Pesticide composition containing pydiflumetofen

Abstract

The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing pydiflumetofen. The invention relates to a pesticide composition containing pydiflumetofen. The pesticide composition is prepared by compounding pydiflumetofen and matrine or phenazino-1-carboxylic acid. The pydiflumetofen and the sophocarpidine or the phenazino-1-carboxylic acid are reasonably compounded to be used for preventing and treating the crop diseases, and compared with a single component, the pesticide composition has the advantages that the disease prevention and treatment effect can be improved, the pesticide dosage is effectively reduced, the drug resistance risk is reduced, and the development speed of the drug resistance of the crop diseases is delayed; a thought can be provided for development and scientific use of medicaments.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition containing fluopicolide. Background Art

[0002] Fluopicolide is the fourth succinate dehydrogenase inhibitor (SDHI) fungicide launched by Syngenta after pyraclostrobin, fluopicolide and fenpropifluanid. The mechanism of action is as follows: Fluopicolide is a pathogen respiration inhibitor, which inhibits the function of mitochondria by interfering with the tricarboxylic acid cycle on complex II of the respiratory electron transport chain, preventing them from producing energy, inhibiting the growth of pathogens, and ultimately leading to their death. Fluopicolide has the characteristics of broad spectrum, systemicity and high efficiency, and can be used to prevent and control diseases caused by various pathogens such as Alternaria and Fusarium. It has been registered for use on crops such as wheat, rapeseed, peanuts, strawberries, grapes and tomatoes in my country. In addition, fluopicolide also has a nematicidal effect, and its effect on nematodes is mainly reflected in its nematicidal activity as an SDHI fungicide.

[0003] Matrine is a pyridine derivative alkaloid with hexahydropyridine as the parent, which is prepared by extracting the dried roots, plants and fruits of the leguminous plant Sophora flavescens through organic solvents such as ethanol. The prior art discloses that matrine has insecticidal, fungicidal, herbicidal and nematicidal activities.

[0004] The main component of shinazine is phenazine-1-carboxylic acid (PCA), a secondary metabolite produced by Pseudomonas aeruginosa M18, a melon rhizosphere biocontrol bacterium. It is a green pesticide of microbial origin. Shinazine belongs to the aromatic heterocyclic phenazine class of compounds, which have antibacterial, antitumor and antiparasitic activities. There are at least two mechanisms for the antibacterial activity of shinazine: first, phenazine compounds produce toxic superoxide ions and hydrogen peroxide during the reduction process in pathogenic bacteria cells, which can oxidize glutathione and transferrin to produce highly cytotoxic hydroxyl free radicals; second, phenazine compounds can be reduced by NADH and become intermediates in electron transfer, disrupting the normal redox homeostasis in cells, affecting energy production, and thus inhibiting microbial growth. At present, the deeper antibacterial mechanism of shinazine is still unclear.

[0005] In the agricultural production process, pesticide resistance is an issue that cannot be avoided. Pesticide resistance refers to the fact that after long-term exposure to pesticides, harmful biological targets such as pathogens and pests and their offspring will gradually develop resistance to pesticides, resulting in reduced or no efficacy. In the actual growth process, the problem of pesticide resistance is particularly prominent when a single variety of pesticides is used for a long time. In addition, farmers blindly increase the amount of pesticides used and the number of times they apply pesticides in order to improve the effectiveness of prevention, which not only increases the cost of prevention and control, but also exacerbates the generation and accumulation of pesticide resistance.

[0006] The rational combination of pesticides with different mechanisms of action can reduce the selection pressure of a single pesticide, thereby slowing the speed at which pests and diseases develop resistance. In addition, the rational combination of pesticides can also improve the effectiveness of prevention and control and expand the types of prevention and control. It is an effective way to comprehensively manage existing resistant pests and diseases. Summary of the invention

[0007] The purpose of the present invention is to provide a pesticide composition containing pentothiopyraclostrobin to solve the following problems: Long-term use of a single type of pesticide has particularly prominent problems with pesticide resistance. In addition, farmers blindly increase the dosage and frequency of pesticide application in order to improve the control effect, which not only increases the cost of prevention and control, but also exacerbates the generation and accumulation of pesticide resistance.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A pesticide composition containing fluopicolide, which is prepared by compounding fluopicolide with matrine or sinocycline.

[0010] Furthermore, the compounding mass ratio of fluopicolide to matrine is 1-15:15-1.

[0011] Furthermore, the composite mass ratio of fluopicolide hydroxylamide to sinocycline is 1-3:7-1.

[0012] A pesticide preparation comprising the pesticide composition, wherein the mass of the pesticide composition accounts for 0.1-80% of the total mass of the pesticide preparation, and the rest are auxiliary components permitted in agriculture.

[0013] Furthermore, the mass of the pesticide composition accounts for 2-65% of the total mass of the pesticide formulation.

[0014] Furthermore, the agriculturally acceptable auxiliary ingredients are selected from one or more of wetting agents, dispersants, disintegrants, emulsifiers, solvents, carriers, thickeners, defoamers, synergists and preservatives.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The invention uses fluopicolide and matrine or sinocycline to prevent and treat crop diseases after being reasonably compounded. Compared with a single component, the disease prevention and treatment effect can be improved, the amount of pesticide used can be effectively reduced, the risk of drug resistance can be reduced, and the development speed of drug resistance of crop diseases can be slowed down, which can provide ideas for the development and scientific use of agents. DETAILED DESCRIPTION

[0017] The present invention is further described below by way of examples. It should be noted that the examples of the present invention are only used to illustrate the present invention, rather than to limit the present invention.

[0018] Example: Indoor biological activity test of compounded drugs

[0019] 1. Test subjects

[0020] Soybean cyst nematode (Heteroderaglycines). Collect soil samples from plots severely damaged by soybean cyst nematodes, separate cysts by floating method, observe under a microscope, and pick out plump brown cysts. Add 0.5% sodium hypochlorite solution, disinfect the surface for 2 minutes, wash with sterile water 3 times, incubate in the dark at 26°C, collect second-instar larvae of the same age, and prepare a soybean cyst nematode suspension of 500 larvae / mL with sterile water.

[0021] 2. Test reagents

[0022] 98% fluopicolide technical (Syngenta Nantong Crop Protection Co., Ltd.), 10% matrine technical (Inner Mongolia Zhongnong Biochemical Technology Co., Ltd.), 95% sinocycline technical (Shanghai Nong Le Biological Products Co., Ltd.)

[0023] 3. Test method: (Refer to "NT / Y 1833.1-2009 Guidelines for Indoor Bioassay Tests of Pesticides - Nematicides Part 1 - Tests for Inhibition of Plant Pathogenic Nematodes - Insect Immersion Method")

[0024] After the test agent is dissolved, it is diluted with 0.1% Tween-80 aqueous solution to a single-dose stock solution with an active ingredient concentration of 10000 mg / L; then multiple groups of ratios are set up, and each single-dose stock solution and each group of mixed ratios are further diluted with 0.1% Tween-80 aqueous solution to prepare 5 mass concentration gradients for use.

[0025] 1mL of the drug solution was added dropwise to a sterile 24-well biochemical test plate, and then 1mL of soybean cyst nematode suspension was added to each well, and the treatment with 0.1% Tween-80 aqueous solution was used as a blank control. Each treatment was repeated 5 times, and the plates were covered and placed at 26°C for constant temperature culture. After 24 hours, 1mL of the mixed solution was taken from each treatment to observe the death of nematodes under a dissecting microscope. 200 nematodes were observed in each repetition, and the total number of nematodes investigated and the number of dead nematodes were recorded. The nematodes were stiff and could not bend and move when touched with a bamboo needle, which was considered dead; based on the survey data, the mortality rate and corrected mortality rate of each treatment were calculated.

[0026]

[0027] In the above formula: P--mortality rate, unit is %; K--number of dead insects; N--total number of insects treated.

[0028]

[0029] In the above formula: P1--corrected mortality rate, unit is %; Pt --Treatment mortality rate, in %; P0--blank control mortality rate, in %.

[0030] 4. Data Analysis

[0031] DPS software was used to perform regression analysis on the logarithmic values ​​of the concentration of each treatment agent and the corrected mortality probability value of each treatment to calculate the LC of each treatment agent. 50 , and the co-toxicity coefficient (CTC value) of the mixture was calculated according to Sun Yunpei's method.

[0032] 5. Efficacy evaluation

[0033] The synergistic effect of the drug was evaluated based on the calculated co-toxicity coefficient (CTC). CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Table 1-2.

[0034] Table 1 Results of toxicity test of fluopicolide and matrine against soybean cyst nematode (24h)

[0035]

[0036] As can be seen from Table 1, within the mass ratio range of 1-15:15-1, the co-toxicity coefficient of fluopicolide and matrine to soybean cyst nematode was greater than 120, showing a synergistic effect.

[0037] Table 2 Results of toxicity test of fluopicolide and sinocycline against soybean cyst nematode (24h)

[0038]

[0039] It can be seen from Table 2 that within the mass ratio range of 1-3:7-1, the co-toxicity coefficient of fluopicolide and sinocycline against soybean cyst nematode was greater than 120, showing a synergistic effect.

[0040] In summary, the present invention rationally compounds fluopicolide with matrine or sinocycline for use in preventing and controlling crop diseases. Compared with a single component, the disease prevention and control effect can be improved, the amount of pesticides used can be effectively reduced, the risk of drug resistance can be reduced, and the development speed of drug resistance in crop diseases can be slowed down, which can provide ideas for the development and scientific use of agents.

Claims

1. A pesticide composition containing pentopyralid, characterized in that: The pesticide composition is prepared by compounding pentocyclanil and matrine or sinocycline.

2. The pesticide composition according to claim 1, characterized in that The composite mass ratio of fluopicolide to matrine is 1-15:15-1.

3. The pesticide composition according to claim 1, characterized in that The composite mass ratio of fluopicolide to sinocycline is 1-3:7-1.

4. A pesticide preparation comprising the pesticide composition according to claim 1, characterized in that: The mass of the pesticide composition accounts for 0.1-80% of the total mass of the pesticide preparation, and the rest are auxiliary components permitted in agriculture.

5. The pesticide formulation according to claim 4, characterized in that: The mass of the pesticide composition accounts for 2-65% of the total mass of the pesticide formulation.

6. The pesticide formulation according to claim 4, characterized in that: The agriculturally acceptable auxiliary ingredients are selected from one or more of wetting agents, dispersants, disintegrators, emulsifiers, solvents, carriers, thickeners, defoamers, synergists and preservatives.

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