Special alpine medicinal plant cultivation microbial fertilizer for alpine regions

Abstract

The invention discloses a special alpine medicinal plant cultivation microbial fertilizer for alpine regions, and relates to the technical field of fertilizers. The microbial fertilizer for cultivation of the medicinal plants in high and cold areas is prepared from the following raw material components in parts by mass: 8 to 12 parts of humic acid, 2 to 4 parts of wormcast, 8 to 15 parts of sepiolite, 3 to 5 parts of modified biomass charcoal, 3 to 4 parts of a chenopodium glaucum root extract, 0.5 to 1.0 part of a cedarwood extract and 0.4 to 0.8 part of compound microorganisms, the compound microorganism is a mixture compounded by cold-resistant bacillus subtilis and brevibacterium brevicola according to the mass ratio of 3: 1. The microbial fertilizer disclosed by the invention can meet the nutritional requirements of the high-cold medicinal plants in a severe environment, the used raw materials are reasonably matched, and the microbial fertilizer effectively ensures the yield and quality of the high-cold medicinal plants and has important economic value in the field of planting of the high-cold medicinal plants.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizers, in particular to a microbial fertilizer for cultivating high-cold medicinal plants specially used in high-cold areas. Background Art

[0002] Alpine medicinal plants are medicinal plants that grow in high altitude and cold climates. These medicinal plants are drought-resistant and cold-resistant, and can survive and reproduce in harsh environments. The unique climatic conditions in alpine areas provide a unique growth environment for the growth of these medicinal plants, giving them unique advantages in medicinal value.

[0003] Alpine medicinal plants have unique growth advantages and market value under specific environments, but the environment in alpine mountainous areas is harsh. Extreme environments such as low temperature, hypoxia, and strong ultraviolet rays place extremely high demands on the cultivation of alpine medicinal plants. It is necessary to overcome environmental and technical challenges to ensure the quality and yield of medicinal plants. Summary of the invention

[0004] The purpose of the present invention is to provide a microbial fertilizer for cultivating high-cold medicinal plants specifically for high-cold areas, so as to solve the problems existing in the above-mentioned prior art and ensure the high quality and high yield of high-cold medicinal plants.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The present invention provides a microbial fertilizer for cultivating high-cold medicinal plants, comprising the following raw material components in parts by weight:

[0007] 8-12 parts of humic acid, 2-4 parts of earthworm castings, 8-15 parts of sepiolite, 3-5 parts of modified biochar, 3-4 parts of Chenopodium glaucum root extract, 0.5-1.0 parts of Zhongbin grass extract and 0.4-0.8 parts of composite microorganisms;

[0008] The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:1.

[0009] Furthermore, the preparation method of the modified biochar comprises the following steps:

[0010] The biomass material is carbonized once under anaerobic conditions and cooled to room temperature to obtain biomass charcoal;

[0011] The biomass charcoal is mixed with oxalic acid and citric acid in water for reaction, filtered, and dried. The obtained reaction product is subjected to secondary carbonization under anaerobic conditions and cooled to room temperature to obtain the modified biomass charcoal.

[0012] Preferably, the biomass material is wheat straw.

[0013] Furthermore, the temperature of the primary carbonization and the secondary carbonization is 550-600°C.

[0014] Furthermore, the temperature of the mixed reaction is room temperature and the time is 24-48 hours.

[0015] Furthermore, the mass ratio of the biochar to oxalic acid and citric acid is 30-50:3-4:4-5.

[0016] Furthermore, the preparation method of the extract of the Chinese wolfberry grass comprises the following steps:

[0017] The whole herb of Herba Lycopodiellae is added into an aqueous solution of citric acid and L-proline, and extracted at 85-88° C. to obtain the Herba Lycopodiellae extract.

[0018] Furthermore, the molar ratio of citric acid to L-proline is 1:1; and the extraction time is 1-1.5h.

[0019] Furthermore, the preparation method of the Chenopodium glaucum root extract comprises the following steps:

[0020] The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum;

[0021] The alcohol extract of the Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

[0022] The present invention also provides a method for preparing the above-mentioned high-cold medicinal plant cultivation microbial fertilizer, comprising the following steps:

[0023] The raw materials are mixed according to a mass ratio to obtain the alpine medicinal plant cultivation microbial fertilizer.

[0024] The present invention uses cold-resistant Bacillus subtilis and cold-resistant Brevibacillus as mixed bacteria, with Chenopodium glaucum root extract and Zhongbin grass extract, and compounded modified biomass charcoal, humic acid, earthworm castings and sepiolite, to ensure the metabolism of the mixed bacteria and the conversion and absorption of nutrients by high-cold medicinal plants.

[0025] The present invention discloses the following technical effects:

[0026] The microbial fertilizer of the present invention can meet the nutritional needs of high-altitude medicinal plants in harsh environments. The raw materials used are reasonably matched. The microbial fertilizer effectively guarantees the yield and quality of high-altitude medicinal plants and has important economic value in the field of high-altitude medicinal plant cultivation. DETAILED DESCRIPTION

[0027] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0028] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0029] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0030] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0031] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0032] The cold-resistant Bacillus subtilis used in the following examples of the present invention was purchased from the General Microbiological Center of China Microorganism Culture Collection Administration, with a deposit number of CGMCC NO.22039; the cold-resistant Brevibacillus used was purchased from the China Center for Type Culture Collection, with a deposit number of CCTCC No:M2013673.

[0033] Example 1

[0034] A microbial fertilizer for cultivating high-altitude medicinal plants, the raw materials have the following mass proportions:

[0035] 12 parts of humic acid, 3 parts of earthworm castings, 13 parts of sepiolite, 4 parts of modified biochar, 3 parts of Chenopodium glaucum root extract, 0.9 parts of Zhongbin grass extract and 0.6 parts of composite microorganisms;

[0036] The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:1.

[0037] (1) Preparation of modified biochar:

[0038] The wheat straw was crushed and carbonized once at 550°C under anaerobic conditions for 2 hours, and then cooled to room temperature to obtain biochar;

[0039] Biochar, oxalic acid and citric acid were added to 100 times the mass of water in a mass ratio of 35:4:4, mixed and reacted at room temperature for 48 hours, filtered and dried, and the obtained reaction product was secondary carbonized at 600°C under anaerobic conditions for 3 hours, and cooled to room temperature to obtain modified biochar.

[0040] (2) Preparation of Zhongbin grass extract:

[0041] Citric acid and L-proline were added to 10 times the mass of deionized water in a molar ratio of 1:1, heated at 85°C for 1 hour, and then the whole herb of Zhongbincao was added and extracted at 85°C for 1.5 hours.

[0042] (3) Preparation of Chenopodium album root extract:

[0043] The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum;

[0044] The alcohol extract of Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

[0045] (4) Preparation of microbial fertilizer for cultivation of high-altitude medicinal plants: Mix the above raw materials according to the mass ratio to obtain microbial fertilizer for cultivation of high-altitude medicinal plants.

[0046] Example 2

[0047] A microbial fertilizer for cultivating high-altitude medicinal plants, the raw materials have the following mass proportions:

[0048] 8 parts of humic acid, 4 parts of earthworm castings, 15 parts of sepiolite, 5 parts of modified biochar, 3 parts of Chenopodium glaucum root extract, 1.0 parts of Zhongbin grass extract and 0.8 parts of composite microorganisms;

[0049] The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:1.

[0050] (1) Preparation of modified biochar:

[0051] The wheat straw was crushed and carbonized once at 600°C in an anaerobic condition for 3 hours, and then cooled to room temperature to obtain biochar;

[0052] Biochar, oxalic acid and citric acid were added to 100 times the mass of water in a mass ratio of 50:4:5, mixed and reacted at room temperature for 24 hours, filtered and dried, and the obtained reaction product was secondary carbonized at 600°C under anaerobic conditions for 3 hours, and cooled to room temperature to obtain modified biochar.

[0053] (2) Preparation of Zhongbin grass extract:

[0054] Citric acid and L-proline were added to 10 times the mass of deionized water in a molar ratio of 1:1, heated at 88°C for 1 hour, and then the whole herb of Zhongbincao was added and extracted at 88°C for 1.5 hours.

[0055] (3) Preparation of Chenopodium album root extract:

[0056] The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum;

[0057] The alcohol extract of Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

[0058] (4) Preparation of microbial fertilizer for cultivation of high-altitude medicinal plants: Mix the above raw materials according to the mass ratio to obtain microbial fertilizer for cultivation of high-altitude medicinal plants.

[0059] Example 3

[0060] A microbial fertilizer for cultivating high-altitude medicinal plants, the raw materials have the following mass proportions:

[0061] 10 parts of humic acid, 4 parts of earthworm castings, 12 parts of sepiolite, 4 parts of modified biochar, 4 parts of Chenopodium glaucum root extract, 0.8 parts of Zhongbin grass extract and 0.6 parts of composite microorganisms;

[0062] The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:1.

[0063] (1) Preparation of modified biochar:

[0064] The wheat straw was crushed and carbonized once at 550°C under anaerobic conditions for 3 hours, and then cooled to room temperature to obtain biochar;

[0065] Biochar, oxalic acid and citric acid were added to 100 times the mass of water in a mass ratio of 45:4:5, mixed and reacted at room temperature for 24 hours, filtered and dried, and the obtained reaction product was secondary carbonized at 600°C under anaerobic conditions for 2 hours, and cooled to room temperature to obtain modified biochar.

[0066] (2) Preparation of Zhongbin grass extract:

[0067] Citric acid and L-proline were added to 10 times the mass of deionized water in a molar ratio of 1:1, heated at 88°C for 1 hour, and then the whole herb of Zhongbincao was added and extracted at 88°C for 1 hour.

[0068] (3) Preparation of Chenopodium album root extract:

[0069] The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum;

[0070] The alcohol extract of Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

[0071] (4) Preparation of microbial fertilizer for cultivation of high-altitude medicinal plants: Mix the above raw materials according to the mass ratio to obtain microbial fertilizer for cultivation of high-altitude medicinal plants.

[0072] Example 4

[0073] A microbial fertilizer for cultivating high-altitude medicinal plants, the raw materials have the following mass proportions:

[0074] 12 parts of humic acid, 3 parts of earthworm castings, 15 parts of sepiolite, 3 parts of modified biochar, 3 parts of Chenopodium glaucum root extract, 0.5 parts of Zhongbin grass extract and 0.4 parts of composite microorganisms;

[0075] The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:1.

[0076] (1) Preparation of modified biochar:

[0077] The wheat straw was crushed and carbonized once at 550°C under anaerobic conditions for 2 hours, and then cooled to room temperature to obtain biochar;

[0078] Biochar, oxalic acid and citric acid were added to 100 times the mass of water in a mass ratio of 30:3:4, mixed and reacted at room temperature for 24 hours, filtered and dried, and the obtained reaction product was secondary carbonized at 550°C under anaerobic conditions for 2 hours, and cooled to room temperature to obtain modified biochar.

[0079] (2) Preparation of Zhongbin grass extract:

[0080] Citric acid and L-proline were added to 10 times the mass of deionized water in a molar ratio of 1:1, heated at 85°C for 1 hour, and then the whole herb of Zhongbincao was added and extracted at 85°C for 1 hour.

[0081] (3) Preparation of Chenopodium album root extract:

[0082] The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum;

[0083] The alcohol extract of Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

[0084] (4) Preparation of microbial fertilizer for cultivation of high-altitude medicinal plants: Mix the above raw materials according to the mass ratio to obtain microbial fertilizer for cultivation of high-altitude medicinal plants.

[0085] Comparative Example 1

[0086] The only difference from Example 1 is that the cold-resistant Brevibacillus is replaced with cold-resistant Bacillus subtilis of equal mass.

[0087] Comparative Example 2

[0088] The only difference from Example 1 is that during the preparation of the modified biochar, oxalic acid is replaced with an equal mass of malic acid.

[0089] Comparative Example 3

[0090] The only difference from Example 1 is that the roots of Chenopodium album are extracted by water decoction, and the steps are as follows:

[0091] The roots of Quinoa gracile are decocted, and the decoction is collected and concentrated to dryness.

[0092] Comparative Example 4

[0093] The only difference from Example 1 is that ethyl acetate is used for extraction during the preparation of the Chenopodium glaucum root extract.

[0094] Effect verification example 1

[0095] 1. Experimental location: Qinghai Province.

[0096] 2. Experimental grouping: A certain Platycodon grandiflorum planting area in Qinghai was divided into 9 equal-sized plots (20 m 3 ), as experimental groups 1-4, control groups 1-4 and blank control group, respectively;

[0097] 3. Fertilization method: Test groups 1-4 were respectively applied with the microbial fertilizers prepared in Examples 1-4; control groups 1-4 were respectively applied with the microbial fertilizers prepared in Comparative Examples 1-4; and the blank control group was not applied with any fertilizer.

[0098] The experimental group and the control group applied microbial fertilizer as base fertilizer at a rate of 180 kg / mu before planting Platycodon grandiflorum.

[0099] Each group was planted at a sowing rate of 3kg / mu, and the field management measures were the same for each group.

[0100] After the Platycodon grandiflorum matured, the saponin content in each group of Platycodon grandiflorum was determined and the growth of Platycodon grandiflorum was recorded. The results are shown in Table 1.

[0101] Table 1

[0102] Yield (kg / mu) Plant height(cm) Root length (cm) Root diameter (cm) Total saponins from Platycodon grandiflorum(%) Example 1 337.8 65.6 25.4 24.3 6.94 Example 2 336.5 65.3 25.1 24.2 6.93 Example 3 337.1 65.4 25.3 24.2 6.90 Example 4 336.4 65.1 25.0 24.2 6.87 Comparative Example 1 248.5 52.3 19.2 18.9 5.85 Comparative Example 2 285.4 58.7 22.8 22.4 6.30 Comparative Example 3 272.1 56.5 20.2 20.9 6.11 Comparative Example 4 259.8 53.8 20.7 20.1 6.02 Blank control group 193 47.2 17.8 15.7 5.42

[0103] Effect verification example 2:

[0104] 1. Experimental location: Qinghai Province.

[0105] 2. Experimental grouping: The safflower planting area was divided into 9 equal-sized plots (15 m 3 ), as experimental groups 1-4, control groups 1-4 and blank control group, respectively;

[0106] 3. Fertilization method:

[0107] The test groups 1-4 were respectively administered with the microbial fertilizers prepared in Examples 1-4; the control groups 1-4 were respectively administered with the microbial fertilizers prepared in Comparative Examples 1-4; and the blank control group was not administered with any fertilizer.

[0108] The experimental group and the control group applied the microbial fertilizer evenly on the soil surface at an application rate of 150kg / mu before sowing safflower, and then tilled the soil.

[0109] Each group was planted at a sowing rate of 3kg / mu, and field management was carried out according to conventional management methods.

[0110] The test results are shown in Table 2.

[0111] Table 2

[0112]

[0113] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A microbial fertilizer for cultivating high-altitude medicinal plants, characterized in that: The invention comprises the following raw material components in parts by weight: 8-12 parts of humic acid, 2-4 parts of earthworm castings, 8-15 parts of sepiolite, 3-5 parts of modified biochar, 3-4 parts of Chenopodium glaucum root extract, 0.5-1.0 parts of Zhongbin grass extract and 0.4-0.8 parts of composite microorganisms; The composite microorganism is a mixture of cold-resistant Bacillus subtilis and cold-resistant Brevibacillus in a mass ratio of 3:

1.

2. The alpine medicinal plant cultivation microbial fertilizer according to claim 1, characterized in that: The preparation method of the modified biochar comprises the following steps: The biomass material is carbonized once under anaerobic conditions and cooled to room temperature to obtain biochar; The biomass charcoal is mixed with oxalic acid and citric acid in water for reaction, filtered, and dried. The obtained reaction product is subjected to secondary carbonization under anaerobic conditions and cooled to room temperature to obtain the modified biomass charcoal.

3. The alpine medicinal plant cultivation microbial fertilizer according to claim 2, characterized in that: The temperature of the primary carbonization and the secondary carbonization is 550-600°C.

4. The alpine medicinal plant cultivation microbial fertilizer according to claim 2, characterized in that: The temperature of the mixed reaction is room temperature and the time is 24-48 hours.

5. The alpine medicinal plant cultivation microbial fertilizer according to claim 2, characterized in that: The mass ratio of the biomass charcoal to oxalic acid and citric acid is 30-50:3-4:4-5.

6. The alpine medicinal plant cultivation microbial fertilizer according to claim 1, characterized in that: The preparation method of the Zhongbin grass extract comprises the following steps: The whole herb of Herba Lycopodiellae is added into an aqueous solution of citric acid and L-proline, and extracted at 85-88° C. to obtain the Herba Lycopodiellae extract.

7. The alpine medicinal plant cultivation microbial fertilizer according to claim 6, characterized in that: The molar ratio of the citric acid to L-proline is 1:1; and the extraction time is 1-1.5 hours.

8. The alpine medicinal plant cultivation microbial fertilizer according to claim 1, characterized in that: The preparation method of the Chenopodium glaucum root extract comprises the following steps: The dried roots of Chenopodium glaucum are added into anhydrous ethanol for extraction, and the anhydrous ethanol is removed by concentrating the extract to obtain an alcohol extract of the roots of Chenopodium glaucum; The alcohol extract of the Chenopodium glaucum root is dissolved in water, petroleum ether is added for extraction, and the organic phase is concentrated and dried to obtain the Chenopodium glaucum root extract.

9. The method for preparing the microbial fertilizer for cultivating high-cold medicinal plants according to any one of claims 1 to 8, characterized in that: The following steps are involved: The raw materials are mixed according to a mass ratio to obtain the alpine medicinal plant cultivation microbial fertilizer.

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