pectinase, 0.1% xylanase, 0.1% β-glucanase and 0.1% β-mannanase) are added into the mixed solution and stirred, and then the solution is transferred into a reactor, reacted at 25°C for 1 hour, and a plant stress resistance agent is obtained after the reaction is completed.

[0048] Comparative Example 1:

[0049] 1.5 parts of silicic acid, 2.5 parts of molybdic acid and 8 parts of ethylene glycol were added into deionized water, and 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) were added while stirring. After stirring for half an hour, a mixed solution was obtained. The solution was then transferred into a reactor and reacted at 25°C for 1 hour. After the reaction, a plant stress resistance agent was obtained.

[0050] Comparative Example 2:

[0051] Add 1 part of silicic acid, 2.6 parts of molybdic acid and 8 parts of ethylene glycol into deionized water, add 10 parts of epoxidized vegetable oil and 5 parts of styrylphenol polyoxyethylene ether (Nongru 600#) while stirring, and stir for half an hour to obtain a mixed solution; stir, then transfer into a reactor, react at 25°C for 1 hour, and obtain a plant stress resistance agent after the reaction is completed.

[0052] Example 1

[0053] Effects of drought stress on citrus growth and drought resistance

[0054] Test crops: Citrus seedlings (1.5 months old), transplanted into ceramic pots, one plant per pot, 5 plants per treatment, each treatment was repeated 4 times, totaling 20 plants.

[0055] Chemical treatment: Carry out drought stress test when citrus seedlings grow well. Before drought stress treatment, apply the above stress resistance agents once. Use conventional spraying method (30L of water per mu) to spray the whole citrus plants with the test agent. The amount of application should be such that the leaves are evenly covered with the drug and a little drop of liquid falls.

[0056] The treatments are shown in the following table:

[0057] Table 1 Experimental treatment settings

[0058] deal with Specific medicine Double liquid Process 1 Preparation Example 1 2000 Process 2 Preparation Example 2 2000 Process 3 Preparation Example 3 2000 Process 4 Preparation Example 4 2000 Process 5 Preparation Example 5 2000 Process 6 Comparative Example 1 2000 Process 7 Comparative Example 2 2000 Process 8 CK Spray equal amount of water

[0059] The drought stress time was 30 days (the volumetric water content of the control soil was maintained at 9% to 13%);

[0060] Sampling: Relevant indicators were measured on the 30th day of drought stress.

[0061] Determination of seedling growth index:

[0062] Determination of plant height and stem diameter: Use a tape measure to measure the length from the substrate surface to the top of the plant, and use a digital vernier caliper to measure the diameter at a height of 2 cm from the substrate.

[0063] Longest lateral root length: Use a ruler to measure the length from the base of the root to the longest part of the root.

[0064] Test results:

[0065] The results in Table 2 show that spraying stress-resistant agents can effectively increase the plant height, stem thickness and longest lateral root length of citrus seedlings, among which the plant height and longest lateral root length of citrus seedlings treated with Preparation Examples 3 to 5 are significantly greater than those of Preparation Example 1, Preparation Example 2 and the control example. It can be seen that the application of the stress-resistant agents of Preparation Examples 3 to 5 can significantly promote the regeneration and elongation of the root system of citrus seedlings, expand the root volume, thereby increasing the absorption and transportation capacity of the root system and improving drought resistance.

[0066] Table 2 Effects of various treatments on the growth and development of citrus seedlings

[0067] Specific medicine Double liquid Plant height (cm) Stem diameter (cm) The longest lateral root length (cm) Preparation Example 1 2000 39.06b 1.36a 18.19b Preparation Example 2 2000 38.25b 1.38a 17.76b Preparation Example 3 2000 40.93a 1.48a 21.48a Preparation Example 4 2000 41.48a 1.49a 22.35a Preparation Example 5 2000 42.09a 1.48a 21.38a Comparative Example 1 2000 36.30c 1.42a 18.44b Comparative Example 2 2000 35.78c 1.40a 17.67b CK Shimizu 30.74d 1.39a 14.91c

[0068] Note: The data in the table are the average values ​​of repeated measurements. Lowercase letters represent the differences of various indicators in different treatments at the 0.05 level.

[0069] Example 2

[0070] Test location: The test was conducted in the Shouguang solar greenhouse in Weifang;

[0071] Experimental materials: Cucumber (Cucumis sativus L.);

[0072] Temperature: The lowest temperature in the solar greenhouse during the entire test period was 3°C (more than 7 days);

[0073] Experimental design: A total of 6 treatments were set up in the experiment, 5 chemical treatments and 1 blank control (the same amount of clean water was sprayed at the same time as the above chemical treatments).

[0074] Experimental treatment: Each treatment was repeated 4 times, with an area of ​​30 cm 2 The cucumbers in each treatment were managed according to conventional fertilization and other agricultural treatments.

[0075] Table 3 Experimental treatment settings

[0076] deal with Specific medicine Double liquid Process 1 Preparation Example 3 1500 Process 2 Preparation Example 4 1500 Process 3 Preparation Example 5 1500 Process 4 Comparative Example 1 1500 Process 5 Comparative Example 2 1500 Process 6 Blank control Spray equal amount of water

[0077] Chemical treatment: The experiment started spraying the chemicals for each treatment 30 days after cucumber planting, and the second spraying was carried out after an interval of 15 days, for a total of 2 sprayings, with a water consumption of 30L per mu.

[0078] Determination index and determination method: 10 cucumber plants were investigated in each repetition.

[0079] 1) Plant height: The natural height from the substrate surface to the most leaves in the seedling stage was measured with a ruler on October 10.

[0080] 2) Leaf area: Take the second fully expanded functional leaf at the seedling stage and measure the length and width of the central leaflet of the compound leaf with a ruler.

[0081] 3) Stem diameter: Use a vernier caliper to measure the diameter of the base of the cucumber stem at the seedling stage.

[0082] Experimental results: Cucumbers prefer warmth and are not cold-resistant. The suitable temperature for their growth and development is 10-32°C. They are easily affected by low temperature stress during the planting and cultivation process, resulting in reduced yield and quality.

[0083] The test results show (see Table 4) that the application of the stress-resistant agents of Preparation Example 3, Preparation Example 4, and Preparation Example 5 can effectively reduce the impact of low temperature stress on cucumbers, significantly increase the plant height, stem thickness, and leaf area of ​​cucumbers, significantly improve the comprehensive resistance of cucumbers to low temperature stress, and improve plant photosynthesis and nutrient transport to resist low temperature stress, thereby ensuring normal growth and development.

[0084] Table 4 Effects of various treatments on cucumber growth under low temperature stress

[0085] deal with Plant height (cm) Stem diameter (mm) <![CDATA[Leaf area (cm 2 )]]> Process 1 17.19a 14.91a 80.23a Process 2 17.35a 15.37a 88.70a Process 3 16.85a 15.45b 88.69c Process 4 15.91b 12.41b 67.59b Process 5 15.51b 11.03c 68.41b Process 6 13.16c 10.22c 66.75b

[0086] Note: The data in the table are the average values ​​of repeated measurements. Lowercase letters represent the differences of various indicators in different treatments at the 0.05 level.

[0087] The above content is a further detailed description of the present invention in combination with specific implementation methods. It cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as belonging to the scope of protection determined by the claims submitted for the present invention.

Claims

1. A plant stress resistance agent, characterized in that: The stress-resistant agent includes silicic acid, molybdic acid, biological enzyme preparation, and also includes pesticide-permissible carriers and formulation adjuvants.

2. The anti-adverse agent according to claim 1, characterized in that: The mass ratio of silicic acid to molybdic acid is 1:10 to 10:1; Preferably, the mass ratio of silicic acid to molybdic acid is 5:

13.

3. The anti-adverse agent according to claim 1, characterized in that: The silicic acid and molybdic acid together account for 0.5% to 20% of the stress-resistant agent by weight; Preferably, the silicic acid and molybdic acid together account for 1% to 5% by weight of the stress-resistant agent.

4. The anti-adverse agent according to claim 1, characterized in that: The biological enzyme is at least one or more of superoxide dismutase, protease, amylase, urease, cellulase, lipase, phytase, pectinase, xylanase, beta glucanase and beta mannanase.

5. The anti-adverse agent according to claim 4, characterized in that: The mass ratios of superoxide dismutase, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase are 6:3:3:3:2:2:2:2:2:2, 3:2:2:2:1:1:1:1:1, and 6:3:3:3:3:3:1:1:1:1; 6. The anti-adverse agent according to claim 1, characterized in that: The formulation auxiliary agent is selected from one or more of a wetting agent, a dispersant, an emulsifier, a thickener, a disintegrant, an antifreeze agent, a defoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

7. Use of the stress-resistant agent according to any one of claims 1 to 6 for improving drought resistance and frost resistance of plants.

8. The use according to claim 7, characterized in that The plants are food crops, cash crops, industrial raw material crops, feed crops or medicinal crops.

9. The use according to claim 8, characterized in that The plants are citrus, eggplant, pepper, tomato, cucumber and strawberry.

10. The anti-adverse agent according to claim 9, characterized in that: The stress-resistant agent is applied to the crops to be controlled or the area where the crops grow at an effective dose.