Method for increasing chlorine ion content of tobacco leaves in chlorine-poor soil

Abstract

The invention discloses a method for increasing chlorine ion content of tobacco leaves in chlorine-poor soil, which is characterized in that paenibacillus polymyxa powder is uniformly mixed in a seedling culture medium during tobacco seedling culture, the dosage is 1000g / hm < 2 > (more than or equal to 50 billion / g), and other seedling culture management is the same as conventional management; during transplanting, paenibacillus polymyxa powder is sprayed, the dosage is 6000 g / hm < 2 > (larger than or equal to 50 billion / g), and other field management is the same as conventional management. According to the method, the chlorine ion content of the tobacco leaves can be increased by 0.2-0.3%, and the quality of the tobacco leaves is improved.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco nutrition, and in particular to a method for increasing the chloride ion content of tobacco leaves in chlorine-deficient soil. Background Art

[0002] The chlorine content of flue-cured tobacco in my country is relatively low. The chlorine content of flue-cured tobacco in each tobacco-growing area is lower than that of high-quality flue-cured tobacco in foreign countries. Less than 20% of the samples in China meet the chlorine content requirements for high-quality flue-cured tobacco, showing a trend of "low in the south and high in the north" among regions. Chlorine can promote the transportation of carbohydrates, help the synthesis of sugars, increase the sugar content in tobacco leaves, reduce the protein content, and increase the Schmuck value. The chlorine content in tobacco leaves is positively correlated with the total sugar, reducing sugar, and starch content of tobacco leaves. Within the appropriate range of chlorine application, the K2O / Cl ratio of tobacco leaves tends to be coordinated with the increase of chlorine application. Applying an appropriate amount of chlorine is not only beneficial to improving the resistance of tobacco plants, but also can make the chemical composition of tobacco leaves coordinated, with good aroma quality, soft texture, rich oil and elasticity, good combustibility, and increased yield. Xu Zicheng and other studies also showed that with the increase of chlorine content in tobacco leaves, the aroma quality, aroma volume, aftertaste, miscellaneous gas, combustibility, gray score and total score of tobacco smoking all showed a trend of increasing first and then decreasing. The chlorine content of flue-cured tobacco can improve the smoking quality of tobacco leaves within an appropriate range, which may be related to the fact that chlorine can promote the transport of carbohydrates in plants, help the synthesis of sugars, and make the chemical composition of tobacco leaves more coordinated. It is generally believed that it is more appropriate for tobacco leaves to contain 0.3% to 0.8% chlorine. At this time, the tobacco leaves are soft, elastic and oily, have good expansion, and low breakage rate, which can improve the shred rate of tobacco leaves; when the chlorine content is less than 0.3%, the tobacco leaves are thin, the contents are insufficient, the elasticity is poor, the leaves are dry and rough, easy to break, the shred rate is low, the filling of tobacco leaves is poor, and the finished cigarettes are prone to empty cigarettes, and the taste is spicy and bitter; Zhou Jiheng's research shows that a small amount of chlorine in tobacco leaves can keep the tobacco leaves hygroscopic, improve the storage quality of tobacco leaves, and reduce the cracking and damage of tobacco leaves.

[0003] Since chlorine is an active element that is easy to move, it is easy to be lost due to leaching after rainfall. At the same time, excessive application of potassium, calcium, magnesium, etc. in the soil can easily combine with chlorine in the soil, reducing the absorption of chlorine by tobacco plants. It can also easily combine with negatively charged ions (NO3 - 、NO2 - As a result, the chlorine content of tobacco leaves in most tobacco-growing areas in China is relatively low, and in some areas it is even lower than 0.1%, which to some extent restricts the improvement of the quality of tobacco leaves in my country. Summary of the invention

[0004] In order to increase the chloride ion content of tobacco leaves grown in chloride-deficient soil and improve the quality of tobacco leaves, the object of the present invention is to provide a method for increasing the chloride ion content of tobacco leaves grown in chloride-deficient soil.

[0005] The technical solution adopted by the present invention is as follows:

[0006] A method for increasing the chloride ion content of tobacco leaves in chloride-deficient soil comprises the following steps: uniformly mixing Paenibacillus polymyxa powder into a tobacco seedling medium during tobacco seedling cultivation, and performing other seedling cultivation management the same as conventional management; and spraying Paenibacillus polymyxa powder during transplanting, and performing other field management the same as conventional management.

[0007] The dosage of Bacillus polymyxa powder during seedling cultivation is 1000g / hm 2 .

[0008] The dosage of Bacillus polymyxa powder during transplanting is 6000g / hm 2 .

[0009] The number of live bacteria of Paenibacillus polymyxa in the powder is ≥5 billion / g.

[0010] The present invention also provides application of the above method in increasing the chloride ion content of tobacco leaves in chloride-deficient soil.

[0011] The present invention also provides application of the above method in improving the quality of tobacco leaves.

[0012] Chloride ions are anions, and anions are mainly actively absorbed by biofilm carriers. Paenibacillus polymyxa belongs to the genus Paenibacillus, a spore-forming Gram-positive bacterium that lives aerobically or facultatively anaerobically. Paenibacillus polymyxa can produce a variety of usable metabolically active substances, which can be divided into peptides, proteins, polysaccharides, etc. according to their structure. When applied to tobacco plants, they can quickly colonize in the rhizosphere and inside of the tobacco plants and form a "biofilm". Biofilm, also known as biofilm, refers to an organized bacterial community attached to the surface of living or inanimate objects and wrapped by bacterial extracellular macromolecules. The existence of biofilm not only acts as a barrier to create a stable internal environment for the life activities of cells, mediates the connection between cells and cells, and cells and matrix, but also undertakes the functions of material transport, transmembrane transmission of information and energy conversion, thereby promoting the absorption of anionic chloride ions by tobacco plants and increasing the chloride ion content of tobacco leaves. The formation of biofilm by bacteria is a dynamic process, which can be divided into four stages: the colonization stage of reversible bacterial adhesion, the aggregation stage of irreversible adhesion, the mature stage of biofilm, and the shedding and recolonization stage of bacteria. Adding Bacillus polymyxa powder to the seedling medium allows tobacco roots to carry Bacillus polymyxa, giving Bacillus polymyxa the conditions to occupy the colonization sites of tobacco roots first. Combined with the application of Bacillus polymyxa powder during transplanting, the field activity of Bacillus polymyxa is enhanced, forming a stronger biofilm.

[0013] The chlorine content of flue-cured tobacco in my country is relatively low, and the chlorine content of flue-cured tobacco in various tobacco-producing areas is lower than that of high-quality flue-cured tobacco in foreign countries, which restricts the improvement of the quality of tobacco leaves in my country to a certain extent. The present invention can increase the chloride ion content of tobacco leaves by 0.2 to 0.3 percentage points, thereby improving the quality of tobacco leaves. DETAILED DESCRIPTION

[0014] The following examples are provided to further illustrate the present invention, rather than to limit the present invention.

[0015] Example 1

[0016] Select the same chlorine-deficient field with medium fertility and set A: Paenibacillus polymyxa treatment (when growing tobacco seedlings, evenly mix Paenibacillus polymyxa powder into the seedling medium at a dosage of 1000g / hm2 2 , other seedling management is the same as conventional management. When transplanting, apply polymyxa bacillus powder at a dosage of 6000g / hm 2 , other field management is the same as conventional management, the number of viable Bacillus polymyxa in the powder is ≥5 billion / g) and B: control (same field, other fertilization and cultivation management measures are the same, except that Bacillus polymyxa powder is not mixed into the seedling medium, and Bacillus polymyxa powder is not applied during transplanting). There are two treatments, three repetitions, and a total of 6 plots. The arrangement order of the field plots is A1, B1 (first repetition) A2, B2 (second repetition) A3, B3 (third repetition). After the tobacco leaves are mature and baked, the first and eighteenth leaves (the first leaf starting from the top leaf of the tobacco plant) of each plot are taken to detect the chloride ion content. The detection method is the continuous flow method (YC / T162-2011).

[0017] Test results: The chloride ion content of tobacco leaves in A1 community was 0.32% (the first piece) and 0.55% (the eighteenth piece), the chloride ion content of tobacco leaves in B1 community was 0.12% (the first piece) and 0.25% (the eighteenth piece), the chloride ion content of tobacco leaves in A2 community was 0.33% (the first piece) and 0.56% (the eighteenth piece), the chloride ion content of tobacco leaves in B2 community was 0.13% (the first piece) and 0.26% (the eighteenth piece), the chloride ion content of tobacco leaves in A3 community was 0.31% (the first piece) and 0.54% (the eighteenth piece), and the chloride ion content of tobacco leaves in B3 community was 0.11% (the first piece) and 0.24% (the eighteenth piece).

[0018] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A method for increasing the chloride ion content of tobacco leaves in chlorine-deficient soil, characterized in that: When raising tobacco seedlings, evenly mix Bacillus polymyxa into the seedling medium, and apply Bacillus polymyxa when transplanting.

2. The method according to claim 1, characterized in that The dosage form of the polymyxa bacillus is powder.

3. The method according to claim 2, characterized in that The dosage of Bacillus polymyxa powder during seedling cultivation is 1000g / hm 2 .

4. The method according to claim 2, characterized in that: The dosage of Bacillus polymyxa powder during transplanting is 6000g / hm 2 .

5. The method according to claim 3 or 4, characterized in that: The number of live bacteria of Paenibacillus polymyxa in the powder is ≥5 billion / g.

6. Use of the method according to any one of claims 1 to 5 in increasing the chloride ion content of tobacco leaves in chloride-deficient soil.

7. Use of the method according to any one of claims 1 to 5 in improving the quality of tobacco leaves.