Device and method for evaluating developmental toxicity of high-concentration cyanobacteria toxin based on fish embryos

Abstract

The invention relates to a fish embryo-based high-concentration cyanobacteria toxin development toxicity evaluation device and method, and relates to the technical field of pollutant toxicity analysis, the device structure comprises a base and an electric guide rail mechanism 1; the carrier is arranged on the first electric guide rail mechanism in a sliding mode, a movable oscillation base is arranged on the carrier, a plurality of perforated plates used for placing fish embryos are arranged on the movable oscillation base, and a lighting structure is arranged at the position, located below the movable oscillation base, of the carrier; the liquid injection unit is used for injecting cyanobacteria toxin working solutions with different high concentrations into the porous plates, and an observation unit is arranged at the top of the incubation chamber; and the adjusting shading unit is arranged between the lighting structure and the perforated plate. The evaluation device provided by the invention is convenient to use, reduces the labor intensity of workers, improves the working efficiency, and facilitates large-scale and high-efficiency evaluation and analysis of the fish embryonic development toxicity of the high-concentration cyanobacteria toxin.

Description

Technical Field

[0001] The invention belongs to the technical field of pollutant toxicity analysis, and specifically relates to a device and method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos. Background Art

[0002] As a new pollutant, cyanobacterial toxins (CYNs) are widely present in aquatic ecosystems and are difficult to degrade. At the same time, cyanobacterial toxins pose a potential threat to the ecological environment and human health through drinking water, aquatic products, and water recreational activities. Therefore, exploring the toxic effects and potential mechanisms of cyanobacterial toxins is of research significance for understanding the prevention and control of cyanobacterial toxins.

[0003] To explore the toxic effects of cyanobacterial toxins and their potential mechanisms, the effects of CYN on the early developmental toxicity, behavioral toxicity, and neurotoxicity of zebrafish can be evaluated by exposing fish embryos 4 hours after fertilization to different high concentrations of CYN and incubating them for a certain period of time, so as to further enrich the data on the toxicity of CYN, a cyanobacterial toxin, to aquatic organisms. Among them, the evaluation of early developmental toxicity is mostly carried out by recording the mortality rate, hatching rate, and deformity incidence of fish embryos. At the same time, during the recording process, it is necessary to promptly remove dead or deformed embryos to maintain the accuracy of the experiment.

[0004] At present, the evaluation process of early developmental toxicity is usually performed manually through a series of tedious steps, including injecting different high-concentration CYN solutions into the sample, transferring the sample to the incubator for incubation, and regular recording during the incubation process. The operation and observation are relatively troublesome, which increases the labor intensity and reduces the work efficiency, which is not conducive to the efficient developmental toxicity evaluation of high-concentration cyanobacterial toxins. Therefore, we propose a device and method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos. Summary of the invention

[0005] The purpose of the present invention is to provide a device and method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos in order to solve the above-mentioned problems.

[0006] The present invention achieves the above-mentioned purpose through the following technical solutions:

[0007] As a first aspect of the present invention, a device for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos is provided, comprising a machine base, and also comprising an electric guide rail mechanism extending along the length direction of the machine base into the incubation chamber;

[0008] A carrier slidably arranged on the electric guide rail mechanism, the carrier is provided with a movable oscillating seat and the movable oscillating seat is provided with a plurality of porous plates for placing fish embryos, and the carrier is provided with a lighting structure at a position below the movable oscillating seat for providing the required lighting conditions for the fish embryos;

[0009] An injection unit and an incubation chamber are arranged extending along the length direction of the machine base, wherein the injection unit is used to inject cyanobacteria toxin working solutions of different high concentrations into a plurality of porous plates, and an observation unit is arranged on the top of the incubation chamber;

[0010] And a shading adjustment unit is arranged between the lighting structure and the porous plate, which is used to adjust the contrast between the bottom of the porous plate and the environment, so as to facilitate the observation of the development of fish embryos through the observation unit.

[0011] As a further optimization scheme of the present invention, the loading rack includes a gantry and a panel arranged on the gantry, elastic telescopic parts arranged at both ends of the movable oscillation seat and movably connected to the panel, and a driving oscillation unit arranged on the gantry for driving the movable oscillation seat to realize oscillation.

[0012] As a further optimization scheme of the present invention, the driving oscillation unit includes a transmission rod and an eccentric connecting piece axially connected to the gantry, and the transmission rod rotates synchronously with the eccentric connecting piece through the transmission piece. When the eccentric connecting piece rotates, the outer end portion of the eccentric connecting piece intermittently and periodically pushes the movable oscillation seat to realize the oscillation swing of the movable oscillation seat.

[0013] As a further optimization scheme of the present invention, a hollow groove is provided on the movable oscillating seat at the position of the holes corresponding to the porous plate, and the structure of the adjustable shading unit includes an active roller, a winding roller and a shading cloth arranged between the active roller and the winding roller, and the shading cloth has a hollow portion.

[0014] As a further optimization scheme of the present invention, a multi-directional adjustment structure installed with an observation unit is provided on the top of the incubation chamber, and the structure of the multi-directional adjustment structure includes an elastic membrane arranged at the top of the incubation chamber, a mounting seat arranged on the elastic membrane to fix the observation unit, an annular slide rail arranged in the incubation chamber, a sliding block movably arranged in the annular slide rail, a telescopic rod with one end hinged to the outer side of the sliding block, and a hinged seat hinged to the other end of the telescopic rod, and the hinged seat is hinged to one end of the observation unit extending into the incubation chamber.

[0015] As a further optimization scheme of the present invention, the structure of the observation unit includes a cylinder connected to the multi-directional adjustment structure, an observation eye mask arranged at one end of the cylinder located outside the incubation chamber, and a convex lens arranged at one end of the cylinder located inside the incubation chamber.

[0016] As a further optimization scheme of the present invention, the structure of the injection unit includes a gantry frame 2, a plurality of pumping injection cylinders arranged at the top of the gantry frame 2, an injection head connected to the pumping injection cylinder through a delivery pipe, a horizontal plate connecting the injection head, a positioning rod arranged at the lower end of the gantry frame 2 and movably connected to the horizontal plate, two electric guide rail mechanisms arranged in pairs in the length direction of the gantry frame 2, and a horizontal axis arranged on the front side of the delivery pipe and driven to move by the electric guide rail mechanism 2, the bottom end of the positioning rod is provided with a travel switch electrically connected to the electric guide rail mechanism 2, and a boss with a height equal to the height of the porous plate is provided at the position corresponding to the positioning rod on the carrier.

[0017] As a further optimization solution of the present invention, an operating glove is installed on the side of the incubation chamber opposite to the liquid injection unit, and a tool box is slidably provided in the incubation chamber.

[0018] As a second aspect of the present invention, there is also provided a method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos using any of the evaluation devices described above, comprising the following steps:

[0019] Step 1: Select a number of zebrafish embryos with normal development, place them in equal numbers in the holes of a number of multi-well plates, and then place the multi-well plates in a movable oscillating seat on a carrier;

[0020] Step 2: The multi-well plate on the carrier is driven to move to the liquid injection unit by the electric guide mechanism 1, and the cyanobacteria toxin working solution of different concentrations is respectively injected into the multi-well plates by the liquid injection unit, and the zebrafish embryos and the cyanobacteria toxin working solution are evenly mixed by controlling the movable oscillating seat to oscillate;

[0021] Step 3: The multi-hole plate on the carrier is driven to move into the incubation chamber by the electric guide mechanism 1, and the lighting structure is used to provide lighting conditions, so that the zebrafish embryos are incubated in the incubator at the set incubation time;

[0022] Step 4: During the incubation process, the growth status of zebrafish embryos was observed by an observation unit, and the number of deaths, hatchings, and deformities of zebrafish embryos were regularly recorded. The mortality rate, hatching rate, and deformity incidence of zebrafish embryos were used as evaluation indicators to evaluate the developmental toxicity of cyanobacterial toxins on zebrafish embryos.

[0023] The beneficial effects of the present invention are:

[0024] (1) The present invention uses an electric guide rail mechanism to transport a carrier so that fish embryos in a porous plate placed on the carrier are transported to an incubator for incubation. During the transportation process, a liquid injection mechanism automatically injects CYN solutions of different high concentrations into the porous plates on the carrier. During the incubation process, the development of fish embryos in each porous plate in the incubator is observed by the unit, and the mortality rate, hatching rate and deformity rate of the embryos are recorded. The device is easy to use, reduces the intensity of manual labor, improves work efficiency, and facilitates large-scale and efficient evaluation and analysis of the toxicity of high-concentration cyanobacterial toxins on fish embryo development.

[0025] (2) The present invention is provided with a shading unit capable of adjusting the contrast between the bottom of the porous plate and the environment, which is convenient for experimental operators to observe the development of fish embryos and has a good operating effect. In addition, by providing a multi-directional adjustment structure, the observation unit can be further flexibly adjusted according to the use requirements of the experimental operators. Compared with a multi-axial manipulator, the multi-directional adjustment structure has a simple structure, a small footprint, low use and maintenance costs, and a good use effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 A three-dimensional diagram of the overall structure of the device provided by the present invention;

[0027] Figure 2 A schematic diagram of the structure of the object carrier provided by the present invention;

[0028] Figure 3 A schematic diagram of the internal structure of the incubation chamber provided by the present invention;

[0029] Figure 4 A structural stereogram of the shading cloth provided by the present invention;

[0030] Figure 5 A schematic diagram of the overall structure of the liquid injection unit provided by the present invention;

[0031] In the figure: 1, machine base; 2, electric guide rail mechanism 1; 3, load carrier; 31, gantry frame 1; 32, panel; 33, movable oscillating seat; 34, elastic telescopic member; 4, liquid injection unit; 41, gantry frame 2; 42, pumping liquid injection cylinder; 43, liquid injection head; 44, positioning rod; 45, horizontal plate; 46, travel switch; 47, electric guide rail mechanism 2; 48, horizontal axis; 5, incubation chamber; 6, observation unit; 61, observation eye mask; 62, cylinder; 63. Convex lens; 7. Perforated plate; 8. Multi-directional adjustment structure; 81. Annular slide rail; 82. Elastic membrane; 83. Mounting seat; 84. Articulated seat; 85. Telescopic rod; 86. Sliding block; 9. Adjustable shading unit; 91. Active roller; 92. Shading cloth; 93. Winding roller; 10. Drive oscillation unit; 101. Transmission rod; 102. Transmission member; 103. Eccentric connector; 11. Operating gloves; 12. Tool box; 13. Boss. DETAILED DESCRIPTION

[0032] The present application is further described in detail below in conjunction with the accompanying drawings. It is necessary to point out here that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technical personnel in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0033] Example 1

[0034] The present embodiment provides a high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos, which is performed by injecting different high-concentration cyanobacterial toxins into the culture medium of fish embryos, and then incubating the treated fish embryos. The incubation temperature is controlled at 28±1°C, and the light cycle is set to 14 hours of light and 10 hours of darkness. During the set experimental period, the mortality rate, hatching rate and deformity incidence rate of the embryos are regularly recorded, and the developmental toxicity of the cyanobacterial toxins is evaluated based on the statistical data.

[0035] like Figure 1 As shown, the structure of the device includes a base 1, and also includes an electric guide rail mechanism 2 extending along the length direction of the base 1 into the incubation chamber 5. The electric guide rail mechanism 2 is a conventional structure in the field, including a guide rail, a slider, and a motor, etc., which will not be described in detail here.

[0036] A carrier 3 is slidably arranged on an electric guide rail mechanism 2, wherein the carrier 3 is provided with a movable oscillation seat 33 and the movable oscillation seat 33 is provided with a plurality of porous plates 7 for placing fish embryos. In this embodiment, the movable oscillation seat 33 is preferably in two groups, and the power source for driving the movable oscillation seat 33 to move relative to the carrier 3 can be manually pushed by the test operator or can be an electric drive device such as a linear motor installed with the movable oscillation seat 33.

[0037] The fish embryos placed in the multi-well plate 7 are preferably embryos of mature aquatic organism models - zebrafish, preferably a 6-well plate, such as Figure 2 As shown, the shape of the porous plate 7 is designed to match the shape of the movable oscillating seat 33. In addition, the object carrier 3 is provided with an illumination structure at a position below the movable oscillating seat 33, for providing the illumination conditions required for hatching fish embryos.

[0038] An injection unit 4 and an incubation chamber 5 are arranged to extend along the length direction of the base 1. The injection unit 4 is used to inject different high concentrations of cyanobacterial toxin working solutions into a plurality of porous plates 7. A notch is left on the side of the incubation chamber 5 facing the injection unit 4, which is convenient for the electric guide mechanism 2 to push the carrier 3 into the incubation chamber 5. In addition, in order to ensure the structural sealing of the incubation chamber 5, one side of the carrier 3 is designed to have a door plate structure adapted to the notch. An observation unit 6 is arranged on the top of the incubation chamber 5. The observation unit 6 is convenient for the test operator to regularly record the mortality rate, hatching rate and deformity incidence rate of fish embryos during the test. In addition, an operating glove 11 is installed on the side of the incubation chamber 5 relative to the injection unit 4, and a tool box 12 is slidably arranged in the incubation chamber 5. During the experiment, it is necessary to remove dead or deformed embryos while regularly recording to maintain the accuracy of the experiment. The test operator can use the operating glove 11 to use relevant operating tools such as cell clamps to extract and remove the dead embryos, and the tool box 12 can store relevant tools or the extracted and removed dead or deformed embryos.

[0039] The structure of the device also includes an adjustable shading unit 9 arranged between the lighting structure and the porous plate 7, which is used to adjust the contrast between the bottom of the porous plate 7 and the environment, so as to facilitate the observation of the development of the fish embryo through the observation unit 6. The movable oscillating seat 33 is provided with a hollow groove at the position corresponding to the hole of the porous plate 7. The structure of the adjustable shading unit 9 includes an active roller 91, a winding roller 93 and a shading cloth 92 arranged between the active roller 91 and the winding roller 93. The shading cloth 92 has a hollow portion (such as Figure 4 As shown), the non-hollowed-out part of the shading cloth 92 is preferably set to black, because the fish embryo is transparent when it is alive, and the dead embryo is partially or completely milky white. The black background makes it easier to find dead embryos or embryos with abnormal development and deformity. The active roller 91 is driven by an external driving source, preferably a driving motor, to pull the shading cloth 92 so that it is pulled out or rewound relative to the winding roller 93. When the hollowed-out part of the shading cloth 92 corresponds to the hollowed-out groove of the active oscillation seat 33, the lighting structure provides the light required for embryo development. When other parts of the shading cloth 92 rotate to the hollowed-out groove position corresponding to the active oscillation seat 33, at this time, the lower end of the hole position of the porous plate 7 is in a dark state, and the light generated by the lighting structure will overflow from the surrounding position of the shading cloth 92, thereby increasing the light contrast between the lower end of the porous plate 7 and the environment, thereby making it more convenient for the test operator to observe the development of the embryo in the porous plate 7. At the same time, the normal lighting conditions required for the embryo can be guaranteed when not observed. The device is more flexible to use and has good practicality.

[0040] Example 2

[0041] like Figure 2As shown, on the basis of Example 1, in order to further save manpower and ensure the stability of the movable oscillation seat 33 relative to the movable structure of the carrier 3, the carrier 3 includes a gantry 31 and a panel 32 arranged on the gantry 31, elastic telescopic parts 34 arranged at both ends of the movable oscillation seat 33 and movably plugged with the panel 32, and a driving oscillation unit 10 arranged on the gantry 31 for driving the movable oscillation seat 33 to achieve oscillation. Among them, the driving oscillation unit 10 includes a transmission rod 101 and an eccentric connecting member 103 axially connected to the gantry 31, and the transmission rod 101 rotates synchronously with the eccentric connecting member 103 through the transmission member 102. When the eccentric connecting member 103 rotates, its outer end portion intermittently and periodically pushes the movable oscillation seat 33 to achieve oscillation and swinging of the movable oscillation seat 33. The oscillation and swinging of the movable oscillation seat 33 can, on the one hand, make the cyanobacteria toxin working liquid mixed evenly with the embryos and culture medium in the porous plate 7 when the injection unit 4 injects the cyanobacteria toxin working liquid into the porous plate 7; on the other hand, when the experimental operator regularly observes the development of the embryos, it is convenient to change the position of the embryos growing on the wall of the porous plate 7, which is convenient for statistics and recording.

[0042] In specific applications, the power source for driving the transmission rod 101 to rotate is preferably installed on the door panel structure, preferably a driving motor. The transmission rod 101 is connected to the driving source of the driving motor, and rotates under the drive of the driving motor, and drives the eccentric connecting member 103 to rotate through the transmission member 102. The transmission member 102 is preferably a meshing gear set. The eccentric connecting member 103 is preferably a cam member, and its rotation path is a periodic cycle so that its outer end can intermittently and periodically push the movable oscillation seat 33 to produce displacement compared to the panel 32. The displacement of the movable oscillation seat 33 is related to the maximum diameter value of the cam member.

[0043] Example 3

[0044] On the basis of Example 1, in order to facilitate the test operator to use the observation unit 6, as shown in FIG. Figure 3 As shown, the structure of the observation unit 6 includes a cylinder 62 connected to the multi-directional adjustment structure 8, an observation eye mask 61 provided at one end of the cylinder 62 located outside the incubation chamber 5, and a convex lens 63 provided at one end of the cylinder 62 located inside the incubation chamber 5. The convex lens 63 is preferably a combination of an objective lens and an eyepiece, similar to the relevant structure of a microscope, and its purpose is to magnify the morphology of the embryos in the multi-well plate 7.

[0045] In specific applications, the eyes of the experimental operator can be fitted with the position of the observation eye mask 61, and the multi-hole plate 7 on the carrier 3 in the incubation chamber 5 can be magnified through the convex lens 63 so that the morphology of the embryos in each hole of the multi-hole plate 7 can be clearly displayed. In order to facilitate the flexible adjustment of the observation unit 6 according to the usage requirements of the experimental operator, in this embodiment, a multi-directional adjustment structure 8 is further provided. The structure of the multi-directional adjustment structure 8 includes an elastic membrane 82 arranged at the top of the incubation chamber 5. The elastic membrane 82 is set to an opaque material to meet the requirements of dark embryo culture, and has good elasticity to meet the movement of the observation unit 6. A mounting seat 83 is arranged on the elastic membrane 82 to fix the observation unit 6, an annular slide rail 81 arranged in the incubation chamber 5, a sliding block 86 movably arranged in the annular slide rail 81, a telescopic rod 85 with one end hinged to the outside of the sliding block 86, and an articulated seat 84 hinged to the other end of the telescopic rod 85, wherein the articulated seat 84 is hinged to one end of the observation unit 6 extending into the incubation chamber 5, and the telescopic rod 85 is preferably two, and the sliding block 86 is preferably a spherical slider. The track shape of the annular slide rail 81 matches it. When the observation unit 6 is not in use, the telescopic rod 85 and its two The cooperation between the sliding block 86 and the hinged seat 84 at the end can realize the structural support of the observation unit 6 and avoid the elastic membrane 82 from being subjected to stress, thereby ensuring the service life of the elastic membrane 82. The telescopic rod 85 can also meet the support requirements of the observation unit 6 when it moves. Specifically, when the observation unit 6 needs to move horizontally, the displacement requirement of the observation unit 6 can be achieved through the extension and contraction of the telescopic rod 85 and the sliding block 86 connected to the telescopic rod 85 sliding on the annular slide rail 81. When the observation unit 6 needs to move vertically, it can be achieved through the rotation of one end of the telescopic rod 85 and the sliding block 86, and the other end and the observation unit 6. It has high operational flexibility. Compared with a multi-axial manipulator, the multi-directional adjustment structure 8 provided in this embodiment has a simple structure, a small footprint, low use and maintenance costs, and a good use effect.

[0046] Example 4

[0047] Based on Example 1, this example provides a new structure of a liquid injection unit 4, such as Figure 1 , 5As shown, the structure of the injection unit 4 includes a gantry 41, a plurality of pumping injection cylinders 42 are arranged at the top of the gantry 41, an injection head 43 connected to the pumping injection cylinder 42 through a delivery pipe, a horizontal plate 45 connected to the injection head 43, a positioning rod 44 arranged at the lower end of the gantry 41 and movably connected to the horizontal plate 45, an electric guide mechanism 47 arranged in pairs on the length direction of the gantry 41, and a horizontal axis 48 arranged on the front side of the delivery pipe and driven to move by the electric guide mechanism 47. The number of pumping injection cylinders 42 is set according to the concentration gradient of the cyanobacteria toxin working solution required to be set during the test, and each pumping injection cylinder 42 stores the cyanobacteria toxin working solution with different high concentrations. The bottom end of the positioning rod 44 is provided with a travel switch 46 electrically connected to the electric guide mechanism 47, and a boss 13 with a height equal to the placement height of the porous plate 7 is provided at the position corresponding to the positioning rod 44 on the carrier 3.

[0048] In specific application, when the electric guide mechanism 1 2 drives the carrier 3 to move to the injection unit 4, the boss 13 on the carrier 3 contacts the travel switch 46 at the lower end of the positioning rod 44. After the travel switch 46 is actuated, the electric guide mechanism 2 47 and the pumping injection cylinder 42 are started. The injection amount of the pumping injection cylinder 42 is set by the control device of the device. The electric guide mechanism 2 47 controls the reciprocating distance of the horizontal axis 48 according to the reciprocating height of the injection head 43 and the spacing between the adjacent holes of the porous plate 7. It is designed and set by the control device of the device. The electric guide mechanism 2 47 controls the horizontal axis 48 to move back and forth. During the reciprocating movement of 48, the horizontal axis 48 contacts the delivery tube and drives the delivery tube to pull the connected injection head 43 and the horizontal plate 45 to realize lifting and lowering displacement under the limit of the positioning rod 44, and at the same time cooperates with the work of the pumping injection cylinder 42 to realize continuous injection operation. The equipment has good maneuverability. When the carrier 3 moves to the point where the boss 13 does not contact the lower end of the positioning rod 44, the electric guide rail mechanism 2 47 and the pumping injection cylinder 42 are immediately closed. The equipment has good maneuverability and can simultaneously realize the operation of injecting different high-concentration cyanobacteria toxin working solutions into the corresponding porous plates 7. Compared with the manual addition method, it saves time and improves efficiency.

[0049] Example 5

[0050] Based on Example 1, this embodiment further provides a method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos using any of the evaluation devices described above, comprising the following steps:

[0051] Step 1: Select a number of zebrafish embryos with normal development, place them in equal numbers in the holes of a number of porous plates 7, and then place the plurality of porous plates 7 in the movable oscillating seats 33 on the carrier 3;

[0052] Step 2: The multi-porous plate 7 on the carrier 3 is driven to move to the liquid injection unit 4 by the electric guide rail mechanism 1, and the liquid injection unit 4 injects equal volumes of cyanobacteria toxin working solutions of different concentrations into the multi-porous plates 7, and controls the movable oscillating seat 33 to oscillate so that the zebrafish embryos and the cyanobacteria toxin working solutions are evenly mixed;

[0053] Step 3: The multi-hole plate 7 on the carrier 3 is driven to move into the incubation chamber 5 by the electric guide rail mechanism 1 2, and the lighting structure is used to provide lighting conditions, so that the zebrafish embryos are incubated in the incubator 5 at the set incubation time;

[0054] Step 4: During the incubation process, the growth status of the zebrafish embryos is observed by the observation unit 6, and the number of deaths, hatching numbers and deformities of the zebrafish embryos are regularly recorded. The mortality rate, hatching rate and deformity incidence rate of the zebrafish embryos are used as evaluation indicators to evaluate the developmental toxicity of cyanobacterial toxins on the zebrafish embryos.

[0055] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A device for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos, comprising a base (1), characterized in that: It also includes an electric guide rail mechanism (2) extending along the length direction of the base (1) into the incubation chamber (5); A carrier (3) is slidably arranged on the electric guide rail mechanism (2), the carrier (3) is provided with a movable oscillating seat (33), and the movable oscillating seat (33) is provided with a plurality of porous plates (7) for placing fish embryos, and the carrier (3) is provided with a lighting structure at a position below the movable oscillating seat (33) for providing the required lighting conditions for the fish embryos; A liquid injection unit (4) and an incubation chamber (5) are arranged extending along the length direction of the machine base (1), wherein the liquid injection unit (4) is used to inject cyanobacteria toxin working solutions of different high concentrations into a plurality of porous plates (7), and an observation unit (6) is arranged on the top of the incubation chamber (5); A shading adjustment unit (9) is provided between the lighting structure and the porous plate (7) and is used to adjust the contrast between the bottom of the porous plate (7) and the environment, so as to facilitate observation of the development of the fish embryos through the observation unit (6).

2. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The object carrier (3) comprises a gantry frame (31), a panel (32) arranged on the gantry frame (31), elastic telescopic parts (34) arranged at both ends of a movable oscillation seat (33) and movably connected to the panel (32), and a driving oscillation unit (10) arranged on the gantry frame (31) and used for driving the movable oscillation seat to oscillate.

3. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 2 is characterized by: The driving oscillation unit (10) comprises a transmission rod (101) and an eccentric connecting member (103) axially connected to a gantry frame (31); the transmission rod (101) rotates synchronously with the eccentric connecting member (103) via a transmission member (102); when the eccentric connecting member (103) rotates, the outer end of the eccentric connecting member (103) intermittently and periodically pushes the movable oscillation seat (33) to achieve oscillation and swing of the movable oscillation seat (33).

4. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: A hollow groove is provided on the movable oscillating seat (33) at a position corresponding to the hole of the porous plate (7); the structure of the adjustable shading unit (9) comprises an active roller (91), a winding roller (93) and a shading cloth (92) arranged between the active roller (91) and the winding roller (93); and the shading cloth (92) has a hollow portion.

5. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The top of the incubation chamber (5) is provided with a multi-directional adjustment structure (8) mounted on the observation unit (6), the structure of the multi-directional adjustment structure (8) comprising an elastic membrane (82) arranged at the top of the incubation chamber (5), a mounting seat (83) arranged on the elastic membrane (82) to fix the observation unit (6), an annular slide rail (81) arranged in the incubation chamber (5), a sliding block (86) movably arranged in the annular slide rail (81), a telescopic rod (85) with one end hinged to the outer side of the sliding block (86), and an articulated seat (84) hinged to the other end of the telescopic rod (85), the articulated seat (84) being articulated to one end of the observation unit (6) extending into the incubation chamber (5).

6. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The structure of the observation unit (6) comprises a cylinder (62) connected to the multi-directional adjustment structure (8), an observation eye mask (61) arranged at one end of the cylinder (62) located outside the incubation chamber (5), and a convex lens (63) arranged at one end of the cylinder (62) located inside the incubation chamber (5).

7. The high-concentration cyanobacterial toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: The structure of the liquid injection unit (4) comprises a gantry frame (41), a plurality of pumping liquid injection cylinders (42) arranged at the top of the gantry frame (41), a liquid injection head (43) connected to the pumping liquid injection cylinder (42) through a delivery pipe, a horizontal plate (45) connected to the liquid injection head (43), a positioning rod (44) arranged at the lower end of the gantry frame (41) and movably sleeved with the horizontal plate (45), two electric guide rail mechanisms (47) arranged in pairs in the length direction of the gantry frame (41), and a horizontal shaft (48) arranged at the front side of the delivery pipe and driven to move by the electric guide rail mechanism (47), a travel switch (46) electrically connected to the electric guide rail mechanism (47) is arranged at the bottom end of the positioning rod (44), and a boss (13) whose height is equal to the placement height of the porous plate (7) is arranged at a position corresponding to the positioning rod (44) on the carrier (3).

8. The high-concentration cyanobacteria toxin developmental toxicity evaluation device based on fish embryos according to claim 1, characterized in that: An operating glove (11) is installed on the side of the incubation chamber (5) opposite to the liquid injection unit (4), and a tool box (12) is slidably arranged in the incubation chamber (5).

9. A method for evaluating the developmental toxicity of high-concentration cyanobacterial toxins based on fish embryos using the device according to any one of claims 1 to 8, characterized in that: The following steps are involved: Step 1: Select a number of zebrafish embryos with normal development, place an equal number of them in the holes of a number of porous plates (7), and then place the number of porous plates (7) in a movable oscillating seat (33) on the carrier (3); Step 2: The multi-hole plate (7) on the carrier (3) is driven to move to the liquid injection unit (4) by the electric guide rail mechanism 1 (2), and the same volume of cyanobacteria toxin working solution of different concentrations is respectively injected into a plurality of multi-hole plates (7) by the liquid injection unit (4), and the zebrafish embryos and the cyanobacteria toxin working solution are uniformly mixed by controlling the movable oscillating seat (33) to oscillate; Step 3: driving the multi-hole plate (7) on the carrier (3) to move into the incubation chamber (5) through the electric guide rail mechanism 1 (2), and providing lighting conditions through the lighting structure to incubate the zebrafish embryos in the incubator (5) at a set incubation time; Step 4: During the incubation process, the growth status of the zebrafish embryos is observed by an observation unit (6), and the number of deaths, hatchings and deformities of the zebrafish embryos are regularly recorded. The mortality rate, hatching rate and deformity incidence rate of the zebrafish embryos are used as evaluation indicators to evaluate the developmental toxicity of cyanobacterial toxins on the zebrafish embryos.

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