Specimen manufacturing method of large short-wing bamboo pest

Abstract

The invention discloses a method for manufacturing a specimen of a large short-wing bamboo-knot insect. The method specifically comprises the following steps: cutting, namely transversely cutting a dead bamboo-knot insect at an internode membrane position between a sixth abdominal node and a seventh abdominal node of the abdomen of the bamboo-knot insect; viscera are taken out from the incision, and the abdominal cavity of the polypide body is cleaned; the soaked insect bodies are placed in a dry place to be air-dried, after air drying, filler is stuffed into the notches to be shaped, and posture adjusting and displaying are conducted after shaping is completed; in conclusion, according to the manufacturing method of the bamboo knot insect specimen, the insect body is cut at the special position, so that the specimen is not greatly damaged, and the cleaning effect on the abdominal cavity of the insect body can be ensured.

Description

Technical Field

[0001] The invention belongs to the technical field of specimen making methods, and particularly relates to a specimen making method for a short-winged large stick insect. Background Art

[0002] Customs intercepts a large number of exotic pets every year, and stick insects are one of them. Stick insects have become a very popular pet insect in recent years. Therefore, illegally imported species often appear among the species intercepted by customs. Some types of stick insects need to be selected and made into specimens to increase customs staff's full understanding and research of such exotic species.

[0003] At present, when making stick insect specimens, the internal contents (internal organs) of the insect body are not removed. Instead, the specimen is simply shaped and treated with antiseptic treatment, such as adding mothballs. This simple antiseptic treatment has a poor antiseptic effect and is not conducive to the long-term collection of the specimens. In order to improve the antiseptic effect of the specimens, the internal contents of some medium-sized beetles are removed when making specimens. However, when removing the internal contents, the head and the middle part of the abdomen of the insect body are cut open, and then the contents are taken out from the incision in the head or the middle of the abdomen.

[0004] Cutting from the head can easily cause great damage to the stick insect and is not convenient for removing the contents, which is not conducive to the subsequent preparation and preservation of specimens. Although the contents can be removed cleanly when cutting from the middle of the abdomen, the toughness of the entire insect body is greatly affected when cutting from the middle of the abdomen, which will make the insect body fragile and easy to break in the later stage, which is not conducive to the subsequent preparation of specimens. Summary of the invention

[0005] The invention provides a method for preparing specimens of short-winged large stick insects, which solves the above problems.

[0006] The technical solution adopted by the present invention is a method for preparing a stick insect specimen, which specifically comprises the following steps:

[0007] Incision: Make a horizontal incision on the dead stick insect at the intersegmental membrane between the 6th and 7th abdominal segments;

[0008] Abdominal cavity cleaning: remove the internal organs through the incision, clean the worm body repeatedly with clean water, then pour sodium hydroxide solution into the worm body's abdominal cavity to soak and clean the residue in the worm body's abdominal cavity, then soak and clean the worm body again in alcohol;

[0009] Filling: The insect body cleaned with alcohol is air-dried, and then the filler is stuffed into the incision to shape it. After shaping, it is posed and displayed.

[0010] Preferably, the concentration of the sodium hydroxide solution during abdominal cavity cleaning is 1.8-2.2 mol / L, and the sodium hydroxide solution is soaked in the insect abdominal cavity for 4h-5h.

[0011] Preferably, the concentration of alcohol during abdominal cavity cleaning is 70%-75%, and the time for soaking the insect body in alcohol is 1-2 hours.

[0012] Preferably, the dead stick insects are frozen at -19°C to -20°C before incision. After freezing, the stick insects are placed in a softener, and 40%-50% alcohol is placed in the softener, and left for 24 hours to thaw.

[0013] Preferably, the filler used during filling is cotton.

[0014] Preferably, when adjusting the posture, place the filled insect body on plastic foam, cover the feet of the insect body with foam paper, cover the wings with sulfuric acid paper, and then use insect pins to fix the foam paper and sulfuric acid paper on the plastic foam. After adjusting the posture, wait 2-3 days to fix the posture. After the posture is fixed, remove the insect pins, foam paper and sulfuric acid paper.

[0015] Preferably, during display, insect pins are used to fix the insect specimen in a specimen box for display.

[0016] The beneficial effects of the present invention are:

[0017] The invention discloses a method for preparing a stick insect specimen. When processing the stick insect specimen, a transverse incision is made at the position of the intersegmental membrane between the sixth and seventh abdominal segments of the abdomen, and the internal organs are taken out through the incision position. Because the sixth and seventh abdominal segments are close to the end of the abdomen, the incision has little effect on the overall flexibility of the specimen, and the incision can also be used to conveniently take out the contents at various positions of the insect body, including the thorax, abdomen and tail. After the contents are taken out through the incision, a sodium hydroxide solution is poured through the incision to soak and further clean the residues in the abdominal cavity of the insect body. The stick insect specimen preparation method disclosed by the invention performs incision processing on the insect body at a special position, which not only does not cause significant damage to the specimen, but also can ensure the cleaning effect of the abdominal cavity of the insect body. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 A flowchart of the method for making the present invention;

[0019] Figure 2 This is a schematic diagram of the operation corresponding to step 1 of the present invention;

[0020] Figure 3 This is a schematic diagram of the operation corresponding to step 2 of the present invention;

[0021] Figure 4This is a schematic diagram of the operation corresponding to step 2 of the present invention;

[0022] Figure 5 This is a schematic diagram of the operation corresponding to step 3 of the present invention;

[0023] Figure 6 This is a schematic diagram of the operation corresponding to step 6 of the present invention;

[0024] Figure 7 This is a schematic diagram of the operation corresponding to step 5 of the present invention. DETAILED DESCRIPTION

[0025] The present invention is described in detail below in conjunction with the accompanying drawings and specific implementation methods. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and cannot be used to limit the protection solution of the present invention.

[0026] The order Stick Insects is the most slender insect species, with an adult body length of 10 cm and up to 64 cm. Because they often lean over bamboo branches, their body color and shape are difficult to distinguish from the bamboo branches, and their mimicry skills are very superb, almost indistinguishable from the real thing, so they are called stick insects; many stick insects have become quite popular pet insects in recent years. Stick insect specimens are the most intuitive way to show the public the morphology of stick insects. Stick insect specimen making is a technology that reproduces the image of stick insects when they are alive. Stick insect specimens can restore the shape of stick insects when they are alive, making them lifelike and permanently preserved; stick insect specimens can be used for popular science teaching, can be preserved and exhibited for a long time, provide reference for the identification and classification of stick insects, and can also be used as handicrafts for players to collect.

[0027] When traditional stick insect specimens are made, the internal contents (internal organs) of the insect body are not removed. Instead, the insect body is simply shaped and treated with antiseptic treatment, such as adding mothballs. This simple antiseptic treatment has a poor antiseptic effect and is not conducive to the long-term collection of specimens. In order to improve the antiseptic effect of specimens, the internal contents of some medium-sized beetles are removed when specimens are made. However, when removing the internal contents, the head and abdomen of the insect body are cut open, and the contents are taken out from the incision on the head or abdomen.

[0028] Cutting from the head can easily cause great damage to the stick insect and is not convenient to clean out the contents, which is not conducive to the subsequent preparation and preservation of specimens. Although the contents can be cleaned out when taking out from the middle of the abdomen, the toughness of the entire insect body is greatly affected when the incision is made from the middle of the abdomen. If the incision is made between the 3rd and 4th nodes, it can also be cleaned, but the incision between the 3rd and 4th nodes has a greater impact on the toughness of the entire insect body, which will cause the insect body to be fragile and easy to break in the later stage, which is not conducive to the subsequent preparation of specimens.

[0029] Example 1

[0030] A method for preparing a specimen of a short-winged large stick insect comprises the following steps: Figure 1 The flowchart of the whole production method is as follows:

[0031] Incision: Figure 2 As shown, a newly dead stick insect was taken, the overall length of the stick insect was 13 mm, and a horizontal incision was made at the intersegmental membrane between the 6th and 7th abdominal segments of the stick insect's abdomen, and the incision length was about 1 cm;

[0032] Abdominal cleaning: Figure 3 As shown, use pointed tweezers to penetrate deep into the incision and take out the internal organs, including the contents of the chest, abdomen and tail. Then rinse the abdominal cavity of the insect body with clean water for 4 times, and then use a syringe to fill the insect body with 2 mol / L sodium hydroxide solution from the incision. Use sodium hydroxide solution to soak the abdominal cavity residue for 4 hours to remove the remaining internal tissues. Then pour out the sodium hydroxide solution in the abdominal cavity, and then soak the insect body in a 75% alcohol solution for 1 hour. The alcohol can clean up the contents dissolved in sodium hydroxide; as shown in FIG. Figure 4 As shown, the abdomen can be squeezed during the soaking period. The role of squeezing is to remove the residual substances after the sodium hydroxide dissolves in the body. Note that the concentration of sodium hydroxide solution is 1.8-2.2 mol / L, which has the best effect, that is, it can clean the abdominal cavity without eroding the body wall. The role of sodium hydroxide is to dissolve the visceral residues in the abdominal cavity.

[0033] The steps for preparing 2 mol / l sodium hydroxide solution are as follows:

[0034] 1) Calculate the mass of sodium hydroxide required. Since the molar mass of sodium hydroxide is 40 g / mol, the weight of 2 moles of sodium hydroxide is 80 g.

[0035] 2) Use an electronic balance to accurately weigh 80 grams of sodium hydroxide and place it in a beaker;

[0036] 3) Add an appropriate amount of distilled water to the beaker and stir with a glass rod until the sodium hydroxide is completely dissolved;

[0037] 4) Transfer the dissolved solution to a 1-liter volumetric flask, being careful not to spill it outside to avoid affecting the concentration;

[0038] 5) Wash the beaker and glass rod with distilled water, pour the washing solution into the volumetric flask, and continue to add distilled water until the scale line of the volumetric flask is reached to ensure that the solution volume is 1 liter;

[0039] 6) Shake the solution to ensure that all ingredients are fully mixed;

[0040] The correct ratio of 75% alcohol refers to the matching value of alcohol and water, which is usually 3:1. 750 ml of absolute alcohol and 250 ml of distilled water can make the alcohol concentration become 75%;

[0041] Filling: Place the insect body cleaned with alcohol in a dry place to air dry, and then fill it with cotton or other fillings through the cut to shape it. Figure 5 As shown, the air-drying treatment method for insects can not only adopt natural air drying or heating drying, but also adopt other air-drying treatment methods. As long as the insects can be dried and dehydrated, the specific air-drying treatment method can be selected according to actual needs, and such changes will not affect the protection scope of this application;

[0042] Posture: Figure 6 As shown, first place the filled insect body on the plastic foam, cover the foot of the insect body with foam paper, then fix the foam paper on the plastic foam with insect pins, then cover the wings with sulfuric acid paper, and also fix the sulfuric acid paper on the plastic foam with insect pins. After the posture is adjusted, wait for 3 days to fix the posture. After the posture is fixed, remove the sulfuric acid paper and insect pins.

[0043] Display: As Figure 7 As shown, after the posture is fixed, use an insect pin to put the insect specimen into the specimen box for display. An insect pin can be inserted at the mesothorax to fix it. Use No. 5 insect pin. The abdomen and ventral surface of the thorax can be fixed to the specimen box with white glue (depending on the situation, if it is taken out later, it will not be fixed).

[0044] Insect pins are essential tools for making insect specimens, because they are used to fix the position of insects when making various forms of specimens. Different insect pins must be used for insects of different sizes. According to the thickness and length of the insect pins, they can be divided into 7 types: 00, 0, 1, 2, 3, 4, and 5. The length of the 0-5 needle is 38.45 mm, the diameter of the 0 needle is 0.3 mm, and the diameter increases by 0.1 mm for each increase of 1. The 5 needle is the thickest, with a diameter of 0.8 mm. The method of using insect pins is generally to insert the pin in the center of the insect body. In order to preserve the classification characteristics from damage and standardize the insertion of insect specimens, the insertion position of insects of different groups has the same requirements; the insertion of coleoptera is in the inner front of the right elytra, so that the needle passes between the right middle leg and the hind leg. The insertion of stink bugs is slightly to the right of the middle of the scutellum. Orthoptera insects such as locusts and katydids should be inserted behind the pronotum, to the right of the dorsal midline. Lepidoptera, Hymenoptera, Homoptera, etc. are inserted in the center of the mesothorax.

[0045] The plastic foam board used for posture fixation should be 2-3 cm thick, 60 cm long and 40 cm wide. Local materials can be used according to local conditions. The foam boards used for general packaging can be used, which is economical and can save materials.

[0046] When the posture is fixed, the covering paper can be sulfuric acid paper or other translucent materials. As long as the insect wings can be seen after covering, the specific type of translucent covering paper can be selected according to actual needs. Such changes will not affect the protection scope of this application.

[0047] Finally, the box for displaying the specimens is made of foamed plastic. In actual application scenarios, the box can be made not only of foamed plastic, but also of other materials. As long as the production and display of insects are not affected and the cost is reasonable, the specific material can be selected according to actual needs. Such changes will not affect the scope of protection of this application. In addition, the insect specimen box also has the advantages of easy processing, not easy to damage, low cost, light weight, shockproof, moisture-proof, and convenient transportation.

[0048] Example 2

[0049] A method for preparing a specimen of a short-winged large stick insect comprises the following steps:

[0050] Cut: Take out the dead stick insects stored under frozen conditions, put the frozen stick insects into a softener, and put 40% alcohol in the softener to prevent mold. Then put it for 24 hours to thaw. After thawing, cut it horizontally at the intersegmental membrane between the 6th and 7th abdominal segments of the stick insect. The size of the selected stick insect is 13cm, and the length of the cut is 1cm. The dead stick insects should be stored at -20℃ for a long time. If they have just died, they can be treated at room temperature without the softening step.

[0051] The softener is a special glass container or cylinder with a movable support plate in the middle and a hole. When using it, pour a small amount of clean water into the cylinder, and then add alcohol to the water to prevent the specimen from growing mold; the insect body collected and stored in the wild or frozen is hard and brittle. If it is handled immediately, the specimen will be damaged, so it must be softened with a softener before making;

[0052] Abdominal cavity cleaning: Use pointed tweezers to penetrate deep into the incision and remove the internal organs, including the contents of the chest, abdomen and tail. Then rinse the abdominal cavity of the insect repeatedly with clean water, and then use a syringe to fill the insect body with 2.2mol / L sodium hydroxide solution from the incision. Soak the abdominal cavity residues with sodium hydroxide solution for more than 4 hours to remove the residual internal tissues. Then pour out the sodium hydroxide solution in the abdominal cavity, and then soak the insect body in a 70% alcohol solution for 1 hour. During this period, the abdomen can be squeezed so that the residual substances in the abdominal cavity are completely dissolved by sodium hydroxide.

[0053] Alcohol is used here because it can dissolve fat and has strong permeability. Alcohol is an organic compound, the main component of which is ethanol, and it also contains acetaldehyde, water, tar, impurities and other components. It has strong permeability and can enter the bacteria to coagulate bacterial proteins, thereby achieving the purpose of disinfection. In addition, alcohol also has a bactericidal effect and can be used to disinfect the surface of the skin or the surface of objects;

[0054] Filling: Place the insect body cleaned with alcohol in a dry place to air dry, and then fill it with cotton or other filling materials through the incision to shape it. Figure 5 As shown;

[0055] Posture: Figure 6 As shown, first place the filled insect body on the plastic foam, cover the foot of the insect body with foam paper, then fix the foam paper on the plastic foam with insect pins, then cover the wings with sulfuric acid paper, and also fix the sulfuric acid paper on the plastic foam with insect pins. After the posture is adjusted, wait for 3 days to fix the posture. After the posture is fixed, remove the sulfuric acid paper and insect pins.

[0056] Display: As Figure 7 As shown, after the posture is fixed, use an insect pin to put the insect specimen into the specimen box for display.

[0057] In summary, the present invention provides a method for preparing stick insect specimens. When processing the stick insect specimen, a transverse incision is made at the position of the intersegmental membrane between the 6th and 7th abdominal segments of the abdomen, and the internal organs are removed through the incision. Because the 6th and 7th abdominal segments are close to the end of the abdomen, the incision has little effect on the overall flexibility of the specimen, and the contents of the insect body including the thorax, abdomen and tail can be conveniently removed through the incision. Finally, the abdominal cavity of the insect body can be cleaned through the incision. The stick insect specimen preparation method disclosed in the present invention performs incision processing on the insect body at a special position. Firstly, it will not cause significant damage to the specimen, and secondly, it can ensure the cleaning effect of the abdominal cavity of the insect body. Figure 7 The figure shows the stick insect specimen finally obtained by the preparation method of the present invention. It can be seen that the damage to the insect body is very small, and the stick insect specimen finally obtained has a lifelike morphology.

[0058] The embodiments described above are only preferred specific implementation methods of the present invention, and the protection scope of the present invention is not limited thereto. Any simple changes or equivalent replacements of the technical solutions that can be obviously obtained by any technician familiar with the field within the technical scope disclosed in the present invention belong to the protection scope of the present invention.

Claims

1. A method for preparing a stick insect specimen, characterized in that: The specific steps include: Incision: Make a horizontal incision on the dead stick insect at the intersegmental membrane between the 6th and 7th abdominal segments; Abdominal cavity cleaning: remove the internal organs through the incision, clean the worm body repeatedly with clean water, then pour sodium hydroxide solution into the worm body's abdominal cavity to soak and clean the residue in the worm body's abdominal cavity, then soak and clean the worm body again in alcohol; Filling: The insect body cleaned with alcohol is air-dried, and then the filler is stuffed into the incision to shape it. After shaping, it is posed and displayed.

2. A method for preparing a stick insect specimen according to claim 1, characterized in that: When cleaning the abdominal cavity, the concentration of the sodium hydroxide solution is 1.8-2.2 mol / L, and the sodium hydroxide solution is soaked in the abdominal cavity of the insect for 4h-5h.

3. A method for preparing a stick insect specimen according to claim 1, characterized in that: The concentration of alcohol during abdominal cavity cleaning is 70%-75%, and the insect body is soaked in alcohol for 1-2 hours.

4. A method for preparing a stick insect specimen according to claim 1, characterized in that: Before making an incision, the dead stick insects are frozen at -19°C to -20°C. After freezing, the stick insects are placed in a softener, and 40%-50% alcohol is placed in the softener, and left for 24 hours to thaw.

5. The method for preparing a stick insect specimen according to claim 1, characterized in that: The stuffing used when filling is cotton.

6. A method for preparing a stick insect specimen according to claim 1, characterized in that: When adjusting the posture, place the filled insect body on the plastic foam, cover the feet of the insect body with foam paper, cover the wings with sulfuric acid paper, and then use insect pins to fix the foam paper and sulfuric acid paper on the plastic foam. After adjusting the posture, wait 2-3 days to fix the posture. After the posture is fixed, remove the insect pins, foam paper and sulfuric acid paper.

7. A method for preparing a stick insect specimen according to claim 6, characterized in that: During display, insect pins are used to fix the insect specimens in the specimen box for display.

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