Plant growth regulator containing 14-hydroxyl brassinosterol and indolebutyric acid

Abstract

The invention belongs to the technical field of agriculture, and particularly relates to a plant growth regulator containing 14-hydroxyl brassinosterol and indolebutyric acid, the plant growth regulator comprises an active component A 14-hydroxyl brassinosterol, an active component B indolebutyric acid, and a biological enzyme preparation. The plant growth regulator provided by the invention can play an important role in the aspects of flowering, fruit setting and yield of plants, fruit quality, disease resistance and the like, improves the effects of 14-hydroxybrassinosteroid and indolebutyric acid, reduces the risk of adverse reaction or phytotoxicity, and has the advantages of wide application prospect, high market demand and huge commercial value.

Description

Technical Field

[0001] The invention belongs to the field of plant growth regulators, and specifically relates to a plant growth regulator containing 14-hydroxybrassinosteroid and indolebutyric acid and a preparation method thereof. Background Art

[0002] Plant growth regulators are chemical substances that can affect plant growth and development. They regulate the growth and development of plants by simulating or inhibiting the functions of natural hormones in plants. These regulators have a wide range of applications in agricultural production, including promoting seed germination, improving plant disease resistance and stress resistance, regulating plant flowering and fruiting, and improving fruit quality.

[0003] 14-hydroxylatedbrassinosteroid and 4-indol-3-ylbutyric acid are two commonly used plant growth regulators. 14-hydroxybrassinosteroid is a plant hormone that promotes cell elongation, thereby promoting plant growth. Indolebutyric acid is a growth inhibitor that inhibits plant growth and thus controls plant height. These two regulators are often used in the form of a mixture to improve the controllability and efficiency of plant growth. In addition, bioenzyme preparations are also an important agricultural input. They can decompose organic matter, release nutrients, and promote plant growth.

[0004] In the existing technology, 14-hydroxybrassinosteroid and indolebutyric acid have been used in combination, but the combination often fails to achieve the expected effect and is easily affected by extreme weather, which is not conducive to plant absorption. In addition, the dosage of plant growth regulators is strictly limited. A small dosage will not achieve the effect, and a large dosage will inhibit the growth of the plant itself or cause poor fruiting, which is easy to cause phytotoxicity.

[0005] The present application uses 14-hydroxy brassinosteroids and indolebutyric acid in combination with a biological enzyme preparation, which can improve the resistance of plants to extreme weather, thereby promoting a more obvious effect of growth. In addition, it can reduce the risk of using plant growth regulators, thereby reducing the occurrence of adverse reactions or pesticide damage. Therefore, how to effectively and safely use 14-hydroxy brassinosteroids and indolebutyric acid in combination with a biological enzyme preparation is still a problem that needs to be solved in this field. Summary of the invention

[0006] The purpose of the present invention is to provide a plant growth regulator and a preparation method thereof, which can strengthen the growth regulation of crops by compounding sodium nitrophenolate, ammonium ethyl hexanoate, biological enzyme preparations and auxiliary agents, so as to facilitate the spread of the plant growth regulator on the contact surface with the crops and improve the utilization rate of the drug.

[0007] To achieve the above purpose, the technical solution adopted by the present invention is: a plant growth regulator containing 14-hydroxybrassinosteroid and indolebutyric acid, wherein the plant growth regulator comprises: active ingredient A 14-hydroxybrassinosteroid, active ingredient B indolebutyric acid, and biological enzyme preparation.

[0008] Furthermore, the mass ratio of 14-hydroxybrassinosteroid to indolebutyric acid is 1:200 to 1:2000;

[0009] Furthermore, the mass ratio of 14-hydroxybrassinosteroid to indolebutyric acid is 1:500 to 1:1500;

[0010] Further, the mass ratio of 14-hydroxybrassinosteroid to indolebutyric acid is 1:499, 1:599, 1:699, 1:799, 1:899, 1:999, 1:1099, 1:1199, 1:1299, 1:1399, 1:1499;

[0011] Furthermore, the biological enzyme preparation is one or more of superoxide dismutase, saccharifying enzyme, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, β-glucanase and β-mannanase.

[0012] Furthermore, the biological enzyme preparation is a mixture of saccharifying enzyme, protease, amylase, pectinase or a mixture of saccharifying enzyme, protease, amylase, xylanase.

[0013] Furthermore, the mass ratio of the saccharifying enzyme, the protease, the amylase and the pectinase is 2:1:2:1;

[0014] Furthermore, the mass ratio of the saccharifying enzyme, the protease, the amylase and the pectinase is 2:2:1:1;

[0015] Furthermore, the mass ratio of the saccharifying enzyme, the protease, the amylase and the xylanase is 2:1:2:1;

[0016] Furthermore, the mass ratio of the saccharifying enzyme, the protease, the amylase and the xylanase is 2:2:1:1;

[0017] Furthermore, the plant growth regulator is composed of the following components in weight percentage: active ingredient A 0.0001% to 5%, active ingredient B 0.01% to 10%, biological enzyme preparation 0.5% to 10%, other pesticide adjuvants 1% to 20%, and deionized water to 100%;

[0018] Furthermore, the plant growth regulator is composed of the following components in weight percentage: active ingredient A 0.0001% to 2%, active ingredient B 0.1% to 10%, biological enzyme preparation 0.5% to 5%, other pesticide adjuvants 1% to 20%, and deionized water to 100%;

[0019] The other pesticide adjuvants may be selected from one or more of methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, propylene glycol methyl ether, dipropylene glycol methyl ether, ethylene glycol, propylene glycol, glycerol, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, and vegetable oil derivatives;

[0020] Furthermore, the other pesticide adjuvants may be selected from one or more of ethanol, isopropanol, dipropylene glycol methyl ether, ethylene glycol, propylene glycol, glycerol, vegetable oil, and vegetable oil derivatives;

[0021] Furthermore, the plant growth regulator can be used on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops;

[0022] Furthermore, the fruit trees include deciduous fruit trees, green fruit trees, and herbaceous fruit trees;

[0023] Further, deciduous fruit trees: apple, pear, peach, grape, jujube, persimmon, apricot, plum, plum, cherry, chestnut, walnut, hawthorn, pomegranate, hazelnut, sand apple, ginkgo, kiwi, blueberry, raspberry, fig, mulberry, papaya;

[0024] Further, green fruit trees: citrus, banana, lychee, longan, loquat, papaya, bayberry, olive, mango, carambola, coconut, guava, torreya, pistachio, sapodilla;

[0025] Further, herbaceous fruit trees: strawberry, pineapple, dragon fruit.

[0026] The present invention also provides a method for using the plant growth regulator as described above, specifically applying an effective dose to crops to be controlled or crop growth areas.

[0027] The plant growth regulator aqueous solution of the present invention has the following advantages:

[0028] 1) The plant growth regulator aqueous solution of the present invention is added with a biological enzyme preparation, which can decompose organic matter, release nutrients, promote plant growth, and thus improve the growth regulation ability of plants;

[0029] 2) The plant growth regulator of the present invention can improve the effects of 14-hydroxybrassinosteroid and indolebutyric acid in the application of crops, and reduce the risk of adverse reactions or phytotoxicity;

[0030] 3) The plant growth regulator of the present invention can play an important role in plant flowering, fruit setting, yield, fruit quality, disease resistance, etc. DETAILED DESCRIPTION

[0031] In order to make the present application easier to understand, the present application will be further described in detail below in conjunction with the examples, which are merely illustrative and are not intended to limit the scope of application of the present application. The raw materials or components used in the present application can be obtained through commercial routes or conventional methods unless otherwise specified.

[0032] Preparation Example

[0033] In order to better illustrate the present invention, the content of the present invention is further described below in conjunction with the embodiments. The parts in all the embodiments are parts by weight.

[0034] A plant growth regulator, comprising the following components in percentage by weight:

[0035] Active ingredient A 0.0001% ~ 5%;

[0036] Active ingredient B 0.01%~10%;

[0037] Biological enzyme preparation 0.5-10%;

[0038] Other pesticide adjuvants 1-20%;

[0039] Deionized water is added to 100%;

[0040] Preparation method: Active ingredient A, active ingredient B, bio-enzyme preparation, surfactant, solubilizer and deionized water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0041] A plant growth regulator, comprising the following components in percentage by weight:

[0042] 14-Hydroxybrassinosteroid 0.0001%~5%;

[0043] Indolebutyric acid 0.01%~10%;

[0044] Biological enzyme preparation 0.5-10%;

[0045] Other pesticide adjuvants 1-20%;

[0046] Deionized water is added to 100%;

[0047] Preparation method: 14-hydroxybrassinosteroid, indolebutyric acid, bioenzyme preparation, surfactant, solubilizer and deionized water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0048] A plant growth regulator, comprising the following components in percentage by weight:

[0049] 14-Hydroxybrassinosteroid 0.0001%~2%;

[0050] Indolebutyric acid 0.01%-5%;

[0051] Bio-enzyme preparation 3.6%;

[0052] Other pesticide adjuvants 1-15%;

[0053] Deionized water is added to 100%;

[0054] Preparation method: 14-hydroxybrassinosteroid, indolebutyric acid, bioenzyme preparation, surfactant, solubilizer and deionized water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0055] Preparation Example 1:

[0056] 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+2.495)

[0057] Recipe composition:

[0058] 0.005% 14-Hydroxybrassinosteroid

[0059] 2.495% Indolebutyric acid

[0060] 3.6% biological enzyme preparation (1.2% saccharifying enzyme, 1.2% protease, 0.6% amylase, 0.6% xylanase)

[0061] 6.5% fatty alcohol polyoxyethylene ether

[0062] 2.5% secondary alcohol polyoxyethylene ether

[0063] 6% benzenesulfonate anion (TXC)

[0064] 4.5% ethylene glycol,

[0065] Deionized water makes up the balance;

[0066] Preparation method: 14-hydroxybrassinosteroid, indolebutyric acid, bio-enzyme preparation, other pesticide adjuvants and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0067] Preparation Example 2:

[0068] 3.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+3.495)

[0069] Recipe composition:

[0070] 0.005% 14-Hydroxybrassinosteroid

[0071] 3.495% Indolebutyric acid

[0072] 3.6% biological enzyme preparation (1.2% saccharifying enzyme, 0.6% protease, 1.2% amylase, 0.6% xylanase)

[0073] 6.5% fatty alcohol polyoxyethylene ether

[0074] 2.5% secondary alcohol polyoxyethylene ether

[0075] 6% benzenesulfonate anion (TXC)

[0076] 4.5% Ethylene glycol

[0077] Deionized water makes up the balance;

[0078] Preparation method: Same as Preparation Example 1.

[0079] Preparation Example 3:

[0080] 3% 14-Hydroxybrassinosteroid·Indolebutyric Acid Aqueous Solution (0.003+2.997)

[0081] Recipe composition:

[0082] 0.003% 14-Hydroxybrassinosteroid

[0083] 2.997% Indolebutyric Acid

[0084] 3.6% biological enzyme preparation (1.2% saccharifying enzyme, 1.2% protease, 0.6% amylase, 0.6% pectinase)

[0085] 6.5% fatty alcohol polyoxyethylene ether

[0086] 2.5% secondary alcohol polyoxyethylene ether

[0087] 6% benzenesulfonate anion (TXC)

[0088] 4.5% Ethylene glycol

[0089] Deionized water makes up the balance;

[0090] Preparation method: Same as Preparation Example 1.

[0091] Preparation Example 4:

[0092] 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498)

[0093] Recipe composition:

[0094] 0.002% 14-Hydroxybrassinosteroid

[0095] 2.498% Indolebutyric acid

[0096] 3.6% biological enzyme preparation (1.2% saccharifying enzyme, 0.6% protease, 1.2% amylase, 0.6% pectinase)

[0097] 6.5% fatty alcohol polyoxyethylene ether

[0098] 2.5% secondary alcohol polyoxyethylene ether

[0099] 6% benzenesulfonate anion (TXC)

[0100] 4.5% Ethylene glycol

[0101] Deionized water makes up the balance;

[0102] Preparation method: Same as Preparation Example 1.

[0103] Comparative Example 1:

[0104] 3% 14-Hydroxybrassinosteroid·Indolebutyric Acid Aqueous Solution (0.003+2.997)

[0105] Recipe composition:

[0106] 0.003% 14-Hydroxybrassinosteroid

[0107] 2.997% Indolebutyric Acid

[0108] 6.5% fatty alcohol polyoxyethylene ether

[0109] 2.5% secondary alcohol polyoxyethylene ether

[0110] 6% benzenesulfonate anion (TXC)

[0111] 4.5% Ethylene glycol

[0112] Deionized water makes up the balance;

[0113] Preparation method: 14-hydroxybrassinosteroid, indolebutyric acid, other pesticide adjuvants and water are stirred and mixed evenly to prepare an aqueous product with a homogeneous and stable appearance.

[0114] Comparative Example 2:

[0115] 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498)

[0116] Recipe composition:

[0117] 0.002% 14-Hydroxybrassinosteroid

[0118] 2.498% Indolebutyric acid

[0119] 6.5% fatty alcohol polyoxyethylene ether

[0120] 2.5% secondary alcohol polyoxyethylene ether

[0121] 6% benzenesulfonate anion (TXC)

[0122] 4.5% Ethylene glycol

[0123] Deionized water makes up the balance;

[0124] Preparation method: Same as control example 1.

[0125] Example 1

[0126] Test time and location: Meishan City, 2023

[0127] Test target: citrus (navel orange), 10-year-old tree, plant spacing 2m×3.5m.

[0128] Experimental treatment: There were 9 experimental treatments in total, with 3 repetitions, 2 citrus trees in each plot, a total of 18 trees, and all fruit trees with basically the same growth and fruit bearing were selected for the experiment. An isolation row was set between each treatment in the experiment.

[0129] Experimental treatments and agents:

[0130] Table 1 Experimental treatment

[0131] serial number Treatment Agents Dilution multiple (times) Treatment 1 - Preparation Example 1 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+2.495) 3000 Treatment 2 - Preparation Example 2 3.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+3.495) 3000 Treatment 3 - Preparation Example 3 3% 14-Hydroxybrassinosteroid·Indolebutyric Acid Aqueous Solution (0.003+2.997) 3000 Treatment 4 - Preparation Example 4 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498) 3000 Treatment 5 - Control 1 3% 14-Hydroxybrassinosteroid·Indolebutyric Acid Aqueous Solution (0.003+2.997) 1500 Treatment 6 - Control 1 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498) 1500 Process 7 1.2% indolebutyric acid aqueous solution 1500 Process 8 0.0075% 14-Hydroxybrassinosteroid soluble solution 1500 Process 9 CK (clear water control) /

[0132] Application method: Spray the entire plant, with a focus on flowers and young fruits. Based on actual field conditions and farmers' water consumption habits, determine that 1.8L of water is used for each citrus plant. The amount of pesticide applied should be such that the leaves are evenly coated with the pesticide and there is a little dripping.

[0133] Application times: The experiment was conducted 3 times, once at the citrus flowering stage, young fruit stage and fruit expansion stage;

[0134] Survey indicators and methods: Before applying the pesticide, investigate the number of flower buds and blossoms. For each citrus tree, plant one main branch of a fixed size and with basically the same total number of flowers in the five directions of east, south, west, north and center to investigate the total number of flower buds and blossoms.

[0135] After the citrus fruits matured, the number of fruits on the fixed trunk branches was investigated tree by tree before harvest. At the same time, three citrus fruits (15 per tree) were randomly picked from the east, south, west, north and center of each tree, with a total of 30 fruits in each area, and the fruit diameter and single fruit weight were measured.

[0136] The experimental data were analyzed by multiple comparison using Duncan's new multiple range method. Different letters after the data in the same column indicate significant differences. Lowercase letters indicate (P=0.05). Data statistical analysis was completed with the help of SPSS20.0 software.

[0137] Results and Analysis:

[0138] The test results show that

[0139] The test results of the effects of different regulators on the fruit setting rate of citrus are shown in Table 2. The fruit setting number and fruit setting rate of treatment 3 with 3% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.003+2.997)-treatment 3 were the highest, with a fruit setting rate of 15.01%; the fruit setting number and fruit setting rate of treatment 1 with 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+2.495)-treatment 1, 3.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+3.495)-treatment 2, and 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498)-treatment 4 were second, with fruit setting rates of 13.46, 13.29, and 13.22, respectively.

[0140] Table 2 Experimental results of the effects of different regulators on citrus fruit setting rate

[0141]

[0142] Note: The data in the table are the average values ​​of 3 replicates (the same below). Different letters after the data in the same column indicate significant differences. Lowercase letters indicate (P=0.05).

[0143] The experimental results show that the fresh fruit diameter and single fruit weight of treatments with 3% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.003+2.997)-treatment 3 and 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498)-treatment 4 are the highest, respectively, followed by the fresh fruit diameter and single fruit weight of treatments with 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+2.495)-treatment 1 and 3.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+3.495)-treatment 2; for the other agents and dosage treatments, the fresh fruit diameter and fresh fruit weight are better than those of the clear water control.

[0144] Table 3 Experimental results of different regulators promoting citrus fruit growth

[0145]

[0146]

[0147] The actual yield of citrus showed that the average yields of the plots of 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+2.495)-treatment 1, 3.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.005+3.495)-treatment 2, 3% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.003+2.997)-treatment 3, and 2.5% 14-hydroxybrassinosteroid·indolebutyric acid aqueous solution (0.002+2.498)-treatment 4 were 48.29kg, 47.85kg, 48.62kg, and 49.29kg, respectively. The yields of the plots of the other treatments were all better than those of the clear water control.

[0148] Table 4 Experimental results of the effects of different regulators on citrus yield

[0149]

[0150] The plant growth regulator of the present application participates in regulating the growth and development of plants at all stages, which can increase crop yields and improve quality. The plant growth regulator of the present application can also induce citrus trees to enhance their ability to resist pests and diseases, reduce the occurrence of pests and diseases, and enhance tree nutrient absorption. In addition, its safe application combined with the supporting integrated application of other green production technologies can promote the efficient production of citrus, effectively improve the quality and safety of citrus fruits, and play an important role in promoting the zero growth action on the use of fertilizers and pesticides.

[0151] It should be noted that the embodiments described above are only used to explain the present application and do not constitute any limitation to the present application. The present application is described with reference to typical embodiments, but it should be understood that the words used therein are descriptive and explanatory words, rather than restrictive words. The present application may be modified as specified within the scope of the claims of the present application, and the present invention may be revised without departing from the scope and spirit of the present application. Although the present application described therein relates to specific methods, materials and embodiments, it does not mean that the present application is limited to the specific examples disclosed therein. On the contrary, the present application can be extended to all other methods and applications with the same functions.

Claims

1. A plant growth regulator containing 14-hydroxybrassinosteroid and indolebutyric acid, characterized in that: The plant growth regulator comprises: active ingredient A14-hydroxybrassinosteroid, active ingredient B indolebutyric acid, and biological enzyme preparation.

2. The plant growth regulator according to claim 1, characterized in that: The mass ratio of 14-hydroxybrassinosteroid to indolebutyric acid is 1:200 to 1:2000; Preferably, the mass ratio of 14-hydroxybrassinosteroid to indolebutyric acid is 1:500 to 1:1500.

3. The plant growth regulator according to claim 1, characterized in that: The biological enzyme preparation is one or more of superoxide dismutase, saccharifying enzyme, protease, urease, cellulase, lipase, phytase, pectinase, xylanase, beta glucanase and beta mannanase.

4. The plant growth regulator according to claim 3, characterized in that: The biological enzyme preparation is a mixture of saccharifying enzyme, protease, amylase and pectinase or a mixture of saccharifying enzyme, protease, amylase and xylanase.

5. The plant growth regulator according to claim 4, characterized in that: The mass ratio of the saccharifying enzyme, the protease, the amylase and the pectinase is 2:1:2:1 or 2:2:1:1; The mass ratio of the saccharifying enzyme, the protease, the amylase and the xylanase is 2:1:2:1 or 2:2:1:

1.

6. The plant growth regulator according to claim 1, characterized in that: The plant growth regulator is composed of the following components in percentage by weight: 0.0001% to 5% of active component A, 0.01% to 10% of active component B, 0.5% to 10% of biological enzyme preparation, 1% to 20% of other pesticide adjuvants, and deionized water to 100%.

7. The plant growth regulator according to claim 1, characterized in that: The other pesticide adjuvants can be selected from one or more of ethanol, isopropanol, dipropylene glycol methyl ether, ethylene glycol, propylene glycol, glycerol, vegetable oil, and vegetable oil derivatives.

8. The plant growth regulator according to claim 1, characterized in that: The plant growth regulator can be used on fruit trees, vegetables, ornamental plants, tea, cotton and cereal crops.

9. The plant growth regulator according to claim 8, characterized in that: The fruit trees include deciduous fruit trees, green fruit trees and herbaceous fruit trees.

10. The plant growth regulator according to claim 1, characterized in that: The aqueous solution is applied to the crops to be controlled or the areas where the crops grow in an effective dosage.

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