Composition with cell light damage repairing effect and application thereof

Abstract

The invention discloses a composition with an effect of repairing cell light damage and application of the composition, the composition is composed of small-molecule kinetic energy elements and small-molecule collagen peptide according to the mass ratio of 1: (0.5-10), the small-molecule kinetic energy elements comprise sodium mannose phosphate, and the small-molecule collagen peptide is giant salamander hydrolyzed collagen. The composition is compounded by taking the giant salamander hydrolyzed collagen as micromolecular collagen peptide and taking a component containing sodium mannose phosphate as micromolecular kinetic energy, and can help to promote growth of a better collagen chain of a support structure and resist oxidation so as to play a role in repairing cell light damage.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a composition having the effect of repairing photodamage of cells and application thereof. Background Art

[0002] Skin photodamage is damage caused by long-term exposure to sunlight. Exposure to ultraviolet rays can cause significant changes in skin collagen tissue, and produce a large amount of reactive oxygen species (ROS), causing cell damage and inflammation. According to wavelength, ultraviolet rays can be divided into UVA, UVB, and UVC. UVC is absorbed and dispersed by the atmospheric ozone layer and cannot reach the earth's surface. UVA has a strong ability to penetrate the skin and can affect the dermis and subcutaneous tissue areas, causing cell damage such as fibroblasts, vascular endothelial cells, and Langerhans cells, causing changes in the dermis structure, leading to sagging and wrinkling of the skin; UVB has higher energy but relatively weaker penetration, mainly affecting skin keratinocytes and melanocytes, which can thicken the stratum corneum, cause epidermal hyperplasia, and intensify inflammatory reactions.

[0003] In the prior art, CN11123508A discloses a short bifidobacterium with the effects of repairing ultraviolet damage, relieving inflammation, and preventing skin photoaging. By studying its action principle on the main action proteases such as MMP-1 (interstitial collagenase) and MMP-3 (matrilysin), a short bifidobacterium that can improve the skin photoaging phenomenon caused by long-term repeated ultraviolet irradiation is cultivated, but the short bifidobacterium needs a long time to be cultivated, and the yield is small, which is not conducive to large-scale promotion. CN116158992A discloses a composition with the effect of repairing cell light damage, which achieves the effect of repairing cell light damage by using retinol and ginkgo leaf extract in a specific ratio, and studying factors such as skin light damage and ROS level, mitogen-activated protein kinase (MAPK), and MMP-1 (matrix metalloproteinase-1) expression. Although the ginkgo leaf extract has a fresh grassy smell, similar to the mint fragrance, its smell is light and cannot cover the special smell and bitterness of retinol, which affects its use. Summary of the invention

[0004] In order to overcome the deficiencies of the prior art, one of the objectives of the present invention is to provide a composition having the effect of repairing photodamage to cells, so as to solve the above-mentioned traditional technical problems.

[0005] The second object of the present invention is to provide a composition having the effect of repairing photodamage to cells for use in the preparation of cosmetics, wherein the cosmetics include essence milk, essence lotion, emulsion, cream and mask.

[0006] One of the purposes of the present invention is achieved by the following technical solution:

[0007] A composition with the effect of repairing cell light damage is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic energy includes sodium mannose phosphate, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0008] Among them, the mass ratio of small molecule kinetic energy element and small molecule collagen peptide is 1: (0.5-10) such as 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, 1:10

[0009] In the present invention, giant salamander hydrolyzed collagen is selected as a small molecule collagen peptide. Giant salamander hydrolyzed collagen contains 17 kinds of amino acids. Its collagen peptide chain amino acid sequence is mainly repeated with Gly-X-Y. It has a unique cyclic molecular structure and is an essential structure for the composition of collagen chains. It can give collagen better microelasticity and strong tensile strength. It contains 2400m / z small molecule hydrolyzed collagen, which can penetrate into the deep layer of the skin and provide nutrients required for cells and skin. Sodium mannose phosphate is selected as a small molecule kinetic element. The small molecule kinetic element can directly supply energy to the cell power source (mitochondria). Under the blessing of this component, the vitality of the maternal cells of the "collagen manufacturing machine" is greatly improved, with a full energy motive force, and the "super collagen chain" is promoted. Using giant salamander hydrolyzed collagen as a small molecule collagen peptide and sodium mannose phosphate as a small molecule kinetic element compound can help promote the growth of collagen chains with better supporting structures and anti-oxidation, so as to play a role in repairing cell light damage.

[0010] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:(2-4).

[0011] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:2.

[0012] One of the purposes of the present invention is also achieved by the following technical solution:

[0013] A composition with the effect of repairing cell light damage, composed of a small molecule kinetic element and a small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic element comprises sodium mannose phosphate and passion flower extract in a mass ratio of 1: (0.1-0.3), and the small molecule collagen peptide is hydrolyzed collagen of giant salamander;

[0014] Among them, the mass ratio of small molecule kinetic energy and small molecule collagen peptide is 1:(0.5-10) such as 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, and 1:10.

[0015] In the present invention, giant salamander hydrolyzed collagen is selected as small molecule collagen peptide, sodium mannose phosphate and passion flower extract are selected as small molecule kinetic energy element, passion flower extract can effectively delay the process of self skin aging, clean up various harmful residues of human skin cells, and after passion flower extract and sodium mannose phosphate are compounded, its cell repair effect is improved. Using giant salamander hydrolyzed collagen as small molecule collagen peptide, sodium mannose phosphate, passion flower extract as small molecule kinetic energy element compounding, further can help promote the growth of better collagen chains and anti-oxidation of supporting structure, so as to play the role of repairing cell light damage.

[0016] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:(2-4).

[0017] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:2.

[0018] Furthermore, the mass ratio of sodium mannose phosphate to passion flower extract is 1:0.3.

[0019] One of the purposes of the present invention is also achieved by the following technical solution:

[0020] A composition with the effect of repairing cell light damage, composed of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic energy element comprises sodium mannose phosphate and a gentiana macrophylla extract in a mass ratio of 1: (0.05-0.1), and the small molecule collagen peptide is a hydrolyzed collagen of a giant salamander;

[0021] Among them, the mass ratio of small molecule kinetic energy and small molecule collagen peptide is 1:(0.5-10) such as 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, and 1:10.

[0022] In the present invention, hydrolyzed collagen from giant salamander is selected as small molecule collagen peptide, sodium mannose phosphate and qinjiao extract are selected as small molecule kinetic energy element, qinjiao extract contains components that can prevent cell division, and after being combined with sodium mannose phosphate, it can promote the effect of cell repair. The combination of hydrolyzed collagen from giant salamander as small molecule collagen peptide, sodium mannose phosphate and qinjiao extract as small molecule kinetic energy element can help promote the growth of collagen chains with better supporting structure and anti-oxidation, so as to repair cell light damage.

[0023] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:(2-4).

[0024] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:2.

[0025] Furthermore, the mass ratio of sodium mannose phosphate to the Gentiana macrophylla extract is 1:0.1.

[0026] One of the purposes of the present invention is also achieved by the following technical solution:

[0027] A composition with the effect of repairing cell light damage, composed of a small molecule kinetic element and a small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic element comprises sodium mannose phosphate, passion flower extract and gentiana macrophylla extract in a mass ratio of 1: (0.1-0.3): (0.05-0.1), and the small molecule collagen peptide is hydrolyzed collagen of giant salamander;

[0028] Among them, the mass ratio of small molecule kinetic energy and small molecule collagen peptide is 1:(0.5-10) such as 1:0.5, 1:1, 1:1.5, 1:2, 1:2.5, 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8, 1:8.5, 1:9, 1:9.5, and 1:10.

[0029] In the present invention, hydrolyzed collagen of giant salamander is selected as small molecule collagen peptide, sodium mannose phosphate, passion flower extract and qin macrophylla extract are selected as small molecule kinetic energy element, passion flower extract and qin macrophylla extract are matched with sodium mannose phosphate to further promote the cell repair effect of sodium mannose phosphate. The combination of hydrolyzed collagen of giant salamander as small molecule collagen peptide, sodium mannose phosphate, passion flower extract and qin macrophylla extract as small molecule kinetic energy element can help promote the growth of collagen chains with better supporting structure and anti-oxidation, so as to play a role in repairing cell light damage.

[0030] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:(2-4).

[0031] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:2.

[0032] Furthermore, the mass ratio of sodium mannose phosphate, passion flower extract and gentiana macrophylla extract is 1:0.1:0.1. After compounding the gentiana macrophylla extract, the amount of passion flower extract can be increased, and the compounding effect is better.

[0033] One of the purposes of the present invention is also achieved by the following technical solution:

[0034] A composition with the effect of repairing cell light damage, composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10), the small molecule kinetic energy includes sodium mannose phosphate, passion flower extract, gentiana macrophylla extract and snow lotus in a mass ratio of 1: (0.1-0.3): (0.05-0.1): (0.01-0.1), and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0035] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:(2-4).

[0036] Furthermore, the mass ratio of small molecule kinetic energy to small molecule collagen peptide is 1:2.

[0037] Furthermore, the mass ratio of sodium mannose phosphate, passion flower extract, gentiana macrophylla extract: snow lotus is 1:0.1:0.1:0.01.

[0038] Furthermore, the Tianshan Snow Lotus is divided into white Tianshan Snow Lotus and purple Tianshan Snow Lotus, and preferably the Tianshan Snow Lotus is divided into white Tianshan Snow Lotus.

[0039] The second object of the present invention is achieved by adopting the following technical solution:

[0040] An application of the above-mentioned composition with the effect of repairing cell light damage is to use the composition with the effect of repairing cell light damage in the preparation of cosmetics, and the cosmetics include essence milk, essence lotion, emulsion, cream and mask.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The composition of the present invention uses giant salamander hydrolyzed collagen as a small molecule collagen peptide. Giant salamander hydrolyzed collagen contains 17 kinds of amino acids. Its collagen peptide chain amino acid sequence is mainly repeated with Gly—X—Y. It has a unique cyclic molecular structure and is an essential structure for the composition of collagen chains. It can give collagen better microelasticity and stronger tensile strength. It contains 2400m / z small molecule hydrolyzed collagen, which can penetrate into the deep layer of the skin and provide nutrients required by cells and skin. The component containing sodium mannose phosphate is selected as a small molecule kinetic element. The small molecule kinetic element can directly supply energy to the cell power source (mitochondria). Under the blessing of this component, the vitality of the maternal cells of the "collagen manufacturing machine" is greatly improved, with a full energy motive force, and promotes the production of "super collagen chains". The combination of giant salamander hydrolyzed collagen as a small molecule collagen peptide and a component containing sodium mannose phosphate as a small molecule kinetic element can help promote the production of collagen chains with better supporting structures and anti-oxidation, so as to play a role in repairing cell light damage. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 Schematic diagram of the loss of vitality of skin matrix cells;

[0044] Figure 2 This is a schematic diagram of the principle of the combined composition of small molecule kinetic energy and small molecule collagen peptide acting on the skin;

[0045] Figure 3 The figure is a graph of the permeation amount of the composition of Example 3 at different times within 24 hours;

[0046] Figure 4 is a graph of the cumulative permeation of the composition of Example 3 within 24 hours;

[0047] Figure 5 is a transmittance graph of the composition of Example 3 at different times within 24 hours;

[0048] Figure 6 is a graph of the cumulative permeability of the composition of Example 3 within 24 hours;

[0049] Figure 7 The figure shows the effect of different concentrations of samples on the expression of p62, LC3-II and β-actin proteins in HaCaT, where 1 is the normal control group, 2 to 4 are the sample groups of 0.1%, 0.25% and 0.75%, respectively;

[0050] Figure 8 It is a bar graph of the p62 / β-actin ratio;

[0051] Fig. 9 It is a bar graph of LC3-Ⅱ / β-actin ratio. DETAILED DESCRIPTION

[0052] The synergistic mechanism of the small molecule collagen peptide and small molecule kinetic energy element of the present invention is:

[0053] The dermis is a "collagen factory". The energetic "collagen manufacturing machine" maternal cells will continuously produce collagen, elastin, and hyaluronic acid to make the skin plump, full, young and firm. As we age, the skin's internal nutrients (raw materials) are lost, and maternal cells gradually lose their vitality (original power). The "collagen manufacturing machine" has insufficient production capacity, collagen is lost, collagen breaks, and the skin loses its elastic support, resulting in sagging, depression, relaxation, and wrinkles. The principle is as follows: Figure 1 shown.

[0054] Small molecule kinetic energy can give collagen better micro-elasticity and stronger tensile strength. It contains more small molecule hydrolyzed collagen as small molecules, which can penetrate deep into the skin and provide nutrients needed by cells and skin. Small molecule collagen peptide can directly supply energy to the cell power source (mitochondria). With the blessing of this ingredient, the vitality of the mother cells of the "collagen manufacturing machine" is greatly improved, with full energy motive force, promoting the generation of "super collagen chain". Its principle is as follows Figure 2 shown.

[0055] Below, in conjunction with specific embodiments, the present invention is further described. It should be noted that, under the premise of no conflict, the embodiments described below or the technical features can be arbitrarily combined to form new embodiments. In the following embodiments, the components of the composition can be prepared by stirring evenly, and will not be described one by one in the following. Tianshan Saussurea Involucrata is white Tianshan Saussurea Involucrata.

[0056] Example 1

[0057] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:0.5, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0058] Example 2

[0059] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:0.8, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0060] Example 3

[0061] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:1, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0062] Example 4

[0063] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0064] Example 5

[0065] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:5, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0066] Example 6

[0067] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:8, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0068] Example 7

[0069] A composition consists of a small molecule kinetic energy element and a small molecule collagen peptide in a mass ratio of 1:10, wherein the small molecule kinetic energy element is sodium mannose phosphate, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0070] Example 8

[0071] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:0.5, wherein the small molecule kinetic energy is sodium mannose phosphate and passion flower extract in a mass ratio of 1:0.1, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0072] Example 9

[0073] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate and passion flower extract in a mass ratio of 1:0.2, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0074] Example 10

[0075] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate and passion flower extract in a mass ratio of 1:0.3, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0076] Embodiment 11

[0077] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate and gentiana macrophylla extract in a mass ratio of 1:0.05, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0078] Example 12

[0079] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate and gentiana macrophylla extract in a mass ratio of 1:0.1, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

[0080] Example 13

[0081] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate, passion flower extract and gentiana macrophylla extract in a mass ratio of 1:0.1:0.1, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0082] Embodiment 14

[0083] A composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:2, wherein the small molecule kinetic energy is sodium mannose phosphate, passion flower extract, gentiana macrophylla extract and snow lotus in a mass ratio of 1:0.1:0.1:0.01, and the small molecule collagen peptide is hydrolyzed collagen of giant salamander.

[0084] Performance Testing

[0085] 1. Transdermal penetration performance test

[0086] The International Cosmetic College of Shanghai University of Technology was commissioned to test the transdermal permeability of the composition of Example 3 in the anti-aging cell chip.

[0087] Testing instrument: SYSTEM918-12 dry heating automatic transdermal diffusion sampling system was used to measure the permeability of the composition of Example 3 in the M6P anti-aging cell chip sample, and the enzymatic method was used to measure the permeability of the composition of Example 3 at different times.

[0088] Sample preparation: Weigh 0.15 g of sample powder, dissolve it in 5 mL of PBS, and dilute it 10 times with PBS to obtain a 0.3% sample solution.

[0089] Experimental steps: (1) Install the 3M membrane and the quantitative ring between the donor pool and the receiving pool, use a pipette to accurately draw 300uL of 0.3% sample solution, put it in the center of the quantitative ring and spread it evenly, and set up 3 groups of parallel experiments; (2) Fill the receiving pool with PBS, expel excess bubbles, heat the diffusion pool to 37°C, and start the experiment. The capacity of the receiving pool is 12mL; (3) Take samples from the receiving pool at 1h, 2h, 4h, 8h, 12h, and 24h respectively and store them in a centrifuge tube at -80°C. The liquid collection program is set to take 6mL of liquid each time, and the remaining receiving liquid is discarded and then refilled with new PBS.

[0090] Test results: Figure 3-Figure 6 shown.

[0091] The test results show that, as shown in the figure, the permeability of the composition of Example 1 on the 3M membrane can reach about 80% within 1 hour, and the cumulative permeability remains basically unchanged after 12 hours. Therefore, the cumulative permeation amount of the composition of Example 3 in the M6P anti-aging cell chip sample in 24 hours is 1.36 mg, and the cumulative permeability is 97.57%.

[0092] 2. Elastase inhibition test

[0093] The samples were determined using the KMKZ TP-GZ04W-039 biochemical method - elastase inhibition rate.

[0094] Test sample: the composition of Example 3.

[0095] Reagents and materials: elastase, AAAPAN: N-succinyl-alanine-alanine-p-nitroanilide, positive control: EGCG, buffer: Tris-HCl pH 8.0.

[0096] Experimental operation: Elastase and the chromogenic substrate N-succinyl-alanine-alanine-p-nitroaniline were prepared into working solution using buffer solution, which was prepared temporarily; the positive control EGCG was prepared with buffer solution and diluted by half to form a series of concentrations to verify the test system; test substance treatment: the test substance was diluted with buffer solution to the required test concentration, and no dilution was required if there was no requirement; referring to the sample addition grouping list, a 96-well microplate was used for the test, and a sample group (T), a sample background group (T0), an enzyme reaction group (C) and a solvent background group (C0) were set up, where the positive control group and the sample group were treated the same, and 3 parallels were set up for each group. The results were calculated and the formula for calculating the inhibition rate of elastase activity was: inhibition rate (%) = [1-(T-T0) / (C-C0)]*100%, where: T-sample group absorbance, i.e., the absorbance of the solution after the sample affected the reaction of elastase and substrate AAAPAN; T0-sample substrate group absorbance; C-enzyme reaction group absorbance, i.e., the absorbance of the reaction of elastase and substrate AAAPAN when no sample was added; C0-solvent background group absorbance.

[0097] The test results are shown in Table 1.

[0098] Table 1

[0099]

[0100] Note: Compared with the blank group: *, p<0.05; **, p<0.01; ***, p<0.001; ****, p<0.0001;

[0101] The results showed that 1% of the composition of Example 3 had an inhibitory effect on the decomposition of AAAPAN catalyzed by elastase, with an inhibition rate of 14.11±3.4%, and the data had a significant difference (p=0.0081).

[0102] 3. Cell autophagy detection

[0103] Test system: human immortalized keratinocytes (HaCaT). Cell generation: at least 2 generations. Cell number for autophagy experiment: 3×10 5 Pieces / hole.

[0104] Test sample: the composition of Example 3.

[0105] Experimental steps: 1. The experiment was divided into a normal control group and a sample group. HaCaT cells in the logarithmic growth phase were taken and the concentration was 3×10 5 1. After incubation, remove the culture medium, add fresh culture medium containing samples to the sample group, and replace the culture medium with fresh culture medium to the normal control group, and continue to incubate at 37°C, 5% CO2 for 24 hours. 3. After incubation, use RIPA lysis buffer to lyse the cells and extract protein. 4. Detect the expression of LC3-Ⅱ protein and p62 protein by Western Blot method.

[0106] Test results: See Table 2-3 and Figure 7-9 The results showed that the sample had the effect of promoting cell autophagy.

[0107] Table 2

[0108] Detection concentration / % P62 / β-actin ratio p-value Test results 0.1 1.14 >0.05 Not significant 0.25 0.841 <0.01 Significant 0.75 0.727 <0.01 Significant

[0109] Table 3

[0110] Detection concentration / % LC3-Ⅱ / β-actin ratio p-value Test results 0.1 0.617 >0.05 Not significant 0.25 0.736 <0.01 Significant 0.75 0.819 <0.01 Significant

[0111] 4. Test to promote mitochondrial activity

[0112] Test system: human immortalized keratinocytes (HaCaT); cell generation: at least 2 generations; number of cells per group: 5×10 4 Pieces / hole.

[0113] The samples are: Example 4, Example 10, Example 12, Example 13, and Example 14.

[0114] Experimental steps: 1. Inoculate 5×10 4 / well of cells in a 24-well plate and cultured at 37℃ for 24±2h. 2. Drug administration, complete culture medium was added to the normal control wells and the model control wells, and complete culture medium containing samples of corresponding concentrations was added to the sample wells, and cultured at 37℃ for 4h. After the end, UVB modeling was performed. 3. After the modeling, fresh complete culture medium was replaced and cultured at 37℃ for 24±2h; after the end, JC-1 staining was performed and the cells were observed and photographed under a fluorescence microscope. 4. Image J was used to analyze the fluorescence intensity (S) of each group and calculate the decrease rate of mitochondrial membrane potential. The calculation formula is: Membrane potential decrease rate (%) = 100%*S (green fluorescence intensity) / S (red fluorescence intensity).

[0115] The test results are shown in Table 4 below.

[0116] Table 4

[0117] Detection Grouping Mitochondrial membrane potential decrease rate / % P-value Test results Normal control group 20 / / Model control group 48 / / Example 4 22 ＜0.001 Significant Example 10 25 ＜0.001 Significant Example 12 27 ＜0.001 Significant Example 13 32 ＜0.001 Significant Example 13 35 ＜0.001 Significant

[0118] The results showed that the compositions of Example 4, Example 10, Example 12, Example 13 and Example 14 could promote the increase of mitochondrial activity.

[0119] 5. In vitro firming and anti-wrinkle efficacy test

[0120] Instruments and materials: Human dermal fibroblasts (NHDFs) were purchased from ATCC; main reagents: PBS1871590, FM medium 21933, type I collagen ELISA kit M25032987, vitamin CSTBF1107V, 0.25% trypsin 1846496; main instruments: CO2 incubator 2015SW0058, biological safety cabinet 2015SW0026, inverted microscope 2015SW0056, microplate reader 2015SW0006, water bath 2015SW0023, mixer 2015SW0037.

[0121] The samples are: Example 4, Example 10, Example 12, Example 13, and Example 14.

[0122] Test method (ELISA method): Collect cells in the logarithmic growth phase and set the cell density at 6*10 4 Each well was inoculated into a 12-well plate and cultured in an incubator (37°C, 5% CO2) for 24 hours. Then, the sample drug diluted with distilled water was added, 100ug / ml vitamin C was used as the sample control, untreated cells were used as the negative control, and the reaction system reagents and solvents were used as the solvent control. Four replicate wells were set up in each group.

[0123] After adding the drug, the cells were cultured in an incubator (37°C, 5% CO2) for 48 h, the culture fluid was collected, and the type I collagen content in the culture fluid was detected using a COlⅠELISA kit. The relative content of type I collagen relative to the solvent control was calculated using the following formula: collagen content in the drug-treated well / collagen content in the negative control well = relative content of type I collagen.

[0124] The test results are shown in Table 5.

[0125] Table 5

[0126] project Relative content of type Ⅰ collagen Negative control 1.00±0.08 VC-100ug / ml 2.77±0.17 0.3% of Example 4 3.16±0.35 0.3% Example 10 3.25±0.26 0.3% Example 12 3.23±0.30 0.3% Example 13 3.68±0.14 0.3% Example 13 3.78±0.11

[0127] 6. In vitro antioxidant test

[0128] Main reagents: DPPH STBD4148V, vitamin C STBF1107V, anhydrous ethanol 20160401, microplate reader 2015SW0006, mixer 2015SW0037.

[0129] The samples are: Example 1, Example 4, Example 10, Example 12, Example 13, and Example 14.

[0130] Experimental content: Add distilled water to the sample to prepare a test solution with a concentration of 0.31%, prepare a reaction system according to the amount of each reagent added in Table 6, and mix well.

[0131] Table 6 DPPH free radical scavenging test reaction system

[0132] Unit / ul A1 A2 T1 T2 DPPH ethanol solution 500 0 500 0 Test liquid 0 0 500 500 Anhydrous ethanol 0 500 0 500 Distilled water / sample solvent 500 500 0 0

[0133] The reaction system was placed at room temperature and protected from light for 20 minutes. After the reaction was completed, 200ul of the reaction solution was added to a 96-well plate, and the absorbance OD value was read at 517nm. The scavenging rate of the sample for DPPH free radicals was calculated according to the following formula.

[0134] [(A1-A2)-(T1-T2)]*100% / (A1-A2) = scavenging rate of the sample for DPPH free radicals

[0135] Wherein: A1-blank absorbance value of DPPH system, A2-blank absorbance value of DPPH system, T1-sample group absorbance value of DPPH system, T2-sample group absorbance value of DPPH system.

[0136] The test results are shown in Table 7.

[0137] Table 7

[0138] project DPPH free radical scavenging rate / % Negative control 0.00±2.82 Example 1 15.79±1.59 Example 4 23.00±1.71 Example 10 40.75±0.59 Example 12 68.19±0.95 Example 13 73.44±2.61 Example 13 75.28±2.54

[0139] 7. NAD+ efficacy test

[0140] Cell line: human immortalized keratinocytes (HaCaT) Cell generation: at least 2 generations.

[0141] The samples are: Example 4, Example 10, Example 12, Example 13, and Example 14, which are diluted to 0.5% with distilled water.

[0142] Experimental steps: HaCaT cells stably expressing mCherry-FiNad and control probe mCherry-cpYFP were constructed and spread on ELISA plates and glass-bottom imaging culture dishes. Cells were treated with different samples for 24 hours and then ELISA quantification and imaging were performed.

[0143] The test results are shown in Table 8 below.

[0144] Table 8

[0145]

[0146] 8. Cell repair test

[0147] Main reagents: PBS1871590, FM medium21933, DMEM medium1859228, 0.25% trypsin1846496, BrdU kit17267000.

[0148] Main instruments: CO2 incubator 2015SW0058, biological safety cabinet 2015SW0026, inverted microscope 2015SW0056, microplate reader 2015SW0006, water bath 2015SW0023, mixer 2015SW0037.

[0149] The samples are: Example 1, Example 4, Example 10, Example 12, Example 13, and Example 14.

[0150] Test method: Collect cells in logarithmic growth phase and set the cell density to 8*10 3 Each well was inoculated into a 96-well plate, with 100ul of culture medium in each well. After culturing in an incubator (37°C, 5% CO2) for 24h, the sample drug diluted with distilled water was added, 10% FBS was used as a positive control, untreated cells were used as a negative control, and cells without BrdU treatment were used as a blank control. Six replicate wells were set up for each group.

[0151] After adding the drug, the cells were cultured in an incubator (37°C, 5% CO2) for 48 h, and then the BrdU experiment was performed. Three background controls were set for each concentration, and the absorbance OD value was read at 450 / 690 nm. The average OD value of the blank control should be less than 0.1. The relative cell proliferation ability was calculated according to the following formula.

[0152] The calculation formula is: (OD of drug-administered well-OD of drug-administered background)*100% / (OD of solvent-control well-OD of solvent-control background)=relative proliferation capacity.

[0153] The test results are shown in Table 9 below.

[0154] Table 9

[0155] project Relative proliferation capacity Negative control (untreated) 1.00±0.24 Positive control (10% FBS) 13.40±0.52 0.3% of Example 1 1.56±0.16 0.3% of Example 4 2.24±0.24 0.3% Example 10 2.35±0.32 0.3% Example 12 2.69±0.31 0.3% Example 13 4.53±0.27 0.3% Example 13 4.60±0.22

[0156] The above tests show that: using giant salamander hydrolyzed collagen as a small molecule collagen peptide and a component containing sodium mannose phosphate as a small molecule kinetic element can help promote the growth of collagen chains with better supporting structures and anti-oxidation, so as to repair cell photodamage.

[0157] The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantial changes and substitutions made by technicians in this field on the basis of the present invention shall fall within the scope of protection required by the present invention.

Claims

1. A composition having the effect of repairing light-damaged cells, characterized in that: The composition consists of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic energy comprises sodium mannose phosphate, and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

2. The composition having the effect of repairing cell light damage according to claim 1, characterized in that: The mass ratio of the small molecule kinetic energy element to the small molecule collagen peptide is 1:(2-4).

3. A composition having the effect of repairing light-damaged cells, characterized in that: The composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic energy comprises sodium mannose phosphate and passion flower extract in a mass ratio of 1: (0.1-0.3), and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

4. The composition having the effect of repairing cell light damage according to claim 3, characterized in that: The mass ratio of the sodium mannose phosphate to the passion flower extract is 1:0.

3.

5. A composition having the effect of repairing light-damaged cells, characterized in that: The composition is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10), wherein the small molecule kinetic energy comprises sodium mannose phosphate and qinjiao extract in a mass ratio of 1: (0.05-0.1), and the small molecule collagen peptide is giant salamander hydrolyzed collagen.

6. The composition having the effect of repairing photodamage to cells according to claim 5, characterized in that: The mass ratio of the sodium mannose phosphate to the gentiana macrophylla extract is 1:0.

1.

7. A composition having the effect of repairing light-damaged cells, characterized in that: The invention is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1: (0.5-10). The small molecule kinetic energy comprises sodium mannose phosphate, passion flower extract and qinjiao extract in a mass ratio of 1: (0.1-0.3): (0.05-0.1). The small molecule collagen peptide is hydrolyzed collagen of giant salamander.

8. The composition having the efficacy of repairing photodamage to cells according to claim 7, characterized in that: The mass ratio of the sodium mannose phosphate, the passion flower extract and the gentiana macrophylla extract is 1:0.1:0.

1.

9. A composition having the effect of repairing light-damaged cells, characterized in that: The invention is composed of small molecule kinetic energy and small molecule collagen peptide in a mass ratio of 1:(0.5-10). The small molecule kinetic energy comprises sodium mannose phosphate, passion flower extract, qinjiao extract and snow lotus in a mass ratio of 1:(0.1-0.3):(0.05-0.1):(0.01-0.1). The small molecule collagen peptide is hydrolyzed collagen of giant salamander.

10. Use of a composition having the effect of repairing photodamage to cells as claimed in any one of claims 1 to 9, characterized in that: The composition having the effect of repairing photodamaged cells is used in the preparation of cosmetics, and the cosmetics include essence milk, essence lotion, emulsion, facial cream and facial mask.

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