Cutting propagation method of semi-mangrove sea grapes

Abstract

The invention discloses a cutting propagation method for semi-mangrove sea grapes, and belongs to the technical field of semi-mangrove breeding. The cutting propagation method comprises the following steps that current-year robust tender branches of sea grape seed trees are sheared into cutting slips with oblique cutting lower notches and flat cutting upper notches, and at least one leaf is reserved on the upper notch of each cutting slip; the cut cutting slips are immediately immersed in a rooting solution and then taken out; the soaked cutting slips are inserted into a matrix, and the matrix around the cutting slips is sprayed with the rooting solution; and regularly watering the substrate around the cutting slips. The cuttage propagation method for the semi-mangrove sea grapes has the advantages of being convenient and easy to operate, low in cost, large in seedling size and high in transplanting adaptability.

Description

Technical Field

[0001] The invention relates to the technical field of semi-mangrove breeding, and in particular to a cutting propagation method for semi-mangrove sea grapes. Background Art

[0002] Sea grape is a kind of semi-mangrove small tree growing on the seaside, originating in America and Caribbean beaches, and is often used as a street tree in coastal cities or as a coastal windbreak tree species. As a Polygonaceae plant, sea grape has a large difference from the mangroves such as mangrove plants, sea moriaceae plants, white marina, and tung tree that are mainly distributed in China. Its leaves and fruits have high ornamental value, beautiful tree shape, strong disease and insect pest resistance, and large market potential. Since its introduction, it has been propagated and cultivated on a small scale in Yunnan, Guangdong, Hainan, Fujian, Taiwan and other southern regions in China, as a garden and garden ornamental plant or as a popular science education plant. Sea grape is generally expanded and propagated in the form of seeds and cuttings, but there is no detailed and available technology publication of the full process of sea grape cutting seedlings in China, and only one invention of sea grape tissue culture seedling cultivation is published (CN 114158478 B), there is a problem of high technical threshold and large facility demand, and the seedling cultivation process is imperfect, the tissue culture seedling is too small, lacks further cultivation in the nursery, and the transplanting adaptability is difficult to guarantee. Summary of the invention

[0003] The invention aims to provide a cutting propagation method for semi-mangrove sea grapes, aiming to have the advantages of convenient and simple operation, low cost, large seedling specifications and strong transplanting adaptability.

[0004] To achieve the above object, the present invention provides a cutting propagation method for semi-mangrove sea grapes, comprising the following steps:

[0005] Cut the strong young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, and retain at least one leaf on the upper incision of each cutting;

[0006] The cuttings are immediately immersed in a rooting solution and then taken out;

[0007] Inserting the soaked cuttings into a substrate, and pouring the rooting solution around the substrate;

[0008] The substrate around the cuttings is watered regularly.

[0009] In some embodiments, the rooting solution includes 300-700 mg / L naphthaleneacetic acid solution.

[0010] In some embodiments, the step of immediately immersing the cut cuttings in the rooting solution and then taking them out specifically includes:

[0011] The cuttings are immediately immersed in the naphthaleneacetic acid solution for 5 to 7 hours and then taken out and air-dried.

[0012] In some embodiments, the step of cutting the robust young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, wherein at least one leaf is retained on the upper incision of each cutting, specifically comprises:

[0013] The method for cutting the cuttings is to cut 2 to 3 internodes 0.8 to 1.2 cm apart upwards to form one cutting. The cutting length is 8 to 15 cm, and the top leaves of each cutting are retained.

[0014] In some embodiments, before the step of cutting the robust young branches of the current year's sea grape mother tree into cuttings with an oblique lower incision and a flat upper incision, wherein at least one leaf is retained on the upper incision of each cutting, the step further includes:

[0015] Wrap the strong young branches pruned from the sea grape mother tree with clean wet gauze, and remove the wet gauze after reaching the seedling raising site.

[0016] In some embodiments, the substrate is made of the following components in parts by weight: 10 to 30 parts of leaf mold, 10 to 30 parts of vermiculite, and 50 to 70 parts of sand.

[0017] In some embodiments, before the step of inserting the soaked cuttings into the substrate, the step further includes:

[0018] The substrate is uniformly moistened with a disinfectant solution 2 to 4 days in advance.

[0019] In some embodiments, the disinfection solution includes 1-5 g / L potassium permanganate solution.

[0020] In some embodiments, the step of inserting the soaked cuttings into a matrix and spraying the rooting solution on the matrix around the cuttings specifically includes:

[0021] A cutting pit with a depth of 3 to 5 cm is set in the center of the substrate, the soaked cuttings are inserted into the cutting pit, the substrate around the cuttings is compacted, and the rooting solution is sprinkled on the substrate around the cuttings.

[0022] In some embodiments, the step of regularly watering the substrate around the cuttings specifically includes:

[0023] After twenty days of irrigation with fresh water, the salinity of the irrigation water was increased by one thousandth each week until it reached 5 thousandths.

[0024] In the technical scheme of the present invention, by selecting the healthy and pest-free tender branches of the sea grape mother tree as the source of the cuttings, the best breeding effect can be guaranteed. When each cutting is cut, the lower incision is cut obliquely to increase the water absorption area, while the upper incision is cut flat, retaining at least one leaf to maintain photosynthesis, and ensuring the energy supply and growth vitality of the cuttings in the early stage of rooting. By immediately immersing the cuttings after cutting in the rooting solution, the rooting solution can stimulate the formation of adventitious roots, improve the rooting speed and success rate, and thus lay a solid foundation for subsequent healthy growth. By inserting the soaked cuttings into the pre-prepared matrix to ensure appropriate water and oxygen supply. After inserting the cuttings, the surrounding matrix is ​​sprinkled with an appropriate amount of rooting solution, which can further enhance the growth hormone concentration in the matrix and promote the rapid development of the root system. By regularly watering the cuttings and the surrounding matrix, a suitable humidity level is maintained to promote the healthy and rapid growth of the cuttings. In this way, a sea grape cutting seedling raising method with the advantages of convenient and simple operation, low cost, large seedling size and strong transplant adaptability is proposed. In summary, a sea grape cutting seedling raising method proposed in the present invention has the advantages of convenient and simple operation, low cost, large seedling size and strong transplant adaptability. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0026] Figure 1 It is a schematic diagram of the morphology of each stage in the cutting seedling raising process described in one embodiment of the present invention.

[0027] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0028] To make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0029] It should be noted that, in the embodiments, those without specifying specific conditions are carried out according to normal conditions or conditions recommended by the manufacturer. Those without specifying the manufacturer of reagents or instruments used are conventional products that can be purchased commercially. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes, and "A and / or B" is taken as an example, including schemes A, B, or A and B that meet the same time. In addition, the technical schemes between the various embodiments can be combined with each other, but must be based on the ability of ordinary technicians in the field to achieve. When the combination of the technical schemes is contradictory or cannot be achieved, it should be considered that the combination of such technical schemes does not exist, and is not within the scope of protection required by the present invention. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work, all belong to the scope of protection of the present invention.

[0030] Sea grape is a kind of semi-mangrove small tree growing on the seaside, originating in America and Caribbean beaches, and is often used as a street tree in coastal cities or as a coastal windbreak tree species. As a Polygonaceae plant, sea grape has a large difference from the mangroves such as mangrove plants, sea moriaceae plants, white marina, and tung tree that are mainly distributed in China. Its leaves and fruits have high ornamental value, beautiful tree shape, strong disease and insect pest resistance, and large market potential. Since its introduction, it has been propagated and cultivated on a small scale in Yunnan, Guangdong, Hainan, Fujian, Taiwan and other southern regions in China, as a garden and garden ornamental plant or as a popular science education plant. Sea grape is generally expanded and propagated in the form of seeds and cuttings, but there is no detailed and available technology publication of the full process of sea grape cutting seedlings in China, and only one invention of sea grape tissue culture seedling cultivation is published (CN 114158478 B), there is a problem of high technical threshold and large facility demand, and the seedling cultivation process is imperfect, the tissue culture seedling is too small, lacks further cultivation in the nursery, and the transplanting adaptability is difficult to guarantee.

[0031] In order to solve the above technical problems, the present invention provides a cutting propagation method for semi-mangrove sea grapes, comprising the following steps: cutting the strong young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, and retaining at least one leaf on the upper incision of each cutting; immediately immersing the cut cuttings in a rooting solution and then taking them out; inserting the soaked cuttings into a substrate, and sprinkling the rooting solution on the substrate around the cuttings; and regularly watering the substrate around the cuttings.

[0032] In the technical scheme of the present invention, by selecting the healthy and pest-free tender branches of the current year on the mother tree of sea grape as the source of the cuttings, the best breeding effect can be guaranteed. When each cutting is cut, the lower incision is cut obliquely to increase the water absorption area, while the upper incision is cut flat, retaining at least one leaf to maintain photosynthesis, and ensuring the energy supply and growth vitality of the cuttings in the early stage of rooting. By immediately immersing the cuttings after cutting in the rooting solution, the rooting solution can stimulate the formation of adventitious roots, improve the rooting speed and success rate, and thus lay a solid foundation for subsequent healthy growth. By inserting the soaked cuttings into the pre-prepared matrix to ensure appropriate water and oxygen supply. After inserting the cuttings, the surrounding matrix is ​​sprinkled with an appropriate amount of rooting solution, which can further enhance the growth hormone concentration in the matrix and promote the rapid development of the root system. By regularly watering the cuttings and the surrounding matrix, a suitable humidity level is maintained to promote the healthy and rapid growth of the cuttings. In this way, a sea grape cutting seedling raising method with the advantages of convenient and simple operation, low cost, large seedling size and strong transplant adaptability is proposed. In summary, a sea grape cutting seedling raising method proposed in the present invention has the advantages of convenient and simple operation, low cost, large seedling size and strong transplant adaptability.

[0033] It is understandable that the rooting solution can stimulate the formation of adventitious roots, accelerate the rooting speed and increase the rooting rate. They affect cell division and differentiation by simulating the effects of endogenous plant hormones, thereby promoting the generation of new roots. The rooting solution can be indolebutyric acid, indoleacetic acid, liquid rooting agent (available on the market), and naphthaleneacetic acid, and different rooting solutions have different adaptation concentrations. Specifically, in one embodiment, the rooting solution includes 300-700 mg / L naphthaleneacetic acid solution. In this way, the concentration range of the naphthaleneacetic acid solution of 300-700 mg / L is selected based on the needs of the special plant of sea grapes and to ensure the best rooting effect. Lower concentrations (such as 300 mg / L) are suitable for more sensitive or easy-to-root varieties, while higher concentrations (such as 700 mg / L) are helpful for those that are difficult to root or have more demanding growth conditions. By adjusting the specific concentration of the naphthaleneacetic acid solution, the rooting performance of the cuttings of sea grapes under different conditions can be optimized, so that it can obtain ideal breeding results in various environments. Different rooting solutions have different soaking times. Specifically, in one embodiment, the step of immediately immersing the cuttings in the rooting solution and then taking them out specifically includes: immediately immersing the cuttings in a naphthaleneacetic acid solution for 5 to 7 hours and then taking them out to dry. In this way, the longer soaking time allows the cuttings to fully absorb the naphthaleneacetic acid solution, thereby more effectively stimulating the formation of adventitious roots. The selection of this time period is based on experimental data and practical experience to ensure the best effect without causing excessive reaction. At the same time, after the cuttings have completed the immersion in the naphthaleneacetic acid solution, the next step is to take them out and dry them. The drying process is to remove excess solution on the surface of the cuttings to avoid unnecessary troubles in the subsequent cutting process, such as rotting caused by solution accumulation. The drying time can be adjusted according to actual conditions and needs.

[0034] In one embodiment, the step of cutting the strong young branches of the sea grape mother tree into cuttings with oblique lower incisions and flat upper incisions, and retaining at least one leaf on the upper incision of each cutting specifically includes: the method of cutting the cuttings is to cut 2 to 3 internodes staggered upward by 0.8 to 1.2 cm, for one cutting, the cutting length is 8 to 15 cm, and each cutting retains the top leaf. In this way, each cutting contains 2 to 3 internodes, and the internodes are staggered upward by 0.8 to 1.2 cm. Each cutting can have enough space to develop and will not be too crowded. The total length of each call number cutting should be between 8 and 15 cm, which is conducive to operation and sufficient to support the development of new roots. Each cutting retains at least one top leaf, which is conducive to photosynthesis, and thus helps the cutting to maintain life activities before taking root.

[0035] When the sea grape mother tree is far away from the seedling raising site, a temporary protective measure needs to be taken to ensure that the quality of the tender branches is not affected. To this end, in one embodiment, before the step of cutting the strong tender branches of the sea grape mother tree of the year into cuttings with an oblique lower incision and a flat upper incision, and retaining at least one leaf on the upper incision of each cutting, it also includes: wrapping the strong tender branches trimmed from the sea grape mother tree with clean wet gauze, and removing the wet gauze after arriving at the seedling raising site. It can be understood that the wet gauze can maintain a certain humidity to provide the necessary moisture for the tender branches, and at the same time be clean enough to avoid the introduction of any microorganisms or impurities that may contaminate the cuttings. By wrapping the tender branches tightly in wet gauze, a microenvironment can be formed, which helps to slow down the evaporation of water and maintain the freshness of the branches. When arriving at the seedling raising site, the wet gauze can be removed immediately, and the state of the tender branches can be checked to ensure that they are not affected by diseases or physical damage. Any damaged or unhealthy branches should be removed in time to avoid affecting the success rate of subsequent cuttings. In this way, even at a long distance, the impact of external factors on the branches can be reduced, high-quality cuttings can be obtained, and a good foundation for subsequent rooting and healthy growth can be laid.

[0036] It is understandable that the matrix can be any combination of the following components: peat soil, coconut husk (coconut fiber), perlite, vermiculite, compost, river sand, sawdust, ceramsite, peat, mineral fertilizer, and can be adapted and adjusted according to actual needs. Specifically, in one embodiment, the matrix is ​​made of the following components in parts by weight: 10 to 30 parts of leaf humus, 10 to 30 parts of vermiculite, and 50 to 70 parts of sand. It is understandable that leaf humus is rich in organic matter and various nutrients, such as nitrogen, phosphorus, potassium, etc., which are necessary for plant growth. At the same time, leaf humus also has good air permeability and water retention, which is conducive to providing a suitable microenvironment for the cuttings, providing the required nutrients for the cuttings, improving the air permeability and water retention of the soil, and promoting the growth and development of the cuttings. Vermiculite is a silicate mineral with a layered structure and has good water absorption, expansion and air permeability. When vermiculite absorbs water, its volume will expand several times, forming many tiny gaps. These gaps help increase the looseness and aeration of the soil, increase the looseness of the matrix, improve the aeration of the soil, and are conducive to the growth and development of the root system of the cuttings. At the same time, vermiculite can also serve as a reservoir of water and nutrients, providing continuous growth support for the cuttings. Sand is mainly composed of silicate minerals and has good drainage and air permeability. The particle size of sand is moderate, which can form a stable soil structure, prevent water accumulation in the matrix, and prevent the cuttings from rotting due to lack of oxygen in the roots. At the same time, sand can also provide a stable growth environment for the cuttings, which is conducive to the growth and expansion of its root system.

[0037] Since there are some microorganisms, especially bacteria or fungi, in the matrix, it is possible to cause adverse effects on the incision of the cuttings, such as infection, rot, etc. Therefore, before inserting the cuttings into the matrix, the microorganisms present in the matrix can be sterilized by the following disinfection methods: steam disinfection, solar disinfection, chemical disinfectants, hot water treatment, microwave treatment and chemical disinfection. Specifically, in one embodiment, the step of inserting the soaked cuttings into the matrix also includes: uniformly moistening the matrix with a disinfectant solution 2 to 4 days in advance. It is understandable that the matrix is ​​uniformly moistened with a disinfectant solution 2 to 4 days in advance. This pretreatment step helps to create a healthy and sterile growth environment, thereby improving the success rate of cuttings and the survival rate of cuttings. Among them, the disinfectant solution can be a broad-spectrum fungicide such as potassium permanganate, formalin (formaldehyde), bleaching powder (sodium hypochlorite), thiophanate-methyl, carbendazim, etc. As for the concentration of various disinfectant solutions, it can be adjusted according to specific circumstances. Specifically, in one embodiment, the disinfectant solution includes 1 to 5g / L potassium permanganate solution. Since potassium permanganate is a strong oxidant and its concentration is within 1 to 5 g / L, which is a usage concentration within the dilution range, it can effectively kill bacteria, fungi and some viruses. At the same time, it is easily decomposed under light. After using potassium permanganate for a period of time, it will be reduced to manganese dioxide or other low-valent manganese compounds and produce oxygen overflow, which will not affect the subsequent cuttings.

[0038] In order to ensure that the cuttings can be smoothly inserted into the matrix and obtain a good growth environment, and at the same time reduce the damage that may be caused to the cuttings by direct cutting, in one embodiment, the step of inserting the soaked cuttings into the matrix and sprinkling the rooting solution on the matrix around the cuttings specifically includes: setting a cutting pit with a depth of 3 to 5 cm in the center of the matrix, inserting the soaked cuttings into the cutting pit, compacting the matrix around the cuttings, and sprinkling the rooting solution on the matrix around the cuttings. It is understandable that the cutting pit can be dug using sterile tools. The depth of the cutting pit is selected to ensure that the cuttings can be stably inserted into the matrix without damaging its root system or trunk due to excessive insertion. By gently compacting the matrix around the cuttings so that the cuttings are in close contact with the matrix, it is beneficial to the growth of the root system and the absorption of nutrients. In this way, not only can the damage to the cuttings caused by direct cuttings be avoided, but also the cuttings can obtain a stable growth environment in the matrix, thereby improving their rooting speed and survival rate.

[0039] Since sea grapes like light and warm environment, in one embodiment, after inserting the soaked cuttings into the substrate and spraying the rooting solution around the cuttings, the step further includes: the seedling temperature of the cuttings is 22-32°C. In this way, the seedling temperature stabilized at 22-32°C combined with suitable light conditions can effectively promote root development and overall plant health, create an ideal growth environment for the cuttings of sea grapes, and improve the success rate of cutting propagation.

[0040] It should be noted that the cuttings of sea grapes should be protected from strong light and low temperature. In one embodiment, when the temperature of the seedling cultivation is lower than 8 degrees Celsius, a plastic shed is built above the cuttings for warmth. This arrangement increases the ambient temperature of the cuttings and protects the cuttings from damage due to the cold, thereby reducing the survival rate. In one embodiment, in summer seedling cultivation, a sunshade net needs to be built above the cuttings before they unfold their leaves. In this arrangement, the sunshade net blocks part of the sunlight, thereby reducing the burns to the leaves of the cuttings caused by the strong light of summer, and the subsequent withering or yellowing phenomenon, protecting the health of the leaves and ensuring their normal photosynthesis function.

[0041] In order to cultivate coastal environment or saline-alkali land seedlings, salinity acclimation should be carried out in the seedling stage to improve the recovery adaptation speed and survival rate after transplanting. In one embodiment, the step of regularly watering the matrix around the cuttings specifically includes: after irrigating with fresh water for twenty days, the weekly irrigation water increases the salinity of one thousandth point until five thousandths of salinity. It is understandable that in the early stage of the cuttings planting, fresh water is first used for irrigation for twenty days, in order to provide a stable, low-salinity growth environment for the cuttings, and promote the development and initial growth of its root system. After the fresh water irrigation stage ends, the salinity of the irrigation water is gradually increased, and the weekly irrigation water salinity is increased by one thousandth point until it reaches five thousandths of salinity, so that the cuttings are constantly adapted to the salinity, thereby achieving salinity acclimation. When the salinity of the irrigation water reaches the target value (such as five thousandths), this salinity level is maintained for irrigation until the cuttings are fully adapted to the high-salinity environment. In this way, the above-mentioned salinity acclimation measures can significantly improve the adaptability of sea grape seedlings to saline-alkali environments, thereby enhancing their recovery speed and survival rate after transplanting.

[0042] The technical solution of the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the following embodiments are only used to explain the present invention, and are not used to limit the present invention.

[0043] Embodiment 1 (spring planting)

[0044] Spring cuttings mainly focus on soil temperature, humidity, and temperature difference to ensure that the cuttings take root smoothly. The cutting depth, number of days for substrate pretreatment, watering frequency, cutting period, and heat preservation measures in the embodiment are all adopted to adapt to the climatic characteristics of spring seedling cultivation.

[0045] The following steps are involved:

[0046] S1. Make the matrix: Use a mixture of leaf humus, vermiculite and sand as the cutting matrix. The composition and weight ratio of the cutting matrix is ​​leaf humus: vermiculite: sand at 20:20:60. Mix the cutting matrix and put it into a flower pot with smooth drainage at the bottom. Use 0.2% potassium permanganate solution to evenly moisten the cutting matrix for disinfection 4 days before cutting.

[0047] S2. Select cuttings: Select healthy and disease-free young branches grown from excellent mother trees that are 5 years old.

[0048] S3. Preparation of cuttings: Cut the selected sea grape branches with clean and sharp pruning shears to avoid tissue damage. If the mother tree is far away from the nursery site, it should be wrapped with clean wet gauze during transportation. When arriving at the nursery site, rinse the branches with clean water and dry them in the shade. Cut them into 9 cm long cuttings with oblique cuts on the lower incision and flat cuts on the upper incision. One leaf should be retained on the upper incision of each cutting. After cutting, immediately immerse the cuttings in the prepared 500 mg / L naphthaleneacetic acid rooting solution, take them out after 5 hours, and dry them.

[0049] S4. Cutting: Cutting should be done after the temperature rises in late spring. Before cutting, water the cutting medium with water, but do not soak it, to form a better rooting environment; use a sterile glass rod to dig a 4 cm deep hole in the center of the medium as a cutting hole to avoid direct cutting and damage to the cuttings. Insert the cuttings into the hole and compact the matrix around the cuttings to make full contact with the cuttings. Water the outer matrix of the hole with rooting liquid, and then spray the leaves with water once;

[0050] S5. Management after cutting:

[0051] ① Temperature management: Sea grapes prefer warm climates. In southern my country, sea grape seedlings generally do not suffer from frost damage. However, if the temperature drops below 10 degrees Celsius for more than a week in the early stage of seedling cultivation, it is necessary to temporarily build a plastic shed to keep warm. The height of the plastic shed should be 1.5 meters.

[0052] ② Watering: The first twenty days after cuttings are the rooting period. During this period, you only need to water moderately to keep the soil moist. Too wet soil is not conducive to the growth and development of roots. After rooting, the amount of water should be determined according to the environment and soil humidity. Water once a week and ensure that the bottom of the flowerpot seeps smoothly to avoid excessive water causing root rot.

[0053] ③ Fertilization: Fertilize once every season, using fertilizers with high nitrogen content or balanced nutrition.

[0054] ④ Salinity acclimation: When cultivating seedlings for coastal environments or saline-alkali land, salinity acclimation should be carried out during the seedling stage to increase the recovery and adaptation speed and survival rate after transplantation. The specific measures are to use fresh water for irrigation in the first 20 days after cutting, and to increase the salinity of irrigation water by one thousandth every week until the salinity reaches 5 thousandths after 20 days of watering.

[0055] ⑤ Repotting: When the seedlings reach a height of 50 cm or 80 cm and there is still a need to expand the cultivation, they need to be repotted with pots with a diameter of 15 cm and 20 cm. Medium seedlings of 80 cm in height and large seedlings of 120 cm in height can be cultivated.

[0056] Embodiment 2 (summer planting)

[0057] The key points of summer cuttings are shading, cooling and humidity management to prevent the cuttings from losing water or withering due to high temperature or strong light, and to strengthen pest control. The cutting depth, substrate pre-treatment days, watering frequency and other measures in the embodiment are all adapted to the climate characteristics of summer seedling cultivation.

[0058] S1. Preparation of matrix:

[0059] Use a mixture of leaf humus, vermiculite and sand as the cutting medium. The cutting medium components and their weight ratios are leaf humus: vermiculite: sand at 20:20:60. Mix the cutting medium and put it into a flower pot with smooth drainage at the bottom. Use 0.2% potassium permanganate solution to evenly moisten the cutting medium for disinfection 2 days before cutting.

[0060] S2. Select cuttings:

[0061] Select healthy and disease-free young branches grown from excellent mother trees that are 5 years old.

[0062] S3. Preparation of cuttings:

[0063] Use clean, sharp pruning shears to cut the selected sea grape branches to avoid tissue damage. If the mother tree is far away from the nursery site, it should be wrapped in clean wet gauze during transportation. At the nursery site, rinse the branches with clean water and dry them in the shade. Cut them into 9 cm long cuttings with oblique lower incisions and flat upper incisions. One leaf should be retained on the incision of each cutting. In order to reduce the transpiration loss of the cuttings, the cuttings should be wrapped with plastic film. Excessive leaves should also be trimmed to the size of ears. After cutting, immediately immerse the cuttings in the prepared 500 mg / L naphthaleneacetic acid rooting solution, take them out after 5 hours, and dry them.

[0064] S4. Cutting: Before cutting, wet the cutting medium with water, but do not soak it, to form a better rooting environment; use a sterile glass rod to dig a 5 cm deep hole in the center of the medium as a cutting hole to avoid direct cutting to damage the cuttings, insert the cuttings into the hole, compact the matrix around the cuttings to make full contact with the cuttings. Water the outer matrix of the hole with rooting liquid, and then spray the leaves with water once;

[0065] S5. Management after cutting:

[0066] ① Light and temperature management: In summer, sea grape seedlings need to be cultivated in a semi-shaded environment under a sunshade net. In winter, they grow into half-year-old seedlings. If they encounter extreme low temperatures below 8 degrees Celsius for more than a week, they need to temporarily build a plastic shed to keep warm. The height of the plastic shed should be 1.5 meters.

[0067] ② Watering: The first twenty days after cutting is the rooting period. During this period, moderate watering is required to keep the soil moist. Too wet soil is not conducive to the growth and development of roots. After rooting, the amount of water should be determined according to the environment and soil humidity. In summer, the temperature is high and evaporation is fast. Watering is required twice a week, and ensure that the bottom of the flowerpot seeps smoothly to avoid excessive water. Spray the leaf surface with water once a day at noon in summer to create a cool and humid environment.

[0068] ③ Fertilization: Fertilize once every season, using fertilizers with high nitrogen content or balanced nutrition.

[0069] ④ Salinity acclimation: When cultivating seedlings for coastal environments or saline-alkali land, salinity acclimation should be carried out during the seedling stage to increase the recovery and adaptation speed and survival rate after transplantation. The specific measures are to use fresh water for irrigation in the first 20 days after cutting, and to increase the salinity of irrigation water by one thousandth every week until the salinity reaches 5 thousandths after 20 days of watering.

[0070] ⑤ Repotting: When the seedlings reach a height of 50 cm or 80 cm and there is still a need to expand the cultivation, they need to be repotted with pots with a diameter of 15 cm and 20 cm. Medium seedlings of 80 cm in height and large seedlings of 120 cm in height can be cultivated.

[0071] The above Example 1 was repeated 50 times to calculate the survival rate of each stage in the cutting seedling raising.

[0072] The above Example 2 was repeated 50 times to calculate the survival rate of each stage in the cutting seedling raising.

[0073] The various stages of the cuttings seedling raising are as follows Figure 1 The statistical results of the survival rates at various stages of different embodiments are shown in Table 1:

[0074] Table 1 Survival rate at each stage of different embodiments

[0075] Rooting rate Germination rate Leaf expansion rate Seedling survival rate (Example 1) Spring planting 88％ 86％ 80％ 76％ (Example 2) Summer planting 90％ 82％ 76％ 70％

[0076] In summary, the final seedling survival rate of the cuttings of the sea grapes planted in spring and summer was 70% or above, indicating that the sea grape cutting seedling raising method proposed in the present invention has the advantages of convenient and simple operation, low cost, large seedling specifications, and strong transplant adaptability.

Claims

1. A cutting propagation method for semi-mangrove sea grapes, characterized in that: The following steps are involved: Cut the strong young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, and retain at least one leaf on the upper incision of each cutting; The cuttings are immediately immersed in a rooting solution and then taken out; Inserting the soaked cuttings into a substrate, and pouring the rooting solution around the substrate; The substrate around the cuttings is watered regularly.

2. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: The rooting solution comprises 300-700 mg / L naphthaleneacetic acid solution.

3. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 2, characterized in that: The step of immediately immersing the cut cuttings in the rooting solution and then taking them out specifically includes: The cuttings are immediately immersed in the naphthaleneacetic acid solution for 5 to 7 hours and then taken out and air-dried.

4. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: The step of cutting the robust young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, wherein at least one leaf is retained on the upper incision of each cutting, specifically comprises: The method for cutting the cuttings is to cut 2 to 3 internodes 0.8 to 1.2 cm apart upwards to form one cutting. The cutting length is 8 to 15 cm, and the top leaves of each cutting are retained.

5. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: Before the step of cutting the robust young branches of the sea grape mother tree of the current year into cuttings with an oblique lower incision and a flat upper incision, wherein at least one leaf is retained on the upper incision of each cutting, the method further comprises: Wrap the strong young branches pruned from the sea grape mother tree with clean wet gauze, and remove the wet gauze after reaching the seedling raising site.

6. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: The matrix is ​​made of the following components in parts by weight: 10 to 30 parts of leaf mold, 10 to 30 parts of vermiculite and 50 to 70 parts of sand.

7. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1 or 6, characterized in that: Before the step of inserting the soaked cuttings into the substrate, the step further includes: The substrate is uniformly moistened with a disinfectant solution 2 to 4 days in advance.

8. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 7, characterized in that: The disinfection solution includes 1-5 g / L potassium permanganate solution.

9. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: The step of inserting the soaked cuttings into a matrix and spraying the matrix around the cuttings with the rooting solution specifically includes: A cutting pit with a depth of 3 to 5 cm is set in the center of the substrate, the soaked cuttings are inserted into the cutting pit, the substrate around the cuttings is compacted, and the rooting solution is sprinkled on the substrate around the cuttings.

10. The cutting propagation method of the semi-mangrove sea grape as claimed in claim 1, characterized in that: The step of regularly watering the matrix around the cuttings specifically includes: After twenty days of irrigation with fresh water, the salinity of the irrigation water was increased by one thousandth each week until it reached 5 thousandths.

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