Gas phase liquid nitrogen biological container temperature distribution and liquid level calibration method and device

Abstract

The invention discloses a gas phase liquid nitrogen biological container temperature distribution and liquid level calibration method and device, and relates to the field of equipment calibration, the gas phase liquid nitrogen biological container comprises a gas phase liquid nitrogen biological container main body, the top end of the gas phase liquid nitrogen biological container main body is provided with a detection cover, and the top end of the detection cover is provided with a heat preservation pad. The temperature sensors, the sensor host and the positioning pipe are arranged, the temperature sensors with different depths are inserted into the detection cover of the gas-phase liquid nitrogen biological container main body, on-site temperature distribution and liquid level calibration can be carried out, the temperature distribution in the gas-phase storage area of the gas-phase liquid nitrogen biological container main body is obtained through software system analysis, and the accuracy of the gas-phase liquid nitrogen biological container main body is improved. Through combination with the device and a pre-cooling method, temperature distribution and liquid level calibration of the gas-phase liquid nitrogen biological container main body are carried out in situ under the vacuum working condition, and the problems that a large amount of liquid nitrogen is evaporated, the temperature fluctuation is large, and the temperature stability is difficult to recover due to the fact that a heat source with the huge temperature difference is introduced into the gas-phase liquid nitrogen biological container main body are solved.

Description

Technical Field

[0001] The invention relates to the field of equipment calibration, and in particular to a method and a device for calibrating the temperature distribution and liquid level of a gas-phase liquid nitrogen biological container. Background Art

[0002] Gas-phase liquid nitrogen biological containers can store and manage various biological samples such as human in vitro organs, tissues, cells, blood, body fluids, secretions, excretions and their biological macromolecular derivatives. As an important carrier for the preservation of biological resources, its most critical technical indicator is the temperature distribution in the gas phase area. Biological samples have extremely strict temperature requirements. Improper storage temperature may cause changes in the characteristics of biological samples, and even cause the preservation of samples to fail. Even a slight temperature change may affect the activity and stability of the samples. At the same time, the temperature control of the gas-phase liquid nitrogen biological container relies on liquid nitrogen as a cold source. The bottom of the gas-phase liquid nitrogen biological container is liquid nitrogen. Only when the liquid nitrogen is within a certain liquid level range can the gas phase storage area be guaranteed. The temperature distribution meets the preset temperature range. The gas-phase liquid nitrogen biological container itself is generally equipped with temperature control sensors, temperature monitoring sensors, and liquid level monitoring sensors, but these sensors are solidified inside the gas-phase liquid nitrogen biological container, most of which cannot be removed and have no way to trace the source. In addition, there will be drift during use, resulting in inaccurate monitored temperature and liquid level. Removable ones, such as liquid level or temperature sensors in the form of external bottle stoppers, will cause the equipment to lose monitoring during the disassembly and traceability process, and there is a risk of loss of control. There is a risk of introducing errors during disassembly and reinstallation. The traceability, that is, the calibration result, has no practical guiding value. Therefore, it is particularly important to regularly perform temperature and liquid level calibration tests on gas-phase liquid nitrogen biological containers.

[0003] The temperature of the storage area of ​​the gas phase liquid nitrogen biological container is generally as low as (-195~-150)℃. Traditional wireless temperature measurement equipment cannot work and store for a long time under this low temperature condition. At present, the temperature calibration of the automated deep cryogenic sample preservation system can only be carried out by disassembling its temperature sensor for comparison, which cannot meet the temperature calibration of the automated deep cryogenic sample preservation system under real working conditions. There are several problems when using wired sensors such as patrol meters to be deployed through the cover: the deployment position cannot be determined and the cover cannot be tightly sealed. Since the temperature sensor placed in it is generally put in at room temperature, there is a temperature difference of nearly 200℃ with the internal temperature, which is equivalent to introducing a heat source, which will cause the liquid nitrogen to continue to evaporate, the temperature change caused by the introduction of the heat source is drastic, and the temperature field is difficult to restore stability. Therefore, a method and device for temperature distribution and liquid level calibration of a gas phase liquid nitrogen biological container are urgently needed. Summary of the invention

[0004] The purpose of the present invention is to provide a method and device for calibrating the temperature distribution and liquid level of a gas phase liquid nitrogen biological container in order to solve the problems that the liquid level calibration of the existing gas phase liquid nitrogen biological container is generally carried out by using a liquid level ruler, which has the disadvantages of low accuracy, manual operation, large reading errors, and opening the cover to detect liquid nitrogen evaporation.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a gas phase liquid nitrogen biological container temperature distribution and liquid level calibration device, comprising a gas phase liquid nitrogen biological container body, a detection cover is arranged at the top of the gas phase liquid nitrogen biological container body, a heat preservation pad is arranged at the top of the detection cover, a positioning tube is engaged with the inner side of the heat preservation pad, a magnetic fixing sleeve is installed at the bottom end of the positioning tube, and a sensor host is arranged at the top of the positioning tube, and a temperature sensor is installed on the magnetic fixing sleeve.

[0006] Preferably, a magnetic single-hole shell base is provided at the top of the sensor host, and the number of sensor hosts is ten, the magnetic fixing sleeve and the temperature sensor penetrate into the interior of the gas phase liquid nitrogen biological container body, a magnetic shell is provided on the outside of the sensor host, a sensor seat is provided on the temperature sensor, and the temperature sensor and the sensor host are magnetically installed through the sensor seat, the magnetic fixing sleeve and the magnetic shell, and the sensor host is composed of a PCB circuit board, a battery compartment, a PEEK single-hole upper cover, and a magnetic bottom cover.

[0007] A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container, comprising the following steps: Step 1: Start the calibration device: Step 2: primary precooling; Step 3: Secondary gradient precooling; Step 4: Calibration device placement and data collection; Step 5: Data reading and temperature field distribution calibration: Step 6: Liquid level calibration; Step 7: The calibration software automatically generates a gas phase liquid nitrogen biocontainer calibration result report.

[0008] Preferably, in step one, the temperature sensor is connected to the magnetic fixing sleeve, and the software side identifies the temperature sensor temperature recorder endpoint and performs collection settings. The sampling interval and working time can be set, such as setting the sampling interval to 10s and the working time to 1h.

[0009] Preferably, in step 2, 10 temperature sensors 6 are inserted into a low-temperature thermostat for primary precooling, and the low-temperature thermostat is set to -50°C for 1 hour. In step 3, the low-temperature thermostat is set to -80°C for 1 hour; -180°C for 25 minutes. The software acquisition end can stably collect a stable temperature of -180°C for more than 15 minutes, and the stable judgment is -180±1.0°C.

[0010] Preferably, in step 4, the detection cover replaces the original cover / plug of the gas phase liquid nitrogen biological container to be tested, and the recorder endpoint temperature sensor that has completed the secondary gradient pre-cooling is quickly inserted into the detection hole of the detection cover. The temperature sensors are arranged inside the main body of the gas phase liquid nitrogen biological container through the positioning holes. One of the temperature sensors is inserted into the liquid nitrogen just immersed in it. According to the software system, it can be seen in real time that the temperature of the temperature sensor is stable at -196°C liquid nitrogen temperature, which is recorded as A0. The remaining 9 are equally spaced according to the height of the gas phase storage area and arranged vertically; the software end automatically determines whether the temperature of the main storage area of ​​the gas phase liquid nitrogen biological container has reached stability according to the real-time temperature collected by each temperature sensor recorder endpoint. The judgment principle is that after receiving data for 10 minutes, the temperature fluctuation is recorded once every accumulated minute. When the 10-minute temperature fluctuation of all temperature recording endpoints is less than 1.0°C, the software end determines that the gas phase liquid nitrogen biological container has reached thermal equilibrium and the temperature is stable, and starts timing. After continuing to record the temperature for 15 minutes, it prompts that the calibration process is completed.

[0011] Preferably, in step five, after the calibration process is completed, the software reads all recorded temperatures of each temperature sensor recorder endpoint, and automatically cuts the stable temperature field data of the last 15 minutes, and calculates the top maximum temperature, temperature indication error, and vertical uniformity calibration results according to the temperature sensor position marked during deployment.

[0012] Preferably, in step six, the liquid level of the current gas-phase liquid nitrogen biological container body is calculated through the temperature jump of the liquid nitrogen gas-liquid interface built into the software system, and compared with the built-in liquid level monitoring result of the gas-phase liquid nitrogen biological container body to obtain the liquid level indication error.

[0013] Preferably, the liquid level indication error calculation formula is:

[0014] Where: ——Liquid level indication error of gas phase liquid nitrogen biological container (mm); ——The average value of the liquid level displayed by the gas phase liquid nitrogen biological container monitoring (mm); ——Convert the liquid level result of A and A measured temperature (mm).

[0015] Compared with the prior art, the present invention has the following beneficial effects: The present invention arranges a temperature sensor, a sensor host and a positioning tube, inserts temperature sensors of different depths into a detection cover of a gas phase liquid nitrogen biological container body, and can perform on-site temperature distribution and liquid level calibration. Through software system analysis, the temperature distribution in a gas phase storage area of ​​the gas phase liquid nitrogen biological container body is obtained. Through combination with a device and a precooling method, the temperature distribution and liquid level calibration of the gas phase liquid nitrogen biological container body are performed in situ under vacuum conditions, thereby avoiding the problems of large amount of liquid nitrogen evaporation, large temperature fluctuation, difficulty in restoring temperature stability, etc. caused by the introduction of a heat source with a huge temperature difference into the gas phase liquid nitrogen biological container body. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main structure of the gas phase liquid nitrogen biological container of the present invention; Figure 2 It is a schematic diagram of the positioning tube of the present invention; Figure 3 It is a schematic diagram of a thermal insulation pad of the present invention.

[0017] In the figure: 1. Gas phase liquid nitrogen biological container body; 2. Detection cover; 3. Insulation pad; 4. Positioning tube; 5. Magnetic fixing sleeve; 6. Temperature sensor; 7. Sensor host; 8. Magnetic single-hole shell base. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-Figure 3A method and device for calibrating the temperature distribution and liquid level of a gas phase liquid nitrogen biological container, comprising a gas phase liquid nitrogen biological container body 1, a detection cover 2 is arranged at the top of the gas phase liquid nitrogen biological container body 1, a heat preservation pad 3 is arranged at the top of the detection cover 2, a positioning tube 4 is engaged with the inner side of the heat preservation pad 3, a magnetic fixing sleeve 5 is installed at the bottom end of the positioning tube 4, and a sensor host 7 is arranged at the top of the positioning tube 4, a temperature sensor 6 is installed on the magnetic fixing sleeve 5, a magnetic single-hole shell base 8 is arranged at the top of the sensor host 7, and the number of the sensor hosts 7 is ten, the magnetic fixing sleeve 5 and the temperature sensor 6 penetrate into the interior of the gas phase liquid nitrogen biological container body 1, a magnetic shell is arranged on the outer side of the sensor host 7, a sensor seat is arranged on the temperature sensor 6, and the temperature sensor 6 is provided with a magnetic suction housing. The temperature sensor 6 and the sensor host 7 are magnetically installed through the sensor seat, the magnetic fixing sleeve 5 and the magnetic shell. The sensor host 7 is composed of a PCB circuit board, a battery compartment, a PEEK single-hole upper cover, and a magnetic bottom cover. The device includes a hardware part and a software system. The temperature sensors 6 of different depths are inserted into the detection cover 2 of the gas phase liquid nitrogen biological container body 1, and the temperature distribution and liquid level calibration can be performed on site. During calibration, the calibration device replaces the original sealing cover, and the actual working condition of the gas phase liquid nitrogen biological container body 1 can still be guaranteed. The temperature distribution is collected, and the temperature distribution in the gas phase storage area of ​​the gas phase liquid nitrogen biological container body 1 is obtained through software system analysis: including temperature deviation, temperature vertical uniformity, temperature horizontal uniformity, and temperature fluctuation;The liquid level of the current gas-phase liquid nitrogen biological container body 1 is calculated through the temperature jump of the liquid nitrogen gas-liquid junction built into the software system, and compared with the built-in liquid level monitoring result of the gas-phase liquid nitrogen biological container body 1 to obtain the liquid level indication error. The device can be calibrated on-site, has value traceability, can be calibrated to the actual working conditions, can simultaneously obtain temperature and liquid level calibration results, is easy to use, and has 10 detection holes distributed on the detection cover 2, the number of which can be modified / expanded, generally preferably 10 or multiples of 10, 10 temperature sensors 6, one of which is inserted into the liquid nitrogen just immersed, according to the software system, it can be seen in real time that the temperature of the temperature sensor 6 is stable at -196°C (liquid nitrogen temperature), and the remaining 9 are equally spaced according to the height of the gas phase storage area and arranged vertically. The positioning tube 4 can ensure that the inserted temperature sensor 6 can be fixedly suspended in the gas phase area to collect the temperature of the area. At the same time, it ensures sealing to avoid heat leakage. The sensor host 7 of each temperature sensor 6 is placed on the detection cover 2 outside, that is, in the ambient temperature, the sensor host 7 plus the temperature sensor 6 together constitute a temperature recording endpoint, which can transmit the collected temperature in the storage area of ​​the gas phase liquid nitrogen biological container body 1 to the software end in real time, and can also be stored in the sensor host 7, which is convenient for subsequent data reading and avoids packet loss. The connection between the temperature sensor 6 and the sensor host 7 is connected by magnetic attraction. The temperature sensor 6 and the sensor host 7 are magnetically installed through the sensor seat, the magnetic fixing sleeve 5 and the magnetic shell. When contacting the magnetic attraction, the sensor host 7 and the temperature sensor 6 are electrically connected by electromagnetic means. After the magnetic attraction is established, the software end can identify, display and control the temperature recording endpoint. In actual use, the magnetic connection can be made according to the required number. Another advantage of this structural method is that when the temperature sensor 6 or the sensor host 7 has a fault, it does not need to be repaired as a whole, and can be directly matched with another host / sensor for use, thereby improving the turnover rate of the equipment and reducing the maintenance cost. ;

[0020] A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container, comprising the following steps: Step 1: Start the calibration device: Step 2: primary precooling; Step 3: Secondary gradient precooling; Step 4: Calibration device placement and data collection; Step 5: Data reading and temperature field distribution calibration: Step 6: Liquid level calibration; Step 7: The calibration software automatically generates a gas phase liquid nitrogen biocontainer calibration result report; In step 1, the temperature sensor 6 is connected to the magnetic fixing sleeve 5, and the software end identifies the temperature recorder endpoint of the temperature sensor 6, performs collection settings, and can set the sampling interval and working time, such as setting the sampling interval to 10s and the working time to 6h; In step 2, 10 temperature sensors 6 are inserted into the cryostat for primary precooling. The cryostat is set to -50°C for 1 hour. In step 3, the cryostat is set to -80°C for 1 hour and -180°C for 25 minutes. The software acquisition end can stably acquire a stable temperature of -180°C for more than 15 minutes, and the stable judgment is -180±1.0°C. In step 4, the detection cover 2 replaces the original cover / plug of the gas phase liquid nitrogen biological container to be tested, and the recorder endpoint temperature sensor 6 that has completed the secondary gradient precooling is quickly inserted into the detection hole of the detection cover 2. The temperature sensor 6 is arranged inside the gas phase liquid nitrogen biological container body 1 through the positioning hole. One of the temperature sensors 6 is inserted into the liquid nitrogen just immersed. According to the software system, it can be seen in real time that the temperature of the temperature sensor 6 is stable at -196°C liquid nitrogen temperature, recorded as A0, and the remaining 9 are equally spaced according to the height of the gas phase storage area and arranged vertically; the software end automatically determines whether the temperature of the storage area of ​​the gas phase liquid nitrogen biological container body 1 has reached stability based on the real-time temperature collected by each temperature sensor 6 recorder endpoint. The judgment principle is that after receiving data for 10 minutes, the temperature fluctuation is recorded every 10 minutes. When the 10-minute temperature fluctuation of all temperature recording endpoints is less than 1.0°C, the software end determines that the gas phase liquid nitrogen biological container has reached thermal equilibrium and the temperature is stable, and starts timing. After continuing to record the temperature for 15 minutes, it prompts that the calibration process is completed. Among them, the height of the gas phase storage area of ​​the liquid nitrogen biological container to be tested is 120cm, and the freezing box placement area is 100cm from the bottom to the top. The top temperature position sensor is placed 30cm away from the top tank mouth, that is, the highest position of the freezing box, recorded as A1; the other 8 are arranged at equal distances, and the vertical distances are located at 40cm, 50cm, 60cm... from the tank mouth, recorded as A2, A3, A4...; In step 5, after the calibration process is completed, the software reads all recorded temperatures of the end points of each temperature sensor 6 recorder, and automatically cuts the stable temperature field data of the last 15 minutes. According to the position of the temperature sensor 6 marked during deployment, the top maximum temperature, temperature indication error, and vertical uniformity calibration results are calculated respectively. The maximum temperature at the top is: after the main body 1 of the gas phase liquid nitrogen biological container reaches thermal equilibrium, the maximum temperature measured by the endpoint A1 of all top recorders within 15 minutes is the maximum temperature at the top of the main body 1 of the gas phase liquid nitrogen biological container.

[0021] Where: —Maximum temperature of the top of the gas phase liquid nitrogen biocontainer (°C); ——The temperature value of the A0 temperature measuring point of the gas phase liquid nitrogen biological container at a certain moment (℃).

[0022] Temperature indication error: After the gas phase liquid nitrogen biological container body 1 reaches thermal equilibrium, the difference between the arithmetic mean of the monitored displayed temperature within 10 minutes and the arithmetic mean of the temperature of the center position recorder endpoint (A5 in this embodiment) is the temperature indication error.

[0023] Where: ——Temperature indication error of gas phase liquid nitrogen biological container (℃); ——The average value of the temperature displayed by the gas phase liquid nitrogen biological container monitoring (℃); ——The average temperature of the central location recorder endpoint (A5 in this embodiment) (℃); Vertical uniformity: Except for the temperature sensor A0 placed in liquid nitrogen, the maximum value of the difference between the other recorder end position sensors.

[0024] Where: ——Vertical temperature uniformity of gas phase liquid nitrogen biological container (℃); —The absolute value of the difference between the arithmetic mean values ​​of the temperatures of A1 and A2 in the gas phase liquid nitrogen biological container within 15 minutes (°C); In step 6, the liquid level of the current gas-phase liquid nitrogen biological container body 1 is calculated through the temperature jump of the liquid nitrogen gas-liquid interface built into the software system, and compared with the built-in liquid level monitoring result of the gas-phase liquid nitrogen biological container body 1 to obtain the liquid level indication error; The calculation formula of liquid level indication error is:

[0025] Where: ——Liquid level indication error of gas phase liquid nitrogen biological container (mm); ——The average value of the liquid level displayed by the gas phase liquid nitrogen biological container monitoring (mm); ——The result of converting the measured temperature of A9 and A0 into liquid level (mm); Through the combination with the device and the pre-cooling method, the temperature distribution and liquid level calibration of the gas-phase liquid nitrogen biological container can be realized under vacuum conditions and on-site, avoiding the introduction of heat sources with huge temperature differences into the liquid nitrogen biological container, which may cause large evaporation of liquid nitrogen, large temperature fluctuations, and difficulty in restoring temperature stability.

[0026] Working principle: When the present invention is used, the device includes a hardware part and a software system. The temperature sensors 6 of different depths are inserted into the detection cover 2 of the gas phase liquid nitrogen biological container body 1, and the temperature distribution and liquid level calibration can be performed on site. During calibration, the calibration device replaces the original sealing cover, and the actual working condition of the gas phase liquid nitrogen biological container body 1 can still be guaranteed. The temperature distribution is collected, and the temperature distribution in the gas phase storage area of ​​the gas phase liquid nitrogen biological container body 1 is obtained through software system analysis: including temperature deviation, temperature vertical uniformity, temperature horizontal uniformity, and temperature fluctuation; the temperature of the liquid nitrogen gas-liquid junction position built into the software system is The device can be calibrated on-site, has value traceability, can be calibrated for real working conditions, can simultaneously obtain temperature and liquid level calibration results, and is easy to use. Through the combination with the device and the pre-cooling method, the temperature distribution and liquid level calibration of the gas-phase liquid nitrogen biological container body 1 can be realized in vacuum conditions and on-site, so as to avoid the introduction of a heat source with a huge temperature difference into the gas-phase liquid nitrogen biological container body 1, which causes a large amount of liquid nitrogen to evaporate, a large temperature fluctuation, and difficulty in restoring temperature stability.

[0027] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A gas phase liquid nitrogen biological container temperature distribution and liquid level calibration device, comprising a gas phase liquid nitrogen biological container body (1), characterized in that: A detection cover (2) is arranged at the top of the gas phase liquid nitrogen biological container body (1), a heat preservation pad (3) is arranged at the top of the detection cover (2), a positioning tube (4) is engaged with the inner side of the heat preservation pad (3), a magnetic attraction fixing sleeve (5) is installed at the bottom end of the positioning tube (4), and a sensor host (7) is arranged at the top of the positioning tube (4), and a temperature sensor (6) is installed on the magnetic attraction fixing sleeve (5).

2. The device for calibrating temperature distribution and liquid level of a gas-phase liquid nitrogen biological container according to claim 1, characterized in that: A magnetic single-hole housing base (8) is provided at the top of the sensor main unit (7), and the number of the sensor main units (7) is ten. The magnetic fixing sleeve (5) and the temperature sensor (6) penetrate into the interior of the gas phase liquid nitrogen biological container body (1). A magnetic housing is provided on the outside of the sensor main unit (7). A sensor seat is provided on the temperature sensor (6), and the temperature sensor (6) and the sensor main unit (7) are magnetically mounted via the sensor seat, the magnetic fixing sleeve (5) and the magnetic housing. The sensor main unit (7) is composed of a PCB circuit board, a battery compartment, a PEEK single-hole upper cover, and a magnetic bottom cover.

3. A method for calibrating the temperature distribution and liquid level of a gas-phase liquid nitrogen biological container, applied to a device for calibrating the temperature distribution and liquid level of a gas-phase liquid nitrogen biological container as claimed in any one of claims 1 to 2, comprising the following steps: Step 1: Start the calibration device: Step 2: primary precooling; Step 3: Secondary gradient precooling; Step 4: Calibration device placement and data collection; Step 5: Data reading and temperature field distribution calibration: Step 6: Liquid level calibration; Step 7: The calibration software automatically generates a gas phase liquid nitrogen biocontainer calibration result report.

4. A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container according to claim 3, characterized in that: In the step 1, the temperature sensor (6) is connected to the magnetic fixing sleeve (5), and the software end identifies the temperature recorder endpoint of the temperature sensor (6) and performs data collection settings. The sampling interval and working time can be set, such as setting the sampling interval to 10 seconds and the working time to 6 hours.

5. A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container according to claim 3, characterized in that: In the step 2, 10 temperature sensors (6) are inserted into a low temperature thermostat for primary precooling. The low temperature thermostat is set to -50°C for 1 hour. In the step 3, the low temperature thermostat is set to -80°C for 1 hour and -180°C for 25 minutes. The software acquisition end can stably acquire a stable temperature of -180°C for more than 15 minutes, and the stability is judged to be (-180±1.0)°C.

6. A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container according to claim 3, characterized in that: In the fourth step, the detection cover (2) replaces the original cover / plug of the gas phase liquid nitrogen biological container to be tested, and the recorder endpoint temperature sensor (6) that has completed the secondary gradient precooling is quickly inserted into the detection hole of the detection cover (2). The temperature sensor (6) is arranged inside the gas phase liquid nitrogen biological container body (1) through the positioning hole. One of the temperature sensors (6) is inserted into the liquid nitrogen just immersed. According to the software system, it can be seen in real time that the temperature of the temperature sensor (6) is stable at -196°C (liquid nitrogen temperature), which is recorded as A0. The remaining 9 are equally spaced according to the height of the gas phase storage area and arranged vertically. The software end automatically determines whether the temperature of the storage area of ​​the gas phase liquid nitrogen biological container body (1) has reached stability based on the real-time temperature collected by the recorder endpoint of each temperature sensor (6). The judgment principle is that after receiving data for 10 minutes, the temperature fluctuation is recorded once every 10 minutes. When the 10-minute temperature fluctuation of all temperature recording endpoints is less than 1.0°C, the software end determines that the gas phase liquid nitrogen biological container has reached thermal equilibrium and the temperature is stable, and starts timing. After continuing to record the temperature for 15 minutes, it prompts that the calibration process is completed.

7. A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container according to claim 3, characterized in that: In step 5, after the calibration process is completed, the software reads all recorded temperatures of the recorder endpoints of each temperature sensor (6), and automatically cuts the stable temperature field data of the last 15 minutes, and calculates the top maximum temperature, temperature indication error, and vertical uniformity calibration results according to the position of the temperature sensor (6) marked during deployment.

8. The method for calibrating temperature distribution and liquid level of a gas-phase liquid nitrogen biological container according to claim 3, characterized in that: In step six, the liquid level of the current gas phase liquid nitrogen biological container body (1) is calculated based on the temperature jump of the liquid nitrogen gas-liquid interface built into the software system, and compared with the built-in liquid level monitoring result of the gas phase liquid nitrogen biological container body (1) to obtain the liquid level indication error.

9. A method for calibrating temperature distribution and liquid level of a gas phase liquid nitrogen biological container according to claim 8, characterized in that: The calculation formula of the liquid level indication error is: ； Where: ——Liquid level indication error of gas phase liquid nitrogen biological container (mm); ——The average value of the liquid level displayed by the gas phase liquid nitrogen biological container monitoring (mm); ——Convert the liquid level result of the measured temperature of A9 and A0 (mm).

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