A device and method for rinsing a post-frozen section tissue section antibody

Abstract

The application provides a kind of rinsing device and rinsing method of post-freezing section tissue section antibody, belong to biomedical field. Including rinsing fluid tank and screen support plate, the screen support plate is opened with multiple placing holes, multiple the organization screen is arranged in the placing hole, the first drive assembly for driving rinsing fluid is arranged on the rinsing fluid tank. The height of screen support plate is adjusted by lifting assembly, multiple organization screens are lowered into rinsing fluid tank, multiple rotating shafts and fan blades are located in the gap, then, start the first motor to drive one of the rotating shafts to rotate, through the action of pulley and transmission belt, drive multiple rotating shafts to rotate simultaneously, multiple rotating shafts drive circular block and fan blade to rotate, make rinsing fluid in rinsing fluid tank flow from one end to the other end through the gap, in turn circulation, make rinsing fluid enhance the rinsing effect of tissue section in organization screen.

Description

TECHNICAL FIELD

[0001] The present application relates to the biomedical field, more particularly to a kind of ice slice post-tissue slice antibody's rinsing device and rinsing method. BACKGROUND

[0002] Ice slice is a kind of commonly used tissue slice technology, it uses low temperature to freeze and harden tissue quickly, then it is cut into thin slice using slicing machine, this kind of technology can preserve the morphology and structure of tissue, while reducing the loss of antigen, so it is widely used in immunohistochemistry and immunofluorescence etc.Experiments, its purpose is to remove unbound antibody and impurities in washing liquid, to reduce background staining and non-specific coloring, improve the accuracy and reliability of experiment;

[0003] The existing ice slice post-tissue slice antibody's rinsing device, in the process of using, usually pour the rinsing liquid tank into appropriate rinsing liquid, then put tissue screen into screen receiving plate, adjust the assembled screen receiving plate to appropriate height by lifting mechanism, make tissue slice float in rinsing liquid, keep rinsing liquid in flowing state by circulating mechanism, rinse tissue slice, but in the process of rinsing, it is easy to cause tissue slice to be directly impacted by rinsing liquid, so as to cause tissue slice to be easily damaged in rinsing. SUMMARY

[0004] In view of the problems in the prior art that in the rinsing process, tissue slice is directly impacted by rinsing liquid, so as to cause tissue slice to be easily damaged in rinsing, the purpose of the present application is to provide a kind of ice slice post-tissue slice antibody's rinsing device and rinsing method.

[0005] To solve the above problems, the present application adopts the following technical solutions:

[0006] A kind of ice slice post-tissue slice antibody's rinsing device and rinsing method, including rinsing liquid tank and screen receiving plate, characterized by, multiple placing holes are set in the screen receiving plate, multiple tissue screens are arranged in the placing holes, first driving assembly for driving rinsing liquid is arranged on the rinsing liquid tank, vibration assembly for vibrating tissue screen is arranged on the screen receiving plate, lifting assembly for lifting screen receiving plate is arranged on the rinsing liquid tank, mounting assembly for mounting screen receiving plate is arranged on the lifting assembly, receiving assembly for receiving rinsing liquid is arranged on the rinsing liquid tank.

[0007] Optionally, the first drive assembly comprises a plurality of rotating shafts, each of which is rotatably connected to one end of the rinsing liquid tank, one side of the rotating shaft is fixedly connected with a circular block, the outer surface of the circular block is fixedly connected with a plurality of fan leaves, a plurality of gaps are arranged between the tissue screens and the plurality of fan leaves, one side of the rinsing liquid tank is fixedly connected with a fixed plate, one side of each of the plurality of rotating shafts away from the rinsing liquid tank is rotatably connected to the fixed plate, one side of the rinsing liquid tank is fixedly connected with a first L-shaped plate, the inner side of the first L-shaped plate is fixedly connected with a first motor, one of the rotating shafts is fixedly connected to the output end of the first motor, the output end of the first motor is rotatably connected to the fixed plate, the outer surface of each of the plurality of rotating shafts is fixedly connected with a belt pulley, and the same transmission belt is arranged between the plurality of belt pulleys.

[0008] Optionally, the bottom end of each of the plurality of tissue screens is fixedly connected with a semicircular block, corresponding two semicircular blocks are fixedly connected with a connecting shaft, the outer surface of the connecting shaft is rotatably connected with a rectangular plate, and the bottom end of the tissue screen is fixedly connected with a blocking plate, the blocking plate is located between the two rectangular plates.

[0009] Optionally, the vibration assembly comprises a connecting frame, the connecting frame is arranged on the screen receiving plate, the front end of the connecting frame is fixedly connected with a second L-shaped plate, the rear end of the second L-shaped plate is rotatably connected with a rotating shaft between the connecting frame, the outer surface of the rotating shaft is fixedly connected with a half gear, the front end of the connecting frame is slidably connected with a rack plate, the rack plate and the rotating shaft are in meshing relationship, the rack plate is fixedly connected with a knocking rod in left-right symmetry, the front end of the connecting frame is fixedly connected with a fixed block in left-right symmetry, the outer surface of the rotating shaft is fixedly connected with a first circular gear, and the output end of the first motor is fixedly connected with a second circular gear.

[0010] Optionally, the lifting assembly comprises two screw rods, each of the two screw rods is rotatably connected to the top end of the rinsing liquid tank, the outer surface of each of the two screw rods is fixedly connected with a worm gear, two connecting plates are arranged on the rinsing liquid tank, one side of one of the connecting plates is fixedly connected with a third L-shaped plate, the inner side of the third L-shaped plate is fixedly connected with a second motor, the output end of the second motor is fixedly connected with a worm, the worm is rotatably connected to one of the connecting plates, the worm and the two worm gears are in cooperation, the top end of the rinsing liquid tank is fixedly connected with two limiting rods, the outer surfaces of the screw rods and the limiting rods are provided with guide blocks, and two of the guide blocks are threadedly connected to the screw rods, and the other two screw rods are slidably connected to the limiting rods.

[0011] Optionally, the mounting assembly comprises four rectangular blocks, the four rectangular blocks are fixedly connected to the four guide blocks respectively, the connecting frame is fixedly connected to the front two rectangular blocks, a connecting column is rotatably connected to the top of each of the four rectangular blocks, a rotating block is fixedly connected to the top of the connecting column, a first magnet is fixedly connected to one side of the rotating block, a second magnet is fixedly connected to the side of the guide block close to the rectangular block, a fourth L-shaped plate is fixedly connected to each of the four corners of the screen receiving plate, and a rectangular groove that matches the rotating block is formed in the fourth L-shaped plate.

[0012] Optionally, the receiving assembly comprises two inclined plates, a surrounding edge is fixedly connected to the top of each of the two inclined plates, a drainage opening is formed in the surrounding edge, and a second driving assembly is arranged on the rinsing liquid tank.

[0013] Optionally, the second driving assembly comprises a bottom plate, the bottom plate is fixedly connected to the bottom end of the rinsing liquid tank, the connecting plate is fixedly connected to the top of the bottom plate, four limiting plates are fixedly connected to the top of the bottom plate, a fifth L-shaped plate is fixedly connected to one side of one of the limiting plates, a third motor is fixedly connected to the inner side of the fifth L-shaped plate, a bidirectional threaded rod is fixedly connected to the output end of the third motor, the bidirectional threaded rod is rotatably penetrated through the one limiting plate, two moving plates are threadedly connected to the bidirectional threaded rod, the two inclined plates are fixedly connected to the moving plates respectively, a guide rod is fixedly connected between the two limiting plates, and the two moving plates are slidably connected to the guide rod.

[0014] Optionally, an inclined surface is arranged on the inner bottom wall of the rinsing liquid tank, a drainage hole is formed in one side of the rinsing liquid tank, a rubber plug is clamped in the drainage hole, and a pull ring is fixedly connected to one side of the rubber plug.

[0015] Optionally, S1: an appropriate amount of rinsing liquid is added into the rinsing liquid tank, and it is ensured that enough rinsing liquid can cover all the tissue sections;

[0016] S2: the tissue sections are placed into the tissue screen to be carried, and the tissue screen is placed into the screen receiving plate;

[0017] S3: the height of the screen receiving plate is adjusted through the lifting assembly, so that the immersion depth of the tissue sections in the rinsing liquid is controlled;

[0018] S4: the rinsing liquid is kept in a flowing state through the circulating assembly, so that the contact area of the rinsing liquid and the tissue sections is increased.

[0019] Compared with the prior art, the technical scheme provided by the application has at least the following beneficial effects:

[0020] In the above scheme, when the rinsing liquid in the rinsing liquid tank needs to be driven, the height of the screen receiving plate and the plurality of tissue screens is adjusted by the lifting assembly, the plurality of tissue screens are lowered into the rinsing liquid tank, the plurality of rotating shafts and the fan blades are located in the gap, then the first motor is started, the first motor drives one of the rotating shafts to rotate, through the action of the belt pulley and the transmission belt, the plurality of rotating shafts can be driven to rotate at the same time, the plurality of rotating shafts drive the circular blocks and the fan blades to rotate at the same time, in the process of rotation of the fan blades, the rinsing liquid in the rinsing liquid tank can flow from one end to the other end through the gap, and circulate in turn, so that the rinsing effect of the rinsing liquid on the tissue slices in the tissue screens is enhanced, and the fan blades can also avoid direct impact of the rinsing liquid on the tissue samples;

[0021] When the tissue screens need to be rotated, the two rectangular plates are vertically downward under the action of gravity, when the first driving assembly is started to drive the rinsing liquid in the rinsing liquid tank, the rinsing liquid can drive the rectangular plates to rotate under the action of the blocking plate, the rectangular plates can drive the tissue screens to rotate in the placing holes, the tissue screens drive the tissue slices to rotate, so that the tissue sections are cleaned more cleanly, and when the tissue screens need to be placed on the platform, the two rectangular plates are rotated in opposite directions respectively, so that the two rectangular plates are in a parallel state, and the tissue screens can be placed on the platform, which is convenient for placing and taking out the tissue slices;

[0022] When the tissue screens need to be vibrated, the lifting assembly drives the screen receiving plate and the tissue screens to move downward, so that the first circular gear and the second circular gear are engaged with each other, then the first motor is started, the output end of the first motor drives the second circular gear to rotate, the second circular gear drives the first circular gear and the rotating shaft to rotate, the rotating shaft drives the half gear to rotate, the half gear drives the rack plate to reciprocate left and right, the rack plate drives the knocking rod to knock the fixed block, so that the fixed block, the connecting frame, the screen receiving plate and the tissue screens vibrate, and the tissue screens can enhance the rinsing effect on the tissue slices in the process of vibration;

[0023] When the height of the screen receiving plate needs to be adjusted, the second motor is started, the output end of the second motor drives the worm to rotate, the worm drives the two worm gears and the screw rod to rotate, the screw rod drives the guide block to move, and the guide block drives the screen receiving plate to move along the length direction of the limiting rod, so that the height of the screen receiving plate is adjusted. BRIEF DESCRIPTION OF DRAWINGS

[0024] The accompanying drawings, which are incorporated herein and constitute part of the specification, illustrate embodiments of the application and, together with the description, further serve to explain the principles of the application and to enable a person skilled in the relevant art to implement and use the application.

[0025] Figure 1 is a schematic diagram of the three-dimensional structure of the present application;

[0026] Figure 2 Structure diagram of the vibration assembly and the lifting assembly of the present application;

[0027] Figure 3 Structure diagram of the placing hole and the tissue screen of the present application;

[0028] Figure 4 Structure diagram of the right section of the rinsing liquid tank of the present application;

[0029] Figure 5 Structure diagram of the first driving assembly of the present application;

[0030] Figure 6 Structure diagram of the gap of the present application;

[0031] Figure 7 Structure diagram of the rectangular plate and the blocking plate of the present application;

[0032] Figure 8 Structure diagram of the first circular gear of the present application;

[0033] Figure 9 Structure diagram of the vibration assembly of the present application;

[0034] Figure 10 Structure diagram of the lifting assembly of the present application;

[0035] Figure 11 Structure diagram of the Figure 10 Structure diagram of the enlarged A;

[0036] Figure 12 Structure diagram of the receiving assembly and the second driving assembly of the present application;

[0037] Figure 13 Structure diagram of the Figure 4 Structure diagram of the enlarged B.

[0038] [Reference signs]

[0039] 101, rinsing liquid tank; 102, screen receiving plate; 103, placing hole; 104, tissue screen; 201, rotating shaft; 202, circular block; 203, fan blade; 204, gap; 205, fixed plate; 206, first L-shaped plate; 207, first motor; 208, pulley; 209, transmission belt; 210, semicircular block; 211, connecting shaft; 212, rectangular plate; 213, blocking plate; 214, connecting frame; 215, second L-shaped plate; 216, rotating shaft; 217, half gear; 218, rack plate; 219, knocking rod; 220, fixed block; 221, first circular gear; 222, second circular gear; 223, screw rod; 224, worm gear; 225, connecting plate; 226, third L-shaped plate; 227, second motor; 228, worm; 229, limiting rod; 230, guide block; 231, rectangular block; 232, connecting column; 233, rotating block; 234, first magnet; 235, second magnet; 236, fourth L-shaped plate; 237, rectangular groove; 238, inclined plate; 239, surrounding edge; 240, drainage opening; 241, bottom plate; 242, limiting plate; 243, fifth L-shaped plate; 244, third motor; 245, bidirectional threaded rod; 246, moving plate; 247, guide rod; 248, inclined surface; 249, drainage hole; 250, rubber plug; 251, pull ring.

[0040] As shown in the drawings, in order to clearly show the structure of the embodiments of the present application, specific structures and devices are marked in the drawings, but this is only for the need of illustration, and is not intended to limit the present application to the specific structures, devices and environments, and those skilled in the art can adjust or modify these devices and environments according to specific needs. DETAILED DESCRIPTION

[0041] The rinsing device and rinsing method for post-frozen section tissue section antibody provided by the present application are described in detail below in combination with the drawings and specific embodiments. It should be noted that, in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and other alternative ways can also be used by those skilled in the art to implement some known technologies; and the drawings are only used to more specifically describe the embodiments, and are not intended to specifically limit the present application.

[0042] It should be noted that, in the specification, "one embodiment", "embodiment", "exemplary embodiment", "some embodiments" and the like indicate that the described embodiments can include specific features, structures or characteristics, but not necessarily every embodiment includes the specific features, structures or characteristics. In addition, when a specific feature, structure or characteristic is described in combination with an embodiment, it should be within the knowledge of those skilled in the related art to achieve such a feature, structure or characteristic in combination with other embodiments (whether or not explicitly described).

[0043] Generally, the terminology can be understood at least in part from usage in context. For example, the term "one or more" as used herein, depending at least in part upon context, can be used to describe any feature, structure, or characteristic in a singular sense or can be used to describe combinations of features, structures or characteristics in a plural sense. Additionally, the term "based on" can be understood as not necessarily being confined to factors that are strictly deterministic but alternatively based at least in part on probabilistic, statistical, or probabilistic and statistical considerations.

[0044] It will be understood that the terms "on", "over", and "above", in the present invention, should be interpreted in the broadest reasonable manner, such that "on" not only means "directly on" something, but also includes the meaning of being "on" something with intervening features or layers therebetween, and "over" or "above" not only means the meaning of being "over" or "above" something, but also can include the meaning of being "over" or "above" something with no intervening features or layers therebetween.

[0045] In addition, spatially relative terms, such as "under", "below", "lower", "over", "upper" and the like, can be used herein for ease of description to describe one element or feature's relationship to another element(s) or feature(s) as illustrated in the figures. The spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientations depicted in the figures. The devices can be otherwise oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein can similarly be interpreted accordingly.

[0046] As shown in Figures 1 to 13 The present application provides a kind of ice frozen section post-tissue section antibody's rinsing device and rinsing method, including rinsing liquid tank 101 and screen support plate 102, multiple placing holes 103 are set in the screen support plate 102, multiple the organization screen 104 is arranged in the placing hole 103, the first drive assembly for driving rinsing liquid is arranged on the rinsing liquid tank 101, the vibration assembly for vibrating organization screen 104 is arranged on the screen support plate 102, the lifting assembly for lifting screen support plate 102 is arranged on the rinsing liquid tank 101, the installation assembly for installing screen support plate 102 is arranged on the lifting assembly, the receiving assembly for receiving rinsing liquid is arranged on the rinsing liquid tank 101.

[0047] When the device needs to be used, an appropriate amount of rinsing liquid is poured into the rinsing liquid tank 101, then the tissue screen 104 is placed into the placing hole 103 on the screen receiving plate 102, the assembled screen receiving plate 102 is placed on the lifting assembly, the screen receiving plate 102 is fixed through the mounting assembly, then the lifting assembly is started, the lifting assembly drives the assembled screen receiving plate 102 to move downward, so that the tissue screen 104 is lowered into the rinsing liquid tank 101, and the tissue slices in the tissue screen 104 are naturally floated in the rinsing liquid. The first driving assembly and the vibration assembly are started, the rinsing liquid in the rinsing liquid tank 101 is driven through the first driving assembly, so that the rinsing effect of the rinsing liquid on the tissue slices in the tissue screen 104 is enhanced. The driving assembly can also avoid direct impact of the rinsing liquid on the tissue sample. The vibration assembly can enhance the rinsing effect of the tissue slices in the tissue screen 104. When the rinsing liquid in the rinsing liquid tank 101 needs to be replaced, the rinsing liquid dropped by the tissue screen 104 can be received through the receiving assembly, so that the rinsing liquid in the main body can be replaced conveniently, a long waiting time is avoided, and all tissues are sorted on the same screen receiving plate 102, so that the level counting and differentiation of the continuous section tissues are facilitated.

[0048] As shown in Figure 4 and Figure 5 , the first driving assembly comprises a plurality of rotating shafts 201, the rotating shafts 201 are rotatably penetrated into one end of the rinsing liquid tank 101, one side of the rotating shaft 201 is fixedly connected with a circular block 202, the outer surface of the circular block 202 is fixedly connected with a plurality of fan leaves 203, a plurality of gaps 204 matched with the plurality of fan leaves 203 are arranged between the plurality of tissue screens 104, one side of the rinsing liquid tank 101 is fixedly connected with a fixed plate 205, one side of the rotating shaft 201 away from the rinsing liquid tank 101 is rotatably connected to the fixed plate 205, one side of the rinsing liquid tank 101 is fixedly connected with a first L-shaped plate 206, the inner side of the first L-shaped plate 206 is fixedly connected with a first motor 207, one of the rotating shafts 201 is fixedly connected to the output end of the first motor 207, the output end of the first motor 207 is rotatably penetrated into the fixed plate 205, the outer surface of the rotating shaft 201 is fixedly connected with a belt pulley 208, and the same transmission belt 209 is arranged between the plurality of belt pulleys 208.

[0049] When it is needed to drive the rinsing liquid in the rinsing liquid tank 101, the height of the screen receiving plate 102 and the plurality of tissue screens 104 is adjusted by the lifting assembly, the plurality of tissue screens 104 is lowered into the rinsing liquid tank 101, the plurality of rotating shafts 201 and the fan blades 203 are located in the gaps 204, then the first motor 207 is started, the first motor 207 drives one of the rotating shafts 201 to rotate, through the action of the belt pulley 208 and the transmission belt 209, the plurality of rotating shafts 201 can be driven to rotate at the same time, the plurality of rotating shafts 201 drives the circular blocks 202 and the fan blades 203 to rotate at the same time, in the rotating process of the fan blades 203, the rinsing liquid in the rinsing liquid tank 101 can be driven from one end to the other end, and is circulated in turn, so that the rinsing effect of the rinsing liquid on the tissue slices in the tissue screens 104 is enhanced, and the driving assembly can also avoid that the rinsing liquid directly impacts the tissue sample.

[0050] As shown in Figure 7 The bottom end of each of the plurality of tissue screens 104 is fixedly connected with a semicircular block 210 which is symmetrically arranged at the front and back, a connecting shaft 211 is fixedly connected between the two semicircular blocks 210 corresponding to each other, a rectangular plate 212 is rotatably connected to the outer surface of the connecting shaft 211, and a blocking plate 213 is fixedly connected to the bottom end of the tissue screen 104 and located between the two rectangular plates 212.

[0051] When it is needed to rotate the tissue screen 104, the two rectangular plates 212 are vertically downward under the action of gravity, when the first driving assembly is started to drive the rinsing liquid in the rinsing liquid tank 101, the rinsing liquid can drive the rectangular plate 212 to rotate under the action of the blocking plate 213, the rectangular plate 212 can drive the tissue screen 104 to rotate in the placement hole 103, the tissue screen 104 drives the tissue slice to rotate, so that the tissue section is cleaned more cleanly, and when it is needed to place the tissue screen 104 on the platform, the two rectangular plates 212 are rotated in opposite directions respectively, so that the two rectangular plates 212 are in a parallel state, and the tissue screen 104 can be placed on the platform, which is convenient for placing and taking out the tissue slice.

[0052] As shown in Figure 8 and Figure 9As shown, the vibration assembly comprises a connecting frame 214 arranged on the screen receiving plate 102, the front end of the connecting frame 214 is fixedly connected with a second L-shaped plate 215, the rear end of the second L-shaped plate 215 is rotatably connected with a rotating shaft 216 between the connecting frame 214, the outer surface of the rotating shaft 216 is fixedly connected with a half gear 217, the front end of the connecting frame 214 is slidably connected with a rack plate 218, the rack plate 218 is meshed with the rotating shaft 216, the left and right symmetrical of the rack plate 218 is fixedly connected with a knocking rod 219, the front end of the connecting frame 214 is fixedly connected with a fixed block 220, the outer surface of the rotating shaft 216 is fixedly connected with a first circular gear 221, the output end of the first motor 207 is fixedly connected with a second circular gear 222.

[0053] When the tissue screen 104 needs to be vibrated, the screen receiving plate 102 and the tissue screen 104 are driven to move downward by the lifting assembly, so that the first circular gear 221 is meshed with the second circular gear 222, then the first motor 207 is started, the output end of the first motor 207 drives the second circular gear 222 to rotate, the second circular gear 222 drives the first circular gear 221 and the rotating shaft 216 to rotate, the rotating shaft 216 drives the half gear 217 to rotate, the half gear 217 drives the rack plate 218 to reciprocate left and right, the rack plate 218 drives the knocking rod 219 to knock the fixed block 220, so that the fixed block 220, the connecting frame 214, the screen receiving plate 102 and the tissue screen 104 vibrate, and the contact area and the rinsing effect of the rinsing liquid and the tissue section can be increased during the vibration of the tissue screen 104.

[0054] As shown in the figure, Figure 10 As shown, the lifting assembly comprises two screw rods 223, both of which are rotatably connected to the top end of the rinsing liquid tank 101, the outer surfaces of both of the screw rods 223 are fixedly connected with worm gears 224, the rinsing liquid tank 101 is provided with two connecting plates 225, one side of one of the connecting plates 225 is fixedly connected with a third L-shaped plate 226, the inner side of the third L-shaped plate 226 is fixedly connected with a second motor 227, the output end of the second motor 227 is fixedly connected with a worm 228, the worm 228 is rotatably penetrated through the one connecting plate 225, the worm 228 cooperates with the two worm gears 224, the top end of the rinsing liquid tank 101 is fixedly connected with two limiting rods 229, the outer surfaces of the screw rods 223 and the limiting rods 229 are provided with guide blocks 230, two of the guide blocks 230 are threadedly connected to the screw rods 223, and the other two of the guide blocks 230 are slidably connected to the limiting rods 229.

[0055] When it is necessary to adjust the height of the screen receiving plate 102, the second motor 227 is started, the output end of the second motor 227 drives the worm 228 to rotate, the worm 228 drives the two worm gears 224 and the screw rod 223 to rotate, the screw rod 223 drives the guide block 230 to move, and the guide block 230 drives the screen receiving plate 102 to move along the length direction of the limiting rod 229, so that the height of the screen receiving plate 102 is adjusted.

[0056] As shown in Figure 11 The installation assembly comprises four rectangular blocks 231, the four rectangular blocks 231 are fixedly connected to the four guide blocks 230 respectively, the connecting frame 214 is fixedly connected to the front two rectangular blocks 231, the top end of each of the four rectangular blocks 231 is rotatably connected with a connecting column 232, the top end of the connecting column 232 is fixedly connected with a rotating block 233, one side of the rotating block 233 is fixedly connected with a first magnet 234, one side of the guide block 230 close to the rectangular block 231 is fixedly connected with a second magnet 235, the four corners of the screen receiving plate 102 are fixedly connected with fourth L-shaped plates 236 respectively, and the fourth L-shaped plates 236 are provided with rectangular grooves 237 matched with the rotating block 233.

[0057] When it is necessary to install the screen receiving plate 102, the rotating block 233 is rotated from the horizontal state to the vertical state, then the four fourth L-shaped plates 236 on the screen receiving plate 102 are placed on the top end of the four guide blocks 230 respectively, the rectangular grooves 237 on the fourth L-shaped plates 236 pass through the rotating block 233, then the rotating block 233 is rotated from the vertical state to the horizontal state, the first magnet 234 and the second magnet 235 are attracted to each other, the screen receiving plate 102 is limited, and the screen receiving plate 102 is convenient to install and disassemble.

[0058] As shown in Figure 12 The receiving assembly comprises two inclined plates 238, the top end of each of the two inclined plates 238 is fixedly connected with a surrounding edge 239, the surrounding edge 239 is provided with a drainage port 240, and the rinsing liquid tank 101 is provided with a second driving assembly.

[0059] When it is necessary to replace the rinsing liquid in the rinsing liquid tank 101, the screen receiving plate 102 is moved to the top end of the inclined plate 238 through the lifting assembly, then the second driving assembly is started to drive the two inclined plates 238 to move close to each other, so that the two inclined plates 238 are in contact, the rinsing liquid falling from the tissue screen 104 is received through the two inclined plates 238 and the surrounding edge 239, the received rinsing liquid can be discharged through the drainage port 240, the rinsing liquid in the main body is convenient to replace, and a long waiting time is avoided.

[0060] As shown in Figure 12As shown, the second drive assembly comprises a bottom plate 241 fixedly connected to the bottom end of the rinsing liquid tank 101, the connecting plate 225 is fixedly connected to the top end of the bottom plate 241, and the top end of the bottom plate 241 is fixedly connected with four limiting plates 242, one side of one of the limiting plates 242 is fixedly connected with a fifth L-shaped plate 243, the inner side of the fifth L-shaped plate 243 is fixedly connected with a third motor 244, the output end of the third motor 244 is fixedly connected with a bidirectional threaded rod 245, the bidirectional threaded rod 245 is rotatably penetrated in one of the limiting plates 242, and two moving plates 246 are threadedly connected on the bidirectional threaded rod 245. Two inclined plates 238 are fixedly connected to the moving plates 246 respectively, and a guide rod 247 is fixedly connected between the two limiting plates 242, and the two moving plates 246 are slidably connected to the guide rod 247.

[0061] When it is necessary to drive the two inclined plates 238, the third motor 244 is started, the output end of the third motor 244 drives the bidirectional threaded rod 245 to rotate, and the bidirectional threaded rod 245 drives the two inclined plates 238 to approach each other along the length direction of the guide rod 247, so that the two inclined plates 238 contact each other, the rinsing liquid falling from the tissue sieve screen 104 is received, and the received rinsing liquid can be discharged through the drain hole 240, so that the rinsing liquid in the main body can be replaced conveniently, and a long waiting time is avoided.

[0062] As shown in Figure 13 The inner bottom wall of the rinsing liquid tank 101 is provided with an inclined surface 248, one side of the rinsing liquid tank 101 is provided with a drain hole 249, the drain hole 249 is fitted with a rubber plug 250, and one side of the rubber plug 250 is fixedly connected with a pull ring 251.

[0063] When it is necessary to discharge the rinsing liquid in the rinsing liquid tank 101, the pull ring 251 is pulled to drive the rubber plug 250 to move away from the drain hole 249, and the rinsing liquid in the rinsing liquid tank 101 is discharged from the drain hole 249 under the action of the inclined surface 248.

[0064] As shown in Figure 1 - Figure 13 S1: Add appropriate rinsing liquid in the rinsing liquid tank 101, and ensure that enough rinsing liquid can cover all the tissue sections;

[0065] S2: Put the tissue sections into the tissue sieve screen 104 for bearing, and put the tissue sieve screen 104 into the sieve screen bearing plate 102;

[0066] S3: Adjust the height of the sieve screen bearing plate 102 through the lifting assembly, so as to control the immersion depth of the tissue sections in the rinsing liquid;

[0067] S3: Keep the rinsing liquid flowing through the circulating assembly to increase the contact area between the rinsing liquid and the tissue section.

[0068] The specific working process of the technical solution of the present application is as follows:

[0069] In use, an appropriate amount of rinsing liquid is poured into the rinsing liquid tank 101, then the tissue screen 104 is placed into the placing hole 103 on the screen receiving plate 102, the rotating block 233 is rotated from the horizontal state to the vertical state, then the four fourth L-shaped plates 236 on the screen receiving plate 102 are placed on the top ends of the corresponding four guide blocks 230 respectively, the rectangular grooves 237 on the fourth L-shaped plates 236 pass through the rotating block 233, the rotating block 233 is rotated again from the vertical state to the horizontal state, the first magnet 234 and the second magnet 235 are attracted to each other, and the screen receiving plate 102 is limited.

[0070] The second motor 227 is started, the output end of the second motor 227 drives the worm 228 to rotate, the worm 228 drives the two worm gears 224 and the screw rod 223 to rotate, the screw rod 223 drives the guide block 230 to move, the guide block 230 drives the screen receiving plate 102 to move downward along the length direction of the limiting rod 229, the tissue screen 104 is lowered into the rinsing liquid tank 101, the tissue sections in the tissue screen 104 are naturally floated in the rinsing liquid, the screen receiving plate 102 drives the vibration assembly to move downward at the same time in the lowering process, when the tissue screen 104 is lowered into the rinsing liquid tank 101, the multiple rotating shafts 201 and the fan blades 203 are located in the gap 204, the first circular gear 221 and the second circular gear 222 are engaged with each other.

[0071] The first motor 207 is started, the output end of the first motor 207 drives the second circular gear 222 and one of the rotating shafts 201 to rotate at the same time, through the action of the belt pulley 208 and the transmission belt 209, multiple rotating shafts 201 can be driven to rotate at the same time, multiple rotating shafts 201 drive the circular block 202 and the fan blades 203 to rotate at the same time, in the rotating process of the fan blades 203, the rinsing liquid in the rinsing liquid tank 101 can be driven from one end to the other end, and the rinsing liquid is circulated in turn, so that the rinsing effect of the rinsing liquid on the tissue sections in the tissue screen 104 is enhanced.

[0072] When the second circular gear 222 rotates, the second circular gear 222 can drive the first circular gear 221 and the rotating shaft 216 to rotate, the rotating shaft 216 drives the half gear 217 to rotate, the half gear 217 drives the rack plate 218 to reciprocate left and right, the rack plate 218 drives the knocking rod 219 to knock the fixed block 220, so that the fixed block 220, the connecting frame 214, the screen receiving plate 102 and the tissue screen 104 vibrate, and the tissue screen 104 can enhance the rinsing effect of the tissue slice in the vibration process, then, when the rinsing liquid in the rinsing liquid tank 101 is driven, the rinsing liquid can drive the rectangular plate 212 to rotate, the rectangular plate 212 can drive the tissue screen 104 to rotate in the placement hole 103, and the tissue screen 104 drives the tissue slice to rotate, so that the tissue section is cleaned more thoroughly, when it is necessary to replace the rinsing liquid in the rinsing liquid tank 101, the second motor 227 is started, the output end of the second motor 227 drives the worm 228 to rotate, the worm 228 drives the two worm gears 224 and the screw rod 223 to rotate, the screw rod 223 drives the guide block 230 to move, and the guide block 230 drives the screen receiving plate 102 to move upwards along the length direction of the limiting rod 229, so that the tissue screen 104 is moved to a position higher than the inclined plate 238.

[0073] Then, the third motor 244 is started, the output end of the third motor 244 drives the bidirectional threaded rod 245 to rotate, the bidirectional threaded rod 245 drives the two inclined plates 238 to approach each other along the length direction of the guide rod 247, so that the two inclined plates 238 contact each other, the rinsing liquid falling from the tissue screen 104 is received, and the received rinsing liquid can be discharged through the drain hole 240, the pull ring 251 is pulled, the pull ring 251 drives the rubber plug 250 to move away from the drain hole 249, and the rinsing liquid in the rinsing liquid tank 101 is discharged from the drain hole 249 through the action of the inclined surface 248.

[0074] The present application encompasses any alternatives, modifications, equivalent methods and solutions made on the essence and scope of the present application. In order to make the public have a thorough understanding of the present application, specific details are described in the following preferred embodiments of the present application, and the present application can also be completely understood without the description of these details for those skilled in the art. In addition, in order to avoid unnecessary confusion to the essence of the present application, well-known methods, processes, flows, elements and circuits are not described in detail.

[0075] The above is only the preferred embodiment of the present application, and it should be pointed out that, for ordinary skilled in the art, without departing from the principle of the present application, a number of improvements and refinements can also be made, and these improvements and refinements should also be regarded as the protection scope of the present application.

Claims

1. A device for rinsing antibodies from frozen tissue sections, comprising a rinsing solution tank and a sieve receiving plate, characterized in that, The screen receiving plate has multiple placement holes, and each of the multiple placement holes is equipped with a tissue screen. The rinsing liquid tank is equipped with a first driving component for driving the rinsing liquid. The screen receiving plate is equipped with a vibration component for vibrating the tissue screen. The rinsing liquid tank is equipped with a lifting component for raising and lowering the screen receiving plate. The lifting component is equipped with an installation component for installing the screen receiving plate. The rinsing liquid tank is equipped with a receiving component for receiving the rinsing liquid. The first drive assembly includes multiple rotating shafts, each of which rotatably passes through one end of a rinsing solution tank. A circular block is fixedly connected to one side of each rotating shaft, and multiple fan blades are fixedly connected to the outer surface of the circular block. A gap is provided between the multiple tissue sieves to cooperate with the multiple fan blades. A fixed plate is fixedly connected to one side of the rinsing solution tank, and the sides of the multiple rotating shafts away from the rinsing solution tank are rotatably connected to the fixed plate. A first L-shaped plate is fixedly connected to one side of the rinsing solution tank, and a first motor is fixedly connected to the inner side of the first L-shaped plate. One of the rotating shafts is fixedly connected to the output end of the first motor, and the output end of the first motor rotatably passes through the fixed plate. Pulleys are fixedly connected to the outer surfaces of the multiple rotating shafts, and the same transmission belt is provided between the multiple pulleys. The bottom ends of the multiple tissue sieves are symmetrically fixed with semicircular blocks at the front and back. A connecting shaft is fixedly connected between two corresponding semicircular blocks. A rectangular plate is rotatably connected to the outer surface of the connecting shaft. A blocking plate is fixedly connected to the bottom end of the tissue sieve, and the blocking plate is located between two rectangular plates.

2. The apparatus for rinsing antibodies from frozen tissue sections according to claim 1, characterized in that, The vibration assembly includes a connecting frame mounted on a screen receiving plate. A second L-shaped plate is fixedly connected to the front end of the connecting frame. A rotating shaft is rotatably connected between the rear end of the second L-shaped plate and the connecting frame. A semi-gear is fixedly connected to the outer surface of the rotating shaft. A rack plate is slidably connected to the front end of the connecting frame. The rack plate meshes with the rotating shaft. A striking rod is fixedly connected symmetrically to the left and right sides of the rack plate. Fixed blocks are fixedly connected symmetrically to the left and right sides of the front end of the connecting frame. A first circular gear is fixedly connected to the outer surface of the rotating shaft. A second circular gear is fixedly connected to the output end of the first motor.

3. The apparatus for rinsing antibodies from frozen tissue sections according to claim 2, characterized in that, The lifting assembly includes two screws, both of which are rotatably connected to the top of the rinsing liquid tank. Worm gears are fixedly connected to the outer surfaces of both screws. Two connecting plates are provided on the rinsing liquid tank. A third L-shaped plate is fixedly connected to one side of one of the connecting plates. A second motor is fixedly connected to the inner side of the third L-shaped plate. A worm gear is fixedly connected to the output end of the second motor. The worm gear rotatably passes through one of the connecting plates and cooperates with the two worm gears. Two limiting rods are fixedly connected to the top of the rinsing liquid tank. Guide blocks are provided on the outer surfaces of both the screws and the limiting rods. Two guide blocks are threaded onto the screws, and the other two screws are slidably connected to the limiting rods.

4. The apparatus for rinsing antibodies from frozen tissue sections according to claim 3, characterized in that, The mounting assembly includes four rectangular blocks, which are fixedly connected to four guide blocks respectively. The connecting frame is fixedly connected to the two front rectangular blocks. Each of the four rectangular blocks has a connecting post rotatably connected to its top. A rotating block is fixedly connected to the top of each connecting post. A first magnet is fixedly connected to one side of each rotating block. A second magnet is fixedly connected to the side of each guide block near the rectangular block. A fourth L-shaped plate is fixedly connected to each of the four corners of the screen receiving plate. The fourth L-shaped plate has rectangular grooves that cooperate with the rotating blocks.

5. The apparatus for rinsing antibodies from frozen tissue sections according to claim 4, characterized in that, The receiving component includes two inclined plates, each with a surrounding edge fixedly connected to its top. A drain outlet is provided on the surrounding edge. A second driving component is provided on the rinsing liquid tank.

6. The apparatus for rinsing antibodies from frozen tissue sections according to claim 5, characterized in that, The second drive assembly includes a base plate fixedly connected to the bottom of the rinsing liquid tank. A connecting plate is fixedly connected to the top of the base plate. Four limiting plates are fixedly connected to the top of the base plate. A fifth L-shaped plate is fixedly connected to one side of one of the limiting plates. A third motor is fixedly connected to the inner side of the fifth L-shaped plate. A bidirectional threaded rod is fixedly connected to the output end of the third motor. The bidirectional threaded rod rotatably passes through one of the limiting plates. Two movable plates are threadedly connected to the bidirectional threaded rod. Two inclined plates are fixedly connected to the movable plates respectively. A guide rod is fixedly connected between the two limiting plates. Both movable plates are slidably connected to the guide rod.

7. The apparatus for rinsing antibodies from frozen tissue sections according to claim 6, characterized in that, The inner bottom wall of the rinsing solution tank is provided with a slope, and a drain hole is provided on one side of the rinsing solution tank. A rubber plug is fitted into the drain hole, and a pull ring is fixedly connected to one side of the rubber plug.

8. A rinsing method applicable to the antibody rinsing apparatus for frozen sections as described in any one of claims 1-7, characterized in that, Includes the following steps: S1: Add an appropriate amount of rinsing solution to the rinsing solution tank to ensure that enough rinsing solution can cover all tissue sections; S2: Place the tissue slices into the tissue sieve for support, and then place the tissue sieve into the sieve receiving plate; S3: The height of the screen receiving plate is adjusted by the lifting component, thereby controlling the immersion depth of the tissue sections in the rinsing solution; S4: The rinsing solution is kept in a flowing state by the circulation component, which increases the contact area between the rinsing solution and the tissue section.

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