Organ-like tissue crushing device for test tube and sample temperature control accessory thereof

Abstract

The invention provides an organ-like tissue crushing device for a test tube, and relates to the technical field of experimental apparatuses, a protection mechanism, a crushing mechanism, a height locking mechanism and a liquid taking mechanism, the crushing mechanism and the height locking mechanism are both mounted in the protection mechanism, the height locking mechanism can push the crushing mechanism to move, and the liquid taking mechanism can take liquid from the crushing mechanism. The protection mechanism is arranged at one end of the crushing mechanism, the end of the crushing mechanism extends out of the interior of the protection mechanism and is limited, the liquid taking mechanism is arranged at the other end of the protection mechanism, the end of the liquid taking mechanism is connected with the end of the crushing mechanism in an inserted mode, the crushing mechanism comprises a variable frequency motor, a storage battery, a transmission rod and a crushing tool bit, and the variable frequency motor is electrically connected with the storage battery. When in use, the variable frequency motor and the storage battery are respectively arranged in the pen-shaped protective shell to drive the transmission rod to drive the crushing tool bit to rotate to crush tissue cells, the integration degree is high, the device has a smaller size, the crushing operation can be directly performed in a test tube, and the effect of crushing a small amount of tissues is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental equipment, and in particular to an organoid tissue fragmentation device for test tubes. Background Art

[0002] One of the key advances in stem cell research over the past decade has been the development of organoid systems. This in vitro culture system consists of a self-renewing stem cell population that can differentiate into multiple organ-specific cell types, have a similar spatial organization to the corresponding organ, and can reproduce some of the functions of the corresponding organ, thus providing a highly physiologically relevant system. Organoids can be generated from tissue samples containing adult stem cells, single adult stem cells, or through the directed differentiation of pluripotent stem cells. Because some organoid model systems are characterized by the presence of an active stem cell population, organoids can be greatly expanded. For example, in 5 to 6 weeks, a single progenitor cell can produce up to 1x 10 ^6 The new study, published in the journal Nature, provides researchers with a highly reliable and scalable platform to study a variety of organs.

[0003] When observing and analyzing tissue samples, the tissue samples need to be broken for better observation. Existing tissue breaking devices are generally large in size, and cannot or are not convenient for breaking tissue samples in a test tube, and a small amount of tissue samples cannot be broken at all.

[0004] To this end, the present application proposes an organoid tissue fragmentation device for test tubes, which is used to fragment tissue samples in test tubes. Summary of the invention

[0005] In view of the above-mentioned shortcomings of the prior art, an object of the present invention is to provide an organoid tissue fragmentation device for test tube use, so as to solve the problems in the prior art.

[0006] To achieve the above-mentioned and other related purposes, the present invention provides a test tube organoid tissue fragmentation device, comprising: a protection mechanism, a fragmentation mechanism, a height locking mechanism and a liquid collection mechanism;

[0007] The crushing mechanism and the height locking mechanism are both installed inside the protection mechanism, and the height locking mechanism can push the crushing mechanism to move so that the end of the crushing mechanism extends out from the inside of the protection mechanism and is limited;

[0008] The liquid taking mechanism is arranged at the other end of the protection mechanism, and the end of the liquid taking mechanism is plugged into the end of the crushing mechanism;

[0009] The crushing mechanism includes a variable frequency motor, a battery, a transmission rod and a crushing cutter head, the variable frequency motor and the battery are electrically connected, the transmission rod is connected to the rotor of the variable frequency motor, and the two ends of the transmission rod are respectively located at the mouths of the two ends of the protection mechanism;

[0010] The locking mechanism includes a reset elastic member and a locking component, the locking component can drive the variable frequency motor and the battery to move, so that the crushing cutter head can be extended and retracted, and the axis of the locking component is penetrated by the transmission rod;

[0011] The resetting elastic member is sleeved on the outer surface of the transmission rod, and the two ends of the resetting elastic member are respectively overlapped with the end of the variable frequency motor and the inner wall of the protection mechanism.

[0012] Preferably, the crushing cutter head is detachably mounted on the end of the transmission rod, and the axes of the transmission rod and the crushing cutter head are both hollow;

[0013] The battery is provided with a frequency conversion switch and a charging port respectively, and both the frequency conversion switch and the charging port extend to the outer surface of the protection mechanism, and the frequency conversion switch can control the power supply and working frequency of the frequency conversion motor.

[0014] Preferably, the protection mechanism comprises a protection shell, and a telescopic slide groove is provided on the outer surface of the protection shell;

[0015] The locking component includes: a rotating base, a fixed limiting cylinder and a pressure cylinder, wherein the rotating base, the fixed limiting cylinder and the pressure cylinder are sleeved with each other, and a hand-pushing block is arranged on the outer surface of the pressure cylinder, and the hand-pushing block extends to the outer surface of the protection mechanism;

[0016] The frequency conversion switch, the charging port and the push block all pass through the telescopic slide slot and can slide in the telescopic slide slot.

[0017] Preferably, a support step and a limit groove are provided inside the protective shell, a limit slider is provided on the outer surface of the variable frequency motor, the limit slider is slidably connected to the limit groove, and one end of the reset elastic member overlaps the support step.

[0018] Preferably, a clamping piece is provided on the outer surface of the protective shell, and the clamping piece is elastic.

[0019] Preferably, the liquid extraction mechanism includes a negative pressure chamber, a connecting head, a piston member and a rebound member, wherein the connecting head is fixed at one end of the negative pressure chamber and communicates with the interior of the negative pressure chamber, the piston member extends into the interior of the negative pressure chamber, and the piston member can slide inside the negative pressure chamber and compress the air, and the rebound member is located inside the negative pressure chamber and can apply a rebound force to the piston member.

[0020] Preferably, a sleeve groove is provided inside the connector, the end of the transmission rod can be inserted into the sleeve groove, and the upper end of the transmission rod can slide axially along the inside of the sleeve groove.

[0021] Preferably, the piston member comprises a piston end and a push handle, the piston end is located inside the negative pressure chamber, and the push handle extends to the outer surface of the negative pressure chamber;

[0022] The push handle is provided with a damping hole and a scale mark, respectively, and the damping hole and the scale mark are distributed along the extension direction of the push handle;

[0023] A damping member is disposed inside the negative pressure chamber, and the damping member cooperates with the damping hole to indicate the movement stroke of the piston member.

[0024] Preferably, the damping member includes a damping telescopic cylinder, a damping spring and a damping rod, the damping spring is installed inside the damping telescopic cylinder to apply pressure to one end of the damping rod, the other end of the damping rod passes through the outside of the damping telescopic cylinder, and the damping telescopic cylinder can be plugged into the damping hole.

[0025] A sample tissue temperature control accessory for a test tube, the temperature control accessory can be used together with a crushing device, the temperature control accessory comprises a support base and a test tube rack, the test tube rack is arranged above the support base, and a plurality of test tube holes are arranged on the top of the test tube rack for fixing the test tube;

[0026] A heating plate is arranged between the support base and the test tube rack, a power supply is arranged inside the support base, and the power supply can supply power to the heating plate, a liquid cavity is arranged inside the test tube rack, a heat conducting plate is arranged at the bottom of the liquid cavity, and the heating plate and the heat conducting plate are in conflict with each other;

[0027] The interior of the liquid chamber is filled with liquid.

[0028] Preferably, a control component, a display screen and a quick connector are respectively provided on the support base, the control component is electrically connected to the heating plate, and the quick connector is electrically connected to the power supply;

[0029] The control components, display screen and quick connectors are all waterproofed.

[0030] As described above, the in vitro organoid tissue fragmentation device of the present invention has the following beneficial effects:

[0031] 1. The present invention respectively arranges a variable frequency motor and a battery inside a pen-shaped protective shell to drive a transmission rod to drive a crushing cutter head to rotate and crush tissue cells. It has a high degree of integration and a small size. The crushing operation can be directly performed in a test tube, achieving the effect of crushing a small amount of tissue.

[0032] 2. The present invention applies rebound pressure to the frequency conversion motor by arranging a reset elastic member inside the protective shell, and arranges a rotating base, a fixed limiting cylinder and a pressure cylinder inside the protective shell to cooperate and limit the position of the frequency conversion motor, so that the crushing cutter head can be extended and retracted inside the protective shell, thereby ensuring that the crushing cutter head remains clean and safe when not in use.

[0033] 3. The present invention installs a negative pressure chamber at the end of the protective shell, connects the connector at the end of the negative pressure chamber to the transmission rod, and installs a slidable piston inside the negative pressure chamber for extracting and injecting liquid, thereby realizing convenient addition of tissue fluid when crushing sample tissue.

[0034] 4. The present invention arranges damping holes and scale marks on the surface of the piston, and cooperates with the damping part and the damping hole to provide stroke prompts. Therefore, when extracting or injecting tissue fluid, the measurement can be judged according to the scale marks, and the measurement can be determined by the prompt sound and resistance of the damping part, thereby achieving the effect of precise control of measurement.

[0035] 5. The present invention cooperates with a support base and a test tube rack, and a heating plate is arranged on the support base to heat the liquid inside the liquid cavity, thereby heating and keeping the sample tissue warm. When storing the sample, it can be placed in a low-temperature storage. The liquid in the liquid cavity can keep the sample at a low temperature for a certain period of time, thereby achieving low-temperature storage of the sample tissue and heating to enhance cell activity.

[0036] Therefore, the present invention effectively overcomes various shortcomings in the prior art and has high industrial utilization value. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 Shown is a schematic structural diagram of the crushing device of the present invention.

[0038] Figure 2 Shown is a structural cross-sectional view of the crushing device of the present invention.

[0039] Figure 3 Shown is a schematic structural diagram of the height locking mechanism of the crushing device of the present invention.

[0040] Figure 4 It shows a schematic diagram of the connection between the crushing mechanism and the liquid extraction mechanism of the crushing device of the present invention.

[0041] Figure 5 Shown is a schematic diagram of the structure of the liquid extraction mechanism of the crushing device of the present invention.

[0042] Figure 6 Shown is a structural cross-sectional view of the liquid extraction mechanism of the crushing device of the present invention.

[0043] Figure 7 Shown as the present invention Figure 5 A schematic diagram of the enlarged structure at point A in the middle.

[0044] Figure 8 Shown is a schematic structural diagram of the temperature control accessory of the present invention.

[0045] Fig. 9 Shown is a schematic diagram of the structural assembly of the temperature control accessory of the present invention.

[0046] Fig.10 Shown is a cross-sectional view of the test tube rack structure of the temperature control accessory of the present invention.

[0047] Component number description

[0048] 101, protective shell; 102, telescopic slide; 103, supporting step; 104, limiting groove; 105, clamping piece; 201, frequency conversion motor; 202, battery; 203, transmission rod; 204, crushing cutter head; 205, frequency conversion switch; 206, charging port; 207, limiting slider; 301, reset elastic member; 302, rotating base; 303, fixed limiting cylinder; 304, pressure cylinder; 305, hand push block; 401, negative pressure chamber; 402, Connector; 4021, sleeve groove; 403, piston member; 4031, damping hole; 4032, scale mark; 404, rebound member; 405, damping member; 4051, damping telescopic cylinder; 4052, damping spring; 4053, damping rod; 501, support base; 502, test tube rack; 503, test tube hole; 504, heating plate; 505, liquid cavity; 506, heat conducting plate; 507, control component; 508, display screen; 509, quick connector. DETAILED DESCRIPTION

[0049] The following is a description of the implementation of the present invention by means of specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0050] See also Figures 1 to 10. It should be noted that the structures, proportions, sizes, etc. illustrated in the drawings in this specification are only used to match the contents disclosed in the specification for people familiar with this technology to understand and read, and are not used to limit the limiting conditions for the implementation of the present invention, so they have no substantial technical significance. Any modification of the structure, change in the proportional relationship, or adjustment of the size should still fall within the scope of the technical content disclosed by the present invention without affecting the effects and purposes that can be achieved by the present invention. At the same time, the terms such as "upper", "lower", "left", "right", "middle" and "one" quoted in this specification are only for the convenience of description, and are not used to limit the scope of the implementation of the present invention. Changes or adjustments in their relative relationships should also be regarded as the scope of the implementation of the present invention without substantially changing the technical content.

[0051] like Figure 1-Figure 7 As shown, the present invention provides an organoid tissue fragmentation device for a test tube, comprising: a protection mechanism, a fragmentation mechanism, a height locking mechanism and a liquid extraction mechanism. The fragmentation mechanism and the height locking mechanism are both installed inside the protection mechanism. The height locking mechanism can push the fragmentation mechanism to move, so that the end of the fragmentation mechanism extends from the inside of the protection mechanism and is limited, so that the end of the fragmentation mechanism can extend to the inside of the test tube. When the fragmentation function is not in use, the end of the fragmentation mechanism is prevented from being contaminated and causing harm to the operator. In order to further improve safety and cleanliness, a detachable cap can be configured on the protection mechanism to seal the end of the protection mechanism.

[0052] The liquid collection mechanism is arranged at the other end of the protection mechanism, so that tissue fluid can be added at any time during the sample tissue crushing process, which improves the convenience of operation. The end of the liquid collection mechanism is plugged into the end of the crushing mechanism to improve the sealing of the connection. When the crushing mechanism is extended or retracted, the crushing mechanism and the liquid collection mechanism are always connected. And when the axis of the crushing mechanism rotates, the connection does not separate.

[0053] The crushing mechanism includes a variable frequency motor 201, a battery 202, a transmission rod 203 and a crushing blade 204. The variable frequency motor 201 and the battery 202 are electrically connected, and the variable frequency motor 201 is powered by the battery 202. The transmission rod 203 is connected to the rotor of the variable frequency motor 201. When the variable frequency motor 201 is powered on, the transmission rod 203 rotates with the rotation of the motor under the action of magnetic force. The two ends of the transmission rod 203 are respectively located at the mouths of the two ends of the protective mechanism, one end is used to install the blade, and the other end is used to connect with the liquid extraction mechanism; in order to improve stability, the battery 202 is columnar, and the outer surface of the battery 202 is in contact with the inner wall of the protective shell 101. A through hole is set in the middle of the battery 202 to allow the transmission rod 203 to pass through.

[0054] The crushing cutter head 204 is installed at the end of the transmission rod 203, and the crushing cutter head 204 is detachable and replaceable. When the diameter and length of the crushing cutter head 204 do not match the test tube, the crushing cutter head 204 of different diameters and / or lengths can be replaced to adapt to different types of test tubes to improve adaptability. The axes of the transmission rod 203 and the crushing cutter head 204 are both hollow, so that when the liquid extraction mechanism extracts liquid, the liquid can be sucked from the crushing cutter head 204 or the transmission rod 203 into the interior of the transmission rod 203 for storage. A small amount of liquid will be stored in the interior of the transmission rod 203 and will not enter the interior of the liquid extraction mechanism to avoid contaminating the interior of the liquid extraction mechanism. When cleaning, it is only necessary to repeatedly draw pure water or disinfectant to rinse the cavity in the transmission rod 203.

[0055] The battery 202 is provided with a frequency conversion switch 205 and a charging port 206. The frequency conversion switch 205 is used to control the power supply of the battery 202 to the frequency conversion motor 201. The frequency conversion switch 205 can control the power supply and working frequency of the frequency conversion motor 201 to achieve an adjustable crushing rate. The charging port 206 is used to connect a power cord to charge the battery 202. Both the frequency conversion switch 205 and the charging port 206 extend to the outer surface of the protection mechanism and move with the movement of the battery 202.

[0056] The height locking mechanism includes a reset elastic member 301 and a locking component. The locking component includes: a rotating base 302, a fixed limiting cylinder 303 and a pressure cylinder 304. The rotating base 302, the fixed limiting cylinder 303 and the pressure cylinder 304 are mutually connected to form a structure such as the internal locking refill height of a ballpoint pen, which is used to lock the height of the variable frequency motor 201 in the application, so that the crushing cutter head 204 can be stably located outside the protective mechanism and contact the sample tissue in the test tube. The outer surface of the pressure cylinder 304 is provided with a hand push block 305, which extends to the outer surface of the protective mechanism. The hand push block 305 is used to facilitate the finger to push the pressure cylinder 304 to apply pressure to the rotating base 302, so that the rotating base 302 rotates under the pressure of the inclined teeth, and cooperates with the fixed limiting cylinder 303 to realize the positioning function, and the fixed limiting cylinder 303 is fixed to the inner wall of the protective mechanism. Pushing the hand push block 305 can drive the variable frequency motor 201 and the battery 202 to move so that the crushing cutter head 204 can be extended and retracted. The axes of the rotating base 302, the fixed limit cylinder 303 and the pressure cylinder 304 are all penetrated by the transmission rod 203, thereby avoiding interference with the rotation of the crushing cutter head 204 and allowing the crushing cutter head 204 to be connected to the liquid extraction mechanism.

[0057] The resetting elastic member 301 is sleeved on the outer surface of the transmission rod 203, and the two ends of the resetting elastic member 301 overlap the end of the variable frequency motor 201 and the inner wall of the protection mechanism respectively. Therefore, when the variable frequency motor 201 loses the limit constraint, the resetting elastic member 301 will push the variable frequency motor 201 back to the initial position to realize the recovery of the crushing cutter head 204, thereby improving the safety and cleanliness of the crushing cutter head 204.

[0058] In some embodiments, the protection mechanism of the present invention includes a protective shell 101, and the overall shape of the protective shell 101 is similar to a ballpoint pen, so as to facilitate carrying and operation. A telescopic slide 102 is provided on the outer surface of the protective shell 101. The frequency conversion switch 205, the charging port 206 and the hand push block 305 all pass through the telescopic slide 102, and can slide in the telescopic slide 102, so as to avoid motion interference caused by the movement of the battery 202 and the pressure cylinder 304, and to limit the angle of the battery 202 and the pressure cylinder 304.

[0059] In some embodiments, a support step 103 and a limit groove 104 are provided inside the protective shell 101 of the present invention. A limit slider 207 is provided on the outer surface of the variable frequency motor 201. The limit slider 207 is slidably connected to the limit groove 104, thereby limiting the installation angle of the variable frequency motor 201 to prevent the variable frequency motor 201 from rotating when the rotor rotates. One end of the reset elastic member 301 overlaps the support step 103, which is used to support the reset elastic member 301, so that the reset elastic member 301 can be stably arranged inside the protective shell 101, and the reset elastic member 301 is prevented from being twisted and deformed due to uneven force.

[0060] In some embodiments, the outer surface of the protective shell 101 of the present invention is provided with a clamping member 105, and the clamping member 105 is elastic. When carrying, the clamping member 105 can be clamped in a clothes pocket by its own elasticity, so as to achieve the purpose of convenient carrying and not easy to lose.

[0061] In some embodiments, the liquid extraction mechanism of the present invention includes a negative pressure chamber 401, a connector 402, a piston member 403, and a rebound member 404. The connector 402 is fixed to one end of the negative pressure chamber 401 and is connected to the interior of the negative pressure chamber 401. The piston member 403 extends to the interior of the negative pressure chamber 401, and the piston member 403 can slide inside the negative pressure chamber 401 and compress the air to extract tissue fluid from the container through the negative pressure principle. The rebound member 404 is located inside the negative pressure chamber 401 and can apply a rebound force to the piston member 403, so that the piston member 403 can easily return to the initial position to facilitate the extraction and injection of tissue fluid.

[0062] In some embodiments, the connector 402 of the present invention is provided with a sleeve groove 4021 inside, and the end of the transmission rod 203 can be inserted into the sleeve groove 4021, so that the sealing of the connection is improved by the insertion of the sleeve groove 4021 and the transmission rod 203. The upper end of the transmission rod 203 can slide along the inner axial direction of the sleeve groove 4021, and when the end of the transmission rod 203 is displaced, the sleeve groove 4021 and the transmission rod 203 always maintain a plug-in relationship. At the same time, the connection between the sleeve groove 4021 and the transmission rod 203 remains smooth to avoid affecting the rotation of the transmission rod 203.

[0063] In some embodiments, the piston member 403 of the present invention includes a piston end and a push handle, wherein the piston end is located inside the negative pressure chamber 401, and the push handle extends to the outer surface of the negative pressure chamber 401. The piston end is completely in contact with the inner wall of the negative pressure chamber 401 to achieve sealing, and the push handle is used to facilitate the movement of the piston end in the negative pressure chamber 401 by driving the piston end with a finger.

[0064] The push handle is provided with a damping hole 4031 and a scale mark 4032, and the damping hole 4031 and the scale mark 4032 are distributed along the extension direction of the push handle. A damping member 405 is provided inside the negative pressure chamber 401, and the damping member 405 cooperates with the damping hole 4031 to prompt the movement stroke of the piston member 403. Specifically, the damping hole 4031 is set at an interval of 5ml, 10ml or a customized interval. Each time the damping hole 4031 contacts the damping member 405, the end of the damping member 405 is inserted into the inside of the damping hole 4031 to form resistance, which is used to remind that a specified interval is formed once, and only by applying greater pressure can the damping hole 4031 be crossed to continue pushing. At this time, the role of the rebound member 404 is to avoid providing pressure when increasing the force, so as to avoid excessive movement of the piston member 403. Taking the 5 ml interval as an example, when the piston 403 is pressed, after two resistances are encountered, it can be clearly known that 10 ml of tissue fluid has been extracted or injected, and so on. There is no need to deliberately observe the scale of the scale mark 4032, which improves the convenience of use.

[0065] In some embodiments, the damping member 405 of the present invention includes a damping telescopic cylinder 4051, a damping spring 4052, and a damping rod 4053. The damping spring 4052 is installed inside the damping telescopic cylinder 4051 to apply pressure to one end of the damping rod 4053, and the other end of the damping rod 4053 penetrates the outside of the damping telescopic cylinder 4051, and the damping telescopic cylinder 4051 can be plugged into the damping hole 4031. The damping spring 4052 can make the damping rod 4053 always apply pressure to the outer surface of the push handle of the piston member 403, and make the damping rod 4053 inserted into the damping hole 4031. The moment the damping rod 4053 is inserted into the damping hole 4031, a mechanical impact sound is generated to enhance the prompt effect. The end of the damping rod 4053 and the mouth of the damping hole 4031 are both provided with chamfers, so that when the damping rod 4053 is subjected to greater pressure, it can be retracted to the inside of the damping telescopic cylinder 4051 and cross the damping hole 4031.

[0066] like Figure 8-Figure 10 As shown, a sample tissue temperature control accessory for a test tube includes a support base 501 and a test tube rack 502. The test tube rack 502 is arranged above the support base 501. A plurality of test tube holes 503 are arranged on the top of the test tube rack 502 for fixing the test tube.

[0067] A heating plate 504 is arranged between the support base 501 and the test tube rack 502, and a power supply is arranged inside the support base 501, and the power supply can supply power to the heating plate 504, so that the heating plate 504 generates heat. The heating plate 504 is a PTC semiconductor heating component, which is used to achieve controllable heating, so that the heating temperature of the heating plate 504 can be controlled as needed. A liquid cavity 505 is arranged inside the test tube rack 502, and the liquid cavity 505 is filled with liquid. Therefore, when the heating plate 504 is heated, the liquid will be heated first, and the liquid will evenly diffuse the heat and heat the test tube inserted in the test tube hole 503, so as to avoid uneven heating of the test tube. At the same time, the outer surface of the test tube rack 502 is a heat-insulating material, which is used to keep the liquid warm and reduce the heat dissipation of the liquid. A heat-conducting plate 506 is arranged at the bottom of the liquid cavity 505, and the heating plate 504 and the heat-conducting plate 506 are in conflict with each other. It is used to improve the efficiency of heat conduction from the heating plate 504 to the inside of the liquid cavity 505. The temperature during heating is generally controlled below 25 degrees to avoid inactivation due to excessive temperature. When the sample needs to be stored, the entire temperature control accessory can be placed in a refrigerator for low-temperature storage. After the temperature control accessory is removed, the liquid in the liquid cavity 505 has poor thermal conductivity, so the sample will be kept at a low temperature for a period of time, just like the effect of a laboratory ice tray.

[0068] In some embodiments, a control component 507, a display screen 508 and a quick connector 509 are respectively provided on the support base 501 of the present invention. The control component 507 is electrically connected to the heating plate 504, and the output power of the heating plate 504 is controlled by the control component 507, so as to realize the controllability of the heating temperature. The quick connector 509 is electrically connected to the power supply, and is used to charge and / or supply power to the power supply installed inside the support base 501 through a power cord. The power supply is generally a lithium battery, and can also be a power module. The display screen 508 is used to display the temperature set by the control component 507, and to display the temperature of the liquid inside the liquid cavity 505. In order to monitor the internal temperature of the liquid cavity 505, a temperature sensor is provided inside the liquid cavity 505.

[0069] The control component 507, the display screen 508 and the quick connector 509 are all waterproofed to prevent condensed water from entering the interior of the support base 501 through the gaps between the control component 507, the display screen 508 and the quick connector 509 when the temperature control accessories are placed in the low-temperature storage, thereby causing a power short circuit.

[0070] The specific use process of the present invention is as follows:

[0071] The crushing blade 204 is extended to the inside of the protective housing 101 by pushing the hand push block 305;

[0072] Press the piston 403 and withdraw the piston 403 to extract an appropriate amount of tissue fluid under the action of negative pressure for later use;

[0073] Insert the test tube with the sample tissue into the test tube hole 503 of the temperature control component to fix the test tube;

[0074] Extending the crushing blade 204 to the inside of the test tube to contact the sample tissue;

[0075] The variable frequency motor 201 is started to drive the transmission rod 203 to rotate at high speed, and the sample tissue is crushed by the crushing blade 204;

[0076] Press the piston 403 appropriately at any time to inject tissue fluid into the test tube, so that the sample tissue remains fluid when it is broken;

[0077] According to different requirements of sample tissue, the heating plate 504 is activated as needed to heat the test tube or a temperature control accessory for low-temperature preservation is used to increase or decrease the activity of the sample tissue.

[0078] In summary, the in vitro organoid tissue fragmentation device of the present invention, by respectively arranging a variable frequency motor 201 and a battery 202 inside a pen-shaped protective shell 101 to drive a transmission rod 203 to drive a fragmentation blade 204 to rotate and fragment tissue cells, has a high degree of integration and a small size, and can directly perform fragmentation operations in a test tube, thereby achieving the effect of fragmenting a small amount of tissue.

[0079] A reset elastic member 301 is provided inside the protective shell 101 to apply rebound pressure to the variable frequency motor 201, and a rotating base 302, a fixed limiting cylinder 303 and a pressure cylinder 304 are provided inside the protective shell 101 to cooperate with each other to limit the position of the variable frequency motor 201, so that the crushing cutter head 204 can be extended and retracted inside the protective shell 101, thereby ensuring that the crushing cutter head 204 remains clean and safe when not in use.

[0080] By installing a negative pressure chamber 401 at the end of the protective shell 101, plugging the connector 402 at the end of the negative pressure chamber 401 into the transmission rod 203, and installing a slidable piston member 403 inside the negative pressure chamber 401 for extracting and injecting liquid, it is possible to conveniently add tissue fluid when crushing the sample tissue.

[0081] By setting a damping hole 4031 and a scale mark 4032 on the surface of the piston member 403, and cooperating with the damping member 405 and the damping hole 4031 to provide a stroke prompt, when extracting or injecting tissue fluid, the measurement can be judged according to the scale mark 4032, and the measurement can be determined by the prompt sound and resistance of the damping member 405, thereby achieving the effect of precise control of measurement.

[0082] By setting up a support base 501 and a test tube rack 502, a heating plate 504 is set on the support base 501 to heat the liquid inside the liquid cavity 505, thereby heating and keeping the sample tissue warm. When storing the sample, it can be placed in a low-temperature storage. The liquid in the liquid cavity 505 can keep the sample at a low temperature for a certain period of time, thereby achieving the control of low-temperature storage of the sample tissue and heating to enhance cell activity.

[0083] Therefore, the present invention effectively overcomes various shortcomings in the prior art and has high industrial utilization value.

[0084] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A test tube organoid tissue fragmentation device, characterized in that: include: Protection mechanism, crushing mechanism, height locking mechanism and liquid taking mechanism; The crushing mechanism and the height locking mechanism are both installed inside the protection mechanism, and the height locking mechanism can push the crushing mechanism to move so that the end of the crushing mechanism extends out from the inside of the protection mechanism and is limited; The liquid taking mechanism is arranged at the other end of the protection mechanism, and the end of the liquid taking mechanism is plugged into the end of the crushing mechanism; The crushing mechanism comprises a variable frequency motor (201), a storage battery (202), a transmission rod (203) and a crushing cutter head (204); the variable frequency motor (201) and the storage battery (202) are electrically connected; the transmission rod (203) is connected to the rotor of the variable frequency motor (201); and two ends of the transmission rod (203) are respectively located at the mouths of two ends of the protection mechanism; The height locking mechanism comprises a resetting elastic member (301) and a locking component, the locking component can drive the variable frequency motor (201) and the storage battery (202) to move, so that the crushing cutter head (204) can be extended and retracted, and the axis of the locking component is penetrated by the transmission rod (203); The resetting elastic member (301) is sleeved on the outer surface of the transmission rod (203), and the two ends of the resetting elastic member (301) are respectively overlapped with the end of the variable frequency motor (201) and the inner wall of the protection mechanism.

2. The in vitro organoid tissue fragmentation device according to claim 1, characterized in that: The crushing cutter head (204) is detachably mounted on the end of the transmission rod (203), and the axes of the transmission rod (203) and the crushing cutter head (204) are both hollow; The storage battery (202) is provided with a frequency conversion switch (205) and a charging port (206), respectively. The frequency conversion switch (205) and the charging port (206) both extend to the outer surface of the protection mechanism, and the frequency conversion switch (205) can control the power supply and operating frequency of the frequency conversion motor (201).

3. The in vitro organoid tissue fragmentation device according to claim 2, characterized in that: The protection mechanism comprises a protection shell (101), and a telescopic sliding groove (102) is provided on the outer surface of the protection shell (101); The locking component comprises: a rotating base (302), a fixed limiting cylinder (303) and a pressure cylinder (304); the rotating base (302), the fixed limiting cylinder (303) and the pressure cylinder (304) are sleeved together, and a hand-pushing block (305) is provided on the outer surface of the pressure cylinder (304); the hand-pushing block (305) extends to the outer surface of the protection mechanism; The frequency conversion switch (205), the charging port (206) and the push block (305) all pass through the telescopic slide groove (102) and can slide in the telescopic slide groove (102).

4. The in vitro organoid tissue fragmentation device according to claim 3, characterized in that: A supporting step (103) and a limiting groove (104) are provided inside the protective shell (101); a limiting slider (207) is provided on the outer surface of the variable frequency motor (201); the limiting slider (207) is slidably connected to the limiting groove (104); and one end of the reset elastic member (301) overlaps the supporting step (103).

5. The in vitro organoid tissue fragmentation device according to claim 3, characterized in that: The outer surface of the protective shell (101) is provided with a clamping piece (105), and the clamping piece (105) is elastic.

6. The in vitro organoid tissue fragmentation device according to claim 1, characterized in that: The liquid extraction mechanism comprises a negative pressure chamber (401), a connector (402), a piston member (403) and a rebound member (404); the connector (402) is fixed at one end of the negative pressure chamber (401) and is communicated with the interior of the negative pressure chamber (401); the piston member (403) extends into the interior of the negative pressure chamber (401), and the piston member (403) can slide inside the negative pressure chamber (401) and compress air; the rebound member (404) is located inside the negative pressure chamber (401) and can apply a rebound force to the piston member (403).

7. The in vitro organoid tissue fragmentation device according to claim 6, characterized in that: The connector (402) is provided with a sleeve groove (4021) inside, the end of the transmission rod (203) can be inserted into the sleeve groove (4021), and the upper end of the transmission rod (203) can slide axially along the inside of the sleeve groove (4021).

8. The in vitro organoid tissue fragmentation device according to claim 6, characterized in that: The piston member (403) comprises a piston end and a push handle, wherein the piston end is located inside the negative pressure chamber (401), and the push handle extends to the outer surface of the negative pressure chamber (401); The push handle is provided with a damping hole (4031) and a scale mark (4032), and the damping hole (4031) and the scale mark (4032) are distributed along the extension direction of the push handle; A damping member (405) is disposed inside the negative pressure chamber (401), and the damping member (405) cooperates with the damping hole (4031) to indicate the movement stroke of the piston member (403).

9. The in vitro organoid tissue fragmentation device according to claim 8, characterized in that: The damping member (405) comprises a damping telescopic cylinder (4051), a damping spring (4052) and a damping rod (4053); the damping spring (4052) is installed inside the damping telescopic cylinder (4051) to apply pressure to one end of the damping rod (4053); the other end of the damping rod (4053) passes through the outside of the damping telescopic cylinder (4051); and the damping telescopic cylinder (4051) can be plugged into the damping hole (4031).

10. A sample tissue temperature control accessory for a test tube, characterized in that: The temperature control accessory can be used in conjunction with the crushing device according to any one of claims 1 to 9, and the temperature control accessory comprises a support base (501) and a test tube rack (502), wherein the test tube rack (502) is arranged above the support base (501), and a plurality of test tube holes (503) are arranged on the top of the test tube rack (502) for fixing test tubes; A heating plate (504) is arranged between the support base (501) and the test tube rack (502); a power supply is arranged inside the support base (501) and the power supply can supply power to the heating plate (504); a liquid cavity (505) is arranged inside the test tube rack (502); a heat conducting plate (506) is arranged at the bottom of the liquid cavity (505); the heating plate (504) and the heat conducting plate (506) are in conflict with each other; The interior of the liquid chamber (505) is filled with liquid.

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