Cleaning agent for cleaning protein scales on inner wall of pipeline and application of cleaning agent

Abstract

The invention relates to a cleaning agent for cleaning protein scale on the inner wall of a pipeline, and relates to the technical field of cleaning agents, the cleaning agent comprises the following components by weight: 10-15 parts of sodium hydroxide, 20-30 parts of absolute ethyl alcohol, 0.5-2 parts of a surfactant, 0.1-1 part of alkaline protease, and 0.1-1 part of a chelating agent. By reasonably optimizing the proportion of the sodium hydroxide, the absolute ethyl alcohol, the lauryl sodium sulfate, the alkaline protease and the chelating agent, an efficient, safe and environment-friendly cleaning scheme is provided, the defects of the prior art in the aspect of protein sediment removal are overcome, and the cleaning agent is suitable for various industrial pipelines and equipment and also suitable for various industrial pipelines and equipment. Equipment loss and cleaning cost are greatly reduced, and the device has wide popularization value and is particularly suitable for industries with high cleanliness requirements, such as food processing and pharmacy.

Description

Technical Field

[0001] The invention relates to the technical field of cleaning agents, in particular to a cleaning agent for cleaning protein scale on the inner wall of a pipeline and application thereof. Background Art

[0002] The deposition of proteinaceous substances on the inner wall of pipes is a common problem in food processing, pharmaceutical industry and other related industrial fields. These protein deposits will gradually harden over time, forming stubborn scale, resulting in reduced flow capacity of the pipes. In severe cases, it may even cause blockage, increase equipment maintenance costs and cause production downtime. In addition, these deposits may also provide a breeding ground for bacteria and microorganisms, further endangering the health and safety of the production process.

[0003] In the prior art, the means of removing protein scale mainly include mechanical cleaning and chemical cleaning. Mechanical cleaning usually uses high-pressure water jets or mechanical brushes, but its effect is limited in complex piping systems, especially it is difficult to reach hidden parts inside the pipes. At the same time, mechanical cleaning may also cause wear of the pipe material and shorten its service life.

[0004] Chemical cleaning mainly relies on acidic or alkaline solutions and enzyme cleaning agents. Acidic cleaning agents are effective in removing inorganic mineral scale, but are less effective in removing protein deposits and are prone to corroding metal pipes; in contrast, alkaline cleaning agents, such as sodium hydroxide (NaOH), can effectively dissolve proteins, but high-concentration NaOH solutions are not only highly corrosive, posing safety hazards to operators and equipment, but also polluting the environment and increasing the complexity and cost of waste liquid treatment.

[0005] In recent years, enzyme cleaners have been widely recognized as environmentally friendly cleaning solutions due to their highly specific ability to hydrolyze proteins. However, enzyme cleaners are relatively expensive and sensitive to temperature and pH, which limits their popularity in industrial applications. When enzyme cleaners are used alone, they are often unable to completely remove stubborn protein deposits and still need to be used in conjunction with other cleaning methods.

[0006] In order to improve cleaning efficiency and reduce equipment corrosion and environmental pollution, researchers have tried to optimize the cleaning agent formula through composite additives or material improvements. For example, adding surfactants can enhance the wettability and permeability of sodium hydroxide, and chelating agents can help reduce the corrosion of metal pipes. However, the implementation of these solutions is often costly and complicated, which limits their widespread application. Summary of the invention

[0007] The purpose of the present invention is to provide a cleaning agent for cleaning protein scale on the inner wall of a pipeline, which overcomes the problems of low cleaning efficiency, serious equipment corrosion and environmental pollution in the prior art. The formula can not only maintain excellent solubility under conditions of 70°C to 80°C, but also has the advantages of low operating costs and reduced equipment wear, and is suitable for a variety of industrial applications.

[0008] The cleaning agent of the present invention is specially designed for removing protein deposits on the inner wall of a pipeline, and has higher cleaning efficiency and environmental friendliness. Compared with the prior art, the present invention can efficiently remove protein deposits in a pipeline at a medium temperature by optimizing the ratio of sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate and alkaline protease; SDS, as a surfactant, can destroy the hydrophobic structure of protein, enhance the permeability of NaOH, and make the protein more easily soluble; alkaline protease efficiently catalyzes the hydrolysis reaction of protein in an alkaline environment, ensuring that the protein is completely decomposed; the addition of a chelating agent (such as ethylenediaminetetraacetic acid or citric acid) further reduces the corrosion of metal pipelines during the cleaning process, thereby extending the service life of the equipment.

[0009] The cleaning formula of the present invention is not only highly efficient and safe to operate, but also simple to treat the waste liquid after cleaning, which complies with the "National Integrated Wastewater Discharge Standard" (GB 8978-1996), reduces the impact on the environment, and by reducing the concentration of NaOH used, the optimized formula ensures efficient cleaning while reducing potential hazards to personnel and equipment.

[0010] The present invention provides an efficient, economical and environmentally friendly solution by improving the existing cleaning technology. It is particularly suitable for cleaning complex pipeline systems in industrial fields such as food processing and pharmaceuticals. The cleaning agent can not only significantly improve the cleaning efficiency, but also reduce the damage to the pipeline material, and has broad application prospects.

[0011] To achieve the above-mentioned purpose, the present invention provides a cleaning agent for cleaning protein scale on the inner wall of a pipeline, characterized in that it comprises the following components, calculated by weight: 10-15 parts of sodium hydroxide, 20-30 parts of anhydrous ethanol, 0.5-2 parts of a surfactant, 0.1-1 parts of an alkaline protease, and 0.1-1 parts of a chelating agent.

[0012] Preferably, in the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the surfactant is sodium dodecyl sulfate.

[0013] Preferably, in the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the source of the alkaline protease is selected from filamentous fungi or Bacillus subtilis.

[0014] Preferably, in the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the chelating agent is selected from one or both of ethylenediaminetetraacetic acid and citric acid.

[0015] The present invention also provides an application of a cleaning agent for cleaning protein scale on the inner wall of a pipeline, wherein the cleaning agent is applied to cleaning the pipeline.

[0016] Preferably, the use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline, wherein the use of the cleaning agent for cleaning the pipeline specifically comprises the following steps:

[0017] Step S1. Weighing sodium hydroxide, anhydrous ethanol, a surfactant, an alkaline protease and a chelating agent according to the weight of each component, and mixing the above components to obtain a cleaning agent;

[0018] Step S2. Heat the cleaning agent and inject it into the pipeline, stir and clean it;

[0019] Step S3. Rinse the pipe cleaned in step S2 with clean water and dispose of the waste liquid.

[0020] Preferably, in the use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the heating temperature in step S2 is 50° C.-80° C. and the cleaning time is 10-20 min.

[0021] Preferably, in the use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the heating temperature in step S2 is 70° C.-80° C.

[0022] Preferably, in the use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the stirring speed in step S2 is 200-800 rpm.

[0023] Preferably, in the use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline, the stirring speed in step S2 is 600 rpm.

[0024] Sodium hydroxide provides a strong alkaline environment to destroy the secondary and tertiary structures of proteins; anhydrous ethanol is used as a solvent and auxiliary agent to improve the dispersibility and permeability of NaOH and enhance the protein dissolution effect; sodium dodecyl sulfate is used as a surfactant to destroy the hydrophobic structure of proteins and promote the interaction between detergents and protein molecules; alkaline protease catalyzes protein hydrolysis in an alkaline environment and decomposes proteins into small peptides and amino acids. Alkaline protease is selected from filamentous fungi or Bacillus subtilis. Alkaline protease derived from filamentous fungi has excellent ability to hydrolyze proteins in a wider pH range, especially under alkaline conditions; and alkaline protease derived from Bacillus subtilis exhibits excellent stability and activity under high temperature and high alkaline conditions. , which is particularly suitable for the rapid decomposition and removal of protein deposits in industrial pipelines; chelating agents form stable chelates with metal ions released during the cleaning process, which inhibit the reactivity of metal ions from the molecular structure, prevent them from adversely reacting with cleaning agents, and thus protect pipeline materials from corrosion; chelating agents tightly bind to metal ions through their multiple coordination groups to form stable cyclic or polycyclic structures, which significantly reduce the mobility and reactivity of metal ions. Taking ethylenediaminetetraacetic acid (EDTA) as an example, it can form highly stable chelates with a variety of metal ions (such as calcium, magnesium, iron, etc.), ensuring that metal ions will not react with other components in the cleaning agent during the cleaning process, thereby effectively inhibiting corrosion and extending the service life of the pipeline.

[0025] NaOH and SDS work synergistically under alkaline conditions. SDS destroys the hydrophobic structure of proteins, making it easier for protein molecules to come into contact with alkaline proteases and further hydrolyze them. Especially in the complex sediment environment in pipes, alkaline proteases derived from filamentous fungi and Bacillus subtilis can maintain high activity under alkaline conditions and at higher temperatures, significantly accelerating the decomposition of protein deposits and improving cleaning efficiency. This allows protein molecules to expose more enzyme action sites, and alkaline proteases further catalyze hydrolysis, accelerating the cleaning speed.

[0026] The optimal operating temperature range of the cleaning agent is 50°C to 80°C, especially at 70°C to 80°C, the dissolution efficiency is best, which can effectively shorten the cleaning time and is suitable for industrial production where strict requirements on cleaning time and efficiency are placed; the stirring speed of the cleaning solution is 200rpm to 800rpm, with the optimal being 600rpm, to ensure that the solution is evenly distributed in the pipe and fully in contact with the protein deposits.

[0027] The addition of chelating agents can effectively reduce corrosion in metal piping systems and extend the service life of equipment, making this formula suitable for a variety of piping materials such as stainless steel, plastic, and glass, and is particularly suitable for industrial environments that require frequent cleaning.

[0028] The cleaning agent can effectively dissolve protein deposits on the inner wall of the pipe in a short time and achieve efficient cleaning at a lower concentration, reducing damage to the pipe, reducing the amount of cleaning agent used and cleaning costs, and significantly reducing equipment maintenance costs.

[0029] The cleaning formula of the present invention has significant environmental protection characteristics. The waste liquid generated during the cleaning process has simple components and is easy to handle. It meets the requirements of the "National Comprehensive Wastewater Discharge Standard" (GB 8978-1996), reduces environmental pollution, and is suitable for industrial fields with strict environmental protection requirements.

[0030] The cleaning agent of the present invention is particularly suitable for industries with high requirements for cleanliness, such as food processing and pharmaceutical industries. The cleaning agent can quickly and efficiently dissolve protein deposits on the inner wall of the pipeline, ensure the smooth flow of the pipeline, and improve production efficiency and sanitary safety. The cleaning agent is not only suitable for cleaning protein deposits on the inner wall of the pipeline, but can also be used in industrial cleaning occasions such as equipment surfaces and container inner walls. Its wide applicability makes it have high application value in multiple industrial fields. The cleaning formula of the present invention shows high flexibility during the application process. According to specific application requirements, the concentrations of NaOH, SDS, anhydrous ethanol and enzymes can be adjusted to adapt to different types of deposits and cleaning tasks, so as to meet the cleaning needs of different industries.

[0031] Beneficial effects:

[0032] (1) The cleaning agent of the present invention can rapidly dissolve protein deposits at a moderate temperature of 50°C to 80°C through a reasonable combination of sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate and alkaline protease, thereby significantly improving the cleaning efficiency. Compared with traditional mechanical cleaning and single chemical cleaning, the present invention can achieve better cleaning effects in a short time.

[0033] (2) Compared with the cleaning solution of high concentration NaOH, the present invention effectively reduces the corrosion of metal pipes by reasonably reducing the concentration of NaOH and adding a chelating agent, thereby extending the service life of the equipment and being suitable for long-term industrial applications.

[0034] (3) The cleaning agent of the present invention has simple waste liquid composition after use and is easy to handle. It complies with the "National Comprehensive Wastewater Discharge Standard" (GB 8978-1996), significantly reducing the burden on the environment. Compared with traditional high-concentration acid-base cleaning agents, the present invention has less impact on the environment and has good environmental performance. The cleaning agent of the present invention can adjust the concentration ratio of NaOH, SDS and anhydrous ethanol according to cleaning requirements, adapt to different protein deposits and pipeline materials, and is widely applicable to various pipeline systems such as stainless steel, plastic, glass, etc., and has wide adaptability.

[0035] (4) By controlling the NaOH concentration and introducing a chelating agent, the safety risks of equipment and operators during the cleaning process are greatly reduced. Compared with the traditional high-concentration strong alkali or strong acid cleaning method, the cleaning process of the present invention is more gentle and safe, and is suitable for industries with high requirements for cleaning operation safety.

[0036] (5) The present invention reduces the amount of cleaning agent and equipment maintenance costs by optimizing the formula of the cleaning agent, thereby achieving an economical and efficient cleaning effect and reducing the cleaning cost.

[0037] (6) The present invention provides an efficient, safe and environmentally friendly cleaning solution by rationally optimizing the ratio of sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and chelating agent, thereby overcoming the shortcomings of the prior art in removing protein deposits. The cleaning agent is not only suitable for a variety of industrial pipelines and equipment, but also greatly reduces equipment loss and cleaning costs. It has a wide range of promotion value and is particularly suitable for industries with high cleanliness requirements such as food processing and pharmaceuticals. DETAILED DESCRIPTION

[0038] The present invention is described below by means of the best embodiments. It should be known to those skilled in the art that the embodiments are only used to illustrate the present invention rather than to limit the scope of the present invention.

[0039] Example 1

[0040] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 20 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 0.5 parts of ethylenediaminetetraacetic acid, and 5 parts, 10 parts and 15 parts of sodium hydroxide are added respectively, wherein the source of the alkaline protease is filamentous fungi.

[0041] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0042] Step S1. Weighing sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid according to the weight of each component, and mixing 5 parts, 10 parts and 15 parts of sodium hydroxide with anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid, respectively, to obtain a first cleaning agent, a second cleaning agent and a third cleaning agent;

[0043] Step S2. Place the protein deposit in a constant temperature environment of 45°C, add the first cleaning agent, the second cleaning agent and the third cleaning agent respectively, and continue stirring at a stirring speed of 400 rpm. Take samples every 10 minutes, measure the dissolution rate of the protein, and record the cleaning effect.

[0044] Protein deposits: simulate protein scale inside food processing equipment to ensure that the source of deposits is consistent with actual applications. Anhydrous ethanol is used to enhance the solubility and penetration of NaOH, sodium dodecyl sulfate (SDS) is used as a surfactant to promote protein dissolution, alkaline protease is used to catalyze protein hydrolysis, and chelating agents are used to inhibit the release of metal ions during the cleaning process to reduce corrosion to pipeline materials.

[0045] Experimental results: The dissolution rate of the first cleaning agent within 20 minutes was 70%, showing a slower dissolution rate; the dissolution rate of the second cleaning agent within 20 minutes reached 85%, showing a better dissolution effect; the third cleaning agent had the best dissolution effect, with a dissolution rate of 90% within 10 minutes and almost complete dissolution after 20 minutes. The first cleaning agent was used as a control to verify its inadequacy in protein dissolution at a lower concentration, further proving that the optimal NaOH portion of the present invention is 10-15 portions.

[0046] The experimental results show that 10-15 parts of NaOH addition is the optimal range for dissolving protein deposits; compared with 5 parts of NaOH solution, the higher concentration of NaOH significantly improves the cleaning efficiency, especially achieving a dissolution rate of more than 90% within 20 minutes, which verifies the high-efficiency cleaning performance of the present invention for protein deposits under moderate temperature conditions.

[0047] Example 2

[0048] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0049] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0050] Step S1. Weighing sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid according to the weight of each component, and mixing sodium hydroxide with anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid to obtain a fourth cleaning agent;

[0051] Step S2. Place the protein deposit in a constant temperature environment of 40°C, 50°C, 60°C, 70°C, and 80°C, add the fourth cleaning agent, and continue stirring at a stirring speed of 400 rpm. Take samples every 5 minutes, measure the dissolution rate of the protein, and record the cleaning effect.

[0052] Experimental results: In the temperature range of 70℃ to 80℃, the protein dissolution rate exceeded 95% within 10 minutes, showing an extremely high cleaning efficiency, which is significantly better than the dissolution effect under 40℃ and 50℃ conditions; at 50℃, the dissolution rate reached 85% after 20 minutes. Although the dissolution speed was relatively slow, it still had a good cleaning effect; at a lower temperature (40℃), the dissolution rate dropped significantly, and the dissolution rate within 30 minutes was only 60%, indicating that the cleaning efficiency was limited by temperature.

[0053] The experimental results show that the cleaning agent of the present invention exhibits the best protein dissolution efficiency in the range of 70°C to 80°C, and can quickly remove protein deposits in a short time; at the same time, although the cleaning speed is slower at 50°C, it still maintains a high dissolution capacity, verifying that the formula still has good cleaning performance under moderate temperature conditions; this proves the advantage of the cleaning agent in temperature adaptability and is suitable for pipeline cleaning applications in a variety of industrial environments.

[0054] Example 3

[0055] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0056] A blank control sample was set up, to which EDTA was not added, and the weight proportions of other components were the same.

[0057] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0058] Step S1. Sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid are weighed according to the weight of each component, and mixed to obtain a fifth cleaning agent. The stainless steel pipeline sample is immersed in the fifth cleaning agent and the blank control sample respectively, and the temperature is maintained at 70° C. and the immersion is continued for 20 minutes. The pipeline material is stainless steel 304, which is a common pipeline material in industrial applications, ensuring that the experimental results have practical reference value;

[0059] Step S2. After cleaning, the mass change of each pipeline sample before and after cleaning is measured, and the corrosion rate is evaluated to verify the inhibitory effect of the chelating agent on corrosion.

[0060] Experimental results: The corrosion rate of the cleaning solution without adding chelating agent on the stainless steel sample was 0.15%, showing a certain degree of corrosion; the fifth cleaning agent with 0.5% EDTA chelating agent added significantly reduced the corrosion rate to 0.05%, indicating that the chelating agent has a significant effect in inhibiting metal corrosion.

[0061] The experimental results show that the addition of chelating agent EDTA significantly reduces the corrosion of stainless steel pipes during the cleaning process, and the corrosion rate is reduced by about 66%. This not only prolongs the service life of the pipeline equipment, but also improves the safety of the cleaning process, verifying the effectiveness of the cleaning formula of the present invention in industrial applications.

[0062] Example 4

[0063] The protein deposits were cleaned with the fifth cleaning solution in Example 3, and the waste liquid generated after cleaning was collected to simulate the cleaning waste liquid in actual production.

[0064] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0065] Step S1. placing the protein sediment in a constant temperature environment of 70° C., adding the fifth cleaning agent, and continuously stirring at a stirring speed of 400 rpm for 10 min. After the cleaning is completed, the generated waste liquid is collected;

[0066] Step S2. Conduct a preliminary analysis on the waste liquid to determine its chemical oxygen demand, biochemical oxygen demand and pH value to evaluate its potential impact on the environment; neutralize the collected waste liquid, adjust the pH value to near neutral, measure the COD and BOD again, and evaluate the environmental compliance of the treated waste liquid.

[0067] Experimental results: Before treatment, the COD and BOD values ​​of the cleaning waste liquid were high and the pH value was alkaline; but after neutralization treatment, the COD value was reduced to below 50 mg / L, the BOD value was reduced to below 30 mg / L, and the pH value was close to neutral; the test results of the waste liquid after neutralization treatment met the requirements of the "National Integrated Wastewater Discharge Standard" (GB 8978-1996), indicating that the treated waste liquid has a lower environmental impact and meets the discharge standards.

[0068] The experimental results show that the waste liquid generated by the cleaning agent of the present invention after use can meet the national environmental protection emission standards through simple neutralization treatment; this not only simplifies the waste liquid treatment process, but also significantly reduces the cost of waste liquid treatment and the risk of environmental pollution, proving the environmental advantages of the cleaning formula in practical applications.

[0069] Example 5

[0070] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0071] Traditional cleaning formula: 20% NaOH combined with mechanical cleaning is used to simulate the currently commonly used industrial cleaning method. The specific operation of the traditional cleaning method is as follows: First, heat the 20% NaOH solution to 60°C, and then inject it into the pipeline system. Clean it with a mechanical brush or high-pressure water jet to help the NaOH solution contact and remove protein deposits. Keep the mechanical brush in contact with the inner wall of the pipeline during the entire cleaning process to increase the physical effect of cleaning.

[0072] Experimental steps:

[0073] Step S1. An equal amount of protein sediment samples are divided into two groups and respectively placed in two sets of equipment for cleaning: one group uses the fifth cleaning agent of Example 3 of the present invention, and the other group uses a traditional cleaning method (chemical cleaning combined with mechanical cleaning). In the control experiment, the two groups of cleanings are kept at the same temperature (60° C.) and stirring speed to ensure the consistency of the experimental conditions and the accuracy of the comparison of the cleaning effects;

[0074] Step S2. Record the protein dissolution of the two groups in different cleaning time periods, determine the dissolution rate, and compare the required cleaning time.

[0075] Experimental results: The sample using the fifth cleaning agent of Example 4 of the present invention completely dissolved the protein deposits within 30 minutes, with a dissolution rate of more than 99%, without the need for mechanical assistance; the sample using the traditional cleaning method, although combined with mechanical cleaning, only had a dissolution rate of 85% after 30 minutes, and the operation complexity of the equipment was relatively high.

[0076] The experimental results show that compared with traditional cleaning technologies, the cleaning agent of the present invention has significant advantages in cleaning efficiency; without the need for mechanical assistance, the cleaning agent of the present invention can not only achieve a higher dissolution rate in a shorter time, but also simplify the operation process and reduce the use and maintenance costs of the equipment. This result verifies the efficiency, safety and convenience of the cleaning formula of the present invention in industrial applications.

[0077] Example 6

[0078] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0079] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0080] Step S1. Place the protein sediment in a 70°C constant temperature reactor, add the fifth cleaning agent, and clean it at stirring speeds of 200 rpm, 400 rpm, 600 rpm and 800 rpm, respectively. Take samples every 10 minutes, measure the dissolution rate of the protein, and record the dissolution effect at different stirring speeds.

[0081] Experimental results: At a stirring speed of 200 rpm, the dissolution rate of protein deposits was slow, with the dissolution rate being only 80% after 30 minutes; at stirring speeds of 400 rpm and 600 rpm, the dissolution rate increased significantly, with the dissolution rates being 90% and 95% after 20 minutes, respectively; although the dissolution effect was improved at a stirring speed of 800 rpm, mechanical damage or excessive foaming may occur due to the excessively fast stirring speed, thus affecting the stability and effect of cleaning.

[0082] The experimental results show that 600 rpm is the optimal stirring speed, which can achieve a protein solubility rate of more than 95% within 20 minutes and avoid the side effects caused by excessively high stirring speeds, such as mechanical damage or foam affecting the cleaning efficiency; the experiment verifies the rationality of the stirring speed range of 200-800 rpm, especially 600 rpm as the optimal cleaning speed, which has superior performance in practical applications.

[0083] Example 7

[0084] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0085] A blank control sample was set up, to which no EDTA was added, and the cleaning agent and the blank control sample were used to clean pipe samples made of stainless steel, carbon steel and aluminum, respectively.

[0086] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0087] Step S1. Sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid are weighed according to the weight of each component, and mixed to obtain a fifth cleaning agent. Pipe samples made of stainless steel, carbon steel and aluminum are immersed in the fifth cleaning agent and a blank control sample, respectively, and the temperature is maintained at 70° C., and the immersion is continued for 20 minutes;

[0088] Step S2. After cleaning, the mass change of each pipeline sample before and after cleaning is measured, and the corrosion rate is evaluated to verify the inhibitory effect of the chelating agent on corrosion.

[0089] Experimental results: The corrosion rate of the stainless steel pipe group without chelating agent was 0.12%, and after adding ethylenediaminetetraacetic acid chelating agent, the corrosion rate dropped to 0.03%; the corrosion rate of the carbon steel pipe group without chelating agent was 0.25%, and after adding chelating agent, the corrosion rate was significantly reduced to 0.08%; the corrosion rate of the aluminum pipe group without chelating agent was 0.18%, and after adding chelating agent, the corrosion rate was reduced to 0.05%.

[0090] This experiment shows that EDTA can significantly reduce the corrosion of metal pipes during the cleaning process, especially the anti-corrosion effect on carbon steel and aluminum pipes. Compared with stainless steel, carbon steel and aluminum pipes are more sensitive to corrosion. Therefore, the addition of EDTA shows a stronger anti-corrosion effect on these materials, verifying its wide applicability in the cleaning of different metal materials.

[0091] Example 8

[0092] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0093] This experimental example evaluates the cleaning effect of the fifth cleaning agent of Example 3 when treating complex organic deposits (such as a mixture of proteins and lipids) and explores the applicability of the formula to different types of deposits.

[0094] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0095] Step S1. preparing a composite sediment sample, wherein the sample is composed of a mixture of 40% protein and 60% lipid components, simulating the complex sediment that may be formed in an industrial pipeline.

[0096] Step S2. Place the composite sediment in a constant temperature reactor at 70° C. and use the fifth cleaning agent for cleaning. Maintain the stirring speed at 600 rpm. Take samples every 10 minutes to measure the dissolution rates of protein and lipid components in the sediment and evaluate the cleaning effect.

[0097] Experimental results: After 20 minutes, the dissolution rate of the protein part in the composite sediment reached more than 90%, while the dissolution rate of the lipid component was only 50%; after 30 minutes, the protein part was completely dissolved, and the dissolution rate of the lipid component increased to 70%, but it was still relatively low.

[0098] The experimental results show that the cleaning agent of the present invention has an excellent cleaning effect on protein deposits and can achieve efficient dissolution in a relatively short time; however, the cleaning effect on lipid deposits is relatively limited. This shows that the cleaning agent is mainly targeted at cleaning protein deposits. For deposits containing complex organic matter, if the solubility of lipid components needs to be improved, the concentration of surfactants (such as SDS) can be appropriately increased, or other cleaning aids can be combined to enhance the cleaning effect on lipid components.

[0099] Example 9

[0100] This experimental example selected the stainless steel piping system of a food processing plant. There were obvious protein deposits on the inner wall of the pipe. These scales mainly came from the residual protein in the food processing process, which had formed a solid scale layer, affecting production efficiency and increasing the difficulty of cleaning.

[0101] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 25 parts of anhydrous ethanol, 1 part of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungi.

[0102] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0103] Step S1. Weighing sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid according to the weight of each component, and mixing the above components to obtain a sixth cleaning agent;

[0104] Step S2. Heat the sixth cleaning agent to 70°C and inject it into the pipeline through a pump to ensure that the cleaning solution is in full contact with the protein deposits on the inner wall of the pipeline. Use a circulating pump system to maintain a stirring speed of 500 rpm to circulate the cleaning solution. Continue cleaning for 20 minutes. Check the cleaning effect in the pipeline every 5 minutes and record the dissolution of the protein deposits. After cleaning, rinse the pipeline thoroughly with clean water to check whether the protein deposits are completely removed.

[0105] Experimental results: After 10 minutes, the protein scale in the pipeline began to dissolve significantly, and most of the sediment fell off; after 20 minutes, the protein deposits were completely dissolved, and the inner wall of the pipeline became clean and smooth again; after cleaning, there was no obvious corrosion or damage to the pipeline, and after flushing with clean water, the residual protein content was lower than the detection standard, meeting the production requirements.

[0106] The experimental results show that the cleaning agent of the present invention exhibits excellent cleaning performance in an actual food processing pipeline system and can efficiently remove stubborn protein scaling in a short time; no obvious damage is caused to the pipeline material during the cleaning process, indicating that the cleaning agent is suitable for frequent cleaning operations and has high safety and economy, and is suitable for wide promotion and application in the field of food processing.

[0107] Example 10

[0108] This experimental example selected a production pipeline of a pharmaceutical factory. The pipeline is made of stainless steel. The inner wall of the pipeline has been in contact with protein drug ingredients for a long time, forming stubborn protein deposits. These deposits not only affect the patency of the drug production line, but may also threaten the hygiene and quality requirements of the production process.

[0109] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 30 parts of anhydrous ethanol, 1.5 parts of sodium dodecyl sulfate, 0.5 parts of alkaline protease, 12 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. The source of the alkaline protease is filamentous fungus.

[0110] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0111] Step S1. Weighing sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate, alkaline protease and ethylenediaminetetraacetic acid according to the weight of each component, and mixing the above components to obtain a seventh cleaning agent;

[0112] Step S2. Heat the seventh cleaning agent to 60°C and inject it into the pharmaceutical pipeline through a pump to ensure that the cleaning solution is in full contact with the protein deposits on the inner wall of the pipeline. Use a circulating pump system to maintain a stirring speed of 600 rpm to circulate the cleaning solution. Continue cleaning for 20 minutes. Check the cleaning effect in the pipeline every 10 minutes and record the dissolution of protein deposits. After cleaning, rinse the pipeline with pure water and detect the residual protein content in the pipeline to ensure that it meets the hygiene requirements.

[0113] Experimental results: After 10 minutes, the protein deposits in the pipeline were partially dissolved, and the dissolution rate reached 60%; after 20 minutes, the protein deposits were completely dissolved, and there was no residue on the inner wall of the pipeline, and the cleaning effect reached the expected standard; during the cleaning process, no corrosion or damage to any pipeline material was observed, indicating that the cleaning formula of the present invention has good compatibility with pipeline materials.

[0114] Embodiment 11

[0115] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 20 parts of anhydrous ethanol, 1 part of sodium lauryl sulfate, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. In this embodiment, alkaline protease is not added.

[0116] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0117] Step S1: weighing sodium hydroxide, anhydrous ethanol, sodium dodecyl sulfate and ethylenediaminetetraacetic acid according to the weight of each component, and mixing them to obtain an eighth cleaning agent;

[0118] Step S2: Place the protein deposit in a constant temperature environment of 45° C., add the eighth cleaning agent, and continue stirring at 400 rpm for 20 minutes. Take samples every 10 minutes to measure the dissolution rate of the protein and record the cleaning effect.

[0119] Experimental results: After 20 minutes of cleaning, the dissolution rate of protein deposits was 30%. In comparison, when Example 1 (containing both sodium dodecyl sulfate and alkaline protease) was used under the same temperature and stirring conditions, the dissolution rate of protein deposits exceeded 90%. Therefore, this example shows that in the absence of alkaline protease, the cleaning effect is significantly reduced, further verifying the significant cleaning advantage of the synergistic use of alkaline protease and sodium dodecyl sulfate.

[0120] Example 12

[0121] A cleaning agent for cleaning protein scale on the inner wall of a pipeline comprises the following components, calculated by weight: 20 parts of anhydrous ethanol, 0.5 parts of alkaline protease, 15 parts of sodium hydroxide, and 0.5 parts of ethylenediaminetetraacetic acid. In this embodiment, sodium dodecyl sulfate is not added, and the source of the alkaline protease is filamentous fungi.

[0122] The application of cleaning agent to clean pipelines specifically includes the following steps:

[0123] Step S1: weighing sodium hydroxide, anhydrous ethanol, alkaline protease and ethylenediaminetetraacetic acid according to the weight of each component, and mixing them to obtain a ninth cleaning agent;

[0124] Step S2: Place the protein deposit in a constant temperature environment of 45° C., add the ninth cleaning agent, and continue stirring at 400 rpm for 20 minutes. Take samples every 10 minutes to measure the dissolution rate of the protein and record the cleaning effect.

[0125] Experimental results: After 20 minutes of cleaning, the dissolution rate of protein deposits was 25%. Under the same conditions, the dissolution rate of Example 1 exceeded 90%. The experimental results show that the cleaning effect of the detergent is significantly reduced when sodium dodecyl sulfate is lacking. The comparison with Example 1 verifies the enhanced effect of the combined action of sodium dodecyl sulfate and alkaline protease on the cleaning of protein deposits, indicating that the synergistic use of the two can achieve a better cleaning effect.

[0126] The cleaning agent of the present invention has shown a significant cleaning effect in the cleaning of pharmaceutical industry pipelines, especially at medium temperatures, it can efficiently remove stubborn protein deposits, and has no adverse effects on pipeline materials during the cleaning process. It meets the strict requirements of the pharmaceutical industry for hygiene, safety and cleanliness, and is suitable for promotion and application in the pharmaceutical field.

[0127] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A cleaning agent for cleaning protein scale on the inner wall of a pipeline, characterized in that: Calculated by weight, the composition includes: 10-15 parts of sodium hydroxide, 20-30 parts of anhydrous ethanol, 0.5-2 parts of a surfactant, 0.1-1 parts of alkaline protease and 0.1-1 parts of a chelating agent.

2. The cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 1, characterized in that: The surfactant is selected from one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, alkyl glucoside, and polyoxyethylene ether surfactants.

3. The cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 1, characterized in that: The source of the alkaline protease is selected from filamentous fungi or Bacillus subtilis.

4. The cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 1, characterized in that: The chelating agent is selected from one or both of ethylenediaminetetraacetic acid and citric acid.

5. Use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to any one of claims 1 to 4, characterized in that: Cleaning agents are used to clean pipes.

6. The use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 5, characterized in that: The application of cleaning agent to clean pipelines specifically includes the following steps: Step S1. Weighing sodium hydroxide, anhydrous ethanol, a surfactant, an alkaline protease and a chelating agent according to the weight of each component, and mixing the above components to obtain a cleaning agent; Step S2. Heat the cleaning agent and inject it into the pipeline, stir and clean it; Step S3. Rinse the pipe cleaned in step S2 with clean water and dispose of the waste liquid.

7. The use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 6, characterized in that: In step S2, the heating temperature is 50°C-80°C, and the cleaning time is 10-20 minutes.

8. The use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 7, characterized in that: The heating temperature in step S2 is 70°C-80°C.

9. The use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 6, characterized in that: The stirring speed in step S2 is 200-800 rpm.

10. The use of the cleaning agent for cleaning protein scale on the inner wall of a pipeline according to claim 9, characterized in that: The stirring speed in step S2 is 600 rpm.